MD09G1226500.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000353
Physical Location & Seq
Reverse (-)
27918973 .. 27923725
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1226500.v1.1.491

Sequence Viewer

Length: 2016 bp
ATGTCGAGGATTGTTAGAGGGCAAGGAGAAAAGAGAGAGGAGTCGGTGGCGGTGGCCATCGACAAGGACAAAGGAAGTGAACATGCAATCAAATGGGCCGTTGATCATCTTCTCGCCAGAGGCCAGCCTCTCACATTGCTCCATATAAAATGCAATGAAGTGGTAATCAAGGACACTGACATTCCGAACGCAATCATCGATTATGTTATGGTTAACACTATCGAGATTTTGGTACTTGGCACACCGTCCAAAAGCGGAATTTTCAGATTTAAGCTCACTGACGTTCCAAGCACAGTGGCGAAAAGGGCACCGGATTTTTGCTCTGTTTATGTGATTGGCAAAGGAAAGGTGTCGTATATGCGATGTGCTACTTCCACACCCCAAAATTTTGCCCAACGTAGTCAAGTTCAACACCAACCAAGCACTGTTTCTGAAGCCAGATCTTCAGCATCGGATAGGATGCAGTACTGTTCTACTCGTAACCAGCCAGATGGTGACATTCAAATCAAGTCACCGTTCGCAAGAGGAAGACCCTCAATGGACAAATCATATGAGCTTTCACCAGAAACTGATATATCATTTGTAAGTAGTGGAAGGCCAAGCATTGATCGTATGTCACCTCGCTGCGACTTTGGAGTAGAATCAGGAATGAACTCTCGACTTTCAATCGGCTCGGACGGGGACAACAGAAGCCCTACATCATCTTACTCAAGACACAAGTTTTTAGATATGGGCTCTTTGCAATGTGAAATGTCATCATGTTCAATTGACAGTGGAAAATCATCGTCATCATCACAAAATATGGACGATGTGGAATCTGAGATGAGGAGGCTCAAGCTAGAGCTGAAACAAACAATGGAAATGTACAGTACAGCATGCAAGGAAGCTCTGATAGAAAAACACAAGGAAGTTGCATTGGCACTCGTGGAGAAGGAGAAAGCAAAATGTAAGGCAGCCATTGAGGCAGCTGAAGCAGCTCAAAGGATTGCTGAATTGGAAGCACAAAAAAGAAGGGTTGCAGAAATGAAAGCTCTTAAAGAATCCGAAGAGAAGAATAAGGCACTTCAAGCAAGGTCATATGATCTTAGGTACAGGAAATACACAATTCAGGAGATTGAAAAGGCAACGAATGATTTCTCACAAGATCGAAATATTGGTGAAGGAGGCTATGGGCCAGTATACAGAGGTGAACTAGACCACACACCAGTGGCGATAAAAATTCTACGTCCAGATGCAGCTGTTGAGGTATTAAGCTGTATACGACATCCAAATACGGTTCTACTCCTTGGAGCTTGCCCAGATTATGGTTGCTTGGTATATGAGTACATGGGTAATGGGACCTTGGAAGATCGCCTCTTCAGCAGAGGTAACACCCCAGTTATTCCTTGGCAGGTAAGGTTCCGAATTGCTGCAGAAATTGGGACAGGACTTTTGTTCCTTCATCAGACCAAGCCAGAACCCCTCGTACATCGTGACCTAAAACCCGGCAACATCTTGCTAGACCATAACTATGTGAGCAAGATCGGTGATGTAGGTTTAGCTAGGCTTGTCCCTCCATCTGTAGCCAACGCCGTCACTCAATATCACATGACATCAACAGCCGGAACATTTTGCTACATCAACCCAGAGTATCAGCAAATTGGCTTGCTTGGGACAAAATCTGATATCTACTCACTTGGTGTCATGTTTTTACAACTTATAACAGCCAAGCCACCAATGGGGTTGACTCATCACGTTGAACAGGCTATTGAGACGGGGACTTTTGTTGAGATGCTTGACCCTGCTGTTCCTGATTGGCCAGTTGAGGAAGCCTTGAAGTTTGCCAAGCTCTGTTTACAGTGTGCTGAAATGAAGCGAAAGGACAGGCCAGATCTTGGCAAGGTTGTGCTGCCTGAACTTAACAGATTGAGAGCACTTGCCGATGAGAGCTTGAATTGTGTCATGCTTGGTGCTACTGGAGTATTCTCACTGAGACAACGCTCGAATCCTTCGATACAAGTGAGTTGCATAACATAA

Protein Analysis

672

Amino Acids

74.44

Weight (kDa)

7.79

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 380 - 619 1e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 381 - 628 3.6e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1700
Acc36I ACCTGC 1 cut(s) 1381
AccB1I GGYRCC 1 cut(s) 307
AccB7I CCANNNNNTGG 2 cut(s) 1304, 1874
AccI GTMKAC 2 cut(s) 1179, 1258
AciI CCGC 2 cut(s) 50, 255
AcoI YGGCCR 2 cut(s) 54, 1796
AcsI RAATTY 3 cut(s) 258, 385, 1218
AcuI CTGAAG 4 cut(s) 429, 453, 990, 1342
AdeI CACNNNGTG 1 cut(s) 1679
AfaI GTAC 7 cut(s) 234, 467, 866, 871, 1091, 1325, 1467
AfiI CCNNNNNNNGG 3 cut(s) 1304, 1718, 1874
AgsI TTSAA 9 cut(s) 410, 503, 666, 765, 1067, 1118, 1739, 1816, 1933
AjuI GAANNNNNNNTTGG 2 cut(s) 412, 444
AleI CACNNNNGTG 1 cut(s) 1205
Alw21I GWGCWC 1 cut(s) 1915
Alw26I GTCTC 2 cut(s) 1745, 1966
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 7 cut(s) 54, 96, 121, 596, 1172, 1796, 1865
ApeKI GCWGC 7 cut(s) 624, 953, 965, 974, 1235, 1409, 1888
ApoI RAATTY 3 cut(s) 258, 385, 1218
ArsI GACNNNNNNTTYG 2 cut(s) 1414, 1446
Asp700I GAANNNNTTC 1 cut(s) 1133
AspS9I GGNCC 3 cut(s) 96, 1172, 1338
AsuC2I CCSGG 1 cut(s) 1485
AsuHPI GGTGA 7 cut(s) 504, 506, 552, 609, 1169, 1199, 1538
AvaII GGWCC 1 cut(s) 1338
BaeGI GKGCMC 1 cut(s) 310
BalI TGGCCA 2 cut(s) 56, 1798
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 737
BauI CACGAG 1 cut(s) 923
BbsI GAAGAC 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 1915
BbvI GCAGC 7 cut(s) 611, 965, 977, 986, 1247, 1396, 1875
BccI CCATC 3 cut(s) 65, 485, 1564
BceAI ACGGC 2 cut(s) 83, 1556
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 1 cut(s) 1485
BcoDI GTCTC 2 cut(s) 1745, 1966
BfaI CTAG 4 cut(s) 839, 1193, 1499, 1542
BfmI CTRYAG 2 cut(s) 1410, 1560
BfuAI ACCTGC 1 cut(s) 1381
BglI GCCNNNNNGGC 1 cut(s) 962
BglII AGATCT 2 cut(s) 440, 1870
BisI GCNGC 7 cut(s) 625, 954, 966, 975, 1236, 1410, 1889
BlsI GCNGC 7 cut(s) 626, 955, 967, 976, 1237, 1411, 1890
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 1 cut(s) 1485
Bme18I GGWCC 1 cut(s) 1338
BmgT120I GGNCC 3 cut(s) 96, 1172, 1338
BmiI GGNNCC 3 cut(s) 309, 1339, 1400
BmrFI CCNGG 1 cut(s) 1485
BmrI ACTGGG 1 cut(s) 1370
BmsI GCATC 4 cut(s) 450, 458, 1222, 1761
BmuI ACTGGG 1 cut(s) 1370
BpiI GAAGAC 1 cut(s) 535
BpmI CTGGAG 1 cut(s) 1977
BpuEI CTTGAG 2 cut(s) 694, 818
BpuMI CCSGG 1 cut(s) 1485
Bsa29I ATCGAT 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 1285, 1341, 1385
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 3 cut(s) 1304, 1718, 1874
Bse1I ACTGG 5 cut(s) 1175, 1205, 1376, 1799, 1960
Bse3DI GCAATG 3 cut(s) 134, 160, 749
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 1285, 1341, 1385
BseGI GGATG 2 cut(s) 465, 1264
BseLI CCNNNNNNNGG 3 cut(s) 1304, 1718, 1874
BseMI GCAATG 3 cut(s) 134, 160, 749
BseMII CTCAG 2 cut(s) 810, 1961
BseNI ACTGG 5 cut(s) 1175, 1205, 1376, 1799, 1960
BseRI GAGGAG 2 cut(s) 53, 841
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 7 cut(s) 611, 965, 977, 986, 1247, 1396, 1875
BshFI GGCC 7 cut(s) 56, 98, 123, 598, 1174, 1798, 1867
BshNI GGYRCC 1 cut(s) 307
BshVI ATCGAT 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 1915
BsiSI CCGG 3 cut(s) 311, 1485, 1602
BslFI GGGAC 6 cut(s) 695, 1351, 1435, 1535, 1666, 1771
BslI CCNNNNNNNGG 3 cut(s) 1304, 1718, 1874
BsmAI GTCTC 2 cut(s) 1745, 1966
BsmBI CGTCTC 1 cut(s) 1745
BsmFI GGGAC 6 cut(s) 695, 1351, 1435, 1535, 1666, 1771
BsnI GGCC 7 cut(s) 56, 98, 123, 598, 1174, 1798, 1867
Bsp1286I GDGCHC 3 cut(s) 310, 737, 1915
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
BspACI CCGC 2 cut(s) 50, 255
BspANI GGCC 7 cut(s) 56, 98, 123, 598, 1174, 1798, 1867
BspCNI CTCAG 2 cut(s) 811, 1962
BspDI ATCGAT 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 309, 1339, 1400
BspMAI CTGCAG 1 cut(s) 1414
BspMI ACCTGC 1 cut(s) 1381
BspT107I GGYRCC 1 cut(s) 307
BsrDI GCAATG 3 cut(s) 134, 160, 749
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 5 cut(s) 1175, 1205, 1376, 1799, 1960
BssECI CCNNGG 3 cut(s) 1285, 1341, 1385
BssMI GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
BssNAI GTATAC 2 cut(s) 1180, 1259
BssSI CACGAG 1 cut(s) 923
BssT1I CCWWGG 3 cut(s) 1285, 1341, 1385
Bst1107I GTATAC 2 cut(s) 1180, 1259
Bst2BI CACGAG 1 cut(s) 923
Bst4CI ACNGT 9 cut(s) 246, 295, 427, 470, 516, 773, 869, 1276, 1839
Bst6I CTCTTC 2 cut(s) 1041, 1361
BstAUI TGTACA 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 125, 877, 1294, 1646
BstDEI CTNAG 3 cut(s) 819, 1085, 1970
BstF5I GGATG 2 cut(s) 465, 1264
BstKTI GATC 8 cut(s) 106, 443, 610, 1084, 1147, 1351, 1524, 1873
BstMAI GTCTC 2 cut(s) 1745, 1966
BstMBI GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
BstMWI GCNNNNNNNGC 6 cut(s) 305, 962, 971, 974, 1067, 1359
BstNSI RCATGY 2 cut(s) 86, 879
BstSCI CCNGG 1 cut(s) 1483
BstSFI CTRYAG 2 cut(s) 1410, 1560
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 7 cut(s) 611, 965, 977, 986, 1247, 1396, 1875
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 2 cut(s) 440, 1870
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 2 cut(s) 440, 1870
BstZ17I GTATAC 2 cut(s) 1180, 1259
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 7 cut(s) 56, 98, 123, 598, 1174, 1798, 1867
BsuTUI ATCGAT 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 2 cut(s) 465, 1264
BtsIMutI CAGTG 8 cut(s) 174, 276, 300, 423, 778, 1212, 1844, 1967
BveI ACCTGC 1 cut(s) 1381
Cac8I GCNNGC 4 cut(s) 125, 877, 1294, 1646
CaiI CAGNNNCTG 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 96, 1172, 1338
ClaI ATCGAT 1 cut(s) 198
Csp6I GTAC 7 cut(s) 233, 466, 865, 870, 1090, 1324, 1466
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 7 cut(s) 83, 759, 876, 1327, 1588, 1684, 1942
CviQI GTAC 7 cut(s) 233, 466, 865, 870, 1090, 1324, 1466
DdeI CTNAG 3 cut(s) 819, 1085, 1970
DpnI GATC 8 cut(s) 105, 442, 609, 1083, 1146, 1350, 1523, 1872
DpnII GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
DraIII CACNNNGTG 1 cut(s) 1679
EaeI YGGCCR 2 cut(s) 54, 1796
Eam1104I CTCTTC 2 cut(s) 1041, 1361
EarI CTCTTC 2 cut(s) 1041, 1361
Eco130I CCWWGG 3 cut(s) 1285, 1341, 1385
Eco24I GRGCYC 1 cut(s) 737
Eco32I GATATC 1 cut(s) 1666
Eco47I GGWCC 1 cut(s) 1338
Eco57I CTGAAG 4 cut(s) 429, 453, 990, 1342
EcoO109I RGGNCCY 1 cut(s) 1338
EcoRV GATATC 1 cut(s) 1666
EcoT14I CCWWGG 3 cut(s) 1285, 1341, 1385
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 3 cut(s) 1285, 1341, 1385
Esp3I CGTCTC 1 cut(s) 1745
FaeI CATG 7 cut(s) 86, 762, 879, 1330, 1591, 1687, 1945
FaqI GGGAC 6 cut(s) 695, 1351, 1435, 1535, 1666, 1771
FatI CATG 7 cut(s) 82, 758, 875, 1326, 1587, 1683, 1941
FauNDI CATATG 2 cut(s) 550, 1078
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 2 cut(s) 1179, 1258
Fnu4HI GCNGC 7 cut(s) 625, 954, 966, 975, 1236, 1410, 1889
FokI GGATG 2 cut(s) 472, 1251
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 7 cut(s) 625, 954, 966, 975, 1236, 1410, 1889
FspBI CTAG 4 cut(s) 839, 1193, 1499, 1542
GluI GCNGC 7 cut(s) 625, 954, 966, 975, 1236, 1410, 1889
GsuI CTGGAG 1 cut(s) 1977
HaeIII GGCC 7 cut(s) 56, 98, 123, 598, 1174, 1798, 1867
HapII CCGG 3 cut(s) 311, 1485, 1602
Hin1II CATG 7 cut(s) 86, 762, 879, 1330, 1591, 1687, 1945
HincII GTYRAC 2 cut(s) 214, 1725
HindII GTYRAC 2 cut(s) 214, 1725
HinfI GANTC 6 cut(s) 41, 641, 815, 1040, 1726, 1984
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 3 cut(s) 311, 1485, 1602
HphI GGTGA 7 cut(s) 504, 506, 552, 609, 1169, 1199, 1538
Hpy166II GTNNAC 7 cut(s) 80, 214, 1180, 1190, 1259, 1725, 1835
Hpy188III TCNNGA 8 cut(s) 223, 645, 657, 711, 1109, 1229, 1472, 1790
Hpy8I GTNNAC 7 cut(s) 80, 214, 1180, 1190, 1259, 1725, 1835
HpyAV CCTTC 6 cut(s) 588, 925, 1005, 1154, 1448, 1998
HpyCH4III ACNGT 9 cut(s) 246, 295, 427, 470, 516, 773, 869, 1276, 1839
HpyCH4IV ACGT 4 cut(s) 282, 397, 1225, 1734
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 962, 971, 974, 1067, 1359
HpyF3I CTNAG 3 cut(s) 819, 1085, 1970
HpySE526I ACGT 4 cut(s) 282, 397, 1225, 1734
Hsp92II CATG 7 cut(s) 86, 762, 879, 1330, 1591, 1687, 1945
Ksp22I TGATCA 1 cut(s) 103
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
LmnI GCTCC 2 cut(s) 144, 1289
Lsp1109I GCAGC 7 cut(s) 611, 965, 977, 986, 1247, 1396, 1875
LweI GCATC 4 cut(s) 450, 458, 1222, 1761
MaeI CTAG 4 cut(s) 839, 1193, 1499, 1542
MaeII ACGT 4 cut(s) 282, 397, 1225, 1734
MaeIII GTNAC 7 cut(s) 479, 494, 510, 615, 1367, 1472, 1573
MalI GATC 8 cut(s) 105, 442, 609, 1083, 1146, 1350, 1523, 1872
MboI GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
MboII GAAGA 7 cut(s) 101, 435, 540, 1058, 1063, 1348, 1358
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 2 cut(s) 440, 1870
MhlI GDGCHC 3 cut(s) 310, 737, 1915
MlsI TGGCCA 2 cut(s) 56, 1798
MluNI TGGCCA 2 cut(s) 56, 1798
MlyI GAGTC 2 cut(s) 50, 1720
Mox20I TGGCCA 2 cut(s) 56, 1798
MroXI GAANNNNTTC 1 cut(s) 1133
MscI TGGCCA 2 cut(s) 56, 1798
MseI TTAA 5 cut(s) 213, 270, 1035, 1250, 1899
MslI CAYNNNNRTG 2 cut(s) 1205, 1509
Msp20I TGGCCA 2 cut(s) 56, 1798
MspA1I CMGCKG 2 cut(s) 968, 1238
MspI CCGG 3 cut(s) 311, 1485, 1602
MspR9I CCNGG 1 cut(s) 1485
MunI CAATTG 1 cut(s) 765
MwoI GCNNNNNNNGC 6 cut(s) 305, 962, 971, 974, 1067, 1359
NciI CCSGG 1 cut(s) 1485
NdeI CATATG 2 cut(s) 550, 1078
NdeII GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
NlaIII CATG 7 cut(s) 86, 762, 879, 1330, 1591, 1687, 1945
NlaIV GGNNCC 3 cut(s) 309, 1339, 1400
NmuCI GTSAC 5 cut(s) 494, 510, 615, 1472, 1573
NspI RCATGY 2 cut(s) 86, 879
OliI CACNNNNGTG 1 cut(s) 1205
PaeI GCATGC 1 cut(s) 879
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 1988
PdmI GAANNNNTTC 1 cut(s) 1133
PfeI GAWTC 4 cut(s) 641, 815, 1040, 1984
PflMI CCANNNNNTGG 2 cut(s) 1304, 1874
PkrI GCNGC 7 cut(s) 626, 955, 967, 976, 1237, 1411, 1890
PleI GAGTC 2 cut(s) 49, 1720
PpsI GAGTC 2 cut(s) 49, 1720
PpuMI RGGWCCY 1 cut(s) 1338
PsiI TTATAA 1 cut(s) 1700
Psp5II RGGWCCY 1 cut(s) 1338
PspN4I GGNNCC 3 cut(s) 309, 1339, 1400
PspPI GGNCC 3 cut(s) 96, 1172, 1338
PspPPI RGGWCCY 1 cut(s) 1338
PsrI GAACNNNNNNTAC 2 cut(s) 1449, 1481
PstI CTGCAG 1 cut(s) 1414
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 2 cut(s) 440, 1870
PvuII CAGCTG 2 cut(s) 968, 1238
RsaI GTAC 7 cut(s) 234, 467, 866, 871, 1091, 1325, 1467
RsaNI GTAC 7 cut(s) 233, 466, 865, 870, 1090, 1324, 1466
RseI CAYNNNNRTG 2 cut(s) 1205, 1509
SaqAI TTAA 5 cut(s) 213, 270, 1035, 1250, 1899
SatI GCNGC 7 cut(s) 625, 954, 966, 975, 1236, 1410, 1889
Sau3AI GATC 8 cut(s) 103, 440, 607, 1081, 1144, 1348, 1521, 1870
Sau96I GGNCC 3 cut(s) 96, 1172, 1338
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 2 cut(s) 50, 1720
ScrFI CCNGG 1 cut(s) 1485
SduI GDGCHC 3 cut(s) 310, 737, 1915
SfaNI GCATC 4 cut(s) 450, 458, 1222, 1761
SfcI CTRYAG 2 cut(s) 1410, 1560
SinI GGWCC 1 cut(s) 1338
SmiMI CAYNNNNRTG 2 cut(s) 1205, 1509
SmlI CTYRAG 2 cut(s) 709, 833
SmoI CTYRAG 2 cut(s) 709, 833
SphI GCATGC 1 cut(s) 879
SsiI CCGC 2 cut(s) 50, 255
SspI AATATT 1 cut(s) 1153
SspMI CTAG 4 cut(s) 839, 1193, 1499, 1542
StyD4I CCNGG 1 cut(s) 1483
StyI CCWWGG 3 cut(s) 1285, 1341, 1385
TaaI ACNGT 9 cut(s) 246, 295, 427, 470, 516, 773, 869, 1276, 1839
TaiI ACGT 4 cut(s) 285, 400, 1228, 1737
TaqI TCGA 8 cut(s) 5, 60, 198, 222, 658, 1147, 1982, 1991
TatI WGTACW 4 cut(s) 465, 864, 869, 1323
TfiI GAWTC 4 cut(s) 641, 815, 1040, 1984
Tru1I TTAA 5 cut(s) 213, 270, 1035, 1250, 1899
Tru9I TTAA 5 cut(s) 213, 270, 1035, 1250, 1899
TscAI CASTG 8 cut(s) 181, 283, 300, 430, 778, 1212, 1844, 1974
TseFI GTSAC 5 cut(s) 494, 510, 615, 1472, 1573
TseI GCWGC 7 cut(s) 624, 953, 965, 974, 1235, 1409, 1888
Tsp45I GTSAC 5 cut(s) 494, 510, 615, 1472, 1573
TspDTI ATGAA 5 cut(s) 171, 665, 1040, 1430, 1865
TspRI CASTG 8 cut(s) 181, 283, 300, 430, 778, 1212, 1844, 1974
Van91I CCANNNNNTGG 2 cut(s) 1304, 1874
VpaK11BI GGWCC 1 cut(s) 1338
XapI RAATTY 3 cut(s) 258, 385, 1218
XceI RCATGY 2 cut(s) 86, 879
XcmI CCANNNNNNNNNTGG 2 cut(s) 1383, 1714
XmiI GTMKAC 2 cut(s) 1179, 1258
XmnI GAANNNNTTC 1 cut(s) 1133
XspI CTAG 4 cut(s) 839, 1193, 1499, 1542
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.