MD16G1101500.v1.1

Non-functional NADPH-dependent codeinone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000205
Physical Location & Seq
Reverse (-)
7088931 .. 7092014
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1101500.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGCAGCGGCAAGCCAAATCCCGGAAGTGGTGCTCGGCTCCTCCGCCGGCCCCAAGAGCATGCCCGTCCTCGCCTTCGGCACGGCGGCCGACAACTTACAGCCTGACGCGTTGAAAACGGCGGTGATTGAGGCCATCAAGCTAGGCTACAGGCATTTTGATACAGCTTCCATATATGGCTCCGAGCAGACTCTTGGTGACGCCATTAAAGAAGCTCTAGAACTTGGCCTTGTTGATTCCAGAGACAAACTATTCATCACTTCAAAGCTGTGGAGTAATGATGCTCACCCTGATCTCGTCCTTCCAGCTCTCAAGAAATCACTTCAGAATCTTCAACTGGAGTACCTTGACTTGTATCTGATTCACTGGCCTATCAGTGGCAAGCCAGGAAAATTGGTGGACCCATTAGTTGACCTTATGCCTATGGACTTCAAAGGGGTGTGGGCAGCCATGGAAGAATCTCAAAGACTTGGCCTCACCAAATCCATTGGAGTCAGCAACTTCTCTAGCAAAAAGATTGAAACTTTACTCTCTTTTGCTTCCATTCCTCCCTCAGTAAATCAAGTCGAGATGAGTCCATTCTGGCAACAGAAGAAGCTGAGAGACTTCTGCAAGGCCAATGGTATAGTTGTGATTGCATTCTCTCCTCTGGGTGCAATTGGTTCCAGTTGGGGCACCAATCATGTTTTGGAAAGCAAAGTGCTTCAGGATATAGCAGAGGCTCGGGGAAAGACTATTGCTCAGGTTTGCATTAGATGGGTATACCAGGTAGGGGCAACTCTAGCTGTGAAGAGCTACAACAAGGAGAGGTTGAAGCAGAATCTGCTTGTGTTTGACTGGGATCTATCAGAAGATGATCTTGCCAAGATCAATCAAATCCCACAGAAGAAGATGATGCTTAAAGAAAACCTGGTTTCAGCTGATGGATCATCTCCGTACAAGTCTGTTGAAGAATTATGGGATGGAGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

325

Amino Acids

35.66

Weight (kDa)

5.7

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 26 - 293 7.4e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59950 AT1G59960
fragaria_vesca FvH4_2g01280 FvH4_3g20300 FvH4_3g20300 FvH4_4g25591 FvH4_4g25600 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25612 FvH4_4g25612 FvH4_4g25620 FvH4_4g35840
malus_domestica MD04G1063200.v1.1 MD10G1055100.v1.1 MD13G1014700.v1.1 MD13G1101200.v1.1 MD16G1101500.v1.1
prunus_persica Prupe.1G244700_v2.0.a1 Prupe.1G245100_v2.0.a1 Prupe.1G245400_v2.0.a1 Prupe.1G338900_v2.0.a1 Prupe.5G239400_v2.0.a1
pyrus_communis pycom13g08830 pycom16g01150 pycom16g08620
rosa_chinensis RchiOBHm_Chr1g0329311 RchiOBHm_Chr3g0483001 RchiOBHm_Chr4g0395321 RchiOBHm_Chr4g0433021 RchiOBHm_Chr4g0445371 RchiOBHm_Chr4g0445381 RchiOBHm_Chr4g0445421 RchiOBHm_Chr5g0023771 RchiOBHm_Chr5g0023781 RchiOBHm_Chr5g0068101 RchiOBHm_Chr5g0068111 RchiOBHm_Chr6g0281251 RchiOBHm_Chr7g0225361
rosa_laevigata RLG00000001827 RLG00000005742 RLG00000005743 RLG00000005744 RLG00000006791 RLG00000009629 RLG00000012981 RLG00000023308 RLG00000029902 RLG00000032738 RLG00000032739 RLG00000035956 RLG00000035957
rosa_multiflora Rmu_co7982306.1_g000001 Rmu_co8108716.1_g000001 Rmu_co8139310.1_g000001 Rmu_co8479199.1_g000001 Rmu_sc0000050.1_g000005 Rmu_sc0000050.1_g000006 Rmu_sc0000847.1_g000048 Rmu_sc0001372.1_g000008 Rmu_sc0001762.1_g000035 Rmu_sc0002791.1_g000001 Rmu_sc0002791.1_g000002 Rmu_sc0002791.1_g000005 Rmu_sc0003252.1_g000001 Rmu_sc0003351.1_g000013 Rmu_sc0006184.1_g000008 Rmu_sc0009518.1_g000006 Rmu_sc0010817.1_g000030 Rmu_sc0016867.1_g000002 Rmu_ssc0000027.1_g000010
rosa_roxburghii Rroxscaffold_1G00012480 Rroxscaffold_1G00012490 Rroxscaffold_1G00013240 Rroxscaffold_1G00013250 Rroxscaffold_1G00055410 Rroxscaffold_1G00055420 Rroxscaffold_3G00233890 Rroxscaffold_4G00321220 Rroxscaffold_5G00340230 Rroxscaffold_5G00340260 Rroxscaffold_6G00398750 Rroxscaffold_7G00187200
rosa_rugosa Rorug01G0078500 Rorug03G0202900 Rorug03G0358000 Rorug04G0361100 Rorug04G0361200 Rorug05G0079600 Rorug06G0140100 Rorug07G0228800
rosa_samantha Rh1AG096900 Rh1BG077500 Rh1CG093800 Rh1DG100300 Rh1DG100400 Rh4AG058500 Rh4AG318800 Rh4AG422300 Rh4AG422400 Rh4BG057300 Rh4BG326900 Rh4BG327000 Rh4BG434300 Rh4BG434600 Rh4CG063100 Rh4CG342300 Rh4CG342400 Rh4CG449100 Rh4CG449200 Rh4CG449300 Rh4CG449500 Rh4DG054000 Rh4DG054100 Rh4DG322100 Rh4DG430300 Rh4DG430400 Rh4DG430800 Rh5AG446700 Rh5AG446800 Rh5BG167000 Rh5BG465000 Rh5CG182600 Rh5DG168900 Rh5DG169000 Rh5DG478700 Rh6AG251900 Rh6BG255300 Rh6CG254700 Rh6DG246500 Rh7AG374400 Rh7BG363700 Rh7CG392700 Rh7CG392800 Rh7DG374500 Rh7DG374600
rosa_wichuraiana Rw0G001550 Rw0G005020 Rw1G007510 Rw1G007530 Rw1G007570 Rw3G022750 Rw4G004710 Rw4G027720 Rw4G036230 Rw4G036240 Rw5G015340 Rw6G021880 Rw7G031610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 762
AccII CGCG 1 cut(s) 110
AciI CCGC 4 cut(s) 8, 45, 86, 122
AclWI GGATC 2 cut(s) 849, 934
AcoI YGGCCR 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 308, 689
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 344, 938
AfiI CCNNNNNNNGG 4 cut(s) 22, 28, 377, 772
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 7 cut(s) 115, 264, 335, 433, 521, 814, 950
AjnI CCWGG 3 cut(s) 385, 765, 909
AluBI AGCT 9 cut(s) 142, 167, 215, 268, 308, 598, 785, 795, 920
AluI AGCT 9 cut(s) 142, 167, 215, 268, 308, 598, 785, 795, 920
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 237, 597
AlwI GGATC 2 cut(s) 849, 934
AlwNI CAGNNNCTG 1 cut(s) 823
Ama87I CYCGRG 1 cut(s) 723
AoxI GGCC 7 cut(s) 49, 87, 132, 226, 368, 472, 615
ApeKI GCWGC 2 cut(s) 5, 446
AspS9I GGNCC 2 cut(s) 50, 400
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 4 cut(s) 136, 209, 278, 469
AvaI CYCGRG 1 cut(s) 723
AvaII GGWCC 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 677
BaeI ACNNNNGTAYC 2 cut(s) 326, 359
BanI GGYRCC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 17, 458
BccI CCATC 4 cut(s) 143, 750, 917, 956
BceAI ACGGC 2 cut(s) 99, 135
BciT130I CCWGG 3 cut(s) 387, 767, 911
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 2 cut(s) 237, 597
BfaI CTAG 4 cut(s) 143, 218, 507, 782
BfmI CTRYAG 1 cut(s) 148
BisI GCNGC 4 cut(s) 6, 9, 87, 447
BlsI GCNGC 4 cut(s) 7, 10, 88, 448
Bme1390I CCNGG 4 cut(s) 23, 387, 767, 911
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 723
BmgT120I GGNCC 2 cut(s) 50, 400
BmiI GGNNCC 6 cut(s) 40, 52, 181, 402, 664, 676
BmrFI CCNGG 4 cut(s) 23, 387, 767, 911
BmrI ACTGGG 1 cut(s) 847
BmsI GCATC 2 cut(s) 271, 885
BmuI ACTGGG 1 cut(s) 847
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 28, 377, 772
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 4 cut(s) 342, 371, 666, 842
BseBI CCWGG 3 cut(s) 387, 767, 911
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 967
BseLI CCNNNNNNNGG 4 cut(s) 22, 28, 377, 772
BseMII CTCAG 3 cut(s) 567, 590, 755
BseNI ACTGG 4 cut(s) 342, 371, 666, 842
BseRI GAGGAG 2 cut(s) 31, 636
BseSI GKGCMC 1 cut(s) 677
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 17, 458
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 7 cut(s) 51, 89, 134, 228, 370, 474, 617
BshNI GGYRCC 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 723
BsiSI CCGG 2 cut(s) 23, 48
BslI CCNNNNNNNGG 4 cut(s) 22, 28, 377, 772
BsmAI GTCTC 2 cut(s) 237, 597
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 7 cut(s) 51, 89, 134, 228, 370, 474, 617
BsoBI CYCGRG 1 cut(s) 723
Bsp1286I GDGCHC 2 cut(s) 36, 677
Bsp143I GATC 5 cut(s) 292, 841, 856, 867, 926
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 4 cut(s) 8, 45, 86, 122
BspANI GGCC 7 cut(s) 51, 89, 134, 228, 370, 474, 617
BspCNI CTCAG 3 cut(s) 566, 591, 754
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 6 cut(s) 40, 52, 181, 402, 664, 676
BspPI GGATC 2 cut(s) 849, 934
BspQI GCTCTTC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 4 cut(s) 342, 371, 666, 842
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 5 cut(s) 292, 841, 856, 867, 926
BssNAI GTATAC 1 cut(s) 763
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 450
Bst1107I GTATAC 1 cut(s) 763
Bst2UI CCWGG 3 cut(s) 387, 767, 911
Bst6I CTCTTC 1 cut(s) 785
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 823
BstC8I GCNNGC 4 cut(s) 13, 49, 62, 383
BstDEI CTNAG 3 cut(s) 553, 599, 741
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 1 cut(s) 967
BstFNI CGCG 1 cut(s) 110
BstKTI GATC 5 cut(s) 295, 844, 859, 870, 929
BstMAI GTCTC 2 cut(s) 237, 597
BstMBI GATC 5 cut(s) 292, 841, 856, 867, 926
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 3 cut(s) 57, 782, 823
BstNI CCWGG 3 cut(s) 387, 767, 911
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 4 cut(s) 21, 385, 765, 909
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 17, 458
BstX2I RGATCY 1 cut(s) 841
BstYI RGATCY 1 cut(s) 841
BstZ17I GTATAC 1 cut(s) 763
BstZI CGGCCG 1 cut(s) 87
BsuRI GGCC 7 cut(s) 51, 89, 134, 228, 370, 474, 617
BtgI CCRYGG 1 cut(s) 450
BtsCI GGATG 1 cut(s) 967
BtsIMutI CAGTG 2 cut(s) 364, 382
Cac8I GCNNGC 4 cut(s) 13, 49, 62, 383
CaiI CAGNNNCTG 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 2 cut(s) 50, 400
CseI GACGC 2 cut(s) 116, 209
CsiI ACCWGGT 2 cut(s) 765, 909
Csp6I GTAC 2 cut(s) 343, 937
CviAII CATG 3 cut(s) 61, 451, 683
CviQI GTAC 2 cut(s) 343, 937
DdeI CTNAG 3 cut(s) 553, 599, 741
DpnI GATC 5 cut(s) 294, 843, 858, 869, 928
DpnII GATC 5 cut(s) 292, 841, 856, 867, 926
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
EciI GGCGGA 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 400
Eco52I CGGCCG 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 308, 689
Eco88I CYCGRG 1 cut(s) 723
EcoRII CCWGG 3 cut(s) 385, 765, 909
EcoT14I CCWWGG 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 3 cut(s) 64, 454, 686
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FatI CATG 3 cut(s) 60, 450, 682
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 4 cut(s) 6, 9, 87, 447
Fsp4HI GCNGC 4 cut(s) 6, 9, 87, 447
FspBI CTAG 4 cut(s) 143, 218, 507, 782
GluI GCNGC 4 cut(s) 6, 9, 87, 447
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 7 cut(s) 51, 89, 134, 228, 370, 474, 617
HapII CCGG 2 cut(s) 23, 48
HgaI GACGC 2 cut(s) 116, 209
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 3 cut(s) 64, 454, 686
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 8 cut(s) 190, 236, 328, 361, 458, 492, 574, 820
HpaII CCGG 2 cut(s) 23, 48
HphI GGTGA 4 cut(s) 136, 209, 278, 469
Hpy166II GTNNAC 3 cut(s) 400, 412, 763
Hpy188I TCNGA 4 cut(s) 184, 327, 360, 850
Hpy188III TCNNGA 5 cut(s) 218, 240, 313, 568, 707
Hpy8I GTNNAC 3 cut(s) 400, 412, 763
HpyAV CCTTC 2 cut(s) 85, 311
HpyCH4V TGCA 4 cut(s) 612, 638, 656, 750
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 782, 823
HpyF3I CTNAG 3 cut(s) 553, 599, 741
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 3 cut(s) 64, 454, 686
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Kzo9I GATC 5 cut(s) 292, 841, 856, 867, 926
LguI GCTCTTC 1 cut(s) 785
LmnI GCTCC 2 cut(s) 44, 185
Lsp1109I GCAGC 2 cut(s) 17, 458
LweI GCATC 2 cut(s) 271, 885
MabI ACCWGGT 2 cut(s) 765, 909
MaeI CTAG 4 cut(s) 143, 218, 507, 782
MaeIII GTNAC 1 cut(s) 197
MalI GATC 5 cut(s) 294, 843, 858, 869, 928
MboI GATC 5 cut(s) 292, 841, 856, 867, 926
MboII GAAGA 8 cut(s) 323, 467, 604, 802, 863, 898, 901, 962
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 2 cut(s) 36, 677
MluCI AATT 3 cut(s) 392, 657, 953
MluI ACGCGT 1 cut(s) 108
MlyI GAGTC 3 cut(s) 184, 501, 583
MnlI CCTC 9 cut(s) 52, 80, 124, 485, 558, 562, 657, 712, 801
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 3 cut(s) 207, 900, 973
MspA1I CMGCKG 2 cut(s) 8, 920
MspI CCGG 2 cut(s) 23, 48
MspR9I CCNGG 4 cut(s) 23, 387, 767, 911
MunI CAATTG 1 cut(s) 657
Mva1269I GAATGC 1 cut(s) 638
MvaI CCWGG 3 cut(s) 387, 767, 911
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 57, 782, 823
NaeI GCCGGC 1 cut(s) 49
NciI CCSGG 1 cut(s) 23
NcoI CCATGG 1 cut(s) 450
NdeII GATC 5 cut(s) 292, 841, 856, 867, 926
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 3 cut(s) 64, 454, 686
NlaIV GGNNCC 6 cut(s) 40, 52, 181, 402, 664, 676
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 1 cut(s) 64
PaeI GCATGC 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 785
PctI GAATGC 1 cut(s) 638
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 5 cut(s) 236, 328, 361, 458, 820
PfoI TCCNGGA 1 cut(s) 21
PkrI GCNGC 4 cut(s) 7, 10, 88, 448
PleI GAGTC 3 cut(s) 184, 500, 582
PpsI GAGTC 3 cut(s) 184, 500, 582
Psp6I CCWGG 3 cut(s) 385, 765, 909
PspGI CCWGG 3 cut(s) 385, 765, 909
PspN4I GGNNCC 6 cut(s) 40, 52, 181, 402, 664, 676
PspPI GGNCC 2 cut(s) 50, 400
PstNI CAGNNNCTG 1 cut(s) 823
PsuI RGATCY 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 2 cut(s) 344, 938
RsaNI GTAC 2 cut(s) 343, 937
SapI GCTCTTC 1 cut(s) 785
SaqAI TTAA 3 cut(s) 207, 900, 973
SatI GCNGC 4 cut(s) 6, 9, 87, 447
Sau3AI GATC 5 cut(s) 292, 841, 856, 867, 926
Sau96I GGNCC 2 cut(s) 50, 400
SchI GAGTC 3 cut(s) 184, 501, 583
ScrFI CCNGG 4 cut(s) 23, 387, 767, 911
SduI GDGCHC 2 cut(s) 36, 677
SexAI ACCWGGT 2 cut(s) 765, 909
SfaNI GCATC 2 cut(s) 271, 885
SfcI CTRYAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SphI GCATGC 1 cut(s) 64
Sse9I AATT 3 cut(s) 392, 657, 953
SsiI CCGC 4 cut(s) 8, 45, 86, 122
SspMI CTAG 4 cut(s) 143, 218, 507, 782
StyD4I CCNGG 4 cut(s) 21, 385, 765, 909
StyI CCWWGG 1 cut(s) 450
TaqI TCGA 1 cut(s) 567
TasI AATT 3 cut(s) 392, 657, 953
TauI GCSGC 2 cut(s) 11, 89
TfiI GAWTC 5 cut(s) 236, 328, 361, 458, 820
Tru1I TTAA 3 cut(s) 207, 900, 973
Tru9I TTAA 3 cut(s) 207, 900, 973
TscAI CASTG 2 cut(s) 371, 382
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 2 cut(s) 5, 446
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 371, 382
VpaK11BI GGWCC 1 cut(s) 400
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 762
XspI CTAG 4 cut(s) 143, 218, 507, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.