Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 61 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 88 |
| AluBI | AGCT | 2 cut(s) | 46, 67 |
| AluI | AGCT | 2 cut(s) | 46, 67 |
| BccI | CCATC | 3 cut(s) | 43, 82, 86 |
| BciVI | GTATCC | 1 cut(s) | 120 |
| BfmI | CTRYAG | 1 cut(s) | 112 |
| BfuI | GTATCC | 1 cut(s) | 120 |
| BmsI | GCATC | 1 cut(s) | 19 |
| BpuEI | CTTGAG | 1 cut(s) | 123 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 88 |
| BseGI | GGATG | 1 cut(s) | 78 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BsmI | GAATGC | 1 cut(s) | 32 |
| BstF5I | GGATG | 1 cut(s) | 78 |
| BstSFI | CTRYAG | 1 cut(s) | 112 |
| BsuI | GTATCC | 1 cut(s) | 120 |
| BtgZI | GCGATG | 1 cut(s) | 101 |
| BtsCI | GGATG | 1 cut(s) | 78 |
| Csp6I | GTAC | 1 cut(s) | 60 |
| CviJI | RGCY | 3 cut(s) | 20, 46, 67 |
| CviKI_1 | RGCY | 3 cut(s) | 20, 46, 67 |
| CviQI | GTAC | 1 cut(s) | 60 |
| EcoT22I | ATGCAT | 1 cut(s) | 34 |
| FokI | GGATG | 1 cut(s) | 65 |
| Hpy188I | TCNGA | 1 cut(s) | 17 |
| Hpy188III | TCNNGA | 1 cut(s) | 37 |
| HpyAV | CCTTC | 1 cut(s) | 112 |
| HpyCH4V | TGCA | 1 cut(s) | 32 |
| LpnPI | CCDG | 4 cut(s) | 22, 100, 114, 144 |
| LweI | GCATC | 1 cut(s) | 19 |
| MaeIII | GTNAC | 1 cut(s) | 103 |
| MboII | GAAGA | 1 cut(s) | 20 |
| MnlI | CCTC | 2 cut(s) | 143, 147 |
| Mph1103I | ATGCAT | 1 cut(s) | 34 |
| Mva1269I | GAATGC | 1 cut(s) | 32 |
| NmuCI | GTSAC | 1 cut(s) | 103 |
| NsiI | ATGCAT | 1 cut(s) | 34 |
| PctI | GAATGC | 1 cut(s) | 32 |
| RsaI | GTAC | 1 cut(s) | 61 |
| RsaNI | GTAC | 1 cut(s) | 60 |
| SetI | ASST | 6 cut(s) | 48, 69, 75, 103, 123, 139 |
| SfaNI | GCATC | 1 cut(s) | 19 |
| SfcI | CTRYAG | 1 cut(s) | 112 |
| SgeI | CNNG | 8 cut(s) | 29, 49, 55, 76, 95, 113, 127, 143 |
| SmlI | CTYRAG | 1 cut(s) | 138 |
| SmoI | CTYRAG | 1 cut(s) | 138 |
| TseFI | GTSAC | 1 cut(s) | 103 |
| Tsp45I | GTSAC | 1 cut(s) | 103 |
| TspGWI | ACGGA | 1 cut(s) | 122 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 85 |
| Zsp2I | ATGCAT | 1 cut(s) | 34 |