VWA / Hh protein intein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2705 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1311 |
| AatII | GACGTC | 2 cut(s) | 1430, 3273 |
| Acc16I | TGCGCA | 2 cut(s) | 2107, 2455 |
| Acc36I | ACCTGC | 3 cut(s) | 1871, 1901, 2705 |
| AccB1I | GGYRCC | 1 cut(s) | 2978 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 291 |
| AccI | GTMKAC | 1 cut(s) | 1437 |
| AccII | CGCG | 1 cut(s) | 3759 |
| AccIII | TCCGGA | 1 cut(s) | 1414 |
| AclI | AACGTT | 1 cut(s) | 1157 |
| AclWI | GGATC | 5 cut(s) | 162, 641, 2465, 2478, 3840 |
| AcoI | YGGCCR | 7 cut(s) | 263, 715, 994, 1869, 2674, 2818, 3699 |
| AcsI | RAATTY | 9 cut(s) | 35, 82, 624, 749, 1768, 2573, 2632, 3460, 3480 |
| AcuI | CTGAAG | 4 cut(s) | 2062, 2450, 2583, 3228 |
| AcvI | CACGTG | 1 cut(s) | 3476 |
| AcyI | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| AdeI | CACNNNGTG | 1 cut(s) | 3841 |
| AfaI | GTAC | 9 cut(s) | 667, 1166, 1388, 1454, 2491, 3188, 3284, 3605, 3969 |
| AflIII | ACRYGT | 1 cut(s) | 1306 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 784 |
| AjnI | CCWGG | 3 cut(s) | 1270, 1866, 3696 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1565, 1597, 3395, 3427 |
| Alw21I | GWGCWC | 5 cut(s) | 344, 634, 2168, 2305, 3475 |
| Alw26I | GTCTC | 8 cut(s) | 563, 1297, 1507, 1999, 2387, 3242, 3545, 3849 |
| Alw44I | GTGCAC | 3 cut(s) | 630, 2301, 3471 |
| AlwI | GGATC | 5 cut(s) | 162, 641, 2465, 2478, 3840 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1381, 2451 |
| Ama87I | CYCGRG | 1 cut(s) | 2973 |
| Aor13HI | TCCGGA | 1 cut(s) | 1414 |
| ApaLI | GTGCAC | 3 cut(s) | 630, 2301, 3471 |
| ApoI | RAATTY | 9 cut(s) | 35, 82, 624, 749, 1768, 2573, 2632, 3460, 3480 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1506, 1538, 1977, 2009, 3336, 3368 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 65, 195, 1159, 3643 |
| AspLEI | GCGC | 2 cut(s) | 2108, 2456 |
| AspS9I | GGNCC | 8 cut(s) | 536, 850, 1486, 1833, 2360, 2539, 3663, 3806 |
| AsuC2I | CCSGG | 5 cut(s) | 1484, 2974, 2975, 3751, 3784 |
| AsuHPI | GGTGA | 8 cut(s) | 668, 1260, 1267, 1288, 1505, 2329, 2334, 3211 |
| AsuII | TTCGAA | 1 cut(s) | 2656 |
| AsuNHI | GCTAGC | 2 cut(s) | 971, 2795 |
| AvaI | CYCGRG | 1 cut(s) | 2973 |
| AvaII | GGWCC | 2 cut(s) | 1486, 3806 |
| BaeGI | GKGCMC | 5 cut(s) | 146, 634, 2305, 2981, 3475 |
| BalI | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| BamHI | GGATCC | 1 cut(s) | 2470 |
| BanI | GGYRCC | 1 cut(s) | 2978 |
| BauI | CACGAG | 2 cut(s) | 1861, 3691 |
| BbrPI | CACGTG | 1 cut(s) | 3476 |
| BbsI | GAAGAC | 3 cut(s) | 1211, 2589, 3035 |
| Bbv12I | GWGCWC | 5 cut(s) | 344, 634, 2168, 2305, 3475 |
| BbvCI | CCTCAGC | 1 cut(s) | 3238 |
| BceAI | ACGGC | 5 cut(s) | 648, 837, 1841, 2661, 3169 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 1097, 1131, 1842, 1876, 2921, 2955, 3672, 3706 |
| BciT130I | CCWGG | 3 cut(s) | 1272, 1868, 3698 |
| BciVI | GTATCC | 1 cut(s) | 1411 |
| BcnI | CCSGG | 5 cut(s) | 1484, 2974, 2975, 3751, 3784 |
| BcoDI | GTCTC | 8 cut(s) | 563, 1297, 1507, 1999, 2387, 3242, 3545, 3849 |
| BfmI | CTRYAG | 5 cut(s) | 409, 789, 1533, 2613, 3363 |
| BfuAI | ACCTGC | 3 cut(s) | 1871, 1901, 2705 |
| BfuI | GTATCC | 1 cut(s) | 1411 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1877 |
| BglII | AGATCT | 2 cut(s) | 1459, 3289 |
| BlpI | GCTNAGC | 1 cut(s) | 3883 |
| Bme1390I | CCNGG | 8 cut(s) | 1272, 1484, 1868, 2974, 2975, 3698, 3751, 3784 |
| Bme18I | GGWCC | 2 cut(s) | 1486, 3806 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 784 |
| BmeT110I | CYCGRG | 1 cut(s) | 2973 |
| BmgT120I | GGNCC | 8 cut(s) | 536, 850, 1486, 1833, 2360, 2539, 3663, 3806 |
| BmiI | GGNNCC | 7 cut(s) | 538, 962, 2362, 2472, 2786, 2980, 3808 |
| BmrFI | CCNGG | 8 cut(s) | 1272, 1484, 1868, 2974, 2975, 3698, 3751, 3784 |
| BmrI | ACTGGG | 2 cut(s) | 1076, 2783 |
| BmtI | GCTAGC | 2 cut(s) | 975, 2799 |
| BmuI | ACTGGG | 2 cut(s) | 1076, 2783 |
| BpiI | GAAGAC | 3 cut(s) | 1211, 2589, 3035 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1659, 1691, 3489, 3521 |
| BpmI | CTGGAG | 3 cut(s) | 313, 1655, 2137 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1236, 3060, 3238 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3883 |
| Bpu14I | TTCGAA | 1 cut(s) | 2656 |
| BpuEI | CTTGAG | 2 cut(s) | 905, 962 |
| BpuMI | CCSGG | 5 cut(s) | 1484, 2974, 2975, 3751, 3784 |
| BsaAI | YACGTR | 1 cut(s) | 3476 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3927 |
| BsaHI | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| BsaI | GGTCTC | 3 cut(s) | 563, 1507, 2387 |
| BsaJI | CCNNGG | 7 cut(s) | 1271, 1489, 1836, 2973, 2974, 3750, 3783 |
| BsaWI | WCCGGW | 1 cut(s) | 1414 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 266, 296, 1361, 1391, 1990, 2020 |
| Bse118I | RCCGGY | 1 cut(s) | 2344 |
| Bse1I | ACTGG | 3 cut(s) | 273, 1082, 2789 |
| Bse3DI | GCAATG | 8 cut(s) | 361, 366, 906, 2190, 2730, 3834, 3925, 3960 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3927 |
| BseAI | TCCGGA | 1 cut(s) | 1414 |
| BseBI | CCWGG | 3 cut(s) | 1272, 1868, 3698 |
| BseDI | CCNNGG | 7 cut(s) | 1271, 1489, 1836, 2973, 2974, 3750, 3783 |
| BseGI | GGATG | 6 cut(s) | 202, 978, 997, 1711, 1815, 2090 |
| BseJI | GATNNNNATC | 1 cut(s) | 3927 |
| BseMI | GCAATG | 8 cut(s) | 361, 366, 906, 2190, 2730, 3834, 3925, 3960 |
| BseNI | ACTGG | 3 cut(s) | 273, 1082, 2789 |
| BseRI | GAGGAG | 6 cut(s) | 512, 959, 1382, 1969, 1995, 2036 |
| BseSI | GKGCMC | 5 cut(s) | 146, 634, 2305, 2981, 3475 |
| BseYI | CCCAGC | 2 cut(s) | 1700, 3530 |
| BsgI | GTGCAG | 1 cut(s) | 1626 |
| Bsh1236I | CGCG | 1 cut(s) | 3759 |
| BshNI | GGYRCC | 1 cut(s) | 2978 |
| BsiHKAI | GWGCWC | 5 cut(s) | 344, 634, 2168, 2305, 3475 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2973 |
| BsiSI | CCGG | 9 cut(s) | 152, 262, 1415, 1484, 2070, 2345, 2974, 3750, 3783 |
| BslFI | GGGAC | 2 cut(s) | 784, 3792 |
| BsmAI | GTCTC | 8 cut(s) | 563, 1297, 1507, 1999, 2387, 3242, 3545, 3849 |
| BsmBI | CGTCTC | 1 cut(s) | 1999 |
| BsmFI | GGGAC | 2 cut(s) | 784, 3792 |
| Bso31I | GGTCTC | 3 cut(s) | 563, 1507, 2387 |
| BsoBI | CYCGRG | 1 cut(s) | 2973 |
| Bsp119I | TTCGAA | 1 cut(s) | 2656 |
| Bsp1286I | GDGCHC | 7 cut(s) | 146, 344, 634, 2168, 2305, 2981, 3475 |
| Bsp13I | TCCGGA | 1 cut(s) | 1414 |
| Bsp1407I | TGTACA | 2 cut(s) | 1452, 3282 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3883 |
| BspEI | TCCGGA | 1 cut(s) | 1414 |
| BspFNI | CGCG | 1 cut(s) | 3759 |
| BspHI | TCATGA | 1 cut(s) | 1677 |
| BspLI | GGNNCC | 7 cut(s) | 538, 962, 2362, 2472, 2786, 2980, 3808 |
| BspMAI | CTGCAG | 2 cut(s) | 413, 1537 |
| BspMI | ACCTGC | 3 cut(s) | 1871, 1901, 2705 |
| BspOI | GCTAGC | 2 cut(s) | 975, 2799 |
| BspPI | GGATC | 5 cut(s) | 162, 641, 2465, 2478, 3840 |
| BspT104I | TTCGAA | 1 cut(s) | 2656 |
| BspT107I | GGYRCC | 1 cut(s) | 2978 |
| BspTNI | GGTCTC | 3 cut(s) | 563, 1507, 2387 |
| BsrDI | GCAATG | 8 cut(s) | 361, 366, 906, 2190, 2730, 3834, 3925, 3960 |
| BsrFI | RCCGGY | 1 cut(s) | 2344 |
| BsrGI | TGTACA | 2 cut(s) | 1452, 3282 |
| BsrI | ACTGG | 3 cut(s) | 273, 1082, 2789 |
| BssAI | RCCGGY | 1 cut(s) | 2344 |
| BssECI | CCNNGG | 7 cut(s) | 1271, 1489, 1836, 2973, 2974, 3750, 3783 |
| BssNI | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| BssSI | CACGAG | 2 cut(s) | 1861, 3691 |
| BssT1I | CCWWGG | 1 cut(s) | 1489 |
| Bst2BI | CACGAG | 2 cut(s) | 1861, 3691 |
| Bst2UI | CCWGG | 3 cut(s) | 1272, 1868, 3698 |
| BstACI | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1916 |
| BstAUI | TGTACA | 2 cut(s) | 1452, 3282 |
| BstBAI | YACGTR | 1 cut(s) | 3476 |
| BstBI | TTCGAA | 1 cut(s) | 2656 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1840, 3670 |
| BstF5I | GGATG | 6 cut(s) | 202, 978, 997, 1711, 1815, 2090 |
| BstFNI | CGCG | 1 cut(s) | 3759 |
| BstHHI | GCGC | 2 cut(s) | 2108, 2456 |
| BstMAI | GTCTC | 8 cut(s) | 563, 1297, 1507, 1999, 2387, 3242, 3545, 3849 |
| BstNI | CCWGG | 3 cut(s) | 1272, 1868, 3698 |
| BstNSI | RCATGY | 6 cut(s) | 712, 1069, 1310, 2536, 2893, 2957 |
| BstSCI | CCNGG | 8 cut(s) | 1270, 1482, 1866, 2972, 2973, 3696, 3749, 3782 |
| BstSFI | CTRYAG | 5 cut(s) | 409, 789, 1533, 2613, 3363 |
| BstSLI | GKGCMC | 5 cut(s) | 146, 634, 2305, 2981, 3475 |
| BstUI | CGCG | 1 cut(s) | 3759 |
| BstV2I | GAAGAC | 3 cut(s) | 1211, 2589, 3035 |
| BstX2I | RGATCY | 5 cut(s) | 154, 646, 1459, 2470, 3289 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1094, 2918 |
| BstYI | RGATCY | 5 cut(s) | 154, 646, 1459, 2470, 3289 |
| BsuI | GTATCC | 1 cut(s) | 1411 |
| BtsCI | GGATG | 6 cut(s) | 202, 978, 997, 1711, 1815, 2090 |
| BtsI | GCAGTG | 5 cut(s) | 330, 1551, 2154, 3381, 3774 |
| BtsIMutI | CAGTG | 9 cut(s) | 266, 330, 1231, 1551, 2154, 3055, 3084, 3381, 3774 |
| BveI | ACCTGC | 3 cut(s) | 1871, 1901, 2705 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1381, 2451 |
| CciI | TCATGA | 1 cut(s) | 1677 |
| CfoI | GCGC | 2 cut(s) | 2108, 2456 |
| Cfr10I | RCCGGY | 1 cut(s) | 2344 |
| Cfr13I | GGNCC | 8 cut(s) | 536, 850, 1486, 1833, 2360, 2539, 3663, 3806 |
| Cfr9I | CCCGGG | 1 cut(s) | 2973 |
| CseI | GACGC | 3 cut(s) | 77, 1202, 3026 |
| Csp6I | GTAC | 9 cut(s) | 666, 1165, 1387, 1453, 2490, 3187, 3283, 3604, 3968 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 344, 379, 2168, 2203 |
| CviQI | GTAC | 9 cut(s) | 666, 1165, 1387, 1453, 2490, 3187, 3283, 3604, 3968 |
| DraIII | CACNNNGTG | 1 cut(s) | 3841 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1311 |
| DriI | GACNNNNNGTC | 1 cut(s) | 784 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1311 |
| EaeI | YGGCCR | 7 cut(s) | 263, 715, 994, 1869, 2674, 2818, 3699 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 784 |
| EciI | GGCGGA | 2 cut(s) | 502, 1998 |
| Eco130I | CCWWGG | 1 cut(s) | 1489 |
| Eco31I | GGTCTC | 3 cut(s) | 563, 1507, 2387 |
| Eco32I | GATATC | 1 cut(s) | 3339 |
| Eco47I | GGWCC | 2 cut(s) | 1486, 3806 |
| Eco57I | CTGAAG | 4 cut(s) | 2062, 2450, 2583, 3228 |
| Eco72I | CACGTG | 1 cut(s) | 3476 |
| Eco88I | CYCGRG | 1 cut(s) | 2973 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1840, 3670 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1833, 3663 |
| EcoRII | CCWGG | 3 cut(s) | 1270, 1866, 3696 |
| EcoRV | GATATC | 1 cut(s) | 3339 |
| EcoT14I | CCWWGG | 1 cut(s) | 1489 |
| EcoT22I | ATGCAT | 3 cut(s) | 714, 1131, 2955 |
| ErhI | CCWWGG | 1 cut(s) | 1489 |
| Esp3I | CGTCTC | 1 cut(s) | 1999 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 85, 117 |
| FaqI | GGGAC | 2 cut(s) | 784, 3792 |
| FauI | CCCGC | 4 cut(s) | 1942, 2286, 3607, 3730 |
| FauNDI | CATATG | 1 cut(s) | 1719 |
| FblI | GTMKAC | 1 cut(s) | 1437 |
| FokI | GGATG | 6 cut(s) | 189, 965, 1004, 1718, 1822, 2077 |
| FspI | TGCGCA | 2 cut(s) | 2107, 2455 |
| GlaI | GCGC | 2 cut(s) | 2107, 2455 |
| GsaI | CCCAGC | 2 cut(s) | 1704, 3534 |
| GsuI | CTGGAG | 3 cut(s) | 313, 1655, 2137 |
| HapII | CCGG | 9 cut(s) | 152, 262, 1415, 1484, 2070, 2345, 2974, 3750, 3783 |
| HgaI | GACGC | 3 cut(s) | 77, 1202, 3026 |
| HhaI | GCGC | 2 cut(s) | 2108, 2456 |
| Hin1I | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| Hin6I | GCGC | 2 cut(s) | 2106, 2454 |
| HinP1I | GCGC | 2 cut(s) | 2106, 2454 |
| HindIII | AAGCTT | 2 cut(s) | 722, 2546 |
| HpaII | CCGG | 9 cut(s) | 152, 262, 1415, 1484, 2070, 2345, 2974, 3750, 3783 |
| HphI | GGTGA | 8 cut(s) | 668, 1260, 1267, 1288, 1505, 2329, 2334, 3211 |
| HpyCH4IV | ACGT | 8 cut(s) | 696, 1157, 1427, 1440, 2124, 2520, 3270, 3475 |
| HpySE526I | ACGT | 8 cut(s) | 696, 1157, 1427, 1440, 2124, 2520, 3270, 3475 |
| Hsp92I | GRCGYC | 3 cut(s) | 1427, 3018, 3270 |
| HspAI | GCGC | 2 cut(s) | 2106, 2454 |
| Kpn2I | TCCGGA | 1 cut(s) | 1414 |
| LmnI | GCTCC | 7 cut(s) | 287, 605, 946, 1417, 2031, 3098, 3321 |
| MaeII | ACGT | 8 cut(s) | 696, 1157, 1427, 1440, 2124, 2520, 3270, 3475 |
| MfeI | CAATTG | 2 cut(s) | 186, 2688 |
| MflI | RGATCY | 5 cut(s) | 154, 646, 1459, 2470, 3289 |
| MhlI | GDGCHC | 7 cut(s) | 146, 344, 634, 2168, 2305, 2981, 3475 |
| MlsI | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| MluNI | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| MmeI | TCCRAC | 1 cut(s) | 1327 |
| Mox20I | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| Mph1103I | ATGCAT | 3 cut(s) | 714, 1131, 2955 |
| MroI | TCCGGA | 1 cut(s) | 1414 |
| MroXI | GAANNNNTTC | 4 cut(s) | 65, 195, 1159, 3643 |
| MscI | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| MseI | TTAA | 3 cut(s) | 846, 1737, 3567 |
| MslI | CAYNNNNRTG | 1 cut(s) | 3933 |
| Msp20I | TGGCCA | 4 cut(s) | 717, 996, 2820, 3701 |
| MspA1I | CMGCKG | 3 cut(s) | 1570, 3400, 3737 |
| MspI | CCGG | 9 cut(s) | 152, 262, 1415, 1484, 2070, 2345, 2974, 3750, 3783 |
| MspR9I | CCNGG | 8 cut(s) | 1272, 1484, 1868, 2974, 2975, 3698, 3751, 3784 |
| MunI | CAATTG | 2 cut(s) | 186, 2688 |
| MvaI | CCWGG | 3 cut(s) | 1272, 1868, 3698 |
| MvnI | CGCG | 1 cut(s) | 3759 |
| NciI | CCSGG | 5 cut(s) | 1484, 2974, 2975, 3751, 3784 |
| NdeI | CATATG | 1 cut(s) | 1719 |
| NheI | GCTAGC | 2 cut(s) | 971, 2795 |
| NlaIV | GGNNCC | 7 cut(s) | 538, 962, 2362, 2472, 2786, 2980, 3808 |
| NmeAIII | GCCGAG | 2 cut(s) | 1817, 2261 |
| NmuCI | GTSAC | 5 cut(s) | 1010, 1061, 2125, 2371, 3769 |
| NsbI | TGCGCA | 2 cut(s) | 2107, 2455 |
| NsiI | ATGCAT | 3 cut(s) | 714, 1131, 2955 |
| NspI | RCATGY | 6 cut(s) | 712, 1069, 1310, 2536, 2893, 2957 |
| NspV | TTCGAA | 1 cut(s) | 2656 |
| PaeI | GCATGC | 3 cut(s) | 712, 2536, 2957 |
| PagI | TCATGA | 1 cut(s) | 1677 |
| PaqCI | CACCTGC | 1 cut(s) | 2705 |
| PciI | ACATGT | 1 cut(s) | 1306 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1446, 3276 |
| PdmI | GAANNNNTTC | 4 cut(s) | 65, 195, 1159, 3643 |
| PfeI | GAWTC | 8 cut(s) | 593, 878, 1172, 2417, 2702, 2969, 2996, 3988 |
| PflFI | GACNNNGTC | 1 cut(s) | 2555 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 291 |
| PmaCI | CACGTG | 1 cut(s) | 3476 |
| PmlI | CACGTG | 1 cut(s) | 3476 |
| Ppu21I | YACGTR | 1 cut(s) | 3476 |
| PscI | ACATGT | 1 cut(s) | 1306 |
| Psp1406I | AACGTT | 1 cut(s) | 1157 |
| Psp6I | CCWGG | 3 cut(s) | 1270, 1866, 3696 |
| PspCI | CACGTG | 1 cut(s) | 3476 |
| PspFI | CCCAGC | 2 cut(s) | 1700, 3530 |
| PspGI | CCWGG | 3 cut(s) | 1270, 1866, 3696 |
| PspN4I | GGNNCC | 7 cut(s) | 538, 962, 2362, 2472, 2786, 2980, 3808 |
| PspPI | GGNCC | 8 cut(s) | 536, 850, 1486, 1833, 2360, 2539, 3663, 3806 |
| PstI | CTGCAG | 2 cut(s) | 413, 1537 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1381, 2451 |
| PsuI | RGATCY | 5 cut(s) | 154, 646, 1459, 2470, 3289 |
| PsyI | GACNNNGTC | 1 cut(s) | 2555 |
| RsaI | GTAC | 9 cut(s) | 667, 1166, 1388, 1454, 2491, 3188, 3284, 3605, 3969 |
| RsaNI | GTAC | 9 cut(s) | 666, 1165, 1387, 1453, 2490, 3187, 3283, 3604, 3968 |
| RseI | CAYNNNNRTG | 1 cut(s) | 3933 |
| SaqAI | TTAA | 3 cut(s) | 846, 1737, 3567 |
| Sau96I | GGNCC | 8 cut(s) | 536, 850, 1486, 1833, 2360, 2539, 3663, 3806 |
| ScrFI | CCNGG | 8 cut(s) | 1272, 1484, 1868, 2974, 2975, 3698, 3751, 3784 |
| SduI | GDGCHC | 7 cut(s) | 146, 344, 634, 2168, 2305, 2981, 3475 |
| SfcI | CTRYAG | 5 cut(s) | 409, 789, 1533, 2613, 3363 |
| SfuI | TTCGAA | 1 cut(s) | 2656 |
| SinI | GGWCC | 2 cut(s) | 1486, 3806 |
| SmaI | CCCGGG | 1 cut(s) | 2975 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 3933 |
| SmlI | CTYRAG | 2 cut(s) | 920, 941 |
| SmoI | CTYRAG | 2 cut(s) | 920, 941 |
| SphI | GCATGC | 3 cut(s) | 712, 2536, 2957 |
| StyD4I | CCNGG | 8 cut(s) | 1270, 1482, 1866, 2972, 2973, 3696, 3749, 3782 |
| StyI | CCWWGG | 1 cut(s) | 1489 |
| TaiI | ACGT | 8 cut(s) | 699, 1160, 1430, 1443, 2127, 2523, 3273, 3478 |
| TaqI | TCGA | 5 cut(s) | 876, 2656, 2700, 2836, 3931 |
| TatI | WGTACW | 2 cut(s) | 1452, 3282 |
| TauI | GCSGC | 1 cut(s) | 2014 |
| TfiI | GAWTC | 8 cut(s) | 593, 878, 1172, 2417, 2702, 2969, 2996, 3988 |
| Tru1I | TTAA | 3 cut(s) | 846, 1737, 3567 |
| Tru9I | TTAA | 3 cut(s) | 846, 1737, 3567 |
| TscAI | CASTG | 9 cut(s) | 273, 337, 1231, 1551, 2161, 3055, 3084, 3381, 3774 |
| TseFI | GTSAC | 5 cut(s) | 1010, 1061, 2125, 2371, 3769 |
| Tsp45I | GTSAC | 5 cut(s) | 1010, 1061, 2125, 2371, 3769 |
| TspGWI | ACGGA | 4 cut(s) | 195, 821, 2477, 3912 |
| TspMI | CCCGGG | 1 cut(s) | 2973 |
| TspRI | CASTG | 9 cut(s) | 273, 337, 1231, 1551, 2161, 3055, 3084, 3381, 3774 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2555 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 291 |
| VneI | GTGCAC | 3 cut(s) | 630, 2301, 3471 |
| VpaK11BI | GGWCC | 2 cut(s) | 1486, 3806 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1840, 3670 |
| XapI | RAATTY | 9 cut(s) | 35, 82, 624, 749, 1768, 2573, 2632, 3460, 3480 |
| XbaI | TCTAGA | 2 cut(s) | 2515, 2636 |
| XceI | RCATGY | 6 cut(s) | 712, 1069, 1310, 2536, 2893, 2957 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1429, 3320 |
| XmaI | CCCGGG | 1 cut(s) | 2973 |
| XmiI | GTMKAC | 1 cut(s) | 1437 |
| XmnI | GAANNNNTTC | 4 cut(s) | 65, 195, 1159, 3643 |
| ZraI | GACGTC | 2 cut(s) | 1428, 3271 |
| Zsp2I | ATGCAT | 3 cut(s) | 714, 1131, 2955 |