MD10G1239000.v1.1

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000360
Physical Location & Seq
Reverse (-)
33523306 .. 33527254
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1239000.v1.1.491

Sequence Viewer

Length: 2055 bp
ATGGCCGCTGCTACGTGGCGGAAGCTGAAGAAGTCGCTGATGCACAAGCTCTCATTCCACTCCTCCGATTCGTCGTCCACCTCGTCGGCGCCGGCGTCTCGGCCGCCTCGTCCTCCTCCTCTCGCCTTAACCTCCTCCGCCACCGTCCGCCCCTCTCCGTCGTCTCGCACCTCCTCCTCCTCCTCCTCCATCTCCTCCAAGCTCTCCCGCAGCTTCAGCTCTCGCATCTCCAAGAAAAATTGCGCCATTTGCCTGAGTAACCTGAGAGCAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCCCACTCTTTCCACTTCCTTTGCATTGCCAACAATGTCAAACATGGAAATATTTGCTGCCCCATCTGCCGTTCCAAGTGGAATGATGTGCCTTTCCAAGCTCCCACCAACGTTGCAGATACTCAACAAAACCACTTGGGTCACATCCATAACGGTTTTAACCATCGTTACCAGTCTCACCTCCCATTGCTGCCCCAGGTCCATCCCGAGCCACTTAGCTTCTCAGATGATGAGCCTCTCCCGTCCACCTCACCTAGTCGATTCTCTGGCCCCCAAAATGTTACAATCAGGACTTATACAGAGTACTCTGCTATCCCTGTTGCAGAATCTCGCCCAACATTTCCTGTTCTTGTCAGCGTTCGAGCACCACCCCTTCAAGGAGGTGCAAACAGCCATGGTCGTACCCCCATTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGTTTGGCACAAAAATTGCCCTTCTCAAGCGCGCTGTCAGATTTGTCATACAAAACTTGGGGCCTTATGACCGGCTTGCCATAGTTTCATTCTCAAATACTGCTAAAAGGGTCTTTCCTCTCCGAAGAATGACTGTTGATGGTCGTGAAAGCGCCTTTCTAGCAGTCGAGTCTCTTAGAGCACATGGTGGAACCGACATTGTTGGAGGACTCAAGAAAGGAGTTCAGGTCCTTGAAGAACGCCGGGCAAGGAATCCAGTTTCTAGCATCATCCTCTTATCTGATGGAAGAGACAATTGCATCTATGACTCACAGAATACTTCAAGTCCAAACCGGGTCTCTGAGTTCTTGAACCGATTGCCAGCTTCGGTACATTGTAGCAATGTTCCCATCCACACATTTGGGTTTGGCACTGACCATGATGCCACTACTATGCATGCTATATCTGATTCATCCAGTGGCACCTTTTCCTTCATTGAGTCAGTTGCTATGATACAAGATGCCTTTGCTATGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAACTTCGACTCACAGTTAGGTCAGCATCGCACGGGGTGCAGATTGTATCAATACCATCAGGGAGACATGTGAGTGAGATTTCTGACGAGTGTCAACAAGGTGCTGTTGATGTTGGAAATATGTATGCAGAAGAGGAGAAACAGTTTCTGGTGTACCTTTTAGTTCCAACATCATCAGCAACTGGAAACAGGGAAGGCATAAGGAAGACATCACTGTTGGATGTGTCATGCGTGTACAAGGATCTAGGCTCAAATGAGTCAACCCATGTGCAAGGTGAGAGAGTAGAGATATTAAGACCCAAGGTTTGGTCCCCTGCAGATGAGGCAGTGTGTTTGGAGGTCGACCGTCAGAGGAATAGAATTCTGGTGGCTGAAGGTATTGCAGAGGCACAAGCAATGGCTGAAATGGGAAATCTAGAAGGTGCACAGGCTATTTTGGCACAACGAATAGCAACCATCTTAACATCACCGGCAGCACAAGCTGGGGATGGTCTCTGTAATTGGCTTGAAGCTGAGCTTCGAGAAATCAGGGATAGAATGGCTAGTATGGAATCATACACACAGACCGGGCGTGCTTATGTTCTTTCAGGGTTGAGCTCACATTCATGGCAGAGAGCCACAACTAGGGGTGACATGACAACCCAGATTATGACCATGAATGAAGGTGGAAACTCACGCAGCAGCGCCGTTGGCTATGAAACACCTACTATGGTTAACATGGTAAGGATGTCGCAGAATCTTGGGGTTAATCACACGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA VWA_3 Vwaint zf-RING_11 zf-RING_2

Protein Analysis

685

Amino Acids

74.19

Weight (kDa)

8.7

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_11 PF17123 80 - 110 2.9e-09 RING-like zinc finger
zf-RING_2 PF13639 80 - 125 1.6e-06 Ring finger domain
VWA PF00092 239 - 412 6.4e-20 von Willebrand factor type A domain
VWA_3 PF13768 239 - 407 2.6e-16 von Willebrand factor type A domain
VWA_2 PF13519 240 - 345 1.9e-15 von Willebrand factor type A domain
Vwaint PF14624 594 - 676 2.5e-17 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 88, 1208
AccB7I CCANNNNNTGG 1 cut(s) 1599
AccI GTMKAC 1 cut(s) 1635
AccII CGCG 1 cut(s) 780
AciI CCGC 6 cut(s) 6, 19, 104, 138, 148, 208
AclI AACGTT 1 cut(s) 414
AclWI GGATC 1 cut(s) 1542
AcoI YGGCCR 2 cut(s) 3, 101
AcsI RAATTY 1 cut(s) 1653
AcuI CTGAAG 3 cut(s) 47, 199, 1686
AcyI GRCGYC 2 cut(s) 89, 95
AdeI CACNNNGTG 2 cut(s) 935, 1330
AfaI GTAC 5 cut(s) 608, 706, 1119, 1448, 1529
AfiI CCNNNNNNNGG 3 cut(s) 680, 1599, 1917
AflIII ACRYGT 1 cut(s) 1360
AgsI TTSAA 5 cut(s) 680, 983, 1071, 1099, 1802
AjnI CCWGG 1 cut(s) 498
Alw21I GWGCWC 5 cut(s) 305, 670, 931, 1720, 1892
Alw26I GTCTC 8 cut(s) 102, 168, 483, 924, 1032, 1090, 1351, 1790
Alw44I GTGCAC 1 cut(s) 1716
AlwI GGATC 1 cut(s) 1542
AlwNI CAGNNNCTG 2 cut(s) 1441, 1475
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 5 cut(s) 3, 101, 277, 571, 809
ApaLI GTGCAC 1 cut(s) 1716
ApeKI GCWGC 8 cut(s) 8, 210, 360, 493, 744, 1766, 1971, 1974
ApoI RAATTY 1 cut(s) 1653
ArsI GACNNNNNNTTYG 2 cut(s) 1581, 1613
AspLEI GCGC 6 cut(s) 91, 245, 780, 782, 902, 1979
AspS9I GGNCC 5 cut(s) 502, 572, 809, 976, 1602
AsuC2I CCSGG 3 cut(s) 992, 1082, 1861
AsuHPI GGTGA 5 cut(s) 473, 546, 1580, 1752, 1934
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 3 cut(s) 502, 976, 1602
BaeGI GKGCMC 1 cut(s) 1720
BanI GGYRCC 2 cut(s) 88, 1208
BanII GRGCYC 1 cut(s) 1892
BbsI GAAGAC 2 cut(s) 1265, 1505
Bbv12I GWGCWC 5 cut(s) 305, 670, 931, 1720, 1892
BbvI GCAGC 7 cut(s) 222, 347, 480, 756, 1778, 1983, 1986
BceAI ACGGC 2 cut(s) 357, 1964
BciT130I CCWGG 1 cut(s) 500
BcnI CCSGG 3 cut(s) 992, 1082, 1861
BcoDI GTCTC 8 cut(s) 102, 168, 483, 924, 1032, 1090, 1351, 1790
BfaI CTAG 7 cut(s) 558, 908, 1011, 1538, 1709, 1836, 1917
BfmI CTRYAG 1 cut(s) 1608
BfoI RGCGCY 3 cut(s) 92, 903, 1980
BlpI GCTNAGC 1 cut(s) 1806
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 4 cut(s) 500, 992, 1082, 1861
Bme18I GGWCC 3 cut(s) 502, 976, 1602
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 5 cut(s) 502, 572, 809, 976, 1602
BmiI GGNNCC 6 cut(s) 90, 574, 810, 940, 1210, 1604
BmrFI CCNGG 4 cut(s) 500, 992, 1082, 1861
BmsI GCATC 7 cut(s) 30, 234, 1023, 1056, 1159, 1237, 1328
BoxI GACNNNNGTC 1 cut(s) 1383
BpiI GAAGAC 2 cut(s) 1265, 1505
Bpu10I CCTNAGC 1 cut(s) 1290
Bpu1102I GCTNAGC 1 cut(s) 1806
BpuEI CTTGAG 2 cut(s) 758, 944
BpuMI CCSGG 3 cut(s) 992, 1082, 1861
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 2 cut(s) 89, 95
BsaI GGTCTC 2 cut(s) 1090, 1790
BsaJI CCNNGG 3 cut(s) 498, 697, 1593
BsaXI ACNNNNNCTCC 4 cut(s) 960, 990, 1421, 1451
Bsc4I CCNNNNNNNGG 3 cut(s) 680, 1599, 1917
Bse118I RCCGGY 3 cut(s) 91, 819, 1762
Bse1I ACTGG 4 cut(s) 475, 1004, 1203, 1480
Bse3DI GCAATG 4 cut(s) 327, 488, 1135, 1695
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 3 cut(s) 498, 697, 1593
BseGI GGATG 9 cut(s) 447, 505, 739, 1017, 1137, 1199, 1519, 1786, 2025
BseLI CCNNNNNNNGG 3 cut(s) 680, 1599, 1917
BseMI GCAATG 4 cut(s) 327, 488, 1135, 1695
BseMII CTCAG 8 cut(s) 245, 254, 288, 540, 1080, 1291, 1304, 1797
BseNI ACTGG 4 cut(s) 475, 1004, 1203, 1480
BsePI GCGCGC 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 1720
BseX3I CGGCCG 1 cut(s) 101
BseXI GCAGC 7 cut(s) 222, 347, 480, 756, 1778, 1983, 1986
BseYI CCCAGC 1 cut(s) 1775
BsgI GTGCAG 1 cut(s) 1352
Bsh1236I CGCG 1 cut(s) 780
Bsh1285I CGRYCG 2 cut(s) 104, 1639
BshFI GGCC 5 cut(s) 5, 103, 279, 573, 811
BshNI GGYRCC 2 cut(s) 88, 1208
BsiEI CGRYCG 2 cut(s) 104, 1639
BsiHKAI GWGCWC 5 cut(s) 305, 670, 931, 1720, 1892
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 6 cut(s) 92, 820, 991, 1081, 1763, 1860
BslFI GGGAC 1 cut(s) 1588
BslI CCNNNNNNNGG 3 cut(s) 680, 1599, 1917
BsmAI GTCTC 8 cut(s) 102, 168, 483, 924, 1032, 1090, 1351, 1790
BsmBI CGTCTC 2 cut(s) 102, 168
BsmFI GGGAC 1 cut(s) 1588
BsnI GGCC 5 cut(s) 5, 103, 279, 573, 811
Bso31I GGTCTC 2 cut(s) 1090, 1790
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 5 cut(s) 305, 670, 931, 1720, 1892
Bsp1407I TGTACA 1 cut(s) 1527
Bsp143I GATC 1 cut(s) 1534
Bsp1720I GCTNAGC 1 cut(s) 1806
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 6 cut(s) 6, 19, 104, 138, 148, 208
BspANI GGCC 5 cut(s) 5, 103, 279, 573, 811
BspCNI CTCAG 8 cut(s) 246, 255, 289, 539, 1081, 1290, 1303, 1798
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 6 cut(s) 90, 574, 810, 940, 1210, 1604
BspMAI CTGCAG 1 cut(s) 1612
BspPI GGATC 1 cut(s) 1542
BspT107I GGYRCC 2 cut(s) 88, 1208
BspTNI GGTCTC 2 cut(s) 1090, 1790
BsrDI GCAATG 4 cut(s) 327, 488, 1135, 1695
BsrFI RCCGGY 3 cut(s) 91, 819, 1762
BsrGI TGTACA 1 cut(s) 1527
BsrI ACTGG 4 cut(s) 475, 1004, 1203, 1480
BssAI RCCGGY 3 cut(s) 91, 819, 1762
BssECI CCNNGG 3 cut(s) 498, 697, 1593
BssHII GCGCGC 1 cut(s) 778
BssMI GATC 1 cut(s) 1534
BssNI GRCGYC 2 cut(s) 89, 95
BssT1I CCWWGG 2 cut(s) 697, 1593
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 9 cut(s) 145, 458, 727, 883, 1309, 1437, 1509, 1640, 2050
Bst6I CTCTTC 2 cut(s) 1030, 1419
BstACI GRCGYC 2 cut(s) 89, 95
BstAPI GCANNNNNTGC 1 cut(s) 1330
BstAUI TGTACA 1 cut(s) 1527
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 6 cut(s) 93, 780, 825, 1110, 1185, 1866
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 9 cut(s) 447, 505, 739, 1017, 1137, 1199, 1519, 1786, 2025
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 3 cut(s) 92, 903, 1980
BstHHI GCGC 6 cut(s) 91, 245, 780, 782, 902, 1979
BstKTI GATC 1 cut(s) 1537
BstMAI GTCTC 8 cut(s) 102, 168, 483, 924, 1032, 1090, 1351, 1790
BstMBI GATC 1 cut(s) 1534
BstMCI CGRYCG 2 cut(s) 104, 1639
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 3 cut(s) 751, 1187, 1364
BstPAI GACNNNNGTC 1 cut(s) 1383
BstSCI CCNGG 4 cut(s) 498, 990, 1080, 1859
BstSFI CTRYAG 1 cut(s) 1608
BstSLI GKGCMC 1 cut(s) 1720
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 7 cut(s) 222, 347, 480, 756, 1778, 1983, 1986
BstV2I GAAGAC 2 cut(s) 1265, 1505
BstX2I RGATCY 1 cut(s) 1534
BstXI CCANNNNNNTGG 1 cut(s) 1148
BstYI RGATCY 1 cut(s) 1534
BstZI CGGCCG 1 cut(s) 101
BsuRI GGCC 5 cut(s) 5, 103, 279, 573, 811
BtgI CCRYGG 1 cut(s) 697
BtgZI GCGATG 1 cut(s) 1305
BtsCI GGATG 9 cut(s) 447, 505, 739, 1017, 1137, 1199, 1519, 1786, 2025
BtsI GCAGTG 2 cut(s) 291, 1626
BtsIMutI CAGTG 6 cut(s) 291, 1158, 1210, 1285, 1505, 1626
Cac8I GCNNGC 6 cut(s) 93, 780, 825, 1110, 1185, 1866
CaiI CAGNNNCTG 2 cut(s) 1441, 1475
CfoI GCGC 6 cut(s) 91, 245, 780, 782, 902, 1979
Cfr10I RCCGGY 3 cut(s) 91, 819, 1762
Cfr13I GGNCC 5 cut(s) 502, 572, 809, 976, 1602
CseI GACGC 1 cut(s) 84
Csp6I GTAC 5 cut(s) 607, 705, 1118, 1447, 1528
CspCI CAANNNNNGTGG 4 cut(s) 305, 340, 397, 432
CviQI GTAC 5 cut(s) 607, 705, 1118, 1447, 1528
DinI GGCGCC 1 cut(s) 90
DpnI GATC 1 cut(s) 1536
DpnII GATC 1 cut(s) 1534
DraIII CACNNNGTG 2 cut(s) 935, 1330
EaeI YGGCCR 2 cut(s) 3, 101
EagI CGGCCG 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 1030, 1419
EarI CTCTTC 2 cut(s) 1030, 1419
EciI GGCGGA 3 cut(s) 34, 127, 137
Ecl136II GAGCTC 1 cut(s) 1890
EclXI CGGCCG 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 697, 1593
Eco24I GRGCYC 1 cut(s) 1892
Eco31I GGTCTC 2 cut(s) 1090, 1790
Eco47I GGWCC 3 cut(s) 502, 976, 1602
Eco52I CGGCCG 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 1890
Eco57I CTGAAG 3 cut(s) 47, 199, 1686
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 1890
EcoO109I RGGNCCY 2 cut(s) 809, 976
EcoRI GAATTC 1 cut(s) 1653
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 2 cut(s) 697, 1593
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 1892
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 2 cut(s) 697, 1593
Esp3I CGTCTC 2 cut(s) 102, 168
FalI AAGNNNNNCTT 2 cut(s) 1794, 1826
FaqI GGGAC 1 cut(s) 1588
FauI CCCGC 1 cut(s) 215
FblI GTMKAC 1 cut(s) 1635
FokI GGATG 9 cut(s) 434, 492, 746, 1004, 1124, 1186, 1526, 1793, 2032
FriOI GRGCYC 1 cut(s) 1892
FspBI CTAG 7 cut(s) 558, 908, 1011, 1538, 1709, 1836, 1917
GlaI GCGC 6 cut(s) 90, 244, 779, 781, 901, 1978
GsaI CCCAGC 1 cut(s) 1779
HaeII RGCGCY 3 cut(s) 92, 903, 1980
HaeIII GGCC 5 cut(s) 5, 103, 279, 573, 811
HapII CCGG 6 cut(s) 92, 820, 991, 1081, 1763, 1860
HgaI GACGC 1 cut(s) 84
HhaI GCGC 6 cut(s) 91, 245, 780, 782, 902, 1979
Hin1I GRCGYC 2 cut(s) 89, 95
Hin6I GCGC 6 cut(s) 89, 243, 778, 780, 900, 1977
HinP1I GCGC 6 cut(s) 89, 243, 778, 780, 900, 1977
HincII GTYRAC 4 cut(s) 1388, 1554, 1636, 2008
HindII GTYRAC 4 cut(s) 1388, 1554, 1636, 2008
HpaI GTTAAC 1 cut(s) 2008
HpaII CCGG 6 cut(s) 92, 820, 991, 1081, 1763, 1860
HphI GGTGA 5 cut(s) 473, 546, 1580, 1752, 1934
Hpy166II GTNNAC 9 cut(s) 78, 549, 1388, 1447, 1528, 1554, 1636, 1718, 2008
Hpy188I TCNGA 9 cut(s) 67, 529, 788, 872, 1030, 1090, 1195, 1378, 1644
Hpy188III TCNNGA 9 cut(s) 509, 592, 731, 893, 961, 1096, 1292, 1709, 1814
Hpy8I GTNNAC 9 cut(s) 78, 549, 1388, 1447, 1528, 1554, 1636, 1718, 2008
Hpy99I CGWCG 3 cut(s) 76, 88, 163
HpyAV CCTTC 7 cut(s) 686, 779, 1228, 1481, 1661, 1706, 1949
HpyCH4III ACNGT 9 cut(s) 145, 458, 727, 883, 1309, 1437, 1509, 1640, 2050
HpyCH4IV ACGT 2 cut(s) 14, 414
HpySE526I ACGT 2 cut(s) 14, 414
Hsp92I GRCGYC 2 cut(s) 89, 95
HspAI GCGC 6 cut(s) 89, 243, 778, 780, 900, 1977
KasI GGCGCC 1 cut(s) 88
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
KspAI GTTAAC 1 cut(s) 2008
Kzo9I GATC 1 cut(s) 1534
LmnI GCTCC 2 cut(s) 308, 409
Lsp1109I GCAGC 7 cut(s) 222, 347, 480, 756, 1778, 1983, 1986
LweI GCATC 7 cut(s) 30, 234, 1023, 1056, 1159, 1237, 1328
MaeI CTAG 7 cut(s) 558, 908, 1011, 1538, 1709, 1836, 1917
MaeII ACGT 2 cut(s) 14, 414
MaeIII GTNAC 6 cut(s) 257, 443, 470, 583, 721, 1922
MalI GATC 1 cut(s) 1536
MboI GATC 1 cut(s) 1534
MboII GAAGA 8 cut(s) 40, 280, 885, 995, 1047, 1265, 1436, 1510
MfeI CAATTG 1 cut(s) 1042
MflI RGATCY 1 cut(s) 1534
MhlI GDGCHC 5 cut(s) 305, 670, 931, 1720, 1892
MluCI AATT 5 cut(s) 238, 762, 1042, 1653, 1792
Mly113I GGCGCC 1 cut(s) 89
MlyI GAGTC 6 cut(s) 926, 951, 1049, 1235, 1296, 1559
MmeI TCCRAC 4 cut(s) 931, 1387, 1484, 1491
Mph1103I ATGCAT 1 cut(s) 1185
MreI CGCCGGCG 1 cut(s) 91
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 6 cut(s) 128, 462, 1586, 1754, 2007, 2040
MslI CAYNNNNRTG 3 cut(s) 1178, 1365, 1897
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 6 cut(s) 92, 820, 991, 1081, 1763, 1860
MspR9I CCNGG 4 cut(s) 500, 992, 1082, 1861
MunI CAATTG 1 cut(s) 1042
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 780
NaeI GCCGGC 1 cut(s) 93
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 3 cut(s) 992, 1082, 1861
NcoI CCATGG 1 cut(s) 697
NdeII GATC 1 cut(s) 1534
NgoMIV GCCGGC 1 cut(s) 91
NlaIV GGNNCC 6 cut(s) 90, 574, 810, 940, 1210, 1604
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 443, 1922
NsiI ATGCAT 1 cut(s) 1185
NspI RCATGY 3 cut(s) 751, 1187, 1364
PaeI GCATGC 1 cut(s) 1187
PauI GCGCGC 1 cut(s) 778
PciI ACATGT 1 cut(s) 1360
PcsI WCGNNNNNNNCGW 3 cut(s) 80, 92, 106
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 7 cut(s) 68, 564, 629, 1000, 1196, 1844, 2029
PflMI CCANNNNNTGG 1 cut(s) 1599
PleI GAGTC 6 cut(s) 925, 951, 1049, 1234, 1296, 1558
PluTI GGCGCC 1 cut(s) 92
PpsI GAGTC 6 cut(s) 925, 951, 1049, 1234, 1296, 1558
Ppu21I YACGTR 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 976
PscI ACATGT 1 cut(s) 1360
PshAI GACNNNNGTC 1 cut(s) 1383
Psp124BI GAGCTC 1 cut(s) 1892
Psp1406I AACGTT 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 976
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 1775
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 6 cut(s) 90, 574, 810, 940, 1210, 1604
PspPI GGNCC 5 cut(s) 502, 572, 809, 976, 1602
PspPPI RGGWCCY 1 cut(s) 976
PstI CTGCAG 1 cut(s) 1612
PstNI CAGNNNCTG 2 cut(s) 1441, 1475
PsuI RGATCY 1 cut(s) 1534
PteI GCGCGC 1 cut(s) 778
RsaI GTAC 5 cut(s) 608, 706, 1119, 1448, 1529
RsaNI GTAC 5 cut(s) 607, 705, 1118, 1447, 1528
RseI CAYNNNNRTG 3 cut(s) 1178, 1365, 1897
SacI GAGCTC 1 cut(s) 1892
SalI GTCGAC 1 cut(s) 1634
SaqAI TTAA 6 cut(s) 128, 462, 1586, 1754, 2007, 2040
Sau3AI GATC 1 cut(s) 1534
Sau96I GGNCC 5 cut(s) 502, 572, 809, 976, 1602
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 6 cut(s) 926, 951, 1049, 1235, 1296, 1559
ScrFI CCNGG 4 cut(s) 500, 992, 1082, 1861
SduI GDGCHC 5 cut(s) 305, 670, 931, 1720, 1892
SfaNI GCATC 7 cut(s) 30, 234, 1023, 1056, 1159, 1237, 1328
SfcI CTRYAG 1 cut(s) 1608
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 91
SinI GGWCC 3 cut(s) 502, 976, 1602
SmiMI CAYNNNNRTG 3 cut(s) 1178, 1365, 1897
SmlI CTYRAG 2 cut(s) 773, 959
SmoI CTYRAG 2 cut(s) 773, 959
SphI GCATGC 1 cut(s) 1187
Sse9I AATT 5 cut(s) 238, 762, 1042, 1653, 1792
SsiI CCGC 6 cut(s) 6, 19, 104, 138, 148, 208
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 355
SspMI CTAG 7 cut(s) 558, 908, 1011, 1538, 1709, 1836, 1917
SstI GAGCTC 1 cut(s) 1892
StyD4I CCNGG 4 cut(s) 498, 990, 1080, 1859
StyI CCWWGG 2 cut(s) 697, 1593
TaaI ACNGT 9 cut(s) 145, 458, 727, 883, 1309, 1437, 1509, 1640, 2050
TaiI ACGT 2 cut(s) 17, 417
TaqI TCGA 6 cut(s) 562, 664, 915, 1300, 1635, 1813
TasI AATT 5 cut(s) 238, 762, 1042, 1653, 1792
TatI WGTACW 2 cut(s) 606, 1527
TauI GCSGC 2 cut(s) 8, 106
TfiI GAWTC 7 cut(s) 68, 564, 629, 1000, 1196, 1844, 2029
Tru1I TTAA 6 cut(s) 128, 462, 1586, 1754, 2007, 2040
Tru9I TTAA 6 cut(s) 128, 462, 1586, 1754, 2007, 2040
TscAI CASTG 6 cut(s) 298, 1165, 1210, 1285, 1512, 1626
TseFI GTSAC 2 cut(s) 443, 1922
TseI GCWGC 8 cut(s) 8, 210, 360, 493, 744, 1766, 1971, 1974
Tsp45I GTSAC 2 cut(s) 443, 1922
TspDTI ATGAA 7 cut(s) 825, 1188, 1210, 1887, 1964, 1968, 2004
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 6 cut(s) 298, 1165, 1210, 1285, 1512, 1626
Van91I CCANNNNNTGG 1 cut(s) 1599
VneI GTGCAC 1 cut(s) 1716
VpaK11BI GGWCC 3 cut(s) 502, 976, 1602
XapI RAATTY 1 cut(s) 1653
XbaI TCTAGA 1 cut(s) 1708
XceI RCATGY 3 cut(s) 751, 1187, 1364
XmiI GTMKAC 1 cut(s) 1635
XspI CTAG 7 cut(s) 558, 908, 1011, 1538, 1709, 1836, 1917
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.