RLG00000032423

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000360
Physical Location & Seq
Forward (+)
15038092 .. 15040532
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032423

Sequence Viewer

Length: 1839 bp
ATGAATTCGTCTGAGGCAGCATCTGATCAAAACAATTGTGGCATTTGCAGGAGTAGCCTGACAACAGAGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTGTTTCCACTACCCTTGTATAGCCAACAATGTCCAGCATGGAAATCTTTGCTGCCCCATCTACCGAGCAAAGTGGAATAAGGATAATGTGCCTTTCCAAGTTCCTCCTCCTCAAAACCATAACTCGGCTGCGATCCCTCCTCACATCCCATTCCCATCGCAGCAGCTACCCCGTTGGTATCAGTACCCATCCATGCAGGAACCCGTGTACCCTCCTGAGCCACCTACCTTCTCTGACGATGAACCTCTCCAATCGTCAACCTCACCTATTCAATCCCTTGTCCCCCAGAAAATAACACTTAAGACTCATACAGAGTCCTCTTCTATCTCTGCGGCAGAATCAAGCCCAAATTTTCCTGTCCTTGTCAGTATTCGTGCACCACAACTTAATGATTCTGAAGGCCATGGTCGTACGCGCATTGACCTCGTAACAGTTTTAGACGTAAGTGGCAGCATGCAAGGCACAAAGATTTCCCTTCTCAAGCAAGCTGTCACATTTGTCACAGAAAACTTGGGGCCTTCGGATAGACTTTCTATTGTTTCGTTCTCATCAAGTTCTAGAAGAGTCTTTCCTCTCCAGAGAATGTCCGTTGATGGTCGTGAAAGTGCCATCCGAGCCATTGAGTCTCTTAGAGCAGGTGGGGCAACTAACATTGTAGAAGGACTCAAGAAAGGAACTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGCATCATCCTCTTATCTGATGGCAAAGATACATACAACAGAACTGCAAACCGACTGTTGAACCAGTTGCCTGATTCGATTCGTTCTAGCAACACGCAACAGGAAACCCCAGTCCACACATTTGGATTTGGCTACGATCATGATCCCAACATTATGCATGCTATATCTGATGTGTCAGGTGGCACCTTTTCGTTCATTGAATCAGTTGAAATGATACAAGACTCCTTTGCTCTGTGTATTGGGGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCACCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACATGTGAATGAGATTTCTACTGAGGGTCAGCAAGGTGTTGTTCATGTTGGAAATATGTATGCTGAAGAGGAAAAACAATTTCTGGTCTACCTAACAGTTCCAGAATCGTCAGCTCCGCAGGCGCATAATAAGACGTCATTGTTAGAGGTGCAAGGTGAGAAAATAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCTGAAAAGACAGTTTCTTTGGAGGTTGATCGGCAGCGGAACAGAATATTGGTGGCTGAAGCTATATCTGAGGCACAAAGGTTGGCTGAGATTGGAAATCTGGAGGGTGCACAGGCTCTTTTGGCTCAACGGAAAGAAATTATCTCAACATCAACAGCAGCCCAAGCAGGGGATTTTCACAGTAATTTGTTTGAAACTGAGCTTAGAGAAATGATGGATAGAATGGCAAGTATGAAGTTGTATAAACGAACCGGGCGTGCTTACGCTCTCTCAGGACTCAGTTCCCATAAGTTGCAGAGGGCCACAGCTAGGAAGTGTCGCAGGGTTTCCCATAGTGGGGGCTTAGAAGGTCGCAAGGCTTCCGGTGCTTCATCAGGTGCTACTTCAGGTGAATCGCCTGCCGCCGTTGGTGCTTTTGAAACACCTGCTATGGTCAGAATGGTAGAAAAATCACAGAAGAGGAATCAACCTGCGCCAGACCAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

613

Amino Acids

66.73

Weight (kDa)

6.78

Isoelectric Point (pI)

61.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_11 PF17123 12 - 42 6.5e-08 RING-like zinc finger
VWA_CoxE PF05762 173 - 289 9.7e-07 VWA domain containing CoxE-like protein
VWA PF00092 176 - 344 9.7e-22 von Willebrand factor type A domain
VWA_3 PF13768 176 - 339 1.6e-17 von Willebrand factor type A domain
VWA_2 PF13519 177 - 283 9.5e-19 von Willebrand factor type A domain
Vwaint PF14624 516 - 603 2.2e-12 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 731, 1783
AatII GACGTC 1 cut(s) 1292
Acc36I ACCTGC 3 cut(s) 731, 1783, 1828
AccB1I GGYRCC 1 cut(s) 1004
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 1 cut(s) 520
AciI CCGC 4 cut(s) 437, 1271, 1387, 1752
AclWI GGATC 2 cut(s) 232, 959
AcsI RAATTY 2 cut(s) 4, 454
AcuI CTGAAG 4 cut(s) 522, 1239, 1428, 1719
AcyI GRCGYC 1 cut(s) 1289
AfaI GTAC 3 cut(s) 290, 314, 517
AfiI CCNNNNNNNGG 6 cut(s) 229, 1518, 1519, 1659, 1686, 1687
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 1156
AgsI TTSAA 7 cut(s) 377, 794, 883, 1022, 1031, 1544, 1769
AjnI CCWGG 1 cut(s) 1120
AjuI GAANNNNNNNTTGG 2 cut(s) 1382, 1414
AluBI AGCT 6 cut(s) 271, 593, 1268, 1412, 1552, 1658
AluI AGCT 6 cut(s) 271, 593, 1268, 1412, 1552, 1658
Alw21I GWGCWC 3 cut(s) 101, 484, 1462
Alw26I GTCTC 3 cut(s) 735, 1147, 1324
Alw44I GTGCAC 2 cut(s) 480, 1458
AlwI GGATC 2 cut(s) 232, 959
AlwNI CAGNNNCTG 1 cut(s) 23
AoxI GGCC 4 cut(s) 73, 505, 620, 1650
ApaLI GTGCAC 2 cut(s) 480, 1458
ApeKI GCWGC 8 cut(s) 17, 156, 233, 265, 268, 555, 1384, 1508
ApoI RAATTY 2 cut(s) 4, 454
ArsI GACNNNNNNTTYG 4 cut(s) 1323, 1351, 1355, 1383
AspLEI GCGC 3 cut(s) 522, 1279, 1825
AspS9I GGNCC 3 cut(s) 620, 787, 1650
AsuC2I CCSGG 1 cut(s) 1603
AsuHPI GGTGA 5 cut(s) 360, 1110, 1138, 1322, 1751
AvaII GGWCC 1 cut(s) 787
AxyI CCTNAGG 1 cut(s) 1335
BaeGI GKGCMC 2 cut(s) 484, 1462
BaeI ACNNNNGTAYC 2 cut(s) 296, 329
BanI GGYRCC 1 cut(s) 1004
BbsI GAAGAC 1 cut(s) 1061
Bbv12I GWGCWC 3 cut(s) 101, 484, 1462
BbvI GCAGC 8 cut(s) 29, 143, 220, 277, 280, 567, 1396, 1520
BccI CCATC 9 cut(s) 89, 170, 268, 301, 692, 722, 836, 1153, 1558
BceAI ACGGC 1 cut(s) 1739
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 1 cut(s) 1122
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 1603
BcoDI GTCTC 3 cut(s) 735, 1147, 1324
BfaI CTAG 3 cut(s) 663, 909, 1659
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 3 cut(s) 731, 1783, 1828
Bme1390I CCNGG 2 cut(s) 1122, 1603
Bme18I GGWCC 1 cut(s) 787
BmgT120I GGNCC 3 cut(s) 620, 787, 1650
BmiI GGNNCC 3 cut(s) 306, 621, 1006
BmrFI CCNGG 2 cut(s) 1122, 1603
BmrI ACTGGG 2 cut(s) 809, 926
BmsI GCATC 3 cut(s) 29, 834, 1124
BmuI ACTGGG 2 cut(s) 809, 926
BpiI GAAGAC 1 cut(s) 1061
BpmI CTGGAG 2 cut(s) 665, 1472
Bpu10I CCTNAGC 2 cut(s) 321, 1086
BpuEI CTTGAG 2 cut(s) 569, 755
BpuMI CCSGG 1 cut(s) 1603
BsaBI GATNNNNATC 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 1289
BsaI GGTCTC 1 cut(s) 1324
BsaJI CCNNGG 2 cut(s) 508, 1121
BsaWI WCCGGW 1 cut(s) 1712
Bsc4I CCNNNNNNNGG 6 cut(s) 229, 1518, 1519, 1659, 1686, 1687
Bse1I ACTGG 3 cut(s) 815, 886, 932
Bse21I CCTNAGG 1 cut(s) 1335
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 1 cut(s) 1122
BseDI CCNNGG 2 cut(s) 508, 1121
BseGI GGATG 4 cut(s) 249, 293, 714, 828
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 6 cut(s) 229, 1518, 1519, 1659, 1686, 1687
BseNI ACTGG 3 cut(s) 815, 886, 932
BseRI GAGGAG 3 cut(s) 201, 204, 234
BseSI GKGCMC 2 cut(s) 484, 1462
BseXI GCAGC 8 cut(s) 29, 143, 220, 277, 280, 567, 1396, 1520
Bsh1236I CGCG 1 cut(s) 520
BshFI GGCC 4 cut(s) 75, 507, 622, 1652
BshNI GGYRCC 1 cut(s) 1004
BsiHKAI GWGCWC 3 cut(s) 101, 484, 1462
BsiSI CCGG 2 cut(s) 1602, 1713
BsiWI CGTACG 1 cut(s) 515
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 6 cut(s) 229, 1518, 1519, 1659, 1686, 1687
BsmAI GTCTC 3 cut(s) 735, 1147, 1324
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 4 cut(s) 75, 507, 622, 1652
Bso31I GGTCTC 1 cut(s) 1324
Bsp1286I GDGCHC 3 cut(s) 101, 484, 1462
Bsp143I GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 4 cut(s) 437, 1271, 1387, 1752
BspANI GGCC 4 cut(s) 75, 507, 622, 1652
BspFNI CGCG 1 cut(s) 520
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 3 cut(s) 306, 621, 1006
BspMI ACCTGC 3 cut(s) 731, 1783, 1828
BspPI GGATC 2 cut(s) 232, 959
BspT107I GGYRCC 1 cut(s) 1004
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 1324
BsrI ACTGG 3 cut(s) 815, 886, 932
BssECI CCNNGG 2 cut(s) 508, 1121
BssMI GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
BssNI GRCGYC 1 cut(s) 1289
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 1 cut(s) 1122
Bst4CI ACNGT 7 cut(s) 107, 538, 879, 1105, 1252, 1363, 1532
Bst6I CTCTTC 4 cut(s) 430, 661, 1215, 1802
BstACI GRCGYC 1 cut(s) 1289
BstAFI CTTAAG 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 560, 591, 981, 1275, 1608, 1749
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 4 cut(s) 249, 293, 714, 828
BstFNI CGCG 1 cut(s) 520
BstHHI GCGC 3 cut(s) 522, 1279, 1825
BstKTI GATC 6 cut(s) 28, 240, 799, 961, 967, 1381
BstMAI GTCTC 3 cut(s) 735, 1147, 1324
BstMBI GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
BstMWI GCNNNNNNNGC 9 cut(s) 54, 564, 719, 746, 1274, 1472, 1514, 1715, 1760
BstNI CCWGG 1 cut(s) 1122
BstNSI RCATGY 3 cut(s) 562, 983, 1160
BstSCI CCNGG 2 cut(s) 1120, 1601
BstSLI GKGCMC 2 cut(s) 484, 1462
BstUI CGCG 1 cut(s) 520
BstV1I GCAGC 8 cut(s) 29, 143, 220, 277, 280, 567, 1396, 1520
BstV2I GAAGAC 1 cut(s) 1061
BstXI CCANNNNNNTGG 1 cut(s) 944
Bsu36I CCTNAGG 1 cut(s) 1335
BsuRI GGCC 4 cut(s) 75, 507, 622, 1652
BtgI CCRYGG 1 cut(s) 508
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 249, 293, 714, 828
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 103, 1081
BveI ACCTGC 3 cut(s) 731, 1783, 1828
Cac8I GCNNGC 6 cut(s) 560, 591, 981, 1275, 1608, 1749
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 961
CfoI GCGC 3 cut(s) 522, 1279, 1825
Cfr13I GGNCC 3 cut(s) 620, 787, 1650
Csp6I GTAC 3 cut(s) 289, 313, 516
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 8 cut(s) 143, 298, 509, 559, 962, 980, 1157, 1199
CviQI GTAC 3 cut(s) 289, 313, 516
DpnI GATC 6 cut(s) 27, 239, 798, 960, 966, 1380
DpnII GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
Eam1104I CTCTTC 4 cut(s) 430, 661, 1215, 1802
EarI CTCTTC 4 cut(s) 430, 661, 1215, 1802
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 1324
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 4 cut(s) 522, 1239, 1428, 1719
Eco81I CCTNAGG 1 cut(s) 1335
EcoO109I RGGNCCY 2 cut(s) 620, 787
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 1120
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 1 cut(s) 981
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 8 cut(s) 146, 301, 512, 562, 965, 983, 1160, 1202
FaqI GGGAC 1 cut(s) 371
FatI CATG 8 cut(s) 142, 297, 508, 558, 961, 979, 1156, 1198
FbaI TGATCA 1 cut(s) 25
FblI GTMKAC 1 cut(s) 1242
FokI GGATG 4 cut(s) 236, 280, 701, 815
FspBI CTAG 3 cut(s) 663, 909, 1659
GlaI GCGC 3 cut(s) 521, 1278, 1824
GsuI CTGGAG 2 cut(s) 665, 1472
HaeIII GGCC 4 cut(s) 75, 507, 622, 1652
HapII CCGG 2 cut(s) 1602, 1713
HhaI GCGC 3 cut(s) 522, 1279, 1825
Hin1I GRCGYC 1 cut(s) 1289
Hin1II CATG 8 cut(s) 146, 301, 512, 562, 965, 983, 1160, 1202
Hin6I GCGC 3 cut(s) 520, 1277, 1823
HinP1I GCGC 3 cut(s) 520, 1277, 1823
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HpaII CCGG 2 cut(s) 1602, 1713
HphI GGTGA 5 cut(s) 360, 1110, 1138, 1322, 1751
Hpy166II GTNNAC 6 cut(s) 313, 363, 482, 937, 1243, 1460
Hpy8I GTNNAC 6 cut(s) 313, 363, 482, 937, 1243, 1460
HpyAV CCTTC 7 cut(s) 343, 497, 590, 633, 758, 795, 1691
HpyCH4III ACNGT 7 cut(s) 107, 538, 879, 1105, 1252, 1363, 1532
HpyCH4IV ACGT 2 cut(s) 546, 1289
HpyCH4V TGCA 9 cut(s) 48, 301, 482, 562, 869, 979, 1306, 1460, 1645
HpyF10VI GCNNNNNNNGC 9 cut(s) 54, 564, 719, 746, 1274, 1472, 1514, 1715, 1760
HpySE526I ACGT 2 cut(s) 546, 1289
Hsp92I GRCGYC 1 cut(s) 1289
Hsp92II CATG 8 cut(s) 146, 301, 512, 562, 965, 983, 1160, 1202
HspAI GCGC 3 cut(s) 520, 1277, 1823
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
LmnI GCTCC 1 cut(s) 1273
Lsp1109I GCAGC 8 cut(s) 29, 143, 220, 277, 280, 567, 1396, 1520
LweI GCATC 3 cut(s) 29, 834, 1124
MaeI CTAG 3 cut(s) 663, 909, 1659
MaeII ACGT 2 cut(s) 546, 1289
MaeIII GTNAC 3 cut(s) 532, 595, 604
MalI GATC 6 cut(s) 27, 239, 798, 960, 966, 1380
MboI GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
MboII GAAGA 8 cut(s) 76, 417, 678, 806, 1061, 1141, 1232, 1819
MfeI CAATTG 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 101, 484, 1462
MluCI AATT 6 cut(s) 4, 34, 454, 1232, 1488, 1534
MlyI GAGTC 7 cut(s) 403, 428, 678, 737, 762, 1037, 1620
MmeI TCCRAC 1 cut(s) 1183
Mph1103I ATGCAT 1 cut(s) 981
MseI TTAA 2 cut(s) 405, 492
MslI CAYNNNNRTG 1 cut(s) 1161
MspA1I CMGCKG 1 cut(s) 1387
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 2 cut(s) 1602, 1713
MspR9I CCNGG 2 cut(s) 1122, 1603
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 1122
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 9 cut(s) 54, 564, 719, 746, 1274, 1472, 1514, 1715, 1760
NciI CCSGG 1 cut(s) 1603
NcoI CCATGG 1 cut(s) 508
NdeII GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
NlaIII CATG 8 cut(s) 146, 301, 512, 562, 965, 983, 1160, 1202
NlaIV GGNNCC 3 cut(s) 306, 621, 1006
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 2 cut(s) 595, 604
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 3 cut(s) 562, 983, 1160
PaeI GCATGC 2 cut(s) 562, 983
PagI TCATGA 1 cut(s) 961
PaqCI CACCTGC 2 cut(s) 731, 1783
PciI ACATGT 1 cut(s) 1156
PcsI WCGNNNNNNNCGW 1 cut(s) 1603
PfeI GAWTC 8 cut(s) 443, 497, 896, 901, 1022, 1259, 1742, 1813
Pfl23II CGTACG 1 cut(s) 515
PflFI GACNNNGTC 1 cut(s) 1832
PleI GAGTC 7 cut(s) 403, 427, 677, 736, 762, 1037, 1620
PpsI GAGTC 7 cut(s) 403, 427, 677, 736, 762, 1037, 1620
PpuMI RGGWCCY 1 cut(s) 787
PscI ACATGT 1 cut(s) 1156
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 1 cut(s) 1120
PspGI CCWGG 1 cut(s) 1120
PspLI CGTACG 1 cut(s) 515
PspN4I GGNNCC 3 cut(s) 306, 621, 1006
PspPI GGNCC 3 cut(s) 620, 787, 1650
PspPPI RGGWCCY 1 cut(s) 787
PstNI CAGNNNCTG 1 cut(s) 23
PsyI GACNNNGTC 1 cut(s) 1832
RsaI GTAC 3 cut(s) 290, 314, 517
RsaNI GTAC 3 cut(s) 289, 313, 516
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 2 cut(s) 405, 492
Sau3AI GATC 6 cut(s) 25, 237, 796, 958, 964, 1378
Sau96I GGNCC 3 cut(s) 620, 787, 1650
SchI GAGTC 7 cut(s) 403, 428, 678, 737, 762, 1037, 1620
ScrFI CCNGG 2 cut(s) 1122, 1603
SduI GDGCHC 3 cut(s) 101, 484, 1462
SfaNI GCATC 3 cut(s) 29, 834, 1124
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 3 cut(s) 404, 584, 770
SmoI CTYRAG 3 cut(s) 404, 584, 770
SphI GCATGC 2 cut(s) 562, 983
Sse9I AATT 6 cut(s) 4, 34, 454, 1232, 1488, 1534
SsiI CCGC 4 cut(s) 437, 1271, 1387, 1752
SspI AATATT 1 cut(s) 1398
SspMI CTAG 3 cut(s) 663, 909, 1659
StyD4I CCNGG 2 cut(s) 1120, 1601
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 7 cut(s) 107, 538, 879, 1105, 1252, 1363, 1532
TaiI ACGT 2 cut(s) 549, 1292
TaqI TCGA 2 cut(s) 799, 899
TasI AATT 6 cut(s) 4, 34, 454, 1232, 1488, 1534
TauI GCSGC 2 cut(s) 440, 1754
TfiI GAWTC 8 cut(s) 443, 497, 896, 901, 1022, 1259, 1742, 1813
Tru1I TTAA 2 cut(s) 405, 492
Tru9I TTAA 2 cut(s) 405, 492
TscAI CASTG 3 cut(s) 94, 110, 1081
TseFI GTSAC 2 cut(s) 595, 604
TseI GCWGC 8 cut(s) 17, 156, 233, 265, 268, 555, 1384, 1508
Tsp45I GTSAC 2 cut(s) 595, 604
TspDTI ATGAA 6 cut(s) 17, 360, 1006, 1187, 1598, 1710
TspGWI ACGGA 2 cut(s) 682, 1495
TspRI CASTG 3 cut(s) 94, 110, 1081
Tth111I GACNNNGTC 1 cut(s) 1832
Vha464I CTTAAG 1 cut(s) 404
VneI GTGCAC 2 cut(s) 480, 1458
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 2 cut(s) 4, 454
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 3 cut(s) 562, 983, 1160
XmiI GTMKAC 1 cut(s) 1242
XspI CTAG 3 cut(s) 663, 909, 1659
ZraI GACGTC 1 cut(s) 1290
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.