Rroxscaffold_1G00058880

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000360
Physical Location & Seq
Reverse (-)
80835983 .. 80840651
4669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058880.1

Sequence Viewer

Length: 1932 bp
ATGGGTTCCGTGTTGAAGAAGCTGAAGAAGGCTCTCACTCCGAAGTTGCGCTGCGACTCCTCCTCCACCGCAACCTCTCCGCCTCGCCTCTCCGAAACGACGTCGTCAGAGTTCTCCACAACATCTTCCAGCTTCTCCCGCGAGGATTGGCAAAAGCAAGGATTTTGCCTTGGTTGGCAACCCCTATCCAAGAAATGTTGCACCATTTGCCTCAGTAACGTGAAAAGAGGGCAAGGTCAAGCCATCTTCACTGCTGAGTGTTCCCACTCTTTCCACTTCGACTGCATTGCCAAAAATGTCGAGCATGGAAATCTTTGCTGCCCTATCTGCAGGGCCAAATGGGATAGAATTAATGTACCGTTCCAAGCTCCCAATGATAGTGTGGATCCTCAGCGAAACCACTTGGGCAGTATCCATCCTAATCCTGAGCCCTTCTCTGACTATGAGCCTTTCCTTACCACTTCTTTAACTCAGTCATCTGTCTCCCAGAGTGTGACAATTAAGATTCACACAGAGTCACCTGCGATCTCTGCTTCAGAATCACGCGCAAATTTTCCTGTGCTTGTCAGCATTCGTGCACCATCTCTTGAGGACCCTAATGGCCACAGTCATACACCCGTTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGGGTGAAACAAAGCTTTCCCTTGTCAAGAGTGCTGTCAAATTTCTCATCCAAAACTTGGGGCCTTCAGACCGGCTGTCTATAGTAACATTCTCAACAACTGCTACAAGGGTGTTTCCTCTACGAAGAATGTCTGATGATGGCCGTGTGAGTGCCATCCTAGCTGTTGAATCGCTTAGATCAAGTGGTGGAACTAGCATTGCGGAAGGACTCAAGAAAGGAACTCAAGTCCTTGAAGAACGAAGGGAAAGGAACCCAGTTTCTAGCATCATGCTCTTATCCGATGGCATCGATTCATACTATCCAGGTGGAAGCCAACTGAAGAACCAATTGCCTGCCTCTATTTTTTCTAGCAAAACAGAACATGGAATTCCAGTCCACACATTTGGGTTTGGCAAGGACCATTGTCCAACTACTTTGCATGCTATATCTGATGCATCAGGCGGTACCTTTTCCTTCATTGAATCAGTTGAGATGATACAAGATTCCTTCGCTATATGCATTGGTGGTCTTCTCAGTGTTGTGACTCAAGAATTGCGACTGACACTCAAGTCAGCAACACGTGGGGTGATGATTGTAGCAATACCATCAGGGAGATATTTCAGTGAGATTTCTGACGAGGGCAAGCAAGGTGTTGTTACTGTTGGAAATATGTACGCAGAAGAGGAGAAGCAATTTCTGGTATACCTCTTTGTTCCGAAATTCTCAAGAAGTGCCACCAAAACATCATTGTTGGAGGTGTTATGTGTGTATAAAGATCTAGCTGCAAATGAGTTGATTCAGGTTCCAGGTGAAGGAATAGAAATACTGAGACCTGAGGTTTGTTCCCCTGCTGAAAAGGCAGTGTCTTTGGAGGTTGATCGGCAGTGCAACAGAGTTTCAGTCGCTGAAGCTATTGCAGAGGCACAACGGATGGCTGAGATGGGAAATCTGAAGGGTGCAAAGGCGACTTTGGCTCAAGAAAGATCAACTCTCTTGTCATCACCAGCTGCCCAAGCCGGGGACAGCCTCATTACTTTGCTTGAAGCTCAGCTCACTGAAGTCATTCATAGAATGGCAACAATGGATTTGTATACGCTGACCGGCCGTGCTTACACTCTCTCAGAAATGAGCTCGCACTCATTGCAAAGAGCCGCAGCTACAGGTGACTCGACAACCTGGAGTCTCACCATGAGTGAAGGTGGAAATTCTGCCACCAGTAGTGGGGTGTTTGAAACACCTCATATGAGCAGAATGCTGAGGTTATCGCACAATCCTGGGAATAATCCTGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA VWA_3 Vwaint zf-RING_11

Protein Analysis

643

Amino Acids

69.44

Weight (kDa)

6.48

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 66 - 111 3.7e-06 Ring finger domain
zf-RING_11 PF17123 67 - 96 7.4e-10 RING-like zinc finger
zf-C3HC4 PF00097 67 - 110 2.7e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 202 - 321 2.9e-09 VWA domain containing CoxE-like protein
VWA PF00092 208 - 376 1e-22 von Willebrand factor type A domain
VWA_3 PF13768 208 - 371 3.4e-16 von Willebrand factor type A domain
VWA_2 PF13519 209 - 314 4.6e-18 von Willebrand factor type A domain
WAV3_C PF25243 514 - 570 9.3e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 555 - 631 4.2e-09 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
AasI GACNNNNNNGTC 1 cut(s) 1204
AatII GACGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 529
Acc65I GGTACC 1 cut(s) 1100
AccB1I GGYRCC 1 cut(s) 1100
AccB7I CCANNNNNTGG 1 cut(s) 712
AccI GTMKAC 2 cut(s) 1338, 1727
AccII CGCG 2 cut(s) 141, 546
AciI CCGC 6 cut(s) 69, 80, 139, 857, 1098, 1788
AclWI GGATC 2 cut(s) 380, 393
AcoI YGGCCR 3 cut(s) 601, 796, 1738
AcsI RAATTY 5 cut(s) 550, 695, 1023, 1355, 1840
AcuI CTGAAG 7 cut(s) 44, 519, 705, 995, 1563, 1607, 1713
AcvI CACGTG 1 cut(s) 1217
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 3 cut(s) 357, 1102, 1310
AfiI CCNNNNNNNGG 5 cut(s) 712, 1448, 1653, 1654, 1857
AflIII ACRYGT 1 cut(s) 1214
AgsI TTSAA 6 cut(s) 16, 824, 890, 1118, 1679, 1868
AhdI GACNNNNNGTC 1 cut(s) 730
AjnI CCWGG 4 cut(s) 958, 1441, 1811, 1909
Alw21I GWGCWC 2 cut(s) 580, 1769
Alw26I GTCTC 3 cut(s) 487, 1459, 1823
Alw44I GTGCAC 1 cut(s) 576
AlwI GGATC 2 cut(s) 380, 393
AlwNI CAGNNNCTG 1 cut(s) 1541
AoxI GGCC 5 cut(s) 333, 601, 716, 796, 1738
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 7 cut(s) 51, 318, 651, 1418, 1643, 1790, 1925
ApoI RAATTY 5 cut(s) 550, 695, 1023, 1355, 1840
ArsI GACNNNNNNTTYG 2 cut(s) 1458, 1490
AseI ATTAAT 1 cut(s) 351
Asp700I GAANNNNTTC 1 cut(s) 1698
Asp718I GGTACC 1 cut(s) 1100
AspLEI GCGC 2 cut(s) 51, 548
AspS9I GGNCC 4 cut(s) 333, 592, 716, 1054
AsuC2I CCSGG 1 cut(s) 1654
AsuHPI GGTGA 7 cut(s) 510, 671, 1234, 1457, 1629, 1811, 1813
AvaII GGWCC 2 cut(s) 592, 1054
AxyI CCTNAGG 1 cut(s) 1470
BaeGI GKGCMC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 1100
BanII GRGCYC 2 cut(s) 432, 1769
BbrPI CACGTG 1 cut(s) 1217
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 2 cut(s) 580, 1769
BbvCI CCTCAGC 2 cut(s) 390, 1892
BbvI GCAGC 6 cut(s) 38, 305, 663, 1405, 1630, 1802
BccI CCATC 9 cut(s) 251, 423, 589, 788, 818, 932, 1249, 1561, 1570
BceAI ACGGC 2 cut(s) 783, 1725
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 4 cut(s) 960, 1443, 1813, 1911
BciVI GTATCC 1 cut(s) 422
BcnI CCSGG 1 cut(s) 1654
BcoDI GTCTC 3 cut(s) 487, 1459, 1823
BfaI CTAG 5 cut(s) 815, 849, 918, 1005, 1415
BfmI CTRYAG 4 cut(s) 328, 735, 1794, 1923
BfuAI ACCTGC 1 cut(s) 529
BfuI GTATCC 1 cut(s) 422
BglII AGATCT 1 cut(s) 1411
BisI GCNGC 8 cut(s) 52, 319, 652, 1419, 1644, 1788, 1791, 1926
BlpI GCTNAGC 1 cut(s) 1683
BlsI GCNGC 8 cut(s) 53, 320, 653, 1420, 1645, 1789, 1792, 1927
Bme1390I CCNGG 5 cut(s) 960, 1443, 1654, 1813, 1911
Bme18I GGWCC 2 cut(s) 592, 1054
BmeRI GACNNNNNGTC 1 cut(s) 730
BmgT120I GGNCC 4 cut(s) 333, 592, 716, 1054
BmiI GGNNCC 7 cut(s) 7, 387, 594, 717, 908, 1102, 1440
BmrFI CCNGG 5 cut(s) 960, 1443, 1654, 1813, 1911
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 4 cut(s) 930, 951, 1078, 1100
BmuI ACTGGG 1 cut(s) 905
BoxI GACNNNNGTC 1 cut(s) 1059
BpiI GAAGAC 1 cut(s) 1157
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpmI CTGGAG 1 cut(s) 1834
Bpu10I CCTNAGC 3 cut(s) 390, 426, 1892
Bpu1102I GCTNAGC 1 cut(s) 1683
BpuEI CTTGAG 7 cut(s) 608, 851, 864, 1167, 1187, 1345, 1596
BpuMI CCSGG 1 cut(s) 1654
Bsa29I ATCGAT 1 cut(s) 945
BsaAI YACGTR 1 cut(s) 1217
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 1459
BsaJI CCNNGG 3 cut(s) 169, 1653, 1910
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 5 cut(s) 712, 1448, 1653, 1654, 1857
Bse118I RCCGGY 2 cut(s) 726, 1736
Bse1I ACTGG 3 cut(s) 911, 1028, 1851
Bse21I CCTNAGG 1 cut(s) 1470
Bse3DI GCAATG 3 cut(s) 285, 852, 1775
BseBI CCWGG 4 cut(s) 960, 1443, 1813, 1911
BseCI ATCGAT 1 cut(s) 945
BseDI CCNNGG 3 cut(s) 169, 1653, 1910
BseGI GGATG 5 cut(s) 415, 646, 702, 810, 1572
BseLI CCNNNNNNNGG 5 cut(s) 712, 1448, 1653, 1654, 1857
BseMI GCAATG 3 cut(s) 285, 852, 1775
BseNI ACTGG 3 cut(s) 911, 1028, 1851
BseRI GAGGAG 3 cut(s) 49, 52, 1334
BseSI GKGCMC 1 cut(s) 580
BseX3I CGGCCG 1 cut(s) 1738
BseXI GCAGC 6 cut(s) 38, 305, 663, 1405, 1630, 1802
Bsh1236I CGCG 2 cut(s) 141, 546
Bsh1285I CGRYCG 1 cut(s) 1741
BshFI GGCC 5 cut(s) 335, 603, 718, 798, 1740
BshNI GGYRCC 1 cut(s) 1100
BshVI ATCGAT 1 cut(s) 945
BsiEI CGRYCG 1 cut(s) 1741
BsiHKAI GWGCWC 2 cut(s) 580, 1769
BsiSI CCGG 3 cut(s) 727, 1653, 1737
BslFI GGGAC 1 cut(s) 1670
BslI CCNNNNNNNGG 5 cut(s) 712, 1448, 1653, 1654, 1857
BsmAI GTCTC 3 cut(s) 487, 1459, 1823
BsmFI GGGAC 1 cut(s) 1670
BsmI GAATGC 2 cut(s) 570, 1893
BsnI GGCC 5 cut(s) 335, 603, 718, 798, 1740
Bso31I GGTCTC 1 cut(s) 1459
Bsp1286I GDGCHC 3 cut(s) 432, 580, 1769
Bsp143I GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
Bsp1720I GCTNAGC 1 cut(s) 1683
BspACI CCGC 6 cut(s) 69, 80, 139, 857, 1098, 1788
BspANI GGCC 5 cut(s) 335, 603, 718, 798, 1740
BspDI ATCGAT 1 cut(s) 945
BspFNI CGCG 2 cut(s) 141, 546
BspLI GGNNCC 7 cut(s) 7, 387, 594, 717, 908, 1102, 1440
BspMAI CTGCAG 2 cut(s) 332, 1927
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 380, 393
BspT107I GGYRCC 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 1459
BsrDI GCAATG 3 cut(s) 285, 852, 1775
BsrFI RCCGGY 2 cut(s) 726, 1736
BsrI ACTGG 3 cut(s) 911, 1028, 1851
BssAI RCCGGY 2 cut(s) 726, 1736
BssECI CCNNGG 3 cut(s) 169, 1653, 1910
BssMI GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
BssNAI GTATAC 2 cut(s) 1339, 1728
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 169
Bst1107I GTATAC 2 cut(s) 1339, 1728
Bst2UI CCWGG 4 cut(s) 960, 1443, 1813, 1911
Bst4CI ACNGT 4 cut(s) 360, 608, 634, 1297
Bst6I CTCTTC 1 cut(s) 1311
BstACI GRCGYC 1 cut(s) 101
BstAPI GCANNNNNTGC 2 cut(s) 207, 1777
BstBAI YACGTR 1 cut(s) 1217
BstC8I GCNNGC 4 cut(s) 990, 1077, 1280, 1769
BstF5I GGATG 5 cut(s) 415, 646, 702, 810, 1572
BstFNI CGCG 2 cut(s) 141, 546
BstHHI GCGC 2 cut(s) 51, 548
BstKTI GATC 6 cut(s) 388, 528, 836, 1414, 1516, 1622
BstMAI GTCTC 3 cut(s) 487, 1459, 1823
BstMBI GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
BstMCI CGRYCG 1 cut(s) 1741
BstMWI GCNNNNNNNGC 9 cut(s) 138, 207, 327, 530, 815, 1493, 1607, 1649, 1777
BstNI CCWGG 4 cut(s) 960, 1443, 1813, 1911
BstNSI RCATGY 1 cut(s) 1079
BstPAI GACNNNNGTC 1 cut(s) 1059
BstSCI CCNGG 5 cut(s) 958, 1441, 1652, 1811, 1909
BstSFI CTRYAG 4 cut(s) 328, 735, 1794, 1923
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 2 cut(s) 141, 546
BstV1I GCAGC 6 cut(s) 38, 305, 663, 1405, 1630, 1802
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 2 cut(s) 385, 1411
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 2 cut(s) 385, 1411
BstZ17I GTATAC 2 cut(s) 1339, 1728
BstZI CGGCCG 1 cut(s) 1738
Bsu15I ATCGAT 1 cut(s) 945
Bsu36I CCTNAGG 1 cut(s) 1470
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 5 cut(s) 335, 603, 718, 798, 1740
BsuTUI ATCGAT 1 cut(s) 945
BtsCI GGATG 5 cut(s) 415, 646, 702, 810, 1572
BtsI GCAGTG 3 cut(s) 249, 1503, 1526
BtsIMutI CAGTG 6 cut(s) 249, 1177, 1264, 1503, 1526, 1689
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 4 cut(s) 990, 1077, 1280, 1769
CaiI CAGNNNCTG 1 cut(s) 1541
CfoI GCGC 2 cut(s) 51, 548
Cfr10I RCCGGY 2 cut(s) 726, 1736
Cfr13I GGNCC 4 cut(s) 333, 592, 716, 1054
ClaI ATCGAT 1 cut(s) 945
Csp6I GTAC 3 cut(s) 356, 1101, 1309
CviAII CATG 7 cut(s) 305, 655, 925, 1019, 1076, 1825, 1929
CviQI GTAC 3 cut(s) 356, 1101, 1309
DpnI GATC 6 cut(s) 387, 527, 835, 1413, 1515, 1621
DpnII GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
DrdI GACNNNNNNGTC 1 cut(s) 1204
DriI GACNNNNNGTC 1 cut(s) 730
DseDI GACNNNNNNGTC 1 cut(s) 1204
EaeI YGGCCR 3 cut(s) 601, 796, 1738
EagI CGGCCG 1 cut(s) 1738
Eam1104I CTCTTC 1 cut(s) 1311
Eam1105I GACNNNNNGTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 1311
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 1767
EclXI CGGCCG 1 cut(s) 1738
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 432, 1769
Eco31I GGTCTC 1 cut(s) 1459
Eco47I GGWCC 2 cut(s) 592, 1054
Eco52I CGGCCG 1 cut(s) 1738
Eco53kI GAGCTC 1 cut(s) 1767
Eco57I CTGAAG 7 cut(s) 44, 519, 705, 995, 1563, 1607, 1713
Eco72I CACGTG 1 cut(s) 1217
Eco81I CCTNAGG 1 cut(s) 1470
EcoICRI GAGCTC 1 cut(s) 1767
EcoO109I RGGNCCY 2 cut(s) 592, 716
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 4 cut(s) 958, 1441, 1811, 1909
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 2 cut(s) 1093, 1157
EcoT38I GRGCYC 2 cut(s) 432, 1769
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 7 cut(s) 308, 658, 928, 1022, 1079, 1828, 1932
FaqI GGGAC 1 cut(s) 1670
FatI CATG 7 cut(s) 304, 654, 924, 1018, 1075, 1824, 1928
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 1878
FblI GTMKAC 2 cut(s) 1338, 1727
Fnu4HI GCNGC 8 cut(s) 52, 319, 652, 1419, 1644, 1788, 1791, 1926
FokI GGATG 5 cut(s) 402, 653, 689, 797, 1579
FriOI GRGCYC 2 cut(s) 432, 1769
Fsp4HI GCNGC 8 cut(s) 52, 319, 652, 1419, 1644, 1788, 1791, 1926
FspBI CTAG 5 cut(s) 815, 849, 918, 1005, 1415
GlaI GCGC 2 cut(s) 50, 547
GluI GCNGC 8 cut(s) 52, 319, 652, 1419, 1644, 1788, 1791, 1926
GsuI CTGGAG 1 cut(s) 1834
HaeIII GGCC 5 cut(s) 335, 603, 718, 798, 1740
HapII CCGG 3 cut(s) 727, 1653, 1737
HhaI GCGC 2 cut(s) 51, 548
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 7 cut(s) 308, 658, 928, 1022, 1079, 1828, 1932
Hin6I GCGC 2 cut(s) 49, 546
HinP1I GCGC 2 cut(s) 49, 546
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 668
HpaII CCGG 3 cut(s) 727, 1653, 1737
HphI GGTGA 7 cut(s) 510, 671, 1234, 1457, 1629, 1811, 1813
Hpy166II GTNNAC 5 cut(s) 578, 622, 1033, 1339, 1728
Hpy188III TCNNGA 8 cut(s) 425, 587, 638, 682, 868, 1184, 1362, 1613
Hpy8I GTNNAC 5 cut(s) 578, 622, 1033, 1339, 1728
Hpy99I CGWCG 2 cut(s) 103, 106
HpyCH4III ACNGT 4 cut(s) 360, 608, 634, 1297
HpyCH4IV ACGT 3 cut(s) 101, 219, 1216
HpyF10VI GCNNNNNNNGC 9 cut(s) 138, 207, 327, 530, 815, 1493, 1607, 1649, 1777
HpySE526I ACGT 3 cut(s) 101, 219, 1216
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 7 cut(s) 308, 658, 928, 1022, 1079, 1828, 1932
HspAI GCGC 2 cut(s) 49, 546
KpnI GGTACC 1 cut(s) 1104
Kzo9I GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 6 cut(s) 38, 305, 663, 1405, 1630, 1802
LweI GCATC 4 cut(s) 930, 951, 1078, 1100
MaeI CTAG 5 cut(s) 815, 849, 918, 1005, 1415
MaeII ACGT 3 cut(s) 101, 219, 1216
MaeIII GTNAC 8 cut(s) 215, 493, 516, 628, 739, 1177, 1291, 1799
MalI GATC 6 cut(s) 387, 527, 835, 1413, 1515, 1621
MboI GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
MboII GAAGA 9 cut(s) 28, 37, 117, 238, 792, 902, 988, 1157, 1328
MfeI CAATTG 1 cut(s) 983
MflI RGATCY 2 cut(s) 385, 1411
MhlI GDGCHC 3 cut(s) 432, 580, 1769
MlsI TGGCCA 1 cut(s) 603
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 6 cut(s) 50, 524, 858, 1174, 1796, 1825
MmeI TCCRAC 3 cut(s) 1088, 1279, 1368
Mox20I TGGCCA 1 cut(s) 603
Mph1103I ATGCAT 2 cut(s) 1093, 1157
MroXI GAANNNNTTC 1 cut(s) 1698
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 351, 467, 501
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 1643
MspI CCGG 3 cut(s) 727, 1653, 1737
MspR9I CCNGG 5 cut(s) 960, 1443, 1654, 1813, 1911
MunI CAATTG 1 cut(s) 983
Mva1269I GAATGC 2 cut(s) 570, 1893
MvaI CCWGG 4 cut(s) 960, 1443, 1813, 1911
MvnI CGCG 2 cut(s) 141, 546
MwoI GCNNNNNNNGC 9 cut(s) 138, 207, 327, 530, 815, 1493, 1607, 1649, 1777
NciI CCSGG 1 cut(s) 1654
NdeI CATATG 1 cut(s) 1878
NdeII GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
NlaIII CATG 7 cut(s) 308, 658, 928, 1022, 1079, 1828, 1932
NlaIV GGNNCC 7 cut(s) 7, 387, 594, 717, 908, 1102, 1440
NmuCI GTSAC 4 cut(s) 493, 516, 1177, 1799
NsiI ATGCAT 2 cut(s) 1093, 1157
NspI RCATGY 1 cut(s) 1079
PaeI GCATGC 1 cut(s) 1079
PaqCI CACCTGC 1 cut(s) 529
PctI GAATGC 2 cut(s) 570, 1893
PdmI GAANNNNTTC 1 cut(s) 1698
PfeI GAWTC 7 cut(s) 505, 539, 824, 947, 1118, 1139, 1432
PflFI GACNNNGTC 1 cut(s) 103
PflMI CCANNNNNTGG 1 cut(s) 712
PkrI GCNGC 8 cut(s) 53, 320, 653, 1420, 1645, 1789, 1792, 1927
PleI GAGTC 6 cut(s) 50, 523, 858, 1174, 1796, 1824
PmaCI CACGTG 1 cut(s) 1217
PmlI CACGTG 1 cut(s) 1217
PpsI GAGTC 6 cut(s) 50, 523, 858, 1174, 1796, 1824
Ppu21I YACGTR 1 cut(s) 1217
PpuMI RGGWCCY 1 cut(s) 592
PshAI GACNNNNGTC 1 cut(s) 1059
PshBI ATTAAT 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 1769
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 4 cut(s) 958, 1441, 1811, 1909
PspCI CACGTG 1 cut(s) 1217
PspGI CCWGG 4 cut(s) 958, 1441, 1811, 1909
PspN4I GGNNCC 7 cut(s) 7, 387, 594, 717, 908, 1102, 1440
PspPI GGNCC 4 cut(s) 333, 592, 716, 1054
PspPPI RGGWCCY 1 cut(s) 592
PstI CTGCAG 2 cut(s) 332, 1927
PstNI CAGNNNCTG 1 cut(s) 1541
PsuI RGATCY 2 cut(s) 385, 1411
PsyI GACNNNGTC 1 cut(s) 103
PvuII CAGCTG 1 cut(s) 1643
RsaI GTAC 3 cut(s) 357, 1102, 1310
RsaNI GTAC 3 cut(s) 356, 1101, 1309
SacI GAGCTC 1 cut(s) 1769
SaqAI TTAA 3 cut(s) 351, 467, 501
SatI GCNGC 8 cut(s) 52, 319, 652, 1419, 1644, 1788, 1791, 1926
Sau3AI GATC 6 cut(s) 385, 525, 833, 1411, 1513, 1619
Sau96I GGNCC 4 cut(s) 333, 592, 716, 1054
SchI GAGTC 6 cut(s) 50, 524, 858, 1174, 1796, 1825
ScrFI CCNGG 5 cut(s) 960, 1443, 1654, 1813, 1911
SduI GDGCHC 3 cut(s) 432, 580, 1769
SfaNI GCATC 4 cut(s) 930, 951, 1078, 1100
SfcI CTRYAG 4 cut(s) 328, 735, 1794, 1923
SinI GGWCC 2 cut(s) 592, 1054
SmlI CTYRAG 7 cut(s) 587, 866, 879, 1182, 1202, 1360, 1611
SmoI CTYRAG 7 cut(s) 587, 866, 879, 1182, 1202, 1360, 1611
SphI GCATGC 1 cut(s) 1079
SsiI CCGC 6 cut(s) 69, 80, 139, 857, 1098, 1788
SspMI CTAG 5 cut(s) 815, 849, 918, 1005, 1415
SstI GAGCTC 1 cut(s) 1769
StyD4I CCNGG 5 cut(s) 958, 1441, 1652, 1811, 1909
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 4 cut(s) 360, 608, 634, 1297
TaiI ACGT 3 cut(s) 104, 222, 1219
TaqI TCGA 4 cut(s) 279, 300, 945, 1805
TauI GCSGC 1 cut(s) 1790
TfiI GAWTC 7 cut(s) 505, 539, 824, 947, 1118, 1139, 1432
Tru1I TTAA 3 cut(s) 351, 467, 501
Tru9I TTAA 3 cut(s) 351, 467, 501
TscAI CASTG 6 cut(s) 256, 1177, 1264, 1503, 1526, 1696
TseFI GTSAC 4 cut(s) 493, 516, 1177, 1799
TseI GCWGC 7 cut(s) 51, 318, 651, 1418, 1643, 1790, 1925
Tsp45I GTSAC 4 cut(s) 493, 516, 1177, 1799
TspDTI ATGAA 3 cut(s) 939, 1102, 1691
TspGWI ACGGA 1 cut(s) 1579
TspRI CASTG 6 cut(s) 256, 1177, 1264, 1503, 1526, 1696
Tth111I GACNNNGTC 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 712
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 2 cut(s) 592, 1054
VspI ATTAAT 1 cut(s) 351
XapI RAATTY 5 cut(s) 550, 695, 1023, 1355, 1840
XceI RCATGY 1 cut(s) 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XmiI GTMKAC 2 cut(s) 1338, 1727
XmnI GAANNNNTTC 1 cut(s) 1698
XspI CTAG 5 cut(s) 815, 849, 918, 1005, 1415
ZraI GACGTC 1 cut(s) 102
Zsp2I ATGCAT 2 cut(s) 1093, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.