RH5CG149700

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000360
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG149700.1

Sequence Viewer

Length: 1656 bp
ATGCCAGAACCCGAGTACCCTCCTGAGCCACCTACCTTCTCTGACGATGAACCTCTCCCATCGTGCACCTCATCCATTCAATCCTCTGCCCCGCAGAATATAACAGTCAAGACTCATACAGAGTCATCTGCTATCTCTTCTGCAGAATCAATCCCAAATTTTCCTGTCCTTGTCAGCATTCTTGCACCACCCCTTCAGGATTCTGAGGGCCATGGTCGTACGCCCATTGACCTTGTGACAGTTCTGGATGTCAGTGGCAGCATGGAAGGCACAAAGCTTTCCCTACTCAAGCAAGCTGTCACATTTGTCATACAAAACTTGGGGCCTTCGGATAGACTTTCTGTAGTTTCATTCTCATCAAGTTGTAGAAGAGTCTTTCCTCTCCAGAGAATGTCTGTTGATGGCCATGAAAGTGCCATCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCTGAAGGACTCAAGAAAGGAATTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGTATCATCCTCTTATCTGATGGCATAGATACATACAATCGAACTGCAAACAGACTGTTGAACCAGTTGCCTGATTCGATTCGTTCTAGCAACATGCAACATGAAATCCCAGTCCACACATTTGGATTTGGCTACGACCATGATCCCAACATTATGCATGCTATATCTGATCTGTCAGGTGGCACCTTTTCATTCATTGAATCAGTTGGAATGATACAAGACTCCTTTGCTCTGTGTATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCGCCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACACGTGAATGAGATTTCTGATGAGGGTCAACAAGGTGTTGTTCACGTTGGAAATATGTACGCAGAAGAGGAGAAACAATTTCTGGTCTACCTCTTAGTTCCAGAATCCTCAGGTCCGTATACTAAGATGTCATTGTTAGATGTGTTATGCATGTACAAAGATCTAGCTTCCAATGAACTCATCCGGGTGCAAGGTGAGAAAATGGAGATACTGAGACCCGAGGTTTGTTCCCCTGCAGAAAAGGCAGTGTCTTTGGAGGTTGATCGACAGCGGAACAGAATTGTGGTGGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGATGGGAAACCTGGAAGGTGCACGGGCACTTTTGGCTGAACGAAGAGCAACTGTCTTAGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCTCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGCTAGGGGTGACACAATGACCTCATGTCTCTTTGCTGCCTCCAACCGTGTGAATTTCTCCAGCGGTGGCAATGCCTTAATGCAGCAGTCAGGTGCCTCTGCTTGTTTTGGGGCCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCCAGTGCCGCTCCAGGTGGAAGTCAATGCTCTTCTGCAGTTGGTGCTTTTGAAACACCTGCTATGGTTAGAATGGTAGAAAAATCACAGAAGAGCAATCAATCTGGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

551

Amino Acids

58.78

Weight (kDa)

5.25

Isoelectric Point (pI)

60.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_CoxE PF05762 73 - 198 1.3e-08 VWA domain containing CoxE-like protein
VWA PF00092 77 - 242 9.5e-22 von Willebrand factor type A domain
VWA_3 PF13768 77 - 240 2.9e-18 von Willebrand factor type A domain
VWA_2 PF13519 78 - 183 1e-18 von Willebrand factor type A domain
WAV3_C PF25243 373 - 439 5.8e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 429 - 541 2.8e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 434, 1609
Acc36I ACCTGC 3 cut(s) 434, 1508, 1609
AccB1I GGYRCC 2 cut(s) 707, 1490
AccBSI CCGCTC 1 cut(s) 1553
AccI GTMKAC 2 cut(s) 945, 977
AciI CCGC 4 cut(s) 92, 1129, 1461, 1551
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 157, 1450
AcuI CTGAAG 3 cut(s) 179, 486, 1380
AcvI CACGTG 1 cut(s) 862
AfaI GTAC 5 cut(s) 17, 220, 917, 1013, 1334
AflIII ACRYGT 1 cut(s) 859
AgsI TTSAA 6 cut(s) 80, 497, 586, 725, 1286, 1595
AhdI GACNNNNNGTC 1 cut(s) 1422
AjnI CCWGG 3 cut(s) 823, 1191, 1555
AluBI AGCT 7 cut(s) 277, 296, 1025, 1253, 1259, 1294, 1400
AluI AGCT 7 cut(s) 277, 296, 1025, 1253, 1259, 1294, 1400
Alw21I GWGCWC 2 cut(s) 68, 1204
Alw26I GTCTC 5 cut(s) 438, 850, 1066, 1274, 1430
Alw44I GTGCAC 2 cut(s) 64, 1200
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 2 cut(s) 11, 1076
AoxI GGCC 4 cut(s) 208, 323, 403, 1509
ApaI GGGCCC 1 cut(s) 1513
ApaLI GTGCAC 2 cut(s) 64, 1200
ApeKI GCWGC 5 cut(s) 258, 1247, 1250, 1433, 1480
ApoI RAATTY 2 cut(s) 157, 1450
ArsI GACNNNNNNTTYG 2 cut(s) 1065, 1097
AspS9I GGNCC 6 cut(s) 208, 323, 490, 971, 1509, 1510
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 3 cut(s) 841, 1064, 1418
AvaI CYCGRG 2 cut(s) 11, 1076
AvaII GGWCC 2 cut(s) 490, 971
AxyI CCTNAGG 1 cut(s) 967
BaeGI GKGCMC 4 cut(s) 68, 1204, 1210, 1513
BaeI ACNNNNGTAYC 1 cut(s) 32
BalI TGGCCA 1 cut(s) 405
BanI GGYRCC 2 cut(s) 707, 1490
BanII GRGCYC 1 cut(s) 1513
BbrPI CACGTG 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 764
Bbv12I GWGCWC 2 cut(s) 68, 1204
BbvI GCAGC 5 cut(s) 270, 1259, 1262, 1420, 1492
BccI CCATC 7 cut(s) 67, 395, 425, 539, 856, 1177, 1259
BciT130I CCWGG 3 cut(s) 825, 1193, 1557
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 5 cut(s) 438, 850, 1066, 1274, 1430
BfaI CTAG 5 cut(s) 422, 612, 1022, 1401, 1533
BfmI CTRYAG 5 cut(s) 141, 342, 1092, 1515, 1578
BfuAI ACCTGC 3 cut(s) 434, 1508, 1609
BglII AGATCT 1 cut(s) 1018
BisI GCNGC 6 cut(s) 259, 1248, 1251, 1434, 1481, 1551
BlsI GCNGC 6 cut(s) 260, 1249, 1252, 1435, 1482, 1552
Bme1390I CCNGG 4 cut(s) 825, 1043, 1193, 1557
Bme18I GGWCC 2 cut(s) 490, 971
BmeRI GACNNNNNGTC 1 cut(s) 1422
BmeT110I CYCGRG 2 cut(s) 11, 1076
BmgT120I GGNCC 6 cut(s) 208, 323, 490, 971, 1509, 1510
BmiI GGNNCC 6 cut(s) 324, 709, 1492, 1510, 1511, 1512
BmrFI CCNGG 4 cut(s) 825, 1043, 1193, 1557
BmrI ACTGGG 2 cut(s) 512, 629
BmsI GCATC 3 cut(s) 827, 1250, 1328
BmuI ACTGGG 2 cut(s) 512, 629
BpiI GAAGAC 1 cut(s) 764
BpmI CTGGAG 3 cut(s) 368, 1441, 1539
Bpu10I CCTNAGC 2 cut(s) 24, 789
BpuEI CTTGAG 3 cut(s) 272, 458, 1239
BpuMI CCSGG 1 cut(s) 1043
BsaAI YACGTR 1 cut(s) 862
BsaI GGTCTC 2 cut(s) 1066, 1274
BsaJI CCNNGG 3 cut(s) 211, 824, 1077
Bse1I ACTGG 5 cut(s) 518, 525, 589, 635, 1545
Bse21I CCTNAGG 1 cut(s) 967
Bse3DI GCAATG 2 cut(s) 459, 1474
BseBI CCWGG 3 cut(s) 825, 1193, 1557
BseDI CCNNGG 3 cut(s) 211, 824, 1077
BseGI GGATG 8 cut(s) 71, 253, 417, 531, 1038, 1270, 1374, 1541
BseMI GCAATG 2 cut(s) 459, 1474
BseNI ACTGG 5 cut(s) 518, 525, 589, 635, 1545
BseRI GAGGAG 1 cut(s) 941
BseSI GKGCMC 4 cut(s) 68, 1204, 1210, 1513
BseXI GCAGC 5 cut(s) 270, 1259, 1262, 1420, 1492
BseYI CCCAGC 1 cut(s) 1259
BshFI GGCC 4 cut(s) 210, 325, 405, 1511
BshNI GGYRCC 2 cut(s) 707, 1490
BsiHKAI GWGCWC 2 cut(s) 68, 1204
BsiHKCI CYCGRG 2 cut(s) 11, 1076
BsiSI CCGG 1 cut(s) 1042
BsiWI CGTACG 1 cut(s) 218
BsmAI GTCTC 5 cut(s) 438, 850, 1066, 1274, 1430
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 4 cut(s) 210, 325, 405, 1511
Bso31I GGTCTC 2 cut(s) 1066, 1274
BsoBI CYCGRG 2 cut(s) 11, 1076
Bsp120I GGGCCC 1 cut(s) 1509
Bsp1286I GDGCHC 4 cut(s) 68, 1204, 1210, 1513
Bsp1407I TGTACA 1 cut(s) 1011
Bsp143I GATC 5 cut(s) 499, 667, 694, 1018, 1120
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 4 cut(s) 92, 1129, 1461, 1551
BspANI GGCC 4 cut(s) 210, 325, 405, 1511
BspLI GGNNCC 6 cut(s) 324, 709, 1492, 1510, 1511, 1512
BspMAI CTGCAG 4 cut(s) 145, 1096, 1519, 1582
BspMI ACCTGC 3 cut(s) 434, 1508, 1609
BspPI GGATC 1 cut(s) 662
BspQI GCTCTTC 3 cut(s) 1219, 1579, 1628
BspT107I GGYRCC 2 cut(s) 707, 1490
BspTNI GGTCTC 2 cut(s) 1066, 1274
BsrBI CCGCTC 1 cut(s) 1553
BsrDI GCAATG 2 cut(s) 459, 1474
BsrGI TGTACA 1 cut(s) 1011
BsrI ACTGG 5 cut(s) 518, 525, 589, 635, 1545
BssECI CCNNGG 3 cut(s) 211, 824, 1077
BssMI GATC 5 cut(s) 499, 667, 694, 1018, 1120
BssNAI GTATAC 1 cut(s) 978
BssT1I CCWWGG 1 cut(s) 211
Bst1107I GTATAC 1 cut(s) 978
Bst2UI CCWGG 3 cut(s) 825, 1193, 1557
Bst4CI ACNGT 6 cut(s) 106, 241, 582, 808, 1234, 1445
Bst6I CTCTTC 6 cut(s) 142, 364, 918, 1219, 1579, 1628
BstAPI GCANNNNNTGC 2 cut(s) 449, 1586
BstAUI TGTACA 1 cut(s) 1011
BstBAI YACGTR 1 cut(s) 862
BstC8I GCNNGC 3 cut(s) 294, 684, 1350
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 8 cut(s) 71, 253, 417, 531, 1038, 1270, 1374, 1541
BstKTI GATC 5 cut(s) 502, 670, 697, 1021, 1123
BstMAI GTCTC 5 cut(s) 438, 850, 1066, 1274, 1430
BstMBI GATC 5 cut(s) 499, 667, 694, 1018, 1120
BstMWI GCNNNNNNNGC 8 cut(s) 267, 422, 449, 1100, 1214, 1247, 1256, 1586
BstNI CCWGG 3 cut(s) 825, 1193, 1557
BstNSI RCATGY 3 cut(s) 622, 686, 1012
BstSCI CCNGG 4 cut(s) 823, 1041, 1191, 1555
BstSFI CTRYAG 5 cut(s) 141, 342, 1092, 1515, 1578
BstSLI GKGCMC 4 cut(s) 68, 1204, 1210, 1513
BstV1I GCAGC 5 cut(s) 270, 1259, 1262, 1420, 1492
BstV2I GAAGAC 1 cut(s) 764
BstX2I RGATCY 1 cut(s) 1018
BstXI CCANNNNNNTGG 1 cut(s) 647
BstYI RGATCY 1 cut(s) 1018
BstZ17I GTATAC 1 cut(s) 978
Bsu36I CCTNAGG 1 cut(s) 967
BsuRI GGCC 4 cut(s) 210, 325, 405, 1511
BtgI CCRYGG 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 804
BtsCI GGATG 8 cut(s) 71, 253, 417, 531, 1038, 1270, 1374, 1541
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 4 cut(s) 259, 784, 1110, 1552
BveI ACCTGC 3 cut(s) 434, 1508, 1609
Cac8I GCNNGC 3 cut(s) 294, 684, 1350
Cfr13I GGNCC 6 cut(s) 208, 323, 490, 971, 1509, 1510
Csp6I GTAC 5 cut(s) 16, 219, 916, 1012, 1333
CviQI GTAC 5 cut(s) 16, 219, 916, 1012, 1333
DpnI GATC 5 cut(s) 501, 669, 696, 1020, 1122
DpnII GATC 5 cut(s) 499, 667, 694, 1018, 1120
DriI GACNNNNNGTC 1 cut(s) 1422
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 6 cut(s) 142, 364, 918, 1219, 1579, 1628
Eam1105I GACNNNNNGTC 1 cut(s) 1422
EarI CTCTTC 6 cut(s) 142, 364, 918, 1219, 1579, 1628
Eco130I CCWWGG 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 1513
Eco31I GGTCTC 2 cut(s) 1066, 1274
Eco47I GGWCC 2 cut(s) 490, 971
Eco57I CTGAAG 3 cut(s) 179, 486, 1380
Eco72I CACGTG 1 cut(s) 862
Eco81I CCTNAGG 1 cut(s) 967
Eco88I CYCGRG 2 cut(s) 11, 1076
EcoO109I RGGNCCY 4 cut(s) 323, 490, 1509, 1510
EcoRII CCWGG 3 cut(s) 823, 1191, 1555
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 2 cut(s) 684, 1010
EcoT38I GRGCYC 1 cut(s) 1513
ErhI CCWWGG 1 cut(s) 211
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 2 cut(s) 945, 977
Fnu4HI GCNGC 6 cut(s) 259, 1248, 1251, 1434, 1481, 1551
FokI GGATG 8 cut(s) 58, 260, 404, 518, 1025, 1277, 1381, 1528
FriOI GRGCYC 1 cut(s) 1513
Fsp4HI GCNGC 6 cut(s) 259, 1248, 1251, 1434, 1481, 1551
FspBI CTAG 5 cut(s) 422, 612, 1022, 1401, 1533
GluI GCNGC 6 cut(s) 259, 1248, 1251, 1434, 1481, 1551
GsaI CCCAGC 1 cut(s) 1263
GsuI CTGGAG 3 cut(s) 368, 1441, 1539
HaeIII GGCC 4 cut(s) 210, 325, 405, 1511
HapII CCGG 1 cut(s) 1042
HincII GTYRAC 1 cut(s) 887
HindII GTYRAC 1 cut(s) 887
HindIII AAGCTT 1 cut(s) 275
HpaII CCGG 1 cut(s) 1042
HphI GGTGA 3 cut(s) 841, 1064, 1418
Hpy166II GTNNAC 7 cut(s) 66, 640, 887, 901, 946, 978, 1202
Hpy188I TCNGA 6 cut(s) 43, 205, 331, 544, 694, 877
Hpy188III TCNNGA 9 cut(s) 23, 109, 197, 245, 385, 475, 791, 959, 1383
Hpy8I GTNNAC 7 cut(s) 66, 640, 887, 901, 946, 978, 1202
HpyAV CCTTC 7 cut(s) 46, 203, 260, 336, 461, 498, 1190
HpyCH4III ACNGT 6 cut(s) 106, 241, 582, 808, 1234, 1445
HpyCH4IV ACGT 2 cut(s) 861, 903
HpyF10VI GCNNNNNNNGC 8 cut(s) 267, 422, 449, 1100, 1214, 1247, 1256, 1586
HpySE526I ACGT 2 cut(s) 861, 903
Kzo9I GATC 5 cut(s) 499, 667, 694, 1018, 1120
LguI GCTCTTC 3 cut(s) 1219, 1579, 1628
LmnI GCTCC 1 cut(s) 1558
Lsp1109I GCAGC 5 cut(s) 270, 1259, 1262, 1420, 1492
LweI GCATC 3 cut(s) 827, 1250, 1328
MaeI CTAG 5 cut(s) 422, 612, 1022, 1401, 1533
MaeII ACGT 2 cut(s) 861, 903
MaeIII GTNAC 3 cut(s) 235, 298, 1406
MalI GATC 5 cut(s) 501, 669, 696, 1020, 1122
MbiI CCGCTC 1 cut(s) 1553
MboI GATC 5 cut(s) 499, 667, 694, 1018, 1120
MboII GAAGA 9 cut(s) 129, 381, 509, 764, 844, 935, 1236, 1566, 1645
MflI RGATCY 1 cut(s) 1018
MhlI GDGCHC 4 cut(s) 68, 1204, 1210, 1513
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 6 cut(s) 157, 483, 935, 1137, 1276, 1450
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 6 cut(s) 106, 131, 381, 440, 465, 740
MmeI TCCRAC 3 cut(s) 712, 886, 1464
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 2 cut(s) 684, 1010
MscI TGGCCA 1 cut(s) 405
MseI TTAA 2 cut(s) 486, 1475
MslI CAYNNNNRTG 3 cut(s) 411, 864, 1043
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 2 cut(s) 1129, 1461
MspI CCGG 1 cut(s) 1042
MspR9I CCNGG 4 cut(s) 825, 1043, 1193, 1557
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 3 cut(s) 825, 1193, 1557
MwoI GCNNNNNNNGC 8 cut(s) 267, 422, 449, 1100, 1214, 1247, 1256, 1586
NciI CCSGG 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 211
NdeII GATC 5 cut(s) 499, 667, 694, 1018, 1120
NlaIV GGNNCC 6 cut(s) 324, 709, 1492, 1510, 1511, 1512
NmuCI GTSAC 3 cut(s) 235, 298, 1406
NsiI ATGCAT 2 cut(s) 684, 1010
NspI RCATGY 3 cut(s) 622, 686, 1012
PaeI GCATGC 1 cut(s) 686
PaqCI CACCTGC 2 cut(s) 434, 1609
PciSI GCTCTTC 3 cut(s) 1219, 1579, 1628
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 6 cut(s) 146, 200, 599, 604, 725, 962
Pfl23II CGTACG 1 cut(s) 218
PkrI GCNGC 6 cut(s) 260, 1249, 1252, 1435, 1482, 1552
PleI GAGTC 6 cut(s) 106, 130, 380, 439, 465, 740
PmaCI CACGTG 1 cut(s) 862
PmlI CACGTG 1 cut(s) 862
PpsI GAGTC 6 cut(s) 106, 130, 380, 439, 465, 740
Ppu21I YACGTR 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 490
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 3 cut(s) 823, 1191, 1555
PspCI CACGTG 1 cut(s) 862
PspFI CCCAGC 1 cut(s) 1259
PspGI CCWGG 3 cut(s) 823, 1191, 1555
PspLI CGTACG 1 cut(s) 218
PspN4I GGNNCC 6 cut(s) 324, 709, 1492, 1510, 1511, 1512
PspOMI GGGCCC 1 cut(s) 1509
PspPI GGNCC 6 cut(s) 208, 323, 490, 971, 1509, 1510
PspPPI RGGWCCY 1 cut(s) 490
PstI CTGCAG 4 cut(s) 145, 1096, 1519, 1582
PsuI RGATCY 1 cut(s) 1018
RsaI GTAC 5 cut(s) 17, 220, 917, 1013, 1334
RsaNI GTAC 5 cut(s) 16, 219, 916, 1012, 1333
RseI CAYNNNNRTG 3 cut(s) 411, 864, 1043
SapI GCTCTTC 3 cut(s) 1219, 1579, 1628
SaqAI TTAA 2 cut(s) 486, 1475
SatI GCNGC 6 cut(s) 259, 1248, 1251, 1434, 1481, 1551
Sau3AI GATC 5 cut(s) 499, 667, 694, 1018, 1120
Sau96I GGNCC 6 cut(s) 208, 323, 490, 971, 1509, 1510
SbfI CCTGCAGG 1 cut(s) 1519
SchI GAGTC 6 cut(s) 106, 131, 381, 440, 465, 740
ScrFI CCNGG 4 cut(s) 825, 1043, 1193, 1557
SdaI CCTGCAGG 1 cut(s) 1519
SduI GDGCHC 4 cut(s) 68, 1204, 1210, 1513
SfaNI GCATC 3 cut(s) 827, 1250, 1328
SfcI CTRYAG 5 cut(s) 141, 342, 1092, 1515, 1578
SinI GGWCC 2 cut(s) 490, 971
SmiMI CAYNNNNRTG 3 cut(s) 411, 864, 1043
SmlI CTYRAG 3 cut(s) 287, 473, 1254
SmoI CTYRAG 3 cut(s) 287, 473, 1254
SphI GCATGC 1 cut(s) 686
Sse8387I CCTGCAGG 1 cut(s) 1519
Sse9I AATT 6 cut(s) 157, 483, 935, 1137, 1276, 1450
SsiI CCGC 4 cut(s) 92, 1129, 1461, 1551
SspMI CTAG 5 cut(s) 422, 612, 1022, 1401, 1533
StyD4I CCNGG 4 cut(s) 823, 1041, 1191, 1555
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 6 cut(s) 106, 241, 582, 808, 1234, 1445
TaiI ACGT 2 cut(s) 864, 906
TaqI TCGA 5 cut(s) 502, 565, 602, 1123, 1384
TasI AATT 6 cut(s) 157, 483, 935, 1137, 1276, 1450
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 1 cut(s) 1553
TfiI GAWTC 6 cut(s) 146, 200, 599, 604, 725, 962
Tru1I TTAA 2 cut(s) 486, 1475
Tru9I TTAA 2 cut(s) 486, 1475
TscAI CASTG 4 cut(s) 259, 784, 1110, 1552
TseFI GTSAC 3 cut(s) 235, 298, 1406
TseI GCWGC 5 cut(s) 258, 1247, 1250, 1433, 1480
Tsp45I GTSAC 3 cut(s) 235, 298, 1406
TspDTI ATGAA 8 cut(s) 63, 339, 423, 642, 705, 709, 1047, 1530
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 4 cut(s) 259, 784, 1110, 1552
VneI GTGCAC 2 cut(s) 64, 1200
VpaK11BI GGWCC 2 cut(s) 490, 971
XapI RAATTY 2 cut(s) 157, 1450
XceI RCATGY 3 cut(s) 622, 686, 1012
XmiI GTMKAC 2 cut(s) 945, 977
XspI CTAG 5 cut(s) 422, 612, 1022, 1401, 1533
Zsp2I ATGCAT 2 cut(s) 684, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.