Rh5BG138200

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000360
Physical Location & Seq
Forward (+)
14217036 .. 14220299
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG138200.1

Sequence Viewer

Length: 1842 bp
ATGCCGAAGTTGTCACTATCCCTTCTTCTCCATCCTAATAAGCATCGACGCCAGGGTTCGGCCCCTCCAAACATGAAGTCCTCTGATGCCTCGTCTGAGGCTGCATCTGATGATCAATTTTACCGAGTCCCCCCTCCGCAACAAAACGACCTAGTTCGGAGGCTAGAGCCTTCTTGGTCAGGACCAGCTAAAGCCTTCGCTGACGATGAACCTCTCCCCTCCACCTCACCCACTCAAGCTTCTGGCTCCCAGCAGGTGACATTCAAGACTCATACAGAGACCTCTGCTATCTGTGCTGCCGAATCACGCCCACAATTTCCTGTTCTTGTCAGCATTCGGGCACCACCTCTTCAAGATCCTGATGGCGAAGGCCGTACACCGATTGACCTTGTTATAGTTCTGGACGTAAGTTGCAGCATGGAAGGAAAAAAACTTGAACTTGTCCAGCAAGCTGTCAAATTTGTGATAGAAAACTTGGGGTCTTCAGACAGACTCTCGATAGTTACATTCTCAACAACTGCTAGAAGAGTATTTCCCCTCAGAAGAATGTCTGTTGAAGGCCGTGGAGATGCCATCCGAGCCGTCGACTCTCTTAGAACAGATTACATGACCAATATTGCGGCAGGCCTCAAGATAGGAGCTCAGATACTTGAAGAACGAAGGGAAAAGAACTCAGTTGCTAGCATCATCCTCTTATCTGATGGCCAAGATAACTGTTCAAGCCAAAGCGAAATGTTGAATAATTTGCCTTCTTCTAGCGACATGCAACAGGAAATTCCAGTCCACACATTTGGGTTTGGCAATGACCATGATCCAAAAACCATGCATGCTATATCTGTTGCATCAAGGGGTACCTTTTCGTACATTGAATCCGTTGGCATGATACAAGATGGTTTTGCTTTGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTCACAGTAAGGTCAGCTTCAAGTGGGGTGAAGATTGTATCAATTCCATCGGGGAGACATGTGAATGAGATTTCGGATGAGGGTAAGCAAGGTGTTGTTAATGTAGGAAATATGTATGCAGAAGAGGTGAAACAATTTCTGGTGTATCTCTTAGTTCCACCATCCTCAGCTTCAGACACCAAGACATCATTGTTAGACGTGTCATGCGCATACAAAGATCTAGCTTCGGATGAGTTGATCCAGGTCCAAGGTGAAAGAGTAGAGATTTCGAGACCAGGGTCTTGTTCTTCTGCAGAATTGGCAGTGTCTTTGGAGGTTGATCGGCAGAGGAATAGACTTTTGGTGGTCGATGTTATTGGAGAGGCTCAGAGGTTGTTGGCTGAAATGGGAAATCTGGAGGGTGCACGCGAGGTTTTGACTCAACGAAGAGAAACTCTCTTAACATCAGCAGCAGCCCAAGCTGGGGATAATCTCAGCCATTCATTTGAAATTGAGCTCAGAGAAGTCATGGACAAGATGGGGAGTAGGGAGTTTTACGAAGACGCAGGGCGTGCTTACGCTTATGCAGGAATGAGTTCCCATTCATGGCAGAGGGCTTCAGCTAGGGGTAACGCTAGAGGCTCATGTCTCGCTGCGGGTTTTAAAGCTGCCAAGGGAGGCGCCAAGGGTCACCGTTTTGGTGCCAGGGGTTGTGGTGGAGGTGGAGGTGGTCGTGGTCGTGGTGGCGGTGGTCGTGGTGCCTCCGGGGGTCACACCAAAAGTAGGCTGTTAAATGATGCTTTGTTATACGGTGACGAAGACGAAGCTGATGAGTGTGCTGACGCTGTTGGTGCTTTTGAAACACCCGCCATGGTTAGAATGGTACAGATATCACAGAAGAGGAATCGATCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

613

Amino Acids

65.76

Weight (kDa)

5.98

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA PF00092 129 - 288 1.1e-23 von Willebrand factor type A domain
VWA_3 PF13768 129 - 286 1.1e-18 von Willebrand factor type A domain
VWA_2 PF13519 130 - 236 7.1e-17 von Willebrand factor type A domain
Vwaint PF14624 473 - 518 7e-09 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc16I TGCGCA 1 cut(s) 1153
Acc36I ACCTGC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 851
AccB1I GGYRCC 5 cut(s) 340, 851, 1604, 1625, 1682
AccI GTMKAC 1 cut(s) 585
AccII CGCG 1 cut(s) 1353
AciI CCGC 5 cut(s) 137, 620, 1580, 1671, 1791
AclWI GGATC 3 cut(s) 350, 806, 1177
AcoI YGGCCR 1 cut(s) 703
AcsI RAATTY 2 cut(s) 458, 774
AcuI CTGAAG 3 cut(s) 468, 1101, 1527
AcyI GRCGYC 2 cut(s) 49, 1605
AfaI GTAC 4 cut(s) 376, 853, 863, 1809
AfiI CCNNNNNNNGG 5 cut(s) 58, 1407, 1408, 1530, 1707
AflIII ACRYGT 2 cut(s) 1003, 1143
AjiI CACGTC 1 cut(s) 1144
AjnI CCWGG 4 cut(s) 51, 1185, 1219, 1628
AjuI GAANNNNNNNTTGG 2 cut(s) 1268, 1300
Alw21I GWGCWC 3 cut(s) 643, 1351, 1443
Alw26I GTCTC 4 cut(s) 272, 994, 1210, 1577
Alw44I GTGCAC 1 cut(s) 1347
AlwI GGATC 3 cut(s) 350, 806, 1177
AoxI GGCC 5 cut(s) 60, 370, 559, 625, 703
ApaLI GTGCAC 1 cut(s) 1347
ApeKI GCWGC 7 cut(s) 101, 296, 414, 1394, 1397, 1577, 1592
ApoI RAATTY 2 cut(s) 458, 774
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
Asp700I GAANNNNTTC 1 cut(s) 1519
Asp718I GGTACC 1 cut(s) 851
AspLEI GCGC 2 cut(s) 1154, 1607
AspS9I GGNCC 3 cut(s) 61, 182, 1189
AsuC2I CCSGG 1 cut(s) 1690
AsuHPI GGTGA 7 cut(s) 219, 268, 985, 1084, 1208, 1607, 1748
AsuNHI GCTAGC 1 cut(s) 680
AvaII GGWCC 2 cut(s) 182, 1189
BaeGI GKGCMC 2 cut(s) 343, 1351
BalI TGGCCA 1 cut(s) 705
BanI GGYRCC 5 cut(s) 340, 851, 1604, 1625, 1682
BanII GRGCYC 2 cut(s) 643, 1443
BbsI GAAGAC 4 cut(s) 474, 908, 1491, 1749
Bbv12I GWGCWC 3 cut(s) 643, 1351, 1443
BbvCI CCTCAGC 1 cut(s) 1111
BbvI GCAGC 7 cut(s) 88, 283, 426, 1406, 1409, 1564, 1579
BccI CCATC 8 cut(s) 39, 356, 581, 695, 884, 1000, 1114, 1456
BceAI ACGGC 3 cut(s) 357, 546, 566
BciT130I CCWGG 4 cut(s) 53, 1187, 1221, 1630
BclI TGATCA 1 cut(s) 112
BcnI CCSGG 1 cut(s) 1690
BcoDI GTCTC 4 cut(s) 272, 994, 1210, 1577
BfaI CTAG 8 cut(s) 152, 164, 522, 681, 756, 1166, 1548, 1560
BfmI CTRYAG 1 cut(s) 1236
BfoI RGCGCY 1 cut(s) 1608
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 1162
BisI GCNGC 8 cut(s) 102, 297, 415, 621, 1395, 1398, 1578, 1593
BlsI GCNGC 8 cut(s) 103, 298, 416, 622, 1396, 1399, 1579, 1594
Bme1390I CCNGG 5 cut(s) 53, 1187, 1221, 1630, 1690
Bme18I GGWCC 2 cut(s) 182, 1189
BmgBI CACGTC 1 cut(s) 1144
BmgT120I GGNCC 3 cut(s) 61, 182, 1189
BmiI GGNNCC 7 cut(s) 63, 247, 342, 853, 1606, 1627, 1684
BmrFI CCNGG 5 cut(s) 53, 1187, 1221, 1630, 1690
BmsI GCATC 7 cut(s) 52, 76, 113, 559, 693, 851, 1711
BmtI GCTAGC 1 cut(s) 684
BoxI GACNNNNGTC 1 cut(s) 1222
BpiI GAAGAC 4 cut(s) 474, 908, 1491, 1749
BplI GAGNNNNNCTC 2 cut(s) 1365, 1397
BpmI CTGGAG 1 cut(s) 1361
Bpu10I CCTNAGC 2 cut(s) 933, 1111
BpuEI CTTGAG 2 cut(s) 219, 614
BpuMI CCSGG 1 cut(s) 1690
Bsa29I ATCGAT 1 cut(s) 1831
BsaHI GRCGYC 2 cut(s) 49, 1605
BsaI GGTCTC 2 cut(s) 272, 1210
BsaJI CCNNGG 9 cut(s) 52, 562, 1192, 1220, 1596, 1608, 1629, 1689, 1794
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 1407, 1408, 1530, 1707
Bse1I ACTGG 1 cut(s) 779
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 4 cut(s) 53, 1187, 1221, 1630
BseCI ATCGAT 1 cut(s) 1831
BseDI CCNNGG 9 cut(s) 52, 562, 1192, 1220, 1596, 1608, 1629, 1689, 1794
BseGI GGATG 6 cut(s) 31, 573, 687, 1027, 1106, 1180
BseLI CCNNNNNNNGG 5 cut(s) 58, 1407, 1408, 1530, 1707
BseMI GCAATG 1 cut(s) 808
BseNI ACTGG 1 cut(s) 779
BseSI GKGCMC 2 cut(s) 343, 1351
BseXI GCAGC 7 cut(s) 88, 283, 426, 1406, 1409, 1564, 1579
BseYI CCCAGC 2 cut(s) 249, 1406
Bsh1236I CGCG 1 cut(s) 1353
BshFI GGCC 5 cut(s) 62, 372, 561, 627, 705
BshNI GGYRCC 5 cut(s) 340, 851, 1604, 1625, 1682
BshVI ATCGAT 1 cut(s) 1831
BsiHKAI GWGCWC 3 cut(s) 643, 1351, 1443
BsiSI CCGG 1 cut(s) 1689
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 5 cut(s) 58, 1407, 1408, 1530, 1707
BsmAI GTCTC 4 cut(s) 272, 994, 1210, 1577
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 5 cut(s) 62, 372, 561, 627, 705
Bso31I GGTCTC 2 cut(s) 272, 1210
Bsp1286I GDGCHC 4 cut(s) 343, 643, 1351, 1443
Bsp143I GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
Bsp19I CCATGG 1 cut(s) 1794
BspACI CCGC 5 cut(s) 137, 620, 1580, 1671, 1791
BspANI GGCC 5 cut(s) 62, 372, 561, 627, 705
BspDI ATCGAT 1 cut(s) 1831
BspFNI CGCG 1 cut(s) 1353
BspLI GGNNCC 7 cut(s) 63, 247, 342, 853, 1606, 1627, 1684
BspMAI CTGCAG 1 cut(s) 1240
BspMI ACCTGC 1 cut(s) 244
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 3 cut(s) 350, 806, 1177
BspT107I GGYRCC 5 cut(s) 340, 851, 1604, 1625, 1682
BspTNI GGTCTC 2 cut(s) 272, 1210
BsrDI GCAATG 1 cut(s) 808
BsrI ACTGG 1 cut(s) 779
BssECI CCNNGG 9 cut(s) 52, 562, 1192, 1220, 1596, 1608, 1629, 1689, 1794
BssMI GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
BssNI GRCGYC 2 cut(s) 49, 1605
BssT1I CCWWGG 4 cut(s) 1192, 1596, 1608, 1794
Bst2UI CCWGG 4 cut(s) 53, 1187, 1221, 1630
Bst4CI ACNGT 4 cut(s) 716, 952, 1619, 1736
Bst6I CTCTTC 5 cut(s) 354, 520, 1062, 1366, 1817
BstACI GRCGYC 2 cut(s) 49, 1605
BstAPI GCANNNNNTGC 1 cut(s) 1496
BstC8I GCNNGC 6 cut(s) 450, 625, 682, 828, 1351, 1497
BstDSI CCRYGG 2 cut(s) 562, 1794
BstEII GGTNACC 1 cut(s) 1613
BstF5I GGATG 6 cut(s) 31, 573, 687, 1027, 1106, 1180
BstFNI CGCG 1 cut(s) 1353
BstH2I RGCGCY 1 cut(s) 1608
BstHHI GCGC 2 cut(s) 1154, 1607
BstKTI GATC 7 cut(s) 115, 358, 814, 1165, 1185, 1267, 1835
BstMAI GTCTC 4 cut(s) 272, 994, 1210, 1577
BstMBI GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
BstMWI GCNNNNNNNGC 6 cut(s) 293, 578, 1244, 1403, 1496, 1775
BstNI CCWGG 4 cut(s) 53, 1187, 1221, 1630
BstNSI RCATGY 3 cut(s) 766, 830, 1007
BstPAI GACNNNNGTC 1 cut(s) 1222
BstPI GGTNACC 1 cut(s) 1613
BstSCI CCNGG 5 cut(s) 51, 1185, 1219, 1628, 1688
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 2 cut(s) 343, 1351
BstUI CGCG 1 cut(s) 1353
BstV1I GCAGC 7 cut(s) 88, 283, 426, 1406, 1409, 1564, 1579
BstV2I GAAGAC 4 cut(s) 474, 908, 1491, 1749
BstX2I RGATCY 2 cut(s) 355, 1162
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 2 cut(s) 355, 1162
Bsu15I ATCGAT 1 cut(s) 1831
BsuRI GGCC 5 cut(s) 62, 372, 561, 627, 705
BsuTUI ATCGAT 1 cut(s) 1831
BtgI CCRYGG 2 cut(s) 562, 1794
BtrI CACGTC 1 cut(s) 1144
BtsCI GGATG 6 cut(s) 31, 573, 687, 1027, 1106, 1180
BtsI GCAGTG 1 cut(s) 1254
BtsIMutI CAGTG 2 cut(s) 928, 1254
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 6 cut(s) 450, 625, 682, 828, 1351, 1497
CfoI GCGC 2 cut(s) 1154, 1607
Cfr13I GGNCC 3 cut(s) 61, 182, 1189
ClaI ATCGAT 1 cut(s) 1831
CseI GACGC 3 cut(s) 57, 1496, 1775
Csp6I GTAC 4 cut(s) 375, 852, 862, 1808
CviQI GTAC 4 cut(s) 375, 852, 862, 1808
DinI GGCGCC 1 cut(s) 1606
DpnI GATC 7 cut(s) 114, 357, 813, 1164, 1184, 1266, 1834
DpnII GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
DraI TTTAAA 1 cut(s) 1588
EaeI YGGCCR 1 cut(s) 703
Eam1104I CTCTTC 5 cut(s) 354, 520, 1062, 1366, 1817
EarI CTCTTC 5 cut(s) 354, 520, 1062, 1366, 1817
Ecl136II GAGCTC 2 cut(s) 641, 1441
Eco130I CCWWGG 4 cut(s) 1192, 1596, 1608, 1794
Eco147I AGGCCT 1 cut(s) 627
Eco24I GRGCYC 2 cut(s) 643, 1443
Eco31I GGTCTC 2 cut(s) 272, 1210
Eco32I GATATC 1 cut(s) 1815
Eco47I GGWCC 2 cut(s) 182, 1189
Eco53kI GAGCTC 2 cut(s) 641, 1441
Eco57I CTGAAG 3 cut(s) 468, 1101, 1527
Eco91I GGTNACC 1 cut(s) 1613
EcoICRI GAGCTC 2 cut(s) 641, 1441
EcoO65I GGTNACC 1 cut(s) 1613
EcoRII CCWGG 4 cut(s) 51, 1185, 1219, 1628
EcoRV GATATC 1 cut(s) 1815
EcoT14I CCWWGG 4 cut(s) 1192, 1596, 1608, 1794
EcoT22I ATGCAT 1 cut(s) 828
EcoT38I GRGCYC 2 cut(s) 643, 1443
EgeI GGCGCC 1 cut(s) 1606
EheI GGCGCC 1 cut(s) 1606
ErhI CCWWGG 4 cut(s) 1192, 1596, 1608, 1794
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FaqI GGGAC 1 cut(s) 113
FauI CCCGC 2 cut(s) 1573, 1798
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 8 cut(s) 102, 297, 415, 621, 1395, 1398, 1578, 1593
FokI GGATG 6 cut(s) 18, 560, 674, 1034, 1093, 1187
FriOI GRGCYC 2 cut(s) 643, 1443
Fsp4HI GCNGC 8 cut(s) 102, 297, 415, 621, 1395, 1398, 1578, 1593
FspAI RTGCGCAY 1 cut(s) 1153
FspBI CTAG 8 cut(s) 152, 164, 522, 681, 756, 1166, 1548, 1560
FspI TGCGCA 1 cut(s) 1153
GlaI GCGC 2 cut(s) 1153, 1606
GluI GCNGC 8 cut(s) 102, 297, 415, 621, 1395, 1398, 1578, 1593
GsaI CCCAGC 2 cut(s) 253, 1410
GsuI CTGGAG 1 cut(s) 1361
HaeII RGCGCY 1 cut(s) 1608
HaeIII GGCC 5 cut(s) 62, 372, 561, 627, 705
HapII CCGG 1 cut(s) 1689
HgaI GACGC 3 cut(s) 57, 1496, 1775
HhaI GCGC 2 cut(s) 1154, 1607
Hin1I GRCGYC 2 cut(s) 49, 1605
Hin6I GCGC 2 cut(s) 1152, 1605
HinP1I GCGC 2 cut(s) 1152, 1605
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 8 cut(s) 126, 268, 302, 492, 587, 869, 1363, 1828
HpaII CCGG 1 cut(s) 1689
HphI GGTGA 7 cut(s) 219, 268, 985, 1084, 1208, 1607, 1748
Hpy166II GTNNAC 4 cut(s) 377, 586, 784, 1349
Hpy8I GTNNAC 4 cut(s) 377, 586, 784, 1349
Hpy99I CGWCG 2 cut(s) 51, 587
HpyAV CCTTC 8 cut(s) 32, 180, 205, 362, 416, 551, 654, 759
HpyCH4III ACNGT 4 cut(s) 716, 952, 1619, 1736
HpyCH4IV ACGT 2 cut(s) 405, 1143
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 578, 1244, 1403, 1496, 1775
HpySE526I ACGT 2 cut(s) 405, 1143
Hsp92I GRCGYC 2 cut(s) 49, 1605
HspAI GCGC 2 cut(s) 1152, 1605
KasI GGCGCC 1 cut(s) 1604
KpnI GGTACC 1 cut(s) 855
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
LmnI GCTCC 2 cut(s) 251, 638
Lsp1109I GCAGC 7 cut(s) 88, 283, 426, 1406, 1409, 1564, 1579
LweI GCATC 7 cut(s) 52, 76, 113, 559, 693, 851, 1711
MaeI CTAG 8 cut(s) 152, 164, 522, 681, 756, 1166, 1548, 1560
MaeII ACGT 2 cut(s) 405, 1143
MaeIII GTNAC 7 cut(s) 12, 256, 502, 1553, 1613, 1694, 1736
MalI GATC 7 cut(s) 114, 357, 813, 1164, 1184, 1266, 1834
MboI GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
MflI RGATCY 2 cut(s) 355, 1162
MhlI GDGCHC 4 cut(s) 343, 643, 1351, 1443
MlsI TGGCCA 1 cut(s) 705
MluNI TGGCCA 1 cut(s) 705
Mly113I GGCGCC 1 cut(s) 1605
MlyI GAGTC 5 cut(s) 135, 262, 486, 581, 1357
Mox20I TGGCCA 1 cut(s) 705
Mph1103I ATGCAT 1 cut(s) 828
MroXI GAANNNNTTC 1 cut(s) 1519
MscI TGGCCA 1 cut(s) 705
MseI TTAA 5 cut(s) 1044, 1385, 1587, 1715, 1840
MslI CAYNNNNRTG 1 cut(s) 1008
Msp20I TGGCCA 1 cut(s) 705
MspI CCGG 1 cut(s) 1689
MspR9I CCNGG 5 cut(s) 53, 1187, 1221, 1630, 1690
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 4 cut(s) 53, 1187, 1221, 1630
MvnI CGCG 1 cut(s) 1353
MwoI GCNNNNNNNGC 6 cut(s) 293, 578, 1244, 1403, 1496, 1775
NarI GGCGCC 1 cut(s) 1605
NciI CCSGG 1 cut(s) 1690
NcoI CCATGG 1 cut(s) 1794
NdeII GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
NheI GCTAGC 1 cut(s) 680
NlaIV GGNNCC 7 cut(s) 63, 247, 342, 853, 1606, 1627, 1684
NmuCI GTSAC 5 cut(s) 12, 256, 1613, 1694, 1736
NsbI TGCGCA 1 cut(s) 1153
NsiI ATGCAT 1 cut(s) 828
NspI RCATGY 3 cut(s) 766, 830, 1007
PaeI GCATGC 1 cut(s) 830
PaqCI CACCTGC 1 cut(s) 244
PceI AGGCCT 1 cut(s) 627
PciI ACATGT 1 cut(s) 1003
PctI GAATGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 1519
PfeI GAWTC 3 cut(s) 302, 869, 1828
PkrI GCNGC 8 cut(s) 103, 298, 416, 622, 1396, 1399, 1579, 1594
PleI GAGTC 5 cut(s) 134, 262, 486, 581, 1357
PluTI GGCGCC 1 cut(s) 1608
PpsI GAGTC 5 cut(s) 134, 262, 486, 581, 1357
PscI ACATGT 1 cut(s) 1003
PshAI GACNNNNGTC 1 cut(s) 1222
Psp124BI GAGCTC 2 cut(s) 643, 1443
Psp6I CCWGG 4 cut(s) 51, 1185, 1219, 1628
PspEI GGTNACC 1 cut(s) 1613
PspFI CCCAGC 2 cut(s) 249, 1406
PspGI CCWGG 4 cut(s) 51, 1185, 1219, 1628
PspN4I GGNNCC 7 cut(s) 63, 247, 342, 853, 1606, 1627, 1684
PspPI GGNCC 3 cut(s) 61, 182, 1189
PstI CTGCAG 1 cut(s) 1240
PsuI RGATCY 2 cut(s) 355, 1162
RsaI GTAC 4 cut(s) 376, 853, 863, 1809
RsaNI GTAC 4 cut(s) 375, 852, 862, 1808
RseI CAYNNNNRTG 1 cut(s) 1008
SacI GAGCTC 2 cut(s) 643, 1443
SalI GTCGAC 1 cut(s) 584
SaqAI TTAA 5 cut(s) 1044, 1385, 1587, 1715, 1840
SatI GCNGC 8 cut(s) 102, 297, 415, 621, 1395, 1398, 1578, 1593
Sau3AI GATC 7 cut(s) 112, 355, 811, 1162, 1182, 1264, 1832
Sau96I GGNCC 3 cut(s) 61, 182, 1189
SchI GAGTC 5 cut(s) 135, 262, 486, 581, 1357
ScrFI CCNGG 5 cut(s) 53, 1187, 1221, 1630, 1690
SduI GDGCHC 4 cut(s) 343, 643, 1351, 1443
SfaNI GCATC 7 cut(s) 52, 76, 113, 559, 693, 851, 1711
SfcI CTRYAG 1 cut(s) 1236
SfoI GGCGCC 1 cut(s) 1606
SinI GGWCC 2 cut(s) 182, 1189
SmiMI CAYNNNNRTG 1 cut(s) 1008
SmlI CTYRAG 2 cut(s) 234, 629
SmoI CTYRAG 2 cut(s) 234, 629
SphI GCATGC 1 cut(s) 830
SseBI AGGCCT 1 cut(s) 627
SsiI CCGC 5 cut(s) 137, 620, 1580, 1671, 1791
SspDI GGCGCC 1 cut(s) 1604
SspI AATATT 1 cut(s) 616
SspMI CTAG 8 cut(s) 152, 164, 522, 681, 756, 1166, 1548, 1560
SstI GAGCTC 2 cut(s) 643, 1443
StuI AGGCCT 1 cut(s) 627
StyD4I CCNGG 5 cut(s) 51, 1185, 1219, 1628, 1688
StyI CCWWGG 4 cut(s) 1192, 1596, 1608, 1794
TaaI ACNGT 4 cut(s) 716, 952, 1619, 1736
TaiI ACGT 2 cut(s) 408, 1146
TaqI TCGA 6 cut(s) 46, 497, 585, 1214, 1293, 1831
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 3 cut(s) 302, 869, 1828
Tru1I TTAA 5 cut(s) 1044, 1385, 1587, 1715, 1840
Tru9I TTAA 5 cut(s) 1044, 1385, 1587, 1715, 1840
TscAI CASTG 2 cut(s) 928, 1254
TseFI GTSAC 5 cut(s) 12, 256, 1613, 1694, 1736
TseI GCWGC 7 cut(s) 101, 296, 414, 1394, 1397, 1577, 1592
Tsp45I GTSAC 5 cut(s) 12, 256, 1613, 1694, 1736
TspDTI ATGAA 4 cut(s) 89, 222, 1416, 1518
TspGWI ACGGA 1 cut(s) 862
TspRI CASTG 2 cut(s) 928, 1254
VneI GTGCAC 1 cut(s) 1347
VpaK11BI GGWCC 2 cut(s) 182, 1189
XapI RAATTY 2 cut(s) 458, 774
XceI RCATGY 3 cut(s) 766, 830, 1007
XcmI CCANNNNNNNNNTGG 1 cut(s) 1801
XmiI GTMKAC 1 cut(s) 585
XmnI GAANNNNTTC 1 cut(s) 1519
XspI CTAG 8 cut(s) 152, 164, 522, 681, 756, 1166, 1548, 1560
Zsp2I ATGCAT 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.