RLG00000019447

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000353
Physical Location & Seq
Forward (+)
52119102 .. 52122734
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019447

Sequence Viewer

Length: 2127 bp
ATGGGACTAAGAAGGCAAGCATCCAGAAACAGAGGTGATGCAGTAGCAATGGCAATAGACAAGGACAAAGGAAGTCAATATGCTTTCCAATGGGTTGTTAATCATCTCGTCAAGCCAGGCCAAACTCTCACATTGCTTCATGTTAAGCAACCTGCAGCTCCCAACCAAACAGGGCGCATGGTTCCTAGCTCTGAAGTGACCCAGGAGGTTGCAGAAATCAAAAACCAACCTGATGCTCAAGCCAAAGTGTGGCTTCATCCAATGCATTGTGTTTGCATCAAAAAGAAAATAACCTGCAATGAAGTCATACTAGAAGATTCTGATATACCAAAATCGCTTATTAACTATGTCGCGGCAAATTCAATCGAGGTTTTGGTACTTGGTGTACCATCTAAAAGCAGCCTTTTCAGATTCAAAACTAGAGACATACCGAGCACCGTGTTGAGGGGGGCACCGGACTTTTGCACTGTATATGTTATTGGCAGAGAAAAGATCTCGTCTGTGCGATCTGCAACTGGTGCTCTACCCCAAAAACCAAAATGGCTTCCATCTCCATGTGAACAAAATGTGCAGCATCACCAAAGCGTTTCGAATTCTTTGATGAATGCACCATCAGAAAGGACACAGTCTCCGCAGTGTAGCCTGCCAAATGACACTAAACTCGAGTCAGCAACTATATTACCGGGGAGCAGTAACAAGCAAAGCTTGGGTCGCACCTTTGCTTTCTCTGATAGCATGACAGCAGGAATGCCCCCTAGGCATCCAACTAGCTCAGAGTCAGACAGAAGCTCTACATCGACAGCTTACTCCGGAATGACATCTTACTCCGGAACCGAATTTATTGACATGACCTGTCCACAGAATGAATTTTCATCTTCCCCAACAGAAAGTGGAAGGTTATCAACATCACAGAACATGGATGGAATGGAAGCTGAGCTGAGGAGGCTTCAGTTAGAGCTCAAGCAAACAATGGACATGTATAATATAGCCTGCAAAGAGGCAATCACAGAAAAAAATAAGGCAAAGGAACTTAACCTATGGAAATTGGAAAAAGAATATAAATTAGAAGAGGCACGATTAGCTGAGGAAATGGCATTGAAACTAGCGGAGAAAGAGAAAGAAAAATGTAAGGCAGCTATCAAGGAAGCAGAAGCAGCTCAAAGGGTTGCCAGATTGGAAGCACAAAGAGCGAGGATTGCAGAAATGGAAGCTCTGAAACAAGCAGAGGCGAAGAAGGTATCAGAAGCAATGACATATGATCTAAGGTACAGGAAATACACAATTGAGGAGCTTGAAGCAGCAACAAATGGTTTTTCACAAGCTTGCAAAATTGGGGAAGGAGGCTATGGGCCAGTATATCTGGGTGACATAGACCATACACGAGTGGCAATAAAAGTTCTGCGTCCTGATGCAGCTCAAGGACGGTTGCAGTTCCAGAAAGAGGTTGAAATATTGAGCTGCATACGGCATCCAAACATGGTTCTCCTTCTTGGAGCCTGCCCTGAGTATGGTTGCCTGGTCTACGAGTACATGGCTAATGGTAGCTTAGAGGATCGTCTCTTCCGGCGATGTAACAAACGAGTTATTCCTTGGCAGCTAAGGTTCAGAATTGCTGCTGATATCTGCACTGGGCTTTTGTTTCTTCATCAAACTAAGCCGGAACCCCTAGTACACCGTGATCTCAAACCCGGAAACATTTTGCTAGACCGTAACTATGTTAGCAAGATAAGTGATGTAGGTTTGGCCAGGCTTGTCCCTCAATCAGTAGCTGATTCGGTCACTCAGTATTGCATGACAGCAACAGCAGGAACATTCTGTTATATCGATCCTGAGTATCAGCAAACAGGCATGCTTGTAACAAAGTCTGATGTCTACTCACTTGGTGTCATTCTTTTACAACTAATAACAGCCAAGCCACCAATGGGTCTGACTCTTCAAGTTGAGAAAGCTATTGAGATAGGGAACTTTGCTCAAATCCTTGACCCTGCTGTTCCTGACTGGCCTGTTGAAGAAGCCTTAAAGTTTGCCAAACTAGGTTTACAGTGTGCAGAAATGAGGCGGAAAGACAGACCGGATCTTGGGAAGGTTGTGCTACCTGAGCTCAATAGATTGAGTGCACTTAGATAG

Protein Analysis

709

Amino Acids

78.85

Weight (kDa)

8.55

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 15 - 152 9.7e-08 Universal stress protein family
Pkinase PF00069 440 - 691 1.7e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 441 - 696 1.9e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 160, 302
AccB1I GGYRCC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 249
AccI GTMKAC 2 cut(s) 1521, 1872
AccII CGCG 1 cut(s) 353
AccIII TCCGGA 2 cut(s) 809, 827
AciI CCGC 4 cut(s) 353, 632, 1106, 2059
AclWI GGATC 3 cut(s) 1560, 1820, 2082
AcoI YGGCCR 1 cut(s) 1743
AcsI RAATTY 4 cut(s) 358, 592, 836, 866
AcuI CTGAAG 2 cut(s) 213, 932
AdeI CACNNNGTG 1 cut(s) 1883
AfaI GTAC 5 cut(s) 378, 387, 1268, 1529, 1671
AfiI CCNNNNNNNGG 6 cut(s) 249, 444, 1441, 1508, 1922, 2078
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 7 cut(s) 363, 415, 1099, 1295, 1448, 1937, 2009
AjnI CCWGG 4 cut(s) 115, 201, 1515, 1745
Alw21I GWGCWC 5 cut(s) 437, 523, 960, 2103, 2119
Alw26I GTCTC 3 cut(s) 417, 633, 1562
Alw44I GTGCAC 1 cut(s) 2115
AlwI GGATC 3 cut(s) 1560, 1820, 2082
AlwNI CAGNNNCTG 1 cut(s) 1769
Ama87I CYCGRG 1 cut(s) 662
Aor13HI TCCGGA 2 cut(s) 809, 827
AoxI GGCC 4 cut(s) 118, 1349, 1743, 2000
ApaLI GTGCAC 1 cut(s) 2115
ApoI RAATTY 4 cut(s) 358, 592, 836, 866
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
AspA2I CCTAGG 1 cut(s) 755
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 1349
AsuC2I CCSGG 2 cut(s) 684, 1689
AsuHPI GGTGA 3 cut(s) 47, 569, 1376
AsuII TTCGAA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 662
AvrII CCTAGG 1 cut(s) 755
BaeGI GKGCMC 2 cut(s) 454, 2119
BalI TGGCCA 1 cut(s) 1745
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 2 cut(s) 960, 2103
BauI CACGAG 1 cut(s) 1380
Bbv12I GWGCWC 5 cut(s) 437, 523, 960, 2103, 2119
BbvCI CCTCAGC 2 cut(s) 938, 1083
BccI CCATC 4 cut(s) 397, 556, 619, 914
BceAI ACGGC 1 cut(s) 1481
BciT130I CCWGG 4 cut(s) 117, 203, 1517, 1747
BcnI CCSGG 2 cut(s) 684, 1689
BcoDI GTCTC 3 cut(s) 417, 633, 1562
BfaI CTAG 9 cut(s) 186, 311, 420, 756, 768, 1103, 1667, 1703, 2033
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 160, 302
BglI GCCNNNNNGGC 1 cut(s) 757
BglII AGATCT 1 cut(s) 492
BlnI CCTAGG 1 cut(s) 755
BlpI GCTNAGC 1 cut(s) 933
Bme1390I CCNGG 6 cut(s) 117, 203, 684, 1517, 1689, 1747
BmeT110I CYCGRG 1 cut(s) 662
BmgT120I GGNCC 1 cut(s) 1349
BmiI GGNNCC 5 cut(s) 183, 453, 832, 1495, 1662
BmrFI CCNGG 6 cut(s) 117, 203, 684, 1517, 1689, 1747
BmrI ACTGGG 1 cut(s) 1638
BmsI GCATC 8 cut(s) 28, 29, 223, 285, 583, 769, 1399, 1477
BmuI ACTGGG 1 cut(s) 1638
Bpu10I CCTNAGC 4 cut(s) 938, 1083, 1598, 2097
Bpu1102I GCTNAGC 1 cut(s) 933
Bpu14I TTCGAA 1 cut(s) 590
BpuEI CTTGAG 3 cut(s) 222, 944, 1401
BpuMI CCSGG 2 cut(s) 684, 1689
Bsa29I ATCGAT 1 cut(s) 1824
BsaJI CCNNGG 4 cut(s) 201, 683, 755, 1589
BsaWI WCCGGW 4 cut(s) 454, 809, 827, 2071
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 444, 1441, 1508, 1922, 2078
Bse1I ACTGG 4 cut(s) 520, 1352, 1633, 2003
Bse3DI GCAATG 4 cut(s) 54, 131, 304, 1254
BseAI TCCGGA 2 cut(s) 809, 827
BseBI CCWGG 4 cut(s) 117, 203, 1517, 1747
BseCI ATCGAT 1 cut(s) 1824
BseDI CCNNGG 4 cut(s) 201, 683, 755, 1589
BseGI GGATG 5 cut(s) 20, 256, 760, 925, 1468
BseLI CCNNNNNNNGG 6 cut(s) 249, 444, 1441, 1508, 1922, 2078
BseMI GCAATG 4 cut(s) 54, 131, 304, 1254
BseMII CTCAG 8 cut(s) 786, 924, 929, 1074, 1494, 1796, 1821, 2088
BseNI ACTGG 4 cut(s) 520, 1352, 1633, 2003
BseRI GAGGAG 2 cut(s) 955, 1301
BseSI GKGCMC 2 cut(s) 454, 2119
BsgI GTGCAG 3 cut(s) 590, 1609, 2067
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 4 cut(s) 120, 1351, 1745, 2002
BshNI GGYRCC 1 cut(s) 451
BshVI ATCGAT 1 cut(s) 1824
BsiHKAI GWGCWC 5 cut(s) 437, 523, 960, 2103, 2119
BsiHKCI CYCGRG 1 cut(s) 662
BsiSI CCGG 8 cut(s) 455, 683, 810, 828, 1564, 1658, 1689, 2072
BslFI GGGAC 2 cut(s) 18, 1739
BslI CCNNNNNNNGG 6 cut(s) 249, 444, 1441, 1508, 1922, 2078
BsmAI GTCTC 3 cut(s) 417, 633, 1562
BsmBI CGTCTC 1 cut(s) 1562
BsmFI GGGAC 2 cut(s) 18, 1739
BsmI GAATGC 2 cut(s) 610, 753
BsnI GGCC 4 cut(s) 120, 1351, 1745, 2002
BsoBI CYCGRG 1 cut(s) 662
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 6 cut(s) 437, 454, 523, 960, 2103, 2119
Bsp13I TCCGGA 2 cut(s) 809, 827
Bsp143I GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
Bsp1720I GCTNAGC 1 cut(s) 933
BspACI CCGC 4 cut(s) 353, 632, 1106, 2059
BspANI GGCC 4 cut(s) 120, 1351, 1745, 2002
BspCNI CTCAG 8 cut(s) 785, 925, 930, 1075, 1495, 1795, 1822, 2089
BspDI ATCGAT 1 cut(s) 1824
BspEI TCCGGA 2 cut(s) 809, 827
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 5 cut(s) 183, 453, 832, 1495, 1662
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 2 cut(s) 160, 302
BspPI GGATC 3 cut(s) 1560, 1820, 2082
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 451
BsrDI GCAATG 4 cut(s) 54, 131, 304, 1254
BsrI ACTGG 4 cut(s) 520, 1352, 1633, 2003
BssECI CCNNGG 4 cut(s) 201, 683, 755, 1589
BssMI GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
BssSI CACGAG 1 cut(s) 1380
BssT1I CCWWGG 2 cut(s) 755, 1589
Bst2BI CACGAG 1 cut(s) 1380
Bst2UI CCWGG 4 cut(s) 117, 203, 1517, 1747
Bst4CI ACNGT 7 cut(s) 439, 469, 627, 1425, 1676, 1709, 2043
Bst6I CTCTTC 3 cut(s) 1062, 1565, 1938
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 6 cut(s) 18, 644, 991, 1324, 1498, 1850
BstF5I GGATG 5 cut(s) 20, 256, 760, 925, 1468
BstFNI CGCG 1 cut(s) 353
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 7 cut(s) 495, 509, 1261, 1555, 1681, 1828, 2077
BstMAI GTCTC 3 cut(s) 417, 633, 1562
BstMBI GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
BstMWI GCNNNNNNNGC 9 cut(s) 518, 711, 757, 943, 1079, 1154, 1187, 1196, 2098
BstNI CCWGG 4 cut(s) 117, 203, 1517, 1747
BstNSI RCATGY 2 cut(s) 979, 1852
BstSCI CCNGG 6 cut(s) 115, 201, 682, 1515, 1687, 1745
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 2 cut(s) 454, 2119
BstUI CGCG 1 cut(s) 353
BstX2I RGATCY 2 cut(s) 492, 2074
BstYI RGATCY 2 cut(s) 492, 2074
Bsu15I ATCGAT 1 cut(s) 1824
BsuRI GGCC 4 cut(s) 120, 1351, 1745, 2002
BsuTUI ATCGAT 1 cut(s) 1824
BtgZI GCGATG 1 cut(s) 1582
BtsCI GGATG 5 cut(s) 20, 256, 760, 925, 1468
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 4 cut(s) 465, 641, 1626, 2048
BveI ACCTGC 2 cut(s) 160, 302
Cac8I GCNNGC 6 cut(s) 18, 644, 991, 1324, 1498, 1850
CaiI CAGNNNCTG 1 cut(s) 1769
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 1349
ClaI ATCGAT 1 cut(s) 1824
CseI GACGC 1 cut(s) 1391
Csp6I GTAC 5 cut(s) 377, 386, 1267, 1528, 1670
CviQI GTAC 5 cut(s) 377, 386, 1267, 1528, 1670
DpnI GATC 7 cut(s) 494, 508, 1260, 1554, 1680, 1827, 2076
DpnII GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
DraIII CACNNNGTG 1 cut(s) 1883
EaeI YGGCCR 1 cut(s) 1743
Eam1104I CTCTTC 3 cut(s) 1062, 1565, 1938
EarI CTCTTC 3 cut(s) 1062, 1565, 1938
EciI GGCGGA 1 cut(s) 2074
Ecl136II GAGCTC 2 cut(s) 958, 2101
Eco130I CCWWGG 2 cut(s) 755, 1589
Eco24I GRGCYC 2 cut(s) 960, 2103
Eco32I GATATC 1 cut(s) 1621
Eco53kI GAGCTC 2 cut(s) 958, 2101
Eco57I CTGAAG 2 cut(s) 213, 932
Eco88I CYCGRG 1 cut(s) 662
EcoICRI GAGCTC 2 cut(s) 958, 2101
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 4 cut(s) 115, 201, 1515, 1745
EcoRV GATATC 1 cut(s) 1621
EcoT14I CCWWGG 2 cut(s) 755, 1589
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 960, 2103
ErhI CCWWGG 2 cut(s) 755, 1589
Esp3I CGTCTC 1 cut(s) 1562
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 689, 721
FaqI GGGAC 2 cut(s) 18, 1739
FauNDI CATATG 1 cut(s) 1255
FblI GTMKAC 2 cut(s) 1521, 1872
FokI GGATG 5 cut(s) 7, 243, 747, 932, 1455
FriOI GRGCYC 2 cut(s) 960, 2103
FspBI CTAG 9 cut(s) 186, 311, 420, 756, 768, 1103, 1667, 1703, 2033
GlaI GCGC 1 cut(s) 176
HaeIII GGCC 4 cut(s) 120, 1351, 1745, 2002
HapII CCGG 8 cut(s) 455, 683, 810, 828, 1564, 1658, 1689, 2072
HgaI GACGC 1 cut(s) 1391
HhaI GCGC 1 cut(s) 177
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HindIII AAGCTT 2 cut(s) 703, 1320
HinfI GANTC 6 cut(s) 317, 411, 665, 776, 1772, 1930
HpaII CCGG 8 cut(s) 455, 683, 810, 828, 1564, 1658, 1689, 2072
HphI GGTGA 3 cut(s) 47, 569, 1376
Hpy166II GTNNAC 8 cut(s) 386, 560, 857, 1522, 1672, 1873, 2039, 2117
Hpy188III TCNNGA 7 cut(s) 24, 810, 828, 1406, 1435, 1829, 1994
Hpy8I GTNNAC 8 cut(s) 386, 560, 857, 1522, 1672, 1873, 2039, 2117
HpyAV CCTTC 6 cut(s) 6, 888, 1228, 1331, 1496, 2077
HpyCH4III ACNGT 7 cut(s) 439, 469, 627, 1425, 1676, 1709, 2043
HpyF10VI GCNNNNNNNGC 9 cut(s) 518, 711, 757, 943, 1079, 1154, 1187, 1196, 2098
HspAI GCGC 1 cut(s) 175
Kpn2I TCCGGA 2 cut(s) 809, 827
Kzo9I GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
LmnI GCTCC 4 cut(s) 163, 687, 1288, 1493
LweI GCATC 8 cut(s) 28, 29, 223, 285, 583, 769, 1399, 1477
MaeI CTAG 9 cut(s) 186, 311, 420, 756, 768, 1103, 1667, 1703, 2033
MaeIII GTNAC 7 cut(s) 196, 692, 1364, 1571, 1709, 1777, 1855
MalI GATC 7 cut(s) 494, 508, 1260, 1554, 1680, 1827, 2076
MboI GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
MboII GAAGA 8 cut(s) 326, 867, 1079, 1243, 1552, 1634, 1925, 2021
MfeI CAATTG 1 cut(s) 1281
MflI RGATCY 2 cut(s) 492, 2074
MhlI GDGCHC 6 cut(s) 437, 454, 523, 960, 2103, 2119
MlsI TGGCCA 1 cut(s) 1745
MluCI AATT 9 cut(s) 358, 592, 836, 866, 1043, 1061, 1281, 1329, 1608
MluNI TGGCCA 1 cut(s) 1745
MlyI GAGTC 3 cut(s) 674, 785, 1924
MmeI TCCRAC 1 cut(s) 788
Mox20I TGGCCA 1 cut(s) 1745
Mph1103I ATGCAT 1 cut(s) 267
MroI TCCGGA 2 cut(s) 809, 827
MscI TGGCCA 1 cut(s) 1745
MseI TTAA 5 cut(s) 99, 144, 342, 1032, 2018
MslI CAYNNNNRTG 1 cut(s) 553
Msp20I TGGCCA 1 cut(s) 1745
MspI CCGG 8 cut(s) 455, 683, 810, 828, 1564, 1658, 1689, 2072
MspR9I CCNGG 6 cut(s) 117, 203, 684, 1517, 1689, 1747
MunI CAATTG 1 cut(s) 1281
Mva1269I GAATGC 2 cut(s) 610, 753
MvaI CCWGG 4 cut(s) 117, 203, 1517, 1747
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 9 cut(s) 518, 711, 757, 943, 1079, 1154, 1187, 1196, 2098
NciI CCSGG 2 cut(s) 684, 1689
NdeI CATATG 1 cut(s) 1255
NdeII GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
NlaIV GGNNCC 5 cut(s) 183, 453, 832, 1495, 1662
NmuCI GTSAC 3 cut(s) 196, 1364, 1777
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 2 cut(s) 979, 1852
NspV TTCGAA 1 cut(s) 590
PaeI GCATGC 1 cut(s) 1852
PaeR7I CTCGAG 1 cut(s) 662
PciI ACATGT 1 cut(s) 975
PctI GAATGC 2 cut(s) 610, 753
PfeI GAWTC 3 cut(s) 317, 411, 1772
PflFI GACNNNGTC 1 cut(s) 625
PflMI CCANNNNNTGG 1 cut(s) 249
PleI GAGTC 3 cut(s) 673, 784, 1924
PpsI GAGTC 3 cut(s) 673, 784, 1924
PscI ACATGT 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 960, 2103
Psp6I CCWGG 4 cut(s) 115, 201, 1515, 1745
PspGI CCWGG 4 cut(s) 115, 201, 1515, 1745
PspN4I GGNNCC 5 cut(s) 183, 453, 832, 1495, 1662
PspPI GGNCC 1 cut(s) 1349
PspXI VCTCGAGB 1 cut(s) 662
PsrI GAACNNNNNNTAC 2 cut(s) 1653, 1685
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 1769
PsuI RGATCY 2 cut(s) 492, 2074
PsyI GACNNNGTC 1 cut(s) 625
RsaI GTAC 5 cut(s) 378, 387, 1268, 1529, 1671
RsaNI GTAC 5 cut(s) 377, 386, 1267, 1528, 1670
RseI CAYNNNNRTG 1 cut(s) 553
SacI GAGCTC 2 cut(s) 960, 2103
SaqAI TTAA 5 cut(s) 99, 144, 342, 1032, 2018
Sau3AI GATC 7 cut(s) 492, 506, 1258, 1552, 1678, 1825, 2074
Sau96I GGNCC 1 cut(s) 1349
SchI GAGTC 3 cut(s) 674, 785, 1924
ScrFI CCNGG 6 cut(s) 117, 203, 684, 1517, 1689, 1747
SduI GDGCHC 6 cut(s) 437, 454, 523, 960, 2103, 2119
SfaNI GCATC 8 cut(s) 28, 29, 223, 285, 583, 769, 1399, 1477
SfcI CTRYAG 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 662
SfuI TTCGAA 1 cut(s) 590
SlaI CTCGAG 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 553
SmlI CTYRAG 4 cut(s) 237, 662, 959, 1416
SmoI CTYRAG 4 cut(s) 237, 662, 959, 1416
SphI GCATGC 1 cut(s) 1852
Sse9I AATT 9 cut(s) 358, 592, 836, 866, 1043, 1061, 1281, 1329, 1608
SsiI CCGC 4 cut(s) 353, 632, 1106, 2059
SspI AATATT 1 cut(s) 1452
SspMI CTAG 9 cut(s) 186, 311, 420, 756, 768, 1103, 1667, 1703, 2033
SstI GAGCTC 2 cut(s) 960, 2103
StyD4I CCNGG 6 cut(s) 115, 201, 682, 1515, 1687, 1745
StyI CCWWGG 2 cut(s) 755, 1589
TaaI ACNGT 7 cut(s) 439, 469, 627, 1425, 1676, 1709, 2043
TaqI TCGA 5 cut(s) 366, 590, 663, 797, 1824
TaqII GACCGA 1 cut(s) 1765
TasI AATT 9 cut(s) 358, 592, 836, 866, 1043, 1061, 1281, 1329, 1608
TatI WGTACW 2 cut(s) 1527, 1669
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 3 cut(s) 317, 411, 1772
Tru1I TTAA 5 cut(s) 99, 144, 342, 1032, 2018
Tru9I TTAA 5 cut(s) 99, 144, 342, 1032, 2018
TscAI CASTG 4 cut(s) 472, 641, 1633, 2048
TseFI GTSAC 3 cut(s) 196, 1364, 1777
Tsp45I GTSAC 3 cut(s) 196, 1364, 1777
TspDTI ATGAA 7 cut(s) 128, 245, 315, 617, 861, 879, 1634
TspRI CASTG 4 cut(s) 472, 641, 1633, 2048
Tth111I GACNNNGTC 1 cut(s) 625
Van91I CCANNNNNTGG 1 cut(s) 249
VneI GTGCAC 1 cut(s) 2115
XapI RAATTY 4 cut(s) 358, 592, 836, 866
XceI RCATGY 2 cut(s) 979, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 1918
XhoI CTCGAG 1 cut(s) 662
XmaJI CCTAGG 1 cut(s) 755
XmiI GTMKAC 2 cut(s) 1521, 1872
XspI CTAG 9 cut(s) 186, 311, 420, 756, 768, 1103, 1667, 1703, 2033
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.