FvH4_6g19090

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000241
Physical Location & Seq
Forward (+)
12639001 .. 12641593
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19090.t1

Sequence Viewer

Length: 1350 bp
ATGACAGGAGTTAAGATTTGTAGGCTAATGGCTAGTGCAAGTTGTGGTGAAGGGAGAGAAGGTTGTATTGAGAATGAGAGTAGGATAGACAGACTTAGTAGTCTTCCTGACGAGGTTGTGCATCACATTTTTTCTTTATCATGCGCAACAAAACTTGATATCCTTGATATAAAGGACCTCACTCTGTTCAGCTGTGTGTCGAAAAGATGCAGACAACTTGTGTTGTCAACTCCGACTTTGCGTTTTGATTTATTTCCGTGTCATACATCCACCTGTAAGGACCGGTTGAAGTTTATCAGTTCTTTGGATAGGCTGTTGGCTCATCGTGGCAATAATAAGTTGCAGCTCTTTATTATTTCATGGTATTCATACTGTTCAGAGGAAGGACAGTGTTTCCCAGATGATGAGAGATTTCGAGTACTGACTTGGGTAAACAATGTGCTAGTTAGGTGTAAGGTTGAAACACTATATCTTTATATGGAAGATGTCTGTATAGCTAATGATGATAACTTAGTAGCATTGCCGACTTGCATCTTTCATGCTGAATCCTTGAGGTCTCTGATGGTGGAGTTTGATTGTAAAATTCAAGTCCCCTCCTTCGCTTTTTCTTCAAATCTCAAATACCTGGAGTTGACGTCTGTTACTATAGTAGATGAGGGCTTTTTCAAGTGGATCTCATGCTGCTGCAATTGCATTGAGCAGTTATATCTTGATGATGTTTATGGGCCAAAAAGTATCACCATTCAAAGCTCATCTTTGAAATCATTTCGCTTTGAATCTTCTTATTCTAGTGAGTTGTCCCAACTTCACATCTCGGGTGAGAAGCTTAAAGAATTATCTATAGAGTGGACGTTTGATTCGCCTATGGAAATATCATTGTATATGTTTACTCCAAATCTTGAAAAGATTAACTGGTTTGGGAATTTGATGAATCACCTAAATCTGGGAAAATTTGTATGTCTGGAAGAAGCTGAAATTGGTCTGCAGCCTGCAGGGGATGACTGGAACAACGTATTTCAGGTTCTCTGCAGCTTACGTGGGGCTAAAGATGTTATTCTAACGGAACAGCTCATTAAGACATCTAGGTTTAATACAGAATTTTGGGCAGAATTTTGGGAGCAAGACCCTGCTTTTTTCAATGAGCTACAGGAGTTTACAATTGAGCTCTGGGAAGGATCTAATGGAATCGAATTAGCAAGGTATATTCTTGAGCATGCTCAAAATTTGGAGAAAATGGTCATTGTTCACTTGCCACGGCATTCCGATGTTGAAATGGTGGTCAGTAAAAGCAAGATGATTTCAAATGCCACACTGGTCTTTAAGAGTGTACCACACCACATTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

450

Amino Acids

51.61

Weight (kDa)

5.12

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 152 - 320 2.3e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000241)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18954 FvH4_6g18982 FvH4_6g19010 FvH4_6g19020 FvH4_6g19020 FvH4_6g19030 FvH4_6g19030 FvH4_6g19040 FvH4_6g19040 FvH4_6g19040 FvH4_6g19051 FvH4_6g19090 FvH4_6g19090 FvH4_6g19090 FvH4_6g33920 FvH4_6g33940
malus_domestica MD00G1221800.v1.1 MD04G1145400.v1.1 MD12G1026400.v1.1 MD12G1026500.v1.1 MD12G1026700.v1.1 MD12G1026800.v1.1 MD14G1024900.v1.1 MD14G1025000.v1.1 MD14G1025100.v1.1 MD14G1025200.v1.1 MD14G1025300.v1.1 MD14G1025400.v1.1 MD14G1025600.v1.1 MD14G1026200.v1.1 MD14G1026700.v1.1
prunus_persica Prupe.7G104800_v2.0.a1 Prupe.7G104900_v2.0.a1 Prupe.7G105000_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105600_v2.0.a1 Prupe.7G105900_v2.0.a1 Prupe.7G106200_v2.0.a1
pyrus_communis pycom04g13160 pycom07g14430 pycom12g02570 pycom14g02380 pycom14g02430 pycom14g02470 pycom14g02480 pycom14g02490
rosa_chinensis RchiOBHm_Chr3g0474001 RchiOBHm_Chr3g0474011 RchiOBHm_Chr3g0474021 RchiOBHm_Chr3g0474031 RchiOBHm_Chr3g0474041 RchiOBHm_Chr3g0474111 RchiOBHm_Chr3g0474131 RchiOBHm_Chr3g0474161 RchiOBHm_Chr3g0474191
rosa_laevigata RLG00000023848 RLG00000023942 RLG00000023944 RLG00000023946 RLG00000023948 RLG00000023956 RLG00000023957 RLG00000023958
rosa_multiflora Rmu_co8028860.1_g000001 Rmu_sc0002414.1_g000007 Rmu_sc0003170.1_g000008 Rmu_sc0003170.1_g000009 Rmu_sc0004167.1_g000001 Rmu_sc0004507.1_g000023 Rmu_sc0004507.1_g000029 Rmu_sc0004507.1_g000030 Rmu_sc0004507.1_g000032 Rmu_sc0004507.1_g000045 Rmu_sc0005137.1_g000004 Rmu_sc0015051.1_g000002 Rmu_sc0027615.1_g000001 Rmu_sc0027615.1_g000002
rosa_roxburghii Rroxscaffold_6G00407440 Rroxscaffold_6G00407450 Rroxscaffold_6G00407470 Rroxscaffold_6G00407550 Rroxscaffold_6G00407560 Rroxscaffold_6G00407570
rosa_rugosa Rorug03G0137600 Rorug03G0137600 Rorug03G0137600 Rorug03G0138800 Rorug03G0138900 Rorug03G0139200 Rorug03G0139300 Rorug03G0139300 Rorug03G0139400 Rorug03G0139500
rosa_samantha Rh3AG187700 Rh3AG187800 Rh3AG187900 Rh3AG188000 Rh3AG188100 Rh3AG188700 Rh3AG188900 Rh3AG189100 Rh3AG189200 Rh3AG189800 Rh3BG216500 Rh3BG216600 Rh3BG216700 Rh3BG216800 Rh3BG216900 Rh3BG218300 Rh3BG218500 Rh3BG219000 Rh3CG212900 Rh3CG213000 Rh3CG213100 Rh3CG213200 Rh3CG214000 Rh3CG214200 Rh3CG214400 Rh3CG222800 Rh3DG212300 Rh3DG212400 Rh3DG212500 Rh3DG212600 Rh3DG212700 Rh3DG213200 Rh3DG213500 Rh3DG213700 Rh3DG213800 Rh3DG214400 Rh3DG214500 Rh3DG223800
rosa_wichuraiana Rw3G017170 Rw3G017200 Rw3G017210 Rw3G017220 Rw3G017230 Rw3G017290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 99
AatII GACGTC 1 cut(s) 638
Acc16I TGCGCA 1 cut(s) 145
AclWI GGATC 3 cut(s) 680, 1183, 1337
AcsI RAATTY 6 cut(s) 582, 922, 950, 1097, 1109, 1222
AcyI GRCGYC 1 cut(s) 635
AfaI GTAC 2 cut(s) 420, 1329
AfiI CCNNNNNNNGG 1 cut(s) 943
AgeI ACCGGT 1 cut(s) 282
AjnI CCWGG 1 cut(s) 624
AjuI GAANNNNNNNTTGG 2 cut(s) 960, 992
Alw21I GWGCWC 1 cut(s) 1167
Alw26I GTCTC 1 cut(s) 561
AlwI GGATC 3 cut(s) 680, 1183, 1337
Ama87I CYCGRG 1 cut(s) 814
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 5 cut(s) 343, 681, 684, 985, 1029
ApoI RAATTY 6 cut(s) 582, 922, 950, 1097, 1109, 1222
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
AsiGI ACCGGT 1 cut(s) 282
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 3 cut(s) 175, 280, 725
AsuHPI GGTGA 4 cut(s) 59, 730, 830, 926
AvaI CYCGRG 1 cut(s) 814
AvaII GGWCC 2 cut(s) 175, 280
BanII GRGCYC 1 cut(s) 1167
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 1167
BbvI GCAGC 5 cut(s) 355, 668, 671, 997, 1041
BccI CCATC 1 cut(s) 556
BceAI ACGGC 1 cut(s) 1271
BciT130I CCWGG 1 cut(s) 626
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 4 cut(s) 33, 443, 789, 1083
BfmI CTRYAG 6 cut(s) 645, 840, 983, 990, 1027, 1145
BisI GCNGC 5 cut(s) 344, 682, 685, 986, 1030
BlsI GCNGC 5 cut(s) 345, 683, 686, 987, 1031
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 1 cut(s) 626
Bme18I GGWCC 2 cut(s) 175, 280
BmeT110I CYCGRG 1 cut(s) 814
BmgT120I GGNCC 3 cut(s) 175, 280, 725
BmrFI CCNGG 1 cut(s) 626
BmsI GCATC 3 cut(s) 130, 197, 540
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 571, 1229
BsaAI YACGTR 1 cut(s) 1037
BsaHI GRCGYC 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 1 cut(s) 1253
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 620, 650
Bsc4I CCNNNNNNNGG 1 cut(s) 943
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 3 cut(s) 917, 1007, 1317
Bse3DI GCAATG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 1 cut(s) 1253
BseGI GGATG 2 cut(s) 266, 1003
BseLI CCNNNNNNNGG 1 cut(s) 943
BseMI GCAATG 1 cut(s) 518
BseNI ACTGG 3 cut(s) 917, 1007, 1317
BseXI GCAGC 5 cut(s) 355, 668, 671, 997, 1041
BshFI GGCC 1 cut(s) 727
BshTI ACCGGT 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 1167
BsiHKCI CYCGRG 1 cut(s) 814
BsiSI CCGG 1 cut(s) 283
BslFI GGGAC 2 cut(s) 575, 784
BslI CCNNNNNNNGG 1 cut(s) 943
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 575, 784
BsmI GAATGC 1 cut(s) 1258
BsnI GGCC 1 cut(s) 727
Bso31I GGTCTC 1 cut(s) 561
BsoBI CYCGRG 1 cut(s) 814
Bsp1286I GDGCHC 1 cut(s) 1167
Bsp143I GATC 3 cut(s) 672, 1175, 1342
BspANI GGCC 1 cut(s) 727
BspMAI CTGCAG 3 cut(s) 987, 994, 1031
BspPI GGATC 3 cut(s) 680, 1183, 1337
BspTNI GGTCTC 1 cut(s) 561
BsrDI GCAATG 1 cut(s) 518
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 3 cut(s) 917, 1007, 1317
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 1253
BssMI GATC 3 cut(s) 672, 1175, 1342
BssNI GRCGYC 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 2 cut(s) 374, 390
BstACI GRCGYC 1 cut(s) 635
BstBAI YACGTR 1 cut(s) 1037
BstC8I GCNNGC 2 cut(s) 990, 1215
BstDEI CTNAG 2 cut(s) 95, 511
BstDSI CCRYGG 1 cut(s) 1253
BstF5I GGATG 2 cut(s) 266, 1003
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 3 cut(s) 675, 1178, 1345
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 3 cut(s) 672, 1175, 1342
BstMWI GCNNNNNNNGC 1 cut(s) 690
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 1217
BstSCI CCNGG 1 cut(s) 624
BstSFI CTRYAG 6 cut(s) 645, 840, 983, 990, 1027, 1145
BstV1I GCAGC 5 cut(s) 355, 668, 671, 997, 1041
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 672, 1175
BstYI RGATCY 2 cut(s) 672, 1175
BsuRI GGCC 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 1253
BtsCI GGATG 2 cut(s) 266, 1003
BtsIMutI CAGTG 2 cut(s) 395, 1310
Cac8I GCNNGC 2 cut(s) 990, 1215
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 3 cut(s) 175, 280, 725
Csp6I GTAC 2 cut(s) 419, 1328
CspAI ACCGGT 1 cut(s) 282
CviAII CATG 5 cut(s) 141, 360, 539, 678, 1214
CviQI GTAC 2 cut(s) 419, 1328
DdeI CTNAG 2 cut(s) 95, 511
DpnI GATC 3 cut(s) 674, 1177, 1344
DpnII GATC 3 cut(s) 672, 1175, 1342
DrdI GACNNNNNNGTC 1 cut(s) 99
DseDI GACNNNNNNGTC 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 1165
Eco24I GRGCYC 1 cut(s) 1167
Eco31I GGTCTC 1 cut(s) 561
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 175, 280
Eco53kI GAGCTC 1 cut(s) 1165
Eco88I CYCGRG 1 cut(s) 814
EcoICRI GAGCTC 1 cut(s) 1165
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 624
EcoRV GATATC 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 1167
FaeI CATG 5 cut(s) 144, 363, 542, 681, 1217
FalI AAGNNNNNCTT 2 cut(s) 739, 771
FaqI GGGAC 2 cut(s) 575, 784
FatI CATG 5 cut(s) 140, 359, 538, 677, 1213
Fnu4HI GCNGC 5 cut(s) 344, 682, 685, 986, 1030
FokI GGATG 2 cut(s) 253, 1010
FriOI GRGCYC 1 cut(s) 1167
Fsp4HI GCNGC 5 cut(s) 344, 682, 685, 986, 1030
FspBI CTAG 4 cut(s) 33, 443, 789, 1083
FspI TGCGCA 1 cut(s) 145
GlaI GCGC 1 cut(s) 145
GluI GCNGC 5 cut(s) 344, 682, 685, 986, 1030
GsuI CTGGAG 1 cut(s) 647
HaeIII GGCC 1 cut(s) 727
HapII CCGG 1 cut(s) 283
HhaI GCGC 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 5 cut(s) 144, 363, 542, 681, 1217
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HincII GTYRAC 2 cut(s) 228, 633
HindII GTYRAC 2 cut(s) 228, 633
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 5 cut(s) 545, 776, 857, 931, 1185
HpaII CCGG 1 cut(s) 283
HphI GGTGA 4 cut(s) 59, 730, 830, 926
Hpy166II GTNNAC 8 cut(s) 228, 433, 633, 849, 888, 1155, 1246, 1328
Hpy188I TCNGA 4 cut(s) 234, 379, 561, 1264
Hpy188III TCNNGA 5 cut(s) 107, 710, 899, 962, 1208
Hpy8I GTNNAC 8 cut(s) 228, 433, 633, 849, 888, 1155, 1246, 1328
HpyAV CCTTC 5 cut(s) 44, 53, 377, 607, 1166
HpyCH4III ACNGT 2 cut(s) 374, 390
HpyCH4IV ACGT 4 cut(s) 635, 851, 1011, 1036
HpyF10VI GCNNNNNNNGC 1 cut(s) 690
HpyF3I CTNAG 2 cut(s) 95, 511
HpySE526I ACGT 4 cut(s) 635, 851, 1011, 1036
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 5 cut(s) 144, 363, 542, 681, 1217
HspAI GCGC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 672, 1175, 1342
LmnI GCTCC 1 cut(s) 1117
Lsp1109I GCAGC 5 cut(s) 355, 668, 671, 997, 1041
LweI GCATC 3 cut(s) 130, 197, 540
MaeI CTAG 4 cut(s) 33, 443, 789, 1083
MaeII ACGT 4 cut(s) 635, 851, 1011, 1036
MaeIII GTNAC 1 cut(s) 640
MalI GATC 3 cut(s) 674, 1177, 1344
MboI GATC 3 cut(s) 672, 1175, 1342
MboII GAAGA 5 cut(s) 95, 494, 600, 771, 977
MfeI CAATTG 2 cut(s) 688, 1158
MflI RGATCY 2 cut(s) 672, 1175
MhlI GDGCHC 1 cut(s) 1167
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 6 cut(s) 106, 188, 373, 546, 604, 649
MseI TTAA 6 cut(s) 12, 828, 909, 1074, 1089, 1320
MslI CAYNNNNRTG 1 cut(s) 1263
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 626
MunI CAATTG 2 cut(s) 688, 1158
Mva1269I GAATGC 1 cut(s) 1258
MvaI CCWGG 1 cut(s) 626
MwoI GCNNNNNNNGC 1 cut(s) 690
NdeII GATC 3 cut(s) 672, 1175, 1342
NlaIII CATG 5 cut(s) 144, 363, 542, 681, 1217
NsbI TGCGCA 1 cut(s) 145
NspI RCATGY 1 cut(s) 1217
PaeI GCATGC 1 cut(s) 1217
PctI GAATGC 1 cut(s) 1258
PfeI GAWTC 5 cut(s) 545, 776, 857, 931, 1185
PinAI ACCGGT 1 cut(s) 282
PkrI GCNGC 5 cut(s) 345, 683, 686, 987, 1031
Ppu21I YACGTR 1 cut(s) 1037
PpuMI RGGWCCY 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 1167
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 624
PspGI CCWGG 1 cut(s) 624
PspPI GGNCC 3 cut(s) 175, 280, 725
PspPPI RGGWCCY 1 cut(s) 175
PsrI GAACNNNNNNTAC 2 cut(s) 1005, 1037
PstI CTGCAG 3 cut(s) 987, 994, 1031
PsuI RGATCY 2 cut(s) 672, 1175
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 2 cut(s) 420, 1329
RsaNI GTAC 2 cut(s) 419, 1328
RseI CAYNNNNRTG 1 cut(s) 1263
SacI GAGCTC 1 cut(s) 1167
SaqAI TTAA 6 cut(s) 12, 828, 909, 1074, 1089, 1320
SatI GCNGC 5 cut(s) 344, 682, 685, 986, 1030
Sau3AI GATC 3 cut(s) 672, 1175, 1342
Sau96I GGNCC 3 cut(s) 175, 280, 725
SbfI CCTGCAGG 1 cut(s) 994
ScaI AGTACT 1 cut(s) 420
ScrFI CCNGG 1 cut(s) 626
SdaI CCTGCAGG 1 cut(s) 994
SduI GDGCHC 1 cut(s) 1167
SfaNI GCATC 3 cut(s) 130, 197, 540
SfcI CTRYAG 6 cut(s) 645, 840, 983, 990, 1027, 1145
SinI GGWCC 2 cut(s) 175, 280
SmiMI CAYNNNNRTG 1 cut(s) 1263
SmlI CTYRAG 2 cut(s) 550, 1208
SmoI CTYRAG 2 cut(s) 550, 1208
SphI GCATGC 1 cut(s) 1217
Sse8387I CCTGCAGG 1 cut(s) 994
SspMI CTAG 4 cut(s) 33, 443, 789, 1083
SstI GAGCTC 1 cut(s) 1167
StyD4I CCNGG 1 cut(s) 624
TaaI ACNGT 2 cut(s) 374, 390
TaiI ACGT 4 cut(s) 638, 854, 1014, 1039
TaqI TCGA 3 cut(s) 200, 415, 1188
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 5 cut(s) 545, 776, 857, 931, 1185
Tru1I TTAA 6 cut(s) 12, 828, 909, 1074, 1089, 1320
Tru9I TTAA 6 cut(s) 12, 828, 909, 1074, 1089, 1320
TscAI CASTG 2 cut(s) 395, 1317
TseI GCWGC 5 cut(s) 343, 681, 684, 985, 1029
TspDTI ATGAA 4 cut(s) 348, 357, 527, 944
TspGWI ACGGA 2 cut(s) 246, 1076
TspRI CASTG 2 cut(s) 395, 1317
VpaK11BI GGWCC 2 cut(s) 175, 280
XapI RAATTY 6 cut(s) 582, 922, 950, 1097, 1109, 1222
XceI RCATGY 1 cut(s) 1217
XspI CTAG 4 cut(s) 33, 443, 789, 1083
ZraI GACGTC 1 cut(s) 636
ZrmI AGTACT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.