RLG00000019421

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000353
Physical Location & Seq
Reverse (-)
51857968 .. 51862372
4405 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019421

Sequence Viewer

Length: 2256 bp
ATGGGAATCAGAGGTAAAGCACAGAAGAGAGAAGAGGCAGTGGCGGTGGCCATCGACAAGGACAAAGGAAGTCAATATGCTATCAAATGGACTGTTGATCATCTTCTCAGCAGAGGCCAAGCTCTCACATTGCTTCATGTTAAACAGACTACAAATGCAGTTCCCAGCCAATCCAGAGGCACGACTTTTATGTCTGAGGATGTTGCAAAGGTGTACAAAAAACAACTTGATGTTCAAGCAAAAGAGTTGTTTCTTCCATTCCGTTGCTTTTGCACCAAAAAGGCTATAAAATGCAATGAAGTCATAGTCGAGGATACAGATATAACAAGAGGAATCATCAACTATGTCAATGCCAATGGGATTGAGATTTTGGTACTTGGTTCTCCATCAAAAAGCAGCTTGTTCAGATTCATATCTACAGACGTTCCAACCACCGTGGCGAAAAGGGCACCAGACTGTTGCACTGTTTATGTTATTGGTAAAGGAAAGATCTCATATGTGCGATCCGCTACCAGCTCATTGCCCCCAAAAGCTCTATCGAGTAATCAAATACAGCACCAAGCTACCAACGTTTCGGATTCAAGTGATACACGAAGCACACCCAGCCAGAACTTTAGAGGACCCGAGCAGAAACAGCATCCGCCCCATACCCAGCAAAATGACAATGCAATCCTGTCACCATTCTCAAGAGGAAAAGCTTCAATGAACAAAACACATGAGCTATCACTCGAGAGTGATATATCGTTTGTGAGCAGTAACGGAAGGTCAAACACTAATAACAGAAGCTCTACATCATCTTACTCTGGAACCAAGTTCATTGACATGGGCTCTGCCCACAATGACTTCTCATCATGCTCAATGGATAGTGGCAAATCGTGGTCATCACAGAACATGGATGGAGTGGAATCGGAAATGAGGAGGCTTATGCTAGAGCTCAAGCAAACAAGGGACATGTACAATACAGCCTGCAAGGAGGCACTCGTAGCACAAAATAGGGCAACAGAAGTAAACCAATGGAAAAGGGCAGAAGAAATAAGATTAGAAGAGGCAAAAGTAGCCGAGGAAGTTGCAATGGCAACTGCAGAGAAGGAGAAAGCCAAATGTATGGCAGCCATCGAAGCAGCACAAGCGGCTCAAAGGGTTGTAGAATTCGAGGCACTGAAAAGGAAGAGTGCAGAAATGAAAGCCCTTAAAGAAGAGGAAGACAGGAAGAAGGCACAAGAAGGAATGACATTTGATGTCAGGTACAGGAAGTACACACTTGAAGAGATTGAAGCAGCAACACACAGCTTTTCAGGTAGTCTCAAGATTGGGGAAGGAGGCTATGGGCCTGTGTATAAAGGTGAACTAGACCATACATCTGTGGCCATAAAAGTTCTACGTCCAGATGCAGCTCAAGGGCATTCACAGTTCAAGCGAGAGGTGGATGTGTTGAGCTGCATACGACATCCAAACATGGTCCTCCTTCTTGGAGCATGTCCCGAGTACGGTTGCCTTGTCTACGAGTACATGGCTAATGGTAGCTTAGAAGATCGTCTCTTCCGGAAAGGTAATACCCCAGCTATTCCTTGGCAGCTAAGGTTTCGAATTGCAGCAGAAATTGCCACAGGGCTTTTGTTCCTCCATCAGAGCAAGCCAGAACCCCTTGTACACCGTGACCTTAAACCTGGAAATATTTTGCTGGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTTTGGCTAGACTTGTCCCTCCATCAGTAGCCGACTCTGTCACCCAGTATTACATGACATCAACAGCAGGAACATTCTGCTATATTGATCCAGAGTATCAGCAAACTGGCATGCTTGGAACAAAATCTGACATCTATTCACTTGGAGTCATGCTTCTGCAAATCATAACAACCAAGCCACCAATGGGTTTGACACATCATGTTGAGAGGGCTATTGATAAAGGGACCTTTGCTGACATGCTTGACCCTGCTGTTAAAGACTGGCCTGTTGAGGAGGCCTTGAACTTTGCCAGGCTAGCTTTACAGTGTGCTGAAATGAGGCGAAAAGACAGACCGGATCTTGGGAAGGTTGTGCTACCTGAGCTTAACAGGTTGAGAGCGCTTGGCCAAGATAGCATAAATGCTAAGATGTTTGGTGGCGGTGAAGTATTCTCTCCAAGAAAAGGCCGATTTTCCACCGGACAAGATGTGATGAGTGACCCCGATCTACGACAATTTGGTTATGATAGCTCAAGGAGTCAATCAAGCACATCATCTTAA

Protein Analysis

752

Amino Acids

83.28

Weight (kDa)

8.64

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 14 - 151 6.7e-13 Universal stress protein family
Pkinase PF00069 436 - 684 2.1e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 436 - 689 8.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 1500
AccIII TCCGGA 1 cut(s) 1542
AciI CCGC 5 cut(s) 44, 507, 641, 1130, 2136
AclI AACGTT 1 cut(s) 570
AclWI GGATC 3 cut(s) 498, 1799, 2061
AcoI YGGCCR 3 cut(s) 48, 1365, 2101
AcsI RAATTY 1 cut(s) 1148
AfaI GTAC 8 cut(s) 215, 375, 956, 1247, 1256, 1487, 1508, 1650
AfeI AGCGCT 1 cut(s) 2097
AfiI CCNNNNNNNGG 4 cut(s) 1487, 1901, 2057, 2159
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 7 cut(s) 236, 582, 702, 1265, 1274, 1414, 1999
AjnI CCWGG 2 cut(s) 1666, 2006
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 2 cut(s) 1307, 1541
AlwI GGATC 3 cut(s) 498, 1799, 2061
Ama87I CYCGRG 3 cut(s) 623, 728, 1481
Aor13HI TCCGGA 1 cut(s) 1542
Aor51HI AGCGCT 1 cut(s) 2097
AoxI GGCC 8 cut(s) 48, 115, 1328, 1365, 1979, 1992, 2101, 2161
ApeKI GCWGC 8 cut(s) 396, 1109, 1121, 1277, 1391, 1437, 1573, 1592
ApoI RAATTY 1 cut(s) 1148
Asp700I GAANNNNTTC 1 cut(s) 697
AspLEI GCGC 1 cut(s) 2098
AspS9I GGNCC 5 cut(s) 620, 1328, 1459, 1684, 1941
AsuHPI GGTGA 4 cut(s) 669, 1355, 1750, 2150
AsuII TTCGAA 1 cut(s) 1585
AsuNHI GCTAGC 1 cut(s) 2011
AvaI CYCGRG 3 cut(s) 623, 728, 1481
AvaII GGWCC 4 cut(s) 620, 1459, 1684, 1941
BaeGI GKGCMC 1 cut(s) 451
BalI TGGCCA 3 cut(s) 50, 1367, 2103
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 2 cut(s) 830, 936
BbsI GAAGAC 1 cut(s) 1209
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 8 cut(s) 408, 1121, 1133, 1289, 1403, 1424, 1585, 1604
BccI CCATC 6 cut(s) 59, 394, 890, 1121, 1632, 1747
BcgI CGANNNNNNTGC 2 cut(s) 1049, 1083
BciT130I CCWGG 2 cut(s) 1668, 2008
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 1307, 1541
BfaI CTAG 4 cut(s) 929, 1349, 1725, 2012
BfmI CTRYAG 2 cut(s) 417, 1080
BfoI RGCGCY 1 cut(s) 2099
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 489
BisI GCNGC 9 cut(s) 397, 1110, 1122, 1131, 1278, 1392, 1438, 1574, 1593
BlsI GCNGC 9 cut(s) 398, 1111, 1123, 1132, 1279, 1393, 1439, 1575, 1594
Bme1390I CCNGG 2 cut(s) 1668, 2008
Bme18I GGWCC 4 cut(s) 620, 1459, 1684, 1941
BmeT110I CYCGRG 3 cut(s) 623, 728, 1481
BmgT120I GGNCC 5 cut(s) 620, 1328, 1459, 1684, 1941
BmiI GGNNCC 4 cut(s) 450, 622, 808, 1942
BmrFI CCNGG 2 cut(s) 1668, 2008
BmrI ACTGGG 1 cut(s) 1756
BmsI GCATC 2 cut(s) 646, 1378
BmtI GCTAGC 1 cut(s) 2015
BmuI ACTGGG 1 cut(s) 1756
BpiI GAAGAC 1 cut(s) 1209
Bpu10I CCTNAGC 2 cut(s) 1577, 2076
Bpu14I TTCGAA 1 cut(s) 1585
BpuEI CTTGAG 5 cut(s) 670, 920, 1289, 1380, 2212
BsaBI GATNNNNATC 1 cut(s) 412
BsaJI CCNNGG 3 cut(s) 435, 1059, 1568
BsaWI WCCGGW 3 cut(s) 1542, 2050, 2174
Bsc4I CCNNNNNNNGG 4 cut(s) 1487, 1901, 2057, 2159
Bse1I ACTGG 3 cut(s) 1762, 1828, 1982
Bse3DI GCAATG 4 cut(s) 128, 301, 518, 1077
Bse8I GATNNNNATC 1 cut(s) 412
BseAI TCCGGA 1 cut(s) 1542
BseBI CCWGG 2 cut(s) 1668, 2008
BseDI CCNNGG 3 cut(s) 435, 1059, 1568
BseGI GGATG 5 cut(s) 205, 637, 901, 1432, 1447
BseJI GATNNNNATC 1 cut(s) 412
BseLI CCNNNNNNNGG 4 cut(s) 1487, 1901, 2057, 2159
BseMI GCAATG 4 cut(s) 128, 301, 518, 1077
BseMII CTCAG 3 cut(s) 121, 186, 2067
BseNI ACTGG 3 cut(s) 1762, 1828, 1982
BseRI GAGGAG 2 cut(s) 931, 2003
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 8 cut(s) 408, 1121, 1133, 1289, 1403, 1424, 1585, 1604
BseYI CCCAGC 4 cut(s) 164, 602, 651, 1558
BsgI GTGCAG 1 cut(s) 1194
BshFI GGCC 8 cut(s) 50, 117, 1330, 1367, 1981, 1994, 2103, 2163
BshNI GGYRCC 1 cut(s) 448
BsiHKAI GWGCWC 1 cut(s) 936
BsiHKCI CYCGRG 3 cut(s) 623, 728, 1481
BsiSI CCGG 3 cut(s) 1543, 2051, 2175
BslFI GGGAC 4 cut(s) 962, 1464, 1718, 1954
BslI CCNNNNNNNGG 4 cut(s) 1487, 1901, 2057, 2159
BsmAI GTCTC 2 cut(s) 1307, 1541
BsmBI CGTCTC 1 cut(s) 1541
BsmFI GGGAC 4 cut(s) 962, 1464, 1718, 1954
BsmI GAATGC 1 cut(s) 1402
BsnI GGCC 8 cut(s) 50, 117, 1330, 1367, 1981, 1994, 2103, 2163
BsoBI CYCGRG 3 cut(s) 623, 728, 1481
Bsp119I TTCGAA 1 cut(s) 1585
Bsp1286I GDGCHC 3 cut(s) 451, 830, 936
Bsp13I TCCGGA 1 cut(s) 1542
Bsp1407I TGTACA 3 cut(s) 213, 954, 1648
Bsp143I GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
BspACI CCGC 5 cut(s) 44, 507, 641, 1130, 2136
BspANI GGCC 8 cut(s) 50, 117, 1330, 1367, 1981, 1994, 2103, 2163
BspCNI CTCAG 3 cut(s) 120, 187, 2068
BspEI TCCGGA 1 cut(s) 1542
BspLI GGNNCC 4 cut(s) 450, 622, 808, 1942
BspMAI CTGCAG 1 cut(s) 1084
BspOI GCTAGC 1 cut(s) 2015
BspPI GGATC 3 cut(s) 498, 1799, 2061
BspT104I TTCGAA 1 cut(s) 1585
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 4 cut(s) 128, 301, 518, 1077
BsrGI TGTACA 3 cut(s) 213, 954, 1648
BsrI ACTGG 3 cut(s) 1762, 1828, 1982
BssECI CCNNGG 3 cut(s) 435, 1059, 1568
BssMI GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
BssT1I CCWWGG 1 cut(s) 1568
Bst2UI CCWGG 2 cut(s) 1668, 2008
Bst4CI ACNGT 8 cut(s) 94, 436, 458, 466, 1410, 1490, 1655, 2022
Bst6I CTCTTC 7 cut(s) 20, 27, 1038, 1163, 1191, 1260, 1544
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 3 cut(s) 213, 954, 1648
BstBI TTCGAA 1 cut(s) 1585
BstC8I GCNNGC 4 cut(s) 967, 1634, 1829, 2013
BstDEI CTNAG 6 cut(s) 107, 195, 1525, 1577, 2076, 2121
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 5 cut(s) 205, 637, 901, 1432, 1447
BstH2I RGCGCY 1 cut(s) 2099
BstHHI GCGC 1 cut(s) 2098
BstKTI GATC 7 cut(s) 100, 492, 506, 1534, 1807, 2056, 2203
BstMAI GTCTC 2 cut(s) 1307, 1541
BstMBI GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
BstNI CCWGG 2 cut(s) 1668, 2008
BstNSI RCATGY 4 cut(s) 955, 1479, 1831, 1957
BstSCI CCNGG 2 cut(s) 1666, 2006
BstSFI CTRYAG 2 cut(s) 417, 1080
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 8 cut(s) 408, 1121, 1133, 1289, 1403, 1424, 1585, 1604
BstV2I GAAGAC 1 cut(s) 1209
BstX2I RGATCY 2 cut(s) 489, 2053
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstYI RGATCY 2 cut(s) 489, 2053
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 8 cut(s) 50, 117, 1330, 1367, 1981, 1994, 2103, 2163
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 5 cut(s) 205, 637, 901, 1432, 1447
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 4 cut(s) 45, 462, 1157, 2027
Cac8I GCNNGC 4 cut(s) 967, 1634, 1829, 2013
CfoI GCGC 1 cut(s) 2098
Cfr13I GGNCC 5 cut(s) 620, 1328, 1459, 1684, 1941
Csp6I GTAC 8 cut(s) 214, 374, 955, 1246, 1255, 1486, 1507, 1649
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviQI GTAC 8 cut(s) 214, 374, 955, 1246, 1255, 1486, 1507, 1649
DdeI CTNAG 6 cut(s) 107, 195, 1525, 1577, 2076, 2121
DpnI GATC 7 cut(s) 99, 491, 505, 1533, 1806, 2055, 2202
DpnII GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
DrdI GACNNNNNNGTC 1 cut(s) 190
DseDI GACNNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 3 cut(s) 48, 1365, 2101
Eam1104I CTCTTC 7 cut(s) 20, 27, 1038, 1163, 1191, 1260, 1544
EarI CTCTTC 7 cut(s) 20, 27, 1038, 1163, 1191, 1260, 1544
EciI GGCGGA 1 cut(s) 630
Ecl136II GAGCTC 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 1568
Eco147I AGGCCT 1 cut(s) 1994
Eco24I GRGCYC 2 cut(s) 830, 936
Eco47I GGWCC 4 cut(s) 620, 1459, 1684, 1941
Eco47III AGCGCT 1 cut(s) 2097
Eco53kI GAGCTC 1 cut(s) 934
Eco88I CYCGRG 3 cut(s) 623, 728, 1481
EcoICRI GAGCTC 1 cut(s) 934
EcoO109I RGGNCCY 2 cut(s) 620, 1941
EcoRI GAATTC 1 cut(s) 1148
EcoRII CCWGG 2 cut(s) 1666, 2006
EcoT14I CCWWGG 1 cut(s) 1568
EcoT38I GRGCYC 2 cut(s) 830, 936
ErhI CCWWGG 1 cut(s) 1568
Esp3I CGTCTC 1 cut(s) 1541
FaqI GGGAC 4 cut(s) 962, 1464, 1718, 1954
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 1500
Fnu4HI GCNGC 9 cut(s) 397, 1110, 1122, 1131, 1278, 1392, 1438, 1574, 1593
FokI GGATG 5 cut(s) 212, 624, 908, 1434, 1439
FriOI GRGCYC 2 cut(s) 830, 936
Fsp4HI GCNGC 9 cut(s) 397, 1110, 1122, 1131, 1278, 1392, 1438, 1574, 1593
FspBI CTAG 4 cut(s) 929, 1349, 1725, 2012
GlaI GCGC 1 cut(s) 2097
GluI GCNGC 9 cut(s) 397, 1110, 1122, 1131, 1278, 1392, 1438, 1574, 1593
GsaI CCCAGC 4 cut(s) 168, 606, 655, 1562
HaeII RGCGCY 1 cut(s) 2099
HaeIII GGCC 8 cut(s) 50, 117, 1330, 1367, 1981, 1994, 2103, 2163
HapII CCGG 3 cut(s) 1543, 2051, 2175
HhaI GCGC 1 cut(s) 2098
Hin6I GCGC 1 cut(s) 2096
HinP1I GCGC 1 cut(s) 2096
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 8 cut(s) 6, 333, 408, 578, 905, 1751, 1863, 2233
HpaII CCGG 3 cut(s) 1543, 2051, 2175
HphI GGTGA 4 cut(s) 669, 1355, 1750, 2150
Hpy166II GTNNAC 6 cut(s) 214, 1009, 1257, 1346, 1501, 1651
Hpy188I TCNGA 7 cut(s) 11, 196, 407, 577, 910, 1629, 1846
Hpy188III TCNNGA 9 cut(s) 174, 687, 730, 804, 1306, 1385, 1481, 1543, 1808
Hpy8I GTNNAC 6 cut(s) 214, 1009, 1257, 1346, 1501, 1651
HpyAV CCTTC 7 cut(s) 756, 1081, 1207, 1217, 1310, 1475, 2056
HpyCH4III ACNGT 8 cut(s) 94, 436, 458, 466, 1410, 1490, 1655, 2022
HpyCH4IV ACGT 3 cut(s) 423, 570, 1381
HpyF3I CTNAG 6 cut(s) 107, 195, 1525, 1577, 2076, 2121
HpySE526I ACGT 3 cut(s) 423, 570, 1381
HspAI GCGC 1 cut(s) 2096
Kpn2I TCCGGA 1 cut(s) 1542
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
LmnI GCTCC 1 cut(s) 1472
Lsp1109I GCAGC 8 cut(s) 408, 1121, 1133, 1289, 1403, 1424, 1585, 1604
LweI GCATC 2 cut(s) 646, 1378
MaeI CTAG 4 cut(s) 929, 1349, 1725, 2012
MaeII ACGT 3 cut(s) 423, 570, 1381
MaeIII GTNAC 5 cut(s) 675, 755, 1655, 1756, 2192
MalI GATC 7 cut(s) 99, 491, 505, 1533, 1806, 2055, 2202
MboI GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
MflI RGATCY 2 cut(s) 489, 2053
MhlI GDGCHC 3 cut(s) 451, 830, 936
MlsI TGGCCA 3 cut(s) 50, 1367, 2103
MluCI AATT 4 cut(s) 1148, 1587, 1599, 2210
MluNI TGGCCA 3 cut(s) 50, 1367, 2103
MlyI GAGTC 3 cut(s) 1745, 1872, 2242
MmeI TCCRAC 1 cut(s) 452
Mox20I TGGCCA 3 cut(s) 50, 1367, 2103
MroI TCCGGA 1 cut(s) 1542
MroXI GAANNNNTTC 1 cut(s) 697
MscI TGGCCA 3 cut(s) 50, 1367, 2103
MseI TTAA 6 cut(s) 141, 1191, 1662, 1971, 2082, 2254
MslI CAYNNNNRTG 2 cut(s) 821, 1692
Msp20I TGGCCA 3 cut(s) 50, 1367, 2103
MspI CCGG 3 cut(s) 1543, 2051, 2175
MspR9I CCNGG 2 cut(s) 1668, 2008
Mva1269I GAATGC 1 cut(s) 1402
MvaI CCWGG 2 cut(s) 1668, 2008
NdeI CATATG 1 cut(s) 496
NdeII GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
NheI GCTAGC 1 cut(s) 2011
NlaIV GGNNCC 4 cut(s) 450, 622, 808, 1942
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 4 cut(s) 675, 1655, 1756, 2192
NspI RCATGY 4 cut(s) 955, 1479, 1831, 1957
NspV TTCGAA 1 cut(s) 1585
PaeI GCATGC 1 cut(s) 1831
PaeR7I CTCGAG 1 cut(s) 728
PceI AGGCCT 1 cut(s) 1994
PciI ACATGT 1 cut(s) 951
PctI GAATGC 1 cut(s) 1402
PdmI GAANNNNTTC 1 cut(s) 697
PfeI GAWTC 5 cut(s) 6, 333, 408, 578, 905
PflFI GACNNNGTC 1 cut(s) 1754
PkrI GCNGC 9 cut(s) 398, 1111, 1123, 1132, 1279, 1393, 1439, 1575, 1594
PleI GAGTC 3 cut(s) 1745, 1871, 2241
PpsI GAGTC 3 cut(s) 1745, 1871, 2241
PpuMI RGGWCCY 2 cut(s) 620, 1941
PscI ACATGT 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 936
Psp1406I AACGTT 1 cut(s) 570
Psp5II RGGWCCY 2 cut(s) 620, 1941
Psp6I CCWGG 2 cut(s) 1666, 2006
PspFI CCCAGC 4 cut(s) 164, 602, 651, 1558
PspGI CCWGG 2 cut(s) 1666, 2006
PspN4I GGNNCC 4 cut(s) 450, 622, 808, 1942
PspPI GGNCC 5 cut(s) 620, 1328, 1459, 1684, 1941
PspPPI RGGWCCY 2 cut(s) 620, 1941
PsrI GAACNNNNNNTAC 2 cut(s) 1632, 1664
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 2 cut(s) 489, 2053
PsyI GACNNNGTC 1 cut(s) 1754
RsaI GTAC 8 cut(s) 215, 375, 956, 1247, 1256, 1487, 1508, 1650
RsaNI GTAC 8 cut(s) 214, 374, 955, 1246, 1255, 1486, 1507, 1649
RseI CAYNNNNRTG 2 cut(s) 821, 1692
SacI GAGCTC 1 cut(s) 936
SaqAI TTAA 6 cut(s) 141, 1191, 1662, 1971, 2082, 2254
SatI GCNGC 9 cut(s) 397, 1110, 1122, 1131, 1278, 1392, 1438, 1574, 1593
Sau3AI GATC 7 cut(s) 97, 489, 503, 1531, 1804, 2053, 2200
Sau96I GGNCC 5 cut(s) 620, 1328, 1459, 1684, 1941
SchI GAGTC 3 cut(s) 1745, 1872, 2242
ScrFI CCNGG 2 cut(s) 1668, 2008
SduI GDGCHC 3 cut(s) 451, 830, 936
SfaNI GCATC 2 cut(s) 646, 1378
SfcI CTRYAG 2 cut(s) 417, 1080
Sfr274I CTCGAG 1 cut(s) 728
SfuI TTCGAA 1 cut(s) 1585
SinI GGWCC 4 cut(s) 620, 1459, 1684, 1941
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 2 cut(s) 821, 1692
SmlI CTYRAG 6 cut(s) 685, 728, 935, 1304, 1395, 2227
SmoI CTYRAG 6 cut(s) 685, 728, 935, 1304, 1395, 2227
SphI GCATGC 1 cut(s) 1831
Sse9I AATT 4 cut(s) 1148, 1587, 1599, 2210
SseBI AGGCCT 1 cut(s) 1994
SsiI CCGC 5 cut(s) 44, 507, 641, 1130, 2136
SspI AATATT 1 cut(s) 1675
SspMI CTAG 4 cut(s) 929, 1349, 1725, 2012
SstI GAGCTC 1 cut(s) 936
StuI AGGCCT 1 cut(s) 1994
StyD4I CCNGG 2 cut(s) 1666, 2006
StyI CCWWGG 1 cut(s) 1568
TaaI ACNGT 8 cut(s) 94, 436, 458, 466, 1410, 1490, 1655, 2022
TaiI ACGT 3 cut(s) 426, 573, 1384
TaqI TCGA 7 cut(s) 54, 309, 539, 729, 1116, 1152, 1585
TasI AATT 4 cut(s) 1148, 1587, 1599, 2210
TatI WGTACW 5 cut(s) 213, 954, 1254, 1506, 1648
TauI GCSGC 1 cut(s) 1133
TfiI GAWTC 5 cut(s) 6, 333, 408, 578, 905
Tru1I TTAA 6 cut(s) 141, 1191, 1662, 1971, 2082, 2254
Tru9I TTAA 6 cut(s) 141, 1191, 1662, 1971, 2082, 2254
TscAI CASTG 4 cut(s) 45, 469, 1164, 2027
TseFI GTSAC 4 cut(s) 675, 1655, 1756, 2192
TseI GCWGC 8 cut(s) 396, 1109, 1121, 1277, 1391, 1437, 1573, 1592
Tsp45I GTSAC 4 cut(s) 675, 1655, 1756, 2192
TspDTI ATGAA 6 cut(s) 125, 312, 400, 719, 805, 1196
TspGWI ACGGA 2 cut(s) 251, 774
TspRI CASTG 4 cut(s) 45, 469, 1164, 2027
Tth111I GACNNNGTC 1 cut(s) 1754
VpaK11BI GGWCC 4 cut(s) 620, 1459, 1684, 1941
XapI RAATTY 1 cut(s) 1148
XceI RCATGY 4 cut(s) 955, 1479, 1831, 1957
XcmI CCANNNNNNNNNTGG 2 cut(s) 1566, 1897
XhoI CTCGAG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 1500
XmnI GAANNNNTTC 1 cut(s) 697
XspI CTAG 4 cut(s) 929, 1349, 1725, 2012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.