MD02G1258200.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000353
Physical Location & Seq
Reverse (-)
31156521 .. 31159971
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1258200.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGGGAAGAGGGACAGTAGAATCGAGAGAGAAGGTGACACTTGCAATAGACAGAGACAAAGGAAGTCAACATGCTATAAAATGGGCCGTTGATAATCTTGTCACCGGAGGCCAACATCTCATCCTGCTCCATGTCAAACAAACGCCACCGGTGGTTTCTGCTGTATCCCAAGGGGAGGAAACTGAATATGTGTACAAAAAACCCGTTGACCCCATAACCAAAGAGCTTTTTCTTCCATTCCGTTCCTTTTGCAGCAAAAAGCTTATAAAATGCAAAGAAGTCGTGGTTGAGGCTGCCGATGTACCAAAAACCTTGATCAACTATGTTATCTCTAATTCAATTGAGGTTTTGGTACTTGGAGCACCCACAAGAACCGGCTTTTTCAATTTAAACAACATTACTGATGATCTTAATGTCGTCCACTCTTTGCTCGAAAGCAACAGATCATTCAAAACAACAACAGACGTCCCAAGCTCTGTGTTAAAAGGGATACCAGATTTCTGCACTCTCTATGTCATTGGGAAAGGAAAGATCTCATATGTGCAAACTGCTACCACAGCACCACCCCAAAAAGCACATAACCAAATTCAAAAACATTCCAGCAGAAATTCGGAAAATGGTGAACAATCCATGCGTAAGCAGCATCCCAGGGGTAAATTTGTTTCTGTTATTCTACAACTGATCAAATCACCATTCACAAGAGGACGATCTTCAATGAACCACTCATACGACCTCTCACCAGAGAGTAGTGATATATCATTTGTGAGCAGCGAAAGGCCAAGCATTGATCACATGTTTCCTTCCTCGTACAATAGTGTATCATCAGAAATGAGCTCCCGACAATCAATGGGCTCGGAGTTTGACAGCATAAGCTCTGCGTCATCTTACTCTAGACACCAATATATTGACATGAGCGCCTCACCATATGGATTCTCAACATCCTCATCAGAAAGTGGAAGATCATGGTCATCGTCACAAAACACAGATGAAGTGGAAGTTGAGATGAGGAGGCTCCAGCTAGAGCTCAAGCAAACAATGGACATGTATAACGCGGCCTGCAAGGAGGCAGTCGCATCAAAACATACGGCAAACCGATTGAAATCGGAAGAACAACAACGATTACATGAGGCACGAATAGCAGAGGAAGCTGCATTGGCACAAATAGAGAATGAGAAAGCGAAATGTAAGGCAGCCATTCAGGCGGCAGCGGCAGCTCAAATGATTGCTGAACTGGAATCCCAAAAAAGGAGGCTTGCAGAAATGACAATTCTTGAACAAGCTGAAGAGAAGAAGGGAACAGAAGCAACGGCATACAGTTTCAGGTACAGGAAATATACAATTGAGGAGATTGAAGTAGCAACAAATAAATTTTCACAAGATCGCAAGATTGGAGAAGGAGGCTATGGACCAGTGTACTGGGGAGAACTGGATCACACACAAGTAGCAATCAAAATTCTACGTCCAGATGCAGCGCACGGACGTTCACAGTTCCAACAAGAGATCGAAGTATTGAGCTGCATACGACATCCAAACATGGTGCTCCTTCTTGGAGCCTGCCCAGAGTATGGTTGTCTGGTCTATGAGTACATGGCTAAGGGGAGCTTAGAAGACCGTCTCTTCCAGCAAGGTAACAGTAAGACCCCGGCTATTCCTTGGCAGCTCAGGTTCCGAATTGCTGCAGAAATTGGCACTGCACTTTTGTTCCTTCACCAGACGAAGCCAGAACCAATTGTACACCGTGACCTAAAACCTGGAAACATTTTGCTAGACCATAACTATGTGAGCAAGATTAGTGATGTAGGTCTAGCCAGGCTTGTCCCTCCATCTGTACAAGACTCTGTCACTCAGTACCGAATGACATCAACAGCCGGAACCTTCTGTTATATCGACCCAGAGTATCAACAAACTGGCATGCTTGGAACAAAATCTGATGTCTACTCACTCGGGGTCATGTTTCTACAACTAATAACAGCGAAATCGCCAATGGGTTTGACTCATCATGTCGAGCAGGCTTTGGAGCTAGGGACCTTTGCTGAGTTGCTAGACCCTGCTGTTCCTGACTGGCCAGTGGAGGAAGCCACCAACTTTGCCAAGTTAGCCTTACAATGTACTGAAATGAGGCGGAAAGACAGACCCGATCTTGGGAAGGTTGTGCTACCTGAACTTAACAGATTGAGAGCATTAGCCGAAGATAGCATGCCTGGTGCTGGTCCTGGTGGAGTATACTCACAAAGACATGGCCAAAGCTCGATGTCCTCATGTGATCAAGTGAGTTGTTTAACATACATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

765

Amino Acids

85.01

Weight (kDa)

6.62

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 10 - 127 2.8e-07 Universal stress protein family
Pkinase PF00069 458 - 713 1.4e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 459 - 717 2.3e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 266
AatII GACGTC 1 cut(s) 468
AccB7I CCANNNNNTGG 1 cut(s) 1565
AccI GTMKAC 2 cut(s) 1935, 2223
AccII CGCG 1 cut(s) 1052
AciI CCGC 4 cut(s) 1052, 1202, 1208, 2122
AclWI GGATC 1 cut(s) 1437
AcoI YGGCCR 2 cut(s) 2063, 2239
AcsI RAATTY 5 cut(s) 585, 607, 656, 1367, 1452
AcuI CTGAAG 1 cut(s) 1302
AcyI GRCGYC 1 cut(s) 465
AfiI CCNNNNNNNGG 6 cut(s) 175, 1245, 1565, 2141, 2142, 2207
AflIII ACRYGT 2 cut(s) 792, 1041
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 8 cut(s) 339, 385, 451, 590, 714, 1099, 1274, 1352
AjnI CCWGG 5 cut(s) 647, 1750, 1808, 2200, 2212
Alw21I GWGCWC 4 cut(s) 364, 836, 1026, 1542
Alw26I GTCTC 2 cut(s) 48, 1619
AlwI GGATC 1 cut(s) 1437
Ama87I CYCGRG 1 cut(s) 1943
AoxI GGCC 6 cut(s) 84, 109, 776, 1053, 2063, 2239
ApoI RAATTY 5 cut(s) 585, 607, 656, 1367, 1452
AsiGI ACCGGT 1 cut(s) 148
AspLEI GCGC 2 cut(s) 917, 1474
AspS9I GGNCC 4 cut(s) 84, 1406, 2025, 2210
AsuC2I CCSGG 1 cut(s) 1643
AsuHPI GGTGA 7 cut(s) 46, 94, 632, 681, 729, 912, 1700
AvaI CYCGRG 1 cut(s) 1943
AvaII GGWCC 3 cut(s) 1406, 2025, 2210
BalI TGGCCA 2 cut(s) 2065, 2241
BanII GRGCYC 3 cut(s) 836, 854, 1026
BbsI GAAGAC 1 cut(s) 1614
Bbv12I GWGCWC 4 cut(s) 364, 836, 1026, 1542
BccI CCATC 1 cut(s) 1831
BceAI ACGGC 3 cut(s) 71, 1101, 1323
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 5 cut(s) 649, 1752, 1810, 2202, 2214
BciVI GTATCC 2 cut(s) 175, 483
BclI TGATCA 4 cut(s) 315, 681, 787, 2263
BcnI CCSGG 1 cut(s) 1643
BcoDI GTCTC 2 cut(s) 48, 1619
BfaI CTAG 7 cut(s) 891, 1019, 1766, 1805, 2021, 2042, 2293
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 918
BfuI GTATCC 2 cut(s) 175, 483
BglI GCCNNNNNGGC 1 cut(s) 1199
BglII AGATCT 1 cut(s) 531
Bme1390I CCNGG 6 cut(s) 649, 1643, 1752, 1810, 2202, 2214
Bme18I GGWCC 3 cut(s) 1406, 2025, 2210
BmeT110I CYCGRG 1 cut(s) 1943
BmgT120I GGNCC 4 cut(s) 84, 1406, 2025, 2210
BmiI GGNNCC 5 cut(s) 1013, 1552, 1667, 1873, 2026
BmrFI CCNGG 6 cut(s) 649, 1643, 1752, 1810, 2202, 2214
BmrI ACTGGG 1 cut(s) 1426
BmsI GCATC 3 cut(s) 652, 1082, 1456
BmuI ACTGGG 1 cut(s) 1426
BpiI GAAGAC 1 cut(s) 1614
BpmI CTGGAG 1 cut(s) 998
Bpu10I CCTNAGC 2 cut(s) 1593, 1661
BpuEI CTTGAG 1 cut(s) 1010
BpuMI CCSGG 1 cut(s) 1643
BsaBI GATNNNNATC 1 cut(s) 1099
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 5 cut(s) 169, 647, 648, 1641, 1652
BsaWI WCCGGW 2 cut(s) 104, 148
Bsc4I CCNNNNNNNGG 6 cut(s) 175, 1245, 1565, 2141, 2142, 2207
Bse118I RCCGGY 2 cut(s) 148, 374
Bse1I ACTGG 7 cut(s) 1236, 1409, 1421, 1431, 1912, 2066, 2066
Bse8I GATNNNNATC 1 cut(s) 1099
BseBI CCWGG 5 cut(s) 649, 1752, 1810, 2202, 2214
BseDI CCNNGG 5 cut(s) 169, 647, 648, 1641, 1652
BseGI GGATG 4 cut(s) 120, 643, 938, 1525
BseJI GATNNNNATC 1 cut(s) 1099
BseLI CCNNNNNNNGG 6 cut(s) 175, 1245, 1565, 2141, 2142, 2207
BseMII CTCAG 3 cut(s) 1675, 1859, 2025
BseNI ACTGG 7 cut(s) 1236, 1409, 1421, 1431, 1912, 2066, 2066
BseRI GAGGAG 2 cut(s) 1021, 1358
BsgI GTGCAG 2 cut(s) 487, 1677
Bsh1236I CGCG 1 cut(s) 1052
BshFI GGCC 6 cut(s) 86, 111, 778, 1055, 2065, 2241
BshTI ACCGGT 1 cut(s) 148
BsiHKAI GWGCWC 4 cut(s) 364, 836, 1026, 1542
BsiHKCI CYCGRG 1 cut(s) 1943
BsiSI CCGG 5 cut(s) 105, 149, 375, 1643, 1869
BslFI GGGAC 4 cut(s) 25, 452, 1802, 2038
BslI CCNNNNNNNGG 6 cut(s) 175, 1245, 1565, 2141, 2142, 2207
BsmAI GTCTC 2 cut(s) 48, 1619
BsmBI CGTCTC 1 cut(s) 1619
BsmFI GGGAC 4 cut(s) 25, 452, 1802, 2038
BsnI GGCC 6 cut(s) 86, 111, 778, 1055, 2065, 2241
BsoBI CYCGRG 1 cut(s) 1943
Bsp1286I GDGCHC 5 cut(s) 364, 836, 854, 1026, 1542
Bsp1407I TGTACA 3 cut(s) 192, 1732, 1828
BspACI CCGC 4 cut(s) 1052, 1202, 1208, 2122
BspANI GGCC 6 cut(s) 86, 111, 778, 1055, 2065, 2241
BspCNI CTCAG 3 cut(s) 1674, 1858, 2026
BspFNI CGCG 1 cut(s) 1052
BspLI GGNNCC 5 cut(s) 1013, 1552, 1667, 1873, 2026
BspMAI CTGCAG 1 cut(s) 1681
BspPI GGATC 1 cut(s) 1437
BsrFI RCCGGY 2 cut(s) 148, 374
BsrGI TGTACA 3 cut(s) 192, 1732, 1828
BsrI ACTGG 7 cut(s) 1236, 1409, 1421, 1431, 1912, 2066, 2066
BssAI RCCGGY 2 cut(s) 148, 374
BssECI CCNNGG 5 cut(s) 169, 647, 648, 1641, 1652
BssNAI GTATAC 1 cut(s) 2224
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 169, 1652
Bst1107I GTATAC 1 cut(s) 2224
Bst2UI CCWGG 5 cut(s) 649, 1752, 1810, 2202, 2214
Bst4CI ACNGT 6 cut(s) 16, 1316, 1488, 1613, 1634, 1739
Bst6I CTCTTC 2 cut(s) 1278, 1622
BstACI GRCGYC 1 cut(s) 465
BstAUI TGTACA 3 cut(s) 192, 1732, 1828
BstC8I GCNNGC 6 cut(s) 1057, 1254, 1555, 1913, 2010, 2198
BstDEI CTNAG 5 cut(s) 1593, 1603, 1661, 1845, 2034
BstF5I GGATG 4 cut(s) 120, 643, 938, 1525
BstFNI CGCG 1 cut(s) 1052
BstH2I RGCGCY 1 cut(s) 918
BstHHI GCGC 2 cut(s) 917, 1474
BstMAI GTCTC 2 cut(s) 48, 1619
BstMWI GCNNNNNNNGC 9 cut(s) 557, 640, 1136, 1145, 1154, 1199, 1208, 1211, 2096
BstNI CCWGG 5 cut(s) 649, 1752, 1810, 2202, 2214
BstNSI RCATGY 5 cut(s) 74, 796, 1045, 1915, 2200
BstSCI CCNGG 6 cut(s) 647, 1641, 1750, 1808, 2200, 2212
BstSFI CTRYAG 1 cut(s) 1677
BstUI CGCG 1 cut(s) 1052
BstV2I GAAGAC 1 cut(s) 1614
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 1416
BstYI RGATCY 1 cut(s) 531
BstZ17I GTATAC 1 cut(s) 2224
BsuI GTATCC 2 cut(s) 175, 483
BsuRI GGCC 6 cut(s) 86, 111, 778, 1055, 2065, 2241
BtsCI GGATG 4 cut(s) 120, 643, 938, 1525
BtsI GCAGTG 1 cut(s) 1689
BtsIMutI CAGTG 3 cut(s) 1416, 1689, 2073
Cac8I GCNNGC 6 cut(s) 1057, 1254, 1555, 1913, 2010, 2198
CfoI GCGC 2 cut(s) 917, 1474
Cfr10I RCCGGY 2 cut(s) 148, 374
Cfr13I GGNCC 4 cut(s) 84, 1406, 2025, 2210
CseI GACGC 1 cut(s) 867
CspAI ACCGGT 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 2068, 2103
DdeI CTNAG 5 cut(s) 1593, 1603, 1661, 1845, 2034
DraI TTTAAA 1 cut(s) 390
EaeI YGGCCR 2 cut(s) 2063, 2239
Eam1104I CTCTTC 2 cut(s) 1278, 1622
EarI CTCTTC 2 cut(s) 1278, 1622
EciI GGCGGA 1 cut(s) 2137
Ecl136II GAGCTC 2 cut(s) 834, 1024
Eco130I CCWWGG 2 cut(s) 169, 1652
Eco24I GRGCYC 3 cut(s) 836, 854, 1026
Eco47I GGWCC 3 cut(s) 1406, 2025, 2210
Eco53kI GAGCTC 2 cut(s) 834, 1024
Eco57I CTGAAG 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 1943
EcoICRI GAGCTC 2 cut(s) 834, 1024
EcoO109I RGGNCCY 1 cut(s) 2025
EcoRII CCWGG 5 cut(s) 647, 1750, 1808, 2200, 2212
EcoT14I CCWWGG 2 cut(s) 169, 1652
EcoT38I GRGCYC 3 cut(s) 836, 854, 1026
ErhI CCWWGG 2 cut(s) 169, 1652
Esp3I CGTCTC 1 cut(s) 1619
FalI AAGNNNNNCTT 4 cut(s) 1586, 1618, 2084, 2116
FaqI GGGAC 4 cut(s) 25, 452, 1802, 2038
FauNDI CATATG 2 cut(s) 538, 925
FbaI TGATCA 4 cut(s) 315, 681, 787, 2263
FblI GTMKAC 2 cut(s) 1935, 2223
FokI GGATG 4 cut(s) 107, 630, 925, 1512
FriOI GRGCYC 3 cut(s) 836, 854, 1026
FspBI CTAG 7 cut(s) 891, 1019, 1766, 1805, 2021, 2042, 2293
GlaI GCGC 2 cut(s) 916, 1473
GsuI CTGGAG 1 cut(s) 998
HaeII RGCGCY 1 cut(s) 918
HaeIII GGCC 6 cut(s) 86, 111, 778, 1055, 2065, 2241
HapII CCGG 5 cut(s) 105, 149, 375, 1643, 1869
HgaI GACGC 1 cut(s) 867
HhaI GCGC 2 cut(s) 917, 1474
Hin1I GRCGYC 1 cut(s) 465
Hin6I GCGC 2 cut(s) 915, 1472
HinP1I GCGC 2 cut(s) 915, 1472
HincII GTYRAC 2 cut(s) 68, 208
HindII GTYRAC 2 cut(s) 68, 208
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 5 cut(s) 20, 930, 1235, 1835, 1993
HpaII CCGG 5 cut(s) 105, 149, 375, 1643, 1869
HphI GGTGA 7 cut(s) 46, 94, 632, 681, 729, 912, 1700
Hpy188I TCNGA 7 cut(s) 613, 826, 856, 949, 1105, 1670, 1930
Hpy188III TCNNGA 6 cut(s) 24, 837, 891, 1271, 1463, 2057
HpyAV CCTTC 8 cut(s) 25, 810, 1285, 1388, 1553, 1715, 1885, 2140
HpyCH4III ACNGT 6 cut(s) 16, 1316, 1488, 1613, 1634, 1739
HpyCH4IV ACGT 3 cut(s) 465, 1459, 1480
HpyF10VI GCNNNNNNNGC 9 cut(s) 557, 640, 1136, 1145, 1154, 1199, 1208, 1211, 2096
HpyF3I CTNAG 5 cut(s) 1593, 1603, 1661, 1845, 2034
HpySE526I ACGT 3 cut(s) 465, 1459, 1480
Hsp92I GRCGYC 1 cut(s) 465
HspAI GCGC 2 cut(s) 915, 1472
Ksp22I TGATCA 4 cut(s) 315, 681, 787, 2263
LmnI GCTCC 8 cut(s) 132, 359, 839, 1017, 1545, 1550, 1599, 2017
LweI GCATC 3 cut(s) 652, 1082, 1456
MaeI CTAG 7 cut(s) 891, 1019, 1766, 1805, 2021, 2042, 2293
MaeII ACGT 3 cut(s) 465, 1459, 1480
MaeIII GTNAC 6 cut(s) 34, 100, 972, 1628, 1739, 1840
MfeI CAATTG 3 cut(s) 339, 1338, 1728
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 5 cut(s) 364, 836, 854, 1026, 1542
MlsI TGGCCA 2 cut(s) 2065, 2241
MluNI TGGCCA 2 cut(s) 2065, 2241
MlyI GAGTC 2 cut(s) 1829, 1987
MmeI TCCRAC 1 cut(s) 1516
Mox20I TGGCCA 2 cut(s) 2065, 2241
MscI TGGCCA 2 cut(s) 2065, 2241
MseI TTAA 5 cut(s) 389, 411, 482, 2166, 2279
MslI CAYNNNNRTG 1 cut(s) 1776
Msp20I TGGCCA 2 cut(s) 2065, 2241
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 5 cut(s) 105, 149, 375, 1643, 1869
MspR9I CCNGG 6 cut(s) 649, 1643, 1752, 1810, 2202, 2214
MunI CAATTG 3 cut(s) 339, 1338, 1728
MvaI CCWGG 5 cut(s) 649, 1752, 1810, 2202, 2214
MvnI CGCG 1 cut(s) 1052
MwoI GCNNNNNNNGC 9 cut(s) 557, 640, 1136, 1145, 1154, 1199, 1208, 1211, 2096
NciI CCSGG 1 cut(s) 1643
NdeI CATATG 2 cut(s) 538, 925
NlaIV GGNNCC 5 cut(s) 1013, 1552, 1667, 1873, 2026
NmuCI GTSAC 5 cut(s) 34, 100, 972, 1739, 1840
NspI RCATGY 5 cut(s) 74, 796, 1045, 1915, 2200
PaeI GCATGC 2 cut(s) 1915, 2200
PasI CCCWGGG 1 cut(s) 648
PciI ACATGT 2 cut(s) 792, 1041
PfeI GAWTC 3 cut(s) 20, 930, 1235
PflFI GACNNNGTC 1 cut(s) 1838
PflMI CCANNNNNTGG 1 cut(s) 1565
PinAI ACCGGT 1 cut(s) 148
PleI GAGTC 2 cut(s) 1829, 1987
PpsI GAGTC 2 cut(s) 1829, 1987
PpuMI RGGWCCY 1 cut(s) 2025
PscI ACATGT 2 cut(s) 792, 1041
PsiI TTATAA 1 cut(s) 266
Psp124BI GAGCTC 2 cut(s) 836, 1026
Psp5II RGGWCCY 1 cut(s) 2025
Psp6I CCWGG 5 cut(s) 647, 1750, 1808, 2200, 2212
PspGI CCWGG 5 cut(s) 647, 1750, 1808, 2200, 2212
PspN4I GGNNCC 5 cut(s) 1013, 1552, 1667, 1873, 2026
PspPI GGNCC 4 cut(s) 84, 1406, 2025, 2210
PspPPI RGGWCCY 1 cut(s) 2025
PsrI GAACNNNNNNTAC 4 cut(s) 710, 742, 1716, 1748
PstI CTGCAG 1 cut(s) 1681
PsuI RGATCY 1 cut(s) 531
PsyI GACNNNGTC 1 cut(s) 1838
RseI CAYNNNNRTG 1 cut(s) 1776
SacI GAGCTC 2 cut(s) 836, 1026
SaqAI TTAA 5 cut(s) 389, 411, 482, 2166, 2279
Sau96I GGNCC 4 cut(s) 84, 1406, 2025, 2210
SchI GAGTC 2 cut(s) 1829, 1987
ScrFI CCNGG 6 cut(s) 649, 1643, 1752, 1810, 2202, 2214
SduI GDGCHC 5 cut(s) 364, 836, 854, 1026, 1542
SfaNI GCATC 3 cut(s) 652, 1082, 1456
SfcI CTRYAG 1 cut(s) 1677
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 3 cut(s) 1406, 2025, 2210
SmiMI CAYNNNNRTG 1 cut(s) 1776
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
SphI GCATGC 2 cut(s) 1915, 2200
SsiI CCGC 4 cut(s) 1052, 1202, 1208, 2122
SspMI CTAG 7 cut(s) 891, 1019, 1766, 1805, 2021, 2042, 2293
SstI GAGCTC 2 cut(s) 836, 1026
StyD4I CCNGG 6 cut(s) 647, 1641, 1750, 1808, 2200, 2212
StyI CCWWGG 2 cut(s) 169, 1652
TaaI ACNGT 6 cut(s) 16, 1316, 1488, 1613, 1634, 1739
TaiI ACGT 3 cut(s) 468, 1462, 1483
TaqI TCGA 6 cut(s) 23, 432, 1503, 1887, 2004, 2249
TatI WGTACW 6 cut(s) 192, 1413, 1584, 1732, 1828, 2108
TauI GCSGC 3 cut(s) 1055, 1205, 1211
TfiI GAWTC 3 cut(s) 20, 930, 1235
Tru1I TTAA 5 cut(s) 389, 411, 482, 2166, 2279
Tru9I TTAA 5 cut(s) 389, 411, 482, 2166, 2279
TscAI CASTG 3 cut(s) 1416, 1696, 2073
TseFI GTSAC 5 cut(s) 34, 100, 972, 1739, 1840
Tsp45I GTSAC 5 cut(s) 34, 100, 972, 1739, 1840
TspDTI ATGAA 2 cut(s) 731, 1002
TspGWI ACGGA 2 cut(s) 230, 1491
TspRI CASTG 3 cut(s) 1416, 1696, 2073
Tth111I GACNNNGTC 1 cut(s) 1838
Van91I CCANNNNNTGG 1 cut(s) 1565
VpaK11BI GGWCC 3 cut(s) 1406, 2025, 2210
XapI RAATTY 5 cut(s) 585, 607, 656, 1367, 1452
XbaI TCTAGA 1 cut(s) 890
XceI RCATGY 5 cut(s) 74, 796, 1045, 1915, 2200
XmiI GTMKAC 2 cut(s) 1935, 2223
XspI CTAG 7 cut(s) 891, 1019, 1766, 1805, 2021, 2042, 2293
ZraI GACGTC 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.