RchiOBHm_Chr5g0019121

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000360
Physical Location & Seq
Forward (+)
13586144 .. 13588090
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29926

Sequence Viewer

Length: 1947 bp
ATGATTGCTAATATTGTATTAAATTGTTGCTTTCGTGTACAGAAAAATTGCGCAATCTGCTGGAGTAACGTGACAACAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTCCTTCCACTACCCTTGCATTGCCAACAATGTCCAGCATGGAAATCTTTGCTGCCCCATCTGCCGAGCAAAATGGGATAAGAATAATGTTCCTTTCCAAGTTCCCCCGCTTCAACAAAACAACTTGGGTGCACATGGCTTCGCTGATGATGAACCTCTCCCGTTCACCTCACCGGCTCAATCTTCTGTCCCCCAGAATGTCACAATCAAAACTCATACAGAGACCTCTGCTATCCCTGCTGCCGATTCACGCCCACAATATCCTGTTCTAGTCAGCATCTGTGCACCACCCCTTCAGGATCCTGATGGCGAAGTCCGTACACCGATTGACCTTGTAACAGTTCTAGACGTAAGTGGCAGCATGCAGGGCCAAAAGCTTCACCTTGTCAAGCAAGCTATCAAATTTGTCATAGAAAACATGGGGTCTTCAGATAGACTTTCTATAGTTTCATTCTCAACAAATTCTAGAAGAGTCCTTCCTCTTCGAAGAATGACCGTTGATGGCCGTGAAAGTGCAATTGAAGCTGTCGAATCTCTTGTAGCAGGTGGTGGAACTGACATTGCAGAAGGACTCAAAAAAGGAACACAAGTCCTTGAAGATCGGAGGCAAAGGAACCCAGTTGCTAGCATCATTCTCTTATCCGATGGCCAAGATAACTACTGTCGAAGCCCAAGCCAAATGTTGAAGAAATTGCCTGCTTCAATTCGTTCCAGCGACATGCAACATGAAATCCCTGTCCACACATTTGGGTTCGGCAACGACCATGATGCAAAGATTATGCATGCTATATCTGAGGAATCCCGGGGCACCTTTTCATACATTGAATCAGTTGGCATGATACAAGACGCCTTTGCTCTGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAACTTCGCCTCACAGTGAGGTCGGCATCACATGGAGCGAAGATTGTATCAATACCATCAGGGAGATATGTGAGTCAGATTTCTGATGACGGCTCGCAAGGTTTAGTTGATATTGGAAATATGTACGCAGAAGAGGTGAAACAATTTCTGGTGTATCTCTTAGTTCCACAATCCTCAGCTTCAGAGACTAAGACATCATTGGTGGACGTCTTATGCGTGTACAGAGATCTAGCTTCAAATGAGTTTATCCAGCTCCAAAGTGGGAGAGTAGAGATATCAAGACCACAGTTTTGTTCTCCTACAGAATTGGCAGTGTGTTTGGAGGTTGATCGCCAGCGGAACAGACTTTTGGTGGCTGATGCTATTGGAGAGGCTCAACGCTTGGCTGAAATGGGAAATTTGGAGGGTGCACGCGAAATTTTGACTCAACGAAGAGAAACTCTCTTAGCATCAGCAGCAGCCAAAGCTGGGGATAGTCTCAGTAATATGTTTGAAACTGAGCTTAAAGAAATCAGGGATAAAATGGCAAGTATGAAGTTGTACGAAGAGGCGGGGCGTGCTTATGCTCTGGCTGGAATGAGTTCACATTCACGCCAGAGGGCCTCTGCTAGGGGTAACGCAGAAGGCTCGTGCCTGGCCATGAAGGGTCGCAAACAACTTGCTACAAAAGCAGCGGGTCGTGCTTCCGGGGGTCGCGGTGTAGCAGTGACTCTTGCTGCCGGGGGTTTTGGTGGCAAGGGTGGTCCCAAAGGCAAACGCTCTGCAATGGATCACTTAGTGGCTGCTATGGGAGACTCTGATGAAGATGATGATATGGGCTTTGCTTTATGTGACGGAGGTTCATTTGACGGAGGCAATGATGTCCATCGAGGTGCTTTTGAAACACCAGCAATGGTAAGAATGGTACAGAAATCACAGCAGAAGAATCAATCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

648

Amino Acids

69.81

Weight (kDa)

6.55

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_11 PF17123 16 - 46 6.1e-10 RING-like zinc finger
zf-RING_2 PF13639 16 - 61 2e-06 Ring finger domain
VWA_CoxE PF05762 145 - 263 1.7e-08 VWA domain containing CoxE-like protein
VWA PF00092 149 - 317 1.5e-25 von Willebrand factor type A domain
VWA_3 PF13768 149 - 310 4.3e-20 von Willebrand factor type A domain
VWA_2 PF13519 150 - 256 3e-19 von Willebrand factor type A domain
WAV3_C PF25243 445 - 511 1e-05 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 500 - 542 9.8e-10 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 650
AatII GACGTC 1 cut(s) 1218
Acc16I TGCGCA 1 cut(s) 52
Acc36I ACCTGC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 923
AccII CGCG 2 cut(s) 1422, 1704
AciI CCGC 5 cut(s) 224, 1345, 1559, 1682, 1704
AclWI GGATC 3 cut(s) 410, 423, 1785
AcoI YGGCCR 3 cut(s) 619, 763, 1644
AcsI RAATTY 4 cut(s) 518, 577, 1405, 1425
AcuI CTGAAG 3 cut(s) 395, 528, 1173
AcyI GRCGYC 2 cut(s) 963, 1215
AdeI CACNNNGTG 1 cut(s) 1786
AfaI GTAC 6 cut(s) 39, 436, 1133, 1229, 1550, 1914
AfiI CCNNNNNNNGG 2 cut(s) 1476, 1617
AgsI TTSAA 9 cut(s) 230, 638, 713, 802, 819, 941, 1245, 1502, 1889
AjnI CCWGG 1 cut(s) 1641
AjuI GAANNNNNNNTTGG 2 cut(s) 1340, 1372
AluBI AGCT 8 cut(s) 493, 512, 641, 1187, 1241, 1261, 1475, 1510
AluI AGCT 8 cut(s) 493, 512, 641, 1187, 1241, 1261, 1475, 1510
Alw21I GWGCWC 4 cut(s) 113, 250, 403, 1420
Alw26I GTCTC 4 cut(s) 332, 1187, 1490, 1794
Alw44I GTGCAC 3 cut(s) 246, 399, 1416
AlwI GGATC 3 cut(s) 410, 423, 1785
AlwNI CAGNNNCTG 1 cut(s) 396
Ama87I CYCGRG 1 cut(s) 918
AoxI GGCC 6 cut(s) 85, 484, 619, 763, 1608, 1644
ApaLI GTGCAC 3 cut(s) 246, 399, 1416
ApeKI GCWGC 8 cut(s) 168, 356, 474, 1463, 1466, 1679, 1724, 1790
ApoI RAATTY 4 cut(s) 518, 577, 1405, 1425
ArsI GACNNNNNNTTYG 2 cut(s) 1281, 1313
Asp700I GAANNNNTTC 1 cut(s) 1588
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 484, 1608, 1751
AsuC2I CCSGG 4 cut(s) 919, 920, 1696, 1729
AsuHPI GGTGA 4 cut(s) 274, 279, 488, 1156
AsuII TTCGAA 1 cut(s) 601
AsuNHI GCTAGC 1 cut(s) 740
AvaI CYCGRG 1 cut(s) 918
AvaII GGWCC 1 cut(s) 1751
BaeGI GKGCMC 4 cut(s) 250, 403, 926, 1420
BalI TGGCCA 2 cut(s) 765, 1646
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 923
BauI CACGAG 1 cut(s) 1636
BbsI GAAGAC 2 cut(s) 534, 980
Bbv12I GWGCWC 4 cut(s) 113, 250, 403, 1420
BbvCI CCTCAGC 1 cut(s) 1183
BbvI GCAGC 8 cut(s) 155, 343, 486, 1475, 1478, 1691, 1711, 1777
BccI CCATC 7 cut(s) 101, 182, 416, 611, 755, 1072, 1881
BceAI ACGGC 2 cut(s) 606, 1114
BcgI CGANNNNNNTGC 4 cut(s) 866, 900, 1617, 1651
BciT130I CCWGG 1 cut(s) 1643
BcnI CCSGG 4 cut(s) 919, 920, 1696, 1729
BcoDI GTCTC 4 cut(s) 332, 1187, 1490, 1794
BfaI CTAG 6 cut(s) 386, 461, 582, 741, 1238, 1617
BfmI CTRYAG 2 cut(s) 558, 1308
BfuAI ACCTGC 1 cut(s) 650
BglII AGATCT 1 cut(s) 1234
BisI GCNGC 8 cut(s) 169, 357, 475, 1464, 1467, 1680, 1725, 1791
BlsI GCNGC 8 cut(s) 170, 358, 476, 1465, 1468, 1681, 1726, 1792
Bme1390I CCNGG 5 cut(s) 919, 920, 1643, 1696, 1729
Bme18I GGWCC 1 cut(s) 1751
BmeT110I CYCGRG 1 cut(s) 918
BmgT120I GGNCC 3 cut(s) 484, 1608, 1751
BmiI GGNNCC 4 cut(s) 417, 731, 925, 1753
BmrFI CCNGG 5 cut(s) 919, 920, 1643, 1696, 1729
BmrI ACTGGG 1 cut(s) 728
BmsI GCATC 6 cut(s) 402, 753, 874, 1043, 1357, 1466
BmtI GCTAGC 1 cut(s) 744
BmuI ACTGGG 1 cut(s) 728
BpiI GAAGAC 2 cut(s) 534, 980
BplI GAGNNNNNCTC 2 cut(s) 1434, 1466
BpmI CTGGAG 1 cut(s) 82
Bpu10I CCTNAGC 2 cut(s) 1005, 1183
Bpu14I TTCGAA 1 cut(s) 601
BpuMI CCSGG 4 cut(s) 919, 920, 1696, 1729
BsaBI GATNNNNATC 1 cut(s) 1872
BsaHI GRCGYC 2 cut(s) 963, 1215
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 918, 919, 1695, 1728
Bsc4I CCNNNNNNNGG 2 cut(s) 1476, 1617
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 734
Bse3DI GCAATG 5 cut(s) 135, 675, 1779, 1870, 1905
Bse8I GATNNNNATC 1 cut(s) 1872
BseBI CCWGG 1 cut(s) 1643
BseDI CCNNGG 4 cut(s) 918, 919, 1695, 1728
BseJI GATNNNNATC 1 cut(s) 1872
BseLI CCNNNNNNNGG 2 cut(s) 1476, 1617
BseMI GCAATG 5 cut(s) 135, 675, 1779, 1870, 1905
BseMII CTCAG 7 cut(s) 96, 900, 1006, 1019, 1197, 1497, 1501
BseNI ACTGG 1 cut(s) 734
BseSI GKGCMC 4 cut(s) 250, 403, 926, 1420
BseXI GCAGC 8 cut(s) 155, 343, 486, 1475, 1478, 1691, 1711, 1777
BseYI CCCAGC 1 cut(s) 1475
Bsh1236I CGCG 2 cut(s) 1422, 1704
BshFI GGCC 6 cut(s) 87, 486, 621, 765, 1610, 1646
BshNI GGYRCC 1 cut(s) 923
BsiHKAI GWGCWC 4 cut(s) 113, 250, 403, 1420
BsiHKCI CYCGRG 1 cut(s) 918
BsiSI CCGG 4 cut(s) 290, 919, 1695, 1728
BslFI GGGAC 2 cut(s) 290, 1737
BslI CCNNNNNNNGG 2 cut(s) 1476, 1617
BsmAI GTCTC 4 cut(s) 332, 1187, 1490, 1794
BsmFI GGGAC 2 cut(s) 290, 1737
BsnI GGCC 6 cut(s) 87, 486, 621, 765, 1610, 1646
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 918
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 5 cut(s) 113, 250, 403, 926, 1420
Bsp1407I TGTACA 2 cut(s) 37, 1227
Bsp143I GATC 5 cut(s) 415, 715, 1234, 1336, 1777
BspACI CCGC 5 cut(s) 224, 1345, 1559, 1682, 1704
BspANI GGCC 6 cut(s) 87, 486, 621, 765, 1610, 1646
BspCNI CTCAG 7 cut(s) 97, 901, 1005, 1018, 1196, 1498, 1500
BspFNI CGCG 2 cut(s) 1422, 1704
BspLI GGNNCC 4 cut(s) 417, 731, 925, 1753
BspMI ACCTGC 1 cut(s) 650
BspOI GCTAGC 1 cut(s) 744
BspPI GGATC 3 cut(s) 410, 423, 1785
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 5 cut(s) 135, 675, 1779, 1870, 1905
BsrFI RCCGGY 1 cut(s) 289
BsrGI TGTACA 2 cut(s) 37, 1227
BsrI ACTGG 1 cut(s) 734
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 4 cut(s) 918, 919, 1695, 1728
BssMI GATC 5 cut(s) 415, 715, 1234, 1336, 1777
BssNI GRCGYC 2 cut(s) 963, 1215
BssSI CACGAG 1 cut(s) 1636
Bst2BI CACGAG 1 cut(s) 1636
Bst2UI CCWGG 1 cut(s) 1643
Bst4CI ACNGT 5 cut(s) 457, 613, 779, 1024, 1296
Bst6I CTCTTC 5 cut(s) 580, 603, 1134, 1435, 1548
BstACI GRCGYC 2 cut(s) 963, 1215
BstAUI TGTACA 2 cut(s) 37, 1227
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 9 cut(s) 479, 510, 742, 813, 900, 1103, 1343, 1420, 1566
BstENI CCTNNNNNAGG 1 cut(s) 1615
BstFNI CGCG 2 cut(s) 1422, 1704
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 5 cut(s) 418, 718, 1237, 1339, 1780
BstMAI GTCTC 4 cut(s) 332, 1187, 1490, 1794
BstMBI GATC 5 cut(s) 415, 715, 1234, 1336, 1777
BstNI CCWGG 1 cut(s) 1643
BstNSI RCATGY 3 cut(s) 481, 838, 902
BstSCI CCNGG 5 cut(s) 917, 918, 1641, 1694, 1727
BstSFI CTRYAG 2 cut(s) 558, 1308
BstSLI GKGCMC 4 cut(s) 250, 403, 926, 1420
BstUI CGCG 2 cut(s) 1422, 1704
BstV1I GCAGC 8 cut(s) 155, 343, 486, 1475, 1478, 1691, 1711, 1777
BstV2I GAAGAC 2 cut(s) 534, 980
BstX2I RGATCY 2 cut(s) 415, 1234
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 2 cut(s) 415, 1234
BsuRI GGCC 6 cut(s) 87, 486, 621, 765, 1610, 1646
BtsI GCAGTG 3 cut(s) 99, 1326, 1719
BtsIMutI CAGTG 5 cut(s) 99, 1000, 1029, 1326, 1719
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 9 cut(s) 479, 510, 742, 813, 900, 1103, 1343, 1420, 1566
CaiI CAGNNNCTG 1 cut(s) 396
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 3 cut(s) 484, 1608, 1751
Cfr9I CCCGGG 1 cut(s) 918
CseI GACGC 1 cut(s) 971
Csp6I GTAC 6 cut(s) 38, 435, 1132, 1228, 1549, 1913
CspCI CAANNNNNGTGG 2 cut(s) 113, 148
CviQI GTAC 6 cut(s) 38, 435, 1132, 1228, 1549, 1913
DpnI GATC 5 cut(s) 417, 717, 1236, 1338, 1779
DpnII GATC 5 cut(s) 415, 715, 1234, 1336, 1777
DraIII CACNNNGTG 1 cut(s) 1786
EaeI YGGCCR 3 cut(s) 619, 763, 1644
Eam1104I CTCTTC 5 cut(s) 580, 603, 1134, 1435, 1548
EarI CTCTTC 5 cut(s) 580, 603, 1134, 1435, 1548
Eco31I GGTCTC 1 cut(s) 332
Eco32I GATATC 1 cut(s) 1284
Eco47I GGWCC 1 cut(s) 1751
Eco57I CTGAAG 3 cut(s) 395, 528, 1173
Eco88I CYCGRG 1 cut(s) 918
EcoNI CCTNNNNNAGG 1 cut(s) 1615
EcoO109I RGGNCCY 1 cut(s) 1608
EcoRII CCWGG 1 cut(s) 1641
EcoRV GATATC 1 cut(s) 1284
EcoT22I ATGCAT 1 cut(s) 900
FaqI GGGAC 2 cut(s) 290, 1737
FauI CCCGC 3 cut(s) 231, 1552, 1675
Fnu4HI GCNGC 8 cut(s) 169, 357, 475, 1464, 1467, 1680, 1725, 1791
Fsp4HI GCNGC 8 cut(s) 169, 357, 475, 1464, 1467, 1680, 1725, 1791
FspBI CTAG 6 cut(s) 386, 461, 582, 741, 1238, 1617
FspI TGCGCA 1 cut(s) 52
GlaI GCGC 1 cut(s) 52
GluI GCNGC 8 cut(s) 169, 357, 475, 1464, 1467, 1680, 1725, 1791
GsaI CCCAGC 1 cut(s) 1479
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 6 cut(s) 87, 486, 621, 765, 1610, 1646
HapII CCGG 4 cut(s) 290, 919, 1695, 1728
HgaI GACGC 1 cut(s) 971
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 2 cut(s) 963, 1215
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 491
HpaII CCGG 4 cut(s) 290, 919, 1695, 1728
HphI GGTGA 4 cut(s) 274, 279, 488, 1156
Hpy188I TCNGA 8 cut(s) 547, 720, 760, 910, 1086, 1093, 1192, 1807
Hpy188III TCNNGA 6 cut(s) 413, 419, 461, 582, 1007, 1287
HpyAV CCTTC 6 cut(s) 130, 419, 602, 677, 1625, 1645
HpyCH4III ACNGT 5 cut(s) 457, 613, 779, 1024, 1296
HpyCH4IV ACGT 3 cut(s) 69, 465, 1215
HpySE526I ACGT 3 cut(s) 69, 465, 1215
Hsp92I GRCGYC 2 cut(s) 963, 1215
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 5 cut(s) 415, 715, 1234, 1336, 1777
LmnI GCTCC 2 cut(s) 1043, 1266
Lsp1109I GCAGC 8 cut(s) 155, 343, 486, 1475, 1478, 1691, 1711, 1777
LweI GCATC 6 cut(s) 402, 753, 874, 1043, 1357, 1466
MaeI CTAG 6 cut(s) 386, 461, 582, 741, 1238, 1617
MaeII ACGT 3 cut(s) 69, 465, 1215
MaeIII GTNAC 7 cut(s) 65, 70, 316, 451, 1622, 1714, 1838
MalI GATC 5 cut(s) 417, 717, 1236, 1338, 1779
MboI GATC 5 cut(s) 415, 715, 1234, 1336, 1777
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 2 cut(s) 415, 1234
MhlI GDGCHC 5 cut(s) 113, 250, 403, 926, 1420
MlsI TGGCCA 2 cut(s) 765, 1646
MluNI TGGCCA 2 cut(s) 765, 1646
MlyI GAGTC 6 cut(s) 597, 681, 1090, 1426, 1711, 1796
Mox20I TGGCCA 2 cut(s) 765, 1646
Mph1103I ATGCAT 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 1588
MscI TGGCCA 2 cut(s) 765, 1646
MseI TTAA 2 cut(s) 20, 1512
MslI CAYNNNNRTG 1 cut(s) 1878
Msp20I TGGCCA 2 cut(s) 765, 1646
MspA1I CMGCKG 2 cut(s) 1345, 1682
MspI CCGG 4 cut(s) 290, 919, 1695, 1728
MspR9I CCNGG 5 cut(s) 919, 920, 1643, 1696, 1729
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 1643
MvnI CGCG 2 cut(s) 1422, 1704
NciI CCSGG 4 cut(s) 919, 920, 1696, 1729
NdeII GATC 5 cut(s) 415, 715, 1234, 1336, 1777
NheI GCTAGC 1 cut(s) 740
NlaIV GGNNCC 4 cut(s) 417, 731, 925, 1753
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 4 cut(s) 70, 316, 1714, 1838
NsbI TGCGCA 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 3 cut(s) 481, 838, 902
NspV TTCGAA 1 cut(s) 601
PaeI GCATGC 2 cut(s) 481, 902
PaqCI CACCTGC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 1588
PfeI GAWTC 5 cut(s) 362, 647, 914, 941, 1933
PkrI GCNGC 8 cut(s) 170, 358, 476, 1465, 1468, 1681, 1726, 1792
PleI GAGTC 6 cut(s) 596, 681, 1089, 1426, 1711, 1796
PpsI GAGTC 6 cut(s) 596, 681, 1089, 1426, 1711, 1796
Psp6I CCWGG 1 cut(s) 1641
PspFI CCCAGC 1 cut(s) 1475
PspGI CCWGG 1 cut(s) 1641
PspN4I GGNNCC 4 cut(s) 417, 731, 925, 1753
PspPI GGNCC 3 cut(s) 484, 1608, 1751
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 415, 1234
RsaI GTAC 6 cut(s) 39, 436, 1133, 1229, 1550, 1914
RsaNI GTAC 6 cut(s) 38, 435, 1132, 1228, 1549, 1913
RseI CAYNNNNRTG 1 cut(s) 1878
SaqAI TTAA 2 cut(s) 20, 1512
SatI GCNGC 8 cut(s) 169, 357, 475, 1464, 1467, 1680, 1725, 1791
Sau3AI GATC 5 cut(s) 415, 715, 1234, 1336, 1777
Sau96I GGNCC 3 cut(s) 484, 1608, 1751
SchI GAGTC 6 cut(s) 597, 681, 1090, 1426, 1711, 1796
ScrFI CCNGG 5 cut(s) 919, 920, 1643, 1696, 1729
SduI GDGCHC 5 cut(s) 113, 250, 403, 926, 1420
SfaNI GCATC 6 cut(s) 402, 753, 874, 1043, 1357, 1466
SfcI CTRYAG 2 cut(s) 558, 1308
SfuI TTCGAA 1 cut(s) 601
SinI GGWCC 1 cut(s) 1751
SmaI CCCGGG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 1878
SphI GCATGC 2 cut(s) 481, 902
SsiI CCGC 5 cut(s) 224, 1345, 1559, 1682, 1704
SspI AATATT 1 cut(s) 13
SspMI CTAG 6 cut(s) 386, 461, 582, 741, 1238, 1617
StyD4I CCNGG 5 cut(s) 917, 918, 1641, 1694, 1727
TaaI ACNGT 5 cut(s) 457, 613, 779, 1024, 1296
TaiI ACGT 3 cut(s) 72, 468, 1218
TaqI TCGA 4 cut(s) 601, 645, 781, 1876
TatI WGTACW 2 cut(s) 37, 1227
TfiI GAWTC 5 cut(s) 362, 647, 914, 941, 1933
Tru1I TTAA 2 cut(s) 20, 1512
Tru9I TTAA 2 cut(s) 20, 1512
TscAI CASTG 5 cut(s) 106, 1000, 1029, 1326, 1719
TseFI GTSAC 4 cut(s) 70, 316, 1714, 1838
TseI GCWGC 8 cut(s) 168, 356, 474, 1463, 1466, 1679, 1724, 1790
Tsp45I GTSAC 4 cut(s) 70, 316, 1714, 1838
TspDTI ATGAA 8 cut(s) 282, 555, 858, 921, 1556, 1664, 1824, 1839
TspGWI ACGGA 3 cut(s) 422, 1857, 1872
TspMI CCCGGG 1 cut(s) 918
TspRI CASTG 5 cut(s) 106, 1000, 1029, 1326, 1719
VneI GTGCAC 3 cut(s) 246, 399, 1416
VpaK11BI GGWCC 1 cut(s) 1751
XagI CCTNNNNNAGG 1 cut(s) 1615
XapI RAATTY 4 cut(s) 518, 577, 1405, 1425
XbaI TCTAGA 2 cut(s) 460, 581
XceI RCATGY 3 cut(s) 481, 838, 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 1265
XmaI CCCGGG 1 cut(s) 918
XmnI GAANNNNTTC 1 cut(s) 1588
XspI CTAG 6 cut(s) 386, 461, 582, 741, 1238, 1617
ZraI GACGTC 1 cut(s) 1216
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.