Rmu_ssc0000027.1_g000010

Non-functional NADPH-dependent codeinone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000027.1
OG0000205
Physical Location & Seq
Forward (+)
38607 .. 41248
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000027.1_g000010.1.cds

Sequence Viewer

Length: 918 bp
atgacctcgagtttatccactcgatccacctccgtcgtcgtccccactaagattagccacctctcctcctcctcatccttcaccactcccaccaataagccccgccagaactgcagtttcccaaccgaaaaaggcgctgtgacgccactcaatcggccgccggagctcgacctggagcttctgtccctcaaaagctcgagtcctacacctcactcaaggacctcctcctctcctcctcctccttcgtcgctgtccagtccccgatgcccaacttatccaccaactccggctactagatatccatccgtagccgcctcgtcaagccggtcgcctgggcctatctccagtccgtgtcctccacttgcggcccacacttgctccgccgcctatggctcaagttcaccccctgcctctccttcatcacccattgccatcattttggaaatttcatttagagagagagagagagagagagagagagagagagagaagggcagagacagttcgccggagcattgccacggttttgagccatggttgatcgagaatagggtgatgagtctccacgaagaagaaggtttgggtgcccagagaaaattctgggtacctgcgtgttggcgtgtggaccattttccggtgtcgctggatgatgcgaaggcaggaccagactcgtcggaggccggtgagtacagtagtggtggaggtgagctgcatggtagtgtgtgcattagatgggtttatcaggtaggagcaactcttgcagtgaagagctacaacaaggagaggttgaagcagaatgtgcaggtgttcgactgggaactaacagaagaggatcttgagaagatcaatcaaatcccacggcgcaaaatgatagttcgagaagaggagttggttacggctaccggaccatacaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

305

Amino Acids

33.44

Weight (kDa)

7.08

Isoelectric Point (pI)

70.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59950 AT1G59960
fragaria_vesca FvH4_2g01280 FvH4_3g20300 FvH4_3g20300 FvH4_4g25591 FvH4_4g25600 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25612 FvH4_4g25612 FvH4_4g25620 FvH4_4g35840
malus_domestica MD04G1063200.v1.1 MD10G1055100.v1.1 MD13G1014700.v1.1 MD13G1101200.v1.1 MD16G1101500.v1.1
prunus_persica Prupe.1G244700_v2.0.a1 Prupe.1G245100_v2.0.a1 Prupe.1G245400_v2.0.a1 Prupe.1G338900_v2.0.a1 Prupe.5G239400_v2.0.a1
pyrus_communis pycom13g08830 pycom16g01150 pycom16g08620
rosa_chinensis RchiOBHm_Chr1g0329311 RchiOBHm_Chr3g0483001 RchiOBHm_Chr4g0395321 RchiOBHm_Chr4g0433021 RchiOBHm_Chr4g0445371 RchiOBHm_Chr4g0445381 RchiOBHm_Chr4g0445421 RchiOBHm_Chr5g0023771 RchiOBHm_Chr5g0023781 RchiOBHm_Chr5g0068101 RchiOBHm_Chr5g0068111 RchiOBHm_Chr6g0281251 RchiOBHm_Chr7g0225361
rosa_laevigata RLG00000001827 RLG00000005742 RLG00000005743 RLG00000005744 RLG00000006791 RLG00000009629 RLG00000012981 RLG00000023308 RLG00000029902 RLG00000032738 RLG00000032739 RLG00000035956 RLG00000035957
rosa_multiflora Rmu_co7982306.1_g000001 Rmu_co8108716.1_g000001 Rmu_co8139310.1_g000001 Rmu_co8479199.1_g000001 Rmu_sc0000050.1_g000005 Rmu_sc0000050.1_g000006 Rmu_sc0000847.1_g000048 Rmu_sc0001372.1_g000008 Rmu_sc0001762.1_g000035 Rmu_sc0002791.1_g000001 Rmu_sc0002791.1_g000002 Rmu_sc0002791.1_g000005 Rmu_sc0003252.1_g000001 Rmu_sc0003351.1_g000013 Rmu_sc0006184.1_g000008 Rmu_sc0009518.1_g000006 Rmu_sc0010817.1_g000030 Rmu_sc0016867.1_g000002 Rmu_ssc0000027.1_g000010
rosa_roxburghii Rroxscaffold_1G00012480 Rroxscaffold_1G00012490 Rroxscaffold_1G00013240 Rroxscaffold_1G00013250 Rroxscaffold_1G00055410 Rroxscaffold_1G00055420 Rroxscaffold_3G00233890 Rroxscaffold_4G00321220 Rroxscaffold_5G00340230 Rroxscaffold_5G00340260 Rroxscaffold_6G00398750 Rroxscaffold_7G00187200
rosa_rugosa Rorug01G0078500 Rorug03G0202900 Rorug03G0358000 Rorug04G0361100 Rorug04G0361200 Rorug05G0079600 Rorug06G0140100 Rorug07G0228800
rosa_samantha Rh1AG096900 Rh1BG077500 Rh1CG093800 Rh1DG100300 Rh1DG100400 Rh4AG058500 Rh4AG318800 Rh4AG422300 Rh4AG422400 Rh4BG057300 Rh4BG326900 Rh4BG327000 Rh4BG434300 Rh4BG434600 Rh4CG063100 Rh4CG342300 Rh4CG342400 Rh4CG449100 Rh4CG449200 Rh4CG449300 Rh4CG449500 Rh4DG054000 Rh4DG054100 Rh4DG322100 Rh4DG430300 Rh4DG430400 Rh4DG430800 Rh5AG446700 Rh5AG446800 Rh5BG167000 Rh5BG465000 Rh5CG182600 Rh5DG168900 Rh5DG169000 Rh5DG478700 Rh6AG251900 Rh6BG255300 Rh6CG254700 Rh6DG246500 Rh7AG374400 Rh7BG363700 Rh7CG392700 Rh7CG392800 Rh7DG374500 Rh7DG374600
rosa_wichuraiana Rw0G001550 Rw0G005020 Rw1G007510 Rw1G007530 Rw1G007570 Rw3G022750 Rw4G004710 Rw4G027720 Rw4G036230 Rw4G036240 Rw5G015340 Rw6G021880 Rw7G031610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 793
Acc36I ACCTGC 2 cut(s) 616, 793
Acc65I GGTACC 1 cut(s) 604
AccB1I GGYRCC 2 cut(s) 584, 604
AciI CCGC 6 cut(s) 103, 158, 312, 365, 381, 384
AclWI GGATC 2 cut(s) 18, 840
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 2 cut(s) 444, 596
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 2 cut(s) 606, 689
AfiI CCNNNNNNNGG 1 cut(s) 634
AgsI TTSAA 1 cut(s) 790
AjnI CCWGG 2 cut(s) 171, 331
AluBI AGCT 5 cut(s) 166, 178, 195, 709, 771
AluI AGCT 5 cut(s) 166, 178, 195, 709, 771
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 2 cut(s) 492, 566
AlwI GGATC 2 cut(s) 18, 840
Ama87I CYCGRG 2 cut(s) 7, 196
AoxI GGCC 4 cut(s) 155, 335, 366, 678
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 2 cut(s) 444, 596
Asp718I GGTACC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 137, 864
AspS9I GGNCC 6 cut(s) 219, 335, 367, 625, 662, 905
AsuHPI GGTGA 6 cut(s) 73, 393, 414, 565, 695, 716
AvaI CYCGRG 2 cut(s) 7, 196
AvaII GGWCC 4 cut(s) 219, 625, 662, 905
BaeGI GKGCMC 1 cut(s) 589
BaeI ACNNNNGTAYC 2 cut(s) 596, 629
BanI GGYRCC 2 cut(s) 584, 604
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 696
BccI CCATC 3 cut(s) 310, 440, 726
BceAI ACGGC 2 cut(s) 875, 912
BciT130I CCWGG 2 cut(s) 173, 333
BcoDI GTCTC 2 cut(s) 492, 566
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 138
BfuAI ACCTGC 2 cut(s) 616, 793
BisI GCNGC 5 cut(s) 158, 312, 366, 384, 710
BlsI GCNGC 5 cut(s) 159, 313, 367, 385, 711
Bme1390I CCNGG 2 cut(s) 173, 333
Bme18I GGWCC 4 cut(s) 219, 625, 662, 905
BmeT110I CYCGRG 2 cut(s) 7, 196
BmgT120I GGNCC 6 cut(s) 219, 335, 367, 625, 662, 905
BmiI GGNNCC 2 cut(s) 586, 606
BmrFI CCNGG 2 cut(s) 173, 333
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 2 cut(s) 254, 640
BmuI ACTGGG 1 cut(s) 823
BpmI CTGGAG 2 cut(s) 194, 328
BpuEI CTTGAG 3 cut(s) 199, 379, 857
BsaBI GATNNNNATC 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 4 cut(s) 332, 520, 533, 857
BsaWI WCCGGW 2 cut(s) 634, 902
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 167, 197, 362, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse118I RCCGGY 2 cut(s) 324, 680
Bse1I ACTGG 3 cut(s) 255, 345, 818
Bse3DI GCAATG 2 cut(s) 426, 514
Bse8I GATNNNNATC 1 cut(s) 301
BseBI CCWGG 2 cut(s) 173, 333
BseDI CCNNGG 4 cut(s) 332, 520, 533, 857
BseGI GGATG 3 cut(s) 74, 302, 652
BseJI GATNNNNATC 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 2 cut(s) 426, 514
BseNI ACTGG 3 cut(s) 255, 345, 818
BseRI GAGGAG 9 cut(s) 55, 58, 61, 214, 217, 222, 225, 228, 899
BseSI GKGCMC 1 cut(s) 589
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 696
BsgI GTGCAG 1 cut(s) 821
Bsh1285I CGRYCG 2 cut(s) 158, 329
BshFI GGCC 4 cut(s) 157, 337, 368, 680
BshNI GGYRCC 2 cut(s) 584, 604
BsiEI CGRYCG 2 cut(s) 158, 329
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 7, 196
BsiSI CCGG 7 cut(s) 161, 287, 325, 509, 635, 681, 903
BslFI GGGAC 3 cut(s) 26, 169, 243
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 2 cut(s) 492, 566
BsmFI GGGAC 3 cut(s) 26, 169, 243
BsnI GGCC 4 cut(s) 157, 337, 368, 680
BsoBI CYCGRG 2 cut(s) 7, 196
Bsp1286I GDGCHC 2 cut(s) 168, 589
Bsp143I GATC 4 cut(s) 23, 540, 832, 843
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 6 cut(s) 103, 158, 312, 365, 381, 384
BspANI GGCC 4 cut(s) 157, 337, 368, 680
BspLI GGNNCC 2 cut(s) 586, 606
BspMAI CTGCAG 1 cut(s) 116
BspMI ACCTGC 2 cut(s) 616, 793
BspPI GGATC 2 cut(s) 18, 840
BspQI GCTCTTC 1 cut(s) 761
BspT107I GGYRCC 2 cut(s) 584, 604
BsrDI GCAATG 2 cut(s) 426, 514
BsrFI RCCGGY 2 cut(s) 324, 680
BsrI ACTGG 3 cut(s) 255, 345, 818
BssAI RCCGGY 2 cut(s) 324, 680
BssECI CCNNGG 4 cut(s) 332, 520, 533, 857
BssMI GATC 4 cut(s) 23, 540, 832, 843
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 533
Bst2UI CCWGG 2 cut(s) 173, 333
Bst4CI ACNGT 3 cut(s) 503, 524, 692
Bst6I CTCTTC 3 cut(s) 761, 822, 876
BstACI GRCGYC 1 cut(s) 143
BstAPI GCANNNNNTGC 2 cut(s) 758, 799
BstDEI CTNAG 1 cut(s) 48
BstDSI CCRYGG 3 cut(s) 520, 533, 857
BstF5I GGATG 3 cut(s) 74, 302, 652
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 864
BstKTI GATC 4 cut(s) 26, 543, 835, 846
BstMAI GTCTC 2 cut(s) 492, 566
BstMBI GATC 4 cut(s) 23, 540, 832, 843
BstMCI CGRYCG 2 cut(s) 158, 329
BstMWI GCNNNNNNNGC 4 cut(s) 111, 163, 758, 799
BstNI CCWGG 2 cut(s) 173, 333
BstSCI CCNGG 2 cut(s) 171, 331
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 832
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 832
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 4 cut(s) 157, 337, 368, 680
BtgI CCRYGG 3 cut(s) 520, 533, 857
BtsCI GGATG 3 cut(s) 74, 302, 652
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
BveI ACCTGC 2 cut(s) 616, 793
CfoI GCGC 2 cut(s) 137, 864
Cfr10I RCCGGY 2 cut(s) 324, 680
Cfr13I GGNCC 6 cut(s) 219, 335, 367, 625, 662, 905
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 605, 688
CviAII CATG 2 cut(s) 534, 713
CviQI GTAC 2 cut(s) 605, 688
DdeI CTNAG 1 cut(s) 48
DpnI GATC 4 cut(s) 25, 542, 834, 845
DpnII GATC 4 cut(s) 23, 540, 832, 843
EaeI YGGCCR 1 cut(s) 155
EagI CGGCCG 1 cut(s) 155
Eam1104I CTCTTC 3 cut(s) 761, 822, 876
EarI CTCTTC 3 cut(s) 761, 822, 876
EciI GGCGGA 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 299
Eco47I GGWCC 4 cut(s) 219, 625, 662, 905
Eco52I CGGCCG 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 7, 196
EcoICRI GAGCTC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 171, 331
EcoRV GATATC 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 537, 716
FaiI YATR 4 cut(s) 390, 535, 714, 910
FalI AAGNNNNNCTT 2 cut(s) 819, 851
FaqI GGGAC 3 cut(s) 26, 169, 243
FatI CATG 2 cut(s) 533, 712
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 5 cut(s) 158, 312, 366, 384, 710
FokI GGATG 3 cut(s) 61, 289, 659
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 5 cut(s) 158, 312, 366, 384, 710
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 136, 863
GluI GCNGC 5 cut(s) 158, 312, 366, 384, 710
GsuI CTGGAG 2 cut(s) 194, 328
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 4 cut(s) 157, 337, 368, 680
HapII CCGG 7 cut(s) 161, 287, 325, 509, 635, 681, 903
HgaI GACGC 1 cut(s) 151
HhaI GCGC 2 cut(s) 137, 864
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 2 cut(s) 537, 716
Hin6I GCGC 2 cut(s) 135, 862
HinP1I GCGC 2 cut(s) 135, 862
HinfI GANTC 3 cut(s) 199, 559, 668
HpaII CCGG 7 cut(s) 161, 287, 325, 509, 635, 681, 903
HphI GGTGA 6 cut(s) 73, 393, 414, 565, 695, 716
Hpy166II GTNNAC 2 cut(s) 401, 625
Hpy188I TCNGA 1 cut(s) 676
Hpy188III TCNNGA 3 cut(s) 544, 836, 878
Hpy8I GTNNAC 2 cut(s) 401, 625
Hpy99I CGWCG 4 cut(s) 38, 41, 250, 676
HpyAV CCTTC 6 cut(s) 88, 252, 426, 484, 569, 649
HpyCH4III ACNGT 3 cut(s) 503, 524, 692
HpyCH4V TGCA 5 cut(s) 114, 712, 726, 761, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 163, 758, 799
HpyF3I CTNAG 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 2 cut(s) 537, 716
HspAI GCGC 2 cut(s) 135, 862
KpnI GGTACC 1 cut(s) 608
Kzo9I GATC 4 cut(s) 23, 540, 832, 843
LguI GCTCTTC 1 cut(s) 761
LmnI GCTCC 5 cut(s) 163, 175, 383, 511, 749
Lsp1109I GCAGC 1 cut(s) 696
LweI GCATC 2 cut(s) 254, 640
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 139, 892
MalI GATC 4 cut(s) 25, 542, 834, 845
MboI GATC 4 cut(s) 23, 540, 832, 843
MboII GAAGA 6 cut(s) 581, 584, 778, 839, 853, 893
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 2 cut(s) 168, 589
MluCI AATT 2 cut(s) 444, 596
MlyI GAGTC 3 cut(s) 208, 568, 662
MmeI TCCRAC 1 cut(s) 654
MslI CAYNNNNRTG 1 cut(s) 717
MspI CCGG 7 cut(s) 161, 287, 325, 509, 635, 681, 903
MspR9I CCNGG 2 cut(s) 173, 333
MvaI CCWGG 2 cut(s) 173, 333
MwoI GCNNNNNNNGC 4 cut(s) 111, 163, 758, 799
NcoI CCATGG 1 cut(s) 533
NdeII GATC 4 cut(s) 23, 540, 832, 843
NlaIII CATG 2 cut(s) 537, 716
NlaIV GGNNCC 2 cut(s) 586, 606
NmuCI GTSAC 1 cut(s) 139
PaeR7I CTCGAG 2 cut(s) 7, 196
PaqCI CACCTGC 1 cut(s) 793
PciSI GCTCTTC 1 cut(s) 761
PkrI GCNGC 5 cut(s) 159, 313, 367, 385, 711
PleI GAGTC 3 cut(s) 207, 567, 662
PpsI GAGTC 3 cut(s) 207, 567, 662
PpuMI RGGWCCY 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 171, 331
PspGI CCWGG 2 cut(s) 171, 331
PspN4I GGNNCC 2 cut(s) 586, 606
PspPI GGNCC 6 cut(s) 219, 335, 367, 625, 662, 905
PspPPI RGGWCCY 1 cut(s) 219
PspXI VCTCGAGB 2 cut(s) 7, 196
PstI CTGCAG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 606, 689
RsaNI GTAC 2 cut(s) 605, 688
RseI CAYNNNNRTG 1 cut(s) 717
SacI GAGCTC 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 761
SatI GCNGC 5 cut(s) 158, 312, 366, 384, 710
Sau3AI GATC 4 cut(s) 23, 540, 832, 843
Sau96I GGNCC 6 cut(s) 219, 335, 367, 625, 662, 905
SchI GAGTC 3 cut(s) 208, 568, 662
ScrFI CCNGG 2 cut(s) 173, 333
SduI GDGCHC 2 cut(s) 168, 589
SfaNI GCATC 2 cut(s) 254, 640
SfcI CTRYAG 1 cut(s) 112
Sfr274I CTCGAG 2 cut(s) 7, 196
SinI GGWCC 4 cut(s) 219, 625, 662, 905
SlaI CTCGAG 2 cut(s) 7, 196
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 5 cut(s) 7, 196, 214, 394, 836
SmoI CTYRAG 5 cut(s) 7, 196, 214, 394, 836
Sse9I AATT 2 cut(s) 444, 596
SsiI CCGC 6 cut(s) 103, 158, 312, 365, 381, 384
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 171, 331
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 503, 524, 692
TaqI TCGA 7 cut(s) 8, 22, 168, 197, 543, 810, 877
TasI AATT 2 cut(s) 444, 596
TatI WGTACW 1 cut(s) 687
TauI GCSGC 4 cut(s) 160, 314, 368, 386
TscAI CASTG 1 cut(s) 768
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 709
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 408, 438
TspGWI ACGGA 3 cut(s) 22, 295, 339
TspRI CASTG 1 cut(s) 768
VpaK11BI GGWCC 4 cut(s) 219, 625, 662, 905
XapI RAATTY 2 cut(s) 444, 596
XhoI CTCGAG 2 cut(s) 7, 196
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.