FvH4_6g29581

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000353
Physical Location & Seq
Forward (+)
22833109 .. 22834674
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29581.t1

Sequence Viewer

Length: 1149 bp
ATGGCATTGAAACTAGCGGAGAAAGAGAAAGAAAAATGTAGGGCAGCTATCAAAGAAGCAGAAGCAGCTCAAAGGGTTGCCAAATTAGAAGCACAAAAGGCGAGGATTGCAGAAATGCAAGATCTGAATCAAGCAGATGAGGGGAGGGAGGTATCAGAATCAATGACGTATGATCTAAGGTACAGGAAATACACAATTGAGGAGCTTGAAGCAGCAACAAATGATTTTTCACAAGCTAACAAAATTGGGGAAGGAAGCTATGGGCCAGTATACGTAGGTGAAATAGACCATACACGAGTGGCAATCAAAGTACTCCGTCCTGATGCAGCTCAAGGAAGGTTGCAGTTCCAAAGAGAGGTTGAAATATTGAGCTGCATACGGCATCCAAATATGGTTCTCCTTCTTGGAGCCTGCCCTGAGTATGGTTGCCTGGTCTACGAGTACATGGCTAATGGTAGCTTAGAGGACTGTCTCTTCCAGCGATGTAACACACAAGTTATTCCTTGGCAGCTAAGGTTCAAAATTGCAGCTGAAATCTGCACTGGGCTTTACTTTCTTCATCAAAATAAGCCAGAACCCCTAGTACACCGTGATCTCAAACCTGGAAACATTTTGCTAGACCATAACTATGTTAGCAAGATTAGTGATGTAGGTTTGGGCAGGCTTGTCCCTCAATCAGTAGCTGATTCGGTCACTCAGTATCGCATGACATCTACAGCAGGAACATTCTGTTATATCGATCCTGAGTACCAGCAAACAGGCATGCTTGCAACAAAGTCTGATGTCTACTCACTTGGTGTCATGCTTTTACAACTAATAACAGCCAAGCCACCGATGGGCCTGACTCATCATGTTATGGAAGCTATTGAGATAGGGAATTTTGCTCAGATTCTTGACCCTGCTGTTCCTGACTGGCCTGTTGAAGAAGCCTTGAAGTTTGCCAAACTAGGTTTACAGTGTGCAGAAATGAGGAGAAAAGACAGACCGGATCTTGGCAAGGTTGTGCTACCTGAGCTCAATAGATTGAGTGCACTTGCCGAAGAAAGCATGCATGGTGTCATGCATAGTAGTAGTAGAGCTGGAAGAGTAATCTTACCAAAACAAGTACTACGCGCCAGTTTGACAATACAAGTGAGTAGCAGAACCTAA

Protein Analysis

383

Amino Acids

42.77

Weight (kDa)

6.34

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 77 - 329 3.1e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 78 - 334 2.9e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 270, 435, 786
AccII CGCG 1 cut(s) 1113
AciI CCGC 1 cut(s) 17
AclWI GGATC 2 cut(s) 734, 996
AcsI RAATTY 1 cut(s) 877
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 6 cut(s) 182, 312, 443, 585, 749, 1107
AfiI CCNNNNNNNGG 4 cut(s) 355, 422, 836, 992
AgsI TTSAA 6 cut(s) 10, 209, 362, 520, 923, 934
AjnI CCWGG 2 cut(s) 429, 601
Alw21I GWGCWC 2 cut(s) 1017, 1033
Alw26I GTCTC 1 cut(s) 476
Alw44I GTGCAC 1 cut(s) 1029
AlwI GGATC 2 cut(s) 734, 996
AlwNI CAGNNNCTG 1 cut(s) 683
AoxI GGCC 3 cut(s) 263, 838, 914
ApaLI GTGCAC 1 cut(s) 1029
ApeKI GCWGC 7 cut(s) 44, 65, 212, 326, 372, 508, 527
ApoI RAATTY 1 cut(s) 877
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 2 cut(s) 263, 838
AsuHPI GGTGA 1 cut(s) 290
BaeGI GKGCMC 1 cut(s) 1033
BanII GRGCYC 1 cut(s) 1017
BauI CACGAG 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 1017, 1033
BbvI GCAGC 7 cut(s) 56, 77, 224, 338, 359, 520, 539
BccI CCATC 1 cut(s) 829
BceAI ACGGC 1 cut(s) 395
BciT130I CCWGG 2 cut(s) 431, 603
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 4 cut(s) 14, 581, 617, 947
BfmI CTRYAG 1 cut(s) 714
BglII AGATCT 1 cut(s) 121
BisI GCNGC 7 cut(s) 45, 66, 213, 327, 373, 509, 528
BlsI GCNGC 7 cut(s) 46, 67, 214, 328, 374, 510, 529
BmcAI AGTACT 2 cut(s) 312, 1107
Bme1390I CCNGG 2 cut(s) 431, 603
BmgT120I GGNCC 2 cut(s) 263, 838
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 2 cut(s) 431, 603
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 2 cut(s) 313, 391
BmuI ACTGGG 1 cut(s) 552
Bpu10I CCTNAGC 2 cut(s) 512, 1011
BpuEI CTTGAG 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 503
BsaWI WCCGGW 1 cut(s) 985
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 422, 836, 992
Bse1I ACTGG 4 cut(s) 266, 547, 917, 1116
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 2 cut(s) 431, 603
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 1 cut(s) 382
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 4 cut(s) 355, 422, 836, 992
BseMII CTCAG 5 cut(s) 408, 710, 735, 899, 1002
BseNI ACTGG 4 cut(s) 266, 547, 917, 1116
BseRI GAGGAG 2 cut(s) 215, 985
BseSI GKGCMC 1 cut(s) 1033
BseXI GCAGC 7 cut(s) 56, 77, 224, 338, 359, 520, 539
BsgI GTGCAG 2 cut(s) 523, 981
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 3 cut(s) 265, 840, 916
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 2 cut(s) 1017, 1033
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 4 cut(s) 355, 422, 836, 992
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 3 cut(s) 265, 840, 916
Bsp1286I GDGCHC 2 cut(s) 1017, 1033
Bsp143I GATC 5 cut(s) 121, 172, 592, 739, 988
BspACI CCGC 1 cut(s) 17
BspANI GGCC 3 cut(s) 265, 840, 916
BspCNI CTCAG 5 cut(s) 409, 709, 736, 898, 1003
BspDI ATCGAT 1 cut(s) 738
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 2 cut(s) 734, 996
BsrI ACTGG 4 cut(s) 266, 547, 917, 1116
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 5 cut(s) 121, 172, 592, 739, 988
BssNAI GTATAC 1 cut(s) 271
BssSI CACGAG 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 503
Bst1107I GTATAC 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 431, 603
Bst4CI ACNGT 3 cut(s) 470, 590, 957
Bst6I CTCTTC 2 cut(s) 479, 1078
BstBAI YACGTR 1 cut(s) 274
BstC8I GCNNGC 5 cut(s) 412, 662, 764, 768, 1049
BstDEI CTNAG 8 cut(s) 176, 417, 460, 512, 696, 744, 885, 1011
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 5 cut(s) 124, 175, 595, 742, 991
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 5 cut(s) 121, 172, 592, 739, 988
BstMWI GCNNNNNNNGC 4 cut(s) 65, 98, 107, 1012
BstNI CCWGG 2 cut(s) 431, 603
BstNSI RCATGY 2 cut(s) 766, 1051
BstSCI CCNGG 2 cut(s) 429, 601
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 1033
BstSNI TACGTA 1 cut(s) 274
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 7 cut(s) 56, 77, 224, 338, 359, 520, 539
BstX2I RGATCY 2 cut(s) 121, 988
BstYI RGATCY 2 cut(s) 121, 988
BstZ17I GTATAC 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 738
BsuRI GGCC 3 cut(s) 265, 840, 916
BsuTUI ATCGAT 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 496
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 540, 962
Cac8I GCNNGC 5 cut(s) 412, 662, 764, 768, 1049
CaiI CAGNNNCTG 1 cut(s) 683
CfoI GCGC 1 cut(s) 1115
Cfr13I GGNCC 2 cut(s) 263, 838
ClaI ATCGAT 1 cut(s) 738
Csp6I GTAC 6 cut(s) 181, 311, 442, 584, 748, 1106
CviAII CATG 8 cut(s) 445, 706, 763, 802, 851, 1048, 1052, 1060
CviQI GTAC 6 cut(s) 181, 311, 442, 584, 748, 1106
DdeI CTNAG 8 cut(s) 176, 417, 460, 512, 696, 744, 885, 1011
DpnI GATC 5 cut(s) 123, 174, 594, 741, 990
DpnII GATC 5 cut(s) 121, 172, 592, 739, 988
DraIII CACNNNGTG 1 cut(s) 797
Eam1104I CTCTTC 2 cut(s) 479, 1078
EarI CTCTTC 2 cut(s) 479, 1078
Ecl136II GAGCTC 1 cut(s) 1015
Eco105I TACGTA 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 1017
Eco53kI GAGCTC 1 cut(s) 1015
EcoICRI GAGCTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 429, 601
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 2 cut(s) 1053, 1065
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 8 cut(s) 448, 709, 766, 805, 854, 1051, 1055, 1063
FaqI GGGAC 1 cut(s) 653
FatI CATG 8 cut(s) 444, 705, 762, 801, 850, 1047, 1051, 1059
FblI GTMKAC 3 cut(s) 270, 435, 786
Fnu4HI GCNGC 7 cut(s) 45, 66, 213, 327, 373, 509, 528
FokI GGATG 1 cut(s) 369
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 7 cut(s) 45, 66, 213, 327, 373, 509, 528
FspBI CTAG 4 cut(s) 14, 581, 617, 947
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 7 cut(s) 45, 66, 213, 327, 373, 509, 528
HaeIII GGCC 3 cut(s) 265, 840, 916
HapII CCGG 1 cut(s) 986
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 8 cut(s) 448, 709, 766, 805, 854, 1051, 1055, 1063
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HinfI GANTC 5 cut(s) 127, 158, 686, 844, 889
HpaII CCGG 1 cut(s) 986
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 6 cut(s) 271, 436, 586, 787, 953, 1031
Hpy188I TCNGA 4 cut(s) 126, 157, 781, 888
Hpy188III TCNNGA 4 cut(s) 320, 743, 893, 908
Hpy8I GTNNAC 6 cut(s) 271, 436, 586, 787, 953, 1031
HpyAV CCTTC 3 cut(s) 245, 330, 410
HpyCH4III ACNGT 3 cut(s) 470, 590, 957
HpyCH4IV ACGT 2 cut(s) 167, 273
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 98, 107, 1012
HpyF3I CTNAG 8 cut(s) 176, 417, 460, 512, 696, 744, 885, 1011
HpySE526I ACGT 2 cut(s) 167, 273
Hsp92II CATG 8 cut(s) 448, 709, 766, 805, 854, 1051, 1055, 1063
HspAI GCGC 1 cut(s) 1113
Kzo9I GATC 5 cut(s) 121, 172, 592, 739, 988
LmnI GCTCC 2 cut(s) 202, 407
Lsp1109I GCAGC 7 cut(s) 56, 77, 224, 338, 359, 520, 539
LweI GCATC 2 cut(s) 313, 391
MaeI CTAG 4 cut(s) 14, 581, 617, 947
MaeII ACGT 2 cut(s) 167, 273
MaeIII GTNAC 2 cut(s) 485, 691
MalI GATC 5 cut(s) 123, 174, 594, 741, 990
MboI GATC 5 cut(s) 121, 172, 592, 739, 988
MboII GAAGA 5 cut(s) 466, 548, 935, 1052, 1095
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 2 cut(s) 121, 988
MhlI GDGCHC 2 cut(s) 1017, 1033
MluCI AATT 5 cut(s) 83, 195, 243, 522, 877
MlyI GAGTC 1 cut(s) 838
MnlI CCTC 9 cut(s) 96, 133, 138, 142, 193, 349, 457, 681, 963
Mph1103I ATGCAT 2 cut(s) 1053, 1065
MslI CAYNNNNRTG 1 cut(s) 627
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 2 cut(s) 431, 603
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 2 cut(s) 431, 603
MvnI CGCG 1 cut(s) 1113
MwoI GCNNNNNNNGC 4 cut(s) 65, 98, 107, 1012
NdeII GATC 5 cut(s) 121, 172, 592, 739, 988
NlaIII CATG 8 cut(s) 448, 709, 766, 805, 854, 1051, 1055, 1063
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 691
NsiI ATGCAT 2 cut(s) 1053, 1065
NspI RCATGY 2 cut(s) 766, 1051
PaeI GCATGC 2 cut(s) 766, 1051
PfeI GAWTC 4 cut(s) 127, 158, 686, 889
PkrI GCNGC 7 cut(s) 46, 67, 214, 328, 374, 510, 529
PleI GAGTC 1 cut(s) 838
PpsI GAGTC 1 cut(s) 838
Ppu21I YACGTR 1 cut(s) 274
Psp124BI GAGCTC 1 cut(s) 1017
Psp6I CCWGG 2 cut(s) 429, 601
PspGI CCWGG 2 cut(s) 429, 601
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 2 cut(s) 263, 838
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 2 cut(s) 121, 988
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 6 cut(s) 182, 312, 443, 585, 749, 1107
RsaNI GTAC 6 cut(s) 181, 311, 442, 584, 748, 1106
RseI CAYNNNNRTG 1 cut(s) 627
SacI GAGCTC 1 cut(s) 1017
SatI GCNGC 7 cut(s) 45, 66, 213, 327, 373, 509, 528
Sau3AI GATC 5 cut(s) 121, 172, 592, 739, 988
Sau96I GGNCC 2 cut(s) 263, 838
ScaI AGTACT 2 cut(s) 312, 1107
SchI GAGTC 1 cut(s) 838
ScrFI CCNGG 2 cut(s) 431, 603
SduI GDGCHC 2 cut(s) 1017, 1033
SfaNI GCATC 2 cut(s) 313, 391
SfcI CTRYAG 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
SnaBI TACGTA 1 cut(s) 274
SphI GCATGC 2 cut(s) 766, 1051
Sse9I AATT 5 cut(s) 83, 195, 243, 522, 877
SsiI CCGC 1 cut(s) 17
SspI AATATT 1 cut(s) 366
SspMI CTAG 4 cut(s) 14, 581, 617, 947
SstI GAGCTC 1 cut(s) 1017
StyD4I CCNGG 2 cut(s) 429, 601
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 3 cut(s) 470, 590, 957
TaiI ACGT 2 cut(s) 170, 276
TaqI TCGA 1 cut(s) 738
TaqII GACCGA 1 cut(s) 679
TasI AATT 5 cut(s) 83, 195, 243, 522, 877
TatI WGTACW 4 cut(s) 310, 441, 583, 1105
TfiI GAWTC 4 cut(s) 127, 158, 686, 889
TscAI CASTG 2 cut(s) 547, 962
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 7 cut(s) 44, 65, 212, 326, 372, 508, 527
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 1 cut(s) 548
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 547, 962
VneI GTGCAC 1 cut(s) 1029
XapI RAATTY 1 cut(s) 877
XceI RCATGY 2 cut(s) 766, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XmiI GTMKAC 3 cut(s) 270, 435, 786
XspI CTAG 4 cut(s) 14, 581, 617, 947
ZrmI AGTACT 2 cut(s) 312, 1107
Zsp2I ATGCAT 2 cut(s) 1053, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.