MD17G1224800.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000353
Physical Location & Seq
Reverse (-)
27328239 .. 27332316
4078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1224800.v1.1.491

Sequence Viewer

Length: 2259 bp
ATGTCGAGGATTGTTAGAGGGCACGGAGGAAAGAGAGGAGAGTCGGTGGCGGTGGCCGTAGACAAGGACAAAGGAAGTGAACATGCCATCAAATGGGCAGTTGATCATCTTCTCACCAGAGGCAAGCCTCTCACATTGCTCCATGTTAACCAAACAGGCTCCGACCCCCAACAAACCCAAGGGCTCTTTCTTTCTTTCCGTTCCTTGTGCTCCAAAAAGAATATAAAATGCAATGAAGTCGTAATCAAGGACACTGACATTCCAAACGCAATCATCGATTATGTTACGGCTAACACTGTTGAGATTTTGGTACTTGGCGTACCATCCAAAAGCGGCCTTTTCAGATTTAAGCTCACAGACGTTCCAAGCACAGTGTCAAAAGGGGCACCGGATTTTTGCTCTGTTTATGCGATTGGCAAAGGAAAGGTCTCGTACATGCGATGTGCTACTTCTGCACCCCAAAAATCTGCCCAACGTAATCAAGTACAACGCCAACCAAGCCCTATTTCTGAAGCCAGATCTTCAGCATTGAATAGGATGCAGCACTATTCTGCTCGTAACCAGCCAGATGGTGACATACAAATCAAGTCACCGTTCGCAAGAGGAAGACCCTCCATGGACAAATCATATGAGCTTTCACCAGAAACTGATATATCATTTGTAAGCAGTGGAAGGCCAAGCATTGATCGCATGTCACCTCGCTGTGACTTTGGTGTAGAATCAGGAATGAACTCTCGACTTTCAATCGGCTCAGACGGGGACAACAGAAGCTCCACATCGTCTTACTCAAGACACAAGTTTTTAGATATGGGCTCTTTACAATATGAAATGTCTTCATGCTCAACCGACGGTGGAAAATCATGGTCATCGTCACAAAACATGGACGATGTGGAAGCTGAGATGAGGAGGCTCAAGCTAGAGCTTAAGCAAACAATGGAAATGTACAGTACAGCGTGCAAGGAAGCTATGACAGCGAAAGACAAGGAAAAAGAAATTAACAGGTGGAAAATGGAAGATGAACAAAGGCTAGAAGCAGCGCGACATGCTGAGGAAGCTGCATTGGCACTTGTGGAGAGGGAGAAAGCAAAATGTAAGGCAGCCATTGAGGCAGCTAAAGCAGCTCAAAGGATTGCTGAATTGGAAGCACAAAAAAGAAAAGTTGCAGAAATGAAAGCCCATAAAGAATCCGAAGAGAAGAACAAGGCACTTCAAGCAAGGTCATATGATCTTAGGTACAGGAAATACACAATTCAGGAGATTGAATCGGCAACAAATGATTTCTCACAAGCTCGAAAAATCGGTGAAGGAGGCTATGGACCAGTGTACAGAGGTGAACTAGACCACACACCAGTGGCAATAAAAGTTCTACGTCCAGATGCAGCTCAAGGACAGTCGCAGTTCAAGCAGGAGGTTGAAGTATTAAGCAGTATACGACATCCAAACATGGTTCTCCTCCTTGGAGCTTGTCCGGAGTACGGTTGCTTGGTCTACGAGTACATGTCTAATGGGAGCTTGGAAGATCGTCTCTTCCGCAGAGGTAACACCCCAGTTATTCCTTGGCAGATAAGGTTCCGAATCGCTGCAGAAATCGGGACAGGGCTTTTGTTCTTTCATCAAGCCAAGCCAGAACCCCTTGTACATCGTGACCTAAAACCTGGAAACATCTTGCTAGACCATAACTATGTGAGCAAGATCAGTGATGTAGGTTTAGCTAGGCTTGTCCCTCCATCTGTAGCCAACTCTATCACTCAGTATCACATGACATCAACAGCCGGAACATTTTGCTACATTGACCCAGAGTATCAGCAAACTGGCATGCTTGGGACAAAATCTGATATCTACTCACTTGGCGTCATGCTTCTACAACTAATAACAGCCAAGCCACCAATGGGGTTGACTCATCACGTTGAGCGGGCTATTGAGACGGGAACTTTTGCTGATATGCTTGACCCTGCTGTTCCTGACTGGCCAGTTGAGGAAGCCTTGAAGTTTGCCAAGCTTTGTTTACAATGTGCTGAAATGAGGCGAAAGGACAGACCAGATCTTGGCAAGGTTGTGTTGCCTGAACTTAACAGATTGAGAGCACTCGCGGACGAGAGCTTCAATTGTGTCATGTTTGGTGCTGGTGGAGTATTTTCACCGAGACAACGCTCGAATCCCTCAATGCAAGATGTGATAAGTCAACAATCCGGATGTGAAAGCTCAAGGAGCCGTTCAAGCACATCATCATTTCCTGGAAAGATGGAGTTCTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

753

Amino Acids

83.53

Weight (kDa)

8.8

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 16 - 130 1.1e-08 Universal stress protein family
Pkinase PF00069 428 - 676 4.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 429 - 685 3.6e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 385
AccB7I CCANNNNNTGG 2 cut(s) 93, 2045
AccBSI CCGCTC 1 cut(s) 1912
AccI GTMKAC 3 cut(s) 60, 1429, 1488
AccII CGCG 2 cut(s) 1039, 2090
AccIII TCCGGA 2 cut(s) 1468, 2189
AciI CCGC 5 cut(s) 50, 333, 1531, 1912, 2090
AcoI YGGCCR 2 cut(s) 54, 1967
AcuI CTGAAG 2 cut(s) 507, 531
AcyI GRCGYC 1 cut(s) 1851
AfiI CCNNNNNNNGG 4 cut(s) 93, 1475, 1889, 2045
AflII CTTAAG 1 cut(s) 923
AflIII ACRYGT 1 cut(s) 1497
AgsI TTSAA 9 cut(s) 532, 744, 1211, 1262, 1402, 1415, 1987, 2104, 2217
AjnI CCWGG 2 cut(s) 1654, 2233
AjuI GAANNNNNNNTTGG 4 cut(s) 171, 203, 490, 522
AleI CACNNNNGTG 1 cut(s) 1349
Alw21I GWGCWC 2 cut(s) 212, 2086
Alw26I GTCTC 4 cut(s) 433, 1529, 1916, 2137
AlwNI CAGNNNCTG 1 cut(s) 647
Aor13HI TCCGGA 2 cut(s) 1468, 2189
AoxI GGCC 4 cut(s) 54, 334, 674, 1967
ApeKI GCWGC 8 cut(s) 541, 1034, 1055, 1097, 1109, 1118, 1379, 1580
ArsI GACNNNNNNTTYG 4 cut(s) 411, 443, 1585, 1617
AspLEI GCGC 1 cut(s) 1039
AspS9I GGNCC 1 cut(s) 1316
AsuHPI GGTGA 8 cut(s) 106, 582, 584, 630, 687, 1313, 1343, 2130
AvaII GGWCC 1 cut(s) 1316
BaeGI GKGCMC 2 cut(s) 24, 388
BalI TGGCCA 1 cut(s) 1969
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 2 cut(s) 186, 815
BbsI GAAGAC 2 cut(s) 613, 825
Bbv12I GWGCWC 2 cut(s) 212, 2086
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 8 cut(s) 553, 1042, 1046, 1109, 1121, 1130, 1391, 1567
BccI CCATC 5 cut(s) 95, 331, 563, 1735, 2236
BceAI ACGGC 3 cut(s) 41, 303, 2196
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 2 cut(s) 1656, 2235
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 4 cut(s) 433, 1529, 1916, 2137
BfaI CTAG 5 cut(s) 917, 1028, 1337, 1670, 1713
BfmI CTRYAG 2 cut(s) 1581, 1731
BfrI CTTAAG 1 cut(s) 923
BglI GCCNNNNNGGC 1 cut(s) 1106
BglII AGATCT 2 cut(s) 518, 2041
BisI GCNGC 9 cut(s) 334, 542, 1035, 1056, 1098, 1110, 1119, 1380, 1581
BlsI GCNGC 9 cut(s) 335, 543, 1036, 1057, 1099, 1111, 1120, 1381, 1582
Bme1390I CCNGG 2 cut(s) 1656, 2235
Bme18I GGWCC 1 cut(s) 1316
BmgT120I GGNCC 1 cut(s) 1316
BmiI GGNNCC 4 cut(s) 160, 387, 1571, 2210
BmrFI CCNGG 2 cut(s) 1656, 2235
BmrI ACTGGG 1 cut(s) 1541
BmsI GCATC 2 cut(s) 528, 1366
BmuI ACTGGG 1 cut(s) 1541
BpiI GAAGAC 2 cut(s) 613, 825
Bpu10I CCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 5 cut(s) 772, 896, 1368, 2188, 2236
Bsa29I ATCGAT 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 1851
BsaI GGTCTC 1 cut(s) 433
BsaJI CCNNGG 4 cut(s) 178, 615, 1456, 1556
BsaWI WCCGGW 3 cut(s) 388, 1468, 2189
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 755, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 1475, 1889, 2045
Bse1I ACTGG 6 cut(s) 1319, 1349, 1547, 1816, 1970, 1970
Bse3DI GCAATG 2 cut(s) 134, 238
BseAI TCCGGA 2 cut(s) 1468, 2189
BseBI CCWGG 2 cut(s) 1656, 2235
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 4 cut(s) 178, 615, 1456, 1556
BseGI GGATG 4 cut(s) 323, 543, 1435, 2198
BseLI CCNNNNNNNGG 4 cut(s) 93, 1475, 1889, 2045
BseMI GCAATG 2 cut(s) 134, 238
BseMII CTCAG 4 cut(s) 765, 888, 1038, 1763
BseNI ACTGG 6 cut(s) 1319, 1349, 1547, 1816, 1970, 1970
BseRI GAGGAG 3 cut(s) 51, 919, 1442
BseSI GKGCMC 2 cut(s) 24, 388
BseXI GCAGC 8 cut(s) 553, 1042, 1046, 1109, 1121, 1130, 1391, 1567
BsgI GTGCAG 1 cut(s) 438
Bsh1236I CGCG 2 cut(s) 1039, 2090
BshFI GGCC 4 cut(s) 56, 336, 676, 1969
BshNI GGYRCC 1 cut(s) 385
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 212, 2086
BsiSI CCGG 4 cut(s) 389, 1469, 1773, 2190
BslFI GGGAC 4 cut(s) 773, 1606, 1706, 1837
BslI CCNNNNNNNGG 4 cut(s) 93, 1475, 1889, 2045
BsmAI GTCTC 4 cut(s) 433, 1529, 1916, 2137
BsmBI CGTCTC 2 cut(s) 1529, 1916
BsmFI GGGAC 4 cut(s) 773, 1606, 1706, 1837
BsnI GGCC 4 cut(s) 56, 336, 676, 1969
Bso31I GGTCTC 1 cut(s) 433
Bsp1286I GDGCHC 6 cut(s) 24, 186, 212, 388, 815, 2086
Bsp13I TCCGGA 2 cut(s) 1468, 2189
Bsp1407I TGTACA 3 cut(s) 942, 1323, 1636
Bsp143I GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
Bsp19I CCATGG 1 cut(s) 615
BspACI CCGC 5 cut(s) 50, 333, 1531, 1912, 2090
BspANI GGCC 4 cut(s) 56, 336, 676, 1969
BspCNI CTCAG 4 cut(s) 764, 889, 1039, 1762
BspDI ATCGAT 1 cut(s) 276
BspEI TCCGGA 2 cut(s) 1468, 2189
BspFNI CGCG 2 cut(s) 1039, 2090
BspLI GGNNCC 4 cut(s) 160, 387, 1571, 2210
BspMAI CTGCAG 1 cut(s) 1585
BspT107I GGYRCC 1 cut(s) 385
BspTI CTTAAG 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 433
BsrBI CCGCTC 1 cut(s) 1912
BsrDI GCAATG 2 cut(s) 134, 238
BsrGI TGTACA 3 cut(s) 942, 1323, 1636
BsrI ACTGG 6 cut(s) 1319, 1349, 1547, 1816, 1970, 1970
BssECI CCNNGG 4 cut(s) 178, 615, 1456, 1556
BssMI GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
BssNAI GTATAC 1 cut(s) 1430
BssNI GRCGYC 1 cut(s) 1851
BssT1I CCWWGG 4 cut(s) 178, 615, 1456, 1556
Bst1107I GTATAC 1 cut(s) 1430
Bst2UI CCWGG 2 cut(s) 1656, 2235
Bst4CI ACNGT 7 cut(s) 298, 373, 594, 851, 947, 1392, 1478
Bst6I CTCTTC 2 cut(s) 1185, 1532
BstACI GRCGYC 1 cut(s) 1851
BstAFI CTTAAG 1 cut(s) 923
BstAUI TGTACA 3 cut(s) 942, 1323, 1636
BstC8I GCNNGC 4 cut(s) 125, 955, 1817, 1914
BstDEI CTNAG 5 cut(s) 751, 897, 1047, 1229, 1749
BstDSI CCRYGG 1 cut(s) 615
BstF5I GGATG 4 cut(s) 323, 543, 1435, 2198
BstFNI CGCG 2 cut(s) 1039, 2090
BstHHI GCGC 1 cut(s) 1039
BstKTI GATC 7 cut(s) 106, 521, 688, 1228, 1522, 1695, 2044
BstMAI GTCTC 4 cut(s) 433, 1529, 1916, 2137
BstMBI GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
BstNI CCWGG 2 cut(s) 1656, 2235
BstNSI RCATGY 6 cut(s) 86, 439, 694, 1046, 1501, 1819
BstSCI CCNGG 2 cut(s) 1654, 2233
BstSFI CTRYAG 2 cut(s) 1581, 1731
BstSLI GKGCMC 2 cut(s) 24, 388
BstUI CGCG 2 cut(s) 1039, 2090
BstV1I GCAGC 8 cut(s) 553, 1042, 1046, 1109, 1121, 1130, 1391, 1567
BstV2I GAAGAC 2 cut(s) 613, 825
BstX2I RGATCY 2 cut(s) 518, 2041
BstXI CCANNNNNNTGG 1 cut(s) 569
BstYI RGATCY 2 cut(s) 518, 2041
BstZ17I GTATAC 1 cut(s) 1430
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 4 cut(s) 56, 336, 676, 1969
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 615
BtgZI GCGATG 1 cut(s) 454
BtsCI GGATG 4 cut(s) 323, 543, 1435, 2198
BtsI GCAGTG 1 cut(s) 673
BtsIMutI CAGTG 7 cut(s) 252, 294, 378, 673, 1326, 1356, 1702
Cac8I GCNNGC 4 cut(s) 125, 955, 1817, 1914
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 1039
Cfr13I GGNCC 1 cut(s) 1316
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 1840
DdeI CTNAG 5 cut(s) 751, 897, 1047, 1229, 1749
DpnI GATC 7 cut(s) 105, 520, 687, 1227, 1521, 1694, 2043
DpnII GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
EaeI YGGCCR 2 cut(s) 54, 1967
Eam1104I CTCTTC 2 cut(s) 1185, 1532
EarI CTCTTC 2 cut(s) 1185, 1532
Eco130I CCWWGG 4 cut(s) 178, 615, 1456, 1556
Eco24I GRGCYC 2 cut(s) 186, 815
Eco31I GGTCTC 1 cut(s) 433
Eco32I GATATC 1 cut(s) 1837
Eco47I GGWCC 1 cut(s) 1316
Eco57I CTGAAG 2 cut(s) 507, 531
EcoRII CCWGG 2 cut(s) 1654, 2233
EcoRV GATATC 1 cut(s) 1837
EcoT14I CCWWGG 4 cut(s) 178, 615, 1456, 1556
EcoT38I GRGCYC 2 cut(s) 186, 815
ErhI CCWWGG 4 cut(s) 178, 615, 1456, 1556
Esp3I CGTCTC 2 cut(s) 1529, 1916
FaqI GGGAC 4 cut(s) 773, 1606, 1706, 1837
FauI CCCGC 1 cut(s) 1905
FauNDI CATATG 2 cut(s) 628, 1222
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 3 cut(s) 60, 1429, 1488
Fnu4HI GCNGC 9 cut(s) 334, 542, 1035, 1056, 1098, 1110, 1119, 1380, 1581
FokI GGATG 4 cut(s) 310, 550, 1422, 2205
FriOI GRGCYC 2 cut(s) 186, 815
Fsp4HI GCNGC 9 cut(s) 334, 542, 1035, 1056, 1098, 1110, 1119, 1380, 1581
FspBI CTAG 5 cut(s) 917, 1028, 1337, 1670, 1713
GlaI GCGC 1 cut(s) 1038
GluI GCNGC 9 cut(s) 334, 542, 1035, 1056, 1098, 1110, 1119, 1380, 1581
HaeIII GGCC 4 cut(s) 56, 336, 676, 1969
HapII CCGG 4 cut(s) 389, 1469, 1773, 2190
HgaI GACGC 1 cut(s) 1840
HhaI GCGC 1 cut(s) 1039
Hin1I GRCGYC 1 cut(s) 1851
Hin6I GCGC 1 cut(s) 1037
HinP1I GCGC 1 cut(s) 1037
HincII GTYRAC 3 cut(s) 148, 1896, 2183
HindII GTYRAC 3 cut(s) 148, 1896, 2183
HindIII AAGCTT 1 cut(s) 1997
HinfI GANTC 7 cut(s) 41, 719, 1184, 1262, 1575, 1897, 2155
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 4 cut(s) 389, 1469, 1773, 2190
HphI GGTGA 8 cut(s) 106, 582, 584, 630, 687, 1313, 1343, 2130
Hpy188I TCNGA 7 cut(s) 163, 344, 511, 754, 1189, 1574, 1834
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 2 cut(s) 666, 1298
HpyCH4III ACNGT 7 cut(s) 298, 373, 594, 851, 947, 1392, 1478
HpyCH4IV ACGT 4 cut(s) 360, 475, 1369, 1905
HpyCH4V TGCA 9 cut(s) 231, 455, 541, 957, 1058, 1163, 1379, 1583, 2167
HpyF3I CTNAG 5 cut(s) 751, 897, 1047, 1229, 1749
HpySE526I ACGT 4 cut(s) 360, 475, 1369, 1905
Hsp92I GRCGYC 1 cut(s) 1851
HspAI GCGC 1 cut(s) 1037
Kpn2I TCCGGA 2 cut(s) 1468, 2189
Ksp22I TGATCA 1 cut(s) 103
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
LmnI GCTCC 7 cut(s) 144, 164, 215, 776, 1460, 1509, 2208
Lsp1109I GCAGC 8 cut(s) 553, 1042, 1046, 1109, 1121, 1130, 1391, 1567
LweI GCATC 2 cut(s) 528, 1366
MaeI CTAG 5 cut(s) 917, 1028, 1337, 1670, 1713
MaeII ACGT 4 cut(s) 360, 475, 1369, 1905
MaeIII GTNAC 9 cut(s) 283, 557, 572, 588, 693, 704, 870, 1538, 1643
MalI GATC 7 cut(s) 105, 520, 687, 1227, 1521, 1694, 2043
MbiI CCGCTC 1 cut(s) 1912
MboI GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
MboII GAAGA 9 cut(s) 101, 513, 618, 825, 1025, 1202, 1207, 1519, 1529
MfeI CAATTG 1 cut(s) 2104
MflI RGATCY 2 cut(s) 518, 2041
MhlI GDGCHC 6 cut(s) 24, 186, 212, 388, 815, 2086
MlsI TGGCCA 1 cut(s) 1969
MluCI AATT 4 cut(s) 993, 1136, 1248, 2104
MluNI TGGCCA 1 cut(s) 1969
MlyI GAGTC 2 cut(s) 50, 1891
MmeI TCCRAC 1 cut(s) 186
Mox20I TGGCCA 1 cut(s) 1969
MroI TCCGGA 2 cut(s) 1468, 2189
MscI TGGCCA 1 cut(s) 1969
MseI TTAA 6 cut(s) 147, 348, 924, 996, 1421, 2070
MslI CAYNNNNRTG 2 cut(s) 1349, 1680
Msp20I TGGCCA 1 cut(s) 1969
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 4 cut(s) 389, 1469, 1773, 2190
MspR9I CCNGG 2 cut(s) 1656, 2235
MunI CAATTG 1 cut(s) 2104
MvaI CCWGG 2 cut(s) 1656, 2235
MvnI CGCG 2 cut(s) 1039, 2090
NcoI CCATGG 1 cut(s) 615
NdeI CATATG 2 cut(s) 628, 1222
NdeII GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
NlaIV GGNNCC 4 cut(s) 160, 387, 1571, 2210
NmuCI GTSAC 6 cut(s) 572, 588, 693, 704, 870, 1643
NspI RCATGY 6 cut(s) 86, 439, 694, 1046, 1501, 1819
OliI CACNNNNGTG 1 cut(s) 1349
PaeI GCATGC 1 cut(s) 1819
PciI ACATGT 1 cut(s) 1497
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 5 cut(s) 719, 1184, 1262, 1575, 2155
PflMI CCANNNNNTGG 2 cut(s) 93, 2045
PfoI TCCNGGA 1 cut(s) 2233
PkrI GCNGC 9 cut(s) 335, 543, 1036, 1057, 1099, 1111, 1120, 1381, 1582
PleI GAGTC 2 cut(s) 49, 1891
PpsI GAGTC 2 cut(s) 49, 1891
PscI ACATGT 1 cut(s) 1497
Psp6I CCWGG 2 cut(s) 1654, 2233
PspGI CCWGG 2 cut(s) 1654, 2233
PspN4I GGNNCC 4 cut(s) 160, 387, 1571, 2210
PspPI GGNCC 1 cut(s) 1316
PsrI GAACNNNNNNTAC 2 cut(s) 1620, 1652
PstI CTGCAG 1 cut(s) 1585
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 2 cut(s) 518, 2041
RseI CAYNNNNRTG 2 cut(s) 1349, 1680
SaqAI TTAA 6 cut(s) 147, 348, 924, 996, 1421, 2070
SatI GCNGC 9 cut(s) 334, 542, 1035, 1056, 1098, 1110, 1119, 1380, 1581
Sau3AI GATC 7 cut(s) 103, 518, 685, 1225, 1519, 1692, 2041
Sau96I GGNCC 1 cut(s) 1316
SchI GAGTC 2 cut(s) 50, 1891
ScrFI CCNGG 2 cut(s) 1656, 2235
SduI GDGCHC 6 cut(s) 24, 186, 212, 388, 815, 2086
SfaNI GCATC 2 cut(s) 528, 1366
SfcI CTRYAG 2 cut(s) 1581, 1731
SinI GGWCC 1 cut(s) 1316
SmiMI CAYNNNNRTG 2 cut(s) 1349, 1680
SmlI CTYRAG 6 cut(s) 787, 911, 923, 1383, 2203, 2251
SmoI CTYRAG 6 cut(s) 787, 911, 923, 1383, 2203, 2251
SphI GCATGC 1 cut(s) 1819
Sse9I AATT 4 cut(s) 993, 1136, 1248, 2104
SsiI CCGC 5 cut(s) 50, 333, 1531, 1912, 2090
SspMI CTAG 5 cut(s) 917, 1028, 1337, 1670, 1713
StyD4I CCNGG 2 cut(s) 1654, 2233
StyI CCWWGG 4 cut(s) 178, 615, 1456, 1556
TaaI ACNGT 7 cut(s) 298, 373, 594, 851, 947, 1392, 1478
TaiI ACGT 4 cut(s) 363, 478, 1372, 1908
TaqI TCGA 5 cut(s) 5, 276, 736, 1291, 2153
TasI AATT 4 cut(s) 993, 1136, 1248, 2104
TatI WGTACW 6 cut(s) 484, 942, 947, 1323, 1494, 1636
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 5 cut(s) 719, 1184, 1262, 1575, 2155
Tru1I TTAA 6 cut(s) 147, 348, 924, 996, 1421, 2070
Tru9I TTAA 6 cut(s) 147, 348, 924, 996, 1421, 2070
TscAI CASTG 7 cut(s) 259, 301, 378, 673, 1326, 1356, 1702
TseFI GTSAC 6 cut(s) 572, 588, 693, 704, 870, 1643
TseI GCWGC 8 cut(s) 541, 1034, 1055, 1097, 1109, 1118, 1379, 1580
Tsp45I GTSAC 6 cut(s) 572, 588, 693, 704, 870, 1643
TspDTI ATGAA 7 cut(s) 249, 743, 825, 840, 1032, 1184, 1601
TspGWI ACGGA 2 cut(s) 39, 188
TspRI CASTG 7 cut(s) 259, 301, 378, 673, 1326, 1356, 1702
Van91I CCANNNNNTGG 2 cut(s) 93, 2045
Vha464I CTTAAG 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 1316
XceI RCATGY 6 cut(s) 86, 439, 694, 1046, 1501, 1819
XcmI CCANNNNNNNNNTGG 2 cut(s) 1554, 1885
XmiI GTMKAC 3 cut(s) 60, 1429, 1488
XspI CTAG 5 cut(s) 917, 1028, 1337, 1670, 1713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.