pycom12555g00210

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012555
OG0000385
Physical Location & Seq
Forward (+)
113428 .. 114243
816 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12555g00210.1

Sequence Viewer

Length: 816 bp
ATGATGCGAGATTGCCCACAAAAGAACGCCCTTAAGGCCATAGTTTTCAAGGAGAACAAGGCTGAGGAGAGTAACGATGCAAGGATGGGATGCATCCGTCTCCTGAATGCCATCCAAACAACCCTTCCACAACCTAAGGCTCAAGTTGAGGGAGGATCATTGTTCGTCGACGTCAAGACTGGTGACAAGACGACGCGTGTGTTGGTGGACACGGGAGCAACACACAACTTCATGACGTCGGAGGAAGCTATAAGGCTTGGCCTCCGAGTCACAAAGGAGCCCGGTAGCGTGAAGACGGTAAATTCCGCTGCCACCCCCATCGTCGGAATTGCGCGCAATGTGCAAGTAGACATTGGCGCATGGAAGGGAAAAATCGACTTCACCATTGTCAAGATGGACGACTATGGCGTAGTCATTGGATTAGAGTTCATGGACAAGGTACGAGCCTTTCCCATTCCCTTCTACAATATTTTCTGTATCTTGGACGAAGGAAGACAACCTTGCTTGGTGCCATTGGAGAGGCAAGCCAAGAAGGGTACCCAGCACTTGTCTGCAATGCAATTTGCCAAGTCCTGGAAGAAAGGCGAGGCCACATTTCTTGCAACCCTAATGTTGAATGAAGGGGAGGAGAAGTACGGGCCTTTGCCGAAACAAGTGGAAGCAGTCCTTATGGAGTTCGCGGACGTGATGCCTAAGGAACTGCCAAAGAAGTTGCCACCAAGGAGAGAGGTCGACCATGCGATTGAGTTGGAGCCTGGTGCTAAGCCTCCCTCCAAATCACCTTATAGGATGTCGCCACCCGAGTTGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Chromo RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

272

Amino Acids

30.1

Weight (kDa)

8.23

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag-asp_proteas PF13975 56 - 145 8.6e-11 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 56 - 143 1.4e-09 Aspartyl protease
RVP_2 PF08284 65 - 149 7.2e-06 Retroviral aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23713 FvH4_1g25744 FvH4_2g00261 FvH4_2g00262 FvH4_2g15681 FvH4_2g17851 FvH4_2g35550 FvH4_3g05280 FvH4_3g10531 FvH4_3g25910 FvH4_3g28953 FvH4_3g33371 FvH4_3g33536 FvH4_3g36090 FvH4_4g03471 FvH4_4g03480 FvH4_4g06701 FvH4_4g19351 FvH4_4g34281 FvH4_5g00040 FvH4_5g03811 FvH4_5g03812 FvH4_5g11822 FvH4_5g30921 FvH4_5g31701 FvH4_6g12161 FvH4_6g15222 FvH4_6g15223 FvH4_6g17701 FvH4_6g17710 FvH4_6g19301 FvH4_6g33011 FvH4_6g34501 FvH4_6g50161 FvH4_7g04111 FvH4_7g04120 FvH4_7g04121 FvH4_7g04122 FvH4_7g04123 FvH4_7g28231 FvH4_c7g00010
malus_domestica MD12G1084200.v1.1
prunus_persica Prupe.1G206200_v2.0.a1 Prupe.8G085600_v2.0.a1
pyrus_communis pycom01g11080 pycom02g20500 pycom09g18370 pycom12555g00210 pycom13g23120 pycom14g10590 pycom16g17620 pycom16g22750
rosa_chinensis RchiOBHm_Chr2g0137481 RchiOBHm_Chr6g0251961 RchiOBHm_Chr7g0185821
rosa_laevigata RLG00000005585 RLG00000010281 RLG00000010912 RLG00000030342 RLG00000034281
rosa_multiflora Rmu_sc0000096.1_g000010 Rmu_sc0002481.1_g000045 Rmu_sc0003598.1_g000009 Rmu_sc0003863.1_g000019 Rmu_sc0003973.1_g000002 Rmu_sc0004823.1_g000050 Rmu_sc0005621.1_g000007 Rmu_sc0006537.1_g000030 Rmu_sc0006608.1_g000014 Rmu_sc0008506.1_g000007 Rmu_sc0014428.1_g000001 Rmu_sc0035437.1_g000001 Rmu_ssc0000396.1_g000022
rosa_roxburghii Rroxscaffold_175G00432300 Rroxscaffold_17G00435390 Rroxscaffold_27G00446590 Rroxscaffold_29G00441820 Rroxscaffold_31G00438110 Rroxscaffold_32G00442750 Rroxscaffold_33G00439810 Rroxscaffold_37G00445160 Rroxscaffold_4G00306500 Rroxscaffold_4G00327700 Rroxscaffold_4G00332630 Rroxscaffold_52G00439360 Rroxscaffold_63G00444420 Rroxscaffold_76G00448580 Rroxscaffold_7G00156270
rosa_rugosa Rorug03G0295200
rosa_samantha Rh1BG433700 Rh1CG115400 Rh2AG676300 Rh2CG341800 Rh2DG381400 Rh3BG370100 Rh3BG370200 Rh4AG441600 Rh4BG076500 Rh4BG107400 Rh5BG005600 Rh6BG010100 Rh7BG000200
rosa_wichuraiana Rw0G003820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 174, 239
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 2 cut(s) 508, 536
AccB7I CCANNNNNTGG 1 cut(s) 573
AccI GTMKAC 3 cut(s) 168, 348, 732
AccII CGCG 3 cut(s) 196, 334, 680
AciI CCGC 2 cut(s) 306, 680
AclWI GGATC 1 cut(s) 163
AcsI RAATTY 1 cut(s) 301
AcyI GRCGYC 2 cut(s) 171, 236
AfaI GTAC 3 cut(s) 441, 538, 635
AfiI CCNNNNNNNGG 2 cut(s) 323, 573
AflII CTTAAG 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 2 cut(s) 49, 616
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 2 cut(s) 572, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 1 cut(s) 248
AluI AGCT 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 4 cut(s) 36, 259, 588, 638
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 1 cut(s) 301
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 3 cut(s) 334, 336, 359
AspS9I GGNCC 1 cut(s) 638
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 3 cut(s) 194, 373, 771
AvaI CYCGRG 1 cut(s) 800
AxyI CCTNAGG 2 cut(s) 135, 693
BanI GGYRCC 2 cut(s) 508, 536
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 2 cut(s) 299, 499
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 295
BccI CCATC 4 cut(s) 79, 119, 326, 388
BciT130I CCWGG 2 cut(s) 574, 756
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 32
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 1 cut(s) 309
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 3 cut(s) 282, 574, 756
BmeT110I CYCGRG 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 638
BmiI GGNNCC 4 cut(s) 279, 510, 538, 753
BmrFI CCNGG 3 cut(s) 282, 574, 756
BmsI GCATC 4 cut(s) 67, 80, 102, 678
BpiI GAAGAC 2 cut(s) 299, 499
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 282
BsaHI GRCGYC 2 cut(s) 171, 236
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 573
Bse1I ACTGG 1 cut(s) 184
Bse21I CCTNAGG 2 cut(s) 135, 693
Bse3DI GCAATG 2 cut(s) 343, 561
BseBI CCWGG 2 cut(s) 574, 756
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 5 cut(s) 90, 93, 95, 111, 795
BseLI CCNNNNNNNGG 2 cut(s) 323, 573
BseMI GCAATG 2 cut(s) 343, 561
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 184
BsePI GCGCGC 1 cut(s) 332
BseRI GAGGAG 2 cut(s) 80, 641
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 540
Bsh1236I CGCG 3 cut(s) 196, 334, 680
BshFI GGCC 4 cut(s) 38, 261, 590, 640
BshNI GGYRCC 2 cut(s) 508, 536
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 323, 573
BsmAI GTCTC 1 cut(s) 104
BsmBI CGTCTC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 4 cut(s) 38, 261, 590, 640
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 155
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 2 cut(s) 306, 680
BspANI GGCC 4 cut(s) 38, 261, 590, 640
BspCNI CTCAG 1 cut(s) 55
BspFNI CGCG 3 cut(s) 196, 334, 680
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 4 cut(s) 279, 510, 538, 753
BspPI GGATC 1 cut(s) 163
BspT107I GGYRCC 2 cut(s) 508, 536
BspTI CTTAAG 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 343, 561
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 719
BssHII GCGCGC 1 cut(s) 332
BssMI GATC 1 cut(s) 155
BssNI GRCGYC 2 cut(s) 171, 236
BssT1I CCWWGG 1 cut(s) 719
Bst2UI CCWGG 2 cut(s) 574, 756
Bst4CI ACNGT 1 cut(s) 298
BstACI GRCGYC 2 cut(s) 171, 236
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 334, 525
BstDEI CTNAG 4 cut(s) 63, 135, 693, 762
BstF5I GGATG 5 cut(s) 90, 93, 95, 111, 795
BstFNI CGCG 3 cut(s) 196, 334, 680
BstHHI GCGC 3 cut(s) 334, 336, 359
BstKTI GATC 1 cut(s) 158
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 35, 340
BstNI CCWGG 2 cut(s) 574, 756
BstSCI CCNGG 3 cut(s) 280, 572, 754
BstUI CGCG 3 cut(s) 196, 334, 680
BstV1I GCAGC 1 cut(s) 295
BstV2I GAAGAC 2 cut(s) 299, 499
Bsu36I CCTNAGG 2 cut(s) 135, 693
BsuRI GGCC 4 cut(s) 38, 261, 590, 640
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 5 cut(s) 90, 93, 95, 111, 795
Cac8I GCNNGC 2 cut(s) 334, 525
CciI TCATGA 1 cut(s) 231
CfoI GCGC 3 cut(s) 334, 336, 359
Cfr13I GGNCC 1 cut(s) 638
CseI GACGC 1 cut(s) 202
Csp6I GTAC 3 cut(s) 440, 537, 634
CspCI CAANNNNNGTGG 3 cut(s) 580, 615, 786
CviAII CATG 4 cut(s) 232, 360, 430, 737
CviQI GTAC 3 cut(s) 440, 537, 634
DdeI CTNAG 4 cut(s) 63, 135, 693, 762
DpnI GATC 1 cut(s) 157
DpnII GATC 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 282
Eco81I CCTNAGG 2 cut(s) 135, 693
Eco88I CYCGRG 1 cut(s) 800
EcoRII CCWGG 2 cut(s) 572, 754
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 719
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 4 cut(s) 235, 363, 433, 740
FaiI YATR 9 cut(s) 41, 233, 251, 361, 405, 431, 671, 738, 786
FalI AAGNNNNNCTT 4 cut(s) 484, 516, 651, 683
FatI CATG 4 cut(s) 231, 359, 429, 736
FblI GTMKAC 3 cut(s) 168, 348, 732
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 5 cut(s) 80, 97, 98, 102, 802
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 309
GlaI GCGC 3 cut(s) 333, 335, 358
GluI GCNGC 1 cut(s) 309
GsaI CCCAGC 1 cut(s) 544
HaeIII GGCC 4 cut(s) 38, 261, 590, 640
HapII CCGG 1 cut(s) 282
HgaI GACGC 1 cut(s) 202
HhaI GCGC 3 cut(s) 334, 336, 359
Hin1I GRCGYC 2 cut(s) 171, 236
Hin1II CATG 4 cut(s) 235, 363, 433, 740
Hin6I GCGC 3 cut(s) 332, 334, 357
HinP1I GCGC 3 cut(s) 332, 334, 357
HincII GTYRAC 2 cut(s) 169, 733
HindII GTYRAC 2 cut(s) 169, 733
HinfI GANTC 1 cut(s) 267
HpaII CCGG 1 cut(s) 282
HphI GGTGA 3 cut(s) 194, 373, 771
Hpy166II GTNNAC 4 cut(s) 169, 208, 349, 733
Hpy188I TCNGA 3 cut(s) 241, 266, 326
Hpy188III TCNNGA 4 cut(s) 103, 175, 232, 391
Hpy8I GTNNAC 4 cut(s) 169, 208, 349, 733
Hpy99I CGWCG 5 cut(s) 170, 173, 196, 241, 326
HpyAV CCTTC 6 cut(s) 134, 358, 469, 482, 526, 614
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 3 cut(s) 171, 236, 684
HpyCH4V TGCA 6 cut(s) 80, 93, 343, 554, 559, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 340
HpyF3I CTNAG 4 cut(s) 63, 135, 693, 762
HpySE526I ACGT 3 cut(s) 171, 236, 684
Hsp92I GRCGYC 2 cut(s) 171, 236
Hsp92II CATG 4 cut(s) 235, 363, 433, 740
HspAI GCGC 3 cut(s) 332, 334, 357
KpnI GGTACC 1 cut(s) 540
Kzo9I GATC 1 cut(s) 155
LmnI GCTCC 3 cut(s) 215, 277, 751
LpnPI CCDG 8 cut(s) 116, 165, 295, 554, 559, 586, 741, 768
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 4 cut(s) 67, 80, 102, 678
MaeII ACGT 3 cut(s) 171, 236, 684
MaeIII GTNAC 3 cut(s) 71, 182, 268
MalI GATC 1 cut(s) 157
MboI GATC 1 cut(s) 155
MboII GAAGA 3 cut(s) 304, 504, 589
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 3 cut(s) 301, 327, 560
MluI ACGCGT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 4 cut(s) 219, 304, 729, 786
Mph1103I ATGCAT 1 cut(s) 95
MseI TTAA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 308
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 3 cut(s) 282, 574, 756
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 2 cut(s) 574, 756
MvnI CGCG 3 cut(s) 196, 334, 680
MwoI GCNNNNNNNGC 2 cut(s) 35, 340
NciI CCSGG 1 cut(s) 282
NdeII GATC 1 cut(s) 155
NlaIII CATG 4 cut(s) 235, 363, 433, 740
NlaIV GGNNCC 4 cut(s) 279, 510, 538, 753
NmuCI GTSAC 2 cut(s) 182, 268
NsiI ATGCAT 1 cut(s) 95
PagI TCATGA 1 cut(s) 231
PauI GCGCGC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 573
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 572, 754
PspFI CCCAGC 1 cut(s) 540
PspGI CCWGG 2 cut(s) 572, 754
PspN4I GGNNCC 4 cut(s) 279, 510, 538, 753
PspPI GGNCC 1 cut(s) 638
PteI GCGCGC 1 cut(s) 332
RsaI GTAC 3 cut(s) 441, 538, 635
RsaNI GTAC 3 cut(s) 440, 537, 634
SalI GTCGAC 2 cut(s) 167, 731
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 1 cut(s) 155
Sau96I GGNCC 1 cut(s) 638
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 3 cut(s) 282, 574, 756
SduI GDGCHC 1 cut(s) 282
SetI ASST 9 cut(s) 136, 174, 239, 250, 441, 502, 687, 732, 784
SfaNI GCATC 4 cut(s) 67, 80, 102, 678
SgrDI CGTCGACG 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 32, 141
SmoI CTYRAG 2 cut(s) 32, 141
Sse9I AATT 3 cut(s) 301, 327, 560
SsiI CCGC 2 cut(s) 306, 680
SspI AATATT 1 cut(s) 469
StyD4I CCNGG 3 cut(s) 280, 572, 754
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 3 cut(s) 174, 239, 687
TaqI TCGA 3 cut(s) 168, 375, 732
TasI AATT 3 cut(s) 301, 327, 560
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 2 cut(s) 182, 268
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 2 cut(s) 182, 268
TspDTI ATGAA 3 cut(s) 220, 418, 633
TspGWI ACGGA 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 573
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 3 cut(s) 168, 348, 732
ZraI GACGTC 2 cut(s) 172, 237
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.