RchiOBHm_Chr2g0132681

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000353
Physical Location & Seq
Forward (+)
49281012 .. 49285990
4979 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50392

Sequence Viewer

Length: 2232 bp
ATGAGAGGTAGAGGGCACGACGCAGAACAACAATTAAAAGAACAGGCAGTGGCAGTGGCCATAGACAAGGACAAAGGAAGTGAATATGCAATCAAATGGGCTATTGATAGTCTCTTAACCAAGGGCCAACCTCTCACATTGCTCCATGTTAGACATCCATATCCTGGCCGGACTGCGTCAACGGAGAATCAAAAAGATCCCCAATCACATGAGTTATTTCTTTCGTTTCGTTCCTTTTGCGCGAAAAAAAATATAAAATGCACTGAAGTCATACTCGAGGACAATGACATACCAAACGCACTGATCAAGTATGTTAATGCAAATTCAATTGAGATTTTGGTACTTGGTGTTCCAACAAGAGGTGGCATTTTCAGTGTGATAAAAATGACGCCAGAATTTTGCTCTGTTTATGTCATCGCTAAAGGAAAAATCTCATCTATGCGATGCTCCACCGTAGCACCGCCACAGCCTCCTCCACGTAGTAATCATCAAATGCAACACCATCCCAGCAATGTTTCTGAAGCAAGTGATTCGCATTTCATCTGCACCCAGAAGCCTAACAGCAGAGCATCAGAGAGGTTGCATTATCAATCGGGTAGCCAGCAGTTTGATGAAATCAAGTCACCATTCACTAGAGGAAGACCTTCGATGGACAGATCATTTGAGCTCCCACCAGATAGTGACATATCATTTGTGAGTACTGGAAGACCAAGTGTGGATCGCATGTATCCTTCTTTCTATGATAGTATGGATTCAGGGAGGAACTCTCGACGTTTGATTGGCTCAGAAACGGAAAACAGAAGCTCTGTGTCATCCAACCCGAGAAGCAAGCATTTTATGGAAATCAACTCTCCACAACTTGAATTTTCACCATCCTCAGTTGATAGTGGAAACTCTTGGTTGTCACAAAATAATAATAATAATTTGGAAGAGGTGGAGCTTGAGATGAGGAGGCTCAGGCTAGAGCTCAAGCAAACAATGGAGATGTACAATACAGCGTGCAAAGAAGCACTGACAGCAAAACATAAGGAAAATGAATTTAACCGGTGTAAAATGGAAGAAGGACAAAGATTAGAACAGGCGCGACTAGCTGAGGAAGCTGAATTGGCACTTGTAGAGAGGGAGAAAGCAAAATGCAAGGCAGCGATTGAAGCAGCTGCAGCAGCTCAAAGGCTTGCTGAACTGGAAGCGCAAAAGAGGAGGAACGCAGAAATGAAAGCCCTTAAAGAATCTGAAGAGAGGAAGAAGACACTTGAAGCAAAGTCATATGATGTTAGGTACAGGAAGTACACAATTGAGGAGATTGAAGTGGCAACAGATAAATTTTCACTAGCTCGCAAGGTTGGGGAAGGAGGCTATGGGCCAGTATACAGAGGTGAACTGCATCATACACCAGTTGCTATAAAAGTTCTACGTCCGGATGCAGCTCAAGGGCGATCGCAGTTCCAGCTAGAGGTTGAAGTATTGAGCTGCATACGACATCCAAACATGGTTCTCCTTCTTGGAGCTTGTCCAGAGTACGGTTGTTTAGTCTATGAGTACATGGCTAATGGGAGCTTGGAAAATCGTCTATTTCACCGAGGTAACACCCCGGCTATTCCTTGGCAACTAAGGTTTCGAATTGCTGCAGAAATTGCAACGGGGCTGTTATTCCTTCATCAGGCTAAGCCAGAACCCCTTGTGCACCGTGACTTAAAACCTGGCAACATATTGCTAGATAATAACTATGTGAGCAAGATTAGTGATGTAGGTTTGGCTAGACTTGTGCCTCCATCAATAGCTGATGCGGTCACACAGTATCATATGACATCAACAGCTGGAACATTTTGCTACATTGATCCAGAGTATCAGCAAACAGGCATGCTTGGAACAAAATCTGATATCTACTCACTAGGTGTGATGCTTCTACAAATCATTACAGCCAAACCACCAATGGGTTTGACTCATCATGTAGAGCGGGCTATTGAGACGGGAAGCTTTAGTGAGATGCTTGATCCTGCAGTTCCTGATTGGCCAGTTGAGGAAGCCTTGAAGTTTGCCAAACTTTCTTTACAGTGTGCTGAAATGAGAAAAAAAGACCGACCCGATCTTGGGAAGGTTATACTGCCTGAGCTTAACAGATTGAGAGCCCTCGCCGACGATAGCTTCCAATGTGTTATGTTTGGCGGAAGTGGAGTACTCTCACCAAATCAAGACTCAAATTCTACATCACAAGTGAGTTACATATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

743

Amino Acids

83.33

Weight (kDa)

7.05

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 17 - 123 1.1e-08 Universal stress protein family
Pkinase PF00069 443 - 695 9.1e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 444 - 700 6e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 1957
AccI GTMKAC 1 cut(s) 1368
AccII CGCG 2 cut(s) 242, 1084
AccIII TCCGGA 1 cut(s) 1417
AciI CCGC 4 cut(s) 461, 1787, 1957, 2166
AclWI GGATC 4 cut(s) 191, 726, 1832, 1988
AcoI YGGCCR 3 cut(s) 57, 166, 2012
AcsI RAATTY 6 cut(s) 322, 395, 863, 1037, 1322, 2200
AcuI CTGAAG 3 cut(s) 285, 540, 1254
AcyI GRCGYC 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 1895
AfaI GTAC 8 cut(s) 342, 700, 989, 1280, 1289, 1520, 1541, 2178
AfiI CCNNNNNNNGG 8 cut(s) 164, 359, 1453, 1520, 1660, 1934, 2090, 2091
AgeI ACCGGT 1 cut(s) 1044
AgsI TTSAA 7 cut(s) 327, 863, 1151, 1256, 1307, 1460, 2032
AjnI CCWGG 2 cut(s) 163, 1699
Alw21I GWGCWC 3 cut(s) 669, 969, 1686
Alw26I GTCTC 2 cut(s) 116, 1961
Alw44I GTGCAC 1 cut(s) 1682
AlwI GGATC 4 cut(s) 191, 726, 1832, 1988
AlwNI CAGNNNCTG 1 cut(s) 2006
Ama87I CYCGRG 2 cut(s) 275, 820
Aor13HI TCCGGA 1 cut(s) 1417
AoxI GGCC 5 cut(s) 57, 124, 166, 1361, 2012
ApaLI GTGCAC 1 cut(s) 1682
ApeKI GCWGC 8 cut(s) 1142, 1154, 1157, 1160, 1163, 1424, 1470, 1625
ApoI RAATTY 6 cut(s) 322, 395, 863, 1037, 1322, 2200
ArsI GACNNNNNNTTYG 4 cut(s) 644, 674, 676, 706
AsiGI ACCGGT 1 cut(s) 1044
AsiSI GCGATCGC 1 cut(s) 1439
Asp700I GAANNNNTTC 1 cut(s) 643
AspLEI GCGC 3 cut(s) 242, 1084, 1192
AspS9I GGNCC 2 cut(s) 124, 1361
AsuC2I CCSGG 1 cut(s) 1592
AsuHPI GGTGA 5 cut(s) 615, 861, 1388, 1568, 2175
AsuII TTCGAA 1 cut(s) 1618
AvaI CYCGRG 2 cut(s) 275, 820
BaeGI GKGCMC 2 cut(s) 18, 1686
BalI TGGCCA 2 cut(s) 59, 2014
BanII GRGCYC 3 cut(s) 669, 969, 2131
BbsI GAAGAC 3 cut(s) 646, 712, 1253
Bbv12I GWGCWC 3 cut(s) 669, 969, 1686
BbvCI CCTCAGC 1 cut(s) 1092
BbvI GCAGC 8 cut(s) 1144, 1154, 1166, 1172, 1175, 1436, 1457, 1612
BccI CCATC 4 cut(s) 510, 643, 880, 1780
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BciT130I CCWGG 2 cut(s) 165, 1701
BciVI GTATCC 1 cut(s) 738
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 1 cut(s) 1592
BcoDI GTCTC 2 cut(s) 116, 1961
BfaI CTAG 8 cut(s) 633, 962, 1088, 1331, 1451, 1715, 1758, 1892
BfmI CTRYAG 3 cut(s) 1158, 1626, 1998
BfuI GTATCC 1 cut(s) 738
BisI GCNGC 8 cut(s) 1143, 1155, 1158, 1161, 1164, 1425, 1471, 1626
BlpI GCTNAGC 1 cut(s) 1665
BlsI GCNGC 8 cut(s) 1144, 1156, 1159, 1162, 1165, 1426, 1472, 1627
BmcAI AGTACT 2 cut(s) 700, 2178
Bme1390I CCNGG 3 cut(s) 165, 1592, 1701
BmeT110I CYCGRG 2 cut(s) 275, 820
BmgT120I GGNCC 2 cut(s) 124, 1361
BmrFI CCNGG 3 cut(s) 165, 1592, 1701
BmsI GCATC 7 cut(s) 434, 578, 1393, 1411, 1774, 1890, 1977
BpiI GAAGAC 3 cut(s) 646, 712, 1253
BplI GAGNNNNNCTC 2 cut(s) 751, 783
Bpu10I CCTNAGC 3 cut(s) 956, 1092, 2109
Bpu1102I GCTNAGC 1 cut(s) 1665
Bpu14I TTCGAA 1 cut(s) 1618
BpuEI CTTGAG 3 cut(s) 953, 962, 1413
BpuMI CCSGG 1 cut(s) 1592
BsaAI YACGTR 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 120, 1579, 1590, 1601
BsaWI WCCGGW 2 cut(s) 1044, 1417
Bsc4I CCNNNNNNNGG 8 cut(s) 164, 359, 1453, 1520, 1660, 1934, 2090, 2091
Bse118I RCCGGY 1 cut(s) 1044
Bse1I ACTGG 5 cut(s) 706, 1188, 1364, 1394, 2015
Bse3DI GCAATG 2 cut(s) 137, 517
BseAI TCCGGA 1 cut(s) 1417
BseBI CCWGG 2 cut(s) 165, 1701
BseDI CCNNGG 4 cut(s) 120, 1579, 1590, 1601
BseGI GGATG 6 cut(s) 154, 502, 812, 872, 1426, 1480
BseLI CCNNNNNNNGG 8 cut(s) 164, 359, 1453, 1520, 1660, 1934, 2090, 2091
BseMI GCAATG 2 cut(s) 137, 517
BseMII CTCAG 5 cut(s) 798, 891, 970, 1083, 2100
BseNI ACTGG 5 cut(s) 706, 1188, 1364, 1394, 2015
BseRI GAGGAG 4 cut(s) 462, 964, 1213, 1313
BseSI GKGCMC 2 cut(s) 18, 1686
BseXI GCAGC 8 cut(s) 1144, 1154, 1166, 1172, 1175, 1436, 1457, 1612
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 2 cut(s) 242, 1084
Bsh1285I CGRYCG 1 cut(s) 1439
BshFI GGCC 5 cut(s) 59, 126, 168, 1363, 2014
BshTI ACCGGT 1 cut(s) 1044
BsiEI CGRYCG 1 cut(s) 1439
BsiHKAI GWGCWC 3 cut(s) 669, 969, 1686
BsiHKCI CYCGRG 2 cut(s) 275, 820
BsiSI CCGG 4 cut(s) 169, 1045, 1418, 1592
BslI CCNNNNNNNGG 8 cut(s) 164, 359, 1453, 1520, 1660, 1934, 2090, 2091
BsmAI GTCTC 2 cut(s) 116, 1961
BsmBI CGTCTC 1 cut(s) 1961
BsnI GGCC 5 cut(s) 59, 126, 168, 1363, 2014
BsoBI CYCGRG 2 cut(s) 275, 820
Bsp119I TTCGAA 1 cut(s) 1618
Bsp1286I GDGCHC 5 cut(s) 18, 669, 969, 1686, 2131
Bsp13I TCCGGA 1 cut(s) 1417
Bsp1407I TGTACA 1 cut(s) 987
Bsp143I GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
Bsp1720I GCTNAGC 1 cut(s) 1665
BspACI CCGC 4 cut(s) 461, 1787, 1957, 2166
BspANI GGCC 5 cut(s) 59, 126, 168, 1363, 2014
BspCNI CTCAG 5 cut(s) 797, 890, 969, 1084, 2101
BspEI TCCGGA 1 cut(s) 1417
BspFNI CGCG 2 cut(s) 242, 1084
BspMAI CTGCAG 3 cut(s) 1162, 1630, 2002
BspPI GGATC 4 cut(s) 191, 726, 1832, 1988
BspT104I TTCGAA 1 cut(s) 1618
BsrBI CCGCTC 1 cut(s) 1957
BsrDI GCAATG 2 cut(s) 137, 517
BsrFI RCCGGY 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 5 cut(s) 706, 1188, 1364, 1394, 2015
BssAI RCCGGY 1 cut(s) 1044
BssECI CCNNGG 4 cut(s) 120, 1579, 1590, 1601
BssMI GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
BssNAI GTATAC 1 cut(s) 1369
BssNI GRCGYC 1 cut(s) 389
BssT1I CCWWGG 2 cut(s) 120, 1601
Bst1107I GTATAC 1 cut(s) 1369
Bst2UI CCWGG 2 cut(s) 165, 1701
Bst4CI ACNGT 5 cut(s) 454, 1523, 1688, 1797, 2055
Bst6I CTCTTC 2 cut(s) 924, 1230
BstACI GRCGYC 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstAUI TGTACA 1 cut(s) 987
BstBAI YACGTR 1 cut(s) 479
BstBI TTCGAA 1 cut(s) 1618
BstC8I GCNNGC 7 cut(s) 602, 830, 1000, 1176, 1336, 1862, 1959
BstDEI CTNAG 7 cut(s) 784, 877, 956, 1092, 1610, 1665, 2109
BstENI CCTNNNNNAGG 1 cut(s) 1658
BstF5I GGATG 6 cut(s) 154, 502, 812, 872, 1426, 1480
BstFNI CGCG 2 cut(s) 242, 1084
BstHHI GCGC 3 cut(s) 242, 1084, 1192
BstKTI GATC 8 cut(s) 199, 306, 659, 721, 1439, 1840, 1996, 2089
BstMAI GTCTC 2 cut(s) 116, 1961
BstMBI GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
BstMCI CGRYCG 1 cut(s) 1439
BstMWI GCNNNNNNNGC 9 cut(s) 1016, 1088, 1097, 1106, 1151, 1160, 1163, 1447, 1634
BstNI CCWGG 2 cut(s) 165, 1701
BstNSI RCATGY 2 cut(s) 727, 1864
BstSCI CCNGG 3 cut(s) 163, 1590, 1699
BstSFI CTRYAG 3 cut(s) 1158, 1626, 1998
BstSLI GKGCMC 2 cut(s) 18, 1686
BstUI CGCG 2 cut(s) 242, 1084
BstV1I GCAGC 8 cut(s) 1144, 1154, 1166, 1172, 1175, 1436, 1457, 1612
BstV2I GAAGAC 3 cut(s) 646, 712, 1253
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BstZ17I GTATAC 1 cut(s) 1369
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 5 cut(s) 59, 126, 168, 1363, 2014
BtgZI GCGATG 2 cut(s) 400, 457
BtsCI GGATG 6 cut(s) 154, 502, 812, 872, 1426, 1480
BtsI GCAGTG 2 cut(s) 54, 60
BtsIMutI CAGTG 7 cut(s) 54, 60, 261, 299, 379, 1010, 2060
Cac8I GCNNGC 7 cut(s) 602, 830, 1000, 1176, 1336, 1862, 1959
CaiI CAGNNNCTG 1 cut(s) 2006
CfoI GCGC 3 cut(s) 242, 1084, 1192
Cfr10I RCCGGY 1 cut(s) 1044
Cfr13I GGNCC 2 cut(s) 124, 1361
CseI GACGC 3 cut(s) 29, 165, 397
Csp6I GTAC 8 cut(s) 341, 699, 988, 1279, 1288, 1519, 1540, 2177
CspAI ACCGGT 1 cut(s) 1044
CviAII CATG 7 cut(s) 146, 209, 724, 1489, 1543, 1861, 1949
CviQI GTAC 8 cut(s) 341, 699, 988, 1279, 1288, 1519, 1540, 2177
DdeI CTNAG 7 cut(s) 784, 877, 956, 1092, 1610, 1665, 2109
DpnI GATC 8 cut(s) 198, 305, 658, 720, 1438, 1839, 1995, 2088
DpnII GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
DraIII CACNNNGTG 1 cut(s) 1895
EaeI YGGCCR 3 cut(s) 57, 166, 2012
Eam1104I CTCTTC 2 cut(s) 924, 1230
EarI CTCTTC 2 cut(s) 924, 1230
EciI GGCGGA 1 cut(s) 2181
Ecl136II GAGCTC 2 cut(s) 667, 967
Eco130I CCWWGG 2 cut(s) 120, 1601
Eco24I GRGCYC 3 cut(s) 669, 969, 2131
Eco32I GATATC 1 cut(s) 1882
Eco53kI GAGCTC 2 cut(s) 667, 967
Eco57I CTGAAG 3 cut(s) 285, 540, 1254
Eco88I CYCGRG 2 cut(s) 275, 820
EcoICRI GAGCTC 2 cut(s) 667, 967
EcoNI CCTNNNNNAGG 1 cut(s) 1658
EcoRII CCWGG 2 cut(s) 163, 1699
EcoRV GATATC 1 cut(s) 1882
EcoT14I CCWWGG 2 cut(s) 120, 1601
EcoT38I GRGCYC 3 cut(s) 669, 969, 2131
ErhI CCWWGG 2 cut(s) 120, 1601
Esp3I CGTCTC 1 cut(s) 1961
FaeI CATG 7 cut(s) 149, 212, 727, 1492, 1546, 1864, 1952
FatI CATG 7 cut(s) 145, 208, 723, 1488, 1542, 1860, 1948
FauI CCCGC 1 cut(s) 1950
FauNDI CATATG 2 cut(s) 1267, 1803
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 1368
Fnu4HI GCNGC 8 cut(s) 1143, 1155, 1158, 1161, 1164, 1425, 1471, 1626
FokI GGATG 6 cut(s) 141, 489, 799, 859, 1433, 1467
FriOI GRGCYC 3 cut(s) 669, 969, 2131
Fsp4HI GCNGC 8 cut(s) 1143, 1155, 1158, 1161, 1164, 1425, 1471, 1626
FspBI CTAG 8 cut(s) 633, 962, 1088, 1331, 1451, 1715, 1758, 1892
GlaI GCGC 3 cut(s) 241, 1083, 1191
GluI GCNGC 8 cut(s) 1143, 1155, 1158, 1161, 1164, 1425, 1471, 1626
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 5 cut(s) 59, 126, 168, 1363, 2014
HapII CCGG 4 cut(s) 169, 1045, 1418, 1592
HgaI GACGC 3 cut(s) 29, 165, 397
HhaI GCGC 3 cut(s) 242, 1084, 1192
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 7 cut(s) 149, 212, 727, 1492, 1546, 1864, 1952
Hin6I GCGC 3 cut(s) 240, 1082, 1190
HinP1I GCGC 3 cut(s) 240, 1082, 1190
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 1975
HinfI GANTC 6 cut(s) 187, 530, 752, 1229, 1942, 2195
HpaII CCGG 4 cut(s) 169, 1045, 1418, 1592
HphI GGTGA 5 cut(s) 615, 861, 1388, 1568, 2175
Hpy166II GTNNAC 5 cut(s) 180, 1290, 1369, 1379, 1684
Hpy188I TCNGA 5 cut(s) 520, 574, 787, 1234, 1879
Hpy188III TCNNGA 6 cut(s) 768, 1418, 1514, 1841, 2006, 2192
Hpy8I GTNNAC 5 cut(s) 180, 1290, 1369, 1379, 1684
Hpy99I CGWCG 3 cut(s) 23, 774, 2141
HpyAV CCTTC 7 cut(s) 654, 741, 1055, 1343, 1508, 1664, 2089
HpyCH4III ACNGT 5 cut(s) 454, 1523, 1688, 1797, 2055
HpyCH4IV ACGT 3 cut(s) 478, 772, 1414
HpyF10VI GCNNNNNNNGC 9 cut(s) 1016, 1088, 1097, 1106, 1151, 1160, 1163, 1447, 1634
HpyF3I CTNAG 7 cut(s) 784, 877, 956, 1092, 1610, 1665, 2109
HpySE526I ACGT 3 cut(s) 478, 772, 1414
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 7 cut(s) 149, 212, 727, 1492, 1546, 1864, 1952
HspAI GCGC 3 cut(s) 240, 1082, 1190
Kpn2I TCCGGA 1 cut(s) 1417
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
LmnI GCTCC 6 cut(s) 147, 452, 672, 937, 1505, 1554
Lsp1109I GCAGC 8 cut(s) 1144, 1154, 1166, 1172, 1175, 1436, 1457, 1612
LweI GCATC 7 cut(s) 434, 578, 1393, 1411, 1774, 1890, 1977
MaeI CTAG 8 cut(s) 633, 962, 1088, 1331, 1451, 1715, 1758, 1892
MaeII ACGT 3 cut(s) 478, 772, 1414
MaeIII GTNAC 7 cut(s) 621, 680, 903, 1583, 1688, 1789, 2219
MalI GATC 8 cut(s) 198, 305, 658, 720, 1438, 1839, 1995, 2088
MbiI CCGCTC 1 cut(s) 1957
MboI GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
MboII GAAGA 7 cut(s) 651, 717, 941, 1070, 1247, 1255, 1258
MfeI CAATTG 2 cut(s) 327, 1293
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 5 cut(s) 18, 669, 969, 1686, 2131
MlsI TGGCCA 2 cut(s) 59, 2014
MluNI TGGCCA 2 cut(s) 59, 2014
MlyI GAGTC 2 cut(s) 1936, 2189
MmeI TCCRAC 2 cut(s) 377, 840
Mox20I TGGCCA 2 cut(s) 59, 2014
MroI TCCGGA 1 cut(s) 1417
MroXI GAANNNNTTC 1 cut(s) 643
MscI TGGCCA 2 cut(s) 59, 2014
MseI TTAA 7 cut(s) 35, 116, 315, 1041, 1224, 1694, 2115
Msp20I TGGCCA 2 cut(s) 59, 2014
MspA1I CMGCKG 2 cut(s) 1157, 1817
MspI CCGG 4 cut(s) 169, 1045, 1418, 1592
MspR9I CCNGG 3 cut(s) 165, 1592, 1701
MunI CAATTG 2 cut(s) 327, 1293
MvaI CCWGG 2 cut(s) 165, 1701
MvnI CGCG 2 cut(s) 242, 1084
MwoI GCNNNNNNNGC 9 cut(s) 1016, 1088, 1097, 1106, 1151, 1160, 1163, 1447, 1634
NciI CCSGG 1 cut(s) 1592
NdeI CATATG 2 cut(s) 1267, 1803
NdeII GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
NlaIII CATG 7 cut(s) 149, 212, 727, 1492, 1546, 1864, 1952
NmuCI GTSAC 5 cut(s) 621, 680, 903, 1688, 1789
NspI RCATGY 2 cut(s) 727, 1864
NspV TTCGAA 1 cut(s) 1618
PaeI GCATGC 1 cut(s) 1864
PaeR7I CTCGAG 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 643
PfeI GAWTC 4 cut(s) 187, 530, 752, 1229
PflFI GACNNNGTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 1044
PkrI GCNGC 8 cut(s) 1144, 1156, 1159, 1162, 1165, 1426, 1472, 1627
Ple19I CGATCG 1 cut(s) 1439
PleI GAGTC 2 cut(s) 1936, 2189
PpsI GAGTC 2 cut(s) 1936, 2189
Ppu21I YACGTR 1 cut(s) 479
Psp124BI GAGCTC 2 cut(s) 669, 969
Psp6I CCWGG 2 cut(s) 163, 1699
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 2 cut(s) 163, 1699
PspPI GGNCC 2 cut(s) 124, 1361
PspXI VCTCGAGB 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 333, 365
PstI CTGCAG 3 cut(s) 1162, 1630, 2002
PstNI CAGNNNCTG 1 cut(s) 2006
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 175
PvuI CGATCG 1 cut(s) 1439
PvuII CAGCTG 2 cut(s) 1157, 1817
RgaI GCGATCGC 1 cut(s) 1439
RsaI GTAC 8 cut(s) 342, 700, 989, 1280, 1289, 1520, 1541, 2178
RsaNI GTAC 8 cut(s) 341, 699, 988, 1279, 1288, 1519, 1540, 2177
SacI GAGCTC 2 cut(s) 669, 969
SaqAI TTAA 7 cut(s) 35, 116, 315, 1041, 1224, 1694, 2115
SatI GCNGC 8 cut(s) 1143, 1155, 1158, 1161, 1164, 1425, 1471, 1626
Sau3AI GATC 8 cut(s) 196, 303, 656, 718, 1436, 1837, 1993, 2086
Sau96I GGNCC 2 cut(s) 124, 1361
ScaI AGTACT 2 cut(s) 700, 2178
SchI GAGTC 2 cut(s) 1936, 2189
ScrFI CCNGG 3 cut(s) 165, 1592, 1701
SduI GDGCHC 5 cut(s) 18, 669, 969, 1686, 2131
SfaAI GCGATCGC 1 cut(s) 1439
SfaNI GCATC 7 cut(s) 434, 578, 1393, 1411, 1774, 1890, 1977
SfcI CTRYAG 3 cut(s) 1158, 1626, 1998
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 1618
SgfI GCGATCGC 1 cut(s) 1439
SlaI CTCGAG 1 cut(s) 275
SmlI CTYRAG 4 cut(s) 275, 941, 968, 1428
SmoI CTYRAG 4 cut(s) 275, 941, 968, 1428
SphI GCATGC 1 cut(s) 1864
SsiI CCGC 4 cut(s) 461, 1787, 1957, 2166
SspMI CTAG 8 cut(s) 633, 962, 1088, 1331, 1451, 1715, 1758, 1892
SstI GAGCTC 2 cut(s) 669, 969
StyD4I CCNGG 3 cut(s) 163, 1590, 1699
StyI CCWWGG 2 cut(s) 120, 1601
TaaI ACNGT 5 cut(s) 454, 1523, 1688, 1797, 2055
TaiI ACGT 3 cut(s) 481, 775, 1417
TaqI TCGA 4 cut(s) 276, 647, 769, 1618
TaqII GACCGA 1 cut(s) 2094
TatI WGTACW 5 cut(s) 698, 987, 1287, 1539, 2176
TfiI GAWTC 4 cut(s) 187, 530, 752, 1229
Tru1I TTAA 7 cut(s) 35, 116, 315, 1041, 1224, 1694, 2115
Tru9I TTAA 7 cut(s) 35, 116, 315, 1041, 1224, 1694, 2115
TscAI CASTG 7 cut(s) 54, 60, 268, 306, 379, 1017, 2060
TseFI GTSAC 5 cut(s) 621, 680, 903, 1688, 1789
TseI GCWGC 8 cut(s) 1142, 1154, 1157, 1160, 1163, 1424, 1470, 1625
Tsp45I GTSAC 5 cut(s) 621, 680, 903, 1688, 1789
TspDTI ATGAA 5 cut(s) 529, 627, 1050, 1229, 1646
TspGWI ACGGA 2 cut(s) 197, 806
TspRI CASTG 7 cut(s) 54, 60, 268, 306, 379, 1017, 2060
Tth111I GACNNNGTC 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 164
VneI GTGCAC 1 cut(s) 1682
XagI CCTNNNNNAGG 1 cut(s) 1658
XapI RAATTY 6 cut(s) 322, 395, 863, 1037, 1322, 2200
XceI RCATGY 2 cut(s) 727, 1864
XcmI CCANNNNNNNNNTGG 1 cut(s) 1930
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 1368
XmnI GAANNNNTTC 1 cut(s) 643
XspI CTAG 8 cut(s) 633, 962, 1088, 1331, 1451, 1715, 1758, 1892
ZrmI AGTACT 2 cut(s) 700, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.