Rh4DG322100

Non-functional NADPH-dependent codeinone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000205
Physical Location & Seq
Forward (+)
54548325 .. 54549761
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG322100.1

Sequence Viewer

Length: 504 bp
ATGATGGCAGCAACCAAAATCCCAGAGGTGGTGTTAGGCTCCTCCAGTGGCCAAAGGACCATGCCTGTGCTCGGCTTTGGCACAGCAACTGACTACTTGGACCCGAATGCTTTGAAGACTGCGGTTCTTGAAGCCATAAAGCTGGGTTACAGACACTTCGACACGGCTTCCATATACACCTCAGAGCAGACTCTACGTGAAGCCGTGGCCGAAGCTATTAGCCTCGGTCTTATTGCCTCTAGAGACCAAGTGTTCATCACTTCAAAGATGTGGTGTAATGATGCTCATCCAGACTTGGTCATTCCTGCTCTAAAGAAATCACTTCAGAATCTTCGAATGGAGTACCTTGATCTGTATCTGATTCACTGGCGCATAAGTGGTAAGCCAGGGAAATTAGTGTACCCGCTAACCGAAGAGGACCTTGTGCCATTGGACTTGAAGGGCGTGTGGGCAGCCATGGAGGAGTCACAGAGACTTGGTCTCACACTCTCACCAAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

167

Amino Acids

18.46

Weight (kDa)

5.6

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 25 - 162 3.5e-22 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59950 AT1G59960
fragaria_vesca FvH4_2g01280 FvH4_3g20300 FvH4_3g20300 FvH4_4g25591 FvH4_4g25600 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25610 FvH4_4g25612 FvH4_4g25612 FvH4_4g25620 FvH4_4g35840
malus_domestica MD04G1063200.v1.1 MD10G1055100.v1.1 MD13G1014700.v1.1 MD13G1101200.v1.1 MD16G1101500.v1.1
prunus_persica Prupe.1G244700_v2.0.a1 Prupe.1G245100_v2.0.a1 Prupe.1G245400_v2.0.a1 Prupe.1G338900_v2.0.a1 Prupe.5G239400_v2.0.a1
pyrus_communis pycom13g08830 pycom16g01150 pycom16g08620
rosa_chinensis RchiOBHm_Chr1g0329311 RchiOBHm_Chr3g0483001 RchiOBHm_Chr4g0395321 RchiOBHm_Chr4g0433021 RchiOBHm_Chr4g0445371 RchiOBHm_Chr4g0445381 RchiOBHm_Chr4g0445421 RchiOBHm_Chr5g0023771 RchiOBHm_Chr5g0023781 RchiOBHm_Chr5g0068101 RchiOBHm_Chr5g0068111 RchiOBHm_Chr6g0281251 RchiOBHm_Chr7g0225361
rosa_laevigata RLG00000001827 RLG00000005742 RLG00000005743 RLG00000005744 RLG00000006791 RLG00000009629 RLG00000012981 RLG00000023308 RLG00000029902 RLG00000032738 RLG00000032739 RLG00000035956 RLG00000035957
rosa_multiflora Rmu_co7982306.1_g000001 Rmu_co8108716.1_g000001 Rmu_co8139310.1_g000001 Rmu_co8479199.1_g000001 Rmu_sc0000050.1_g000005 Rmu_sc0000050.1_g000006 Rmu_sc0000847.1_g000048 Rmu_sc0001372.1_g000008 Rmu_sc0001762.1_g000035 Rmu_sc0002791.1_g000001 Rmu_sc0002791.1_g000002 Rmu_sc0002791.1_g000005 Rmu_sc0003252.1_g000001 Rmu_sc0003351.1_g000013 Rmu_sc0006184.1_g000008 Rmu_sc0009518.1_g000006 Rmu_sc0010817.1_g000030 Rmu_sc0016867.1_g000002 Rmu_ssc0000027.1_g000010
rosa_roxburghii Rroxscaffold_1G00012480 Rroxscaffold_1G00012490 Rroxscaffold_1G00013240 Rroxscaffold_1G00013250 Rroxscaffold_1G00055410 Rroxscaffold_1G00055420 Rroxscaffold_3G00233890 Rroxscaffold_4G00321220 Rroxscaffold_5G00340230 Rroxscaffold_5G00340260 Rroxscaffold_6G00398750 Rroxscaffold_7G00187200
rosa_rugosa Rorug01G0078500 Rorug03G0202900 Rorug03G0358000 Rorug04G0361100 Rorug04G0361200 Rorug05G0079600 Rorug06G0140100 Rorug07G0228800
rosa_samantha Rh1AG096900 Rh1BG077500 Rh1CG093800 Rh1DG100300 Rh1DG100400 Rh4AG058500 Rh4AG318800 Rh4AG422300 Rh4AG422400 Rh4BG057300 Rh4BG326900 Rh4BG327000 Rh4BG434300 Rh4BG434600 Rh4CG063100 Rh4CG342300 Rh4CG342400 Rh4CG449100 Rh4CG449200 Rh4CG449300 Rh4CG449500 Rh4DG054000 Rh4DG054100 Rh4DG322100 Rh4DG430300 Rh4DG430400 Rh4DG430800 Rh5AG446700 Rh5AG446800 Rh5BG167000 Rh5BG465000 Rh5CG182600 Rh5DG168900 Rh5DG169000 Rh5DG478700 Rh6AG251900 Rh6BG255300 Rh6CG254700 Rh6DG246500 Rh7AG374400 Rh7BG363700 Rh7CG392700 Rh7CG392800 Rh7DG374500 Rh7DG374600
rosa_wichuraiana Rw0G001550 Rw0G005020 Rw1G007510 Rw1G007530 Rw1G007570 Rw3G022750 Rw4G004710 Rw4G027720 Rw4G036230 Rw4G036240 Rw5G015340 Rw6G021880 Rw7G031610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 122, 404
AcoI YGGCCR 2 cut(s) 49, 207
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 2 cut(s) 344, 401
AfiI CCNNNNNNNGG 2 cut(s) 28, 71
AgsI TTSAA 4 cut(s) 115, 131, 264, 439
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 2 cut(s) 142, 215
AluI AGCT 2 cut(s) 142, 215
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 3 cut(s) 237, 466, 485
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 2 cut(s) 49, 207
ApeKI GCWGC 2 cut(s) 8, 452
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 57, 100, 418
AsuHPI GGTGA 1 cut(s) 483
AsuII TTCGAA 1 cut(s) 334
AvaII GGWCC 3 cut(s) 57, 100, 418
BalI TGGCCA 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 20, 464
BceAI ACGGC 2 cut(s) 180, 188
BciT130I CCWGG 1 cut(s) 387
BcoDI GTCTC 3 cut(s) 237, 466, 485
BfaI CTAG 1 cut(s) 240
BisI GCNGC 2 cut(s) 9, 453
BlsI GCNGC 2 cut(s) 10, 454
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 3 cut(s) 57, 100, 418
BmgT120I GGNCC 3 cut(s) 57, 100, 418
BmiI GGNNCC 2 cut(s) 40, 102
BmrFI CCNGG 1 cut(s) 387
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 334
BsaAI YACGTR 1 cut(s) 197
BsaBI GATNNNNATC 2 cut(s) 285, 354
BsaI GGTCTC 2 cut(s) 237, 485
BsaJI CCNNGG 4 cut(s) 204, 223, 386, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 71
Bse1I ACTGG 2 cut(s) 45, 371
Bse8I GATNNNNATC 2 cut(s) 285, 354
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 204, 223, 386, 456
BseGI GGATG 1 cut(s) 286
BseJI GATNNNNATC 2 cut(s) 285, 354
BseLI CCNNNNNNNGG 2 cut(s) 28, 71
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 45, 371
BseRI GAGGAG 2 cut(s) 31, 476
BseXI GCAGC 2 cut(s) 20, 464
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 2 cut(s) 51, 209
BsiHKAI GWGCWC 1 cut(s) 72
BslI CCNNNNNNNGG 2 cut(s) 28, 71
BsmAI GTCTC 3 cut(s) 237, 466, 485
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 2 cut(s) 51, 209
Bso31I GGTCTC 2 cut(s) 237, 485
Bsp119I TTCGAA 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 1 cut(s) 349
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 2 cut(s) 122, 404
BspANI GGCC 2 cut(s) 51, 209
BspCNI CTCAG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 40, 102
BspT104I TTCGAA 1 cut(s) 334
BspTNI GGTCTC 2 cut(s) 237, 485
BsrI ACTGG 2 cut(s) 45, 371
BssECI CCNNGG 4 cut(s) 204, 223, 386, 456
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 408
BstBAI YACGTR 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 181
BstDSI CCRYGG 2 cut(s) 204, 456
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 3 cut(s) 237, 466, 485
BstMBI GATC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 20, 464
BstV2I GAAGAC 1 cut(s) 122
BstXI CCANNNNNNTGG 1 cut(s) 142
BsuRI GGCC 2 cut(s) 51, 209
BtgI CCRYGG 2 cut(s) 204, 456
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 2 cut(s) 52, 364
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 3 cut(s) 57, 100, 418
Csp6I GTAC 2 cut(s) 343, 400
CviAII CATG 2 cut(s) 61, 457
CviQI GTAC 2 cut(s) 343, 400
DdeI CTNAG 1 cut(s) 181
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 49, 207
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 2 cut(s) 237, 485
Eco47I GGWCC 3 cut(s) 57, 100, 418
Eco57I CTGAAG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 2 cut(s) 64, 460
FaiI YATR 7 cut(s) 62, 137, 173, 175, 374, 458, 502
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 2 cut(s) 60, 456
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 9, 453
FokI GGATG 1 cut(s) 273
Fsp4HI GCNGC 2 cut(s) 9, 453
FspBI CTAG 1 cut(s) 240
GlaI GCGC 1 cut(s) 371
GluI GCNGC 2 cut(s) 9, 453
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 28
HaeIII GGCC 2 cut(s) 51, 209
HhaI GCGC 1 cut(s) 372
Hin1II CATG 2 cut(s) 64, 460
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 4 cut(s) 190, 328, 361, 464
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 3 cut(s) 184, 327, 360
Hpy188III TCNNGA 3 cut(s) 128, 240, 290
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 196
HpyF3I CTNAG 1 cut(s) 181
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 2 cut(s) 64, 460
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 9 cut(s) 36, 58, 78, 128, 303, 318, 352, 372, 399
Lsp1109I GCAGC 2 cut(s) 20, 464
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 240
MaeII ACGT 1 cut(s) 196
MaeIII GTNAC 2 cut(s) 146, 465
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 3 cut(s) 127, 323, 425
MhlI GDGCHC 1 cut(s) 72
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 1 cut(s) 392
MluNI TGGCCA 1 cut(s) 51
MlyI GAGTC 2 cut(s) 184, 473
MnlI CCTC 7 cut(s) 19, 52, 190, 233, 247, 409, 454
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 1 cut(s) 387
NcoI CCATGG 1 cut(s) 456
NdeII GATC 1 cut(s) 349
NlaIII CATG 2 cut(s) 64, 460
NlaIV GGNNCC 2 cut(s) 40, 102
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 465
NspV TTCGAA 1 cut(s) 334
PctI GAATGC 1 cut(s) 112
PfeI GAWTC 2 cut(s) 328, 361
PflFI GACNNNGTC 2 cut(s) 296, 477
PkrI GCNGC 2 cut(s) 10, 454
PleI GAGTC 2 cut(s) 184, 472
PpsI GAGTC 2 cut(s) 184, 472
Ppu21I YACGTR 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 40, 102
PspPI GGNCC 3 cut(s) 57, 100, 418
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 89
PsyI GACNNNGTC 2 cut(s) 296, 477
RsaI GTAC 2 cut(s) 344, 401
RsaNI GTAC 2 cut(s) 343, 400
RseI CAYNNNNRTG 1 cut(s) 65
SatI GCNGC 2 cut(s) 9, 453
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 3 cut(s) 57, 100, 418
SchI GAGTC 2 cut(s) 184, 473
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 72
SetI ASST 7 cut(s) 30, 144, 182, 199, 217, 348, 423
SfaNI GCATC 1 cut(s) 271
SfuI TTCGAA 1 cut(s) 334
SinI GGWCC 3 cut(s) 57, 100, 418
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 1 cut(s) 392
SsiI CCGC 2 cut(s) 122, 404
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 1 cut(s) 456
TaiI ACGT 1 cut(s) 199
TaqI TCGA 2 cut(s) 159, 334
TaqII GACCGA 1 cut(s) 215
TasI AATT 1 cut(s) 392
TfiI GAWTC 2 cut(s) 328, 361
TscAI CASTG 2 cut(s) 52, 371
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 2 cut(s) 8, 452
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 1 cut(s) 244
TspRI CASTG 2 cut(s) 52, 371
Tth111I GACNNNGTC 2 cut(s) 296, 477
VpaK11BI GGWCC 3 cut(s) 57, 100, 418
XbaI TCTAGA 1 cut(s) 239
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.