Rroxscaffold_4G00318390

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000353
Physical Location & Seq
Reverse (-)
44522739 .. 44530878
8140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318390.1

Sequence Viewer

Length: 2127 bp
ATGGCAAGATCACGTGCCACAAGTGATGAAAAGAGAGAAGAGGCTGTGGCGGTGGCAATAGACAAGGACAAGGGAAGTCAATATGCTCTCAAATGGACTGTTGAGCATCTTCTTGTCAAGGGCCAAAGTCTCACCCTTGTGCATGTTAAGCAATCAACACCAGGCCTTCCCAATCAAACTGAGAAATCGGTTTTTGGCTCTGAATTAAAGGAGGAAGGTATAAAATTGTGCAAAAGCCAATCTGATGCTGAATATAGCGACTTGTTTCTTCCACTTCGTACTTTTTGTAAGAAAAAGCTTGTAAGATACAACGAAGTAATAGTTGAGGTTCAAATTCTTTTGATTAATACAGCATCAGACAGGACAAGAACACAGTACCGGCACCATAGCGCTCGGACAGATCATGACACAGGAATCAAGACACCATTCACCAGAGGACAAGCTTCATCAGACAGATTATATGAGATCTCACCAGACAGCGATGTATCATTCACAAGTAGTGGAGGAAGACCAAGCATTGATCACATGTTTCCTTCTATATGCGGCAGTGTGGAATCAGGAATGCACTCTCAACAATCATTCAACTCAGACAACAGAAGATCTACCTCCAAACAATCCTACTCAAGAACCCAGTTCATTGATATGAACTCCTCATTATCCTCAACTGAGAGTGGAAAATCGTGGTCATCCCGAAATATGGAAGAAGTAGAGGAGGAGATGATGAGACTCAAGCTAGAGCTCAAGCAGACAATGGAGGTGTACAATACGGCCTGTAAGGAAACCCTAACATCAAAACAGAAGAAACTTGACCGATGGAAATGGGAAGAAGAACAAAGATTGAAAAGTGCACACCTAGGTCAGGAGTTTGCATTGGCACTTGTACAGAAAGAGGAAGCAAAATGTAGGGCAGCAATGGACGCAGAAAGAGCAGCTAAAAGGATTGCTGAATTGGAAGCAGAACAAAGGAGGAATTCAGAAATAAAGGGTCTGAAAGAAGCTGAAAAGAGGACGAACTCACTAGGAGGAAGGAGTTTTGATCCGCAGTACAGGAAATACACAATTGAAGAGATTAAAGCAGCAACGAACAGTTTTTCAAAAGCTCTTAAGATTGGAGAAGGTGGCTATGGGCCTGTGTATGTAGGTGAGCTGGACCACACACAAGTGGCAATAAAAGCTCTACGTCCAGATGCAGCTCAAGGACGTTCACAGTTCCAACAAGAGATTGAAGTTTTGAGCTGCATAAGACATCCAAACATGGTTCTCCTGCTTGGAGCCTGCCCAGAGTATGGTTGTCTGATCTATGAGTACATGGGTAATGGGAGCTTAGAAGATCGTCTGTTTCGACGAGGTAACACCCCAGTTATTCCTTGGCAGCTAAGGTTCCGAATCGCTGCAGAAATTGCCACGGGGCTTTTGTTCCTTCACCAGGCCAAGCCAGAACCCTTTGTACACCGTGACTTAAAGCCAGGAAACATTTTGCTAGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTCTGGCCAGGCTTGTCCCTCAATCAGTAGCTGACTCAGTCACTCAGTATCACAAGACATCAGCAGCTGGGACATTCTGTTATATCGACCCAGAGTATCAACAAACTGGCTTGCTTGGAACAAAATCTGATATCTACTCACTTGGGGTCATGCTTCTACAAATAATTACAGCCAAACCAGCAATGGGTTTGACTCATCTTGTTGAGCACGCGATTGAGATAGGGACCTTCGCTGAGATGCTTGACCCCGCTGTTCCTGACTGGCCTGTGGAGGAAGCCTTGAAGTTTGCCAAGTTAGCATTACAATGTGCTGAAATGAGGCGAAAAGACAGACCCGATCTTGGCAAGGTAAGAATGTCCTTAGCCAAGAAGGAGCGGACATCCGCAACCCGGAAAAGGTGCGAACGTTCCTCCTCCGACATTGAGAAGGCGGCGTTGGCTGCGGTGAGGGTGCCAAACGAACCCTCTGGACATCTTGGACGGTGTCACCATCAAGGTGAAGATGAAGGTGATCGAAATCTCGGCGATGTGCGGCGAGCTCGCCCGAAACCATGGAAACCCATCGAGGTCGACTTCGAAACCTTCATCGAAGAGTCCAGCCGCTTCGAATGA

Protein Analysis

708

Amino Acids

79.81

Weight (kDa)

8.27

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 366 - 617 3.6e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 369 - 624 5.7e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 381, 1966
AccB7I CCANNNNNTGG 1 cut(s) 1286
AccBSI CCGCTC 1 cut(s) 1891
AccI GTMKAC 1 cut(s) 2085
AccII CGCG 1 cut(s) 1727
AclI AACGTT 1 cut(s) 1921
AclWI GGATC 1 cut(s) 1031
AcoI YGGCCR 1 cut(s) 1521
AcsI RAATTY 2 cut(s) 333, 970
AcvI CACGTG 1 cut(s) 14
AfaI GTAC 7 cut(s) 280, 377, 761, 882, 1046, 1307, 1449
AfeI AGCGCT 1 cut(s) 391
AfiI CCNNNNNNNGG 8 cut(s) 697, 859, 1286, 1426, 1700, 1856, 1905, 1911
AflII CTTAAG 1 cut(s) 1103
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 7 cut(s) 332, 583, 841, 1064, 1095, 1226, 1798
AjnI CCWGG 4 cut(s) 160, 1425, 1465, 1523
AjuI GAANNNNNNNTTGG 2 cut(s) 1934, 1966
AleI CACNNNNGTG 2 cut(s) 137, 1160
Alw21I GWGCWC 4 cut(s) 741, 850, 1725, 2056
Alw26I GTCTC 2 cut(s) 134, 718
Alw44I GTGCAC 1 cut(s) 846
AlwI GGATC 1 cut(s) 1031
AlwNI CAGNNNCTG 2 cut(s) 1547, 1583
Aor51HI AGCGCT 1 cut(s) 391
AoxI GGCC 7 cut(s) 121, 163, 768, 1127, 1428, 1521, 1778
ApaLI GTGCAC 1 cut(s) 846
ApeKI GCWGC 9 cut(s) 908, 929, 1076, 1190, 1236, 1372, 1391, 1580, 1955
ApoI RAATTY 2 cut(s) 333, 970
AseI ATTAAT 1 cut(s) 345
AspA2I CCTAGG 1 cut(s) 853
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 4 cut(s) 121, 1127, 1150, 1740
AsuC2I CCSGG 1 cut(s) 1906
AsuHPI GGTGA 9 cut(s) 124, 421, 462, 1154, 1415, 1972, 1994, 2024, 2036
AsuII TTCGAA 2 cut(s) 2091, 2121
AvaII GGWCC 2 cut(s) 1150, 1740
AvrII CCTAGG 1 cut(s) 853
BaeGI GKGCMC 1 cut(s) 850
BalI TGGCCA 1 cut(s) 1523
BanI GGYRCC 2 cut(s) 381, 1966
BanII GRGCYC 2 cut(s) 741, 2056
BbrPI CACGTG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 514
Bbv12I GWGCWC 4 cut(s) 741, 850, 1725, 2056
BbvI GCAGC 9 cut(s) 920, 941, 1088, 1202, 1223, 1378, 1384, 1592, 1942
BccI CCATC 3 cut(s) 807, 2013, 2084
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 4 cut(s) 162, 1427, 1467, 1525
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 1906
BcoDI GTCTC 2 cut(s) 134, 718
BfaI CTAG 4 cut(s) 734, 854, 1019, 1481
BfmI CTRYAG 1 cut(s) 1392
BfoI RGCGCY 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 1103
BglII AGATCT 2 cut(s) 465, 599
BlnI CCTAGG 1 cut(s) 853
Bme1390I CCNGG 5 cut(s) 162, 1427, 1467, 1525, 1906
Bme18I GGWCC 2 cut(s) 1150, 1740
BmgT120I GGNCC 4 cut(s) 121, 1127, 1150, 1740
BmiI GGNNCC 5 cut(s) 383, 1273, 1382, 1741, 1968
BmrFI CCNGG 5 cut(s) 162, 1427, 1467, 1525, 1906
BmrI ACTGGG 2 cut(s) 625, 1352
BmsI GCATC 5 cut(s) 115, 235, 362, 1177, 1743
BmuI ACTGGG 2 cut(s) 625, 1352
BpiI GAAGAC 1 cut(s) 514
Bpu10I CCTNAGC 2 cut(s) 1376, 1876
Bpu14I TTCGAA 2 cut(s) 2091, 2121
BpuEI CTTGAG 4 cut(s) 607, 713, 725, 1179
BpuMI CCSGG 1 cut(s) 1906
BsaAI YACGTR 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 2031
BsaJI CCNNGG 4 cut(s) 853, 1367, 1404, 2066
Bsc4I CCNNNNNNNGG 8 cut(s) 697, 859, 1286, 1426, 1700, 1856, 1905, 1911
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 4 cut(s) 631, 1358, 1627, 1781
Bse3DI GCAATG 2 cut(s) 918, 1704
Bse8I GATNNNNATC 1 cut(s) 2031
BseBI CCWGG 4 cut(s) 162, 1427, 1467, 1525
BseDI CCNNGG 4 cut(s) 853, 1367, 1404, 2066
BseGI GGATG 3 cut(s) 686, 1246, 1895
BseJI GATNNNNATC 1 cut(s) 2031
BseLI CCNNNNNNNGG 8 cut(s) 697, 859, 1286, 1426, 1700, 1856, 1905, 1911
BseMI GCAATG 2 cut(s) 918, 1704
BseMII CTCAG 6 cut(s) 171, 600, 657, 1566, 1574, 1740
BseNI ACTGG 4 cut(s) 631, 1358, 1627, 1781
BseRI GAGGAG 4 cut(s) 640, 725, 728, 1918
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 9 cut(s) 920, 941, 1088, 1202, 1223, 1378, 1384, 1592, 1942
BseYI CCCAGC 1 cut(s) 1583
Bsh1236I CGCG 1 cut(s) 1727
BshFI GGCC 7 cut(s) 123, 165, 770, 1129, 1430, 1523, 1780
BshNI GGYRCC 2 cut(s) 381, 1966
BsiHKAI GWGCWC 4 cut(s) 741, 850, 1725, 2056
BsiSI CCGG 2 cut(s) 379, 1906
BslFI GGGAC 3 cut(s) 1517, 1600, 1753
BslI CCNNNNNNNGG 8 cut(s) 697, 859, 1286, 1426, 1700, 1856, 1905, 1911
BsmAI GTCTC 2 cut(s) 134, 718
BsmFI GGGAC 3 cut(s) 1517, 1600, 1753
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 7 cut(s) 123, 165, 770, 1129, 1430, 1523, 1780
Bsp119I TTCGAA 2 cut(s) 2091, 2121
Bsp1286I GDGCHC 4 cut(s) 741, 850, 1725, 2056
Bsp1407I TGTACA 3 cut(s) 759, 880, 1447
Bsp19I CCATGG 1 cut(s) 2066
BspANI GGCC 7 cut(s) 123, 165, 770, 1129, 1430, 1523, 1780
BspCNI CTCAG 6 cut(s) 172, 599, 658, 1565, 1573, 1741
BspFNI CGCG 1 cut(s) 1727
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 5 cut(s) 383, 1273, 1382, 1741, 1968
BspMAI CTGCAG 1 cut(s) 1396
BspPI GGATC 1 cut(s) 1031
BspT104I TTCGAA 2 cut(s) 2091, 2121
BspT107I GGYRCC 2 cut(s) 381, 1966
BspTI CTTAAG 1 cut(s) 1103
BsrBI CCGCTC 1 cut(s) 1891
BsrDI GCAATG 2 cut(s) 918, 1704
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 3 cut(s) 759, 880, 1447
BsrI ACTGG 4 cut(s) 631, 1358, 1627, 1781
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 4 cut(s) 853, 1367, 1404, 2066
BssT1I CCWWGG 3 cut(s) 853, 1367, 2066
Bst2UI CCWGG 4 cut(s) 162, 1427, 1467, 1525
Bst4CI ACNGT 6 cut(s) 100, 375, 1088, 1209, 1454, 1998
Bst6I CTCTTC 3 cut(s) 33, 1059, 2100
BstAFI CTTAAG 1 cut(s) 1103
BstAPI GCANNNNNTGC 1 cut(s) 1400
BstAUI TGTACA 3 cut(s) 759, 880, 1447
BstBAI YACGTR 1 cut(s) 14
BstBI TTCGAA 2 cut(s) 2091, 2121
BstC8I GCNNGC 5 cut(s) 1276, 1628, 1725, 2052, 2056
BstDEI CTNAG 9 cut(s) 180, 586, 666, 1324, 1376, 1552, 1560, 1749, 1876
BstDSI CCRYGG 2 cut(s) 1404, 2066
BstENI CCTNNNNNAGG 2 cut(s) 857, 1424
BstF5I GGATG 3 cut(s) 686, 1246, 1895
BstFNI CGCG 1 cut(s) 1727
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 1 cut(s) 392
BstMAI GTCTC 2 cut(s) 134, 718
BstMWI GCNNNNNNNGC 9 cut(s) 148, 917, 926, 1172, 1400, 1694, 1811, 1952, 1955
BstNI CCWGG 4 cut(s) 162, 1427, 1467, 1525
BstNSI RCATGY 2 cut(s) 146, 529
BstSCI CCNGG 5 cut(s) 160, 1425, 1465, 1523, 1904
BstSFI CTRYAG 1 cut(s) 1392
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 1727
BstV1I GCAGC 9 cut(s) 920, 941, 1088, 1202, 1223, 1378, 1384, 1592, 1942
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 2 cut(s) 465, 599
BstYI RGATCY 2 cut(s) 465, 599
BsuRI GGCC 7 cut(s) 123, 165, 770, 1129, 1430, 1523, 1780
BtgI CCRYGG 2 cut(s) 1404, 2066
BtgZI GCGATG 2 cut(s) 495, 2055
BtsCI GGATG 3 cut(s) 686, 1246, 1895
BtsI GCAGTG 1 cut(s) 553
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 5 cut(s) 1276, 1628, 1725, 2052, 2056
CaiI CAGNNNCTG 2 cut(s) 1547, 1583
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 4 cut(s) 121, 1127, 1150, 1740
CseI GACGC 1 cut(s) 926
Csp6I GTAC 7 cut(s) 279, 376, 760, 881, 1045, 1306, 1448
CviAII CATG 7 cut(s) 143, 404, 526, 1255, 1309, 1666, 2067
CviQI GTAC 7 cut(s) 279, 376, 760, 881, 1045, 1306, 1448
DdeI CTNAG 9 cut(s) 180, 586, 666, 1324, 1376, 1552, 1560, 1749, 1876
EaeI YGGCCR 1 cut(s) 1521
Eam1104I CTCTTC 3 cut(s) 33, 1059, 2100
EarI CTCTTC 3 cut(s) 33, 1059, 2100
Ecl136II GAGCTC 2 cut(s) 739, 2054
Eco130I CCWWGG 3 cut(s) 853, 1367, 2066
Eco147I AGGCCT 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 741, 2056
Eco32I GATATC 1 cut(s) 1648
Eco47I GGWCC 2 cut(s) 1150, 1740
Eco47III AGCGCT 1 cut(s) 391
Eco53kI GAGCTC 2 cut(s) 739, 2054
Eco72I CACGTG 1 cut(s) 14
EcoICRI GAGCTC 2 cut(s) 739, 2054
EcoNI CCTNNNNNAGG 2 cut(s) 857, 1424
EcoO109I RGGNCCY 1 cut(s) 1740
EcoRI GAATTC 1 cut(s) 970
EcoRII CCWGG 4 cut(s) 160, 1425, 1465, 1523
EcoRV GATATC 1 cut(s) 1648
EcoT14I CCWWGG 3 cut(s) 853, 1367, 2066
EcoT38I GRGCYC 2 cut(s) 741, 2056
ErhI CCWWGG 3 cut(s) 853, 1367, 2066
FaeI CATG 7 cut(s) 146, 407, 529, 1258, 1312, 1669, 2070
FaqI GGGAC 3 cut(s) 1517, 1600, 1753
FatI CATG 7 cut(s) 142, 403, 525, 1254, 1308, 1665, 2066
FauI CCCGC 1 cut(s) 1771
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 2085
FokI GGATG 3 cut(s) 673, 1233, 1882
FriOI GRGCYC 2 cut(s) 741, 2056
FspBI CTAG 4 cut(s) 734, 854, 1019, 1481
GlaI GCGC 1 cut(s) 391
GsaI CCCAGC 1 cut(s) 1587
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 7 cut(s) 123, 165, 770, 1129, 1430, 1523, 1780
HapII CCGG 2 cut(s) 379, 1906
HgaI GACGC 1 cut(s) 926
HhaI GCGC 1 cut(s) 392
Hin1II CATG 7 cut(s) 146, 407, 529, 1258, 1312, 1669, 2070
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 2086
HindII GTYRAC 1 cut(s) 2086
HindIII AAGCTT 2 cut(s) 296, 441
HinfI GANTC 7 cut(s) 414, 554, 726, 1386, 1550, 1708, 2108
HpaII CCGG 2 cut(s) 379, 1906
HphI GGTGA 9 cut(s) 124, 421, 462, 1154, 1415, 1972, 1994, 2024, 2036
Hpy166II GTNNAC 5 cut(s) 760, 848, 1205, 1450, 2086
Hpy188III TCNNGA 9 cut(s) 404, 418, 558, 624, 690, 860, 1184, 1772, 1983
Hpy8I GTNNAC 5 cut(s) 760, 848, 1205, 1450, 2086
Hpy99I CGWCG 1 cut(s) 1347
HpyCH4III ACNGT 6 cut(s) 100, 375, 1088, 1209, 1454, 1998
HpyCH4IV ACGT 4 cut(s) 13, 1180, 1201, 1921
HpyCH4V TGCA 8 cut(s) 142, 231, 565, 848, 869, 1190, 1239, 1394
HpyF10VI GCNNNNNNNGC 9 cut(s) 148, 917, 926, 1172, 1400, 1694, 1811, 1952, 1955
HpyF3I CTNAG 9 cut(s) 180, 586, 666, 1324, 1376, 1552, 1560, 1749, 1876
HpySE526I ACGT 4 cut(s) 13, 1180, 1201, 1921
Hsp92II CATG 7 cut(s) 146, 407, 529, 1258, 1312, 1669, 2070
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 520
LmnI GCTCC 3 cut(s) 1271, 1320, 1888
Lsp1109I GCAGC 9 cut(s) 920, 941, 1088, 1202, 1223, 1378, 1384, 1592, 1942
LweI GCATC 5 cut(s) 115, 235, 362, 1177, 1743
MaeI CTAG 4 cut(s) 734, 854, 1019, 1481
MaeII ACGT 4 cut(s) 13, 1180, 1201, 1921
MaeIII GTNAC 4 cut(s) 1349, 1454, 1555, 2000
MbiI CCGCTC 1 cut(s) 1891
MfeI CAATTG 1 cut(s) 1059
MflI RGATCY 2 cut(s) 465, 599
MhlI GDGCHC 4 cut(s) 741, 850, 1725, 2056
MlsI TGGCCA 1 cut(s) 1523
MluCI AATT 8 cut(s) 203, 224, 333, 947, 970, 1059, 1398, 1680
MluNI TGGCCA 1 cut(s) 1523
MlyI GAGTC 4 cut(s) 720, 1544, 1702, 2117
MmeI TCCRAC 2 cut(s) 1237, 1956
Mox20I TGGCCA 1 cut(s) 1523
MscI TGGCCA 1 cut(s) 1523
MseI TTAA 6 cut(s) 147, 206, 345, 1071, 1104, 1460
MslI CAYNNNNRTG 6 cut(s) 137, 641, 1160, 1491, 1819, 2010
Msp20I TGGCCA 1 cut(s) 1523
MspA1I CMGCKG 2 cut(s) 1583, 1766
MspCI CTTAAG 1 cut(s) 1103
MspI CCGG 2 cut(s) 379, 1906
MspR9I CCNGG 5 cut(s) 162, 1427, 1467, 1525, 1906
MunI CAATTG 1 cut(s) 1059
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 4 cut(s) 162, 1427, 1467, 1525
MvnI CGCG 1 cut(s) 1727
MwoI GCNNNNNNNGC 9 cut(s) 148, 917, 926, 1172, 1400, 1694, 1811, 1952, 1955
NciI CCSGG 1 cut(s) 1906
NcoI CCATGG 1 cut(s) 2066
NlaIII CATG 7 cut(s) 146, 407, 529, 1258, 1312, 1669, 2070
NlaIV GGNNCC 5 cut(s) 383, 1273, 1382, 1741, 1968
NmeAIII GCCGAG 1 cut(s) 2016
NmuCI GTSAC 3 cut(s) 1454, 1555, 2000
NspI RCATGY 2 cut(s) 146, 529
NspV TTCGAA 2 cut(s) 2091, 2121
OliI CACNNNNGTG 2 cut(s) 137, 1160
PagI TCATGA 1 cut(s) 403
PceI AGGCCT 1 cut(s) 165
PciI ACATGT 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PctI GAATGC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 414, 554, 1386
PflFI GACNNNGTC 2 cut(s) 1553, 1998
PflMI CCANNNNNTGG 1 cut(s) 1286
PleI GAGTC 4 cut(s) 720, 1544, 1702, 2116
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 4 cut(s) 720, 1544, 1702, 2116
Ppu21I YACGTR 1 cut(s) 14
PpuMI RGGWCCY 1 cut(s) 1740
PscI ACATGT 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 741, 2056
Psp1406I AACGTT 1 cut(s) 1921
Psp5II RGGWCCY 1 cut(s) 1740
Psp6I CCWGG 4 cut(s) 160, 1425, 1465, 1523
PspCI CACGTG 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 1583
PspGI CCWGG 4 cut(s) 160, 1425, 1465, 1523
PspN4I GGNNCC 5 cut(s) 383, 1273, 1382, 1741, 1968
PspPI GGNCC 4 cut(s) 121, 1127, 1150, 1740
PspPPI RGGWCCY 1 cut(s) 1740
PsrI GAACNNNNNNTAC 2 cut(s) 1431, 1463
PstI CTGCAG 1 cut(s) 1396
PstNI CAGNNNCTG 2 cut(s) 1547, 1583
PsuI RGATCY 2 cut(s) 465, 599
PsyI GACNNNGTC 2 cut(s) 1553, 1998
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 7 cut(s) 280, 377, 761, 882, 1046, 1307, 1449
RsaNI GTAC 7 cut(s) 279, 376, 760, 881, 1045, 1306, 1448
RseI CAYNNNNRTG 6 cut(s) 137, 641, 1160, 1491, 1819, 2010
SacI GAGCTC 2 cut(s) 741, 2056
SalI GTCGAC 1 cut(s) 2084
SaqAI TTAA 6 cut(s) 147, 206, 345, 1071, 1104, 1460
Sau96I GGNCC 4 cut(s) 121, 1127, 1150, 1740
SchI GAGTC 4 cut(s) 720, 1544, 1702, 2117
ScrFI CCNGG 5 cut(s) 162, 1427, 1467, 1525, 1906
SduI GDGCHC 4 cut(s) 741, 850, 1725, 2056
SfaNI GCATC 5 cut(s) 115, 235, 362, 1177, 1743
SfcI CTRYAG 1 cut(s) 1392
SfuI TTCGAA 2 cut(s) 2091, 2121
SinI GGWCC 2 cut(s) 1150, 1740
SmiMI CAYNNNNRTG 6 cut(s) 137, 641, 1160, 1491, 1819, 2010
SmlI CTYRAG 5 cut(s) 622, 728, 740, 1103, 1194
SmoI CTYRAG 5 cut(s) 622, 728, 740, 1103, 1194
Sse9I AATT 8 cut(s) 203, 224, 333, 947, 970, 1059, 1398, 1680
SseBI AGGCCT 1 cut(s) 165
SspMI CTAG 4 cut(s) 734, 854, 1019, 1481
SstI GAGCTC 2 cut(s) 741, 2056
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 5 cut(s) 160, 1425, 1465, 1523, 1904
StyI CCWWGG 3 cut(s) 853, 1367, 2066
TaaI ACNGT 6 cut(s) 100, 375, 1088, 1209, 1454, 1998
TaiI ACGT 4 cut(s) 16, 1183, 1204, 1924
TaqI TCGA 8 cut(s) 1342, 1602, 2029, 2079, 2085, 2091, 2103, 2121
TaqII GACCGA 1 cut(s) 825
TasI AATT 8 cut(s) 203, 224, 333, 947, 970, 1059, 1398, 1680
TatI WGTACW 5 cut(s) 759, 880, 1044, 1305, 1447
TauI GCSGC 4 cut(s) 546, 1949, 2050, 2118
TfiI GAWTC 3 cut(s) 414, 554, 1386
Tru1I TTAA 6 cut(s) 147, 206, 345, 1071, 1104, 1460
Tru9I TTAA 6 cut(s) 147, 206, 345, 1071, 1104, 1460
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 3 cut(s) 1454, 1555, 2000
TseI GCWGC 9 cut(s) 908, 929, 1076, 1190, 1236, 1372, 1391, 1580, 1955
Tsp45I GTSAC 3 cut(s) 1454, 1555, 2000
TspDTI ATGAA 6 cut(s) 42, 435, 625, 659, 2034, 2089
TspRI CASTG 1 cut(s) 553
Tth111I GACNNNGTC 2 cut(s) 1553, 1998
Van91I CCANNNNNTGG 1 cut(s) 1286
Vha464I CTTAAG 1 cut(s) 1103
VneI GTGCAC 1 cut(s) 846
VpaK11BI GGWCC 2 cut(s) 1150, 1740
VspI ATTAAT 1 cut(s) 345
XagI CCTNNNNNAGG 2 cut(s) 857, 1424
XapI RAATTY 2 cut(s) 333, 970
XceI RCATGY 2 cut(s) 146, 529
XcmI CCANNNNNNNNNTGG 2 cut(s) 1365, 1696
XmaJI CCTAGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 2085
XspI CTAG 4 cut(s) 734, 854, 1019, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.