RchiOBHm_Chr1g0328811

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000455
Physical Location & Seq
Forward (+)
18241752 .. 18243090
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55817

Sequence Viewer

Length: 912 bp
ATGTCAGATGTTGACTTGGGCGGTCTTGACCCCTTCACCCTATACTCCTGTATTCAGTCATTTCAGCCGAGACACTGTTCAAAACAGCAGGTCCCATCTGATCGAGCACTCACTGCTACAACAATGGGGGAAAAGAAGGTGACTATAATGATTCTGAAGGTAGACCTTCAGTGTGAAAAATGCTACTGGAAGGTCAAGAAAGTTCTCTGTAAATTCCCTCAAATACGAGACCAGACGTACGACGAGAAGAACAACCTGGTGATCATCAAAGTGGTCTGCTGTAGTCCTGAAAAGATCAGGGACAAGCTATGCTGCAAAGGAGGTGGCGCCATTAAGAGCATCGAGATCAAAGAGCCCGAGAAGCTCAAGCCTCCTTCTACGGGTGACAAATCCAAACAAACACAAGCTGCAGGTGAAACCAAAGGTGCCAAGGACGCGTCTAAAGAGAAACAAAAGGAAGATAAACCCGAAGACGGCGAGAAGCCGAAGGATGAGTCGAAGCAGAAGCGTGCTCCGGTTACTGCTTGTACTCCGCGGCCGGTGAACCCGTGCTGTATGGATTGTTATGGAGGGCGTCCTGGTGGTCCCTGCGAAACTCACCCTCCGCGGCGGCCGGTGAACCCGTGTTGTATGGATTGTTACGAAGGGCGTCCTAGTGGTCCCTGCGAAACTCGCCCTCCACGGCGGCCGGTGAACCCGTGTTGTATGGATTGTTACGAAGGGCGTCCTGGTGGTCCCTGCGAAACTGGGTATGGTTACGGTAGGCTTGCCCTTTACATGCAGTACGATGGCTACTATGGAAGGCCGGTCCATGATAGTTACGGCGGTGGGAGGAGCTATACTACTAGTTACTGCGTGACCCGCCCCGATTGTTTCAGTGAAGAAAATCCCCAAGCGTGCGCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

303

Amino Acids

33.66

Weight (kDa)

8.55

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51090 AT4G16380 AT4G16380 AT4G16380 AT4G16380
fragaria_vesca FvH4_3g00430 FvH4_3g00441 FvH4_3g00443 FvH4_3g00445
malus_domestica MD05G1360500.v1.1 MD08G1234400.v1.1 MD10G1337500.v1.1 MD10G1337700.v1.1 MD10G1337900.v1.1 MD10G1338100.v1.1
prunus_persica Prupe.4G004900_v2.0.a1 Prupe.4G004900_v2.0.a1 Prupe.4G005100_v2.0.a1
pyrus_communis pycom05g32730 pycom10g28690 pycom10g28710 pycom10g28730 pycom10g28760 pycom10g28770
rosa_chinensis RchiOBHm_Chr1g0328811 RchiOBHm_Chr1g0347291 RchiOBHm_Chr1g0347351 RchiOBHm_Chr2g0102091 RchiOBHm_Chr5g0000621
rosa_laevigata RLG00000005325 RLG00000017130 RLG00000028756 RLG00000028757 RLG00000028759 RLG00000028763 RLG00000030882
rosa_multiflora Rmu_co8114210.1_g000001 Rmu_co8238747.1_g000001 Rmu_co8428345.1_g000001 Rmu_sc0000157.1_g000022 Rmu_sc0000157.1_g000026 Rmu_sc0002705.1_g000026 Rmu_sc0002705.1_g000035 Rmu_sc0004156.1_g000004 Rmu_sc0008178.1_g000003 Rmu_sc0012465.1_g000001
rosa_roxburghii Rroxscaffold_1G00075580 Rroxscaffold_2G00140790 Rroxscaffold_3G00273490 Rroxscaffold_4G00307890 Rroxscaffold_4G00307920 Rroxscaffold_4G00307930
rosa_rugosa Rorug01G0186000 Rorug02G0104400.1 Rorug04G0385400 Rorug04G0385400 Rorug05G0290500 Rorug06G0427900
rosa_samantha Rh1AG094800 Rh1AG203100 Rh1AG203500 Rh1AG203900 Rh1BG169300 Rh1BG169500 Rh1CG188400 Rh1CG188800 Rh1CG189000 Rh1DG098100 Rh1DG199800 Rh1DG199900 Rh2AG152900 Rh2BG158600 Rh2BG158800 Rh2CG158000 Rh2DG158500 Rh5AG005200 Rh5BG006800 Rh5CG005600 Rh5DG005500 Rh7AG027500 Rh7BG026700 Rh7CG028600 Rh7DG028200
rosa_wichuraiana Rw1G007400 Rw1G017040 Rw1G017060 Rw1G017080 Rw1G017090 Rw2G011910 Rw2G011920 Rw5G000510 Rw7G002210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 401
Acc36I ACCTGC 2 cut(s) 79, 401
AccB1I GGYRCC 2 cut(s) 326, 425
AccI GTMKAC 1 cut(s) 162
AccII CGCG 3 cut(s) 437, 535, 607
AciI CCGC 9 cut(s) 21, 533, 535, 605, 607, 610, 685, 825, 862
AcoI YGGCCR 3 cut(s) 536, 611, 686
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 2 cut(s) 152, 176
AcyI GRCGYC 4 cut(s) 327, 574, 649, 724
AfaI GTAC 3 cut(s) 239, 529, 785
AfiI CCNNNNNNNGG 2 cut(s) 380, 473
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 81
AhlI ACTAGT 1 cut(s) 845
AjnI CCWGG 3 cut(s) 255, 577, 727
AluBI AGCT 4 cut(s) 307, 364, 407, 837
AluI AGCT 4 cut(s) 307, 364, 407, 837
Alw21I GWGCWC 2 cut(s) 109, 514
Alw26I GTCTC 2 cut(s) 64, 222
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 4 cut(s) 536, 611, 686, 803
ApeKI GCWGC 2 cut(s) 312, 407
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
AspLEI GCGC 2 cut(s) 329, 902
AspS9I GGNCC 5 cut(s) 91, 584, 659, 734, 808
AsuHPI GGTGA 9 cut(s) 28, 151, 271, 395, 425, 553, 590, 628, 703
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 5 cut(s) 91, 584, 659, 734, 808
BanI GGYRCC 2 cut(s) 326, 425
BanII GRGCYC 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 477
Bbv12I GWGCWC 2 cut(s) 109, 514
BbvI GCAGC 2 cut(s) 299, 394
BccI CCATC 3 cut(s) 103, 782, 911
BceAI ACGGC 3 cut(s) 490, 698, 838
BciT130I CCWGG 3 cut(s) 257, 579, 729
BclI TGATCA 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 64, 222
BcuI ACTAGT 1 cut(s) 845
BfaI CTAG 2 cut(s) 654, 846
BfmI CTRYAG 2 cut(s) 280, 408
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 2 cut(s) 79, 401
BisI GCNGC 6 cut(s) 313, 408, 536, 608, 611, 686
BlsI GCNGC 6 cut(s) 314, 409, 537, 609, 612, 687
Bme1390I CCNGG 3 cut(s) 257, 579, 729
Bme18I GGWCC 5 cut(s) 91, 584, 659, 734, 808
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 5 cut(s) 91, 584, 659, 734, 808
BmiI GGNNCC 6 cut(s) 93, 328, 427, 586, 661, 736
BmrFI CCNGG 3 cut(s) 257, 579, 729
BmrI ACTGGG 1 cut(s) 756
BmsI GCATC 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 756
BpiI GAAGAC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 350
BsaHI GRCGYC 4 cut(s) 327, 574, 649, 724
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 429, 533, 605, 680
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 661, 691
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 473
Bse118I RCCGGY 4 cut(s) 538, 613, 688, 805
Bse1I ACTGG 2 cut(s) 191, 751
BseBI CCWGG 3 cut(s) 257, 579, 729
BseDI CCNNGG 4 cut(s) 429, 533, 605, 680
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 2 cut(s) 380, 473
BseNI ACTGG 2 cut(s) 191, 751
BseRI GAGGAG 1 cut(s) 847
BseX3I CGGCCG 3 cut(s) 536, 611, 686
BseXI GCAGC 2 cut(s) 299, 394
Bsh1236I CGCG 3 cut(s) 437, 535, 607
Bsh1285I CGRYCG 3 cut(s) 539, 614, 689
BshFI GGCC 4 cut(s) 538, 613, 688, 805
BshNI GGYRCC 2 cut(s) 326, 425
BsiEI CGRYCG 3 cut(s) 539, 614, 689
BsiHKAI GWGCWC 2 cut(s) 109, 514
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 5 cut(s) 515, 539, 614, 689, 806
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 5 cut(s) 77, 314, 570, 645, 720
BslI CCNNNNNNNGG 2 cut(s) 380, 473
BsmAI GTCTC 2 cut(s) 64, 222
BsmFI GGGAC 5 cut(s) 77, 314, 570, 645, 720
BsnI GGCC 4 cut(s) 538, 613, 688, 805
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 3 cut(s) 109, 357, 514
Bsp143I GATC 4 cut(s) 100, 261, 294, 345
BspACI CCGC 9 cut(s) 21, 533, 535, 605, 607, 610, 685, 825, 862
BspANI GGCC 4 cut(s) 538, 613, 688, 805
BspFNI CGCG 3 cut(s) 437, 535, 607
BspLI GGNNCC 6 cut(s) 93, 328, 427, 586, 661, 736
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 2 cut(s) 79, 401
BspT107I GGYRCC 2 cut(s) 326, 425
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 4 cut(s) 538, 613, 688, 805
BsrI ACTGG 2 cut(s) 191, 751
BssAI RCCGGY 4 cut(s) 538, 613, 688, 805
BssECI CCNNGG 4 cut(s) 429, 533, 605, 680
BssMI GATC 4 cut(s) 100, 261, 294, 345
BssNI GRCGYC 4 cut(s) 327, 574, 649, 724
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 3 cut(s) 257, 579, 729
Bst4CI ACNGT 2 cut(s) 77, 761
BstACI GRCGYC 4 cut(s) 327, 574, 649, 724
BstAPI GCANNNNNTGC 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 510, 768, 898
BstDSI CCRYGG 3 cut(s) 533, 605, 680
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 3 cut(s) 437, 535, 607
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 2 cut(s) 329, 902
BstKTI GATC 4 cut(s) 103, 264, 297, 348
BstMAI GTCTC 2 cut(s) 64, 222
BstMBI GATC 4 cut(s) 100, 261, 294, 345
BstMCI CGRYCG 3 cut(s) 539, 614, 689
BstMWI GCNNNNNNNGC 5 cut(s) 113, 361, 434, 672, 861
BstNI CCWGG 3 cut(s) 257, 579, 729
BstNSI RCATGY 1 cut(s) 781
BstSCI CCNGG 3 cut(s) 255, 577, 727
BstSFI CTRYAG 2 cut(s) 280, 408
BstUI CGCG 3 cut(s) 437, 535, 607
BstV1I GCAGC 2 cut(s) 299, 394
BstV2I GAAGAC 1 cut(s) 477
BstZI CGGCCG 3 cut(s) 536, 611, 686
BsuRI GGCC 4 cut(s) 538, 613, 688, 805
BtgI CCRYGG 3 cut(s) 533, 605, 680
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 73, 111, 176, 883
BveI ACCTGC 2 cut(s) 79, 401
Cac8I GCNNGC 3 cut(s) 510, 768, 898
CfoI GCGC 2 cut(s) 329, 902
Cfr10I RCCGGY 4 cut(s) 538, 613, 688, 805
Cfr13I GGNCC 5 cut(s) 91, 584, 659, 734, 808
Cfr42I CCGCGG 2 cut(s) 536, 608
CseI GACGC 5 cut(s) 426, 443, 563, 638, 713
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 3 cut(s) 238, 528, 784
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 3 cut(s) 778, 812, 907
CviQI GTAC 3 cut(s) 238, 528, 784
DinI GGCGCC 1 cut(s) 328
DpnI GATC 4 cut(s) 102, 263, 296, 347
DpnII GATC 4 cut(s) 100, 261, 294, 345
EaeI YGGCCR 3 cut(s) 536, 611, 686
EagI CGGCCG 3 cut(s) 536, 611, 686
EclXI CGGCCG 3 cut(s) 536, 611, 686
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 5 cut(s) 91, 584, 659, 734, 808
Eco52I CGGCCG 3 cut(s) 536, 611, 686
Eco57I CTGAAG 2 cut(s) 152, 176
Eco88I CYCGRG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 3 cut(s) 255, 577, 727
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 357
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 3 cut(s) 781, 815, 910
FaqI GGGAC 5 cut(s) 77, 314, 570, 645, 720
FatI CATG 3 cut(s) 777, 811, 906
FauI CCCGC 1 cut(s) 869
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 6 cut(s) 313, 408, 536, 608, 611, 686
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 6 cut(s) 313, 408, 536, 608, 611, 686
FspBI CTAG 2 cut(s) 654, 846
GlaI GCGC 2 cut(s) 328, 901
GluI GCNGC 6 cut(s) 313, 408, 536, 608, 611, 686
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 4 cut(s) 538, 613, 688, 805
HapII CCGG 5 cut(s) 515, 539, 614, 689, 806
HgaI GACGC 5 cut(s) 426, 443, 563, 638, 713
HhaI GCGC 2 cut(s) 329, 902
Hin1I GRCGYC 4 cut(s) 327, 574, 649, 724
Hin1II CATG 3 cut(s) 781, 815, 910
Hin6I GCGC 2 cut(s) 327, 900
HinP1I GCGC 2 cut(s) 327, 900
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 151, 494
HpaII CCGG 5 cut(s) 515, 539, 614, 689, 806
HphI GGTGA 9 cut(s) 28, 151, 271, 395, 425, 553, 590, 628, 703
Hpy166II GTNNAC 5 cut(s) 13, 163, 544, 619, 694
Hpy188I TCNGA 3 cut(s) 7, 100, 156
Hpy188III TCNNGA 4 cut(s) 26, 196, 287, 343
Hpy8I GTNNAC 5 cut(s) 13, 163, 544, 619, 694
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 77, 761
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 3 cut(s) 315, 410, 781
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 361, 434, 672, 861
HpySE526I ACGT 1 cut(s) 236
Hsp92I GRCGYC 4 cut(s) 327, 574, 649, 724
Hsp92II CATG 3 cut(s) 781, 815, 910
HspAI GCGC 2 cut(s) 327, 900
KasI GGCGCC 1 cut(s) 326
Ksp22I TGATCA 1 cut(s) 261
KspI CCGCGG 2 cut(s) 536, 608
Kzo9I GATC 4 cut(s) 100, 261, 294, 345
LmnI GCTCC 2 cut(s) 517, 834
Lsp1109I GCAGC 2 cut(s) 299, 394
LweI GCATC 1 cut(s) 348
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 2 cut(s) 654, 846
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 9 cut(s) 139, 383, 517, 638, 713, 755, 818, 848, 856
MalI GATC 4 cut(s) 102, 263, 296, 347
MboI GATC 4 cut(s) 100, 261, 294, 345
MboII GAAGA 4 cut(s) 259, 470, 482, 893
MhlI GDGCHC 3 cut(s) 109, 357, 514
MluCI AATT 1 cut(s) 212
MluI ACGCGT 1 cut(s) 435
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 1 cut(s) 503
MnlI CCTC 7 cut(s) 228, 314, 381, 563, 612, 687, 825
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 535, 607
MspI CCGG 5 cut(s) 515, 539, 614, 689, 806
MspR9I CCNGG 3 cut(s) 257, 579, 729
MvaI CCWGG 3 cut(s) 257, 579, 729
MvnI CGCG 3 cut(s) 437, 535, 607
MwoI GCNNNNNNNGC 5 cut(s) 113, 361, 434, 672, 861
NarI GGCGCC 1 cut(s) 327
NdeII GATC 4 cut(s) 100, 261, 294, 345
NlaIII CATG 3 cut(s) 781, 815, 910
NlaIV GGNNCC 6 cut(s) 93, 328, 427, 586, 661, 736
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 3 cut(s) 139, 383, 856
NspI RCATGY 1 cut(s) 781
PaqCI CACCTGC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 151
Pfl23II CGTACG 1 cut(s) 237
PkrI GCNGC 6 cut(s) 314, 409, 537, 609, 612, 687
PleI GAGTC 1 cut(s) 502
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 502
PpuMI RGGWCCY 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 3 cut(s) 255, 577, 727
PspGI CCWGG 3 cut(s) 255, 577, 727
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 6 cut(s) 93, 328, 427, 586, 661, 736
PspPI GGNCC 5 cut(s) 91, 584, 659, 734, 808
PspPPI RGGWCCY 1 cut(s) 91
PstI CTGCAG 1 cut(s) 412
RsaI GTAC 3 cut(s) 239, 529, 785
RsaNI GTAC 3 cut(s) 238, 528, 784
RseI CAYNNNNRTG 1 cut(s) 269
SacII CCGCGG 2 cut(s) 536, 608
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 6 cut(s) 313, 408, 536, 608, 611, 686
Sau3AI GATC 4 cut(s) 100, 261, 294, 345
Sau96I GGNCC 5 cut(s) 91, 584, 659, 734, 808
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 257, 579, 729
SduI GDGCHC 3 cut(s) 109, 357, 514
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 1 cut(s) 348
SfcI CTRYAG 2 cut(s) 280, 408
SfoI GGCGCC 1 cut(s) 328
Sfr303I CCGCGG 2 cut(s) 536, 608
SgrBI CCGCGG 2 cut(s) 536, 608
SinI GGWCC 5 cut(s) 91, 584, 659, 734, 808
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SpeI ACTAGT 1 cut(s) 845
Sse9I AATT 1 cut(s) 212
SsiI CCGC 9 cut(s) 21, 533, 535, 605, 607, 610, 685, 825, 862
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 2 cut(s) 654, 846
StyD4I CCNGG 3 cut(s) 255, 577, 727
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 2 cut(s) 77, 761
TaiI ACGT 1 cut(s) 239
TaqI TCGA 3 cut(s) 103, 342, 497
TasI AATT 1 cut(s) 212
TatI WGTACW 1 cut(s) 527
TauI GCSGC 4 cut(s) 538, 610, 613, 688
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 4 cut(s) 80, 118, 176, 883
TseFI GTSAC 3 cut(s) 139, 383, 856
TseI GCWGC 2 cut(s) 312, 407
Tsp45I GTSAC 3 cut(s) 139, 383, 856
TspRI CASTG 4 cut(s) 80, 118, 176, 883
VpaK11BI GGWCC 5 cut(s) 91, 584, 659, 734, 808
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 781
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 2 cut(s) 654, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.