Chloroplastic group IIB intron splicing facilitator
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 857, 1126 |
| AccB1I | GGYRCC | 3 cut(s) | 715, 857, 1126 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 810 |
| AciI | CCGC | 7 cut(s) | 107, 475, 740, 770, 827, 1178, 1201 |
| AclWI | GGATC | 3 cut(s) | 581, 594, 1218 |
| AcsI | RAATTY | 3 cut(s) | 388, 419, 721 |
| AcuI | CTGAAG | 3 cut(s) | 728, 755, 1169 |
| AdeI | CACNNNGTG | 1 cut(s) | 455 |
| AfaI | GTAC | 7 cut(s) | 370, 639, 859, 918, 1007, 1022, 1128 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 335, 336, 409, 475, 596, 597, 810, 1207 |
| AflIII | ACRYGT | 1 cut(s) | 1261 |
| AgsI | TTSAA | 5 cut(s) | 37, 46, 210, 903, 1037 |
| AhlI | ACTAGT | 2 cut(s) | 371, 794 |
| AjnI | CCWGG | 2 cut(s) | 408, 994 |
| AluBI | AGCT | 7 cut(s) | 88, 236, 733, 816, 851, 923, 1154 |
| AluI | AGCT | 7 cut(s) | 88, 236, 733, 816, 851, 923, 1154 |
| Alw26I | GTCTC | 3 cut(s) | 141, 778, 1221 |
| AlwI | GGATC | 3 cut(s) | 581, 594, 1218 |
| AoxI | GGCC | 2 cut(s) | 225, 753 |
| ApeKI | GCWGC | 4 cut(s) | 233, 236, 378, 519 |
| ApoI | RAATTY | 3 cut(s) | 388, 419, 721 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 344, 1111 |
| Asp718I | GGTACC | 2 cut(s) | 857, 1126 |
| AspLEI | GCGC | 2 cut(s) | 328, 662 |
| AspS9I | GGNCC | 4 cut(s) | 225, 353, 446, 1180 |
| AsuC2I | CCSGG | 2 cut(s) | 200, 867 |
| AsuHPI | GGTGA | 5 cut(s) | 537, 628, 634, 816, 1153 |
| AsuNHI | GCTAGC | 1 cut(s) | 88 |
| AvaII | GGWCC | 3 cut(s) | 353, 446, 1180 |
| BaeGI | GKGCMC | 1 cut(s) | 470 |
| BamHI | GGATCC | 1 cut(s) | 586 |
| BanI | GGYRCC | 3 cut(s) | 715, 857, 1126 |
| BanII | GRGCYC | 3 cut(s) | 200, 531, 603 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 256, 288 |
| BbsI | GAAGAC | 2 cut(s) | 161, 791 |
| BbvI | GCAGC | 4 cut(s) | 223, 245, 390, 531 |
| BccI | CCATC | 5 cut(s) | 16, 20, 236, 590, 613 |
| BceAI | ACGGC | 1 cut(s) | 76 |
| BciT130I | CCWGG | 2 cut(s) | 410, 996 |
| BciVI | GTATCC | 1 cut(s) | 307 |
| BcnI | CCSGG | 2 cut(s) | 200, 867 |
| BcoDI | GTCTC | 3 cut(s) | 141, 778, 1221 |
| BcuI | ACTAGT | 2 cut(s) | 371, 794 |
| BfaI | CTAG | 7 cut(s) | 89, 372, 494, 726, 795, 1017, 1224 |
| BfmI | CTRYAG | 1 cut(s) | 237 |
| BfoI | RGCGCY | 1 cut(s) | 663 |
| BfuI | GTATCC | 1 cut(s) | 307 |
| BisI | GCNGC | 4 cut(s) | 234, 237, 379, 520 |
| BlsI | GCNGC | 4 cut(s) | 235, 238, 380, 521 |
| Bme1390I | CCNGG | 4 cut(s) | 200, 410, 867, 996 |
| Bme18I | GGWCC | 3 cut(s) | 353, 446, 1180 |
| BmgT120I | GGNCC | 4 cut(s) | 225, 353, 446, 1180 |
| BmiI | GGNNCC | 7 cut(s) | 226, 354, 588, 717, 859, 1114, 1128 |
| BmrFI | CCNGG | 4 cut(s) | 200, 410, 867, 996 |
| BmsI | GCATC | 2 cut(s) | 1206, 1229 |
| BmtI | GCTAGC | 1 cut(s) | 92 |
| BpiI | GAAGAC | 2 cut(s) | 161, 791 |
| BpmI | CTGGAG | 1 cut(s) | 953 |
| BpuEI | CTTGAG | 2 cut(s) | 158, 1134 |
| BpuMI | CCSGG | 2 cut(s) | 200, 867 |
| BsaI | GGTCTC | 1 cut(s) | 778 |
| BsaJI | CCNNGG | 4 cut(s) | 228, 968, 994, 1009 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 335, 336, 409, 475, 596, 597, 810, 1207 |
| Bse118I | RCCGGY | 1 cut(s) | 92 |
| Bse1I | ACTGG | 2 cut(s) | 936, 1104 |
| Bse3DI | GCAATG | 2 cut(s) | 573, 830 |
| BseBI | CCWGG | 2 cut(s) | 410, 996 |
| BseDI | CCNNGG | 4 cut(s) | 228, 968, 994, 1009 |
| BseGI | GGATG | 4 cut(s) | 55, 275, 978, 1197 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 335, 336, 409, 475, 596, 597, 810, 1207 |
| BseMI | GCAATG | 2 cut(s) | 573, 830 |
| BseMII | CTCAG | 2 cut(s) | 492, 672 |
| BseNI | ACTGG | 2 cut(s) | 936, 1104 |
| BseRI | GAGGAG | 2 cut(s) | 140, 527 |
| BseSI | GKGCMC | 1 cut(s) | 470 |
| BseXI | GCAGC | 4 cut(s) | 223, 245, 390, 531 |
| BseYI | CCCAGC | 1 cut(s) | 700 |
| BshFI | GGCC | 2 cut(s) | 227, 755 |
| BshNI | GGYRCC | 3 cut(s) | 715, 857, 1126 |
| BsiSI | CCGG | 3 cut(s) | 93, 200, 867 |
| BslFI | GGGAC | 3 cut(s) | 366, 385, 717 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 335, 336, 409, 475, 596, 597, 810, 1207 |
| BsmAI | GTCTC | 3 cut(s) | 141, 778, 1221 |
| BsmFI | GGGAC | 3 cut(s) | 366, 385, 717 |
| BsnI | GGCC | 2 cut(s) | 227, 755 |
| Bso31I | GGTCTC | 1 cut(s) | 778 |
| Bsp1286I | GDGCHC | 4 cut(s) | 200, 470, 531, 603 |
| Bsp1407I | TGTACA | 1 cut(s) | 916 |
| Bsp143I | GATC | 2 cut(s) | 586, 1210 |
| Bsp19I | CCATGG | 2 cut(s) | 228, 1009 |
| BspACI | CCGC | 7 cut(s) | 107, 475, 740, 770, 827, 1178, 1201 |
| BspANI | GGCC | 2 cut(s) | 227, 755 |
| BspCNI | CTCAG | 2 cut(s) | 493, 673 |
| BspLI | GGNNCC | 7 cut(s) | 226, 354, 588, 717, 859, 1114, 1128 |
| BspMAI | CTGCAG | 1 cut(s) | 241 |
| BspOI | GCTAGC | 1 cut(s) | 92 |
| BspPI | GGATC | 3 cut(s) | 581, 594, 1218 |
| BspQI | GCTCTTC | 1 cut(s) | 78 |
| BspT107I | GGYRCC | 3 cut(s) | 715, 857, 1126 |
| BspTNI | GGTCTC | 1 cut(s) | 778 |
| BsrDI | GCAATG | 2 cut(s) | 573, 830 |
| BsrFI | RCCGGY | 1 cut(s) | 92 |
| BsrGI | TGTACA | 1 cut(s) | 916 |
| BsrI | ACTGG | 2 cut(s) | 936, 1104 |
| BssAI | RCCGGY | 1 cut(s) | 92 |
| BssECI | CCNNGG | 4 cut(s) | 228, 968, 994, 1009 |
| BssMI | GATC | 2 cut(s) | 586, 1210 |
| BssT1I | CCWWGG | 3 cut(s) | 228, 968, 1009 |
| Bst2UI | CCWGG | 2 cut(s) | 410, 996 |
| Bst4CI | ACNGT | 1 cut(s) | 616 |
| Bst6I | CTCTTC | 1 cut(s) | 78 |
| BstAUI | TGTACA | 1 cut(s) | 916 |
| BstC8I | GCNNGC | 3 cut(s) | 90, 94, 731 |
| BstDEI | CTNAG | 2 cut(s) | 501, 681 |
| BstDSI | CCRYGG | 2 cut(s) | 228, 1009 |
| BstEII | GGTNACC | 1 cut(s) | 989 |
| BstF5I | GGATG | 4 cut(s) | 55, 275, 978, 1197 |
| BstH2I | RGCGCY | 1 cut(s) | 663 |
| BstHHI | GCGC | 2 cut(s) | 328, 662 |
| BstKTI | GATC | 2 cut(s) | 589, 1213 |
| BstMAI | GTCTC | 3 cut(s) | 141, 778, 1221 |
| BstMBI | GATC | 2 cut(s) | 586, 1210 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 233, 497 |
| BstNI | CCWGG | 2 cut(s) | 410, 996 |
| BstNSI | RCATGY | 1 cut(s) | 1265 |
| BstPI | GGTNACC | 1 cut(s) | 989 |
| BstSCI | CCNGG | 4 cut(s) | 198, 408, 865, 994 |
| BstSFI | CTRYAG | 1 cut(s) | 237 |
| BstSLI | GKGCMC | 1 cut(s) | 470 |
| BstV1I | GCAGC | 4 cut(s) | 223, 245, 390, 531 |
| BstV2I | GAAGAC | 2 cut(s) | 161, 791 |
| BstX2I | RGATCY | 1 cut(s) | 586 |
| BstYI | RGATCY | 1 cut(s) | 586 |
| BsuI | GTATCC | 1 cut(s) | 307 |
| BsuRI | GGCC | 2 cut(s) | 227, 755 |
| BtgI | CCRYGG | 2 cut(s) | 228, 1009 |
| BtgZI | GCGATG | 1 cut(s) | 659 |
| BtsCI | GGATG | 4 cut(s) | 55, 275, 978, 1197 |
| BtsI | GCAGTG | 3 cut(s) | 27, 486, 529 |
| BtsIMutI | CAGTG | 4 cut(s) | 27, 486, 529, 1097 |
| Cac8I | GCNNGC | 3 cut(s) | 90, 94, 731 |
| CfoI | GCGC | 2 cut(s) | 328, 662 |
| Cfr10I | RCCGGY | 1 cut(s) | 92 |
| Cfr13I | GGNCC | 4 cut(s) | 225, 353, 446, 1180 |
| Csp6I | GTAC | 7 cut(s) | 369, 638, 858, 917, 1006, 1021, 1127 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1119, 1154 |
| CviAII | CATG | 6 cut(s) | 229, 404, 604, 1010, 1159, 1262 |
| CviQI | GTAC | 7 cut(s) | 369, 638, 858, 917, 1006, 1021, 1127 |
| DdeI | CTNAG | 2 cut(s) | 501, 681 |
| DpnI | GATC | 2 cut(s) | 588, 1212 |
| DpnII | GATC | 2 cut(s) | 586, 1210 |
| DraI | TTTAAA | 1 cut(s) | 952 |
| DraIII | CACNNNGTG | 1 cut(s) | 455 |
| Eam1104I | CTCTTC | 1 cut(s) | 78 |
| EarI | CTCTTC | 1 cut(s) | 78 |
| Eco130I | CCWWGG | 3 cut(s) | 228, 968, 1009 |
| Eco24I | GRGCYC | 3 cut(s) | 200, 531, 603 |
| Eco31I | GGTCTC | 1 cut(s) | 778 |
| Eco47I | GGWCC | 3 cut(s) | 353, 446, 1180 |
| Eco57I | CTGAAG | 3 cut(s) | 728, 755, 1169 |
| Eco91I | GGTNACC | 1 cut(s) | 989 |
| EcoO65I | GGTNACC | 1 cut(s) | 989 |
| EcoRI | GAATTC | 1 cut(s) | 721 |
| EcoRII | CCWGG | 2 cut(s) | 408, 994 |
| EcoT14I | CCWWGG | 3 cut(s) | 228, 968, 1009 |
| EcoT22I | ATGCAT | 1 cut(s) | 1222 |
| EcoT38I | GRGCYC | 3 cut(s) | 200, 531, 603 |
| ErhI | CCWWGG | 3 cut(s) | 228, 968, 1009 |
| FaeI | CATG | 6 cut(s) | 232, 407, 607, 1013, 1162, 1265 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 253, 285, 1167, 1199 |
| FaqI | GGGAC | 3 cut(s) | 366, 385, 717 |
| FatI | CATG | 6 cut(s) | 228, 403, 603, 1009, 1158, 1261 |
| FauI | CCCGC | 2 cut(s) | 468, 733 |
| FauNDI | CATATG | 1 cut(s) | 1205 |
| Fnu4HI | GCNGC | 4 cut(s) | 234, 237, 379, 520 |
| FokI | GGATG | 4 cut(s) | 62, 262, 985, 1184 |
| FriOI | GRGCYC | 3 cut(s) | 200, 531, 603 |
| Fsp4HI | GCNGC | 4 cut(s) | 234, 237, 379, 520 |
| FspBI | CTAG | 7 cut(s) | 89, 372, 494, 726, 795, 1017, 1224 |
| GlaI | GCGC | 2 cut(s) | 327, 661 |
| GluI | GCNGC | 4 cut(s) | 234, 237, 379, 520 |
| GsaI | CCCAGC | 1 cut(s) | 704 |
| GsuI | CTGGAG | 1 cut(s) | 953 |
| HaeII | RGCGCY | 1 cut(s) | 663 |
| HaeIII | GGCC | 2 cut(s) | 227, 755 |
| HapII | CCGG | 3 cut(s) | 93, 200, 867 |
| HhaI | GCGC | 2 cut(s) | 328, 662 |
| Hin1II | CATG | 6 cut(s) | 232, 407, 607, 1013, 1162, 1265 |
| Hin6I | GCGC | 2 cut(s) | 326, 660 |
| HinP1I | GCGC | 2 cut(s) | 326, 660 |
| HincII | GTYRAC | 2 cut(s) | 58, 69 |
| HindII | GTYRAC | 2 cut(s) | 58, 69 |
| HindIII | AAGCTT | 2 cut(s) | 921, 1152 |
| HinfI | GANTC | 2 cut(s) | 42, 187 |
| HpaI | GTTAAC | 1 cut(s) | 69 |
| HpaII | CCGG | 3 cut(s) | 93, 200, 867 |
| HphI | GGTGA | 5 cut(s) | 537, 628, 634, 816, 1153 |
| Hpy166II | GTNNAC | 2 cut(s) | 58, 69 |
| Hpy188I | TCNGA | 6 cut(s) | 139, 146, 260, 301, 751, 885 |
| Hpy188III | TCNNGA | 4 cut(s) | 175, 191, 1187, 1224 |
| Hpy8I | GTNNAC | 2 cut(s) | 58, 69 |
| HpyAV | CCTTC | 9 cut(s) | 43, 279, 286, 454, 574, 690, 703, 730, 1193 |
| HpyCH4III | ACNGT | 1 cut(s) | 616 |
| HpyCH4V | TGCA | 4 cut(s) | 239, 566, 611, 1220 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 233, 497 |
| HpyF3I | CTNAG | 2 cut(s) | 501, 681 |
| Hsp92II | CATG | 6 cut(s) | 232, 407, 607, 1013, 1162, 1265 |
| HspAI | GCGC | 2 cut(s) | 326, 660 |
| KpnI | GGTACC | 2 cut(s) | 861, 1130 |
| KroI | GCCGGC | 1 cut(s) | 92 |
| KroNI | GCCGGC | 1 cut(s) | 94 |
| KspAI | GTTAAC | 1 cut(s) | 69 |
| Kzo9I | GATC | 2 cut(s) | 586, 1210 |
| LguI | GCTCTTC | 1 cut(s) | 78 |
| LmnI | GCTCC | 2 cut(s) | 5, 813 |
| Lsp1109I | GCAGC | 4 cut(s) | 223, 245, 390, 531 |
| LweI | GCATC | 2 cut(s) | 1206, 1229 |
| MaeI | CTAG | 7 cut(s) | 89, 372, 494, 726, 795, 1017, 1224 |
| MaeIII | GTNAC | 2 cut(s) | 822, 989 |
| MalI | GATC | 2 cut(s) | 588, 1212 |
| MboI | GATC | 2 cut(s) | 586, 1210 |
| MboII | GAAGA | 6 cut(s) | 95, 161, 264, 352, 791, 857 |
| MflI | RGATCY | 1 cut(s) | 586 |
| MhlI | GDGCHC | 4 cut(s) | 200, 470, 531, 603 |
| MluCI | AATT | 7 cut(s) | 388, 419, 721, 762, 1083, 1131, 1162 |
| MmeI | TCCRAC | 3 cut(s) | 630, 961, 1084 |
| Mph1103I | ATGCAT | 1 cut(s) | 1222 |
| MroNI | GCCGGC | 1 cut(s) | 92 |
| MroXI | GAANNNNTTC | 2 cut(s) | 344, 1111 |
| MseI | TTAA | 3 cut(s) | 68, 315, 951 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1073, 1266 |
| MspA1I | CMGCKG | 1 cut(s) | 236 |
| MspI | CCGG | 3 cut(s) | 93, 200, 867 |
| MspR9I | CCNGG | 4 cut(s) | 200, 410, 867, 996 |
| MvaI | CCWGG | 2 cut(s) | 410, 996 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 233, 497 |
| NaeI | GCCGGC | 1 cut(s) | 94 |
| NciI | CCSGG | 2 cut(s) | 200, 867 |
| NcoI | CCATGG | 2 cut(s) | 228, 1009 |
| NdeI | CATATG | 1 cut(s) | 1205 |
| NdeII | GATC | 2 cut(s) | 586, 1210 |
| NgoMIV | GCCGGC | 1 cut(s) | 92 |
| NheI | GCTAGC | 1 cut(s) | 88 |
| NlaIII | CATG | 6 cut(s) | 232, 407, 607, 1013, 1162, 1265 |
| NlaIV | GGNNCC | 7 cut(s) | 226, 354, 588, 717, 859, 1114, 1128 |
| NmuCI | GTSAC | 1 cut(s) | 822 |
| NsiI | ATGCAT | 1 cut(s) | 1222 |
| NspI | RCATGY | 1 cut(s) | 1265 |
| PciI | ACATGT | 1 cut(s) | 1261 |
| PciSI | GCTCTTC | 1 cut(s) | 78 |
| PdiI | GCCGGC | 1 cut(s) | 94 |
| PdmI | GAANNNNTTC | 2 cut(s) | 344, 1111 |
| PfeI | GAWTC | 2 cut(s) | 42, 187 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 810 |
| PkrI | GCNGC | 4 cut(s) | 235, 238, 380, 521 |
| PscI | ACATGT | 1 cut(s) | 1261 |
| Psp6I | CCWGG | 2 cut(s) | 408, 994 |
| PspEI | GGTNACC | 1 cut(s) | 989 |
| PspFI | CCCAGC | 1 cut(s) | 700 |
| PspGI | CCWGG | 2 cut(s) | 408, 994 |
| PspN4I | GGNNCC | 7 cut(s) | 226, 354, 588, 717, 859, 1114, 1128 |
| PspPI | GGNCC | 4 cut(s) | 225, 353, 446, 1180 |
| PstI | CTGCAG | 1 cut(s) | 241 |
| PsuI | RGATCY | 1 cut(s) | 586 |
| PvuII | CAGCTG | 1 cut(s) | 236 |
| RsaI | GTAC | 7 cut(s) | 370, 639, 859, 918, 1007, 1022, 1128 |
| RsaNI | GTAC | 7 cut(s) | 369, 638, 858, 917, 1006, 1021, 1127 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1073, 1266 |
| SapI | GCTCTTC | 1 cut(s) | 78 |
| SaqAI | TTAA | 3 cut(s) | 68, 315, 951 |
| SatI | GCNGC | 4 cut(s) | 234, 237, 379, 520 |
| Sau3AI | GATC | 2 cut(s) | 586, 1210 |
| Sau96I | GGNCC | 4 cut(s) | 225, 353, 446, 1180 |
| ScrFI | CCNGG | 4 cut(s) | 200, 410, 867, 996 |
| SduI | GDGCHC | 4 cut(s) | 200, 470, 531, 603 |
| SfaNI | GCATC | 2 cut(s) | 1206, 1229 |
| SfcI | CTRYAG | 1 cut(s) | 237 |
| SinI | GGWCC | 3 cut(s) | 353, 446, 1180 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1073, 1266 |
| SmlI | CTYRAG | 2 cut(s) | 173, 1149 |
| SmoI | CTYRAG | 2 cut(s) | 173, 1149 |
| SpeI | ACTAGT | 2 cut(s) | 371, 794 |
| Sse9I | AATT | 7 cut(s) | 388, 419, 721, 762, 1083, 1131, 1162 |
| SsiI | CCGC | 7 cut(s) | 107, 475, 740, 770, 827, 1178, 1201 |
| SspMI | CTAG | 7 cut(s) | 89, 372, 494, 726, 795, 1017, 1224 |
| StyD4I | CCNGG | 4 cut(s) | 198, 408, 865, 994 |
| StyI | CCWWGG | 3 cut(s) | 228, 968, 1009 |
| TaaI | ACNGT | 1 cut(s) | 616 |
| TaqI | TCGA | 3 cut(s) | 348, 838, 1213 |
| TasI | AATT | 7 cut(s) | 388, 419, 721, 762, 1083, 1131, 1162 |
| TatI | WGTACW | 1 cut(s) | 916 |
| TfiI | GAWTC | 2 cut(s) | 42, 187 |
| Tru1I | TTAA | 3 cut(s) | 68, 315, 951 |
| Tru9I | TTAA | 3 cut(s) | 68, 315, 951 |
| TscAI | CASTG | 4 cut(s) | 34, 493, 529, 1104 |
| TseFI | GTSAC | 1 cut(s) | 822 |
| TseI | GCWGC | 4 cut(s) | 233, 236, 378, 519 |
| Tsp45I | GTSAC | 1 cut(s) | 822 |
| TspDTI | ATGAA | 5 cut(s) | 66, 116, 264, 1091, 1175 |
| TspRI | CASTG | 4 cut(s) | 34, 493, 529, 1104 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 810 |
| VpaK11BI | GGWCC | 3 cut(s) | 353, 446, 1180 |
| XapI | RAATTY | 3 cut(s) | 388, 419, 721 |
| XbaI | TCTAGA | 1 cut(s) | 1223 |
| XceI | RCATGY | 1 cut(s) | 1265 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 19 |
| XmnI | GAANNNNTTC | 2 cut(s) | 344, 1111 |
| XspI | CTAG | 7 cut(s) | 89, 372, 494, 726, 795, 1017, 1224 |
| Zsp2I | ATGCAT | 1 cut(s) | 1222 |