RLG00000032420

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000360
Physical Location & Seq
Forward (+)
15019940 .. 15028546
8607 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032420

Sequence Viewer

Length: 576 bp
ATGAGTTCCCATGCACGGCAGAGGGCCTCGGCTAGGGGTAACGCAGCAGGCTCGTGCCTGGCCGACAAGGCAGATCGCAAACAACTTGCTACTAAAGCAGCGGGTCGTGCTACTTTTGAAACACCCGCTATGGTTAGAATGAAAAATTGCGCAATCTGCTGGAGTAACGTGACAACAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTCTTTCCACTACCCTTGCATTGCCAACAATGTCGATCATGGAAATCTTTGCTGCCCCATCTGCCGAGCAAAATGGGATAAGAATAATGTTCCTTTCCAAGTTCCCCCGCTTCAACAAAACAACTTGGGTGCACATGGCTTCGCTGATGATGAACCTCTCCCGTTCACCTCACCTGCTCAATCTTCTGGCCCCCAGAATGTCACAATCAAAACTCATACAGAGACCTCTGCTATCCCTGCTGCCGATTCACGCCCACAATATCCTGTTCTAGTCAGCATCTGTGCACCACCCCTTCAGGATCCTGATGGCGAAGTCCGTACACCGATTGACCTTGTAACAGTTCTAGACGTAACCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

192

Amino Acids

20.44

Weight (kDa)

6.57

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_11 PF17123 49 - 79 1.2e-10 RING-like zinc finger
zf-RING_2 PF13639 49 - 94 5e-07 Ring finger domain
zf-C3HC4 PF00097 50 - 93 4.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 397
Acc16I TGCGCA 1 cut(s) 151
Acc36I ACCTGC 1 cut(s) 397
AciI CCGC 3 cut(s) 101, 126, 323
AclWI GGATC 2 cut(s) 509, 522
AcoI YGGCCR 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 494
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 2 cut(s) 15, 33
AgsI TTSAA 2 cut(s) 119, 329
AjnI CCWGG 1 cut(s) 57
Alw21I GWGCWC 3 cut(s) 212, 349, 502
Alw26I GTCTC 1 cut(s) 431
Alw44I GTGCAC 2 cut(s) 345, 498
AlwI GGATC 2 cut(s) 509, 522
AlwNI CAGNNNCTG 1 cut(s) 495
AoxI GGCC 4 cut(s) 24, 60, 184, 403
ApaLI GTGCAC 2 cut(s) 345, 498
ApeKI GCWGC 4 cut(s) 44, 98, 267, 455
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 24, 404
AsuHPI GGTGA 2 cut(s) 373, 378
BaeGI GKGCMC 2 cut(s) 349, 502
BamHI GGATCC 1 cut(s) 514
BauI CACGAG 1 cut(s) 52
Bbv12I GWGCWC 3 cut(s) 212, 349, 502
BbvI GCAGC 4 cut(s) 56, 110, 254, 442
BccI CCATC 3 cut(s) 200, 281, 515
BceAI ACGGC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 3 cut(s) 33, 485, 560
BfuAI ACCTGC 1 cut(s) 397
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 4 cut(s) 45, 99, 268, 456
BlsI GCNGC 4 cut(s) 46, 100, 269, 457
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 24, 404
BmiI GGNNCC 2 cut(s) 406, 516
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 181
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 33
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 27
BseLI CCNNNNNNNGG 2 cut(s) 15, 33
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 349, 502
BseXI GCAGC 4 cut(s) 56, 110, 254, 442
BshFI GGCC 4 cut(s) 26, 62, 186, 405
BsiHKAI GWGCWC 3 cut(s) 212, 349, 502
BslI CCNNNNNNNGG 2 cut(s) 15, 33
BsmAI GTCTC 1 cut(s) 431
BsnI GGCC 4 cut(s) 26, 62, 186, 405
Bso31I GGTCTC 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 212, 349, 502
Bsp143I GATC 3 cut(s) 73, 250, 514
BspACI CCGC 3 cut(s) 101, 126, 323
BspANI GGCC 4 cut(s) 26, 62, 186, 405
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 406, 516
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 2 cut(s) 509, 522
BspTNI GGTCTC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 73, 250, 514
BssSI CACGAG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 31
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 76, 253, 517
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 3 cut(s) 73, 250, 514
BstMWI GCNNNNNNNGC 6 cut(s) 68, 95, 107, 156, 276, 452
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSLI GKGCMC 2 cut(s) 349, 502
BstV1I GCAGC 4 cut(s) 56, 110, 254, 442
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 4 cut(s) 26, 62, 186, 405
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 49
CaiI CAGNNNCTG 1 cut(s) 495
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 24, 404
Csp6I GTAC 1 cut(s) 534
CspCI CAANNNNNGTGG 2 cut(s) 212, 247
CviAII CATG 3 cut(s) 11, 254, 350
CviJI RGCY 8 cut(s) 26, 32, 51, 62, 186, 191, 354, 405
CviKI_1 RGCY 8 cut(s) 26, 32, 51, 62, 186, 191, 354, 405
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 1 cut(s) 204
DpnI GATC 3 cut(s) 75, 252, 516
DpnII GATC 3 cut(s) 73, 250, 514
EaeI YGGCCR 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 14, 257, 353
FaiI YATR 5 cut(s) 12, 131, 255, 351, 432
FatI CATG 3 cut(s) 10, 253, 349
FauI CCCGC 3 cut(s) 94, 133, 330
Fnu4HI GCNGC 4 cut(s) 45, 99, 268, 456
Fsp4HI GCNGC 4 cut(s) 45, 99, 268, 456
FspBI CTAG 3 cut(s) 33, 485, 560
FspI TGCGCA 1 cut(s) 151
GlaI GCGC 1 cut(s) 151
GluI GCNGC 4 cut(s) 45, 99, 268, 456
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 4 cut(s) 26, 62, 186, 405
HhaI GCGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 14, 257, 353
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 1 cut(s) 461
HphI GGTGA 2 cut(s) 373, 378
Hpy166II GTNNAC 4 cut(s) 347, 381, 500, 536
Hpy188III TCNNGA 3 cut(s) 512, 518, 560
Hpy8I GTNNAC 4 cut(s) 347, 381, 500, 536
HpyAV CCTTC 1 cut(s) 518
HpyCH4III ACNGT 1 cut(s) 556
HpyCH4IV ACGT 2 cut(s) 168, 564
HpyCH4V TGCA 4 cut(s) 14, 234, 347, 500
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 95, 107, 156, 276, 452
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 2 cut(s) 168, 564
Hsp92II CATG 3 cut(s) 14, 257, 353
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 73, 250, 514
Lsp1109I GCAGC 4 cut(s) 56, 110, 254, 442
LweI GCATC 1 cut(s) 501
MaeI CTAG 3 cut(s) 33, 485, 560
MaeII ACGT 2 cut(s) 168, 564
MaeIII GTNAC 6 cut(s) 38, 164, 169, 415, 550, 565
MalI GATC 3 cut(s) 75, 252, 516
MboI GATC 3 cut(s) 73, 250, 514
MboII GAAGA 2 cut(s) 187, 390
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 3 cut(s) 212, 349, 502
MluCI AATT 1 cut(s) 145
MnlI CCTC 5 cut(s) 15, 37, 381, 394, 451
MspA1I CMGCKG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 6 cut(s) 68, 95, 107, 156, 276, 452
NdeII GATC 3 cut(s) 73, 250, 514
NlaIII CATG 3 cut(s) 14, 257, 353
NlaIV GGNNCC 2 cut(s) 406, 516
NmeAIII GCCGAG 2 cut(s) 8, 305
NmuCI GTSAC 2 cut(s) 169, 415
NsbI TGCGCA 1 cut(s) 151
PaqCI CACCTGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 461
PkrI GCNGC 4 cut(s) 46, 100, 269, 457
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 406, 516
PspPI GGNCC 2 cut(s) 24, 404
PstNI CAGNNNCTG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
SatI GCNGC 4 cut(s) 45, 99, 268, 456
Sau3AI GATC 3 cut(s) 73, 250, 514
Sau96I GGNCC 2 cut(s) 24, 404
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 3 cut(s) 212, 349, 502
SetI ASST 7 cut(s) 171, 373, 386, 391, 443, 549, 567
SfaNI GCATC 1 cut(s) 501
Sse9I AATT 1 cut(s) 145
SsiI CCGC 3 cut(s) 101, 126, 323
SspMI CTAG 3 cut(s) 33, 485, 560
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 556
TaiI ACGT 2 cut(s) 171, 567
TaqI TCGA 1 cut(s) 249
TasI AATT 1 cut(s) 145
TfiI GAWTC 1 cut(s) 461
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 2 cut(s) 169, 415
TseI GCWGC 4 cut(s) 44, 98, 267, 455
Tsp45I GTSAC 2 cut(s) 169, 415
TspDTI ATGAA 2 cut(s) 155, 381
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 205
VneI GTGCAC 2 cut(s) 345, 498
XagI CCTNNNNNAGG 1 cut(s) 31
XbaI TCTAGA 1 cut(s) 559
XspI CTAG 3 cut(s) 33, 485, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.