FvH4_5g03812

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000385
Physical Location & Seq
Forward (+)
2272200 .. 2272535
336 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03812.t1

Sequence Viewer

Length: 336 bp
ATGATGCGCGATTACCCTCAAAAGAAAGCATTCATAGCCACGACCTTGCAAGAGCCTAGGATCGAGGCAGAGGAAGATGATGCAGCAAGGATGGGATGCATCCGGCTGCTCAATGCCGTGAAGGCTAGCATCCCTCAACCAAGGCGACAAGCGGAAGGTGGATCGCTTTATATCGATGTCAAGGCAAAAGACACGATGATTCGTGCCTTGGTGGATTGCGGAACAACTCATAACTTCATGACGTCGGAAGAAGCAAGGAAACTTGGCCTCCGAGTCACGAAGGAGTCGGGCAGCGTGAAGACGGTGAATACACCTGCCACACCTATTGTGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

112

Amino Acids

12.21

Weight (kDa)

9.03

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_protease_2 PF13650 59 - 106 3.9e-06 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23713 FvH4_1g25744 FvH4_2g00261 FvH4_2g00262 FvH4_2g15681 FvH4_2g17851 FvH4_2g35550 FvH4_3g05280 FvH4_3g10531 FvH4_3g25910 FvH4_3g28953 FvH4_3g33371 FvH4_3g33536 FvH4_3g36090 FvH4_4g03471 FvH4_4g03480 FvH4_4g06701 FvH4_4g19351 FvH4_4g34281 FvH4_5g00040 FvH4_5g03811 FvH4_5g03812 FvH4_5g11822 FvH4_5g30921 FvH4_5g31701 FvH4_6g12161 FvH4_6g15222 FvH4_6g15223 FvH4_6g17701 FvH4_6g17710 FvH4_6g19301 FvH4_6g33011 FvH4_6g34501 FvH4_6g50161 FvH4_7g04111 FvH4_7g04120 FvH4_7g04121 FvH4_7g04122 FvH4_7g04123 FvH4_7g28231 FvH4_c7g00010
malus_domestica MD12G1084200.v1.1
prunus_persica Prupe.1G206200_v2.0.a1 Prupe.8G085600_v2.0.a1
pyrus_communis pycom01g11080 pycom02g20500 pycom09g18370 pycom12555g00210 pycom13g23120 pycom14g10590 pycom16g17620 pycom16g22750
rosa_chinensis RchiOBHm_Chr2g0137481 RchiOBHm_Chr6g0251961 RchiOBHm_Chr7g0185821
rosa_laevigata RLG00000005585 RLG00000010281 RLG00000010912 RLG00000030342 RLG00000034281
rosa_multiflora Rmu_sc0000096.1_g000010 Rmu_sc0002481.1_g000045 Rmu_sc0003598.1_g000009 Rmu_sc0003863.1_g000019 Rmu_sc0003973.1_g000002 Rmu_sc0004823.1_g000050 Rmu_sc0005621.1_g000007 Rmu_sc0006537.1_g000030 Rmu_sc0006608.1_g000014 Rmu_sc0008506.1_g000007 Rmu_sc0014428.1_g000001 Rmu_sc0035437.1_g000001 Rmu_ssc0000396.1_g000022
rosa_roxburghii Rroxscaffold_175G00432300 Rroxscaffold_17G00435390 Rroxscaffold_27G00446590 Rroxscaffold_29G00441820 Rroxscaffold_31G00438110 Rroxscaffold_32G00442750 Rroxscaffold_33G00439810 Rroxscaffold_37G00445160 Rroxscaffold_4G00306500 Rroxscaffold_4G00327700 Rroxscaffold_4G00332630 Rroxscaffold_52G00439360 Rroxscaffold_63G00444420 Rroxscaffold_76G00448580 Rroxscaffold_7G00156270
rosa_rugosa Rorug03G0295200
rosa_samantha Rh1BG433700 Rh1CG115400 Rh2AG676300 Rh2CG341800 Rh2DG381400 Rh3BG370100 Rh3BG370200 Rh4AG441600 Rh4BG076500 Rh4BG107400 Rh5BG005600 Rh6BG010100 Rh7BG000200
rosa_wichuraiana Rw0G003820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 322
AatII GACGTC 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 322
AccII CGCG 1 cut(s) 9
AciI CCGC 2 cut(s) 152, 219
AclWI GGATC 2 cut(s) 68, 169
AcyI GRCGYC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 329
AlwI GGATC 2 cut(s) 68, 169
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 3 cut(s) 83, 106, 291
Asp700I GAANNNNTTC 1 cut(s) 29
AspA2I CCTAGG 1 cut(s) 56
AspLEI GCGC 1 cut(s) 9
AsuHPI GGTGA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 125
AvrII CCTAGG 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 305
BbvI GCAGC 3 cut(s) 93, 95, 303
BccI CCATC 1 cut(s) 85
BceAI ACGGC 1 cut(s) 101
BfaI CTAG 2 cut(s) 57, 126
BfuAI ACCTGC 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 3 cut(s) 84, 107, 292
BlnI CCTAGG 1 cut(s) 56
BlsI GCNGC 3 cut(s) 85, 108, 293
BmsI GCATC 4 cut(s) 70, 86, 108, 138
BmtI GCTAGC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 3 cut(s) 56, 140, 207
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 56, 140, 207
BseGI GGATG 4 cut(s) 96, 99, 101, 129
BseLI CCNNNNNNNGG 1 cut(s) 329
BseXI GCAGC 3 cut(s) 93, 95, 303
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 174
BsiSI CCGG 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 329
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 60, 161
BspACI CCGC 2 cut(s) 152, 219
BspANI GGCC 1 cut(s) 267
BspDI ATCGAT 1 cut(s) 174
BspFNI CGCG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 322
BspOI GCTAGC 1 cut(s) 129
BspPI GGATC 2 cut(s) 68, 169
BssECI CCNNGG 3 cut(s) 56, 140, 207
BssMI GATC 2 cut(s) 60, 161
BssNI GRCGYC 1 cut(s) 242
BssT1I CCWWGG 3 cut(s) 56, 140, 207
Bst4CI ACNGT 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 127
BstF5I GGATG 4 cut(s) 96, 99, 101, 129
BstFNI CGCG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 2 cut(s) 63, 164
BstMBI GATC 2 cut(s) 60, 161
BstMWI GCNNNNNNNGC 2 cut(s) 35, 122
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 3 cut(s) 93, 95, 303
BstV2I GAAGAC 1 cut(s) 305
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 267
BsuTUI ATCGAT 1 cut(s) 174
BtsCI GGATG 4 cut(s) 96, 99, 101, 129
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 127
CciI TCATGA 1 cut(s) 237
CfoI GCGC 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 174
CspCI CAANNNNNGTGG 1 cut(s) 307
CviAII CATG 1 cut(s) 238
CviJI RGCY 5 cut(s) 38, 55, 106, 125, 267
CviKI_1 RGCY 5 cut(s) 38, 55, 106, 125, 267
DpnI GATC 2 cut(s) 62, 163
DpnII GATC 2 cut(s) 60, 161
Eco130I CCWWGG 3 cut(s) 56, 140, 207
EcoT14I CCWWGG 3 cut(s) 56, 140, 207
EcoT22I ATGCAT 1 cut(s) 101
ErhI CCWWGG 3 cut(s) 56, 140, 207
FaeI CATG 1 cut(s) 241
FaiI YATR 4 cut(s) 35, 171, 231, 239
FatI CATG 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 84, 107, 292
FokI GGATG 4 cut(s) 86, 103, 108, 116
Fsp4HI GCNGC 3 cut(s) 84, 107, 292
FspBI CTAG 2 cut(s) 57, 126
GlaI GCGC 1 cut(s) 8
GluI GCNGC 3 cut(s) 84, 107, 292
HaeIII GGCC 1 cut(s) 267
HapII CCGG 1 cut(s) 103
HhaI GCGC 1 cut(s) 9
Hin1I GRCGYC 1 cut(s) 242
Hin1II CATG 1 cut(s) 241
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HinfI GANTC 3 cut(s) 199, 273, 284
HpaII CCGG 1 cut(s) 103
HphI GGTGA 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 247, 272
Hpy188III TCNNGA 2 cut(s) 238, 277
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 3 cut(s) 115, 149, 274
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 3 cut(s) 49, 83, 99
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 122
HpySE526I ACGT 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 242
Hsp92II CATG 1 cut(s) 241
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 2 cut(s) 60, 161
LpnPI CCDG 2 cut(s) 116, 327
Lsp1109I GCAGC 3 cut(s) 93, 95, 303
LweI GCATC 4 cut(s) 70, 86, 108, 138
MaeI CTAG 2 cut(s) 57, 126
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 62, 163
MboI GATC 2 cut(s) 60, 161
MboII GAAGA 3 cut(s) 86, 260, 310
MlyI GAGTC 2 cut(s) 282, 293
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 5 cut(s) 27, 58, 64, 144, 278
Mph1103I ATGCAT 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 29
MspI CCGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 29
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 35, 122
NdeII GATC 2 cut(s) 60, 161
NheI GCTAGC 1 cut(s) 125
NlaIII CATG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 274
NsiI ATGCAT 1 cut(s) 101
PagI TCATGA 1 cut(s) 237
PaqCI CACCTGC 1 cut(s) 322
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 3 cut(s) 85, 108, 293
PleI GAGTC 2 cut(s) 281, 292
PpsI GAGTC 2 cut(s) 281, 292
SatI GCNGC 3 cut(s) 84, 107, 292
Sau3AI GATC 2 cut(s) 60, 161
SchI GAGTC 2 cut(s) 282, 293
SetI ASST 5 cut(s) 47, 160, 245, 316, 325
SfaNI GCATC 4 cut(s) 70, 86, 108, 138
SsiI CCGC 2 cut(s) 152, 219
SspMI CTAG 2 cut(s) 57, 126
StyI CCWWGG 3 cut(s) 56, 140, 207
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 245
TaqI TCGA 2 cut(s) 63, 174
TfiI GAWTC 1 cut(s) 199
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 3 cut(s) 83, 106, 291
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 22, 226
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmaJI CCTAGG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 2 cut(s) 57, 126
ZraI GACGTC 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.