Rh5AG139000

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000360
Physical Location & Seq
Forward (+)
14436574 .. 14438948
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG139000.1

Sequence Viewer

Length: 1764 bp
ATGTCGCGACAGTATAGTTTACGGTCTCAGGGTCCATCTTCTCATAACATTTGCTCCATCTGCTGGAGTAACTTGACAAACGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTCTTTCCACCACCATTGCATTGCCAACAATGTCCAGCATGGAAATCTTTGCTGCCCTGCCTGCAGAGCAAAATGGAATAAAAATAATTTACCTTTCCAAGTTCCCCCTCTTCAACAAAATAACAAGAGTAAACATGGCTTCGCCGATGATGAACCTCTCTCGTCCGCCACACTCCTCAAAACTTCTGGCCCCCAGAATGTAACAGTCAAGACTCATACAGAGACCTCTGCTATCTCTGCTGCCGAATCACGCTCCCATTATCCTGTTCTAGTTAGCATTTGTGCACCACCCCTTCAAGATCCCGATGGTGAAGGCCGTACACCCATTGACCTTGTTACAATTCTGGACGTAAGCGGTAGCATGCATGGCCAAAAGCTTGAACTTGTCAAGCAAGCTGTCAAATTCGTCATTGAAAACATGGGGACTTCGGACAGACTGTCTATAGTATCATTCTCAACAACTTCTAAACGACTCCTTCCTCTCCGTAGAATGACTGTTAAGGGCCGTGCAAGTGCAATCCAAGCTGTCGAATCTCTTAGAGCAGATGGTGGAACTGACATTGCAGAAGGACTCAAGATAGGAACTCATATCCTTGAGGAGCGAAGGGAAAGGAACCCTGTTGCTAGCATCATCCTCTTATCGGATGGCCAAGATAGCTACTGTGACAACCCAAGCCAAATGTTGAAGAAATTGCCTGCTTCAATTCGTTCTAGTGACATGCAACATGAAATCCCAGTCCACACATTTGGGTTTGGCAACGACCATAATGCAAAGATTATGCATACTATATCTAATGTATCAGGGGGAACGTTTTCGTACATTGAATCAGTTGGCATAATACAAGACGCTTTTGCTCTGTGCATTGGTGGTCTTCTCAGTGTTGTGGTTCAGGAACTTCGCCTCACGGTGAGGTCAGCATCACCTGGGGTGAAGATCGTGGAAATACCATCAGGGAGACATGTCAGTCAGATTTCTGATGAGCAAGGTGTTGTTGATGTTGGAAATATGTATGCAGAAGAGGAGAAACAGTTTCTGGTGTACCTCTCAGTTCCACAATCCGCAGCTCCGGATACCAAGACGTCTTTGGTAGACGTTTTATGCGTGTACAAAGATCTTGGTTCAGATGAGTTTATCCCTGTCCAAGGTGAGAAAGTAGAGATAACGAGACCTGATTTTTGTTCTCCTGCAGAAATGGCAGTGTCTTTGGAGGTTGATCGGCAGCGGAACAGACTTTTGGTGGCTGATGCTATTGGAGAGGCACAAAGGTTGGCTGAAATGGGCAATCTGGAGGCTGCACGGGCGGTTTTGACTCAGCGAAGAGAAACTCTCATGACATCAACAGCAGCCAAAGCTGGGGATGATCTGAGTCATATGTTTGAAACTGAGCTTAAACAAATCATGGATAAAATGGCAAGTATGAATTTGTATGAAGAGGTGGGGCGTGCTTACGCTCTCTCAGGGATGAGTTCCCATGCACGGCAGAGGGCCTCGGCTAGGGGTAACGCAGCAGGCTCGTGCCTGGCCGACAAGGCAGGTCGCAAACAACTTGCTACTAAAGCAGCGGGTCGTGCTACTTTTGAAACACCCGCTATGGTTAGAATGGTAAGAAAATCACAGAATAAGAGGAAGTCTGGTTCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

587

Amino Acids

63.77

Weight (kDa)

8.16

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 17 - 62 2.8e-06 Ring finger domain
zf-RING_11 PF17123 18 - 47 3.1e-08 RING-like zinc finger
VWA_CoxE PF05762 146 - 264 3e-06 VWA domain containing CoxE-like protein
VWA PF00092 150 - 316 6.6e-23 von Willebrand factor type A domain
VWA_3 PF13768 150 - 311 4.6e-19 von Willebrand factor type A domain
VWA_2 PF13519 151 - 257 1.3e-18 von Willebrand factor type A domain
Vwaint PF14624 499 - 579 1e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1083
AatII GACGTC 1 cut(s) 1202
Acc36I ACCTGC 1 cut(s) 1643
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 1 cut(s) 1209
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 1186
AciI CCGC 7 cut(s) 285, 474, 1179, 1342, 1421, 1682, 1707
AclI AACGTT 1 cut(s) 929
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 3 cut(s) 487, 766, 1641
AcsI RAATTY 2 cut(s) 521, 1540
AcyI GRCGYC 1 cut(s) 1199
AfaI GTAC 4 cut(s) 439, 938, 1160, 1226
AfiI CCNNNNNNNGG 6 cut(s) 63, 760, 1262, 1473, 1596, 1614
AflIII ACRYGT 1 cut(s) 1078
AgsI TTSAA 9 cut(s) 233, 416, 500, 533, 805, 822, 944, 1499, 1700
AhdI GACNNNNNGTC 1 cut(s) 556
AjnI CCWGG 2 cut(s) 1042, 1638
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AloI GAACNNNNNNTCC 1 cut(s) 1738
AluBI AGCT 7 cut(s) 496, 515, 644, 777, 1184, 1472, 1507
AluI AGCT 7 cut(s) 496, 515, 644, 777, 1184, 1472, 1507
Alw21I GWGCWC 2 cut(s) 116, 406
Alw26I GTCTC 4 cut(s) 30, 335, 1069, 1279
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 1 cut(s) 413
AlwNI CAGNNNCTG 1 cut(s) 1153
Aor13HI TCCGGA 1 cut(s) 1186
AoxI GGCC 8 cut(s) 88, 307, 433, 487, 622, 766, 1605, 1641
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 8 cut(s) 171, 359, 1181, 1339, 1412, 1463, 1625, 1679
ApoI RAATTY 2 cut(s) 521, 1540
ArsI GACNNNNNNTTYG 2 cut(s) 1278, 1310
Asp700I GAANNNNTTC 1 cut(s) 931
AspS9I GGNCC 4 cut(s) 32, 308, 622, 1605
AsuHPI GGTGA 5 cut(s) 440, 1032, 1039, 1060, 1277
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 406
BalI TGGCCA 2 cut(s) 489, 768
BauI CACGAG 1 cut(s) 1633
BbsI GAAGAC 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 116, 406
BbvI GCAGC 8 cut(s) 158, 346, 1193, 1351, 1399, 1475, 1637, 1691
BccI CCATC 7 cut(s) 43, 65, 104, 419, 659, 758, 1075
BceAI ACGGC 3 cut(s) 420, 609, 1613
BcgI CGANNNNNNTGC 4 cut(s) 869, 903, 1614, 1648
BciT130I CCWGG 2 cut(s) 1044, 1640
BciVI GTATCC 1 cut(s) 1183
BcoDI GTCTC 4 cut(s) 30, 335, 1069, 1279
BfaI CTAG 4 cut(s) 389, 744, 831, 1614
BfmI CTRYAG 3 cut(s) 181, 561, 1305
BfuAI ACCTGC 1 cut(s) 1643
BfuI GTATCC 1 cut(s) 1183
BglI GCCNNNNNGGC 1 cut(s) 1649
BglII AGATCT 1 cut(s) 1231
BisI GCNGC 8 cut(s) 172, 360, 1182, 1340, 1413, 1464, 1626, 1680
BlsI GCNGC 8 cut(s) 173, 361, 1183, 1341, 1414, 1465, 1627, 1681
Bme1390I CCNGG 2 cut(s) 1044, 1640
Bme18I GGWCC 1 cut(s) 32
BmeRI GACNNNNNGTC 1 cut(s) 556
BmgT120I GGNCC 4 cut(s) 32, 308, 622, 1605
BmiI GGNNCC 3 cut(s) 33, 310, 734
BmrFI CCNGG 2 cut(s) 1044, 1640
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 3 cut(s) 756, 1046, 1354
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 848
BpiI GAAGAC 1 cut(s) 983
BplI GAGNNNNNCTC 2 cut(s) 1431, 1463
BpmI CTGGAG 2 cut(s) 85, 1427
BpuEI CTTGAG 2 cut(s) 677, 734
BsaHI GRCGYC 1 cut(s) 1199
BsaI GGTCTC 3 cut(s) 30, 335, 1279
BsaJI CCNNGG 3 cut(s) 1043, 1261, 1608
BsaWI WCCGGW 1 cut(s) 1186
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 1133, 1163
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 760, 1262, 1473, 1596, 1614
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 3 cut(s) 133, 138, 678
BseAI TCCGGA 1 cut(s) 1186
BseBI CCWGG 2 cut(s) 1044, 1640
BseDI CCNNGG 3 cut(s) 1043, 1261, 1608
BseGI GGATG 4 cut(s) 750, 769, 1483, 1587
BseLI CCNNNNNNNGG 6 cut(s) 63, 760, 1262, 1473, 1596, 1614
BseMI GCAATG 3 cut(s) 133, 138, 678
BseMII CTCAG 8 cut(s) 41, 99, 1009, 1179, 1445, 1475, 1494, 1590
BseNI ACTGG 1 cut(s) 854
BseRI GAGGAG 3 cut(s) 284, 731, 1154
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 8 cut(s) 158, 346, 1193, 1351, 1399, 1475, 1637, 1691
BseYI CCCAGC 1 cut(s) 1472
BsgI GTGCAG 1 cut(s) 1398
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 8 cut(s) 90, 309, 435, 489, 624, 768, 1607, 1643
BsiHKAI GWGCWC 2 cut(s) 116, 406
BsiSI CCGG 1 cut(s) 1187
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 6 cut(s) 63, 760, 1262, 1473, 1596, 1614
BsmAI GTCTC 4 cut(s) 30, 335, 1069, 1279
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 8 cut(s) 90, 309, 435, 489, 624, 768, 1607, 1643
Bso31I GGTCTC 3 cut(s) 30, 335, 1279
Bsp1286I GDGCHC 2 cut(s) 116, 406
Bsp13I TCCGGA 1 cut(s) 1186
Bsp1407I TGTACA 1 cut(s) 1224
Bsp143I GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
Bsp68I TCGCGA 1 cut(s) 7
BspACI CCGC 7 cut(s) 285, 474, 1179, 1342, 1421, 1682, 1707
BspANI GGCC 8 cut(s) 90, 309, 435, 489, 624, 768, 1607, 1643
BspCNI CTCAG 8 cut(s) 40, 100, 1008, 1178, 1444, 1476, 1495, 1589
BspEI TCCGGA 1 cut(s) 1186
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 1449
BspLI GGNNCC 3 cut(s) 33, 310, 734
BspMAI CTGCAG 2 cut(s) 185, 1309
BspMI ACCTGC 1 cut(s) 1643
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 1 cut(s) 413
BspTNI GGTCTC 3 cut(s) 30, 335, 1279
BsrDI GCAATG 3 cut(s) 133, 138, 678
BsrGI TGTACA 1 cut(s) 1224
BsrI ACTGG 1 cut(s) 854
BssECI CCNNGG 3 cut(s) 1043, 1261, 1608
BssMI GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
BssNI GRCGYC 1 cut(s) 1199
BssSI CACGAG 1 cut(s) 1633
BssT1I CCWWGG 1 cut(s) 1261
Bst2BI CACGAG 1 cut(s) 1633
Bst2UI CCWGG 2 cut(s) 1044, 1640
Bst4CI ACNGT 8 cut(s) 12, 24, 325, 558, 616, 782, 1027, 1149
Bst6I CTCTTC 4 cut(s) 234, 1131, 1432, 1545
BstACI GRCGYC 1 cut(s) 1199
BstAUI TGTACA 1 cut(s) 1224
BstC8I GCNNGC 7 cut(s) 181, 482, 513, 745, 816, 1563, 1630
BstDEI CTNAG 9 cut(s) 27, 108, 656, 995, 1165, 1431, 1484, 1503, 1576
BstENI CCTNNNNNAGG 2 cut(s) 1260, 1612
BstF5I GGATG 4 cut(s) 750, 769, 1483, 1587
BstFNI CGCG 1 cut(s) 7
BstKTI GATC 5 cut(s) 421, 1056, 1234, 1336, 1483
BstMAI GTCTC 4 cut(s) 30, 335, 1069, 1279
BstMBI GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
BstNI CCWGG 2 cut(s) 1044, 1640
BstNSI RCATGY 3 cut(s) 484, 841, 1082
BstSCI CCNGG 2 cut(s) 1042, 1638
BstSFI CTRYAG 3 cut(s) 181, 561, 1305
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 8 cut(s) 158, 346, 1193, 1351, 1399, 1475, 1637, 1691
BstV2I GAAGAC 1 cut(s) 983
BstX2I RGATCY 2 cut(s) 418, 1231
BstXI CCANNNNNNTGG 1 cut(s) 866
BstYI RGATCY 2 cut(s) 418, 1231
BsuI GTATCC 1 cut(s) 1183
BsuRI GGCC 8 cut(s) 90, 309, 435, 489, 624, 768, 1607, 1643
BtsCI GGATG 4 cut(s) 750, 769, 1483, 1587
BtsI GCAGTG 2 cut(s) 102, 1323
BtsIMutI CAGTG 3 cut(s) 102, 1003, 1323
BtuMI TCGCGA 1 cut(s) 7
BveI ACCTGC 1 cut(s) 1643
Cac8I GCNNGC 7 cut(s) 181, 482, 513, 745, 816, 1563, 1630
CaiI CAGNNNCTG 1 cut(s) 1153
CciI TCATGA 1 cut(s) 1449
Cfr13I GGNCC 4 cut(s) 32, 308, 622, 1605
CseI GACGC 1 cut(s) 974
Csp6I GTAC 4 cut(s) 438, 937, 1159, 1225
CspCI CAANNNNNGTGG 2 cut(s) 116, 151
CviQI GTAC 4 cut(s) 438, 937, 1159, 1225
DdeI CTNAG 9 cut(s) 27, 108, 656, 995, 1165, 1431, 1484, 1503, 1576
DpnI GATC 5 cut(s) 420, 1055, 1233, 1335, 1482
DpnII GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
DrdI GACNNNNNNGTC 1 cut(s) 1083
DriI GACNNNNNGTC 1 cut(s) 556
DseDI GACNNNNNNGTC 1 cut(s) 1083
EaeI YGGCCR 3 cut(s) 487, 766, 1641
Eam1104I CTCTTC 4 cut(s) 234, 1131, 1432, 1545
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 4 cut(s) 234, 1131, 1432, 1545
EciI GGCGGA 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 1261
Eco31I GGTCTC 3 cut(s) 30, 335, 1279
Eco47I GGWCC 1 cut(s) 32
EcoNI CCTNNNNNAGG 2 cut(s) 1260, 1612
EcoO109I RGGNCCY 1 cut(s) 1605
EcoRII CCWGG 2 cut(s) 1042, 1638
EcoT14I CCWWGG 1 cut(s) 1261
EcoT22I ATGCAT 2 cut(s) 486, 903
ErhI CCWWGG 1 cut(s) 1261
FaqI GGGAC 1 cut(s) 556
FauI CCCGC 2 cut(s) 1675, 1714
FauNDI CATATG 1 cut(s) 1491
FblI GTMKAC 1 cut(s) 1209
Fnu4HI GCNGC 8 cut(s) 172, 360, 1182, 1340, 1413, 1464, 1626, 1680
FokI GGATG 4 cut(s) 737, 776, 1490, 1594
Fsp4HI GCNGC 8 cut(s) 172, 360, 1182, 1340, 1413, 1464, 1626, 1680
FspBI CTAG 4 cut(s) 389, 744, 831, 1614
GluI GCNGC 8 cut(s) 172, 360, 1182, 1340, 1413, 1464, 1626, 1680
GsaI CCCAGC 1 cut(s) 1476
GsuI CTGGAG 2 cut(s) 85, 1427
HaeIII GGCC 8 cut(s) 90, 309, 435, 489, 624, 768, 1607, 1643
HapII CCGG 1 cut(s) 1187
HgaI GACGC 1 cut(s) 974
Hin1I GRCGYC 1 cut(s) 1199
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 8 cut(s) 331, 365, 591, 650, 690, 944, 1429, 1486
HpaII CCGG 1 cut(s) 1187
HphI GGTGA 5 cut(s) 440, 1032, 1039, 1060, 1277
Hpy166II GTNNAC 8 cut(s) 20, 251, 404, 440, 859, 1159, 1210, 1225
Hpy188I TCNGA 6 cut(s) 550, 763, 1089, 1096, 1243, 1485
Hpy8I GTNNAC 8 cut(s) 20, 251, 404, 440, 859, 1159, 1210, 1225
HpyAV CCTTC 5 cut(s) 422, 425, 605, 680, 717
HpyCH4III ACNGT 8 cut(s) 12, 24, 325, 558, 616, 782, 1027, 1149
HpyCH4IV ACGT 4 cut(s) 468, 929, 1199, 1212
HpyF3I CTNAG 9 cut(s) 27, 108, 656, 995, 1165, 1431, 1484, 1503, 1576
HpySE526I ACGT 4 cut(s) 468, 929, 1199, 1212
Hsp92I GRCGYC 1 cut(s) 1199
Kpn2I TCCGGA 1 cut(s) 1186
Kzo9I GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
LmnI GCTCC 4 cut(s) 59, 377, 718, 1189
Lsp1109I GCAGC 8 cut(s) 158, 346, 1193, 1351, 1399, 1475, 1637, 1691
LweI GCATC 3 cut(s) 756, 1046, 1354
MaeI CTAG 4 cut(s) 389, 744, 831, 1614
MaeII ACGT 4 cut(s) 468, 929, 1199, 1212
MaeIII GTNAC 6 cut(s) 68, 319, 454, 782, 833, 1619
MalI GATC 5 cut(s) 420, 1055, 1233, 1335, 1482
MboI GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
MboII GAAGA 9 cut(s) 30, 91, 221, 817, 983, 1063, 1148, 1449, 1562
MflI RGATCY 2 cut(s) 418, 1231
MhlI GDGCHC 2 cut(s) 116, 406
MlsI TGGCCA 2 cut(s) 489, 768
MluCI AATT 6 cut(s) 205, 459, 521, 809, 822, 1540
MluNI TGGCCA 2 cut(s) 489, 768
MlyI GAGTC 5 cut(s) 325, 585, 684, 1423, 1495
MmeI TCCRAC 1 cut(s) 1099
Mox20I TGGCCA 2 cut(s) 489, 768
Mph1103I ATGCAT 2 cut(s) 486, 903
MroI TCCGGA 1 cut(s) 1186
MroXI GAANNNNTTC 1 cut(s) 931
MscI TGGCCA 2 cut(s) 489, 768
MseI TTAA 2 cut(s) 618, 1509
Msp20I TGGCCA 2 cut(s) 489, 768
MspA1I CMGCKG 2 cut(s) 1342, 1682
MspI CCGG 1 cut(s) 1187
MspR9I CCNGG 2 cut(s) 1044, 1640
MvaI CCWGG 2 cut(s) 1044, 1640
MvnI CGCG 1 cut(s) 7
NdeI CATATG 1 cut(s) 1491
NdeII GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
NheI GCTAGC 1 cut(s) 743
NlaIV GGNNCC 3 cut(s) 33, 310, 734
NmeAIII GCCGAG 1 cut(s) 1589
NmuCI GTSAC 2 cut(s) 782, 833
NruI TCGCGA 1 cut(s) 7
NsiI ATGCAT 2 cut(s) 486, 903
NspI RCATGY 3 cut(s) 484, 841, 1082
PaeI GCATGC 1 cut(s) 484
PagI TCATGA 1 cut(s) 1449
PciI ACATGT 1 cut(s) 1078
PcsI WCGNNNNNNNCGW 1 cut(s) 1218
PdmI GAANNNNTTC 1 cut(s) 931
PfeI GAWTC 3 cut(s) 365, 650, 944
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 8 cut(s) 173, 361, 1183, 1341, 1414, 1465, 1627, 1681
PleI GAGTC 5 cut(s) 325, 585, 684, 1423, 1494
PpsI GAGTC 5 cut(s) 325, 585, 684, 1423, 1494
PscI ACATGT 1 cut(s) 1078
Psp1406I AACGTT 1 cut(s) 929
Psp6I CCWGG 2 cut(s) 1042, 1638
PspFI CCCAGC 1 cut(s) 1472
PspGI CCWGG 2 cut(s) 1042, 1638
PspN4I GGNNCC 3 cut(s) 33, 310, 734
PspPI GGNCC 4 cut(s) 32, 308, 622, 1605
PstI CTGCAG 2 cut(s) 185, 1309
PstNI CAGNNNCTG 1 cut(s) 1153
PsuI RGATCY 2 cut(s) 418, 1231
RruI TCGCGA 1 cut(s) 7
RsaI GTAC 4 cut(s) 439, 938, 1160, 1226
RsaNI GTAC 4 cut(s) 438, 937, 1159, 1225
SaqAI TTAA 2 cut(s) 618, 1509
SatI GCNGC 8 cut(s) 172, 360, 1182, 1340, 1413, 1464, 1626, 1680
Sau3AI GATC 5 cut(s) 418, 1053, 1231, 1333, 1480
Sau96I GGNCC 4 cut(s) 32, 308, 622, 1605
SchI GAGTC 5 cut(s) 325, 585, 684, 1423, 1495
ScrFI CCNGG 2 cut(s) 1044, 1640
SduI GDGCHC 2 cut(s) 116, 406
SfaNI GCATC 3 cut(s) 756, 1046, 1354
SfcI CTRYAG 3 cut(s) 181, 561, 1305
SinI GGWCC 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 692, 713
SmoI CTYRAG 2 cut(s) 692, 713
SphI GCATGC 1 cut(s) 484
Sse9I AATT 6 cut(s) 205, 459, 521, 809, 822, 1540
SsiI CCGC 7 cut(s) 285, 474, 1179, 1342, 1421, 1682, 1707
SspMI CTAG 4 cut(s) 389, 744, 831, 1614
StyD4I CCNGG 2 cut(s) 1042, 1638
StyI CCWWGG 1 cut(s) 1261
TaaI ACNGT 8 cut(s) 12, 24, 325, 558, 616, 782, 1027, 1149
TaiI ACGT 4 cut(s) 471, 932, 1202, 1215
TaqI TCGA 1 cut(s) 648
TasI AATT 6 cut(s) 205, 459, 521, 809, 822, 1540
TatI WGTACW 1 cut(s) 1224
TfiI GAWTC 3 cut(s) 365, 650, 944
Tru1I TTAA 2 cut(s) 618, 1509
Tru9I TTAA 2 cut(s) 618, 1509
TscAI CASTG 3 cut(s) 109, 1003, 1323
TseFI GTSAC 2 cut(s) 782, 833
TseI GCWGC 8 cut(s) 171, 359, 1181, 1339, 1412, 1463, 1625, 1679
Tsp45I GTSAC 2 cut(s) 782, 833
TspDTI ATGAA 4 cut(s) 285, 861, 1553, 1563
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 3 cut(s) 109, 1003, 1323
Van91I CCANNNNNTGG 1 cut(s) 63
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 32
XagI CCTNNNNNAGG 2 cut(s) 1260, 1612
XapI RAATTY 2 cut(s) 521, 1540
XceI RCATGY 3 cut(s) 484, 841, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 1201
XmiI GTMKAC 1 cut(s) 1209
XmnI GAANNNNTTC 1 cut(s) 931
XspI CTAG 4 cut(s) 389, 744, 831, 1614
ZraI GACGTC 1 cut(s) 1200
Zsp2I ATGCAT 2 cut(s) 486, 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.