RLG00000032424

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000360
Physical Location & Seq
Forward (+)
15050694 .. 15053327
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032424

Sequence Viewer

Length: 1899 bp
ATGAATTCGTCTGAGGCTGCATCTGATCAAAACAATTGCGGAATTTGCAGGAGAAGCCTGACAAGAGGGCTAAGCGAAGCCATCTTCACTGCTGAGTGCTCGCACGTTTTCCACTACCATTGCATTCTCAAAAATGTCCAGCAAGGAAATCTTTGCTGCCCTGTCTGCCAAGCAAAGTGGAATAAGGATAATGTACCTTTCCAGGTTCCCCCTCCTCAGAACAACAACACAATCCCTTGTCACTTCACATTCCCTTCACAGCAGCCACCCCATTGGTTCTATCCTCAGTATTCATCCATGCCAGAACCCGAGTACCCTCCTGAGCCACCTACCTTCTCTGACGATGAACCTCTCCCATCGTGCACCTCATCCATTCAATCCTCTGCCCCGCAGAATATAACAGTCAAGACTCATACAGAGTCATCCGCTATCTCTTCTGCAGAATCAATCCCAAATTTTCCTGTCCTTGTCAGCATTCTTGCACCACCCCTTCGGGATTCTGAGGGCCATGGTCGTACTCCCATTGACCTTGTGACAGTTCTGGATGTCAGTGGCAGCATGGAAGGCACAAAGCTTTCCCTACTCAAGCAAGCTGTCACATTTGTCATACAAAACTTGGGGCCTTCGGATAGACTTTCTGTAGTTTCGTTCTCATCAAGTTGTAGAAGAGTCTTTCCTCTCCAGAGAATGTCTGTTGATGGCCGTGAAAGTGCTGTCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCTGAAGGACTCAGGAAAGGAATTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGTATCATCCTCTTATCTGATGGCATAGATACATACAATCGAACTGCAAACCGACTGTTGAACCAGTTGCCAGATTCGATTCGTTCTAGCAACATGCAACATGAAATCCCAGTCCACACATTTGGATTTGGCTACGACCATGATCCCAACATTATGCATGCTATATCTGATCTGTCAGGTGGCACCTTTTCGTTCATTGAATCAGTTGGAATGATACAAGACTCCTTTGCTCTGTGTATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCGCCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACACGTGAATGAGATTTCTGATGAGGGTCAACAAGGTGTTGTTCACGTTGGAAATATGTACGCAGAAGAGGAGAAACAATTTCTGGTCTACCTCTTAGTTCCAGAATCCTCAGGTCCGTATACTAAGATGTCATTGTTAGATGTGTTATGCATGTACAAAGATCTAGCTTCCAATGAACTCATCCAGGTGCAAGGTGAGAAAATAGAGATACTGAGACCCGAGGTTTGTTCCCCTGCTGAAAAGGCAGTTTCTTTGGAGGTTGATCGGCAGCGGAACAGAATTTTGGTGGCTGAAGCTATTGCTGAGGCACAAAGGTTGGCTGAGATGGGAAATATTGAGGGTGCACAGGCTCTATTGGCTCAACGGAAAGAAATTCTATTAACATCTCCAGCATCCCAAGCTGGGGATTATCACAGTAATTTGTTTGAAACTGAGCTTAGAGAAATCATGGAGAGAATGGCATCTATGAAGTTGTATAAACAAACCGGGCGTGCTTACGCTCTTGCAGGAATCAGTTCCCATAAATCACAAAGGGCCAGTGCTAGGGGTGACACAAGAGCCTCCTGTCTCAATTCGGGTTCAGTTGTCTCCAGGAAGAGGGGTCTCCGTAGTCGCAACAGTACCTACAGTTTTGCTCCAGGAGAGTTGGGTCAACCACCTGCCGCAGTTGGTGCTTTCGAAACACCTGCTATGGTCAGAATGGTACGAAAATCACAGAATAGAAATCAACCTGGGCCATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

633

Amino Acids

69.16

Weight (kDa)

6.34

Isoelectric Point (pI)

65.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 11 - 57 7.4e-06 Ring finger domain
zf-RING_11 PF17123 12 - 42 2.6e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 13 - 56 5.3e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 172 - 297 1.4e-08 VWA domain containing CoxE-like protein
VWA PF00092 176 - 341 2.5e-21 von Willebrand factor type A domain
VWA_3 PF13768 176 - 339 1.7e-18 von Willebrand factor type A domain
VWA_2 PF13519 177 - 282 2.2e-18 von Willebrand factor type A domain
WAV3_C PF25243 472 - 539 1.4e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 528 - 624 2.2e-10 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 731, 1822, 1849
Acc36I ACCTGC 3 cut(s) 731, 1822, 1849
AccB1I GGYRCC 1 cut(s) 1004
AccI GTMKAC 2 cut(s) 1242, 1274
AciI CCGC 5 cut(s) 39, 389, 426, 1426, 1818
AclWI GGATC 1 cut(s) 959
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 5 cut(s) 4, 42, 454, 1434, 1527
AcuI CTGAAG 2 cut(s) 783, 1467
AcvI CACGTG 1 cut(s) 1159
AfaI GTAC 7 cut(s) 195, 314, 517, 1214, 1310, 1777, 1860
AfiI CCNNNNNNNGG 4 cut(s) 1557, 1558, 1698, 1752
AflIII ACRYGT 1 cut(s) 1156
AgsI TTSAA 5 cut(s) 377, 794, 883, 1022, 1583
AjnI CCWGG 6 cut(s) 201, 1120, 1338, 1745, 1792, 1885
AjuI GAANNNNNNNTTGG 2 cut(s) 1421, 1453
AluBI AGCT 6 cut(s) 574, 593, 1322, 1451, 1556, 1591
AluI AGCT 6 cut(s) 574, 593, 1322, 1451, 1556, 1591
Alw21I GWGCWC 3 cut(s) 101, 365, 1501
Alw26I GTCTC 6 cut(s) 735, 1147, 1363, 1727, 1747, 1763
Alw44I GTGCAC 2 cut(s) 361, 1497
AlwI GGATC 1 cut(s) 959
Ama87I CYCGRG 2 cut(s) 308, 1373
AoxI GGCC 5 cut(s) 505, 620, 700, 1689, 1889
ApaLI GTGCAC 2 cut(s) 361, 1497
ApeKI GCWGC 5 cut(s) 17, 156, 262, 555, 1423
ApoI RAATTY 5 cut(s) 4, 42, 454, 1434, 1527
ArsI GACNNNNNNTTYG 2 cut(s) 1362, 1394
Asp700I GAANNNNTTC 1 cut(s) 1669
AspS9I GGNCC 6 cut(s) 505, 620, 787, 1268, 1689, 1889
AsuC2I CCSGG 1 cut(s) 1642
AsuHPI GGTGA 3 cut(s) 1138, 1361, 1715
AsuII TTCGAA 1 cut(s) 1833
AvaI CYCGRG 2 cut(s) 308, 1373
AvaII GGWCC 2 cut(s) 787, 1268
AxyI CCTNAGG 1 cut(s) 1264
BaeGI GKGCMC 2 cut(s) 365, 1501
BaeI ACNNNNGTAYC 2 cut(s) 296, 329
BanI GGYRCC 1 cut(s) 1004
BbrPI CACGTG 1 cut(s) 1159
BbsI GAAGAC 1 cut(s) 1061
Bbv12I GWGCWC 3 cut(s) 101, 365, 1501
BbvCI CCTCAGC 1 cut(s) 1458
BbvI GCAGC 5 cut(s) 4, 143, 274, 567, 1435
BccI CCATC 6 cut(s) 89, 364, 692, 836, 1153, 1474
BceAI ACGGC 1 cut(s) 687
BcgI CGANNNNNNTGC 2 cut(s) 1823, 1857
BciT130I CCWGG 6 cut(s) 203, 1122, 1340, 1747, 1794, 1887
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 1642
BcoDI GTCTC 6 cut(s) 735, 1147, 1363, 1727, 1747, 1763
BfaI CTAG 4 cut(s) 719, 909, 1319, 1698
BfmI CTRYAG 3 cut(s) 438, 639, 1780
BfuAI ACCTGC 3 cut(s) 731, 1822, 1849
BglII AGATCT 1 cut(s) 1315
BisI GCNGC 6 cut(s) 18, 157, 263, 556, 1424, 1818
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 6 cut(s) 19, 158, 264, 557, 1425, 1819
Bme1390I CCNGG 7 cut(s) 203, 1122, 1340, 1642, 1747, 1794, 1887
Bme18I GGWCC 2 cut(s) 787, 1268
BmeT110I CYCGRG 2 cut(s) 308, 1373
BmgT120I GGNCC 6 cut(s) 505, 620, 787, 1268, 1689, 1889
BmiI GGNNCC 3 cut(s) 207, 621, 1006
BmrFI CCNGG 7 cut(s) 203, 1122, 1340, 1642, 1747, 1794, 1887
BmrI ACTGGG 2 cut(s) 809, 926
BmsI GCATC 4 cut(s) 29, 1124, 1556, 1625
BmuI ACTGGG 2 cut(s) 809, 926
BpiI GAAGAC 1 cut(s) 1061
BpmI CTGGAG 4 cut(s) 665, 1527, 1729, 1776
Bpu10I CCTNAGC 3 cut(s) 321, 1086, 1458
Bpu1102I GCTNAGC 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 1833
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 1642
BsaAI YACGTR 1 cut(s) 1159
BsaI GGTCTC 2 cut(s) 1363, 1763
BsaJI CCNNGG 4 cut(s) 508, 1121, 1374, 1886
Bsc4I CCNNNNNNNGG 4 cut(s) 1557, 1558, 1698, 1752
Bse1I ACTGG 5 cut(s) 815, 822, 886, 932, 1692
Bse21I CCTNAGG 1 cut(s) 1264
Bse3DI GCAATG 2 cut(s) 118, 756
BseBI CCWGG 6 cut(s) 203, 1122, 1340, 1747, 1794, 1887
BseDI CCNNGG 4 cut(s) 508, 1121, 1374, 1886
BseGI GGATG 7 cut(s) 293, 368, 422, 550, 828, 1335, 1547
BseLI CCNNNNNNNGG 4 cut(s) 1557, 1558, 1698, 1752
BseMI GCAATG 2 cut(s) 118, 756
BseNI ACTGG 5 cut(s) 815, 822, 886, 932, 1692
BseRI GAGGAG 2 cut(s) 204, 1238
BseSI GKGCMC 2 cut(s) 365, 1501
BseXI GCAGC 5 cut(s) 4, 143, 274, 567, 1435
BseYI CCCAGC 1 cut(s) 1556
BshFI GGCC 5 cut(s) 507, 622, 702, 1691, 1891
BshNI GGYRCC 1 cut(s) 1004
BsiHKAI GWGCWC 3 cut(s) 101, 365, 1501
BsiHKCI CYCGRG 2 cut(s) 308, 1373
BsiSI CCGG 1 cut(s) 1641
BslI CCNNNNNNNGG 4 cut(s) 1557, 1558, 1698, 1752
BsmAI GTCTC 6 cut(s) 735, 1147, 1363, 1727, 1747, 1763
BsmI GAATGC 2 cut(s) 123, 474
BsnI GGCC 5 cut(s) 507, 622, 702, 1691, 1891
Bso31I GGTCTC 2 cut(s) 1363, 1763
BsoBI CYCGRG 2 cut(s) 308, 1373
Bsp119I TTCGAA 1 cut(s) 1833
Bsp1286I GDGCHC 3 cut(s) 101, 365, 1501
Bsp1407I TGTACA 1 cut(s) 1308
Bsp143I GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
Bsp1720I GCTNAGC 1 cut(s) 71
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 5 cut(s) 39, 389, 426, 1426, 1818
BspANI GGCC 5 cut(s) 507, 622, 702, 1691, 1891
BspLI GGNNCC 3 cut(s) 207, 621, 1006
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 3 cut(s) 731, 1822, 1849
BspPI GGATC 1 cut(s) 959
BspT104I TTCGAA 1 cut(s) 1833
BspT107I GGYRCC 1 cut(s) 1004
BspTNI GGTCTC 2 cut(s) 1363, 1763
BsrDI GCAATG 2 cut(s) 118, 756
BsrGI TGTACA 1 cut(s) 1308
BsrI ACTGG 5 cut(s) 815, 822, 886, 932, 1692
BssECI CCNNGG 4 cut(s) 508, 1121, 1374, 1886
BssMI GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
BssNAI GTATAC 1 cut(s) 1275
BssT1I CCWWGG 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 1275
Bst2UI CCWGG 6 cut(s) 203, 1122, 1340, 1747, 1794, 1887
Bst4CI ACNGT 7 cut(s) 403, 538, 879, 1105, 1571, 1775, 1784
Bst6I CTCTTC 4 cut(s) 439, 661, 1215, 1745
BstAPI GCANNNNNTGC 2 cut(s) 746, 1826
BstAUI TGTACA 1 cut(s) 1308
BstBAI YACGTR 1 cut(s) 1159
BstBI TTCGAA 1 cut(s) 1833
BstC8I GCNNGC 4 cut(s) 101, 591, 981, 1647
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 7 cut(s) 293, 368, 422, 550, 828, 1335, 1547
BstKTI GATC 6 cut(s) 28, 799, 967, 994, 1318, 1420
BstMAI GTCTC 6 cut(s) 735, 1147, 1363, 1727, 1747, 1763
BstMBI GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
BstNI CCWGG 6 cut(s) 203, 1122, 1340, 1747, 1794, 1887
BstNSI RCATGY 3 cut(s) 919, 983, 1309
BstSCI CCNGG 7 cut(s) 201, 1120, 1338, 1640, 1745, 1792, 1885
BstSFI CTRYAG 3 cut(s) 438, 639, 1780
BstSLI GKGCMC 2 cut(s) 365, 1501
BstV1I GCAGC 5 cut(s) 4, 143, 274, 567, 1435
BstV2I GAAGAC 1 cut(s) 1061
BstX2I RGATCY 1 cut(s) 1315
BstXI CCANNNNNNTGG 2 cut(s) 273, 944
BstYI RGATCY 1 cut(s) 1315
BstZ17I GTATAC 1 cut(s) 1275
Bsu36I CCTNAGG 1 cut(s) 1264
BsuRI GGCC 5 cut(s) 507, 622, 702, 1691, 1891
BtgI CCRYGG 1 cut(s) 508
BtgZI GCGATG 1 cut(s) 1101
BtsCI GGATG 7 cut(s) 293, 368, 422, 550, 828, 1335, 1547
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 4 cut(s) 87, 556, 1081, 1699
BveI ACCTGC 3 cut(s) 731, 1822, 1849
Cac8I GCNNGC 4 cut(s) 101, 591, 981, 1647
Cfr13I GGNCC 6 cut(s) 505, 620, 787, 1268, 1689, 1889
Csp6I GTAC 7 cut(s) 194, 313, 516, 1213, 1309, 1776, 1859
CspCI CAANNNNNGTGG 4 cut(s) 101, 136, 158, 193
CviAII CATG 9 cut(s) 298, 509, 559, 916, 923, 962, 980, 1306, 1603
CviQI GTAC 7 cut(s) 194, 313, 516, 1213, 1309, 1776, 1859
DpnI GATC 6 cut(s) 27, 798, 966, 993, 1317, 1419
DpnII GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 4 cut(s) 439, 661, 1215, 1745
EarI CTCTTC 4 cut(s) 439, 661, 1215, 1745
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 2 cut(s) 1363, 1763
Eco47I GGWCC 2 cut(s) 787, 1268
Eco57I CTGAAG 2 cut(s) 783, 1467
Eco72I CACGTG 1 cut(s) 1159
Eco81I CCTNAGG 1 cut(s) 1264
Eco88I CYCGRG 2 cut(s) 308, 1373
EcoO109I RGGNCCY 2 cut(s) 620, 787
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 6 cut(s) 201, 1120, 1338, 1745, 1792, 1885
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 2 cut(s) 981, 1307
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 9 cut(s) 301, 512, 562, 919, 926, 965, 983, 1309, 1606
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FatI CATG 9 cut(s) 297, 508, 558, 915, 922, 961, 979, 1305, 1602
FauI CCCGC 1 cut(s) 396
FbaI TGATCA 1 cut(s) 25
FblI GTMKAC 2 cut(s) 1242, 1274
Fnu4HI GCNGC 6 cut(s) 18, 157, 263, 556, 1424, 1818
FokI GGATG 7 cut(s) 280, 355, 409, 557, 815, 1322, 1534
Fsp4HI GCNGC 6 cut(s) 18, 157, 263, 556, 1424, 1818
FspBI CTAG 4 cut(s) 719, 909, 1319, 1698
GluI GCNGC 6 cut(s) 18, 157, 263, 556, 1424, 1818
GsaI CCCAGC 1 cut(s) 1560
GsuI CTGGAG 4 cut(s) 665, 1527, 1729, 1776
HaeIII GGCC 5 cut(s) 507, 622, 702, 1691, 1891
HapII CCGG 1 cut(s) 1641
Hin1II CATG 9 cut(s) 301, 512, 562, 919, 926, 965, 983, 1309, 1606
HincII GTYRAC 2 cut(s) 1184, 1808
HindII GTYRAC 2 cut(s) 1184, 1808
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 1 cut(s) 1641
HphI GGTGA 3 cut(s) 1138, 1361, 1715
Hpy166II GTNNAC 8 cut(s) 363, 937, 1184, 1198, 1243, 1275, 1499, 1808
Hpy188III TCNNGA 8 cut(s) 320, 406, 494, 542, 682, 772, 1088, 1256
Hpy8I GTNNAC 8 cut(s) 363, 937, 1184, 1198, 1243, 1275, 1499, 1808
HpyAV CCTTC 7 cut(s) 264, 343, 500, 557, 633, 758, 795
HpyCH4III ACNGT 7 cut(s) 403, 538, 879, 1105, 1571, 1775, 1784
HpyCH4IV ACGT 3 cut(s) 105, 1158, 1200
HpySE526I ACGT 3 cut(s) 105, 1158, 1200
Hsp92II CATG 9 cut(s) 301, 512, 562, 919, 926, 965, 983, 1309, 1606
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
LmnI GCTCC 1 cut(s) 1795
Lsp1109I GCAGC 5 cut(s) 4, 143, 274, 567, 1435
LweI GCATC 4 cut(s) 29, 1124, 1556, 1625
MaeI CTAG 4 cut(s) 719, 909, 1319, 1698
MaeII ACGT 3 cut(s) 105, 1158, 1200
MaeIII GTNAC 4 cut(s) 239, 532, 595, 1703
MalI GATC 6 cut(s) 27, 798, 966, 993, 1317, 1419
MboI GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
MboII GAAGA 8 cut(s) 76, 426, 678, 806, 1061, 1141, 1232, 1762
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 1 cut(s) 1315
MhlI GDGCHC 3 cut(s) 101, 365, 1501
MlyI GAGTC 6 cut(s) 403, 428, 678, 737, 762, 1037
MmeI TCCRAC 2 cut(s) 1009, 1183
Mph1103I ATGCAT 2 cut(s) 981, 1307
MroXI GAANNNNTTC 1 cut(s) 1669
MseI TTAA 2 cut(s) 783, 1535
MslI CAYNNNNRTG 2 cut(s) 1161, 1340
MspA1I CMGCKG 1 cut(s) 1426
MspI CCGG 1 cut(s) 1641
MspR9I CCNGG 7 cut(s) 203, 1122, 1340, 1642, 1747, 1794, 1887
MunI CAATTG 1 cut(s) 34
Mva1269I GAATGC 2 cut(s) 123, 474
MvaI CCWGG 6 cut(s) 203, 1122, 1340, 1747, 1794, 1887
NciI CCSGG 1 cut(s) 1642
NcoI CCATGG 1 cut(s) 508
NdeII GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
NlaIII CATG 9 cut(s) 301, 512, 562, 919, 926, 965, 983, 1309, 1606
NlaIV GGNNCC 3 cut(s) 207, 621, 1006
NmuCI GTSAC 4 cut(s) 239, 532, 595, 1703
NsiI ATGCAT 2 cut(s) 981, 1307
NspI RCATGY 3 cut(s) 919, 983, 1309
NspV TTCGAA 1 cut(s) 1833
PaeI GCATGC 1 cut(s) 983
PaqCI CACCTGC 3 cut(s) 731, 1822, 1849
PctI GAATGC 2 cut(s) 123, 474
PdmI GAANNNNTTC 1 cut(s) 1669
PfeI GAWTC 7 cut(s) 443, 497, 896, 901, 1022, 1259, 1665
PfoI TCCNGGA 2 cut(s) 1745, 1792
PkrI GCNGC 6 cut(s) 19, 158, 264, 557, 1425, 1819
PleI GAGTC 6 cut(s) 403, 427, 677, 736, 762, 1037
PmaCI CACGTG 1 cut(s) 1159
PmlI CACGTG 1 cut(s) 1159
PpsI GAGTC 6 cut(s) 403, 427, 677, 736, 762, 1037
Ppu21I YACGTR 1 cut(s) 1159
PpuMI RGGWCCY 1 cut(s) 787
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 6 cut(s) 201, 1120, 1338, 1745, 1792, 1885
PspCI CACGTG 1 cut(s) 1159
PspFI CCCAGC 1 cut(s) 1556
PspGI CCWGG 6 cut(s) 201, 1120, 1338, 1745, 1792, 1885
PspN4I GGNNCC 3 cut(s) 207, 621, 1006
PspPI GGNCC 6 cut(s) 505, 620, 787, 1268, 1689, 1889
PspPPI RGGWCCY 1 cut(s) 787
PstI CTGCAG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 1315
RsaI GTAC 7 cut(s) 195, 314, 517, 1214, 1310, 1777, 1860
RsaNI GTAC 7 cut(s) 194, 313, 516, 1213, 1309, 1776, 1859
RseI CAYNNNNRTG 2 cut(s) 1161, 1340
SaqAI TTAA 2 cut(s) 783, 1535
SatI GCNGC 6 cut(s) 18, 157, 263, 556, 1424, 1818
Sau3AI GATC 6 cut(s) 25, 796, 964, 991, 1315, 1417
Sau96I GGNCC 6 cut(s) 505, 620, 787, 1268, 1689, 1889
SchI GAGTC 6 cut(s) 403, 428, 678, 737, 762, 1037
ScrFI CCNGG 7 cut(s) 203, 1122, 1340, 1642, 1747, 1794, 1887
SduI GDGCHC 3 cut(s) 101, 365, 1501
SfaNI GCATC 4 cut(s) 29, 1124, 1556, 1625
SfcI CTRYAG 3 cut(s) 438, 639, 1780
SfuI TTCGAA 1 cut(s) 1833
SinI GGWCC 2 cut(s) 787, 1268
SmiMI CAYNNNNRTG 2 cut(s) 1161, 1340
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SphI GCATGC 1 cut(s) 983
SsiI CCGC 5 cut(s) 39, 389, 426, 1426, 1818
SspI AATATT 1 cut(s) 1489
SspMI CTAG 4 cut(s) 719, 909, 1319, 1698
StyD4I CCNGG 7 cut(s) 201, 1120, 1338, 1640, 1745, 1792, 1885
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 7 cut(s) 403, 538, 879, 1105, 1571, 1775, 1784
TaiI ACGT 3 cut(s) 108, 1161, 1203
TaqI TCGA 4 cut(s) 799, 862, 899, 1833
TatI WGTACW 1 cut(s) 1308
TauI GCSGC 1 cut(s) 1820
TfiI GAWTC 7 cut(s) 443, 497, 896, 901, 1022, 1259, 1665
Tru1I TTAA 2 cut(s) 783, 1535
Tru9I TTAA 2 cut(s) 783, 1535
TscAI CASTG 4 cut(s) 94, 556, 1081, 1699
TseFI GTSAC 4 cut(s) 239, 532, 595, 1703
TseI GCWGC 5 cut(s) 17, 156, 262, 555, 1423
Tsp45I GTSAC 4 cut(s) 239, 532, 595, 1703
TspDTI ATGAA 7 cut(s) 17, 282, 360, 939, 1006, 1344, 1637
TspGWI ACGGA 3 cut(s) 1260, 1534, 1751
TspRI CASTG 4 cut(s) 94, 556, 1081, 1699
VneI GTGCAC 2 cut(s) 361, 1497
VpaK11BI GGWCC 2 cut(s) 787, 1268
XapI RAATTY 5 cut(s) 4, 42, 454, 1434, 1527
XceI RCATGY 3 cut(s) 919, 983, 1309
XmiI GTMKAC 2 cut(s) 1242, 1274
XmnI GAANNNNTTC 1 cut(s) 1669
XspI CTAG 4 cut(s) 719, 909, 1319, 1698
Zsp2I ATGCAT 2 cut(s) 981, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.