Rh5DG138900

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000360
Physical Location & Seq
Forward (+)
14002438 .. 14005870
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG138900.1

Sequence Viewer

Length: 1878 bp
ATGAACAATTGCGGAATTTGCAGGAGTAGCCTGACAAGAGGGCTAAGCGAAGCCATCTTCACTGCTGAGTGCTCGCACGTTTTCCACTACCATTGCATTCTCAAAAATGTCCAGCAAGGAAATCTTTGCTGCCCTGTCTGCCAAGCAAAGTGGAATAAGGATAATGTACCTTTCCAGGTTCCCCCTCCTCAGAACAACAACTTGGGCACAATCCCTTGTCATTTCACATTCCCATCACAGCAGCCACCCCATTGGTTCTATCCTCAGTACTCATCCATGCCAGAACCCGAGTACCCTCCTGAGCCACCTACCTTCTCTGACGATGAACCTCTCCCGTCGTGCACCTCATCCATTCAATCCTCTGCCCCGCAGAATATAACAGTCAAGACTCATACGGAGTCATCTGCTATCTCTTCTGCAGAATCAATCCCAAATTTTCCTGTCCTTGTCAGCATTCTTGCACCACCCCTTCAGGATTCTGAGGGCCATGGTCGTACGCCCATTGACCTTGTGACAGTTCTGGATGTCAGTGGCAGCATGGAAGGCACAAAGCTTTCCCTACTCAAGCAAGCTGTCACATTTGTCATACAAAACTTAGGGCCTTCGGATAGACTTTCTGTAGTTTCGTTCTCATCAAGTTGTAGAAGAGTCTTTCCTCTCCAGAGAATGTCTGTTGATGGCCATGAAAGTGCCATCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCTGAAGGACTCAAGAAAGGAATTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGCATCATCCTCTTATCTGATGGCATAGATACATACAATCGAACTGCAAACCGACTGTTGAACCAGTTGCCTGATTCGATTCGTTCTAGCAACATGCAACATGAAATCCCAGTCCACACATTTGGATTTGGCTACGACCATGATCCCAACATTATGCATGCTATATCTGATCTGTCAGGTGGTACCTTTTCGTTCATTGAATCAGTTGGAATGATACAAGACTCCTTTGCTCTGTGTATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCGCCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACACGTGAATGAGATTTCTGATGAGGGTCAACAAGGTGTTGTTCACGTTGGAAATATGTACGCAGAAGAGGAGAAACAATTTCTGGTCTACCTCTTAGTTCCAGAATCCTCAGGTCCGTATACTAAGATGTCATTGTTAGATGTGTTATGCATGTACAAAGATCTAGCTTCCAATGAACTCATCCGGGTGCAAGGTGAGAAAATGGAGATACTGAGACCTGAGGTTTGTTCCCCTGCTGAAAAGGCAGTGTCTTTGGAGGTTGATCGGCAGCGGAACAGAATTTTGGTGGCTGAAGCTATTGCTGAGGCACAAAGGTTGGCTGAGATGGGAAATATTGAGGGTGCACAGGCTCTACTGGCTCAACGGAAAGAAATTCTCTTAACATCTCCAGCATCCCAAGCTGGGGATTATCACAGTAATTTGTTTGAAACTGAGCTTAGAGAAATCATGGATAGAATGGCATCTATGAAGTTGTATAAACAAACCGGGCGTGCTTACGCTCTTGCAGGAATCAGTTCCCATAAATCACAAAGGGCCAGTGCTAGGGGTGACACAAGAGCCTCCTGTCTCAATTCGGGTTCAGTTGTCTCCAGGAAGAGGGGTCTCCGTAGTCGCAACAGTACCTACAGTTTTGCTTCAGGTGAGTTGGGTCAACCACCTGCCGCAGTTGGTGCTTTCGAAACACCTGCTATGGTCAGAATGGTAAGAAAATCACAGAATAGAAATCAACCTGGGCCATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

625

Amino Acids

68.33

Weight (kDa)

6.37

Isoelectric Point (pI)

64.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 2 - 48 5.8e-06 Ring finger domain
zf-RING_11 PF17123 3 - 33 1.3e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 4 - 47 4.9e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 165 - 290 1.6e-08 VWA domain containing CoxE-like protein
VWA PF00092 169 - 334 1.2e-21 von Willebrand factor type A domain
VWA_3 PF13768 169 - 332 3.7e-18 von Willebrand factor type A domain
VWA_2 PF13519 170 - 275 1.2e-18 von Willebrand factor type A domain
WAV3_C PF25243 465 - 532 1.9e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 521 - 617 5.9e-10 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 710, 1801, 1828
Acc36I ACCTGC 3 cut(s) 710, 1801, 1828
Acc65I GGTACC 1 cut(s) 983
AccB1I GGYRCC 1 cut(s) 983
AccI GTMKAC 2 cut(s) 1221, 1253
AciI CCGC 4 cut(s) 12, 368, 1405, 1797
AclWI GGATC 1 cut(s) 938
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 4 cut(s) 15, 433, 1413, 1506
AcuI CTGAAG 4 cut(s) 455, 762, 1446, 1755
AcvI CACGTG 1 cut(s) 1138
AfaI GTAC 8 cut(s) 168, 269, 293, 496, 985, 1193, 1289, 1756
AfiI CCNNNNNNNGG 4 cut(s) 1536, 1537, 1677, 1731
AflIII ACRYGT 1 cut(s) 1135
AgsI TTSAA 5 cut(s) 356, 773, 862, 1001, 1562
AjnI CCWGG 4 cut(s) 174, 1099, 1724, 1864
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 1400, 1432
AluBI AGCT 6 cut(s) 553, 572, 1301, 1430, 1535, 1570
AluI AGCT 6 cut(s) 553, 572, 1301, 1430, 1535, 1570
Alw21I GWGCWC 3 cut(s) 74, 344, 1480
Alw26I GTCTC 6 cut(s) 714, 1126, 1342, 1706, 1726, 1742
Alw44I GTGCAC 2 cut(s) 340, 1476
AlwI GGATC 1 cut(s) 938
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 5 cut(s) 484, 599, 679, 1668, 1868
ApaLI GTGCAC 2 cut(s) 340, 1476
ApeKI GCWGC 4 cut(s) 129, 241, 534, 1402
ApoI RAATTY 4 cut(s) 15, 433, 1413, 1506
ArsI GACNNNNNNTTYG 2 cut(s) 1341, 1373
Asp700I GAANNNNTTC 1 cut(s) 1648
Asp718I GGTACC 1 cut(s) 983
AspS9I GGNCC 6 cut(s) 484, 599, 766, 1247, 1668, 1868
AsuC2I CCSGG 2 cut(s) 1319, 1621
AsuHPI GGTGA 4 cut(s) 1117, 1340, 1694, 1787
AsuII TTCGAA 1 cut(s) 1812
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 2 cut(s) 766, 1247
AxyI CCTNAGG 2 cut(s) 1243, 1353
BaeGI GKGCMC 3 cut(s) 209, 344, 1480
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BalI TGGCCA 1 cut(s) 681
BanI GGYRCC 1 cut(s) 983
BbrPI CACGTG 1 cut(s) 1138
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 3 cut(s) 74, 344, 1480
BbvCI CCTCAGC 1 cut(s) 1437
BbvI GCAGC 4 cut(s) 116, 253, 546, 1414
BccI CCATC 7 cut(s) 62, 241, 671, 701, 815, 1132, 1453
BcgI CGANNNNNNTGC 2 cut(s) 1802, 1836
BciT130I CCWGG 4 cut(s) 176, 1101, 1726, 1866
BcnI CCSGG 2 cut(s) 1319, 1621
BcoDI GTCTC 6 cut(s) 714, 1126, 1342, 1706, 1726, 1742
BfaI CTAG 4 cut(s) 698, 888, 1298, 1677
BfmI CTRYAG 3 cut(s) 417, 618, 1759
BfuAI ACCTGC 3 cut(s) 710, 1801, 1828
BglII AGATCT 1 cut(s) 1294
BisI GCNGC 5 cut(s) 130, 242, 535, 1403, 1797
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 5 cut(s) 131, 243, 536, 1404, 1798
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 6 cut(s) 176, 1101, 1319, 1621, 1726, 1866
Bme18I GGWCC 2 cut(s) 766, 1247
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 6 cut(s) 484, 599, 766, 1247, 1668, 1868
BmiI GGNNCC 2 cut(s) 180, 985
BmrFI CCNGG 6 cut(s) 176, 1101, 1319, 1621, 1726, 1866
BmrI ACTGGG 2 cut(s) 788, 905
BmsI GCATC 4 cut(s) 813, 1103, 1535, 1604
BmuI ACTGGG 2 cut(s) 788, 905
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 3 cut(s) 644, 1506, 1708
Bpu10I CCTNAGC 3 cut(s) 300, 1065, 1437
Bpu1102I GCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 1812
BpuEI CTTGAG 2 cut(s) 548, 734
BpuMI CCSGG 2 cut(s) 1319, 1621
BsaAI YACGTR 1 cut(s) 1138
BsaI GGTCTC 2 cut(s) 1342, 1742
BsaJI CCNNGG 3 cut(s) 487, 1100, 1865
Bsc4I CCNNNNNNNGG 4 cut(s) 1536, 1537, 1677, 1731
Bse1I ACTGG 5 cut(s) 794, 865, 911, 1494, 1671
Bse21I CCTNAGG 2 cut(s) 1243, 1353
Bse3DI GCAATG 2 cut(s) 91, 735
BseBI CCWGG 4 cut(s) 176, 1101, 1726, 1866
BseDI CCNNGG 3 cut(s) 487, 1100, 1865
BseGI GGATG 7 cut(s) 272, 347, 529, 693, 807, 1314, 1526
BseLI CCNNNNNNNGG 4 cut(s) 1536, 1537, 1677, 1731
BseMI GCAATG 2 cut(s) 91, 735
BseNI ACTGG 5 cut(s) 794, 865, 911, 1494, 1671
BseRI GAGGAG 2 cut(s) 177, 1217
BseSI GKGCMC 3 cut(s) 209, 344, 1480
BseXI GCAGC 4 cut(s) 116, 253, 546, 1414
BseYI CCCAGC 1 cut(s) 1535
BshFI GGCC 5 cut(s) 486, 601, 681, 1670, 1870
BshNI GGYRCC 1 cut(s) 983
BsiHKAI GWGCWC 3 cut(s) 74, 344, 1480
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 1318, 1620
BsiWI CGTACG 1 cut(s) 494
BslI CCNNNNNNNGG 4 cut(s) 1536, 1537, 1677, 1731
BsmAI GTCTC 6 cut(s) 714, 1126, 1342, 1706, 1726, 1742
BsmI GAATGC 2 cut(s) 96, 453
BsnI GGCC 5 cut(s) 486, 601, 681, 1670, 1870
Bso31I GGTCTC 2 cut(s) 1342, 1742
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 1812
Bsp1286I GDGCHC 4 cut(s) 74, 209, 344, 1480
Bsp1407I TGTACA 1 cut(s) 1287
Bsp143I GATC 5 cut(s) 775, 943, 970, 1294, 1396
Bsp1720I GCTNAGC 1 cut(s) 44
Bsp19I CCATGG 1 cut(s) 487
BspACI CCGC 4 cut(s) 12, 368, 1405, 1797
BspANI GGCC 5 cut(s) 486, 601, 681, 1670, 1870
BspLI GGNNCC 2 cut(s) 180, 985
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 3 cut(s) 710, 1801, 1828
BspPI GGATC 1 cut(s) 938
BspT104I TTCGAA 1 cut(s) 1812
BspT107I GGYRCC 1 cut(s) 983
BspTNI GGTCTC 2 cut(s) 1342, 1742
BsrDI GCAATG 2 cut(s) 91, 735
BsrGI TGTACA 1 cut(s) 1287
BsrI ACTGG 5 cut(s) 794, 865, 911, 1494, 1671
BssECI CCNNGG 3 cut(s) 487, 1100, 1865
BssMI GATC 5 cut(s) 775, 943, 970, 1294, 1396
BssNAI GTATAC 1 cut(s) 1254
BssT1I CCWWGG 1 cut(s) 487
Bst1107I GTATAC 1 cut(s) 1254
Bst2UI CCWGG 4 cut(s) 176, 1101, 1726, 1866
Bst4CI ACNGT 7 cut(s) 382, 517, 858, 1084, 1550, 1754, 1763
Bst6I CTCTTC 4 cut(s) 418, 640, 1194, 1724
BstAPI GCANNNNNTGC 2 cut(s) 725, 1805
BstAUI TGTACA 1 cut(s) 1287
BstBAI YACGTR 1 cut(s) 1138
BstBI TTCGAA 1 cut(s) 1812
BstC8I GCNNGC 4 cut(s) 74, 570, 960, 1626
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 7 cut(s) 272, 347, 529, 693, 807, 1314, 1526
BstKTI GATC 5 cut(s) 778, 946, 973, 1297, 1399
BstMAI GTCTC 6 cut(s) 714, 1126, 1342, 1706, 1726, 1742
BstMBI GATC 5 cut(s) 775, 943, 970, 1294, 1396
BstNI CCWGG 4 cut(s) 176, 1101, 1726, 1866
BstNSI RCATGY 3 cut(s) 898, 962, 1288
BstSCI CCNGG 6 cut(s) 174, 1099, 1317, 1619, 1724, 1864
BstSFI CTRYAG 3 cut(s) 417, 618, 1759
BstSLI GKGCMC 3 cut(s) 209, 344, 1480
BstV1I GCAGC 4 cut(s) 116, 253, 546, 1414
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 1 cut(s) 1294
BstXI CCANNNNNNTGG 2 cut(s) 252, 923
BstYI RGATCY 1 cut(s) 1294
BstZ17I GTATAC 1 cut(s) 1254
Bsu36I CCTNAGG 2 cut(s) 1243, 1353
BsuRI GGCC 5 cut(s) 486, 601, 681, 1670, 1870
BtgI CCRYGG 1 cut(s) 487
BtgZI GCGATG 1 cut(s) 1080
BtsCI GGATG 7 cut(s) 272, 347, 529, 693, 807, 1314, 1526
BtsI GCAGTG 2 cut(s) 60, 1386
BtsIMutI CAGTG 5 cut(s) 60, 535, 1060, 1386, 1678
BveI ACCTGC 3 cut(s) 710, 1801, 1828
Cac8I GCNNGC 4 cut(s) 74, 570, 960, 1626
Cfr13I GGNCC 6 cut(s) 484, 599, 766, 1247, 1668, 1868
Csp6I GTAC 8 cut(s) 167, 268, 292, 495, 984, 1192, 1288, 1755
CspCI CAANNNNNGTGG 4 cut(s) 74, 109, 131, 166
CviQI GTAC 8 cut(s) 167, 268, 292, 495, 984, 1192, 1288, 1755
DpnI GATC 5 cut(s) 777, 945, 972, 1296, 1398
DpnII GATC 5 cut(s) 775, 943, 970, 1294, 1396
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 4 cut(s) 418, 640, 1194, 1724
EarI CTCTTC 4 cut(s) 418, 640, 1194, 1724
Eco130I CCWWGG 1 cut(s) 487
Eco31I GGTCTC 2 cut(s) 1342, 1742
Eco47I GGWCC 2 cut(s) 766, 1247
Eco57I CTGAAG 4 cut(s) 455, 762, 1446, 1755
Eco72I CACGTG 1 cut(s) 1138
Eco81I CCTNAGG 2 cut(s) 1243, 1353
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 2 cut(s) 599, 766
EcoRII CCWGG 4 cut(s) 174, 1099, 1724, 1864
EcoT14I CCWWGG 1 cut(s) 487
EcoT22I ATGCAT 2 cut(s) 960, 1286
ErhI CCWWGG 1 cut(s) 487
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FauI CCCGC 1 cut(s) 375
FblI GTMKAC 2 cut(s) 1221, 1253
Fnu4HI GCNGC 5 cut(s) 130, 242, 535, 1403, 1797
FokI GGATG 7 cut(s) 259, 334, 536, 680, 794, 1301, 1513
Fsp4HI GCNGC 5 cut(s) 130, 242, 535, 1403, 1797
FspBI CTAG 4 cut(s) 698, 888, 1298, 1677
GluI GCNGC 5 cut(s) 130, 242, 535, 1403, 1797
GsaI CCCAGC 1 cut(s) 1539
GsuI CTGGAG 3 cut(s) 644, 1506, 1708
HaeIII GGCC 5 cut(s) 486, 601, 681, 1670, 1870
HapII CCGG 2 cut(s) 1318, 1620
HincII GTYRAC 2 cut(s) 1163, 1787
HindII GTYRAC 2 cut(s) 1163, 1787
HindIII AAGCTT 1 cut(s) 551
HpaII CCGG 2 cut(s) 1318, 1620
HphI GGTGA 4 cut(s) 1117, 1340, 1694, 1787
Hpy166II GTNNAC 8 cut(s) 342, 916, 1163, 1177, 1222, 1254, 1478, 1787
Hpy188I TCNGA 8 cut(s) 192, 319, 481, 607, 820, 970, 1153, 1832
Hpy188III TCNNGA 8 cut(s) 299, 385, 473, 521, 661, 751, 1067, 1235
Hpy8I GTNNAC 8 cut(s) 342, 916, 1163, 1177, 1222, 1254, 1478, 1787
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 6 cut(s) 322, 479, 536, 612, 737, 774
HpyCH4III ACNGT 7 cut(s) 382, 517, 858, 1084, 1550, 1754, 1763
HpyCH4IV ACGT 3 cut(s) 78, 1137, 1179
HpySE526I ACGT 3 cut(s) 78, 1137, 1179
KpnI GGTACC 1 cut(s) 987
Kzo9I GATC 5 cut(s) 775, 943, 970, 1294, 1396
Lsp1109I GCAGC 4 cut(s) 116, 253, 546, 1414
LweI GCATC 4 cut(s) 813, 1103, 1535, 1604
MaeI CTAG 4 cut(s) 698, 888, 1298, 1677
MaeII ACGT 3 cut(s) 78, 1137, 1179
MaeIII GTNAC 3 cut(s) 511, 574, 1682
MalI GATC 5 cut(s) 777, 945, 972, 1296, 1398
MboI GATC 5 cut(s) 775, 943, 970, 1294, 1396
MboII GAAGA 8 cut(s) 49, 405, 657, 785, 1040, 1120, 1211, 1741
MfeI CAATTG 1 cut(s) 7
MflI RGATCY 1 cut(s) 1294
MhlI GDGCHC 4 cut(s) 74, 209, 344, 1480
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 9 cut(s) 7, 15, 433, 759, 1211, 1413, 1506, 1552, 1705
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 6 cut(s) 382, 407, 657, 716, 741, 1016
MmeI TCCRAC 2 cut(s) 988, 1162
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 2 cut(s) 960, 1286
MroXI GAANNNNTTC 1 cut(s) 1648
MscI TGGCCA 1 cut(s) 681
MseI TTAA 2 cut(s) 762, 1514
MslI CAYNNNNRTG 3 cut(s) 687, 1140, 1319
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 1405
MspI CCGG 2 cut(s) 1318, 1620
MspR9I CCNGG 6 cut(s) 176, 1101, 1319, 1621, 1726, 1866
MunI CAATTG 1 cut(s) 7
Mva1269I GAATGC 2 cut(s) 96, 453
MvaI CCWGG 4 cut(s) 176, 1101, 1726, 1866
NciI CCSGG 2 cut(s) 1319, 1621
NcoI CCATGG 1 cut(s) 487
NdeII GATC 5 cut(s) 775, 943, 970, 1294, 1396
NlaIV GGNNCC 2 cut(s) 180, 985
NmuCI GTSAC 3 cut(s) 511, 574, 1682
NsiI ATGCAT 2 cut(s) 960, 1286
NspI RCATGY 3 cut(s) 898, 962, 1288
NspV TTCGAA 1 cut(s) 1812
PaeI GCATGC 1 cut(s) 962
PaqCI CACCTGC 3 cut(s) 710, 1801, 1828
PctI GAATGC 2 cut(s) 96, 453
PdmI GAANNNNTTC 1 cut(s) 1648
PfeI GAWTC 7 cut(s) 422, 476, 875, 880, 1001, 1238, 1644
Pfl23II CGTACG 1 cut(s) 494
PfoI TCCNGGA 1 cut(s) 1724
PkrI GCNGC 5 cut(s) 131, 243, 536, 1404, 1798
PleI GAGTC 6 cut(s) 382, 406, 656, 715, 741, 1016
PmaCI CACGTG 1 cut(s) 1138
PmlI CACGTG 1 cut(s) 1138
PpsI GAGTC 6 cut(s) 382, 406, 656, 715, 741, 1016
Ppu21I YACGTR 1 cut(s) 1138
PpuMI RGGWCCY 1 cut(s) 766
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 4 cut(s) 174, 1099, 1724, 1864
PspCI CACGTG 1 cut(s) 1138
PspFI CCCAGC 1 cut(s) 1535
PspGI CCWGG 4 cut(s) 174, 1099, 1724, 1864
PspLI CGTACG 1 cut(s) 494
PspN4I GGNNCC 2 cut(s) 180, 985
PspPI GGNCC 6 cut(s) 484, 599, 766, 1247, 1668, 1868
PspPPI RGGWCCY 1 cut(s) 766
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 1294
RsaI GTAC 8 cut(s) 168, 269, 293, 496, 985, 1193, 1289, 1756
RsaNI GTAC 8 cut(s) 167, 268, 292, 495, 984, 1192, 1288, 1755
RseI CAYNNNNRTG 3 cut(s) 687, 1140, 1319
SaqAI TTAA 2 cut(s) 762, 1514
SatI GCNGC 5 cut(s) 130, 242, 535, 1403, 1797
Sau3AI GATC 5 cut(s) 775, 943, 970, 1294, 1396
Sau96I GGNCC 6 cut(s) 484, 599, 766, 1247, 1668, 1868
ScaI AGTACT 1 cut(s) 269
SchI GAGTC 6 cut(s) 382, 407, 657, 716, 741, 1016
ScrFI CCNGG 6 cut(s) 176, 1101, 1319, 1621, 1726, 1866
SduI GDGCHC 4 cut(s) 74, 209, 344, 1480
SfaNI GCATC 4 cut(s) 813, 1103, 1535, 1604
SfcI CTRYAG 3 cut(s) 417, 618, 1759
SfuI TTCGAA 1 cut(s) 1812
SinI GGWCC 2 cut(s) 766, 1247
SmiMI CAYNNNNRTG 3 cut(s) 687, 1140, 1319
SmlI CTYRAG 2 cut(s) 563, 749
SmoI CTYRAG 2 cut(s) 563, 749
SphI GCATGC 1 cut(s) 962
Sse9I AATT 9 cut(s) 7, 15, 433, 759, 1211, 1413, 1506, 1552, 1705
SsiI CCGC 4 cut(s) 12, 368, 1405, 1797
SspI AATATT 1 cut(s) 1468
SspMI CTAG 4 cut(s) 698, 888, 1298, 1677
StyD4I CCNGG 6 cut(s) 174, 1099, 1317, 1619, 1724, 1864
StyI CCWWGG 1 cut(s) 487
TaaI ACNGT 7 cut(s) 382, 517, 858, 1084, 1550, 1754, 1763
TaiI ACGT 3 cut(s) 81, 1140, 1182
TaqI TCGA 4 cut(s) 778, 841, 878, 1812
TasI AATT 9 cut(s) 7, 15, 433, 759, 1211, 1413, 1506, 1552, 1705
TatI WGTACW 2 cut(s) 267, 1287
TauI GCSGC 1 cut(s) 1799
TfiI GAWTC 7 cut(s) 422, 476, 875, 880, 1001, 1238, 1644
Tru1I TTAA 2 cut(s) 762, 1514
Tru9I TTAA 2 cut(s) 762, 1514
TscAI CASTG 5 cut(s) 67, 535, 1060, 1386, 1678
TseFI GTSAC 3 cut(s) 511, 574, 1682
TseI GCWGC 4 cut(s) 129, 241, 534, 1402
Tsp45I GTSAC 3 cut(s) 511, 574, 1682
TspDTI ATGAA 7 cut(s) 17, 339, 699, 918, 985, 1323, 1616
TspGWI ACGGA 4 cut(s) 410, 1239, 1513, 1730
TspRI CASTG 5 cut(s) 67, 535, 1060, 1386, 1678
VneI GTGCAC 2 cut(s) 340, 1476
VpaK11BI GGWCC 2 cut(s) 766, 1247
XapI RAATTY 4 cut(s) 15, 433, 1413, 1506
XceI RCATGY 3 cut(s) 898, 962, 1288
XmiI GTMKAC 2 cut(s) 1221, 1253
XmnI GAANNNNTTC 1 cut(s) 1648
XspI CTAG 4 cut(s) 698, 888, 1298, 1677
ZrmI AGTACT 1 cut(s) 269
Zsp2I ATGCAT 2 cut(s) 960, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.