pycom02g20500

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000385
Physical Location & Seq
Forward (+)
18590425 .. 18592608
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20500.1

Sequence Viewer

Length: 2184 bp
ATGGTGTCCGGAGTCTCCGACCCAAGGGAGCGTACTGCCGAGGTGCAAGATGTTGCCAAGAGCAAGCCAAAGTTAAAGGACTTGCAAGCATCAATGGAGACCATGGAGGAGCGACTCGAGAAGGTGGAACGAACCATACTCGAGTTCGATACTCGACTTGATGAGGACGTTGTCGACAAGGAGGAAATCCAAACCATGGTGGAAGATGGTAAGGATGAACTACGTGGCTTGGTGGAAGGGCTACGAGATGAGCTCCTTGGCGCCCTCAATACCATGGCCGACAAGATGCGGAGGGAAGTCCAGGTTCACCTTGACAACATGGAGGCAAGGCTTGTGGCGCTTCAAGAGGAGGTAAAAGACACAAAAGAACTTAAGGGAGATGTGGCGTTTTGCAAAGAAGCAATTGTGAAACAATTCGTGCAAGGGCCGAGGGAAATCAAGACGTTGGACTCAAAGGTAATCGACTCCTTTAAACCCAAATCCTATAATGGGAAAAGGGAAGCAAAGGAGCTCGACACATTTGTGTGGAACGTGGAGCGATACTTCAAGTACCTGAAGCTTGAAGATGAAGAGTCCAAAATCGCAACGGCAACCATGTTCTTAGCTGACAATGCCCTTATGTGGTGGCGTCGCCGGAGCATGGAGATTGAACAAGGTACGTTCTCCCTCACCACTTGGGATGAATTTAAGAAAGATCTTATGTTGCACTTCTATCCCCAAAATGCCAAGTACGAAGCCAAGGAGAAACTAAGGTGGCTCAAGCAAACGGGGAGTGTCAAAGACTATGTCAACACCTTCGTGAGCTTGTTATTCGAGGTGCCCAACATGTTAGAGGAAGACAAGCTCATGTACTTCATGAGTGGACTGCAAAATTGGGCAAAACTCGAACTACAAAGGAGGCATGTGCAAACGTTGTCTGATGCCATTGCCGCCGCCGAATCCCTGATTGAATTTAGATCAAGCCACCAAGGTGATTCCAAGTCCACGGGGAAGAGGGGTAACCATGAGAAAAGTGGGGGAGCACATAAGCCGAAGGATAAGGCGGAGACAAGCAAAGGGGCTGAGACAAGCAAACCGAAGGAGAAGAAAGCCGATAAGCATGACAAAGGCAAGGGTAAGTCTTGGCAACCCACTTGTTACTTATGCAACGGCCCTCACATGATGCGAGATTGCCCACAAAAGAACGCCCTTAAGGCCATAGTTTTCAAGGAGAACAAGGCTGAGGAGAGTAACGATGCAAGGATGGGATGCATCCGTCTCCTGAATGCCATCCAAACAACCCTTCCACAACCTAAGGCTCAAGTTGAGGGAGGATCATTGTTCGTCGACGTCAAGACTGGTGACAAGACGACGCGTGTGTTGGTGGACACAGGAGCAACACACAACTTCATGACGTCGGAGGAAGCTATAAGGCTTGGCCTCCGAGTCACAAAGGAGCCCGGTAGCGTGAAGACGGTAAATTCCGCTGCCACCCCCATCGTCGGAATTGCGCGCAATGTGCAAGTAGACATTGGCGCATGGAAGGGAAAAATCGACTTCACCATTGTCAAGATGGACGACTATGGCGTAGTCATTGGGCTAGAGTTCATGGACAAGGTACGAGCCTTTCCCATTCCCTTCTACAATATTTTCTGTATCTTGGACGAAGGAAGACAACCTTGCCTGGTGCCATTGGAGAGGCAAGCCAAGAAGTGTATCCAGCACTTGTCGGCAATGCAATTCGCCAAGTCCTGGAAGAAAGGCGAGGCCACATTTCTCGCAACCCTAATGTTGAATGAAGGGGAGGAGAAGTACGGGCCTTTGCCGAAACAAGTGGAAGCAGTCCTTATGGAGTTCGCGGACGTGATGCCTAAGGAACTGCCAAAGAAGTTGCCACCAAGGAGAGAGGTCGACCATGCGATTGAGTTGGAGCCTGGTGCTAAGCCTCCCTCCAAATCACCTTATAGGATGTCGCCACCCGAGTTGGAGGAGTTGAGGAAGCAACTCAACGAGCTACTGGATGCTGGCTACATCCAACCCTCCAAGTCCCCATATGGTGCACCCGTCTTGTTCCAACGCAAGAAAGAAGGTAGCCTAAGGCTGTGCATCGACTATCGAGCATTGAACAAGATTACCATCAAGAACAAGTACCCACTTCCGTTGATCGATCGGCCCGCGAGCCGACCTATTCGATCAACTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Chromo RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

728

Amino Acids

82.79

Weight (kDa)

8.59

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 158 - 318 6.4e-14 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 197 - 290 1.8e-15 Retrotransposon gag protein
gag-asp_proteas PF13975 442 - 531 4.5e-10 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 442 - 529 7e-09 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23713 FvH4_1g25744 FvH4_2g00261 FvH4_2g00262 FvH4_2g15681 FvH4_2g17851 FvH4_2g35550 FvH4_3g05280 FvH4_3g10531 FvH4_3g25910 FvH4_3g28953 FvH4_3g33371 FvH4_3g33536 FvH4_3g36090 FvH4_4g03471 FvH4_4g03480 FvH4_4g06701 FvH4_4g19351 FvH4_4g34281 FvH4_5g00040 FvH4_5g03811 FvH4_5g03812 FvH4_5g11822 FvH4_5g30921 FvH4_5g31701 FvH4_6g12161 FvH4_6g15222 FvH4_6g15223 FvH4_6g17701 FvH4_6g17710 FvH4_6g19301 FvH4_6g33011 FvH4_6g34501 FvH4_6g50161 FvH4_7g04111 FvH4_7g04120 FvH4_7g04121 FvH4_7g04122 FvH4_7g04123 FvH4_7g28231 FvH4_c7g00010
malus_domestica MD12G1084200.v1.1
prunus_persica Prupe.1G206200_v2.0.a1 Prupe.8G085600_v2.0.a1
pyrus_communis pycom01g11080 pycom02g20500 pycom09g18370 pycom12555g00210 pycom13g23120 pycom14g10590 pycom16g17620 pycom16g22750
rosa_chinensis RchiOBHm_Chr2g0137481 RchiOBHm_Chr6g0251961 RchiOBHm_Chr7g0185821
rosa_laevigata RLG00000005585 RLG00000010281 RLG00000010912 RLG00000030342 RLG00000034281
rosa_multiflora Rmu_sc0000096.1_g000010 Rmu_sc0002481.1_g000045 Rmu_sc0003598.1_g000009 Rmu_sc0003863.1_g000019 Rmu_sc0003973.1_g000002 Rmu_sc0004823.1_g000050 Rmu_sc0005621.1_g000007 Rmu_sc0006537.1_g000030 Rmu_sc0006608.1_g000014 Rmu_sc0008506.1_g000007 Rmu_sc0014428.1_g000001 Rmu_sc0035437.1_g000001 Rmu_ssc0000396.1_g000022
rosa_roxburghii Rroxscaffold_175G00432300 Rroxscaffold_17G00435390 Rroxscaffold_27G00446590 Rroxscaffold_29G00441820 Rroxscaffold_31G00438110 Rroxscaffold_32G00442750 Rroxscaffold_33G00439810 Rroxscaffold_37G00445160 Rroxscaffold_4G00306500 Rroxscaffold_4G00327700 Rroxscaffold_4G00332630 Rroxscaffold_52G00439360 Rroxscaffold_63G00444420 Rroxscaffold_76G00448580 Rroxscaffold_7G00156270
rosa_rugosa Rorug03G0295200
rosa_samantha Rh1BG433700 Rh1CG115400 Rh2AG676300 Rh2CG341800 Rh2DG381400 Rh3BG370100 Rh3BG370200 Rh4AG441600 Rh4BG076500 Rh4BG107400 Rh5BG005600 Rh6BG010100 Rh7BG000200
rosa_wichuraiana Rw0G003820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1332, 1397
AccB1I GGYRCC 3 cut(s) 260, 817, 1666
AccB7I CCANNNNNTGG 2 cut(s) 196, 1731
AccI GTMKAC 4 cut(s) 174, 1326, 1506, 1890
AccII CGCG 4 cut(s) 1354, 1492, 1838, 2156
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 7 cut(s) 289, 930, 933, 1043, 1464, 1838, 2154
AclI AACGTT 1 cut(s) 911
AclWI GGATC 1 cut(s) 1321
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 3 cut(s) 683, 950, 1459
AcuI CTGAAG 1 cut(s) 575
AcyI GRCGYC 4 cut(s) 261, 628, 1329, 1394
AfaI GTAC 8 cut(s) 34, 551, 658, 731, 851, 1599, 1793, 2129
AfiI CCNNNNNNNGG 7 cut(s) 24, 196, 489, 621, 640, 1481, 1731
AflII CTTAAG 2 cut(s) 371, 1190
AflIII ACRYGT 2 cut(s) 825, 1352
AgsI TTSAA 8 cut(s) 344, 547, 563, 650, 950, 1207, 1774, 2104
AjiI CACGTC 1 cut(s) 1843
AjnI CCWGG 4 cut(s) 300, 1662, 1730, 1912
AjuI GAANNNNNNNTTGG 2 cut(s) 1266, 1298
AleI CACNNNNGTG 3 cut(s) 521, 797, 969
AluBI AGCT 8 cut(s) 253, 511, 559, 605, 804, 844, 1406, 1993
AluI AGCT 8 cut(s) 253, 511, 559, 605, 804, 844, 1406, 1993
Alw21I GWGCWC 4 cut(s) 255, 513, 1024, 2041
Alw26I GTCTC 5 cut(s) 19, 92, 1040, 1058, 1262
Alw44I GTGCAC 1 cut(s) 2037
AlwI GGATC 1 cut(s) 1321
Ama87I CYCGRG 3 cut(s) 116, 140, 1958
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 8 cut(s) 276, 425, 1150, 1194, 1417, 1746, 1796, 2150
ApaLI GTGCAC 1 cut(s) 2037
ApeKI GCWGC 1 cut(s) 1466
ApoI RAATTY 3 cut(s) 683, 950, 1459
ArsI GACNNNNNNTTYG 2 cut(s) 771, 803
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 5 cut(s) 263, 340, 1492, 1494, 1517
AspS9I GGNCC 4 cut(s) 425, 1151, 1796, 2151
AsuC2I CCSGG 1 cut(s) 1440
AsuHPI GGTGA 6 cut(s) 299, 661, 983, 1352, 1531, 1929
AvaI CYCGRG 3 cut(s) 116, 140, 1958
AxyI CCTNAGG 3 cut(s) 1293, 1851, 2075
BaeGI GKGCMC 2 cut(s) 822, 2041
BanI GGYRCC 3 cut(s) 260, 817, 1666
BanII GRGCYC 3 cut(s) 255, 513, 1440
BbsI GAAGAC 3 cut(s) 843, 1457, 1657
Bbv12I GWGCWC 4 cut(s) 255, 513, 1024, 2041
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 1 cut(s) 1453
BccI CCATC 6 cut(s) 200, 1237, 1277, 1484, 1546, 2123
BceAI ACGGC 2 cut(s) 603, 1165
BciT130I CCWGG 4 cut(s) 302, 1664, 1732, 1914
BciVI GTATCC 1 cut(s) 1706
BcnI CCSGG 1 cut(s) 1440
BcoDI GTCTC 5 cut(s) 19, 92, 1040, 1058, 1262
BfaI CTAG 1 cut(s) 1580
BfoI RGCGCY 2 cut(s) 264, 341
BfrI CTTAAG 2 cut(s) 371, 1190
BfuI GTATCC 1 cut(s) 1706
BglI GCCNNNNNGGC 1 cut(s) 1193
BglII AGATCT 1 cut(s) 694
BisI GCNGC 3 cut(s) 930, 933, 1467
BlpI GCTNAGC 1 cut(s) 1920
BlsI GCNGC 3 cut(s) 931, 934, 1468
Bme1390I CCNGG 5 cut(s) 302, 1440, 1664, 1732, 1914
BmeT110I CYCGRG 3 cut(s) 116, 140, 1958
BmgBI CACGTC 1 cut(s) 1843
BmgT120I GGNCC 4 cut(s) 425, 1151, 1796, 2151
BmiI GGNNCC 5 cut(s) 262, 819, 1437, 1668, 1911
BmrFI CCNGG 5 cut(s) 302, 1440, 1664, 1732, 1914
BpiI GAAGAC 3 cut(s) 843, 1457, 1657
BplI GAGNNNNNCTC 2 cut(s) 237, 269
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu1102I GCTNAGC 1 cut(s) 1920
BpuEI CTTGAG 2 cut(s) 743, 1284
BpuMI CCSGG 1 cut(s) 1440
Bsa29I ATCGAT 1 cut(s) 2145
BsaAI YACGTR 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 2114
BsaHI GRCGYC 4 cut(s) 261, 628, 1329, 1394
BsaI GGTCTC 1 cut(s) 92
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 1775, 1805
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 196, 489, 621, 640, 1481, 1731
Bse1I ACTGG 2 cut(s) 1342, 2001
Bse21I CCTNAGG 3 cut(s) 1293, 1851, 2075
Bse3DI GCAATG 3 cut(s) 924, 1501, 1719
Bse8I GATNNNNATC 1 cut(s) 2114
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 4 cut(s) 302, 1664, 1732, 1914
BseCI ATCGAT 1 cut(s) 2145
BseGI GGATG 9 cut(s) 220, 685, 1248, 1251, 1253, 1269, 1953, 2005, 2010
BseJI GATNNNNATC 1 cut(s) 2114
BseLI CCNNNNNNNGG 7 cut(s) 24, 196, 489, 621, 640, 1481, 1731
BseMI GCAATG 3 cut(s) 924, 1501, 1719
BseMII CTCAG 2 cut(s) 1053, 1212
BseNI ACTGG 2 cut(s) 1342, 2001
BsePI GCGCGC 1 cut(s) 1490
BseRI GAGGAG 5 cut(s) 122, 362, 1238, 1799, 1982
BseSI GKGCMC 2 cut(s) 822, 2041
BseXI GCAGC 1 cut(s) 1453
Bsh1236I CGCG 4 cut(s) 1354, 1492, 1838, 2156
Bsh1285I CGRYCG 1 cut(s) 2149
BshFI GGCC 8 cut(s) 278, 427, 1152, 1196, 1419, 1748, 1798, 2152
BshNI GGYRCC 3 cut(s) 260, 817, 1666
BshVI ATCGAT 1 cut(s) 2145
BsiEI CGRYCG 1 cut(s) 2149
BsiHKAI GWGCWC 4 cut(s) 255, 513, 1024, 2041
BsiHKCI CYCGRG 3 cut(s) 116, 140, 1958
BsiSI CCGG 3 cut(s) 9, 634, 1440
BslFI GGGAC 1 cut(s) 2011
BslI CCNNNNNNNGG 7 cut(s) 24, 196, 489, 621, 640, 1481, 1731
BsmAI GTCTC 5 cut(s) 19, 92, 1040, 1058, 1262
BsmBI CGTCTC 1 cut(s) 1262
BsmFI GGGAC 1 cut(s) 2011
BsmI GAATGC 1 cut(s) 1270
BsnI GGCC 8 cut(s) 278, 427, 1152, 1196, 1419, 1748, 1798, 2152
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 3 cut(s) 116, 140, 1958
Bsp1286I GDGCHC 6 cut(s) 255, 513, 822, 1024, 1440, 2041
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
Bsp1720I GCTNAGC 1 cut(s) 1920
Bsp19I CCATGG 3 cut(s) 102, 195, 273
BspACI CCGC 7 cut(s) 289, 930, 933, 1043, 1464, 1838, 2154
BspANI GGCC 8 cut(s) 278, 427, 1152, 1196, 1419, 1748, 1798, 2152
BspCNI CTCAG 2 cut(s) 1054, 1213
BspDI ATCGAT 1 cut(s) 2145
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 4 cut(s) 1354, 1492, 1838, 2156
BspHI TCATGA 2 cut(s) 855, 1389
BspLI GGNNCC 5 cut(s) 262, 819, 1437, 1668, 1911
BspPI GGATC 1 cut(s) 1321
BspT107I GGYRCC 3 cut(s) 260, 817, 1666
BspTI CTTAAG 2 cut(s) 371, 1190
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 3 cut(s) 924, 1501, 1719
BsrI ACTGG 2 cut(s) 1342, 2001
BssHII GCGCGC 1 cut(s) 1490
BssMI GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
BssNI GRCGYC 4 cut(s) 261, 628, 1329, 1394
BssT1I CCWWGG 8 cut(s) 23, 102, 195, 256, 273, 738, 967, 1877
Bst2UI CCWGG 4 cut(s) 302, 1664, 1732, 1914
Bst4CI ACNGT 1 cut(s) 1456
Bst6I CTCTTC 2 cut(s) 564, 986
BstACI GRCGYC 4 cut(s) 261, 628, 1329, 1394
BstAFI CTTAAG 2 cut(s) 371, 1190
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 7 cut(s) 65, 87, 1492, 1683, 2005, 2154, 2158
BstDEI CTNAG 8 cut(s) 601, 749, 1062, 1221, 1293, 1851, 1920, 2075
BstDSI CCRYGG 4 cut(s) 102, 195, 273, 984
BstEII GGTNACC 1 cut(s) 998
BstF5I GGATG 9 cut(s) 220, 685, 1248, 1251, 1253, 1269, 1953, 2005, 2010
BstFNI CGCG 4 cut(s) 1354, 1492, 1838, 2156
BstH2I RGCGCY 2 cut(s) 264, 341
BstHHI GCGC 5 cut(s) 263, 340, 1492, 1494, 1517
BstKTI GATC 6 cut(s) 697, 959, 1316, 2145, 2149, 2174
BstMAI GTCTC 5 cut(s) 19, 92, 1040, 1058, 1262
BstMBI GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
BstMCI CGRYCG 1 cut(s) 2149
BstMWI GCNNNNNNNGC 5 cut(s) 337, 611, 929, 1193, 1498
BstNI CCWGG 4 cut(s) 302, 1664, 1732, 1914
BstNSI RCATGY 2 cut(s) 829, 905
BstPI GGTNACC 1 cut(s) 998
BstSCI CCNGG 5 cut(s) 300, 1438, 1662, 1730, 1912
BstSLI GKGCMC 2 cut(s) 822, 2041
BstUI CGCG 4 cut(s) 1354, 1492, 1838, 2156
BstV1I GCAGC 1 cut(s) 1453
BstV2I GAAGAC 3 cut(s) 843, 1457, 1657
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
Bsu15I ATCGAT 1 cut(s) 2145
Bsu36I CCTNAGG 3 cut(s) 1293, 1851, 2075
BsuI GTATCC 1 cut(s) 1706
BsuRI GGCC 8 cut(s) 278, 427, 1152, 1196, 1419, 1748, 1798, 2152
BsuTUI ATCGAT 1 cut(s) 2145
BtgI CCRYGG 4 cut(s) 102, 195, 273, 984
BtrI CACGTC 1 cut(s) 1843
BtsCI GGATG 9 cut(s) 220, 685, 1248, 1251, 1253, 1269, 1953, 2005, 2010
Cac8I GCNNGC 7 cut(s) 65, 87, 1492, 1683, 2005, 2154, 2158
CciI TCATGA 2 cut(s) 855, 1389
CfoI GCGC 5 cut(s) 263, 340, 1492, 1494, 1517
Cfr13I GGNCC 4 cut(s) 425, 1151, 1796, 2151
ClaI ATCGAT 1 cut(s) 2145
CseI GACGC 2 cut(s) 617, 1360
Csp6I GTAC 8 cut(s) 33, 550, 657, 730, 850, 1598, 1792, 2128
CspCI CAANNNNNGTGG 6 cut(s) 315, 350, 1944, 1979, 2121, 2156
CviQI GTAC 8 cut(s) 33, 550, 657, 730, 850, 1598, 1792, 2128
DdeI CTNAG 8 cut(s) 601, 749, 1062, 1221, 1293, 1851, 1920, 2075
DinI GGCGCC 1 cut(s) 262
DpnI GATC 6 cut(s) 696, 958, 1315, 2144, 2148, 2173
DpnII GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
DraI TTTAAA 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 564, 986
EarI CTCTTC 2 cut(s) 564, 986
EciI GGCGGA 1 cut(s) 1058
Ecl136II GAGCTC 2 cut(s) 253, 511
Eco130I CCWWGG 8 cut(s) 23, 102, 195, 256, 273, 738, 967, 1877
Eco24I GRGCYC 3 cut(s) 255, 513, 1440
Eco31I GGTCTC 1 cut(s) 92
Eco53kI GAGCTC 2 cut(s) 253, 511
Eco57I CTGAAG 1 cut(s) 575
Eco81I CCTNAGG 3 cut(s) 1293, 1851, 2075
Eco88I CYCGRG 3 cut(s) 116, 140, 1958
Eco91I GGTNACC 1 cut(s) 998
EcoICRI GAGCTC 2 cut(s) 253, 511
EcoO65I GGTNACC 1 cut(s) 998
EcoRII CCWGG 4 cut(s) 300, 1662, 1730, 1912
EcoT14I CCWWGG 8 cut(s) 23, 102, 195, 256, 273, 738, 967, 1877
EcoT22I ATGCAT 1 cut(s) 1253
EcoT38I GRGCYC 3 cut(s) 255, 513, 1440
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 8 cut(s) 23, 102, 195, 256, 273, 738, 967, 1877
Esp3I CGTCTC 1 cut(s) 1262
FalI AAGNNNNNCTT 4 cut(s) 1642, 1674, 1809, 1841
FaqI GGGAC 1 cut(s) 2011
FauI CCCGC 1 cut(s) 2161
FauNDI CATATG 1 cut(s) 2032
FblI GTMKAC 4 cut(s) 174, 1326, 1506, 1890
Fnu4HI GCNGC 3 cut(s) 930, 933, 1467
FokI GGATG 9 cut(s) 227, 692, 1238, 1255, 1256, 1260, 1960, 1997, 2012
FriOI GRGCYC 3 cut(s) 255, 513, 1440
Fsp4HI GCNGC 3 cut(s) 930, 933, 1467
FspBI CTAG 1 cut(s) 1580
GlaI GCGC 5 cut(s) 262, 339, 1491, 1493, 1516
GluI GCNGC 3 cut(s) 930, 933, 1467
HaeII RGCGCY 2 cut(s) 264, 341
HaeIII GGCC 8 cut(s) 278, 427, 1152, 1196, 1419, 1748, 1798, 2152
HapII CCGG 3 cut(s) 9, 634, 1440
HgaI GACGC 2 cut(s) 617, 1360
HhaI GCGC 5 cut(s) 263, 340, 1492, 1494, 1517
Hin1I GRCGYC 4 cut(s) 261, 628, 1329, 1394
Hin6I GCGC 5 cut(s) 261, 338, 1490, 1492, 1515
HinP1I GCGC 5 cut(s) 261, 338, 1490, 1492, 1515
HincII GTYRAC 4 cut(s) 175, 790, 1327, 1891
HindII GTYRAC 4 cut(s) 175, 790, 1327, 1891
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 8 cut(s) 12, 114, 449, 464, 572, 938, 974, 1425
HpaII CCGG 3 cut(s) 9, 634, 1440
HphI GGTGA 6 cut(s) 299, 661, 983, 1352, 1531, 1929
Hpy188I TCNGA 5 cut(s) 19, 919, 1399, 1424, 1484
Hpy99I CGWCG 6 cut(s) 633, 1328, 1331, 1354, 1399, 1484
HpyCH4III ACNGT 1 cut(s) 1456
HpyCH4IV ACGT 9 cut(s) 168, 223, 443, 531, 659, 911, 1329, 1394, 1842
HpyF10VI GCNNNNNNNGC 5 cut(s) 337, 611, 929, 1193, 1498
HpyF3I CTNAG 8 cut(s) 601, 749, 1062, 1221, 1293, 1851, 1920, 2075
HpySE526I ACGT 9 cut(s) 168, 223, 443, 531, 659, 911, 1329, 1394, 1842
Hsp92I GRCGYC 4 cut(s) 261, 628, 1329, 1394
HspAI GCGC 5 cut(s) 261, 338, 1490, 1492, 1515
KasI GGCGCC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 8
Kzo9I GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
Lsp1109I GCAGC 1 cut(s) 1453
MaeI CTAG 1 cut(s) 1580
MaeII ACGT 9 cut(s) 168, 223, 443, 531, 659, 911, 1329, 1394, 1842
MaeIII GTNAC 5 cut(s) 998, 1136, 1229, 1340, 1426
MalI GATC 6 cut(s) 696, 958, 1315, 2144, 2148, 2173
MboI GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
MboII GAAGA 9 cut(s) 215, 575, 581, 848, 1003, 1096, 1462, 1662, 1747
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 6 cut(s) 255, 513, 822, 1024, 1440, 2041
MluCI AATT 8 cut(s) 402, 413, 683, 871, 950, 1459, 1485, 1718
MluI ACGCGT 1 cut(s) 1352
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 6 cut(s) 21, 108, 443, 458, 581, 1434
MmeI TCCRAC 8 cut(s) 42, 426, 1377, 1462, 1887, 1944, 2038, 2077
Mph1103I ATGCAT 1 cut(s) 1253
MroI TCCGGA 1 cut(s) 8
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 5 cut(s) 74, 372, 471, 687, 1191
MslI CAYNNNNRTG 4 cut(s) 521, 523, 797, 969
MspA1I CMGCKG 1 cut(s) 1466
MspCI CTTAAG 2 cut(s) 371, 1190
MspI CCGG 3 cut(s) 9, 634, 1440
MspR9I CCNGG 5 cut(s) 302, 1440, 1664, 1732, 1914
MunI CAATTG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 1270
MvaI CCWGG 4 cut(s) 302, 1664, 1732, 1914
MvnI CGCG 4 cut(s) 1354, 1492, 1838, 2156
MwoI GCNNNNNNNGC 5 cut(s) 337, 611, 929, 1193, 1498
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 1 cut(s) 1440
NcoI CCATGG 3 cut(s) 102, 195, 273
NdeI CATATG 1 cut(s) 2032
NdeII GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
NlaIV GGNNCC 5 cut(s) 262, 819, 1437, 1668, 1911
NmeAIII GCCGAG 2 cut(s) 64, 453
NmuCI GTSAC 2 cut(s) 1340, 1426
NsiI ATGCAT 1 cut(s) 1253
NspI RCATGY 2 cut(s) 829, 905
OliI CACNNNNGTG 3 cut(s) 521, 797, 969
PaeR7I CTCGAG 2 cut(s) 116, 140
PagI TCATGA 2 cut(s) 855, 1389
PauI GCGCGC 1 cut(s) 1490
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 1563
PctI GAATGC 1 cut(s) 1270
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 938, 974
PflFI GACNNNGTC 2 cut(s) 170, 785
PflMI CCANNNNNTGG 2 cut(s) 196, 1731
PfoI TCCNGGA 1 cut(s) 1730
PkrI GCNGC 3 cut(s) 931, 934, 1468
Ple19I CGATCG 1 cut(s) 2149
PleI GAGTC 6 cut(s) 20, 108, 443, 458, 580, 1433
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 6 cut(s) 20, 108, 443, 458, 580, 1433
Ppu21I YACGTR 1 cut(s) 224
PscI ACATGT 1 cut(s) 825
Psp124BI GAGCTC 2 cut(s) 255, 513
Psp1406I AACGTT 1 cut(s) 911
Psp6I CCWGG 4 cut(s) 300, 1662, 1730, 1912
PspEI GGTNACC 1 cut(s) 998
PspGI CCWGG 4 cut(s) 300, 1662, 1730, 1912
PspN4I GGNNCC 5 cut(s) 262, 819, 1437, 1668, 1911
PspPI GGNCC 4 cut(s) 425, 1151, 1796, 2151
PspXI VCTCGAGB 1 cut(s) 140
PsrI GAACNNNNNNTAC 2 cut(s) 120, 152
PsuI RGATCY 1 cut(s) 694
PsyI GACNNNGTC 2 cut(s) 170, 785
PteI GCGCGC 1 cut(s) 1490
PvuI CGATCG 1 cut(s) 2149
RsaI GTAC 8 cut(s) 34, 551, 658, 731, 851, 1599, 1793, 2129
RsaNI GTAC 8 cut(s) 33, 550, 657, 730, 850, 1598, 1792, 2128
RseI CAYNNNNRTG 4 cut(s) 521, 523, 797, 969
SacI GAGCTC 2 cut(s) 255, 513
SalI GTCGAC 3 cut(s) 173, 1325, 1889
SaqAI TTAA 5 cut(s) 74, 372, 471, 687, 1191
SatI GCNGC 3 cut(s) 930, 933, 1467
Sau3AI GATC 6 cut(s) 694, 956, 1313, 2142, 2146, 2171
Sau96I GGNCC 4 cut(s) 425, 1151, 1796, 2151
SchI GAGTC 6 cut(s) 21, 108, 443, 458, 581, 1434
ScrFI CCNGG 5 cut(s) 302, 1440, 1664, 1732, 1914
SduI GDGCHC 6 cut(s) 255, 513, 822, 1024, 1440, 2041
SfoI GGCGCC 1 cut(s) 262
Sfr274I CTCGAG 2 cut(s) 116, 140
SgrDI CGTCGACG 1 cut(s) 1325
SlaI CTCGAG 2 cut(s) 116, 140
SmiMI CAYNNNNRTG 4 cut(s) 521, 523, 797, 969
SmlI CTYRAG 6 cut(s) 116, 140, 371, 758, 1190, 1299
SmoI CTYRAG 6 cut(s) 116, 140, 371, 758, 1190, 1299
Sse9I AATT 8 cut(s) 402, 413, 683, 871, 950, 1459, 1485, 1718
SsiI CCGC 7 cut(s) 289, 930, 933, 1043, 1464, 1838, 2154
SspDI GGCGCC 1 cut(s) 260
SspI AATATT 1 cut(s) 1627
SspMI CTAG 1 cut(s) 1580
SstI GAGCTC 2 cut(s) 255, 513
StyD4I CCNGG 5 cut(s) 300, 1438, 1662, 1730, 1912
StyI CCWWGG 8 cut(s) 23, 102, 195, 256, 273, 738, 967, 1877
TaaI ACNGT 1 cut(s) 1456
TaiI ACGT 9 cut(s) 171, 226, 446, 534, 662, 914, 1332, 1397, 1845
TasI AATT 8 cut(s) 402, 413, 683, 871, 950, 1459, 1485, 1718
TatI WGTACW 1 cut(s) 849
TauI GCSGC 2 cut(s) 932, 935
TfiI GAWTC 2 cut(s) 938, 974
Tru1I TTAA 5 cut(s) 74, 372, 471, 687, 1191
Tru9I TTAA 5 cut(s) 74, 372, 471, 687, 1191
TseFI GTSAC 2 cut(s) 1340, 1426
TseI GCWGC 1 cut(s) 1466
Tsp45I GTSAC 2 cut(s) 1340, 1426
TspDTI ATGAA 7 cut(s) 231, 582, 696, 844, 1378, 1576, 1791
TspGWI ACGGA 2 cut(s) 1244, 2127
Tth111I GACNNNGTC 2 cut(s) 170, 785
Van91I CCANNNNNTGG 2 cut(s) 196, 1731
Vha464I CTTAAG 2 cut(s) 371, 1190
VneI GTGCAC 1 cut(s) 2037
XapI RAATTY 3 cut(s) 683, 950, 1459
XceI RCATGY 2 cut(s) 829, 905
XcmI CCANNNNNNNNNTGG 2 cut(s) 1010, 1549
XhoI CTCGAG 2 cut(s) 116, 140
XmiI GTMKAC 4 cut(s) 174, 1326, 1506, 1890
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 1 cut(s) 1580
ZraI GACGTC 2 cut(s) 1330, 1395
Zsp2I ATGCAT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.