Rw4G004710

Non-functional NADPH-dependent codeinone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000205
Physical Location & Seq
Forward (+)
9241900 .. 9242817
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004710.1

Sequence Viewer

Length: 918 bp
ATGGCAGCAACTCATATCCCAGAGGTGGTTCTTGAATCCTCCAATGGCCGCAGGACCATGCCTGTGCTTGGCTTCGGCACAGCATCCAACAATTTACAACCAGAGGTTTTCATAGAAGCTGTTCTCGAGGCCATAAAGCTCGGTTACCGCCACTTCGACACAGCTTCCCTGTACGGCTCGGAGCAGAGTCTGGGAGTAGCCATAGCCCAAGCACTCAAACTCGGCCTCGTGGCTTCTCGAGACGAGCTCTTCATCACTTCCAAGCTTTGGTCTAATGATGCTCACCCTGATTTGGTTCTTCCTGCTCTAAAGAAATCCCTTCAGAACCTTGAATTGGATTACCTTGATCTGTATCTGATTCACTGGCCCATCAGTGCGACGCCTGGGAAATTGAGTCACTTTCTCGAGGAGAAGGACCAAATGCCGATGGACTTCAAGGGCGTGTGGGTAGCCATGGAAGAAGCTCAGAGACTTGGCCTCACCAAATCCATTGGAATCAGCAATTTCTCTACCAAAAAGACTCAGAATTTGCTCTCTTTTGCTACTATTCCTCCTTCAGTCAATCAAGTTGAGATGAGCCCATTTTGGCAACAAAAGAAGCTCAGAGAATTCTGCAAAGCCAATGGTATAGTTGTGACTGCCTTCTCCCCATTGGGTGCCATAGGAACCAGTTGGGGTACCAATTATGTTCTCGAAAGCAAAGTGCTTAATGAAATCGCTAAGGCTCGGGGAAAGACTGTTGCTCAGGTCTGCATTAGATGGGTTTATCAGGTAGGAGCAACTCTTGCAGTGAAGAGCTACAACAAGGAGAGGTTGAAGCAGAATGTGCAGGTGTTCGACTGGGAACTAACAGAAGAGGATCTTGAGAAGATCAATCAAATCCCACAGCGCAGAATGATATCAAAAAAAAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

305

Amino Acids

34.18

Weight (kDa)

8.87

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 294 8.8e-57 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59950 AT1G59960
fragaria_vesca FvH4_2g01280 FvH4_3g20300 FvH4_3g20300 FvH4_4g25591 FvH4_4g25600 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25612 FvH4_4g25612 FvH4_4g25620 FvH4_4g35840
malus_domestica MD04G1063200.v1.1 MD10G1055100.v1.1 MD13G1014700.v1.1 MD13G1101200.v1.1 MD16G1101500.v1.1
prunus_persica Prupe.1G244700_v2.0.a1 Prupe.1G245100_v2.0.a1 Prupe.1G245400_v2.0.a1 Prupe.1G338900_v2.0.a1 Prupe.5G239400_v2.0.a1
pyrus_communis pycom13g08830 pycom16g01150 pycom16g08620
rosa_chinensis RchiOBHm_Chr1g0329311 RchiOBHm_Chr3g0483001 RchiOBHm_Chr4g0395321 RchiOBHm_Chr4g0433021 RchiOBHm_Chr4g0445371 RchiOBHm_Chr4g0445381 RchiOBHm_Chr4g0445421 RchiOBHm_Chr5g0023771 RchiOBHm_Chr5g0023781 RchiOBHm_Chr5g0068101 RchiOBHm_Chr5g0068111 RchiOBHm_Chr6g0281251 RchiOBHm_Chr7g0225361
rosa_laevigata RLG00000001827 RLG00000005742 RLG00000005743 RLG00000005744 RLG00000006791 RLG00000009629 RLG00000012981 RLG00000023308 RLG00000029902 RLG00000032738 RLG00000032739 RLG00000035956 RLG00000035957
rosa_multiflora Rmu_co7982306.1_g000001 Rmu_co8108716.1_g000001 Rmu_co8139310.1_g000001 Rmu_co8479199.1_g000001 Rmu_sc0000050.1_g000005 Rmu_sc0000050.1_g000006 Rmu_sc0000847.1_g000048 Rmu_sc0001372.1_g000008 Rmu_sc0001762.1_g000035 Rmu_sc0002791.1_g000001 Rmu_sc0002791.1_g000002 Rmu_sc0002791.1_g000005 Rmu_sc0003252.1_g000001 Rmu_sc0003351.1_g000013 Rmu_sc0006184.1_g000008 Rmu_sc0009518.1_g000006 Rmu_sc0010817.1_g000030 Rmu_sc0016867.1_g000002 Rmu_ssc0000027.1_g000010
rosa_roxburghii Rroxscaffold_1G00012480 Rroxscaffold_1G00012490 Rroxscaffold_1G00013240 Rroxscaffold_1G00013250 Rroxscaffold_1G00055410 Rroxscaffold_1G00055420 Rroxscaffold_3G00233890 Rroxscaffold_4G00321220 Rroxscaffold_5G00340230 Rroxscaffold_5G00340260 Rroxscaffold_6G00398750 Rroxscaffold_7G00187200
rosa_rugosa Rorug01G0078500 Rorug03G0202900 Rorug03G0358000 Rorug04G0361100 Rorug04G0361200 Rorug05G0079600 Rorug06G0140100 Rorug07G0228800
rosa_samantha Rh1AG096900 Rh1BG077500 Rh1CG093800 Rh1DG100300 Rh1DG100400 Rh4AG058500 Rh4AG318800 Rh4AG422300 Rh4AG422400 Rh4BG057300 Rh4BG326900 Rh4BG327000 Rh4BG434300 Rh4BG434600 Rh4CG063100 Rh4CG342300 Rh4CG342400 Rh4CG449100 Rh4CG449200 Rh4CG449300 Rh4CG449500 Rh4DG054000 Rh4DG054100 Rh4DG322100 Rh4DG430300 Rh4DG430400 Rh4DG430800 Rh5AG446700 Rh5AG446800 Rh5BG167000 Rh5BG465000 Rh5CG182600 Rh5DG168900 Rh5DG169000 Rh5DG478700 Rh6AG251900 Rh6BG255300 Rh6CG254700 Rh6DG246500 Rh7AG374400 Rh7BG363700 Rh7CG392700 Rh7CG392800 Rh7DG374500 Rh7DG374600
rosa_wichuraiana Rw0G001550 Rw0G005020 Rw1G007510 Rw1G007530 Rw1G007570 Rw3G022750 Rw4G004710 Rw4G027720 Rw4G036230 Rw4G036240 Rw5G015340 Rw6G021880 Rw7G031610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 820
Acc36I ACCTGC 1 cut(s) 820
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 656, 677
AccB7I CCANNNNNTGG 1 cut(s) 267
AciI CCGC 2 cut(s) 49, 148
AclWI GGATC 1 cut(s) 867
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 526, 608
AcuI CTGAAG 2 cut(s) 305, 540
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 173, 679
AfiI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 334
AgsI TTSAA 4 cut(s) 35, 332, 436, 817
AjnI CCWGG 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 8 cut(s) 119, 139, 164, 247, 265, 464, 601, 798
AluI AGCT 8 cut(s) 119, 139, 164, 247, 265, 464, 601, 798
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 234, 463
AlwI GGATC 1 cut(s) 867
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 4 cut(s) 125, 237, 404, 726
AoxI GGCC 5 cut(s) 46, 129, 223, 365, 475
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 526, 608
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
Asp700I GAANNNNTTC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 54, 366, 415
AsuHPI GGTGA 2 cut(s) 275, 472
AvaI CYCGRG 4 cut(s) 125, 237, 404, 726
AvaII GGWCC 2 cut(s) 54, 415
BanI GGYRCC 2 cut(s) 656, 677
BanII GRGCYC 2 cut(s) 249, 581
BauI CACGAG 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 377, 421, 753
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 234, 463
BfuAI ACCTGC 1 cut(s) 820
BisI GCNGC 2 cut(s) 6, 49
BlsI GCNGC 2 cut(s) 7, 50
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 2 cut(s) 54, 415
BmeT110I CYCGRG 4 cut(s) 125, 237, 404, 726
BmgT120I GGNCC 3 cut(s) 54, 366, 415
BmiI GGNNCC 3 cut(s) 658, 667, 679
BmrFI CCNGG 1 cut(s) 384
BmrI ACTGGG 1 cut(s) 850
BmsI GCATC 2 cut(s) 92, 268
BmuI ACTGGG 1 cut(s) 850
BplI GAGNNNNNCTC 2 cut(s) 231, 263
Bpu10I CCTNAGC 2 cut(s) 720, 744
BpuEI CTTGAG 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 383, 453
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 334
Bse1I ACTGG 3 cut(s) 368, 669, 845
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 1 cut(s) 384
BseDI CCNNGG 2 cut(s) 383, 453
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 334
BseMII CTCAG 4 cut(s) 479, 536, 616, 758
BseNI ACTGG 3 cut(s) 368, 669, 845
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 848
BshFI GGCC 5 cut(s) 48, 131, 225, 367, 477
BshNI GGYRCC 2 cut(s) 656, 677
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 4 cut(s) 125, 237, 404, 726
BslI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 334
BsmAI GTCTC 2 cut(s) 234, 463
BsmBI CGTCTC 1 cut(s) 234
BsnI GGCC 5 cut(s) 48, 131, 225, 367, 477
BsoBI CYCGRG 4 cut(s) 125, 237, 404, 726
Bsp1286I GDGCHC 2 cut(s) 249, 581
Bsp143I GATC 3 cut(s) 346, 859, 870
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 2 cut(s) 49, 148
BspANI GGCC 5 cut(s) 48, 131, 225, 367, 477
BspCNI CTCAG 4 cut(s) 478, 535, 615, 757
BspLI GGNNCC 3 cut(s) 658, 667, 679
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 1 cut(s) 867
BspQI GCTCTTC 2 cut(s) 254, 788
BspT107I GGYRCC 2 cut(s) 656, 677
BsrI ACTGG 3 cut(s) 368, 669, 845
BssECI CCNNGG 2 cut(s) 383, 453
BssMI GATC 3 cut(s) 346, 859, 870
BssNI GRCGYC 1 cut(s) 380
BssSI CACGAG 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 3 cut(s) 254, 788, 849
BstACI GRCGYC 1 cut(s) 380
BstAPI GCANNNNNTGC 2 cut(s) 785, 826
BstDEI CTNAG 5 cut(s) 465, 522, 602, 720, 744
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 3 cut(s) 349, 862, 873
BstMAI GTCTC 2 cut(s) 234, 463
BstMBI GATC 3 cut(s) 346, 859, 870
BstMWI GCNNNNNNNGC 2 cut(s) 785, 826
BstNI CCWGG 1 cut(s) 384
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
BsuRI GGCC 5 cut(s) 48, 131, 225, 367, 477
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 3 cut(s) 361, 379, 795
BveI ACCTGC 1 cut(s) 820
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 54, 366, 415
CseI GACGC 1 cut(s) 388
Csp6I GTAC 2 cut(s) 172, 678
CviAII CATG 2 cut(s) 58, 454
CviQI GTAC 2 cut(s) 172, 678
DdeI CTNAG 5 cut(s) 465, 522, 602, 720, 744
DpnI GATC 3 cut(s) 348, 861, 872
DpnII GATC 3 cut(s) 346, 859, 870
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 254, 788, 849
EarI CTCTTC 3 cut(s) 254, 788, 849
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 249, 581
Eco32I GATATC 1 cut(s) 900
Eco47I GGWCC 2 cut(s) 54, 415
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 305, 540
Eco88I CYCGRG 4 cut(s) 125, 237, 404, 726
Eco91I GGTNACC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 382
EcoRV GATATC 1 cut(s) 900
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 249, 581
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 2 cut(s) 61, 457
FaiI YATR 9 cut(s) 15, 59, 113, 134, 203, 455, 629, 662, 687
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FatI CATG 2 cut(s) 57, 453
Fnu4HI GCNGC 2 cut(s) 6, 49
FokI GGATG 1 cut(s) 70
FriOI GRGCYC 2 cut(s) 249, 581
Fsp4HI GCNGC 2 cut(s) 6, 49
GlaI GCGC 1 cut(s) 890
GluI GCNGC 2 cut(s) 6, 49
HaeIII GGCC 5 cut(s) 48, 131, 225, 367, 477
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 891
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 2 cut(s) 61, 457
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 6 cut(s) 35, 187, 358, 394, 495, 520
HphI GGTGA 2 cut(s) 275, 472
Hpy188I TCNGA 6 cut(s) 181, 324, 357, 468, 525, 605
Hpy188III TCNNGA 7 cut(s) 32, 125, 237, 239, 404, 692, 863
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 4 cut(s) 329, 406, 564, 652
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4V TGCA 4 cut(s) 615, 753, 788, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 785, 826
HpyF3I CTNAG 5 cut(s) 465, 522, 602, 720, 744
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 2 cut(s) 61, 457
HspAI GCGC 1 cut(s) 889
KpnI GGTACC 1 cut(s) 681
Kzo9I GATC 3 cut(s) 346, 859, 870
LguI GCTCTTC 2 cut(s) 254, 788
LmnI GCTCC 2 cut(s) 181, 776
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 92, 268
MaeIII GTNAC 3 cut(s) 143, 395, 634
MalI GATC 3 cut(s) 348, 861, 872
MboI GATC 3 cut(s) 346, 859, 870
MboII GAAGA 6 cut(s) 241, 290, 470, 805, 866, 880
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 2 cut(s) 249, 581
MluCI AATT 8 cut(s) 91, 332, 389, 502, 526, 608, 682, 911
MlyI GAGTC 3 cut(s) 196, 403, 514
MmeI TCCRAC 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 785, 826
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 346, 859, 870
NlaIII CATG 2 cut(s) 61, 457
NlaIV GGNNCC 3 cut(s) 658, 667, 679
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 395, 634
PaeR7I CTCGAG 3 cut(s) 125, 237, 404
PaqCI CACCTGC 1 cut(s) 820
PciSI GCTCTTC 2 cut(s) 254, 788
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 35, 358, 495
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 2 cut(s) 7, 50
PleI GAGTC 3 cut(s) 195, 402, 514
PpsI GAGTC 3 cut(s) 195, 402, 514
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 382
PspEI GGTNACC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 3 cut(s) 658, 667, 679
PspPI GGNCC 3 cut(s) 54, 366, 415
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 859
RsaI GTAC 2 cut(s) 173, 679
RsaNI GTAC 2 cut(s) 172, 678
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 2 cut(s) 254, 788
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 2 cut(s) 6, 49
Sau3AI GATC 3 cut(s) 346, 859, 870
Sau96I GGNCC 3 cut(s) 54, 366, 415
SchI GAGTC 3 cut(s) 196, 403, 514
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 2 cut(s) 249, 581
SfaNI GCATC 2 cut(s) 92, 268
Sfr274I CTCGAG 3 cut(s) 125, 237, 404
SinI GGWCC 2 cut(s) 54, 415
SlaI CTCGAG 3 cut(s) 125, 237, 404
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 4 cut(s) 125, 237, 404, 863
SmoI CTYRAG 4 cut(s) 125, 237, 404, 863
Sse9I AATT 8 cut(s) 91, 332, 389, 502, 526, 608, 682, 911
SsiI CCGC 2 cut(s) 49, 148
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 739
TaqI TCGA 6 cut(s) 126, 156, 238, 405, 693, 837
TasI AATT 8 cut(s) 91, 332, 389, 502, 526, 608, 682, 911
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 3 cut(s) 35, 358, 495
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 3 cut(s) 368, 379, 795
TseFI GTSAC 2 cut(s) 395, 634
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 395, 634
TspDTI ATGAA 3 cut(s) 100, 241, 726
TspRI CASTG 3 cut(s) 368, 379, 795
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 2 cut(s) 54, 415
XapI RAATTY 2 cut(s) 526, 608
XhoI CTCGAG 3 cut(s) 125, 237, 404
XmnI GAANNNNTTC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.