Rw3G017230

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000241
Physical Location & Seq
Forward (+)
18646386 .. 18648431
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017230.1

Sequence Viewer

Length: 1467 bp
ATGGAAATGATGAAACAAAGGCGTAAGTTAGCAAGTTGTGAAGGTGTAGGAGGTAGTGGTGGGAATGAGAGCGGGATAGACAGATTTAGCGATCTTCCCGATCAAGTTGCACACCACATTCTTTCCTTCCTGGCTTTGCCGGACCTTAGTCGTGTGTGTTGTGTGTCCAAAAGGTGTGGAGAACTTTGTATATCTGCTCCCTCACTGAATTTTGATGAATTTTCCTCGGATTCTATGTCCACGTGTTCCGGGCGGCTAAAGTTGTTGACTTATTTGGAAAGGTTCTTGTTTCATCGTGGGGATAATAAGATACAGTCCTTTCGTCTCCGTTGGGAAAGTCACTCTATGGATGTAGATTTAACACCATGCATCTGTGATAATGAGTGTTATCGGTTGAGCACTTGGATCCAGAATGCAGTACGGTGTAATGTTGAAATGCTTGGTCTTAAGATTACTTTATATAACACCGAATTGGTGTTTTTTCCATCTTGTGTGTTTCTCTGTGCACCTTTGAAGTCTCTGGTGGTGGACATGAATTATACGGTCCTTAGAACTCCGTCTTTTGCCTTTTCCTCTAATCTCAAGTACTTGCAGTTGACAAATGTTTATATAGAAGATGACAAGTTTTTCAAATGGATCTCATGTTCCTGCAAGTGCATTGGGGATTTGCATCTTAGTCATGTTCTTGGGATAGAGAACGTCACCATTTTCCGGTCATCTCTGGAAAGGTTTAGTTTGATTTGTGGTCCTCTGGATCATATGTGCCATTTAAATATCTCAAGTGAGAAACTTGTGGACATAGATCTTTACTGGACATTTGATTCACCCAGCAACAAATCCTTAACTATTGATGCCCCAAATCTTAAATGTTTCAAATGGATTGGGAATTTGATGAAACACCCAAATCTGGGAAAACTAGAATGCTTAGAAACAGCTTCACTTTTTCTGCTGCCTGAAGTAGAGGACTTAAACAAGGTACATGAGGTTCTTTGGAGTTTACGCAGGGCTCCAGCTCTTTTGCTAAATGAAGTGGTGATTAAGGCTTCATTTAGGGATGTATCTGTGCCAGCTTCAATGTATGATACTTCTTATTTGTGTATATGTATCGAAAGCTTTGTTGATGAGCTGGTCCCGGCATTGGTCTGTGTCTTCAGAAGAACACCTAATTTGAGTTTGTTATGCATAAAGTCTAAACCACCACATGACCCTCAGTCTAATACATTCGGGTTTAATATGGAATACTGGAAGCTGCAAGACCTTGCTTTTACTAATCAGCTTAAGCATGTGACTTTAGAGCTTTGCAGTGGGTCTAATGGAATTGAGTTGGCAAAGTATATGCTCCAGTATGCTCTCAATATGCAGAGATTGGTTGTGATTTGTTTGCCCATAGATCTGGAGGAAGTTACAAGGAAACTAAAGAAAATCAAGATGATTTCCAATGCTGTACTTGTCATTCAGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

488

Amino Acids

55.55

Weight (kDa)

6.84

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000241)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18954 FvH4_6g18982 FvH4_6g19010 FvH4_6g19020 FvH4_6g19020 FvH4_6g19030 FvH4_6g19030 FvH4_6g19040 FvH4_6g19040 FvH4_6g19040 FvH4_6g19051 FvH4_6g19090 FvH4_6g19090 FvH4_6g19090 FvH4_6g33920 FvH4_6g33940
malus_domestica MD00G1221800.v1.1 MD04G1145400.v1.1 MD12G1026400.v1.1 MD12G1026500.v1.1 MD12G1026700.v1.1 MD12G1026800.v1.1 MD14G1024900.v1.1 MD14G1025000.v1.1 MD14G1025100.v1.1 MD14G1025200.v1.1 MD14G1025300.v1.1 MD14G1025400.v1.1 MD14G1025600.v1.1 MD14G1026200.v1.1 MD14G1026700.v1.1
prunus_persica Prupe.7G104800_v2.0.a1 Prupe.7G104900_v2.0.a1 Prupe.7G105000_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105500_v2.0.a1 Prupe.7G105600_v2.0.a1 Prupe.7G105900_v2.0.a1 Prupe.7G106200_v2.0.a1
pyrus_communis pycom04g13160 pycom07g14430 pycom12g02570 pycom14g02380 pycom14g02430 pycom14g02470 pycom14g02480 pycom14g02490
rosa_chinensis RchiOBHm_Chr3g0474001 RchiOBHm_Chr3g0474011 RchiOBHm_Chr3g0474021 RchiOBHm_Chr3g0474031 RchiOBHm_Chr3g0474041 RchiOBHm_Chr3g0474111 RchiOBHm_Chr3g0474131 RchiOBHm_Chr3g0474161 RchiOBHm_Chr3g0474191
rosa_laevigata RLG00000023848 RLG00000023942 RLG00000023944 RLG00000023946 RLG00000023948 RLG00000023956 RLG00000023957 RLG00000023958
rosa_multiflora Rmu_co8028860.1_g000001 Rmu_sc0002414.1_g000007 Rmu_sc0003170.1_g000008 Rmu_sc0003170.1_g000009 Rmu_sc0004167.1_g000001 Rmu_sc0004507.1_g000023 Rmu_sc0004507.1_g000029 Rmu_sc0004507.1_g000030 Rmu_sc0004507.1_g000032 Rmu_sc0004507.1_g000045 Rmu_sc0005137.1_g000004 Rmu_sc0015051.1_g000002 Rmu_sc0027615.1_g000001 Rmu_sc0027615.1_g000002
rosa_roxburghii Rroxscaffold_6G00407440 Rroxscaffold_6G00407450 Rroxscaffold_6G00407470 Rroxscaffold_6G00407550 Rroxscaffold_6G00407560 Rroxscaffold_6G00407570
rosa_rugosa Rorug03G0137600 Rorug03G0137600 Rorug03G0137600 Rorug03G0138800 Rorug03G0138900 Rorug03G0139200 Rorug03G0139300 Rorug03G0139300 Rorug03G0139400 Rorug03G0139500
rosa_samantha Rh3AG187700 Rh3AG187800 Rh3AG187900 Rh3AG188000 Rh3AG188100 Rh3AG188700 Rh3AG188900 Rh3AG189100 Rh3AG189200 Rh3AG189800 Rh3BG216500 Rh3BG216600 Rh3BG216700 Rh3BG216800 Rh3BG216900 Rh3BG218300 Rh3BG218500 Rh3BG219000 Rh3CG212900 Rh3CG213000 Rh3CG213100 Rh3CG213200 Rh3CG214000 Rh3CG214200 Rh3CG214400 Rh3CG222800 Rh3DG212300 Rh3DG212400 Rh3DG212500 Rh3DG212600 Rh3DG212700 Rh3DG213200 Rh3DG213500 Rh3DG213700 Rh3DG213800 Rh3DG214400 Rh3DG214500 Rh3DG223800
rosa_wichuraiana Rw3G017170 Rw3G017200 Rw3G017210 Rw3G017220 Rw3G017230 Rw3G017290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 72
AciI CCGC 2 cut(s) 72, 253
AclWI GGATC 4 cut(s) 400, 413, 644, 762
AcsI RAATTY 3 cut(s) 208, 218, 886
AcuI CTGAAG 2 cut(s) 975, 1135
AcvI CACGTG 1 cut(s) 243
AfaI GTAC 4 cut(s) 420, 587, 978, 1446
AfiI CCNNNNNNNGG 4 cut(s) 711, 907, 908, 1138
AflII CTTAAG 2 cut(s) 446, 1277
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 5 cut(s) 434, 514, 631, 874, 1074
AhdI GACNNNNNGTC 1 cut(s) 1210
AjnI CCWGG 1 cut(s) 129
AloI GAACNNNNNNTCC 2 cut(s) 628, 660
AluBI AGCT 8 cut(s) 935, 1013, 1070, 1113, 1126, 1249, 1276, 1297
AluI AGCT 8 cut(s) 935, 1013, 1070, 1113, 1126, 1249, 1276, 1297
Alw21I GWGCWC 2 cut(s) 401, 508
Alw26I GTCTC 2 cut(s) 329, 522
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 4 cut(s) 400, 413, 644, 762
ApaLI GTGCAC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 949, 1249
ApoI RAATTY 3 cut(s) 208, 218, 886
AspS9I GGNCC 4 cut(s) 142, 544, 746, 1129
AsuC2I CCSGG 2 cut(s) 250, 1133
AsuHPI GGTGA 3 cut(s) 694, 816, 1045
AvaII GGWCC 4 cut(s) 142, 544, 746, 1129
BaeGI GKGCMC 1 cut(s) 508
BamHI GGATCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 1009
BbrPI CACGTG 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 1141
Bbv12I GWGCWC 2 cut(s) 401, 508
BbvI GCAGC 2 cut(s) 936, 1236
BccI CCATC 1 cut(s) 493
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 1 cut(s) 131
BcnI CCSGG 2 cut(s) 250, 1133
BcoDI GTCTC 2 cut(s) 329, 522
BfaI CTAG 1 cut(s) 917
BfrI CTTAAG 2 cut(s) 446, 1277
BglII AGATCT 2 cut(s) 802, 1390
BisI GCNGC 3 cut(s) 254, 950, 1250
BlsI GCNGC 3 cut(s) 255, 951, 1251
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 3 cut(s) 131, 250, 1133
Bme18I GGWCC 4 cut(s) 142, 544, 746, 1129
BmeRI GACNNNNNGTC 1 cut(s) 1210
BmgT120I GGNCC 4 cut(s) 142, 544, 746, 1129
BmiI GGNNCC 3 cut(s) 407, 1008, 1131
BmrFI CCNGG 3 cut(s) 131, 250, 1133
BmsI GCATC 3 cut(s) 378, 679, 841
BoxI GACNNNNGTC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 1141
BpmI CTGGAG 3 cut(s) 993, 1325, 1415
BpuEI CTTGAG 2 cut(s) 566, 763
BpuMI CCSGG 2 cut(s) 250, 1133
BsaAI YACGTR 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 711
Bsc4I CCNNNNNNNGG 4 cut(s) 711, 907, 908, 1138
Bse1I ACTGG 3 cut(s) 815, 1247, 1342
Bse8I GATNNNNATC 1 cut(s) 669
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 355, 1060
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 4 cut(s) 711, 907, 908, 1138
BseMII CTCAG 1 cut(s) 1223
BseNI ACTGG 3 cut(s) 815, 1247, 1342
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 2 cut(s) 936, 1236
BseYI CCCAGC 1 cut(s) 827
BsiHKAI GWGCWC 2 cut(s) 401, 508
BsiSI CCGG 4 cut(s) 140, 249, 712, 1133
BslFI GGGAC 1 cut(s) 1115
BslI CCNNNNNNNGG 4 cut(s) 711, 907, 908, 1138
BsmAI GTCTC 2 cut(s) 329, 522
BsmBI CGTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 1115
BsmI GAATGC 2 cut(s) 418, 926
Bsp1286I GDGCHC 3 cut(s) 401, 508, 1009
Bsp143I GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
BspACI CCGC 2 cut(s) 72, 253
BspCNI CTCAG 1 cut(s) 1222
BspLI GGNNCC 3 cut(s) 407, 1008, 1131
BspPI GGATC 4 cut(s) 400, 413, 644, 762
BspTI CTTAAG 2 cut(s) 446, 1277
BsrBI CCGCTC 1 cut(s) 72
BsrI ACTGG 3 cut(s) 815, 1247, 1342
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 3 cut(s) 315, 423, 544
BstAFI CTTAAG 2 cut(s) 446, 1277
BstBAI YACGTR 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 1068
BstDEI CTNAG 5 cut(s) 146, 548, 674, 925, 1209
BstF5I GGATG 2 cut(s) 355, 1060
BstKTI GATC 7 cut(s) 94, 103, 408, 639, 757, 805, 1393
BstMAI GTCTC 2 cut(s) 329, 522
BstMBI GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 1286
BstPAI GACNNNNGTC 1 cut(s) 147
BstSCI CCNGG 3 cut(s) 129, 248, 1131
BstSLI GKGCMC 1 cut(s) 508
BstV1I GCAGC 2 cut(s) 936, 1236
BstV2I GAAGAC 1 cut(s) 1141
BstX2I RGATCY 4 cut(s) 405, 636, 802, 1390
BstXI CCANNNNNNTGG 1 cut(s) 1393
BstYI RGATCY 4 cut(s) 405, 636, 802, 1390
BtsCI GGATG 2 cut(s) 355, 1060
BtsI GCAGTG 1 cut(s) 1309
BtsIMutI CAGTG 2 cut(s) 203, 1309
Cac8I GCNNGC 1 cut(s) 1068
Cfr13I GGNCC 4 cut(s) 142, 544, 746, 1129
Csp6I GTAC 4 cut(s) 419, 586, 977, 1445
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 7 cut(s) 366, 532, 642, 680, 980, 1202, 1283
CviQI GTAC 4 cut(s) 419, 586, 977, 1445
DdeI CTNAG 5 cut(s) 146, 548, 674, 925, 1209
DpnI GATC 7 cut(s) 93, 102, 407, 638, 756, 804, 1392
DpnII GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
DraI TTTAAA 1 cut(s) 771
DriI GACNNNNNGTC 1 cut(s) 1210
Eam1105I GACNNNNNGTC 1 cut(s) 1210
Eco24I GRGCYC 1 cut(s) 1009
Eco47I GGWCC 4 cut(s) 142, 544, 746, 1129
Eco57I CTGAAG 2 cut(s) 975, 1135
Eco72I CACGTG 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 371, 1184
EcoT38I GRGCYC 1 cut(s) 1009
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 7 cut(s) 369, 535, 645, 683, 983, 1205, 1286
FaqI GGGAC 1 cut(s) 1115
FatI CATG 7 cut(s) 365, 531, 641, 679, 979, 1201, 1282
FauI CCCGC 1 cut(s) 65
FauNDI CATATG 1 cut(s) 759
Fnu4HI GCNGC 3 cut(s) 254, 950, 1250
FokI GGATG 2 cut(s) 362, 1067
FriOI GRGCYC 1 cut(s) 1009
Fsp4HI GCNGC 3 cut(s) 254, 950, 1250
FspBI CTAG 1 cut(s) 917
GluI GCNGC 3 cut(s) 254, 950, 1250
GsaI CCCAGC 1 cut(s) 831
GsuI CTGGAG 3 cut(s) 993, 1325, 1415
HapII CCGG 4 cut(s) 140, 249, 712, 1133
Hin1II CATG 7 cut(s) 369, 535, 645, 683, 983, 1205, 1286
HincII GTYRAC 2 cut(s) 267, 597
HindII GTYRAC 2 cut(s) 267, 597
HindIII AAGCTT 1 cut(s) 1111
HinfI GANTC 2 cut(s) 230, 821
HpaII CCGG 4 cut(s) 140, 249, 712, 1133
HphI GGTGA 3 cut(s) 694, 816, 1045
Hpy166II GTNNAC 7 cut(s) 240, 267, 506, 529, 597, 796, 998
Hpy188I TCNGA 3 cut(s) 229, 1154, 1458
Hpy188III TCNNGA 6 cut(s) 98, 409, 722, 752, 1394, 1426
Hpy8I GTNNAC 7 cut(s) 240, 267, 506, 529, 597, 796, 998
HpyAV CCTTC 2 cut(s) 35, 136
HpyCH4III ACNGT 3 cut(s) 315, 423, 544
HpyCH4IV ACGT 2 cut(s) 242, 699
HpyF3I CTNAG 5 cut(s) 146, 548, 674, 925, 1209
HpySE526I ACGT 2 cut(s) 242, 699
Hsp92II CATG 7 cut(s) 369, 535, 645, 683, 983, 1205, 1286
Kzo9I GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
LmnI GCTCC 3 cut(s) 202, 1012, 1344
Lsp1109I GCAGC 2 cut(s) 936, 1236
LweI GCATC 3 cut(s) 378, 679, 841
MaeI CTAG 1 cut(s) 917
MaeII ACGT 2 cut(s) 242, 699
MaeIII GTNAC 4 cut(s) 338, 700, 1285, 1402
MalI GATC 7 cut(s) 93, 102, 407, 638, 756, 804, 1392
MbiI CCGCTC 1 cut(s) 72
MboI GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
MboII GAAGA 4 cut(s) 86, 626, 1141, 1167
MflI RGATCY 4 cut(s) 405, 636, 802, 1390
MhlI GDGCHC 3 cut(s) 401, 508, 1009
MluCI AATT 7 cut(s) 208, 218, 470, 535, 886, 1165, 1317
MnlI CCTC 9 cut(s) 44, 211, 235, 583, 759, 955, 976, 1218, 1390
Mph1103I ATGCAT 2 cut(s) 371, 1184
MseI TTAA 9 cut(s) 359, 447, 770, 842, 864, 968, 1038, 1230, 1278
MspCI CTTAAG 2 cut(s) 446, 1277
MspI CCGG 4 cut(s) 140, 249, 712, 1133
MspR9I CCNGG 3 cut(s) 131, 250, 1133
Mva1269I GAATGC 2 cut(s) 418, 926
MvaI CCWGG 1 cut(s) 131
NciI CCSGG 2 cut(s) 250, 1133
NdeI CATATG 1 cut(s) 759
NdeII GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
NlaIII CATG 7 cut(s) 369, 535, 645, 683, 983, 1205, 1286
NlaIV GGNNCC 3 cut(s) 407, 1008, 1131
NmuCI GTSAC 3 cut(s) 338, 700, 1285
NsiI ATGCAT 2 cut(s) 371, 1184
NspI RCATGY 1 cut(s) 1286
PctI GAATGC 2 cut(s) 418, 926
PfeI GAWTC 2 cut(s) 230, 821
PkrI GCNGC 3 cut(s) 255, 951, 1251
PmaCI CACGTG 1 cut(s) 243
PmlI CACGTG 1 cut(s) 243
Ppu21I YACGTR 1 cut(s) 243
PshAI GACNNNNGTC 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 129
PspCI CACGTG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 3 cut(s) 407, 1008, 1131
PspPI GGNCC 4 cut(s) 142, 544, 746, 1129
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PsuI RGATCY 4 cut(s) 405, 636, 802, 1390
RsaI GTAC 4 cut(s) 420, 587, 978, 1446
RsaNI GTAC 4 cut(s) 419, 586, 977, 1445
SaqAI TTAA 9 cut(s) 359, 447, 770, 842, 864, 968, 1038, 1230, 1278
SatI GCNGC 3 cut(s) 254, 950, 1250
Sau3AI GATC 7 cut(s) 91, 100, 405, 636, 754, 802, 1390
Sau96I GGNCC 4 cut(s) 142, 544, 746, 1129
ScaI AGTACT 1 cut(s) 587
ScrFI CCNGG 3 cut(s) 131, 250, 1133
SduI GDGCHC 3 cut(s) 401, 508, 1009
SfaNI GCATC 3 cut(s) 378, 679, 841
SinI GGWCC 4 cut(s) 142, 544, 746, 1129
SmiI ATTTAAAT 1 cut(s) 771
SmlI CTYRAG 4 cut(s) 446, 581, 778, 1277
SmoI CTYRAG 4 cut(s) 446, 581, 778, 1277
Sse9I AATT 7 cut(s) 208, 218, 470, 535, 886, 1165, 1317
SsiI CCGC 2 cut(s) 72, 253
SspMI CTAG 1 cut(s) 917
StyD4I CCNGG 3 cut(s) 129, 248, 1131
SwaI ATTTAAAT 1 cut(s) 771
TaaI ACNGT 3 cut(s) 315, 423, 544
TaiI ACGT 2 cut(s) 245, 702
TaqI TCGA 1 cut(s) 1107
TasI AATT 7 cut(s) 208, 218, 470, 535, 886, 1165, 1317
TatI WGTACW 2 cut(s) 585, 1444
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 2 cut(s) 230, 821
Tru1I TTAA 9 cut(s) 359, 447, 770, 842, 864, 968, 1038, 1230, 1278
Tru9I TTAA 9 cut(s) 359, 447, 770, 842, 864, 968, 1038, 1230, 1278
TscAI CASTG 2 cut(s) 210, 1309
TseFI GTSAC 3 cut(s) 338, 700, 1285
TseI GCWGC 2 cut(s) 949, 1249
Tsp45I GTSAC 3 cut(s) 338, 700, 1285
TspDTI ATGAA 7 cut(s) 26, 231, 281, 548, 908, 1035, 1041
TspGWI ACGGA 2 cut(s) 317, 546
TspRI CASTG 2 cut(s) 210, 1309
Vha464I CTTAAG 2 cut(s) 446, 1277
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 4 cut(s) 142, 544, 746, 1129
XapI RAATTY 3 cut(s) 208, 218, 886
XceI RCATGY 1 cut(s) 1286
XspI CTAG 1 cut(s) 917
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 2 cut(s) 371, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.