Rw1G009960

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000353
Physical Location & Seq
Forward (+)
22100050 .. 22104699
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009960.1

Sequence Viewer

Length: 2310 bp
ATGAATACACACACTTCTAGAGAATATAACATTCTGATAGAGAGAATAAGCAAACTGAGAAAGACAAATTTAGGGCTTAGGTGTGTTCGAGAGAGAGAGAGACAGAGACAGACAGTGATGGCAAGATCATGTGCCACAAGTGATGAAAAGAGAGAACAGGTGGTGGCGGTGGCAATAGACAAGGACAAGGGAAGTATAAAATTGTGCAAAAGCCAATCTGATGCTGAATATAGCGAGTTGTTTCTTCCACTTCGTACTTTTTGTAAGAAAAAGCTTGTAAGATACAACGAAGTAATAGTTAAAGGTACCGATGTAGCAAAAGCACTAGTCAATTTTATTTCGACACATTCAATTGAGGTTTTGGTACTTGCGGCACCTTCAAAAAGTGGCTTTTTCAGAGCCTTCAACAAAACTACAGACATTCCAAGCAGAGTGTCGAAAGGGGCACCGGATTTCTGCACTGTGTATGTCATTGGCAAGGGAAAGATCAGTCATGTGCAAACAGCAACCACTAAATTACCCCCAGAAGCTCGTAATAAGCAAATACATAACCAACTCAGCAATGTTTCGGACTCAAGTAGGACAAGAACAAAGTACCGGCACCGTAGCGCTCGGATAGATCATGATACAGGAATCAAGACACCATTCACCAGAGGACAAGCTTCATCAAACAGATTATATGAGATCTCACCAGACAGCGATGTATCATTCACAAGTAGTGGAGGAAGACCAAGCATTGATCACATGTTTCCTTCTATATGCGGCAGTGTGGAATCAGGAACGCACTCTCAACAATCATTCAACTCAGACTCAGACAACAGAAGATCTACCTCGAAGCAATCCTACTCAAGAACCCAGTTCATTGACATGAACTCCTCATTATCCTCAACCGAGAGTGGAAAATCATGGTCATCCCGAAATATGGAAGAAGTAGAAGATGAGATGATGAGACTCAAGCTAGAGCTCAAGCAGACAATGGAGGTGTACAATACGGCCTGTAAGGAAACCCTAACATCAAAACAGAAGCAACTTGACCAATGGAAATGGGAAGAAGAACAAAGATTGGAAGGGGCACACCTAGGTCAGGAGTTTGCATTGGCACTTGTACAGAAAGAGGAAGCAAAATGTAGGGCAGCAATGGACGCAGAAAGAGCAGCTAAAAGGATTGCTGAATTGGAAGCAGAACAAAGGAGGAATTCAGAAATAAAGGGTCTGAAAGAAGCTGAAAAGAGGATGAACTCACTAGGAGGAAGAAGTTTTGATCTGCGGTACCGGAAATACACAATTGAAGAGATTAAAGCAGCAACGAACAGTTTTTCAAAAGCTCTTAAGATTGGAGAAGGTGGCTATGGGCCTGTGTATGTGGGTGAGCTGGACCATACACAAGTGGCAATAAAAGCTCTACGTCCAGATGCAGCTCAAGGACGTTCACAATTCCAACAAGAGATTGAAGTTTTGAGCTGCATAAGACATCCAAACATGGTTCTCCTGCTTGGAGCCTGCCCAGAGTATGGTTGTCTGATCTATGAGTACATGGGTAATGGGAGCTTAGAAGACCGTCTGTTTCGACGAGGTAACACCCCAGTTATTCCTTGGCAGCTAAGGTTCCGAATCGCTGCAGAAATTGCCACGGGGCTTTTGTTCCTTCACCAGGCCAAGCCAGAACCCTTTGTACACCGTGACTTAAAGCCAGGAAACATTTTGCTAGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTCTGGCCAGGCTTGTCCCTCCATCAGTAGCTGACTCAGTCACTCAGTATCACATGACATCAGCAGCTGGGACATTCTGTTATATCGACCCAGAGTATCAACAAACTGGCTTGCTTGGAACAAAATCTGATATCTACTCACTTGGGGTAATGCTTCTACAAATAATAACAGCCAAACCAGCAATGGGTTTGACTCATCTTGTTGAGCACGCAATTGAGATAGGGACCTTCGCTGAGATGCTTGACCCCGCTGTTCCTGACTGGCCTGTGGAGGAAGCCTTGAAGTTTGCCAAGTTAGCATTACAATGTGCCGAAATGAGGCGAAAAGACAGACCCGATCTTGGCAAGGTAGTGCTACCTGAGCTTAACAGATTCAGAGAACTCGGCGAAAAGGGTGGCATGCTTAATGGTGGTGGAGCATTCTTACTACAAAAGTGCTCAGTTTCCACAAAGCAAGAAGTGATGAATGACCCCCAATCTGGATATGCCAGTTCAGTGAGCAATTCGAGCACATCATCTTATGGAGGAAAGAATATTATAGTCGGATTCTTGACGTTTTCTTCGGTATAA

Protein Analysis

769

Amino Acids

85.93

Weight (kDa)

9.01

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 441 - 692 2.9e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 444 - 697 4.2e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 305, 1269
AccB1I GGYRCC 5 cut(s) 305, 373, 445, 600, 1269
AccB7I CCANNNNNTGG 1 cut(s) 1511
AciI CCGC 5 cut(s) 167, 371, 762, 1267, 1989
AcoI YGGCCR 1 cut(s) 1746
AcsI RAATTY 2 cut(s) 67, 1195
AfaI GTAC 9 cut(s) 256, 307, 366, 596, 986, 1107, 1271, 1532, 1674
AfeI AGCGCT 1 cut(s) 610
AfiI CCNNNNNNNGG 8 cut(s) 922, 1084, 1511, 1651, 1925, 2058, 2081, 2219
AflII CTTAAG 1 cut(s) 1328
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 8 cut(s) 351, 381, 406, 802, 1289, 1320, 1451, 2023
AhlI ACTAGT 1 cut(s) 325
AjnI CCWGG 3 cut(s) 1650, 1690, 1748
Alw21I GWGCWC 4 cut(s) 966, 1950, 2180, 2252
Alw26I GTCTC 3 cut(s) 94, 100, 943
AlwNI CAGNNNCTG 2 cut(s) 1772, 1808
Aor51HI AGCGCT 1 cut(s) 610
AoxI GGCC 5 cut(s) 993, 1352, 1653, 1746, 2003
ApeKI GCWGC 8 cut(s) 1133, 1154, 1301, 1415, 1461, 1597, 1616, 1805
ApoI RAATTY 2 cut(s) 67, 1195
ArsI GACNNNNNNTTYG 1 cut(s) 2284
Asp718I GGTACC 2 cut(s) 305, 1269
AspA2I CCTAGG 1 cut(s) 1078
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 3 cut(s) 1352, 1375, 1965
AsuHPI GGTGA 4 cut(s) 640, 681, 1379, 1640
AvaII GGWCC 2 cut(s) 1375, 1965
AvrII CCTAGG 1 cut(s) 1078
BaeGI GKGCMC 2 cut(s) 448, 1075
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BalI TGGCCA 1 cut(s) 1748
BanI GGYRCC 5 cut(s) 305, 373, 445, 600, 1269
BanII GRGCYC 1 cut(s) 966
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BbsI GAAGAC 2 cut(s) 733, 1560
Bbv12I GWGCWC 4 cut(s) 966, 1950, 2180, 2252
BbvI GCAGC 8 cut(s) 1145, 1166, 1313, 1427, 1448, 1603, 1609, 1817
BccI CCATC 2 cut(s) 112, 1771
BceAI ACGGC 1 cut(s) 1008
BciT130I CCWGG 3 cut(s) 1652, 1692, 1750
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 94, 100, 943
BcuI ACTAGT 1 cut(s) 325
BfaI CTAG 6 cut(s) 18, 326, 959, 1079, 1244, 1706
BfmI CTRYAG 2 cut(s) 414, 1617
BfoI RGCGCY 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 1328
BglII AGATCT 2 cut(s) 684, 824
BlnI CCTAGG 1 cut(s) 1078
Bme1390I CCNGG 3 cut(s) 1652, 1692, 1750
Bme18I GGWCC 2 cut(s) 1375, 1965
BmgT120I GGNCC 3 cut(s) 1352, 1375, 1965
BmiI GGNNCC 8 cut(s) 307, 375, 447, 602, 1271, 1498, 1607, 1966
BmrFI CCNGG 3 cut(s) 1652, 1692, 1750
BmrI ACTGGG 2 cut(s) 850, 1577
BmsI GCATC 3 cut(s) 211, 1402, 1968
BmuI ACTGGG 2 cut(s) 850, 1577
BpiI GAAGAC 2 cut(s) 733, 1560
Bpu10I CCTNAGC 3 cut(s) 77, 1601, 2100
BpuEI CTTGAG 5 cut(s) 559, 832, 938, 950, 1404
BsaJI CCNNGG 3 cut(s) 1078, 1592, 1629
BsaWI WCCGGW 2 cut(s) 448, 1272
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bsc4I CCNNNNNNNGG 8 cut(s) 922, 1084, 1511, 1651, 1925, 2058, 2081, 2219
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 5 cut(s) 856, 1583, 1852, 2006, 2229
Bse3DI GCAATG 3 cut(s) 568, 1143, 1929
BseBI CCWGG 3 cut(s) 1652, 1692, 1750
BseDI CCNNGG 3 cut(s) 1078, 1592, 1629
BseGI GGATG 3 cut(s) 911, 1239, 1471
BseLI CCNNNNNNNGG 8 cut(s) 922, 1084, 1511, 1651, 1925, 2058, 2081, 2219
BseMI GCAATG 3 cut(s) 568, 1143, 1929
BseMII CTCAG 9 cut(s) 47, 571, 819, 825, 1791, 1799, 1965, 2091, 2193
BseNI ACTGG 5 cut(s) 856, 1583, 1852, 2006, 2229
BseRI GAGGAG 1 cut(s) 865
BseSI GKGCMC 2 cut(s) 448, 1075
BseXI GCAGC 8 cut(s) 1145, 1166, 1313, 1427, 1448, 1603, 1609, 1817
BseYI CCCAGC 1 cut(s) 1808
BsgI GTGCAG 1 cut(s) 442
BshFI GGCC 5 cut(s) 995, 1354, 1655, 1748, 2005
BshNI GGYRCC 5 cut(s) 305, 373, 445, 600, 1269
BsiHKAI GWGCWC 4 cut(s) 966, 1950, 2180, 2252
BsiSI CCGG 3 cut(s) 449, 598, 1273
BslFI GGGAC 3 cut(s) 1742, 1825, 1978
BslI CCNNNNNNNGG 8 cut(s) 922, 1084, 1511, 1651, 1925, 2058, 2081, 2219
BsmAI GTCTC 3 cut(s) 94, 100, 943
BsmFI GGGAC 3 cut(s) 1742, 1825, 1978
BsmI GAATGC 1 cut(s) 2159
BsnI GGCC 5 cut(s) 995, 1354, 1655, 1748, 2005
Bsp1286I GDGCHC 6 cut(s) 448, 966, 1075, 1950, 2180, 2252
Bsp1407I TGTACA 3 cut(s) 984, 1105, 1672
Bsp143I GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
BspACI CCGC 5 cut(s) 167, 371, 762, 1267, 1989
BspANI GGCC 5 cut(s) 995, 1354, 1655, 1748, 2005
BspCNI CTCAG 9 cut(s) 48, 570, 818, 824, 1790, 1798, 1966, 2092, 2192
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 8 cut(s) 307, 375, 447, 602, 1271, 1498, 1607, 1966
BspMAI CTGCAG 1 cut(s) 1621
BspT107I GGYRCC 5 cut(s) 305, 373, 445, 600, 1269
BspTI CTTAAG 1 cut(s) 1328
BsrDI GCAATG 3 cut(s) 568, 1143, 1929
BsrFI RCCGGY 1 cut(s) 597
BsrGI TGTACA 3 cut(s) 984, 1105, 1672
BsrI ACTGG 5 cut(s) 856, 1583, 1852, 2006, 2229
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 3 cut(s) 1078, 1592, 1629
BssMI GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
BssT1I CCWWGG 2 cut(s) 1078, 1592
Bst2UI CCWGG 3 cut(s) 1652, 1692, 1750
Bst4CI ACNGT 6 cut(s) 115, 463, 605, 1313, 1559, 1679
Bst6I CTCTTC 1 cut(s) 1284
BstAFI CTTAAG 1 cut(s) 1328
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstAUI TGTACA 3 cut(s) 984, 1105, 1672
BstC8I GCNNGC 4 cut(s) 1501, 1853, 1950, 2141
BstDSI CCRYGG 1 cut(s) 1629
BstENI CCTNNNNNAGG 2 cut(s) 1082, 1649
BstF5I GGATG 3 cut(s) 911, 1239, 1471
BstH2I RGCGCY 1 cut(s) 612
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 9 cut(s) 128, 489, 622, 687, 742, 827, 1264, 1524, 2080
BstMAI GTCTC 3 cut(s) 94, 100, 943
BstMBI GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
BstMWI GCNNNNNNNGC 8 cut(s) 1142, 1151, 1397, 1625, 1919, 2036, 2101, 2247
BstNI CCWGG 3 cut(s) 1652, 1692, 1750
BstNSI RCATGY 2 cut(s) 748, 2143
BstSCI CCNGG 3 cut(s) 1650, 1690, 1748
BstSFI CTRYAG 2 cut(s) 414, 1617
BstSLI GKGCMC 2 cut(s) 448, 1075
BstV1I GCAGC 8 cut(s) 1145, 1166, 1313, 1427, 1448, 1603, 1609, 1817
BstV2I GAAGAC 2 cut(s) 733, 1560
BstX2I RGATCY 2 cut(s) 684, 824
BstYI RGATCY 2 cut(s) 684, 824
BsuRI GGCC 5 cut(s) 995, 1354, 1655, 1748, 2005
BtgI CCRYGG 1 cut(s) 1629
BtgZI GCGATG 1 cut(s) 714
BtsCI GGATG 3 cut(s) 911, 1239, 1471
BtsI GCAGTG 1 cut(s) 772
BtsIMutI CAGTG 4 cut(s) 120, 459, 772, 2241
Cac8I GCNNGC 4 cut(s) 1501, 1853, 1950, 2141
CaiI CAGNNNCTG 2 cut(s) 1772, 1808
CciI TCATGA 1 cut(s) 622
CfoI GCGC 1 cut(s) 611
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 3 cut(s) 1352, 1375, 1965
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 9 cut(s) 255, 306, 365, 595, 985, 1106, 1270, 1531, 1673
CviQI GTAC 9 cut(s) 255, 306, 365, 595, 985, 1106, 1270, 1531, 1673
DpnI GATC 9 cut(s) 127, 488, 621, 686, 741, 826, 1263, 1523, 2079
DpnII GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
EaeI YGGCCR 1 cut(s) 1746
Eam1104I CTCTTC 1 cut(s) 1284
EarI CTCTTC 1 cut(s) 1284
Ecl136II GAGCTC 1 cut(s) 964
Eco130I CCWWGG 2 cut(s) 1078, 1592
Eco24I GRGCYC 1 cut(s) 966
Eco32I GATATC 1 cut(s) 1873
Eco47I GGWCC 2 cut(s) 1375, 1965
Eco47III AGCGCT 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 964
EcoICRI GAGCTC 1 cut(s) 964
EcoNI CCTNNNNNAGG 2 cut(s) 1082, 1649
EcoO109I RGGNCCY 1 cut(s) 1965
EcoRI GAATTC 1 cut(s) 1195
EcoRII CCWGG 3 cut(s) 1650, 1690, 1748
EcoRV GATATC 1 cut(s) 1873
EcoT14I CCWWGG 2 cut(s) 1078, 1592
EcoT38I GRGCYC 1 cut(s) 966
ErhI CCWWGG 2 cut(s) 1078, 1592
FaqI GGGAC 3 cut(s) 1742, 1825, 1978
FauI CCCGC 1 cut(s) 1996
FbaI TGATCA 1 cut(s) 739
FokI GGATG 3 cut(s) 898, 1246, 1458
FriOI GRGCYC 1 cut(s) 966
FspBI CTAG 6 cut(s) 18, 326, 959, 1079, 1244, 1706
GlaI GCGC 1 cut(s) 610
GsaI CCCAGC 1 cut(s) 1812
HaeII RGCGCY 1 cut(s) 612
HaeIII GGCC 5 cut(s) 995, 1354, 1655, 1748, 2005
HapII CCGG 3 cut(s) 449, 598, 1273
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 1 cut(s) 611
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HindIII AAGCTT 2 cut(s) 272, 660
HpaII CCGG 3 cut(s) 449, 598, 1273
HphI GGTGA 4 cut(s) 640, 681, 1379, 1640
Hpy166II GTNNAC 3 cut(s) 985, 1430, 1675
Hpy8I GTNNAC 3 cut(s) 985, 1430, 1675
Hpy99I CGWCG 1 cut(s) 1572
HpyAV CCTTC 7 cut(s) 387, 412, 762, 1061, 1334, 1655, 1978
HpyCH4III ACNGT 6 cut(s) 115, 463, 605, 1313, 1559, 1679
HpyCH4IV ACGT 3 cut(s) 1405, 1426, 2294
HpyCH4V TGCA 7 cut(s) 207, 459, 499, 1094, 1415, 1464, 1619
HpyF10VI GCNNNNNNNGC 8 cut(s) 1142, 1151, 1397, 1625, 1919, 2036, 2101, 2247
HpySE526I ACGT 3 cut(s) 1405, 1426, 2294
HspAI GCGC 1 cut(s) 609
KpnI GGTACC 2 cut(s) 309, 1273
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
LmnI GCTCC 3 cut(s) 1496, 1545, 2156
Lsp1109I GCAGC 8 cut(s) 1145, 1166, 1313, 1427, 1448, 1603, 1609, 1817
LweI GCATC 3 cut(s) 211, 1402, 1968
MaeI CTAG 6 cut(s) 18, 326, 959, 1079, 1244, 1706
MaeII ACGT 3 cut(s) 1405, 1426, 2294
MaeIII GTNAC 3 cut(s) 1574, 1679, 1780
MalI GATC 9 cut(s) 127, 488, 621, 686, 741, 826, 1263, 1523, 2079
MboI GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
MfeI CAATTG 3 cut(s) 351, 1284, 1953
MflI RGATCY 2 cut(s) 684, 824
MhlI GDGCHC 6 cut(s) 448, 966, 1075, 1950, 2180, 2252
MlsI TGGCCA 1 cut(s) 1748
MluNI TGGCCA 1 cut(s) 1748
MlyI GAGTC 5 cut(s) 566, 803, 945, 1769, 1927
MmeI TCCRAC 2 cut(s) 1462, 2263
Mox20I TGGCCA 1 cut(s) 1748
MscI TGGCCA 1 cut(s) 1748
MseI TTAA 6 cut(s) 300, 1296, 1329, 1685, 2106, 2145
MslI CAYNNNNRTG 3 cut(s) 866, 1716, 2044
Msp20I TGGCCA 1 cut(s) 1748
MspA1I CMGCKG 2 cut(s) 1808, 1991
MspCI CTTAAG 1 cut(s) 1328
MspI CCGG 3 cut(s) 449, 598, 1273
MspR9I CCNGG 3 cut(s) 1652, 1692, 1750
MunI CAATTG 3 cut(s) 351, 1284, 1953
Mva1269I GAATGC 1 cut(s) 2159
MvaI CCWGG 3 cut(s) 1652, 1692, 1750
MwoI GCNNNNNNNGC 8 cut(s) 1142, 1151, 1397, 1625, 1919, 2036, 2101, 2247
NdeII GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
NlaIV GGNNCC 8 cut(s) 307, 375, 447, 602, 1271, 1498, 1607, 1966
NmeAIII GCCGAG 1 cut(s) 2103
NmuCI GTSAC 2 cut(s) 1679, 1780
NspI RCATGY 2 cut(s) 748, 2143
PaeI GCATGC 1 cut(s) 2143
PagI TCATGA 1 cut(s) 622
PciI ACATGT 1 cut(s) 744
PctI GAATGC 1 cut(s) 2159
PfeI GAWTC 5 cut(s) 633, 773, 1611, 2112, 2286
PflFI GACNNNGTC 1 cut(s) 1778
PflMI CCANNNNNTGG 1 cut(s) 1511
PleI GAGTC 5 cut(s) 566, 803, 945, 1769, 1927
PpsI GAGTC 5 cut(s) 566, 803, 945, 1769, 1927
PpuMI RGGWCCY 1 cut(s) 1965
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 966
Psp5II RGGWCCY 1 cut(s) 1965
Psp6I CCWGG 3 cut(s) 1650, 1690, 1748
PspFI CCCAGC 1 cut(s) 1808
PspGI CCWGG 3 cut(s) 1650, 1690, 1748
PspN4I GGNNCC 8 cut(s) 307, 375, 447, 602, 1271, 1498, 1607, 1966
PspPI GGNCC 3 cut(s) 1352, 1375, 1965
PspPPI RGGWCCY 1 cut(s) 1965
PsrI GAACNNNNNNTAC 2 cut(s) 1656, 1688
PstI CTGCAG 1 cut(s) 1621
PstNI CAGNNNCTG 2 cut(s) 1772, 1808
PsuI RGATCY 2 cut(s) 684, 824
PsyI GACNNNGTC 1 cut(s) 1778
PvuII CAGCTG 1 cut(s) 1808
RsaI GTAC 9 cut(s) 256, 307, 366, 596, 986, 1107, 1271, 1532, 1674
RsaNI GTAC 9 cut(s) 255, 306, 365, 595, 985, 1106, 1270, 1531, 1673
RseI CAYNNNNRTG 3 cut(s) 866, 1716, 2044
SacI GAGCTC 1 cut(s) 966
SaqAI TTAA 6 cut(s) 300, 1296, 1329, 1685, 2106, 2145
Sau3AI GATC 9 cut(s) 125, 486, 619, 684, 739, 824, 1261, 1521, 2077
Sau96I GGNCC 3 cut(s) 1352, 1375, 1965
SchI GAGTC 5 cut(s) 566, 803, 945, 1769, 1927
ScrFI CCNGG 3 cut(s) 1652, 1692, 1750
SduI GDGCHC 6 cut(s) 448, 966, 1075, 1950, 2180, 2252
SfaNI GCATC 3 cut(s) 211, 1402, 1968
SfcI CTRYAG 2 cut(s) 414, 1617
SinI GGWCC 2 cut(s) 1375, 1965
SmiMI CAYNNNNRTG 3 cut(s) 866, 1716, 2044
SmlI CTYRAG 6 cut(s) 574, 847, 953, 965, 1328, 1419
SmoI CTYRAG 6 cut(s) 574, 847, 953, 965, 1328, 1419
SpeI ACTAGT 1 cut(s) 325
SphI GCATGC 1 cut(s) 2143
SsiI CCGC 5 cut(s) 167, 371, 762, 1267, 1989
SspI AATATT 1 cut(s) 2275
SspMI CTAG 6 cut(s) 18, 326, 959, 1079, 1244, 1706
SstI GAGCTC 1 cut(s) 966
StyD4I CCNGG 3 cut(s) 1650, 1690, 1748
StyI CCWWGG 2 cut(s) 1078, 1592
TaaI ACNGT 6 cut(s) 115, 463, 605, 1313, 1559, 1679
TaiI ACGT 3 cut(s) 1408, 1429, 2297
TaqI TCGA 7 cut(s) 88, 341, 437, 833, 1567, 1827, 2246
TatI WGTACW 4 cut(s) 984, 1105, 1530, 1672
TauI GCSGC 2 cut(s) 374, 765
TfiI GAWTC 5 cut(s) 633, 773, 1611, 2112, 2286
Tru1I TTAA 6 cut(s) 300, 1296, 1329, 1685, 2106, 2145
Tru9I TTAA 6 cut(s) 300, 1296, 1329, 1685, 2106, 2145
TscAI CASTG 4 cut(s) 120, 466, 772, 2241
TseFI GTSAC 2 cut(s) 1679, 1780
TseI GCWGC 8 cut(s) 1133, 1154, 1301, 1415, 1461, 1597, 1616, 1805
Tsp45I GTSAC 2 cut(s) 1679, 1780
TspDTI ATGAA 7 cut(s) 17, 159, 654, 850, 884, 1250, 2219
TspRI CASTG 4 cut(s) 120, 466, 772, 2241
Tth111I GACNNNGTC 1 cut(s) 1778
Van91I CCANNNNNTGG 1 cut(s) 1511
Vha464I CTTAAG 1 cut(s) 1328
VpaK11BI GGWCC 2 cut(s) 1375, 1965
XagI CCTNNNNNAGG 2 cut(s) 1082, 1649
XapI RAATTY 2 cut(s) 67, 1195
XbaI TCTAGA 1 cut(s) 17
XceI RCATGY 2 cut(s) 748, 2143
XcmI CCANNNNNNNNNTGG 2 cut(s) 1590, 1921
XmaJI CCTAGG 1 cut(s) 1078
XspI CTAG 6 cut(s) 18, 326, 959, 1079, 1244, 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.