FvH4_7g28231

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000385
Physical Location & Seq
Reverse (-)
20960030 .. 20964007
3978 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28231.t1

Sequence Viewer

Length: 1785 bp
ATGGCAAGAGGAGGATCCAAGGTGACGCTAGTTGAGGGAGAGCAACACGATGCTGGGGAATCAGCATTGGCTGCCGGCACTCCTCATGTGGTTGAGCAACTAGTCGCACGCGAAGAGGCAAGCGAGCCGGAGGTTCCGAAGAAGAAACACTTGAAGGATGTCATAGCCGATATCGACTCACGACTATCCGAGGTGGAGGGGTTCGTGAGTGGCATGGAGACCCGAGTGGAGGAACTAGACCAAAGAGCGGATGGTCTAGAGGAGGAGGACTCCGTCATCCACGTCGCGATGAAGAGCATGACCATCAAGTTCGAGGATGCCTTGCGGGAGGAGGTTGCCAAGATTCGCGAGGAGTTCATGGGGGAACTCGCGACTATGCGACAAGTCCACGAGAGGGAAATGCAAAGCGCCCTTGCACGCATGGAGGAGATGCGTGAGGACTTAAACCTTTGCAAGAGGGCAGTAGCGTCGGGTGGTCCAAGCACGAGTGGTGCTGCCATGGAGGTAAGGCGCGTGGAAGTGCCGAAACCTAAGTCCTTTGGAGGCGCACGCAACGCACGAGAAGTTGATAACTTCTTATGGGGGCTGGATCAATATTTCGGAGCCGTGGGCATCGCGGAGGAGGACGCCAAGATCAAGAGCGCAGCTCTATACCTCACCGACACCGCCATGCTATGGTGGCGAAGGAAGAAGGGAGATATTGAAAAAGGTACGTGCACCATCACAACCTTTGACGATTTCACTAAGGAGCTTAAGAAACAATTTTATCCCGAGAATGCCGAGGAAGAAGCAAGGGCTCGTCTTCGACGGCTCAAGCAAACGAGCACCATCCGCGACTACATAAAGGAGTTCACAACCTTGGTGCTCGAAATCCCCGATATGTCCGATAAAGACGCCCTTTTCTACTTCATGGATGGTCTCCAACCATGGGCTAAGACCGAGCTACGACGTCGTGGGGTGCAAGACCTTGCGAGCGCCATAGCCGCGGCCGACGCCCTAATCGACTACTCAAATCCGAGGGAGTCCGTGAGCAAACCAAAAGAAAAGAAGGGTGGCTATGTCAAAGGTGGGGGAGACAAAAGCCAACGTAAAGAGGAGCCGCGACGACTTAAGGCCTCGGGATGGAACAAAGACAAGGGCAAGGCCGAGGAAGCAAAGGGGGCGAAGCGACCGAACGACAACAAGTGCTTCCTATGTGATGGGCCGCATTGGGCGAGGGATTGCCCACAAAGAAAAGCTCTAAGTGCACTCTTGGGGAGTCGTCAAGGTATGCGCCGAACCCTTGCTCTTTTGAAGTCCACTTATTATTGGCCAAGGATGCGCGATAGCGTCGAGGAGTACGTGAGGACATGTCTTGTGTGCCAACAAGACAAGGTAGTCCAGCAACAGCCGGGGGGCTTACTCGATCCTTTGCCTATTCCTGAGAGACCGTGGGAGAGTGTCTCGATGGACTTCATCACTTGCCTGCCGAAATCTGAGGGGTTCGGAAGCATCATTGTGGTAGTAGATCGATTTAGCAAGTATGCAACCTTCATGGCTGCATCTGCTGAGTGTTCCGTGGAGGAAACTGCGAGGCTATTCCTCCACAACGTGGTCAAGCTATGGGGAGTTCCGAGCAACATCGTGAGCGATCGAGATCCGCGCTTCACGGGAAGGTTTTGGACGGAGCTGTTCAAGATGTTGGGGACAGACTTGAACTTTTCGACAAGTTTTCATCCTCAATCCGATGGACAAACGGAGCGCATCAACTCACTCTTGGAGCTCTACCTGCGCGGCACTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

595

Amino Acids

67.02

Weight (kDa)

6.09

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 175 - 342 1.4e-13 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 214 - 309 2.8e-18 Retrotransposon gag protein
Integrase_H2C2 PF17921 422 - 458 4.6e-13 Integrase zinc binding domain
rve PF00665 475 - 573 3.2e-08 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23713 FvH4_1g25744 FvH4_2g00261 FvH4_2g00262 FvH4_2g15681 FvH4_2g17851 FvH4_2g35550 FvH4_3g05280 FvH4_3g10531 FvH4_3g25910 FvH4_3g28953 FvH4_3g33371 FvH4_3g33536 FvH4_3g36090 FvH4_4g03471 FvH4_4g03480 FvH4_4g06701 FvH4_4g19351 FvH4_4g34281 FvH4_5g00040 FvH4_5g03811 FvH4_5g03812 FvH4_5g11822 FvH4_5g30921 FvH4_5g31701 FvH4_6g12161 FvH4_6g15222 FvH4_6g15223 FvH4_6g17701 FvH4_6g17710 FvH4_6g19301 FvH4_6g33011 FvH4_6g34501 FvH4_6g50161 FvH4_7g04111 FvH4_7g04120 FvH4_7g04121 FvH4_7g04122 FvH4_7g04123 FvH4_7g28231 FvH4_c7g00010
malus_domestica MD12G1084200.v1.1
prunus_persica Prupe.1G206200_v2.0.a1 Prupe.8G085600_v2.0.a1
pyrus_communis pycom01g11080 pycom02g20500 pycom09g18370 pycom12555g00210 pycom13g23120 pycom14g10590 pycom16g17620 pycom16g22750
rosa_chinensis RchiOBHm_Chr2g0137481 RchiOBHm_Chr6g0251961 RchiOBHm_Chr7g0185821
rosa_laevigata RLG00000005585 RLG00000010281 RLG00000010912 RLG00000030342 RLG00000034281
rosa_multiflora Rmu_sc0000096.1_g000010 Rmu_sc0002481.1_g000045 Rmu_sc0003598.1_g000009 Rmu_sc0003863.1_g000019 Rmu_sc0003973.1_g000002 Rmu_sc0004823.1_g000050 Rmu_sc0005621.1_g000007 Rmu_sc0006537.1_g000030 Rmu_sc0006608.1_g000014 Rmu_sc0008506.1_g000007 Rmu_sc0014428.1_g000001 Rmu_sc0035437.1_g000001 Rmu_ssc0000396.1_g000022
rosa_roxburghii Rroxscaffold_175G00432300 Rroxscaffold_17G00435390 Rroxscaffold_27G00446590 Rroxscaffold_29G00441820 Rroxscaffold_31G00438110 Rroxscaffold_32G00442750 Rroxscaffold_33G00439810 Rroxscaffold_37G00445160 Rroxscaffold_4G00306500 Rroxscaffold_4G00327700 Rroxscaffold_4G00332630 Rroxscaffold_52G00439360 Rroxscaffold_63G00444420 Rroxscaffold_76G00448580 Rroxscaffold_7G00156270
rosa_rugosa Rorug03G0295200
rosa_samantha Rh1BG433700 Rh1CG115400 Rh2AG676300 Rh2CG341800 Rh2DG381400 Rh3BG370100 Rh3BG370200 Rh4AG441600 Rh4BG076500 Rh4BG107400 Rh5BG005600 Rh6BG010100 Rh7BG000200
rosa_wichuraiana Rw0G003820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1376
AatII GACGTC 1 cut(s) 952
Acc36I ACCTGC 1 cut(s) 1776
AccB7I CCANNNNNTGG 2 cut(s) 675, 1591
AccBSI CCGCTC 1 cut(s) 248
AclWI GGATC 5 cut(s) 9, 22, 597, 1400, 1631
AcoI YGGCCR 2 cut(s) 987, 1310
AcyI GRCGYC 4 cut(s) 627, 894, 949, 993
AdeI CACNNNGTG 2 cut(s) 1591, 1782
AfaI GTAC 2 cut(s) 712, 1340
AfiI CCNNNNNNNGG 7 cut(s) 229, 247, 394, 675, 928, 1122, 1591
AflII CTTAAG 2 cut(s) 752, 1109
AflIII ACRYGT 1 cut(s) 1349
AgsI TTSAA 5 cut(s) 154, 704, 1294, 1675, 1696
AhlI ACTAGT 1 cut(s) 100
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 7 cut(s) 647, 751, 943, 1238, 1600, 1669, 1762
AluI AGCT 7 cut(s) 647, 751, 943, 1238, 1600, 1669, 1762
Alw21I GWGCWC 5 cut(s) 719, 827, 867, 1249, 1764
Alw26I GTCTC 5 cut(s) 212, 923, 1068, 1420, 1447
Alw44I GTGCAC 2 cut(s) 715, 1245
AlwI GGATC 5 cut(s) 9, 22, 597, 1400, 1631
Ama87I CYCGRG 3 cut(s) 222, 770, 1117
AoxI GGCC 5 cut(s) 987, 1113, 1143, 1202, 1310
ApaLI GTGCAC 2 cut(s) 715, 1245
ApeKI GCWGC 4 cut(s) 71, 494, 644, 1538
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AspS9I GGNCC 2 cut(s) 476, 1202
AsuC2I CCSGG 1 cut(s) 1392
AsuHPI GGTGA 2 cut(s) 34, 649
AvaI CYCGRG 3 cut(s) 222, 770, 1117
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 2 cut(s) 719, 1249
BalI TGGCCA 1 cut(s) 1312
BamHI GGATCC 1 cut(s) 14
BanII GRGCYC 2 cut(s) 799, 1764
BarI GAAGNNNNNNTAC 2 cut(s) 1514, 1546
BauI CACGAG 3 cut(s) 389, 484, 558
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 5 cut(s) 719, 827, 867, 1249, 1764
BbvI GCAGC 4 cut(s) 58, 481, 656, 1525
BccI CCATC 9 cut(s) 245, 311, 728, 836, 908, 1116, 1193, 1441, 1721
BceAI ACGGC 2 cut(s) 590, 824
BcgI CGANNNNNNTGC 2 cut(s) 450, 484
BcnI CCSGG 1 cut(s) 1392
BcoDI GTCTC 5 cut(s) 212, 923, 1068, 1420, 1447
BcuI ACTAGT 1 cut(s) 100
BfaI CTAG 4 cut(s) 29, 101, 236, 257
BfoI RGCGCY 2 cut(s) 411, 978
BfrI CTTAAG 2 cut(s) 752, 1109
BfuAI ACCTGC 1 cut(s) 1776
BisI GCNGC 9 cut(s) 72, 495, 645, 984, 987, 1100, 1205, 1539, 1774
BlsI GCNGC 9 cut(s) 73, 496, 646, 985, 988, 1101, 1206, 1540, 1775
Bme1390I CCNGG 1 cut(s) 1392
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 3 cut(s) 222, 770, 1117
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 476, 1202
BmiI GGNNCC 4 cut(s) 16, 135, 604, 1098
BmrFI CCNGG 1 cut(s) 1392
BmsI GCATC 8 cut(s) 40, 307, 420, 621, 1308, 1500, 1550, 1752
BpiI GAAGAC 1 cut(s) 794
BplI GAGNNNNNCTC 6 cut(s) 254, 286, 631, 663, 1427, 1459
BpuEI CTTGAG 1 cut(s) 797
BpuMI CCSGG 1 cut(s) 1392
Bsa29I ATCGAT 1 cut(s) 1510
BsaAI YACGTR 2 cut(s) 714, 1342
BsaBI GATNNNNATC 1 cut(s) 1635
BsaHI GRCGYC 4 cut(s) 627, 894, 949, 993
BsaI GGTCTC 3 cut(s) 212, 923, 1420
Bsc4I CCNNNNNNNGG 7 cut(s) 229, 247, 394, 675, 928, 1122, 1591
Bse118I RCCGGY 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 1635
BseCI ATCGAT 1 cut(s) 1510
BseGI GGATG 9 cut(s) 163, 256, 276, 322, 828, 919, 1127, 1323, 1714
BseJI GATNNNNATC 1 cut(s) 1635
BseLI CCNNNNNNNGG 7 cut(s) 229, 247, 394, 675, 928, 1122, 1591
BseMII CTCAG 3 cut(s) 1413, 1467, 1539
BseSI GKGCMC 2 cut(s) 719, 1249
BseX3I CGGCCG 1 cut(s) 987
BseXI GCAGC 4 cut(s) 58, 481, 656, 1525
BseYI CCCAGC 1 cut(s) 53
Bsh1285I CGRYCG 3 cut(s) 990, 1172, 1633
BshFI GGCC 5 cut(s) 989, 1115, 1145, 1204, 1312
BshVI ATCGAT 1 cut(s) 1510
BsiEI CGRYCG 3 cut(s) 990, 1172, 1633
BsiHKAI GWGCWC 5 cut(s) 719, 827, 867, 1249, 1764
BsiHKCI CYCGRG 3 cut(s) 222, 770, 1117
BsiSI CCGG 3 cut(s) 75, 128, 1391
BslFI GGGAC 1 cut(s) 1699
BslI CCNNNNNNNGG 7 cut(s) 229, 247, 394, 675, 928, 1122, 1591
BsmAI GTCTC 5 cut(s) 212, 923, 1068, 1420, 1447
BsmFI GGGAC 1 cut(s) 1699
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 5 cut(s) 989, 1115, 1145, 1204, 1312
Bso31I GGTCTC 3 cut(s) 212, 923, 1420
BsoBI CYCGRG 3 cut(s) 222, 770, 1117
Bsp1286I GDGCHC 6 cut(s) 719, 799, 827, 867, 1249, 1764
Bsp143I GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
Bsp19I CCATGG 2 cut(s) 498, 926
Bsp68I TCGCGA 3 cut(s) 287, 348, 371
BspANI GGCC 5 cut(s) 989, 1115, 1145, 1204, 1312
BspCNI CTCAG 3 cut(s) 1414, 1468, 1540
BspDI ATCGAT 1 cut(s) 1510
BspLI GGNNCC 4 cut(s) 16, 135, 604, 1098
BspMI ACCTGC 1 cut(s) 1776
BspPI GGATC 5 cut(s) 9, 22, 597, 1400, 1631
BspQI GCTCTTC 1 cut(s) 287
BspTI CTTAAG 2 cut(s) 752, 1109
BspTNI GGTCTC 3 cut(s) 212, 923, 1420
BsrBI CCGCTC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
BssNI GRCGYC 4 cut(s) 627, 894, 949, 993
BssSI CACGAG 3 cut(s) 389, 484, 558
BssT1I CCWWGG 5 cut(s) 18, 498, 858, 926, 1313
Bst2BI CACGAG 3 cut(s) 389, 484, 558
Bst4CI ACNGT 1 cut(s) 1431
Bst6I CTCTTC 2 cut(s) 108, 287
BstACI GRCGYC 4 cut(s) 627, 894, 949, 993
BstAFI CTTAAG 2 cut(s) 752, 1109
BstAPI GCANNNNNTGC 1 cut(s) 71
BstBAI YACGTR 2 cut(s) 714, 1342
BstC8I GCNNGC 8 cut(s) 76, 109, 121, 125, 418, 550, 973, 1466
BstDEI CTNAG 7 cut(s) 531, 744, 933, 1241, 1422, 1476, 1548
BstDSI CCRYGG 6 cut(s) 498, 606, 926, 984, 1430, 1557
BstF5I GGATG 9 cut(s) 163, 256, 276, 322, 828, 919, 1127, 1323, 1714
BstH2I RGCGCY 2 cut(s) 411, 978
BstKTI GATC 7 cut(s) 17, 592, 636, 1408, 1510, 1633, 1639
BstMAI GTCTC 5 cut(s) 212, 923, 1068, 1420, 1447
BstMBI GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
BstMCI CGRYCG 3 cut(s) 990, 1172, 1633
BstNSI RCATGY 1 cut(s) 1353
BstSCI CCNGG 1 cut(s) 1390
BstSLI GKGCMC 2 cut(s) 719, 1249
BstV1I GCAGC 4 cut(s) 58, 481, 656, 1525
BstV2I GAAGAC 1 cut(s) 794
BstX2I RGATCY 2 cut(s) 14, 1636
BstYI RGATCY 2 cut(s) 14, 1636
BstZI CGGCCG 1 cut(s) 987
Bsu15I ATCGAT 1 cut(s) 1510
BsuRI GGCC 5 cut(s) 989, 1115, 1145, 1204, 1312
BsuTUI ATCGAT 1 cut(s) 1510
BtgI CCRYGG 6 cut(s) 498, 606, 926, 984, 1430, 1557
BtgZI GCGATG 2 cut(s) 302, 598
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 9 cut(s) 163, 256, 276, 322, 828, 919, 1127, 1323, 1714
BtuMI TCGCGA 3 cut(s) 287, 348, 371
BveI ACCTGC 1 cut(s) 1776
Cac8I GCNNGC 8 cut(s) 76, 109, 121, 125, 418, 550, 973, 1466
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 476, 1202
Cfr42I CCGCGG 1 cut(s) 987
ClaI ATCGAT 1 cut(s) 1510
CseI GACGC 6 cut(s) 34, 456, 635, 902, 1001, 1318
Csp6I GTAC 2 cut(s) 711, 1339
CviQI GTAC 2 cut(s) 711, 1339
DdeI CTNAG 7 cut(s) 531, 744, 933, 1241, 1422, 1476, 1548
DpnI GATC 7 cut(s) 16, 591, 635, 1407, 1509, 1632, 1638
DpnII GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
DraIII CACNNNGTG 2 cut(s) 1591, 1782
DrdI GACNNNNNNGTC 1 cut(s) 1376
DseDI GACNNNNNNGTC 1 cut(s) 1376
EaeI YGGCCR 2 cut(s) 987, 1310
EagI CGGCCG 1 cut(s) 987
Eam1104I CTCTTC 2 cut(s) 108, 287
EarI CTCTTC 2 cut(s) 108, 287
Ecl136II GAGCTC 1 cut(s) 1762
EclXI CGGCCG 1 cut(s) 987
Eco130I CCWWGG 5 cut(s) 18, 498, 858, 926, 1313
Eco147I AGGCCT 1 cut(s) 1115
Eco24I GRGCYC 2 cut(s) 799, 1764
Eco31I GGTCTC 3 cut(s) 212, 923, 1420
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 476
Eco52I CGGCCG 1 cut(s) 987
Eco53kI GAGCTC 1 cut(s) 1762
Eco88I CYCGRG 3 cut(s) 222, 770, 1117
EcoICRI GAGCTC 1 cut(s) 1762
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 5 cut(s) 18, 498, 858, 926, 1313
EcoT38I GRGCYC 2 cut(s) 799, 1764
ErhI CCWWGG 5 cut(s) 18, 498, 858, 926, 1313
FalI AAGNNNNNCTT 4 cut(s) 134, 166, 882, 914
FaqI GGGAC 1 cut(s) 1699
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 9 cut(s) 72, 495, 645, 984, 987, 1100, 1205, 1539, 1774
FokI GGATG 9 cut(s) 170, 263, 263, 329, 815, 926, 1134, 1330, 1701
FriOI GRGCYC 2 cut(s) 799, 1764
Fsp4HI GCNGC 9 cut(s) 72, 495, 645, 984, 987, 1100, 1205, 1539, 1774
FspBI CTAG 4 cut(s) 29, 101, 236, 257
GluI GCNGC 9 cut(s) 72, 495, 645, 984, 987, 1100, 1205, 1539, 1774
GsaI CCCAGC 1 cut(s) 57
HaeII RGCGCY 2 cut(s) 411, 978
HaeIII GGCC 5 cut(s) 989, 1115, 1145, 1204, 1312
HapII CCGG 3 cut(s) 75, 128, 1391
HgaI GACGC 6 cut(s) 34, 456, 635, 902, 1001, 1318
Hin1I GRCGYC 4 cut(s) 627, 894, 949, 993
HinfI GANTC 6 cut(s) 59, 176, 269, 343, 1022, 1258
HpaII CCGG 3 cut(s) 75, 128, 1391
HphI GGTGA 2 cut(s) 34, 649
Hpy166II GTNNAC 5 cut(s) 388, 717, 852, 1247, 1299
Hpy188I TCNGA 9 cut(s) 138, 190, 602, 886, 1017, 1477, 1487, 1614, 1726
Hpy8I GTNNAC 5 cut(s) 388, 717, 852, 1247, 1299
Hpy99I CGWCG 8 cut(s) 287, 472, 810, 951, 954, 995, 1107, 1334
HpyAV CCTTC 6 cut(s) 148, 678, 685, 1042, 1540, 1647
HpyCH4III ACNGT 1 cut(s) 1431
HpyCH4IV ACGT 6 cut(s) 282, 713, 949, 1087, 1341, 1590
HpyCH4V TGCA 8 cut(s) 403, 416, 453, 717, 961, 1247, 1526, 1541
HpyF3I CTNAG 7 cut(s) 531, 744, 933, 1241, 1422, 1476, 1548
HpySE526I ACGT 6 cut(s) 282, 713, 949, 1087, 1341, 1590
Hsp92I GRCGYC 4 cut(s) 627, 894, 949, 993
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
KspI CCGCGG 1 cut(s) 987
Kzo9I GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 6 cut(s) 602, 748, 1096, 1666, 1738, 1759
LpnPI CCDG 9 cut(s) 39, 88, 141, 572, 1394, 1404, 1434, 1478, 1781
Lsp1109I GCAGC 4 cut(s) 58, 481, 656, 1525
LweI GCATC 8 cut(s) 40, 307, 420, 621, 1308, 1500, 1550, 1752
MaeI CTAG 4 cut(s) 29, 101, 236, 257
MaeII ACGT 6 cut(s) 282, 713, 949, 1087, 1341, 1590
MaeIII GTNAC 1 cut(s) 22
MalI GATC 7 cut(s) 16, 591, 635, 1407, 1509, 1632, 1638
MbiI CCGCTC 1 cut(s) 248
MboI GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
MboII GAAGA 7 cut(s) 125, 151, 154, 304, 700, 794, 797
MflI RGATCY 2 cut(s) 14, 1636
MhlI GDGCHC 6 cut(s) 719, 799, 827, 867, 1249, 1764
MlsI TGGCCA 1 cut(s) 1312
MluCI AATT 1 cut(s) 761
MluNI TGGCCA 1 cut(s) 1312
MlyI GAGTC 4 cut(s) 170, 263, 1031, 1267
MmeI TCCRAC 1 cut(s) 946
Mox20I TGGCCA 1 cut(s) 1312
MroNI GCCGGC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 1312
MseI TTAA 3 cut(s) 443, 753, 1110
MslI CAYNNNNRTG 2 cut(s) 668, 1496
Msp20I TGGCCA 1 cut(s) 1312
MspA1I CMGCKG 1 cut(s) 986
MspCI CTTAAG 2 cut(s) 752, 1109
MspI CCGG 3 cut(s) 75, 128, 1391
MspR9I CCNGG 1 cut(s) 1392
Mva1269I GAATGC 1 cut(s) 781
NaeI GCCGGC 1 cut(s) 76
NciI CCSGG 1 cut(s) 1392
NcoI CCATGG 2 cut(s) 498, 926
NdeII GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
NgoMIV GCCGGC 1 cut(s) 74
NlaIV GGNNCC 4 cut(s) 16, 135, 604, 1098
NmeAIII GCCGAG 2 cut(s) 805, 1171
NmuCI GTSAC 1 cut(s) 22
NruI TCGCGA 3 cut(s) 287, 348, 371
NspI RCATGY 1 cut(s) 1353
PceI AGGCCT 1 cut(s) 1115
PciI ACATGT 1 cut(s) 1349
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 4 cut(s) 556, 873, 999, 1338
PctI GAATGC 1 cut(s) 781
PdiI GCCGGC 1 cut(s) 76
PfeI GAWTC 2 cut(s) 59, 343
PflFI GACNNNGTC 1 cut(s) 272
PflMI CCANNNNNTGG 2 cut(s) 675, 1591
PkrI GCNGC 9 cut(s) 73, 496, 646, 985, 988, 1101, 1206, 1540, 1775
Ple19I CGATCG 1 cut(s) 1633
PleI GAGTC 4 cut(s) 170, 263, 1030, 1266
PpsI GAGTC 4 cut(s) 170, 263, 1030, 1266
Ppu21I YACGTR 2 cut(s) 714, 1342
PscI ACATGT 1 cut(s) 1349
Psp124BI GAGCTC 1 cut(s) 1764
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 4 cut(s) 16, 135, 604, 1098
PspPI GGNCC 2 cut(s) 476, 1202
PsuI RGATCY 2 cut(s) 14, 1636
PsyI GACNNNGTC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 1633
RruI TCGCGA 3 cut(s) 287, 348, 371
RsaI GTAC 2 cut(s) 712, 1340
RsaNI GTAC 2 cut(s) 711, 1339
RseI CAYNNNNRTG 2 cut(s) 668, 1496
SacI GAGCTC 1 cut(s) 1764
SacII CCGCGG 1 cut(s) 987
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 3 cut(s) 443, 753, 1110
SatI GCNGC 9 cut(s) 72, 495, 645, 984, 987, 1100, 1205, 1539, 1774
Sau3AI GATC 7 cut(s) 14, 589, 633, 1405, 1507, 1630, 1636
Sau96I GGNCC 2 cut(s) 476, 1202
SchI GAGTC 4 cut(s) 170, 263, 1031, 1267
ScrFI CCNGG 1 cut(s) 1392
SduI GDGCHC 6 cut(s) 719, 799, 827, 867, 1249, 1764
SfaNI GCATC 8 cut(s) 40, 307, 420, 621, 1308, 1500, 1550, 1752
Sfr303I CCGCGG 1 cut(s) 987
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 2 cut(s) 668, 1496
SmlI CTYRAG 3 cut(s) 752, 812, 1109
SmoI CTYRAG 3 cut(s) 752, 812, 1109
SpeI ACTAGT 1 cut(s) 100
Sse9I AATT 1 cut(s) 761
SseBI AGGCCT 1 cut(s) 1115
SspI AATATT 1 cut(s) 596
SspMI CTAG 4 cut(s) 29, 101, 236, 257
SstI GAGCTC 1 cut(s) 1764
StuI AGGCCT 1 cut(s) 1115
StyD4I CCNGG 1 cut(s) 1390
StyI CCWWGG 5 cut(s) 18, 498, 858, 926, 1313
TaaI ACNGT 1 cut(s) 1431
TaiI ACGT 6 cut(s) 285, 716, 952, 1090, 1344, 1593
TaqII GACCGA 2 cut(s) 953, 1186
TasI AATT 1 cut(s) 761
TauI GCSGC 5 cut(s) 986, 989, 1102, 1207, 1776
TfiI GAWTC 2 cut(s) 59, 343
Tru1I TTAA 3 cut(s) 443, 753, 1110
Tru9I TTAA 3 cut(s) 443, 753, 1110
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 4 cut(s) 71, 494, 644, 1538
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 6 cut(s) 305, 346, 898, 1444, 1522, 1703
TspGWI ACGGA 5 cut(s) 262, 1015, 1546, 1679, 1751
Tth111I GACNNNGTC 1 cut(s) 272
Van91I CCANNNNNTGG 2 cut(s) 675, 1591
Vha464I CTTAAG 2 cut(s) 752, 1109
VneI GTGCAC 2 cut(s) 715, 1245
VpaK11BI GGWCC 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 256
XceI RCATGY 1 cut(s) 1353
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XspI CTAG 4 cut(s) 29, 101, 236, 257
ZraI GACGTC 1 cut(s) 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.