Rh5AG139600

pre-mRNA 3'-splice site binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000360
Physical Location & Seq
Forward (+)
14506289 .. 14510281
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG139600.1

Sequence Viewer

Length: 2289 bp
ATGTTTTCGTCCATGGATACTGCATCTGTTCAAGGTTCTGTTGCTGGTGGGCTTGGGGATCCTGATCAAATTTTTTTTGGTGGATTCCCTTACTACTTTAAAGAAGAACAGATCAGGGAGTTGGTAGAGTCCTTCGGACCCCTTCGCAGTTTTGAACTAGTGACAGATGGAGGCAGAGGAAACAACTCAGTTGCTGCCCATTGCGTTTACCAGGATGTTTCAGTTACAGACATTGCTTGTCATGGCCTGAATGGGATTCGAATCGGTGCCCAGACACTGATTGCTAGGCGTGCTAATCATGGTGCCAACCAGCAGCCGGAACCACATTCTTCTTCTCAAGAAAATTGCAGTATTTGCTGGTGTAGCCTGACAACTGGCCAAGGCCAAGCGATCTTTACTGCTGAGTGCTCCCACCGTTTCCACTACCCTTGTATTGCCAACAATGTCCAGCATGGAAATCTTTACTGCCCTGTCTGCAGAGTAAAATGGGACAAGAATAATGTACCTTTCCAAGTTCCTCCTCCTCAACAGACCAGGATCCCTTTCAGGCAGTTCCCAATGCTGCAGCAACCCCATTGGAATTATCCTCACCTCCCATATCAGCAGCCGCATTGGAATCATCCTCAGTACCCATTTGAGCAGCAACCCCAGGACCCTCCTGAGCCACTTACCTTCTCTGATGATGAACCTCTCCAGTCATTGACCTCACCTGTTCAATCCTCCTCCCACCAGAATGTGACAGTTACGACTTATGCAGAGTACTCTGCTATCTCTGCTGCGGAGTCTAGCTCAAAGTTTCCTGTCCTTGTCAGCATCCGTGCACCACCCCTTAAGGATTCTGAGGGCCATGGTCGTACTCCCATTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGACTGGCTTGAAGCTTAACCTTGTCAAGCGTGCTGTCAAATTTGTCATAGAAAACTTGGGGTCTTCAGACAGACTTTCTGTTGTCTCATTCTCATCAAATTCTAAGAGAGTTCTTCCTCTCCAAAGGATGACTGCTGATGGCCGTGAAAGTGCCATACTAGCCGTCGAGTCTCTTAGAGCAGGTGGTGGAACTAACATTGCCGAAGCACTCAAGAAAGGAACTAAGGTCCTTGAAGATCGAAGGGAAAGGAACCCAGTTGCTAGCATCATCCTCTTATCCGATGGCCAAGATAACTACTGTCGAAGTGGAAGCCAAATTTTGAACCAATTGCCGGCTTCCATTCGTTCTAGTGACATGCAACAGGAAATCCCAGTCCATGCATTTGGGTTTGGCCAAGACCATGATGCAAGCATTATGCATGCTATTTCTGATGCATCCGGTGGTACCTTTTCATTCATTGAATCTGTTGGCATGATACAAGATGGTTTTGCTTTGTGCATTGGTGGTCTTCTCAGTGTAGTGGCTCAGGAGCTTCGCCTCACAGTTAGGTCAGCATCAGCTGGGGTGAAGATTGTTTCAATACCATCAGGGAGACATGTGAGCCAGATTTCTGATGAGGGCCAGCAAGGTGTAGTTGATATTGGAAATATGTATGCAGAAGAGGAGAAACAATTTCTGGTCTACCTGTTAGTTCCACCCTCAGCTTCACATGTTAAGATGCCATTGTTAGATGTAACATGCGTGTATAAAGATCTTGCTTCAAATGAGTTGATGCAGGTGCAAGGTGAGAGAGTAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCAGAAAAGGCAGTGTCTTTGGAGGTTGATCGACAGCGGAACAGAATTGTGGTGGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGATGGGAAACCTGGAAGGTGCACGGGCACTTTTGGCTGAACGAAGAGCAACTGTCTTAGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCTCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGCTAGGGGTGACACAATGACCTCATGTCTCTTTGGTGCCTCCAACCATGTGAATTTCTCCAGCGGTGGCAATGCCTTAATGCAGCAGTCGGGTGCCTCTGCTTGTTTTGGCCTCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCCGGTGCCGCTCCAGGTGGAAGTCAATGCTCTTCTGCAGTTGGTGCTTTTGCTACACCTGCTATGGTTAGAATGGTAGAAAAATCACAGAAGAGCAATCAATCTGGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HA2 Helicase_C OB_NTP_bind RRM_1 S1

Protein Analysis

762

Amino Acids

82.1

Weight (kDa)

5.88

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 24 - 92 2.3e-06 RNA recognition motif
zf-RING_11 PF17123 115 - 145 2.5e-09 RING-like zinc finger
zf-RING_2 PF13639 115 - 160 7.6e-07 Ring finger domain
VWA_CoxE PF05762 285 - 402 2.2e-07 VWA domain containing CoxE-like protein
VWA PF00092 289 - 453 8.3e-24 von Willebrand factor type A domain
VWA_3 PF13768 289 - 452 5.6e-19 von Willebrand factor type A domain
VWA_2 PF13519 290 - 396 2.9e-18 von Willebrand factor type A domain
WAV3_C PF25243 584 - 650 9.1e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 640 - 742 3.6e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1070, 1666, 2242
Acc36I ACCTGC 4 cut(s) 1070, 1666, 2141, 2242
Acc65I GGTACC 1 cut(s) 1343
AccB1I GGYRCC 6 cut(s) 266, 302, 1343, 2066, 2123, 2180
AccBSI CCGCTC 1 cut(s) 2186
AccI GTMKAC 1 cut(s) 1581
AciI CCGC 5 cut(s) 608, 779, 1762, 2094, 2184
AclWI GGATC 4 cut(s) 53, 66, 532, 545
AcoI YGGCCR 4 cut(s) 376, 1039, 1183, 1291
AcsI RAATTY 5 cut(s) 69, 938, 997, 1215, 2083
AcuI CTGAAG 2 cut(s) 948, 2013
AfaI GTAC 6 cut(s) 504, 629, 761, 856, 1345, 1967
AfiI CCNNNNNNNGG 2 cut(s) 316, 1231
AflII CTTAAG 1 cut(s) 830
AflIII ACRYGT 2 cut(s) 1495, 1609
AhdI GACNNNNNGTC 1 cut(s) 2055
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 5 cut(s) 210, 533, 648, 1824, 2188
AluBI AGCT 9 cut(s) 789, 913, 1432, 1460, 1604, 1886, 1892, 1927, 2033
AluI AGCT 9 cut(s) 789, 913, 1432, 1460, 1604, 1886, 1892, 1927, 2033
Alw21I GWGCWC 3 cut(s) 410, 823, 1837
Alw26I GTCTC 6 cut(s) 988, 1074, 1486, 1699, 1907, 2063
Alw44I GTGCAC 2 cut(s) 819, 1833
AlwI GGATC 4 cut(s) 53, 66, 532, 545
AlwNI CAGNNNCTG 2 cut(s) 194, 277
AoxI GGCC 9 cut(s) 244, 376, 382, 844, 1039, 1183, 1291, 1519, 2140
ApaLI GTGCAC 2 cut(s) 819, 1833
ApoI RAATTY 5 cut(s) 69, 938, 997, 1215, 2083
ArsI GACNNNNNNTTYG 2 cut(s) 1698, 1730
Asp718I GGTACC 1 cut(s) 1343
AspS9I GGNCC 5 cut(s) 137, 652, 844, 1126, 1519
AsuHPI GGTGA 5 cut(s) 581, 699, 1477, 1697, 2051
AsuII TTCGAA 1 cut(s) 259
AsuNHI GCTAGC 1 cut(s) 1160
AvaII GGWCC 3 cut(s) 137, 652, 1126
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 4 cut(s) 271, 823, 1837, 1843
BalI TGGCCA 3 cut(s) 378, 1185, 1293
BamHI GGATCC 2 cut(s) 58, 537
BanI GGYRCC 6 cut(s) 266, 302, 1343, 2066, 2123, 2180
BbsI GAAGAC 2 cut(s) 954, 1400
Bbv12I GWGCWC 3 cut(s) 410, 823, 1837
BbvCI CCTCAGC 1 cut(s) 1600
BccI CCATC 7 cut(s) 161, 1031, 1175, 1376, 1492, 1810, 1892
BceAI ACGGC 2 cut(s) 1026, 1046
BciT130I CCWGG 5 cut(s) 212, 535, 650, 1826, 2190
BciVI GTATCC 1 cut(s) 10
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 6 cut(s) 988, 1074, 1486, 1699, 1907, 2063
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 8 cut(s) 158, 285, 786, 1058, 1161, 1248, 2034, 2166
BfmI CTRYAG 5 cut(s) 475, 563, 1725, 2148, 2211
BfrI CTTAAG 1 cut(s) 830
BfuAI ACCTGC 4 cut(s) 1070, 1666, 2141, 2242
BfuI GTATCC 1 cut(s) 10
BglII AGATCT 1 cut(s) 1651
BmcAI AGTACT 1 cut(s) 761
Bme1390I CCNGG 5 cut(s) 212, 535, 650, 1826, 2190
Bme18I GGWCC 3 cut(s) 137, 652, 1126
BmeRI GACNNNNNGTC 1 cut(s) 2055
BmgT120I GGNCC 5 cut(s) 137, 652, 844, 1126, 1519
BmrFI CCNGG 5 cut(s) 212, 535, 650, 1826, 2190
BmrI ACTGGG 2 cut(s) 1148, 1265
BmtI GCTAGC 1 cut(s) 1164
BmuI ACTGGG 2 cut(s) 1148, 1265
BpiI GAAGAC 2 cut(s) 954, 1400
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpmI CTGGAG 3 cut(s) 677, 2074, 2172
Bpu10I CCTNAGC 3 cut(s) 660, 1425, 1600
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 3 cut(s) 321, 1094, 1872
BsaBI GATNNNNATC 2 cut(s) 63, 260
BsaI GGTCTC 2 cut(s) 1699, 1907
BsaJI CCNNGG 4 cut(s) 12, 379, 648, 847
BsaWI WCCGGW 2 cut(s) 1337, 2177
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 1231
Bse118I RCCGGY 1 cut(s) 1231
Bse1I ACTGG 5 cut(s) 379, 694, 907, 1154, 1271
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 4 cut(s) 199, 231, 1095, 2107
Bse8I GATNNNNATC 2 cut(s) 63, 260
BseBI CCWGG 5 cut(s) 212, 535, 650, 1826, 2190
BseDI CCNNGG 4 cut(s) 12, 379, 648, 847
BseJI GATNNNNATC 2 cut(s) 63, 260
BseLI CCNNNNNNNGG 2 cut(s) 316, 1231
BseMI GCAATG 4 cut(s) 199, 231, 1095, 2107
BseNI ACTGG 5 cut(s) 379, 694, 907, 1154, 1271
BseRI GAGGAG 4 cut(s) 510, 513, 712, 1577
BseSI GKGCMC 4 cut(s) 271, 823, 1837, 1843
BseYI CCCAGC 2 cut(s) 1460, 1892
BshFI GGCC 9 cut(s) 246, 378, 384, 846, 1041, 1185, 1293, 1521, 2142
BshNI GGYRCC 6 cut(s) 266, 302, 1343, 2066, 2123, 2180
BsiHKAI GWGCWC 3 cut(s) 410, 823, 1837
BsiSI CCGG 4 cut(s) 317, 1232, 1338, 2178
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 2 cut(s) 316, 1231
BsmAI GTCTC 6 cut(s) 988, 1074, 1486, 1699, 1907, 2063
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 9 cut(s) 246, 378, 384, 846, 1041, 1185, 1293, 1521, 2142
Bso31I GGTCTC 2 cut(s) 1699, 1907
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 5 cut(s) 271, 410, 823, 1837, 1843
Bsp143I GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
Bsp19I CCATGG 2 cut(s) 12, 847
BspACI CCGC 5 cut(s) 608, 779, 1762, 2094, 2184
BspANI GGCC 9 cut(s) 246, 378, 384, 846, 1041, 1185, 1293, 1521, 2142
BspMAI CTGCAG 5 cut(s) 479, 567, 1729, 2152, 2215
BspMI ACCTGC 4 cut(s) 1070, 1666, 2141, 2242
BspOI GCTAGC 1 cut(s) 1164
BspPI GGATC 4 cut(s) 53, 66, 532, 545
BspQI GCTCTTC 3 cut(s) 1852, 2212, 2261
BspT104I TTCGAA 1 cut(s) 259
BspT107I GGYRCC 6 cut(s) 266, 302, 1343, 2066, 2123, 2180
BspTI CTTAAG 1 cut(s) 830
BspTNI GGTCTC 2 cut(s) 1699, 1907
BsrBI CCGCTC 1 cut(s) 2186
BsrDI GCAATG 4 cut(s) 199, 231, 1095, 2107
BsrFI RCCGGY 1 cut(s) 1231
BsrI ACTGG 5 cut(s) 379, 694, 907, 1154, 1271
BssAI RCCGGY 1 cut(s) 1231
BssECI CCNNGG 4 cut(s) 12, 379, 648, 847
BssMI GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
BssT1I CCWWGG 3 cut(s) 12, 379, 847
Bst2UI CCWGG 5 cut(s) 212, 535, 650, 1826, 2190
Bst4CI ACNGT 6 cut(s) 416, 742, 877, 1199, 1444, 1867
Bst6I CTCTTC 4 cut(s) 1554, 1852, 2212, 2261
BstAFI CTTAAG 1 cut(s) 830
BstAPI GCANNNNNTGC 2 cut(s) 354, 2219
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 8 cut(s) 291, 930, 1162, 1233, 1309, 1320, 1523, 1983
BstDSI CCRYGG 2 cut(s) 12, 847
BstKTI GATC 8 cut(s) 61, 67, 114, 393, 540, 1138, 1654, 1756
BstMAI GTCTC 6 cut(s) 988, 1074, 1486, 1699, 1907, 2063
BstMBI GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
BstNI CCWGG 5 cut(s) 212, 535, 650, 1826, 2190
BstNSI RCATGY 5 cut(s) 1258, 1322, 1499, 1613, 1641
BstSCI CCNGG 5 cut(s) 210, 533, 648, 1824, 2188
BstSFI CTRYAG 5 cut(s) 475, 563, 1725, 2148, 2211
BstSLI GKGCMC 4 cut(s) 271, 823, 1837, 1843
BstV2I GAAGAC 2 cut(s) 954, 1400
BstX2I RGATCY 3 cut(s) 58, 537, 1651
BstXI CCANNNNNNTGG 1 cut(s) 1283
BstYI RGATCY 3 cut(s) 58, 537, 1651
Bsu36I CCTNAGG 1 cut(s) 1710
BsuI GTATCC 1 cut(s) 10
BsuRI GGCC 9 cut(s) 246, 378, 384, 846, 1041, 1185, 1293, 1521, 2142
BtgI CCRYGG 2 cut(s) 12, 847
BtsI GCAGTG 1 cut(s) 1743
BtsIMutI CAGTG 3 cut(s) 275, 1420, 1743
BveI ACCTGC 4 cut(s) 1070, 1666, 2141, 2242
Cac8I GCNNGC 8 cut(s) 291, 930, 1162, 1233, 1309, 1320, 1523, 1983
CaiI CAGNNNCTG 2 cut(s) 194, 277
Cfr10I RCCGGY 1 cut(s) 1231
Cfr13I GGNCC 5 cut(s) 137, 652, 844, 1126, 1519
Csp6I GTAC 6 cut(s) 503, 628, 760, 855, 1344, 1966
CspCI CAANNNNNGTGG 2 cut(s) 410, 445
CviQI GTAC 6 cut(s) 503, 628, 760, 855, 1344, 1966
DpnI GATC 8 cut(s) 60, 66, 113, 392, 539, 1137, 1653, 1755
DpnII GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
DraI TTTAAA 1 cut(s) 100
DriI GACNNNNNGTC 1 cut(s) 2055
EaeI YGGCCR 4 cut(s) 376, 1039, 1183, 1291
Eam1104I CTCTTC 4 cut(s) 1554, 1852, 2212, 2261
Eam1105I GACNNNNNGTC 1 cut(s) 2055
EarI CTCTTC 4 cut(s) 1554, 1852, 2212, 2261
Eco130I CCWWGG 3 cut(s) 12, 379, 847
Eco31I GGTCTC 2 cut(s) 1699, 1907
Eco47I GGWCC 3 cut(s) 137, 652, 1126
Eco57I CTGAAG 2 cut(s) 948, 2013
Eco81I CCTNAGG 1 cut(s) 1710
EcoO109I RGGNCCY 2 cut(s) 652, 1126
EcoRII CCWGG 5 cut(s) 210, 533, 648, 1824, 2188
EcoT14I CCWWGG 3 cut(s) 12, 379, 847
EcoT22I ATGCAT 3 cut(s) 1282, 1320, 1336
ErhI CCWWGG 3 cut(s) 12, 379, 847
FaqI GGGAC 1 cut(s) 503
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 1 cut(s) 1581
FspBI CTAG 8 cut(s) 158, 285, 786, 1058, 1161, 1248, 2034, 2166
GsaI CCCAGC 2 cut(s) 1464, 1896
GsuI CTGGAG 3 cut(s) 677, 2074, 2172
HaeIII GGCC 9 cut(s) 246, 378, 384, 846, 1041, 1185, 1293, 1521, 2142
HapII CCGG 4 cut(s) 317, 1232, 1338, 2178
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 9 cut(s) 84, 128, 256, 261, 616, 782, 836, 1067, 1361
HpaII CCGG 4 cut(s) 317, 1232, 1338, 2178
HphI GGTGA 5 cut(s) 581, 699, 1477, 1697, 2051
Hpy166II GTNNAC 4 cut(s) 208, 821, 1582, 1835
Hpy188I TCNGA 7 cut(s) 137, 679, 841, 967, 1180, 1330, 1513
Hpy188III TCNNGA 7 cut(s) 62, 338, 659, 881, 1111, 1427, 2016
Hpy8I GTNNAC 4 cut(s) 208, 821, 1582, 1835
Hpy99I CGWCG 1 cut(s) 1067
HpyAV CCTTC 5 cut(s) 142, 152, 682, 1134, 1823
HpyCH4III ACNGT 6 cut(s) 416, 742, 877, 1199, 1444, 1867
KpnI GGTACC 1 cut(s) 1347
KroI GCCGGC 1 cut(s) 1231
KroNI GCCGGC 1 cut(s) 1233
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
LguI GCTCTTC 3 cut(s) 1852, 2212, 2261
LmnI GCTCC 3 cut(s) 413, 1429, 2191
MaeI CTAG 8 cut(s) 158, 285, 786, 1058, 1161, 1248, 2034, 2166
MaeIII GTNAC 8 cut(s) 160, 223, 736, 742, 871, 1250, 1633, 2039
MalI GATC 8 cut(s) 60, 66, 113, 392, 539, 1137, 1653, 1755
MbiI CCGCTC 1 cut(s) 2186
MboI GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
MfeI CAATTG 1 cut(s) 1226
MflI RGATCY 3 cut(s) 58, 537, 1651
MhlI GDGCHC 5 cut(s) 271, 410, 823, 1837, 1843
MlsI TGGCCA 3 cut(s) 378, 1185, 1293
MluNI TGGCCA 3 cut(s) 378, 1185, 1293
MlyI GAGTC 3 cut(s) 137, 791, 1076
MmeI TCCRAC 1 cut(s) 2097
Mox20I TGGCCA 3 cut(s) 378, 1185, 1293
Mph1103I ATGCAT 3 cut(s) 1282, 1320, 1336
MroNI GCCGGC 1 cut(s) 1231
MscI TGGCCA 3 cut(s) 378, 1185, 1293
MseI TTAA 5 cut(s) 99, 831, 915, 1614, 2108
MslI CAYNNNNRTG 1 cut(s) 732
Msp20I TGGCCA 3 cut(s) 378, 1185, 1293
MspA1I CMGCKG 3 cut(s) 1460, 1762, 2094
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 4 cut(s) 317, 1232, 1338, 2178
MspR9I CCNGG 5 cut(s) 212, 535, 650, 1826, 2190
MunI CAATTG 1 cut(s) 1226
MvaI CCWGG 5 cut(s) 212, 535, 650, 1826, 2190
NaeI GCCGGC 1 cut(s) 1233
NcoI CCATGG 2 cut(s) 12, 847
NdeII GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
NgoMIV GCCGGC 1 cut(s) 1231
NheI GCTAGC 1 cut(s) 1160
NmuCI GTSAC 4 cut(s) 160, 736, 1250, 2039
NsiI ATGCAT 3 cut(s) 1282, 1320, 1336
NspI RCATGY 5 cut(s) 1258, 1322, 1499, 1613, 1641
NspV TTCGAA 1 cut(s) 259
PaeI GCATGC 1 cut(s) 1322
PaqCI CACCTGC 3 cut(s) 1070, 1666, 2242
PciI ACATGT 2 cut(s) 1495, 1609
PciSI GCTCTTC 3 cut(s) 1852, 2212, 2261
PdiI GCCGGC 1 cut(s) 1233
PfeI GAWTC 6 cut(s) 84, 256, 261, 616, 836, 1361
PleI GAGTC 3 cut(s) 136, 790, 1075
PpsI GAGTC 3 cut(s) 136, 790, 1075
PpuMI RGGWCCY 2 cut(s) 652, 1126
PscI ACATGT 2 cut(s) 1495, 1609
Psp5II RGGWCCY 2 cut(s) 652, 1126
Psp6I CCWGG 5 cut(s) 210, 533, 648, 1824, 2188
PspFI CCCAGC 2 cut(s) 1460, 1892
PspGI CCWGG 5 cut(s) 210, 533, 648, 1824, 2188
PspPI GGNCC 5 cut(s) 137, 652, 844, 1126, 1519
PspPPI RGGWCCY 2 cut(s) 652, 1126
PstI CTGCAG 5 cut(s) 479, 567, 1729, 2152, 2215
PstNI CAGNNNCTG 2 cut(s) 194, 277
PsuI RGATCY 3 cut(s) 58, 537, 1651
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 6 cut(s) 504, 629, 761, 856, 1345, 1967
RsaNI GTAC 6 cut(s) 503, 628, 760, 855, 1344, 1966
RseI CAYNNNNRTG 1 cut(s) 732
SapI GCTCTTC 3 cut(s) 1852, 2212, 2261
SaqAI TTAA 5 cut(s) 99, 831, 915, 1614, 2108
Sau3AI GATC 8 cut(s) 58, 64, 111, 390, 537, 1135, 1651, 1753
Sau96I GGNCC 5 cut(s) 137, 652, 844, 1126, 1519
SbfI CCTGCAGG 1 cut(s) 2152
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 3 cut(s) 137, 791, 1076
ScrFI CCNGG 5 cut(s) 212, 535, 650, 1826, 2190
SdaI CCTGCAGG 1 cut(s) 2152
SduI GDGCHC 5 cut(s) 271, 410, 823, 1837, 1843
SfcI CTRYAG 5 cut(s) 475, 563, 1725, 2148, 2211
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 3 cut(s) 137, 652, 1126
SmiMI CAYNNNNRTG 1 cut(s) 732
SmlI CTYRAG 4 cut(s) 336, 830, 1109, 1887
SmoI CTYRAG 4 cut(s) 336, 830, 1109, 1887
SpeI ACTAGT 1 cut(s) 157
SphI GCATGC 1 cut(s) 1322
Sse8387I CCTGCAGG 1 cut(s) 2152
SsiI CCGC 5 cut(s) 608, 779, 1762, 2094, 2184
SspMI CTAG 8 cut(s) 158, 285, 786, 1058, 1161, 1248, 2034, 2166
StyD4I CCNGG 5 cut(s) 210, 533, 648, 1824, 2188
StyI CCWWGG 3 cut(s) 12, 379, 847
TaaI ACNGT 6 cut(s) 416, 742, 877, 1199, 1444, 1867
TaqI TCGA 6 cut(s) 259, 1065, 1138, 1201, 1756, 2017
TatI WGTACW 1 cut(s) 759
TauI GCSGC 2 cut(s) 610, 2186
TfiI GAWTC 6 cut(s) 84, 256, 261, 616, 836, 1361
Tru1I TTAA 5 cut(s) 99, 831, 915, 1614, 2108
Tru9I TTAA 5 cut(s) 99, 831, 915, 1614, 2108
TscAI CASTG 3 cut(s) 282, 1420, 1743
TseFI GTSAC 4 cut(s) 160, 736, 1250, 2039
Tsp45I GTSAC 4 cut(s) 160, 736, 1250, 2039
TspDTI ATGAA 4 cut(s) 699, 1341, 1345, 2163
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 3 cut(s) 282, 1420, 1743
Vha464I CTTAAG 1 cut(s) 830
VneI GTGCAC 2 cut(s) 819, 1833
VpaK11BI GGWCC 3 cut(s) 137, 652, 1126
XapI RAATTY 5 cut(s) 69, 938, 997, 1215, 2083
XceI RCATGY 5 cut(s) 1258, 1322, 1499, 1613, 1641
XmiI GTMKAC 1 cut(s) 1581
XspI CTAG 8 cut(s) 158, 285, 786, 1058, 1161, 1248, 2034, 2166
ZrmI AGTACT 1 cut(s) 761
Zsp2I ATGCAT 3 cut(s) 1282, 1320, 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.