Rw2G029220

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000353
Physical Location & Seq
Forward (+)
47264475 .. 47271984
7510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029220.1

Sequence Viewer

Length: 2265 bp
ATGAGAGGCAGAGGGCACGACGCAGAACAACAATTAAAAGAACAGGCAGTGGCAGTGGCCATAGACAAGGACAAAGGAAGTGAATATGCAATCAAATGGGCTATTGATAGTCTCTTAACCAAGGGCCAACCTCTCACATTGCTCCATGTTAGACATCCATATCCTGGCCGGACTGCGTCAACGGAGAATCAAAAAGATCCCCAATCACATGAGTTATTTCTTTCGTTTCGTTCCTTTTGCGCAAAAAAAAATATAAAATGCACTGAAGTCATACTCGAGGACAATGACATACCAAACGCACTGATCAAGTATGTTAATGCAAATTCAATTGAGATTTTGGTACTTGGTGTTCCAACAAGAGGTGGCATTTTCAGATTCAAATTTACAGATGTACCGAGCAGTGTGATAAAAATGACGCCAGAATTTTGCTCTGTTTATGTCATCGCTAAAGGAAAAATCTCATCTATGCGATGCTCCACCGTAGCACCGCCACAGCCTCCTCCACGTAGTAATCATCAAATGCAACACCATCCCAGCAATGTTTCTGAAGCAAGTGATTCGCATTTCATCCGCACCCAGAGCCTAACAGCAGAGGTGTGGTCACCATTCACTAGAGGAAGACCTTCGATGGACAGATCATTTGAGCTCCCACCAGATAGTGACATATCGTTTGTGAGTAGTGGAAGACCAAGTGTGGATCGCATGTATCCTTCTTTCTATGATAGTATGGATTCAGGGAGGAACTCTCGACGTTTGATTGGCTCAGAAACGGAAAACAGAAGCTCTGTGTCATCCAACCCGAGAAGCAAGCATTTTATGGAAATCAACTCTCCACAACTTGAATTTTCACCATCCTCAGTTGATAGTGGAAACTCTTGGTTATCACAAAATAATAATAATAATTTGGAAGATGTGGAGCTTGAGATGAGGAGGCTCAGGCTAGAGCTCAAGCAAACAATGGAGATGTACAATACAGCGTGCAAAGAAGCACTGACAGCAAAACATAAGGAAAATGAATTTAACCGGTGCAAAATGGAAGAAGGACAAAGATTAGAACAGGCGCGACTAGCTGAGGAAGCTGCATTGGCACTTGTAGAGAGGGAGAAAGCAAAATGCAAGTCAGCGATTGAAGCAGCTGCAGCAGCTCAAAGGCTTGCTGAACTGGAAGCGCAAAAGAGGAGGAACGCAGAAATGAAAGCCCTTAAAGAATCCGAAGAGAGGAAGAAGACACTTGAAGCAAAGTCATGTGATGTTAGGTACAGGAAGTACACAATTGAGGAGATTGAAGTGGCAACAGATAAATTTTCACTAGCTCGCAAGGTTGGGGAAGGAGGCTATGGGCCAGTATACAGAGGTGAACTGCATCATACACCAGTTGCTATAAAAGTTCTACGTCCGGATGCAGCTCAAGGGCGATCGCAGTTCCAGCTAGAGGTTGAAGTATTGAGCTGCATACGACATCCAAACATGGTTCTCCTTCTTGGAGCTTGTCCAGAGTACGGTTGTTTAGTCTATGAGTACATGGCTAATGGGAGCTTGGAAAATCGTCTATTTCACCGAGGTAACACCCCGGCTATTCCTTGGCAACTAAGGTTTCGAATTGCTGCAGAAATTGCAACGGGGCTCTTATTCCTTCATCAGGCTAAGCCAGAACCCCTTGTGCACCGTGACTTAAAACCTGGCAACATATTGCTAGATAATAACTATGTGAGCAAGATTAGTGATGTAGGTTTGGCTAGACTTGTGCCTCCATCAATAGCTGATGCGGTCACACAGTATCATATGACATCAACAGCTGGAACATTTTGCTACATTGATCCAGAGTATCAGCAAACAGGCATGCTTGGAACAAAATCTGATATCTACTCACTAGGTGTGATGCTTCTACAAATCATTACAGCCAAACCACCAATGGGTTTGACTCATCATGTAGAGCGGGCTATTGAGACGGGAAGCTTTAGTGAGATGCTTGATCCTGCAGTTCCTGATTGGCCAGTTGAGGAAGCCTTGAAGTTTGCCAAACTTTCTTTACAGTGTGCTGAAATGAGAAAAAAAGACCGACCCGATCTTGGGAAGGTTATACTGCCTGAGCTTAACAGATTGAGAGCCCTCGCCGACGATAGCTTCCAATGTGTTATGTTTGGCGGAAGTGGAGTACTCTCACCAAATCAAGACTCAAATTCTACATCACAAGTGAAGCCACCATATATACGATATATGTTAAGCAGCTATGACACGGCCACATCATTTATTAAAAGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

754

Amino Acids

84.53

Weight (kDa)

7.85

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 17 - 140 3.4e-11 Universal stress protein family
Pkinase PF00069 436 - 688 9.4e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 437 - 693 6.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 241
AccB7I CCANNNNNTGG 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 1936
AccI GTMKAC 1 cut(s) 1347
AccII CGCG 1 cut(s) 1063
AccIII TCCGGA 1 cut(s) 1396
AciI CCGC 5 cut(s) 488, 571, 1766, 1936, 2145
AclWI GGATC 4 cut(s) 191, 705, 1811, 1967
AcoI YGGCCR 4 cut(s) 57, 166, 1991, 2238
AcsI RAATTY 7 cut(s) 322, 380, 422, 842, 1016, 1301, 2179
AcuI CTGAAG 2 cut(s) 285, 567
AcyI GRCGYC 1 cut(s) 416
AdeI CACNNNGTG 1 cut(s) 1874
AfaI GTAC 8 cut(s) 342, 393, 968, 1259, 1268, 1499, 1520, 2157
AfiI CCNNNNNNNGG 9 cut(s) 164, 359, 1218, 1432, 1499, 1639, 1913, 2069, 2070
AgeI ACCGGT 1 cut(s) 1023
AgsI TTSAA 8 cut(s) 327, 379, 842, 1130, 1235, 1286, 1439, 2011
AjnI CCWGG 2 cut(s) 163, 1678
Alw21I GWGCWC 3 cut(s) 648, 948, 1665
Alw26I GTCTC 2 cut(s) 116, 1940
Alw44I GTGCAC 1 cut(s) 1661
AlwI GGATC 4 cut(s) 191, 705, 1811, 1967
AlwNI CAGNNNCTG 1 cut(s) 1985
Ama87I CYCGRG 2 cut(s) 275, 799
Aor13HI TCCGGA 1 cut(s) 1396
AoxI GGCC 6 cut(s) 57, 124, 166, 1340, 1991, 2238
ApaLI GTGCAC 1 cut(s) 1661
ApeKI GCWGC 9 cut(s) 1079, 1133, 1136, 1139, 1142, 1403, 1449, 1604, 2226
ApoI RAATTY 7 cut(s) 322, 380, 422, 842, 1016, 1301, 2179
ArsI GACNNNNNNTTYG 6 cut(s) 623, 653, 655, 685, 1103, 1135
AsiGI ACCGGT 1 cut(s) 1023
AsiSI GCGATCGC 1 cut(s) 1418
Asp700I GAANNNNTTC 1 cut(s) 622
AspLEI GCGC 3 cut(s) 242, 1063, 1171
AspS9I GGNCC 2 cut(s) 124, 1340
AsuC2I CCSGG 1 cut(s) 1571
AsuHPI GGTGA 5 cut(s) 594, 840, 1367, 1547, 2154
AsuII TTCGAA 1 cut(s) 1597
AvaI CYCGRG 2 cut(s) 275, 799
BaeGI GKGCMC 2 cut(s) 18, 1665
BaeI ACNNNNGTAYC 2 cut(s) 375, 408
BalI TGGCCA 2 cut(s) 59, 1993
BanII GRGCYC 4 cut(s) 648, 948, 1626, 2110
BbsI GAAGAC 3 cut(s) 625, 691, 1232
Bbv12I GWGCWC 3 cut(s) 648, 948, 1665
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 9 cut(s) 1066, 1123, 1145, 1151, 1154, 1415, 1436, 1591, 2238
BccI CCATC 4 cut(s) 537, 622, 859, 1759
BceAI ACGGC 1 cut(s) 2253
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 2 cut(s) 165, 1680
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 1 cut(s) 1571
BcoDI GTCTC 2 cut(s) 116, 1940
BfaI CTAG 8 cut(s) 612, 941, 1067, 1310, 1430, 1694, 1737, 1871
BfmI CTRYAG 3 cut(s) 1137, 1605, 1977
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 9 cut(s) 1080, 1134, 1137, 1140, 1143, 1404, 1450, 1605, 2227
BlpI GCTNAGC 1 cut(s) 1644
BlsI GCNGC 9 cut(s) 1081, 1135, 1138, 1141, 1144, 1405, 1451, 1606, 2228
BmcAI AGTACT 1 cut(s) 2157
Bme1390I CCNGG 3 cut(s) 165, 1571, 1680
BmeT110I CYCGRG 2 cut(s) 275, 799
BmgT120I GGNCC 2 cut(s) 124, 1340
BmrFI CCNGG 3 cut(s) 165, 1571, 1680
BmsI GCATC 6 cut(s) 461, 1372, 1390, 1753, 1869, 1956
BpiI GAAGAC 3 cut(s) 625, 691, 1232
BplI GAGNNNNNCTC 2 cut(s) 730, 762
Bpu10I CCTNAGC 3 cut(s) 935, 1071, 2088
Bpu1102I GCTNAGC 1 cut(s) 1644
Bpu14I TTCGAA 1 cut(s) 1597
BpuEI CTTGAG 3 cut(s) 932, 941, 1392
BpuMI CCSGG 1 cut(s) 1571
BsaAI YACGTR 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 4 cut(s) 120, 1558, 1569, 1580
BsaWI WCCGGW 2 cut(s) 1023, 1396
Bsc4I CCNNNNNNNGG 9 cut(s) 164, 359, 1218, 1432, 1499, 1639, 1913, 2069, 2070
Bse118I RCCGGY 1 cut(s) 1023
Bse1I ACTGG 4 cut(s) 1167, 1343, 1373, 1994
Bse3DI GCAATG 2 cut(s) 137, 544
BseAI TCCGGA 1 cut(s) 1396
BseBI CCWGG 2 cut(s) 165, 1680
BseDI CCNNGG 4 cut(s) 120, 1558, 1569, 1580
BseGI GGATG 7 cut(s) 154, 529, 567, 791, 851, 1405, 1459
BseLI CCNNNNNNNGG 9 cut(s) 164, 359, 1218, 1432, 1499, 1639, 1913, 2069, 2070
BseMI GCAATG 2 cut(s) 137, 544
BseMII CTCAG 5 cut(s) 777, 870, 949, 1062, 2079
BseNI ACTGG 4 cut(s) 1167, 1343, 1373, 1994
BseRI GAGGAG 4 cut(s) 489, 943, 1192, 1292
BseSI GKGCMC 2 cut(s) 18, 1665
BseXI GCAGC 9 cut(s) 1066, 1123, 1145, 1151, 1154, 1415, 1436, 1591, 2238
BseYI CCCAGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 1063
Bsh1285I CGRYCG 1 cut(s) 1418
BshFI GGCC 6 cut(s) 59, 126, 168, 1342, 1993, 2240
BshTI ACCGGT 1 cut(s) 1023
BsiEI CGRYCG 1 cut(s) 1418
BsiHKAI GWGCWC 3 cut(s) 648, 948, 1665
BsiHKCI CYCGRG 2 cut(s) 275, 799
BsiSI CCGG 4 cut(s) 169, 1024, 1397, 1571
BslI CCNNNNNNNGG 9 cut(s) 164, 359, 1218, 1432, 1499, 1639, 1913, 2069, 2070
BsmAI GTCTC 2 cut(s) 116, 1940
BsmBI CGTCTC 1 cut(s) 1940
BsnI GGCC 6 cut(s) 59, 126, 168, 1342, 1993, 2240
BsoBI CYCGRG 2 cut(s) 275, 799
Bsp119I TTCGAA 1 cut(s) 1597
Bsp1286I GDGCHC 6 cut(s) 18, 648, 948, 1626, 1665, 2110
Bsp13I TCCGGA 1 cut(s) 1396
Bsp1407I TGTACA 1 cut(s) 966
Bsp143I GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
Bsp1720I GCTNAGC 1 cut(s) 1644
BspACI CCGC 5 cut(s) 488, 571, 1766, 1936, 2145
BspANI GGCC 6 cut(s) 59, 126, 168, 1342, 1993, 2240
BspCNI CTCAG 5 cut(s) 776, 869, 948, 1063, 2080
BspEI TCCGGA 1 cut(s) 1396
BspFNI CGCG 1 cut(s) 1063
BspMAI CTGCAG 3 cut(s) 1141, 1609, 1981
BspPI GGATC 4 cut(s) 191, 705, 1811, 1967
BspT104I TTCGAA 1 cut(s) 1597
BsrBI CCGCTC 1 cut(s) 1936
BsrDI GCAATG 2 cut(s) 137, 544
BsrFI RCCGGY 1 cut(s) 1023
BsrGI TGTACA 1 cut(s) 966
BsrI ACTGG 4 cut(s) 1167, 1343, 1373, 1994
BssAI RCCGGY 1 cut(s) 1023
BssECI CCNNGG 4 cut(s) 120, 1558, 1569, 1580
BssMI GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
BssNAI GTATAC 1 cut(s) 1348
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 120, 1580
Bst1107I GTATAC 1 cut(s) 1348
Bst2UI CCWGG 2 cut(s) 165, 1680
Bst4CI ACNGT 5 cut(s) 481, 1502, 1667, 1776, 2034
Bst6I CTCTTC 1 cut(s) 1209
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 1613
BstAUI TGTACA 1 cut(s) 966
BstBAI YACGTR 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 1597
BstC8I GCNNGC 6 cut(s) 809, 979, 1155, 1315, 1841, 1938
BstDEI CTNAG 7 cut(s) 763, 856, 935, 1071, 1589, 1644, 2088
BstEII GGTNACC 1 cut(s) 600
BstENI CCTNNNNNAGG 1 cut(s) 1637
BstF5I GGATG 7 cut(s) 154, 529, 567, 791, 851, 1405, 1459
BstFNI CGCG 1 cut(s) 1063
BstHHI GCGC 3 cut(s) 242, 1063, 1171
BstKTI GATC 8 cut(s) 199, 306, 638, 700, 1418, 1819, 1975, 2068
BstMAI GTCTC 2 cut(s) 116, 1940
BstMBI GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
BstMCI CGRYCG 1 cut(s) 1418
BstNI CCWGG 2 cut(s) 165, 1680
BstNSI RCATGY 2 cut(s) 706, 1843
BstPI GGTNACC 1 cut(s) 600
BstSCI CCNGG 3 cut(s) 163, 1569, 1678
BstSFI CTRYAG 3 cut(s) 1137, 1605, 1977
BstSLI GKGCMC 2 cut(s) 18, 1665
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 9 cut(s) 1066, 1123, 1145, 1151, 1154, 1415, 1436, 1591, 2238
BstV2I GAAGAC 3 cut(s) 625, 691, 1232
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BstZ17I GTATAC 1 cut(s) 1348
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 6 cut(s) 59, 126, 168, 1342, 1993, 2240
BtgZI GCGATG 2 cut(s) 427, 484
BtsCI GGATG 7 cut(s) 154, 529, 567, 791, 851, 1405, 1459
BtsI GCAGTG 3 cut(s) 54, 60, 406
BtsIMutI CAGTG 7 cut(s) 54, 60, 261, 299, 406, 989, 2039
Cac8I GCNNGC 6 cut(s) 809, 979, 1155, 1315, 1841, 1938
CaiI CAGNNNCTG 1 cut(s) 1985
CfoI GCGC 3 cut(s) 242, 1063, 1171
Cfr10I RCCGGY 1 cut(s) 1023
Cfr13I GGNCC 2 cut(s) 124, 1340
CseI GACGC 3 cut(s) 29, 165, 424
Csp6I GTAC 8 cut(s) 341, 392, 967, 1258, 1267, 1498, 1519, 2156
CspAI ACCGGT 1 cut(s) 1023
CviAII CATG 8 cut(s) 146, 209, 703, 1245, 1468, 1522, 1840, 1928
CviQI GTAC 8 cut(s) 341, 392, 967, 1258, 1267, 1498, 1519, 2156
DdeI CTNAG 7 cut(s) 763, 856, 935, 1071, 1589, 1644, 2088
DpnI GATC 8 cut(s) 198, 305, 637, 699, 1417, 1818, 1974, 2067
DpnII GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
DraIII CACNNNGTG 1 cut(s) 1874
EaeI YGGCCR 4 cut(s) 57, 166, 1991, 2238
Eam1104I CTCTTC 1 cut(s) 1209
EarI CTCTTC 1 cut(s) 1209
EciI GGCGGA 1 cut(s) 2160
Ecl136II GAGCTC 2 cut(s) 646, 946
Eco130I CCWWGG 2 cut(s) 120, 1580
Eco24I GRGCYC 4 cut(s) 648, 948, 1626, 2110
Eco32I GATATC 1 cut(s) 1861
Eco53kI GAGCTC 2 cut(s) 646, 946
Eco57I CTGAAG 2 cut(s) 285, 567
Eco88I CYCGRG 2 cut(s) 275, 799
Eco91I GGTNACC 1 cut(s) 600
EcoICRI GAGCTC 2 cut(s) 646, 946
EcoNI CCTNNNNNAGG 1 cut(s) 1637
EcoO65I GGTNACC 1 cut(s) 600
EcoRII CCWGG 2 cut(s) 163, 1678
EcoRV GATATC 1 cut(s) 1861
EcoT14I CCWWGG 2 cut(s) 120, 1580
EcoT38I GRGCYC 4 cut(s) 648, 948, 1626, 2110
ErhI CCWWGG 2 cut(s) 120, 1580
Esp3I CGTCTC 1 cut(s) 1940
FaeI CATG 8 cut(s) 149, 212, 706, 1248, 1471, 1525, 1843, 1931
FatI CATG 8 cut(s) 145, 208, 702, 1244, 1467, 1521, 1839, 1927
FauI CCCGC 1 cut(s) 1929
FauNDI CATATG 1 cut(s) 1782
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 1347
Fnu4HI GCNGC 9 cut(s) 1080, 1134, 1137, 1140, 1143, 1404, 1450, 1605, 2227
FokI GGATG 7 cut(s) 141, 516, 554, 778, 838, 1412, 1446
FriOI GRGCYC 4 cut(s) 648, 948, 1626, 2110
Fsp4HI GCNGC 9 cut(s) 1080, 1134, 1137, 1140, 1143, 1404, 1450, 1605, 2227
FspBI CTAG 8 cut(s) 612, 941, 1067, 1310, 1430, 1694, 1737, 1871
FspI TGCGCA 1 cut(s) 241
GlaI GCGC 3 cut(s) 241, 1062, 1170
GluI GCNGC 9 cut(s) 1080, 1134, 1137, 1140, 1143, 1404, 1450, 1605, 2227
GsaI CCCAGC 1 cut(s) 537
HaeIII GGCC 6 cut(s) 59, 126, 168, 1342, 1993, 2240
HapII CCGG 4 cut(s) 169, 1024, 1397, 1571
HgaI GACGC 3 cut(s) 29, 165, 424
HhaI GCGC 3 cut(s) 242, 1063, 1171
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 8 cut(s) 149, 212, 706, 1248, 1471, 1525, 1843, 1931
Hin6I GCGC 3 cut(s) 240, 1061, 1169
HinP1I GCGC 3 cut(s) 240, 1061, 1169
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 1954
HinfI GANTC 7 cut(s) 187, 375, 557, 731, 1208, 1921, 2174
HpaII CCGG 4 cut(s) 169, 1024, 1397, 1571
HphI GGTGA 5 cut(s) 594, 840, 1367, 1547, 2154
Hpy166II GTNNAC 5 cut(s) 180, 1269, 1348, 1358, 1663
Hpy188I TCNGA 5 cut(s) 374, 547, 766, 1213, 1858
Hpy188III TCNNGA 6 cut(s) 747, 1397, 1493, 1820, 1985, 2171
Hpy8I GTNNAC 5 cut(s) 180, 1269, 1348, 1358, 1663
Hpy99I CGWCG 3 cut(s) 23, 753, 2120
HpyAV CCTTC 7 cut(s) 633, 720, 1034, 1322, 1487, 1643, 2068
HpyCH4III ACNGT 5 cut(s) 481, 1502, 1667, 1776, 2034
HpyCH4IV ACGT 3 cut(s) 505, 751, 1393
HpyF3I CTNAG 7 cut(s) 763, 856, 935, 1071, 1589, 1644, 2088
HpySE526I ACGT 3 cut(s) 505, 751, 1393
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 8 cut(s) 149, 212, 706, 1248, 1471, 1525, 1843, 1931
HspAI GCGC 3 cut(s) 240, 1061, 1169
Kpn2I TCCGGA 1 cut(s) 1396
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
LmnI GCTCC 6 cut(s) 147, 479, 651, 916, 1484, 1533
Lsp1109I GCAGC 9 cut(s) 1066, 1123, 1145, 1151, 1154, 1415, 1436, 1591, 2238
LweI GCATC 6 cut(s) 461, 1372, 1390, 1753, 1869, 1956
MaeI CTAG 8 cut(s) 612, 941, 1067, 1310, 1430, 1694, 1737, 1871
MaeII ACGT 3 cut(s) 505, 751, 1393
MaeIII GTNAC 5 cut(s) 600, 659, 1562, 1667, 1768
MalI GATC 8 cut(s) 198, 305, 637, 699, 1417, 1818, 1974, 2067
MbiI CCGCTC 1 cut(s) 1936
MboI GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
MboII GAAGA 7 cut(s) 630, 696, 920, 1049, 1226, 1234, 1237
MfeI CAATTG 2 cut(s) 327, 1272
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 6 cut(s) 18, 648, 948, 1626, 1665, 2110
MlsI TGGCCA 2 cut(s) 59, 1993
MluNI TGGCCA 2 cut(s) 59, 1993
MlyI GAGTC 2 cut(s) 1915, 2168
MmeI TCCRAC 2 cut(s) 377, 819
Mox20I TGGCCA 2 cut(s) 59, 1993
MroI TCCGGA 1 cut(s) 1396
MroXI GAANNNNTTC 1 cut(s) 622
MscI TGGCCA 2 cut(s) 59, 1993
MseI TTAA 9 cut(s) 35, 116, 315, 1020, 1203, 1673, 2094, 2222, 2253
Msp20I TGGCCA 2 cut(s) 59, 1993
MspA1I CMGCKG 2 cut(s) 1136, 1796
MspI CCGG 4 cut(s) 169, 1024, 1397, 1571
MspR9I CCNGG 3 cut(s) 165, 1571, 1680
MunI CAATTG 2 cut(s) 327, 1272
MvaI CCWGG 2 cut(s) 165, 1680
MvnI CGCG 1 cut(s) 1063
NciI CCSGG 1 cut(s) 1571
NdeI CATATG 1 cut(s) 1782
NdeII GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
NlaIII CATG 8 cut(s) 149, 212, 706, 1248, 1471, 1525, 1843, 1931
NmuCI GTSAC 4 cut(s) 600, 659, 1667, 1768
NsbI TGCGCA 1 cut(s) 241
NspI RCATGY 2 cut(s) 706, 1843
NspV TTCGAA 1 cut(s) 1597
PaeI GCATGC 1 cut(s) 1843
PaeR7I CTCGAG 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 187, 375, 557, 731, 1208
PflFI GACNNNGTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 1023
PkrI GCNGC 9 cut(s) 1081, 1135, 1138, 1141, 1144, 1405, 1451, 1606, 2228
Ple19I CGATCG 1 cut(s) 1418
PleI GAGTC 2 cut(s) 1915, 2168
PpsI GAGTC 2 cut(s) 1915, 2168
Ppu21I YACGTR 1 cut(s) 506
Psp124BI GAGCTC 2 cut(s) 648, 948
Psp6I CCWGG 2 cut(s) 163, 1678
PspEI GGTNACC 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 2 cut(s) 163, 1678
PspPI GGNCC 2 cut(s) 124, 1340
PspXI VCTCGAGB 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 333, 365
PstI CTGCAG 3 cut(s) 1141, 1609, 1981
PstNI CAGNNNCTG 1 cut(s) 1985
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 175
PvuI CGATCG 1 cut(s) 1418
PvuII CAGCTG 2 cut(s) 1136, 1796
RgaI GCGATCGC 1 cut(s) 1418
RsaI GTAC 8 cut(s) 342, 393, 968, 1259, 1268, 1499, 1520, 2157
RsaNI GTAC 8 cut(s) 341, 392, 967, 1258, 1267, 1498, 1519, 2156
SacI GAGCTC 2 cut(s) 648, 948
SaqAI TTAA 9 cut(s) 35, 116, 315, 1020, 1203, 1673, 2094, 2222, 2253
SatI GCNGC 9 cut(s) 1080, 1134, 1137, 1140, 1143, 1404, 1450, 1605, 2227
Sau3AI GATC 8 cut(s) 196, 303, 635, 697, 1415, 1816, 1972, 2065
Sau96I GGNCC 2 cut(s) 124, 1340
ScaI AGTACT 1 cut(s) 2157
SchI GAGTC 2 cut(s) 1915, 2168
ScrFI CCNGG 3 cut(s) 165, 1571, 1680
SduI GDGCHC 6 cut(s) 18, 648, 948, 1626, 1665, 2110
SfaAI GCGATCGC 1 cut(s) 1418
SfaNI GCATC 6 cut(s) 461, 1372, 1390, 1753, 1869, 1956
SfcI CTRYAG 3 cut(s) 1137, 1605, 1977
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 1597
SgfI GCGATCGC 1 cut(s) 1418
SlaI CTCGAG 1 cut(s) 275
SmlI CTYRAG 4 cut(s) 275, 920, 947, 1407
SmoI CTYRAG 4 cut(s) 275, 920, 947, 1407
SphI GCATGC 1 cut(s) 1843
SsiI CCGC 5 cut(s) 488, 571, 1766, 1936, 2145
SspMI CTAG 8 cut(s) 612, 941, 1067, 1310, 1430, 1694, 1737, 1871
SstI GAGCTC 2 cut(s) 648, 948
StyD4I CCNGG 3 cut(s) 163, 1569, 1678
StyI CCWWGG 2 cut(s) 120, 1580
TaaI ACNGT 5 cut(s) 481, 1502, 1667, 1776, 2034
TaiI ACGT 3 cut(s) 508, 754, 1396
TaqI TCGA 4 cut(s) 276, 626, 748, 1597
TaqII GACCGA 1 cut(s) 2073
TatI WGTACW 4 cut(s) 966, 1266, 1518, 2155
TfiI GAWTC 5 cut(s) 187, 375, 557, 731, 1208
Tru1I TTAA 9 cut(s) 35, 116, 315, 1020, 1203, 1673, 2094, 2222, 2253
Tru9I TTAA 9 cut(s) 35, 116, 315, 1020, 1203, 1673, 2094, 2222, 2253
TscAI CASTG 7 cut(s) 54, 60, 268, 306, 406, 996, 2039
TseFI GTSAC 4 cut(s) 600, 659, 1667, 1768
TseI GCWGC 9 cut(s) 1079, 1133, 1136, 1139, 1142, 1403, 1449, 1604, 2226
Tsp45I GTSAC 4 cut(s) 600, 659, 1667, 1768
TspDTI ATGAA 4 cut(s) 556, 1029, 1208, 1625
TspGWI ACGGA 2 cut(s) 197, 785
TspRI CASTG 7 cut(s) 54, 60, 268, 306, 406, 996, 2039
Tth111I GACNNNGTC 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 164
VneI GTGCAC 1 cut(s) 1661
XagI CCTNNNNNAGG 1 cut(s) 1637
XapI RAATTY 7 cut(s) 322, 380, 422, 842, 1016, 1301, 2179
XceI RCATGY 2 cut(s) 706, 1843
XcmI CCANNNNNNNNNTGG 1 cut(s) 1909
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 1347
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 8 cut(s) 612, 941, 1067, 1310, 1430, 1694, 1737, 1871
ZrmI AGTACT 1 cut(s) 2157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.