Rroxscaffold_6G00407570

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000241
Physical Location & Seq
Reverse (-)
30191030 .. 30193297
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00407570.1

Sequence Viewer

Length: 1491 bp
ATGGAGCAGAAGCGTATGTTATTGGCTACTGGAAGTTGTGAGGGTGAAGGACCCTGTACTGTGAATATGATTGACAGAGTTAGCAGTCTTCCGGAGGGAGTTTTTCATCACATTCTTTCAAAATTTGGTATTAAAGACCTTGCTCGGTTCTGCTGTGTCTCCAAACGATGGAGAGAATTATGCCTGTCATCTCCATCGGTGGAATTTTGTGGATTTAGTGAAGTTGGAATTATGGATATGGCATGTGAGCTTAGGTTGAAGTTGGTGAATGCATTGGATAGGTTCTTGCTTAGTCGTGGGGATAATGAGATGAAGAGTTTTGTTCTTTTTTGGGATGGTCACAGTGATGAAGAGCTTGACGAAGCATGTTTCTGTGTTAATGAGAATTTCCGAATAATCACCTGGATCCAGAATGCTGTGAGGTGTAAAGTTGAAAAGGTTTATCTTGACGTCACCTTTTTTGATTATGAGGGGGAGCCATTGGCATTTCCATCTTGTGTCTTTCGTTCTGCATCATTGAGGTCTATAGTGGTTAATATGCCACGTACAGTTGTTAAAACACCCTCCTTCACTTTTTCCTCCAATATCGAAAAGTTGCATTTAAGCGATGTTGTTATACAGGACGAGCGGTTTTTCAAGTGGATTTCCTATTCCTGCAAATCCTTGAAGGATGTAACTCTTTCAGCAGTTCGTGAGATACATAGTATCACCATTGAAAGCTCGTCTTTGGAAAAATTTTATTTCATTAATTACGAGGTTGATGAAACCTGCCATATTAACATCTCTGGTGAGAAACTTGAAGAAATAAATGTCAGGTGCAATCATTCACCTAGCAGCACCATATTAAAAATTGTTGCTCCAAATGTTAAACATTTGCTTTTGGAGGGGAATGTGAAAAATCACCTAAATCTGGGACAATTGAAATGTTTGGAACAAGCTGCTATTCTTATGGAGCCTATAGTAGATGAGTTTAACAAAGTATTTGAGGTTCTATCCAGTTTGTGCAGTGTTGAAGTTCTTGTTCTAAATGAAGCTACCATCCAGGCTGCATTCAGGGAGGAATGCATGCAAGCTCGATTGGATGAGATTTGGTACTTGCAAATGAATATTGGTAGCTTTACTGATGATCTAGTCCCGGCAGTCGTCTGTCTTTTAAGAGGAACACCAGAGTTGTGTACTTTATACATTCGCTATAAACCAACTTCACTCGACCCTAAATCTAATACATCTGGGTTTGATATGGAGTATTGGAAGATGCAAAACCTGGATTTTGTTTCTCAGGTTGAGGATGTTACCATAGAGCTTAGTGCTGGGTCTAATGGAATTGAGTTAGCAAGGTATATACTTGAGAATGCTGAGAGCCTGGAGAAAATGGTCATTAGGTATTTGGCCGAGCACTCCAATGCTATAGGGAAGTTAAAAGAAAGTAAGATGATCTCCAATCCCTTGGTTACTTTCGAGGAATATGATAGATCAGTTGGTGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

496

Amino Acids

56.47

Weight (kDa)

5.04

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 28 - 65 3.5e-09 F-box domain
F-box-like PF12937 28 - 63 2.1e-07 F-box-like
LRR_At1g61320_AtMIF1 PF23622 130 - 269 2e-06 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 134 - 269 1.3e-06 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 278 - 467 8.1e-07 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000241)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18954 FvH4_6g18982 FvH4_6g19010 FvH4_6g19020 FvH4_6g19020 FvH4_6g19030 FvH4_6g19030 FvH4_6g19040 FvH4_6g19040 FvH4_6g19040 FvH4_6g19051 FvH4_6g19090 FvH4_6g19090 FvH4_6g19090 FvH4_6g33920 FvH4_6g33940
malus_domestica MD00G1221800.v1.1 MD04G1145400.v1.1 MD12G1026400.v1.1 MD12G1026500.v1.1 MD12G1026700.v1.1 MD12G1026800.v1.1 MD14G1024900.v1.1 MD14G1025000.v1.1 MD14G1025100.v1.1 MD14G1025200.v1.1 MD14G1025300.v1.1 MD14G1025400.v1.1 MD14G1025600.v1.1 MD14G1026200.v1.1 MD14G1026700.v1.1
prunus_persica Prupe.7G104800_v2.0.a1 Prupe.7G104900_v2.0.a1 Prupe.7G105000_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105600_v2.0.a1 Prupe.7G105900_v2.0.a1 Prupe.7G106200_v2.0.a1
pyrus_communis pycom04g13160 pycom07g14430 pycom12g02570 pycom14g02380 pycom14g02430 pycom14g02470 pycom14g02480 pycom14g02490
rosa_chinensis RchiOBHm_Chr3g0474001 RchiOBHm_Chr3g0474011 RchiOBHm_Chr3g0474021 RchiOBHm_Chr3g0474031 RchiOBHm_Chr3g0474041 RchiOBHm_Chr3g0474111 RchiOBHm_Chr3g0474131 RchiOBHm_Chr3g0474161 RchiOBHm_Chr3g0474191
rosa_laevigata RLG00000023848 RLG00000023942 RLG00000023944 RLG00000023946 RLG00000023948 RLG00000023956 RLG00000023957 RLG00000023958
rosa_multiflora Rmu_co8028860.1_g000001 Rmu_sc0002414.1_g000007 Rmu_sc0003170.1_g000008 Rmu_sc0003170.1_g000009 Rmu_sc0004167.1_g000001 Rmu_sc0004507.1_g000023 Rmu_sc0004507.1_g000029 Rmu_sc0004507.1_g000030 Rmu_sc0004507.1_g000032 Rmu_sc0004507.1_g000045 Rmu_sc0005137.1_g000004 Rmu_sc0015051.1_g000002 Rmu_sc0027615.1_g000001 Rmu_sc0027615.1_g000002
rosa_roxburghii Rroxscaffold_6G00407440 Rroxscaffold_6G00407450 Rroxscaffold_6G00407470 Rroxscaffold_6G00407550 Rroxscaffold_6G00407560 Rroxscaffold_6G00407570
rosa_rugosa Rorug03G0137600 Rorug03G0137600 Rorug03G0137600 Rorug03G0138800 Rorug03G0138900 Rorug03G0139200 Rorug03G0139300 Rorug03G0139300 Rorug03G0139400 Rorug03G0139500
rosa_samantha Rh3AG187700 Rh3AG187800 Rh3AG187900 Rh3AG188000 Rh3AG188100 Rh3AG188700 Rh3AG188900 Rh3AG189100 Rh3AG189200 Rh3AG189800 Rh3BG216500 Rh3BG216600 Rh3BG216700 Rh3BG216800 Rh3BG216900 Rh3BG218300 Rh3BG218500 Rh3BG219000 Rh3CG212900 Rh3CG213000 Rh3CG213100 Rh3CG213200 Rh3CG214000 Rh3CG214200 Rh3CG214400 Rh3CG222800 Rh3DG212300 Rh3DG212400 Rh3DG212500 Rh3DG212600 Rh3DG212700 Rh3DG213200 Rh3DG213500 Rh3DG213700 Rh3DG213800 Rh3DG214400 Rh3DG214500 Rh3DG223800
rosa_wichuraiana Rw3G017170 Rw3G017200 Rw3G017210 Rw3G017220 Rw3G017230 Rw3G017290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 453
Acc36I ACCTGC 1 cut(s) 776
AccB7I CCANNNNNTGG 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 628
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 1 cut(s) 628
AclWI GGATC 2 cut(s) 400, 413
AcoI YGGCCR 1 cut(s) 1389
AcsI RAATTY 4 cut(s) 122, 203, 385, 734
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 4 cut(s) 58, 547, 1094, 1177
AfiI CCNNNNNNNGG 2 cut(s) 168, 910
AgsI TTSAA 9 cut(s) 120, 259, 434, 637, 667, 716, 800, 922, 1013
AjnI CCWGG 4 cut(s) 401, 1041, 1263, 1362
AluBI AGCT 8 cut(s) 250, 355, 720, 938, 1034, 1073, 1116, 1303
AluI AGCT 8 cut(s) 250, 355, 720, 938, 1034, 1073, 1116, 1303
Alw21I GWGCWC 1 cut(s) 1398
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 2 cut(s) 400, 413
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 1 cut(s) 1389
ApeKI GCWGC 3 cut(s) 834, 938, 1046
ApoI RAATTY 4 cut(s) 122, 203, 385, 734
AseI ATTAAT 1 cut(s) 747
AspS9I GGNCC 1 cut(s) 50
AsuC2I CCSGG 1 cut(s) 1136
AsuHPI GGTGA 8 cut(s) 56, 277, 391, 445, 700, 800, 819, 893
AvaII GGWCC 1 cut(s) 50
BamHI GGATCC 1 cut(s) 405
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 1398
BbvI GCAGC 3 cut(s) 846, 925, 1033
BccI CCATC 5 cut(s) 162, 202, 329, 499, 1046
BciT130I CCWGG 4 cut(s) 403, 1043, 1265, 1364
BcnI CCSGG 1 cut(s) 1136
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 2 cut(s) 831, 1130
BfmI CTRYAG 3 cut(s) 525, 957, 1407
BfuAI ACCTGC 1 cut(s) 776
BisI GCNGC 3 cut(s) 835, 939, 1047
BlsI GCNGC 3 cut(s) 836, 940, 1048
Bme1390I CCNGG 5 cut(s) 403, 1043, 1136, 1265, 1364
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 4 cut(s) 52, 407, 477, 954
BmrFI CCNGG 5 cut(s) 403, 1043, 1136, 1265, 1364
BmsI GCATC 2 cut(s) 521, 1245
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 1385
Bpu10I CCTNAGC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 1367
BpuMI CCSGG 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 1446
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 910
Bse1I ACTGG 2 cut(s) 34, 996
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 4 cut(s) 403, 1043, 1265, 1364
BseDI CCNNGG 1 cut(s) 1446
BseGI GGATG 5 cut(s) 340, 676, 1038, 1087, 1294
BseLI CCNNNNNNNGG 2 cut(s) 168, 910
BseMII CTCAG 2 cut(s) 1292, 1347
BseNI ACTGG 2 cut(s) 34, 996
BseXI GCAGC 3 cut(s) 846, 925, 1033
BseYI CCCAGC 1 cut(s) 1310
BsgI GTGCAG 1 cut(s) 1024
BshFI GGCC 1 cut(s) 1391
BsiHKAI GWGCWC 1 cut(s) 1398
BsiSI CCGG 2 cut(s) 92, 1136
BslFI GGGAC 2 cut(s) 927, 1118
BslI CCNNNNNNNGG 2 cut(s) 168, 910
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 927, 1118
BsmI GAATGC 5 cut(s) 274, 418, 1049, 1067, 1357
BsnI GGCC 1 cut(s) 1391
Bsp1286I GDGCHC 1 cut(s) 1398
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 405, 1126, 1434, 1472
BspACI CCGC 1 cut(s) 628
BspANI GGCC 1 cut(s) 1391
BspCNI CTCAG 2 cut(s) 1291, 1348
BspEI TCCGGA 1 cut(s) 91
BspLI GGNNCC 4 cut(s) 52, 407, 477, 954
BspMI ACCTGC 1 cut(s) 776
BspPI GGATC 2 cut(s) 400, 413
BspQI GCTCTTC 1 cut(s) 345
BsrBI CCGCTC 1 cut(s) 628
BsrI ACTGG 2 cut(s) 34, 996
BssECI CCNNGG 1 cut(s) 1446
BssMI GATC 4 cut(s) 405, 1126, 1434, 1472
BssNI GRCGYC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 1446
Bst2UI CCWGG 4 cut(s) 403, 1043, 1265, 1364
Bst4CI ACNGT 3 cut(s) 61, 344, 550
Bst6I CTCTTC 2 cut(s) 308, 345
BstACI GRCGYC 1 cut(s) 450
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 1067, 1071
BstDEI CTNAG 5 cut(s) 251, 290, 1278, 1304, 1356
BstF5I GGATG 5 cut(s) 340, 676, 1038, 1087, 1294
BstKTI GATC 4 cut(s) 408, 1129, 1437, 1475
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 405, 1126, 1434, 1472
BstNI CCWGG 4 cut(s) 403, 1043, 1265, 1364
BstNSI RCATGY 3 cut(s) 246, 369, 1069
BstSCI CCNGG 5 cut(s) 401, 1041, 1134, 1263, 1362
BstSFI CTRYAG 3 cut(s) 525, 957, 1407
BstV1I GCAGC 3 cut(s) 846, 925, 1033
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 1447
BstYI RGATCY 1 cut(s) 405
BsuRI GGCC 1 cut(s) 1391
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 5 cut(s) 340, 676, 1038, 1087, 1294
BtsI GCAGTG 1 cut(s) 1012
BtsIMutI CAGTG 2 cut(s) 349, 1012
BveI ACCTGC 1 cut(s) 776
Cac8I GCNNGC 2 cut(s) 1067, 1071
Cfr13I GGNCC 1 cut(s) 50
Csp6I GTAC 4 cut(s) 57, 546, 1093, 1176
CviAII CATG 3 cut(s) 243, 366, 1066
CviQI GTAC 4 cut(s) 57, 546, 1093, 1176
DdeI CTNAG 5 cut(s) 251, 290, 1278, 1304, 1356
DpnI GATC 4 cut(s) 407, 1128, 1436, 1474
DpnII GATC 4 cut(s) 405, 1126, 1434, 1472
EaeI YGGCCR 1 cut(s) 1389
Eam1104I CTCTTC 2 cut(s) 308, 345
EarI CTCTTC 2 cut(s) 308, 345
Eco130I CCWWGG 1 cut(s) 1446
Eco47I GGWCC 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 4 cut(s) 401, 1041, 1263, 1362
EcoT14I CCWWGG 1 cut(s) 1446
EcoT22I ATGCAT 2 cut(s) 274, 1067
ErhI CCWWGG 1 cut(s) 1446
FaeI CATG 3 cut(s) 246, 369, 1069
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FaqI GGGAC 2 cut(s) 927, 1118
FatI CATG 3 cut(s) 242, 365, 1065
Fnu4HI GCNGC 3 cut(s) 835, 939, 1047
FokI GGATG 5 cut(s) 347, 683, 1025, 1094, 1301
Fsp4HI GCNGC 3 cut(s) 835, 939, 1047
FspBI CTAG 2 cut(s) 831, 1130
GluI GCNGC 3 cut(s) 835, 939, 1047
GsaI CCCAGC 1 cut(s) 1314
GsuI CTGGAG 1 cut(s) 1385
HaeIII GGCC 1 cut(s) 1391
HapII CCGG 2 cut(s) 92, 1136
Hin1I GRCGYC 1 cut(s) 450
Hin1II CATG 3 cut(s) 246, 369, 1069
HpaII CCGG 2 cut(s) 92, 1136
HphI GGTGA 8 cut(s) 56, 277, 391, 445, 700, 800, 819, 893
Hpy166II GTNNAC 1 cut(s) 1176
Hpy188I TCNGA 1 cut(s) 392
Hpy188III TCNNGA 4 cut(s) 92, 409, 446, 692
Hpy8I GTNNAC 1 cut(s) 1176
HpyAV CCTTC 3 cut(s) 41, 577, 661
HpyCH4III ACNGT 3 cut(s) 61, 344, 550
HpyCH4IV ACGT 2 cut(s) 450, 544
HpyF3I CTNAG 5 cut(s) 251, 290, 1278, 1304, 1356
HpySE526I ACGT 2 cut(s) 450, 544
Hsp92I GRCGYC 1 cut(s) 450
Hsp92II CATG 3 cut(s) 246, 369, 1069
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 4 cut(s) 405, 1126, 1434, 1472
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 4 cut(s) 4, 475, 862, 952
Lsp1109I GCAGC 3 cut(s) 846, 925, 1033
LweI GCATC 2 cut(s) 521, 1245
MaeI CTAG 2 cut(s) 831, 1130
MaeII ACGT 2 cut(s) 450, 544
MaeIII GTNAC 5 cut(s) 338, 451, 673, 1291, 1450
MalI GATC 4 cut(s) 407, 1128, 1436, 1474
MbiI CCGCTC 1 cut(s) 628
MboI GATC 4 cut(s) 405, 1126, 1434, 1472
MboII GAAGA 5 cut(s) 80, 325, 362, 812, 1264
MfeI CAATTG 1 cut(s) 917
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 1 cut(s) 1398
MmeI TCCRAC 1 cut(s) 205
Mph1103I ATGCAT 2 cut(s) 274, 1067
MroI TCCGGA 1 cut(s) 91
MslI CAYNNNNRTG 2 cut(s) 345, 1401
MspI CCGG 2 cut(s) 92, 1136
MspR9I CCNGG 5 cut(s) 403, 1043, 1136, 1265, 1364
MunI CAATTG 1 cut(s) 917
Mva1269I GAATGC 5 cut(s) 274, 418, 1049, 1067, 1357
MvaI CCWGG 4 cut(s) 403, 1043, 1265, 1364
NciI CCSGG 1 cut(s) 1136
NdeII GATC 4 cut(s) 405, 1126, 1434, 1472
NlaIII CATG 3 cut(s) 246, 369, 1069
NlaIV GGNNCC 4 cut(s) 52, 407, 477, 954
NmeAIII GCCGAG 1 cut(s) 1417
NmuCI GTSAC 2 cut(s) 338, 451
NsiI ATGCAT 2 cut(s) 274, 1067
NspI RCATGY 3 cut(s) 246, 369, 1069
PaeI GCATGC 1 cut(s) 1069
PciSI GCTCTTC 1 cut(s) 345
PctI GAATGC 5 cut(s) 274, 418, 1049, 1067, 1357
PflMI CCANNNNNTGG 1 cut(s) 168
PkrI GCNGC 3 cut(s) 836, 940, 1048
Ppu21I YACGTR 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 4 cut(s) 401, 1041, 1263, 1362
PspFI CCCAGC 1 cut(s) 1310
PspGI CCWGG 4 cut(s) 401, 1041, 1263, 1362
PspN4I GGNNCC 4 cut(s) 52, 407, 477, 954
PspPI GGNCC 1 cut(s) 50
PspPPI RGGWCCY 1 cut(s) 50
PsrI GAACNNNNNNTAC 2 cut(s) 972, 1004
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 4 cut(s) 58, 547, 1094, 1177
RsaNI GTAC 4 cut(s) 57, 546, 1093, 1176
RseI CAYNNNNRTG 2 cut(s) 345, 1401
SapI GCTCTTC 1 cut(s) 345
SatI GCNGC 3 cut(s) 835, 939, 1047
Sau3AI GATC 4 cut(s) 405, 1126, 1434, 1472
Sau96I GGNCC 1 cut(s) 50
ScrFI CCNGG 5 cut(s) 403, 1043, 1136, 1265, 1364
SduI GDGCHC 1 cut(s) 1398
SfaNI GCATC 2 cut(s) 521, 1245
SfcI CTRYAG 3 cut(s) 525, 957, 1407
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 345, 1401
SmlI CTYRAG 1 cut(s) 1346
SmoI CTYRAG 1 cut(s) 1346
SphI GCATGC 1 cut(s) 1069
SsiI CCGC 1 cut(s) 628
SspI AATATT 1 cut(s) 1108
SspMI CTAG 2 cut(s) 831, 1130
StyD4I CCNGG 5 cut(s) 401, 1041, 1134, 1263, 1362
StyI CCWWGG 1 cut(s) 1446
TaaI ACNGT 3 cut(s) 61, 344, 550
TaiI ACGT 2 cut(s) 453, 547
TaqI TCGA 4 cut(s) 588, 1075, 1209, 1458
TatI WGTACW 2 cut(s) 56, 1175
TscAI CASTG 2 cut(s) 349, 1012
TseFI GTSAC 2 cut(s) 338, 451
TseI GCWGC 3 cut(s) 834, 938, 1046
Tsp45I GTSAC 2 cut(s) 338, 451
TspDTI ATGAA 7 cut(s) 95, 326, 363, 733, 777, 1044, 1118
TspRI CASTG 2 cut(s) 349, 1012
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 50
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 4 cut(s) 122, 203, 385, 734
XceI RCATGY 3 cut(s) 246, 369, 1069
XspI CTAG 2 cut(s) 831, 1130
ZraI GACGTC 1 cut(s) 451
Zsp2I ATGCAT 2 cut(s) 274, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.