FvH4_1g23713

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000385
Physical Location & Seq
Reverse (-)
15571176 .. 15573754
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23713.t1

Sequence Viewer

Length: 1773 bp
ATGTCCGGAGAAGGAGTTTCGGTGGAGCAAGCTGTTGTTGGTGCCAAGAGGAGCCACAAGGAAGTACTTGCTGCCTTTGATGCACGGCTCGGAAAGGTGGAATCGGCCGTGGTCGAGTTTGACACTCGACTTGACCATGACGTCGTGACAAAGGGGGATCTCGCAGCCATGGTGGAAGAGTTGCGAGAGCAAATGCACGGCGCTCTCAACATTATGGCAGATAGGATCCAAGGGGAGGTCCGAGACAAGCTTGATCAAATATCCGCTGAGCTCACAGCCCTCCGAGAGGAAGTGAAGGAGGTCAAGGGAGATTGGGCGCTATGCAAAGAGGCTGCACTTAACAAGTCGCAAGGGCCAAAGGAGACGAAGGCATTGGACTCTTTCAAACCCAAATCCTTTAGTGGGAAGAGGGAAGCTAAAGAGCTTGACACCTTTCTATGGAACATGGAAAGGAGTATGGAAATCGAGCAAGGTACGTTCTCTCTCGATACTTGGGAAGAATTCAAGAACGACATCAAGTTGCATTTCTATCCCCAAAATGCTCAATATGAAGCCAAGGAGAAGCTTAGGTGGCTAAAGCAAACGGGTAGCGTCAAAGATTATGTCAACACCTTTGTTAACTTGCTATTCGAAGTGCCCAACATGACGGAGGAGGACAAGCTCATTTACTTCATGAGTGGATTACAAAGTTGGGCAAAGCTTGAGTTGCAAAGAAGACAAGTGCAAATCTTGTCACAAGCCATTGCCGTCGCCGAGTCCCTAATTGAGTTCAAAGCTAGCCACCAAGGCGACACTAAGTTCAAGGGGAAGAAGGTTAGCTATGAGAAGAGTGGGGGAGACGTGCCAAAGCCGAAGGAGAAGCCCGAGTCAAGCTACAAGCTGAAGGTGAAGAGGTTCGAGAAGCACGACAAGTCCGCAAACAAGACTTGGGAAATGAAGTGCTACTTATGCGACGTCCCTCATATGATGCGCGATTGCCCTCAAAAGAAGGCACTCAAAGCCATGACCTTGCAAGAGCCTAGGATCGAGGAAGAGGAAGATAATGAAGCAAGGATGGGATGCATCCGGTTGCTTAATGCCGTGAAGGCTAGCGTTCCTCAACCAAGGCGACAAGCGGAAGGTAGATCGCTTTACATCGATGTCAAGGCAAAAGACACGATGATTCGTGCCTTGGTGGATTGCGGAGCAACTCATAACTTCATGACGTTGGAAGAAGCAAGGAAGCTTGGCCTCCGAGTCACGAAGGAATCGGGCAGTGTGAAGACGGTGAATACACCTGCCACGCCCATTGTTGGAGTAGCACGCGATGTGGAGGTTCACATCGGCGCTTGGAAGGGGAAAGTCGATTTCACCGTGGTCAAGATGGATGATTATGGCGTGGTAATCGGCCTTGAATTCATGGACAAGGTACGAGCATTTCCCGTTCCATTCTATAACATCTTTTGCATTGTTGCGGATGGAGAATTGCCTTGCGTGGTGCCCGTGGCAAGGCAAGCAAGGGAGAAGACAAAGGCTCTATCCGTCATGCAACTCGCCAAAGCTTGGAAGAAGGGCGAGCCGACATTTCTAGCAACCCTCAAGATGGATGAGGAGGGAGAACGAACTCTATGTGAAGAAGGAGAAATGTTCCTTTGCCAAGGAGGAAGTGGATTTCCTAGGCCACAAAATCAAGGATGGAAAGCTCTTGATAGAGGAAAGCAAGGTGAAGGCCATTCAAGATTGGGAGCCGCCAACCAAGGTACCCGCACTTCGATCTTTCCTTGGCTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Chromo RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

591

Amino Acids

66.5

Weight (kDa)

8.14

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 162 - 229 3.6e-11 Retrotransposon gag protein
Ty3_capsid PF19259 164 - 258 1.4e-07 Ty3 transposon capsid-like protein
Asp_protease PF09668 375 - 471 8e-07 Aspartyl protease
gag-asp_proteas PF13975 379 - 468 6.1e-09 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 380 - 466 5.3e-08 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23713 FvH4_1g25744 FvH4_2g00261 FvH4_2g00262 FvH4_2g15681 FvH4_2g17851 FvH4_2g35550 FvH4_3g05280 FvH4_3g10531 FvH4_3g25910 FvH4_3g28953 FvH4_3g33371 FvH4_3g33536 FvH4_3g36090 FvH4_4g03471 FvH4_4g03480 FvH4_4g06701 FvH4_4g19351 FvH4_4g34281 FvH4_5g00040 FvH4_5g03811 FvH4_5g03812 FvH4_5g11822 FvH4_5g30921 FvH4_5g31701 FvH4_6g12161 FvH4_6g15222 FvH4_6g15223 FvH4_6g17701 FvH4_6g17710 FvH4_6g19301 FvH4_6g33011 FvH4_6g34501 FvH4_6g50161 FvH4_7g04111 FvH4_7g04120 FvH4_7g04121 FvH4_7g04122 FvH4_7g04123 FvH4_7g28231 FvH4_c7g00010
malus_domestica MD12G1084200.v1.1
prunus_persica Prupe.1G206200_v2.0.a1 Prupe.8G085600_v2.0.a1
pyrus_communis pycom01g11080 pycom02g20500 pycom09g18370 pycom12555g00210 pycom13g23120 pycom14g10590 pycom16g17620 pycom16g22750
rosa_chinensis RchiOBHm_Chr2g0137481 RchiOBHm_Chr6g0251961 RchiOBHm_Chr7g0185821
rosa_laevigata RLG00000005585 RLG00000010281 RLG00000010912 RLG00000030342 RLG00000034281
rosa_multiflora Rmu_sc0000096.1_g000010 Rmu_sc0002481.1_g000045 Rmu_sc0003598.1_g000009 Rmu_sc0003863.1_g000019 Rmu_sc0003973.1_g000002 Rmu_sc0004823.1_g000050 Rmu_sc0005621.1_g000007 Rmu_sc0006537.1_g000030 Rmu_sc0006608.1_g000014 Rmu_sc0008506.1_g000007 Rmu_sc0014428.1_g000001 Rmu_sc0035437.1_g000001 Rmu_ssc0000396.1_g000022
rosa_roxburghii Rroxscaffold_175G00432300 Rroxscaffold_17G00435390 Rroxscaffold_27G00446590 Rroxscaffold_29G00441820 Rroxscaffold_31G00438110 Rroxscaffold_32G00442750 Rroxscaffold_33G00439810 Rroxscaffold_37G00445160 Rroxscaffold_4G00306500 Rroxscaffold_4G00327700 Rroxscaffold_4G00332630 Rroxscaffold_52G00439360 Rroxscaffold_63G00444420 Rroxscaffold_76G00448580 Rroxscaffold_7G00156270
rosa_rugosa Rorug03G0295200
rosa_samantha Rh1BG433700 Rh1CG115400 Rh2AG676300 Rh2CG341800 Rh2DG381400 Rh3BG370100 Rh3BG370200 Rh4AG441600 Rh4BG076500 Rh4BG107400 Rh5BG005600 Rh6BG010100 Rh7BG000200
rosa_wichuraiana Rw0G003820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1285
AasI GACNNNNNNGTC 1 cut(s) 140
AatII GACGTC 2 cut(s) 144, 957
Acc36I ACCTGC 1 cut(s) 1285
Acc65I GGTACC 1 cut(s) 1739
AccB1I GGYRCC 3 cut(s) 41, 1477, 1739
AccB7I CCANNNNNTGG 1 cut(s) 1542
AccII CGCG 2 cut(s) 972, 1305
AccIII TCCGGA 1 cut(s) 5
AciI CCGC 7 cut(s) 264, 915, 1115, 1182, 1454, 1728, 1744
AclWI GGATC 4 cut(s) 165, 220, 233, 1031
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 2 cut(s) 500, 1394
AcuI CTGAAG 1 cut(s) 902
AcyI GRCGYC 2 cut(s) 141, 954
AfaI GTAC 4 cut(s) 66, 475, 1410, 1741
AfiI CCNNNNNNNGG 9 cut(s) 235, 286, 402, 438, 1292, 1488, 1542, 1582, 1766
AgsI TTSAA 6 cut(s) 385, 505, 772, 802, 1394, 1716
AjiI CACGTC 1 cut(s) 841
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 3 cut(s) 237, 356, 831
AlwI GGATC 4 cut(s) 165, 220, 233, 1031
Ama87I CYCGRG 1 cut(s) 863
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 6 cut(s) 105, 353, 1228, 1387, 1658, 1708
ApeKI GCWGC 3 cut(s) 71, 164, 332
ApoI RAATTY 2 cut(s) 500, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
Asp700I GAANNNNTTC 1 cut(s) 893
Asp718I GGTACC 1 cut(s) 1739
AspA2I CCTAGG 2 cut(s) 1019, 1655
AspLEI GCGC 4 cut(s) 203, 319, 972, 1328
AspS9I GGNCC 2 cut(s) 238, 353
AsuHPI GGTGA 4 cut(s) 898, 1279, 1342, 1715
AsuII TTCGAA 1 cut(s) 630
AsuNHI GCTAGC 2 cut(s) 776, 1088
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 1 cut(s) 238
AvrII CCTAGG 2 cut(s) 1019, 1655
BaeGI GKGCMC 2 cut(s) 639, 1482
BaeI ACNNNNGTAYC 2 cut(s) 1723, 1756
BamHI GGATCC 1 cut(s) 225
BanI GGYRCC 3 cut(s) 41, 1477, 1739
BanII GRGCYC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 1732, 1764
BbsI GAAGAC 3 cut(s) 721, 1268, 1511
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 58, 176, 319
BccI CCATC 5 cut(s) 1048, 1357, 1451, 1576, 1668
BceAI ACGGC 5 cut(s) 92, 101, 214, 731, 1064
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 237, 356, 831
BfaI CTAG 5 cut(s) 777, 1020, 1089, 1568, 1656
BfoI RGCGCY 3 cut(s) 204, 320, 1329
BfuAI ACCTGC 1 cut(s) 1285
BglI GCCNNNNNGGC 2 cut(s) 786, 1085
BisI GCNGC 4 cut(s) 72, 165, 333, 1728
BlnI CCTAGG 2 cut(s) 1019, 1655
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 4 cut(s) 73, 166, 334, 1729
BmcAI AGTACT 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 863
BmgBI CACGTC 1 cut(s) 841
BmgT120I GGNCC 2 cut(s) 238, 353
BmiI GGNNCC 6 cut(s) 43, 53, 227, 1479, 1726, 1741
BmsI GCATC 4 cut(s) 70, 957, 1049, 1071
BmtI GCTAGC 2 cut(s) 780, 1092
BpiI GAAGAC 3 cut(s) 721, 1268, 1511
Bpu10I CCTNAGC 1 cut(s) 566
Bpu1102I GCTNAGC 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 722, 1562
Bsa29I ATCGAT 1 cut(s) 1137
BsaHI GRCGYC 2 cut(s) 141, 954
BsaWI WCCGGW 2 cut(s) 5, 1065
BsaXI ACNNNNNCTCC 3 cut(s) 6, 30, 36
Bsc4I CCNNNNNNNGG 9 cut(s) 235, 286, 402, 438, 1292, 1488, 1542, 1582, 1766
Bse3DI GCAATG 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 5
BseCI ATCGAT 1 cut(s) 1137
BseGI GGATG 7 cut(s) 1059, 1062, 1064, 1372, 1462, 1591, 1679
BseLI CCNNNNNNNGG 9 cut(s) 235, 286, 402, 438, 1292, 1488, 1542, 1582, 1766
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 1 cut(s) 258
BseRI GAGGAG 3 cut(s) 64, 665, 1604
BseSI GKGCMC 2 cut(s) 639, 1482
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 58, 176, 319
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 2 cut(s) 972, 1305
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 6 cut(s) 107, 355, 1230, 1389, 1660, 1710
BshNI GGYRCC 3 cut(s) 41, 1477, 1739
BshVI ATCGAT 1 cut(s) 1137
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 2 cut(s) 6, 1066
BslFI GGGAC 2 cut(s) 742, 941
BslI CCNNNNNNNGG 9 cut(s) 235, 286, 402, 438, 1292, 1488, 1542, 1582, 1766
BsmAI GTCTC 3 cut(s) 237, 356, 831
BsmBI CGTCTC 2 cut(s) 356, 831
BsmFI GGGAC 2 cut(s) 742, 941
BsnI GGCC 6 cut(s) 107, 355, 1230, 1389, 1660, 1710
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 3 cut(s) 273, 639, 1482
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 7 cut(s) 264, 915, 1115, 1182, 1454, 1728, 1744
BspANI GGCC 6 cut(s) 107, 355, 1230, 1389, 1660, 1710
BspCNI CTCAG 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 1137
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 2 cut(s) 972, 1305
BspHI TCATGA 2 cut(s) 672, 1200
BspLI GGNNCC 6 cut(s) 43, 53, 227, 1479, 1726, 1741
BspMI ACCTGC 1 cut(s) 1285
BspOI GCTAGC 2 cut(s) 780, 1092
BspPI GGATC 4 cut(s) 165, 220, 233, 1031
BspT104I TTCGAA 1 cut(s) 630
BspT107I GGYRCC 3 cut(s) 41, 1477, 1739
BsrDI GCAATG 1 cut(s) 741
BssMI GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
BssNI GRCGYC 2 cut(s) 141, 954
Bst4CI ACNGT 2 cut(s) 1267, 1354
Bst6I CTCTTC 5 cut(s) 171, 401, 821, 884, 1026
BstACI GRCGYC 2 cut(s) 141, 954
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 6 cut(s) 30, 778, 1090, 1303, 1494, 1556
BstDEI CTNAG 3 cut(s) 267, 566, 795
BstDSI CCRYGG 4 cut(s) 108, 168, 1353, 1482
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 7 cut(s) 1059, 1062, 1064, 1372, 1462, 1591, 1679
BstFNI CGCG 2 cut(s) 972, 1305
BstH2I RGCGCY 3 cut(s) 204, 320, 1329
BstHHI GCGC 4 cut(s) 203, 319, 972, 1328
BstKTI GATC 6 cut(s) 160, 228, 256, 1026, 1127, 1755
BstMAI GTCTC 3 cut(s) 237, 356, 831
BstMBI GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 8 cut(s) 80, 571, 706, 786, 948, 998, 1085, 1493
BstSLI GKGCMC 2 cut(s) 639, 1482
BstUI CGCG 2 cut(s) 972, 1305
BstV1I GCAGC 3 cut(s) 58, 176, 319
BstV2I GAAGAC 3 cut(s) 721, 1268, 1511
BstX2I RGATCY 2 cut(s) 157, 225
BstYI RGATCY 2 cut(s) 157, 225
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 1137
BsuRI GGCC 6 cut(s) 107, 355, 1230, 1389, 1660, 1710
BsuTUI ATCGAT 1 cut(s) 1137
BtgI CCRYGG 4 cut(s) 108, 168, 1353, 1482
BtgZI GCGATG 1 cut(s) 1320
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 7 cut(s) 1059, 1062, 1064, 1372, 1462, 1591, 1679
BtsI GCAGTG 1 cut(s) 1261
BtsIMutI CAGTG 1 cut(s) 1261
BveI ACCTGC 1 cut(s) 1285
Cac8I GCNNGC 6 cut(s) 30, 778, 1090, 1303, 1494, 1556
CciI TCATGA 2 cut(s) 672, 1200
CfoI GCGC 4 cut(s) 203, 319, 972, 1328
Cfr13I GGNCC 2 cut(s) 238, 353
ClaI ATCGAT 1 cut(s) 1137
CseI GACGC 1 cut(s) 580
Csp6I GTAC 4 cut(s) 65, 474, 1409, 1740
CspCI CAANNNNNGTGG 2 cut(s) 1270, 1305
CviAII CATG 9 cut(s) 137, 169, 445, 643, 673, 1003, 1201, 1399, 1525
CviQI GTAC 4 cut(s) 65, 474, 1409, 1740
DdeI CTNAG 3 cut(s) 267, 566, 795
DpnI GATC 6 cut(s) 159, 227, 255, 1025, 1126, 1754
DpnII GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 5 cut(s) 171, 401, 821, 884, 1026
EarI CTCTTC 5 cut(s) 171, 401, 821, 884, 1026
Ecl136II GAGCTC 1 cut(s) 271
EclXI CGGCCG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 238
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 271
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoRI GAATTC 2 cut(s) 500, 1394
EcoT22I ATGCAT 1 cut(s) 1064
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 2 cut(s) 356, 831
FaeI CATG 9 cut(s) 140, 172, 448, 646, 676, 1006, 1204, 1402, 1528
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 2 cut(s) 742, 941
FatI CATG 9 cut(s) 136, 168, 444, 642, 672, 1002, 1200, 1398, 1524
FauI CCCGC 1 cut(s) 1751
FauNDI CATATG 1 cut(s) 963
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 4 cut(s) 72, 165, 333, 1728
FokI GGATG 7 cut(s) 1049, 1066, 1071, 1379, 1469, 1598, 1686
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 72, 165, 333, 1728
FspBI CTAG 5 cut(s) 777, 1020, 1089, 1568, 1656
GlaI GCGC 4 cut(s) 202, 318, 971, 1327
GluI GCNGC 4 cut(s) 72, 165, 333, 1728
HaeII RGCGCY 3 cut(s) 204, 320, 1329
HaeIII GGCC 6 cut(s) 107, 355, 1230, 1389, 1660, 1710
HapII CCGG 2 cut(s) 6, 1066
HgaI GACGC 1 cut(s) 580
HhaI GCGC 4 cut(s) 203, 319, 972, 1328
Hin1I GRCGYC 2 cut(s) 141, 954
Hin1II CATG 9 cut(s) 140, 172, 448, 646, 676, 1006, 1204, 1402, 1528
Hin6I GCGC 4 cut(s) 201, 317, 970, 1326
HinP1I GCGC 4 cut(s) 201, 317, 970, 1326
HincII GTYRAC 2 cut(s) 607, 619
HindII GTYRAC 2 cut(s) 607, 619
HindIII AAGCTT 5 cut(s) 248, 563, 698, 1223, 1539
HinfI GANTC 7 cut(s) 101, 377, 755, 866, 1162, 1236, 1247
HpaI GTTAAC 1 cut(s) 619
HpaII CCGG 2 cut(s) 6, 1066
HphI GGTGA 4 cut(s) 898, 1279, 1342, 1715
Hpy166II GTNNAC 3 cut(s) 607, 619, 1318
Hpy188I TCNGA 4 cut(s) 92, 242, 284, 1235
Hpy8I GTNNAC 3 cut(s) 607, 619, 1318
Hpy99I CGWCG 3 cut(s) 146, 752, 956
HpyCH4III ACNGT 2 cut(s) 1267, 1354
HpyCH4IV ACGT 5 cut(s) 141, 476, 840, 954, 1205
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 571, 706, 786, 948, 998, 1085, 1493
HpyF3I CTNAG 3 cut(s) 267, 566, 795
HpySE526I ACGT 5 cut(s) 141, 476, 840, 954, 1205
Hsp92I GRCGYC 2 cut(s) 141, 954
Hsp92II CATG 9 cut(s) 140, 172, 448, 646, 676, 1006, 1204, 1402, 1528
HspAI GCGC 4 cut(s) 201, 317, 970, 1326
Kpn2I TCCGGA 1 cut(s) 5
KpnI GGTACC 1 cut(s) 1743
Ksp22I TGATCA 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 619
Kzo9I GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
LmnI GCTCC 4 cut(s) 25, 51, 1184, 1724
LpnPI CCDG 3 cut(s) 19, 1079, 1290
Lsp1109I GCAGC 3 cut(s) 58, 176, 319
LweI GCATC 4 cut(s) 70, 957, 1049, 1071
MaeI CTAG 5 cut(s) 777, 1020, 1089, 1568, 1656
MaeII ACGT 5 cut(s) 141, 476, 840, 954, 1205
MaeIII GTNAC 3 cut(s) 145, 732, 1237
MalI GATC 6 cut(s) 159, 227, 255, 1025, 1126, 1754
MboI GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
MflI RGATCY 2 cut(s) 157, 225
MhlI GDGCHC 3 cut(s) 273, 639, 1482
MluCI AATT 4 cut(s) 500, 762, 1394, 1463
MlyI GAGTC 4 cut(s) 371, 764, 875, 1245
MmeI TCCRAC 2 cut(s) 1188, 1273
Mph1103I ATGCAT 1 cut(s) 1064
MroI TCCGGA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 4 cut(s) 339, 618, 1074, 1771
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 2 cut(s) 6, 1066
MvnI CGCG 2 cut(s) 972, 1305
MwoI GCNNNNNNNGC 8 cut(s) 80, 571, 706, 786, 948, 998, 1085, 1493
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 963
NdeII GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
NheI GCTAGC 2 cut(s) 776, 1088
NlaIII CATG 9 cut(s) 140, 172, 448, 646, 676, 1006, 1204, 1402, 1528
NlaIV GGNNCC 6 cut(s) 43, 53, 227, 1479, 1726, 1741
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 3 cut(s) 145, 732, 1237
NsiI ATGCAT 1 cut(s) 1064
NspV TTCGAA 1 cut(s) 630
PagI TCATGA 2 cut(s) 672, 1200
PaqCI CACCTGC 1 cut(s) 1285
PcsI WCGNNNNNNNCGW 2 cut(s) 903, 1350
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 3 cut(s) 101, 1162, 1247
PflMI CCANNNNNTGG 1 cut(s) 1542
PkrI GCNGC 4 cut(s) 73, 166, 334, 1729
PleI GAGTC 4 cut(s) 371, 763, 874, 1244
PpsI GAGTC 4 cut(s) 371, 763, 874, 1244
Psp124BI GAGCTC 1 cut(s) 273
PspN4I GGNNCC 6 cut(s) 43, 53, 227, 1479, 1726, 1741
PspPI GGNCC 2 cut(s) 238, 353
PsuI RGATCY 2 cut(s) 157, 225
RsaI GTAC 4 cut(s) 66, 475, 1410, 1741
RsaNI GTAC 4 cut(s) 65, 474, 1409, 1740
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 4 cut(s) 339, 618, 1074, 1771
SatI GCNGC 4 cut(s) 72, 165, 333, 1728
Sau3AI GATC 6 cut(s) 157, 225, 253, 1023, 1124, 1752
Sau96I GGNCC 2 cut(s) 238, 353
ScaI AGTACT 1 cut(s) 66
SchI GAGTC 4 cut(s) 371, 764, 875, 1245
SduI GDGCHC 3 cut(s) 273, 639, 1482
SfaNI GCATC 4 cut(s) 70, 957, 1049, 1071
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 701, 1577
SmoI CTYRAG 2 cut(s) 701, 1577
Sse9I AATT 4 cut(s) 500, 762, 1394, 1463
SsiI CCGC 7 cut(s) 264, 915, 1115, 1182, 1454, 1728, 1744
SspMI CTAG 5 cut(s) 777, 1020, 1089, 1568, 1656
SstI GAGCTC 1 cut(s) 273
TaaI ACNGT 2 cut(s) 1267, 1354
TaiI ACGT 5 cut(s) 144, 479, 843, 957, 1208
TasI AATT 4 cut(s) 500, 762, 1394, 1463
TatI WGTACW 1 cut(s) 64
TauI GCSGC 1 cut(s) 1730
TfiI GAWTC 3 cut(s) 101, 1162, 1247
Tru1I TTAA 4 cut(s) 339, 618, 1074, 1771
Tru9I TTAA 4 cut(s) 339, 618, 1074, 1771
TscAI CASTG 1 cut(s) 1261
TseFI GTSAC 3 cut(s) 145, 732, 1237
TseI GCWGC 3 cut(s) 71, 164, 332
Tsp45I GTSAC 3 cut(s) 145, 732, 1237
TspDTI ATGAA 6 cut(s) 564, 661, 950, 1059, 1189, 1387
TspGWI ACGGA 2 cut(s) 662, 1510
TspRI CASTG 1 cut(s) 1261
Van91I CCANNNNNTGG 1 cut(s) 1542
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 2 cut(s) 500, 1394
XcmI CCANNNNNNNNNTGG 1 cut(s) 1643
XmaJI CCTAGG 2 cut(s) 1019, 1655
XmnI GAANNNNTTC 1 cut(s) 893
XspI CTAG 5 cut(s) 777, 1020, 1089, 1568, 1656
ZraI GACGTC 2 cut(s) 142, 955
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.