RchiOBHm_Chr1g0332091

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000353
Physical Location & Seq
Forward (+)
23220052 .. 23224549
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56103

Sequence Viewer

Length: 2346 bp
ATGGCAAGATCATGTGCCACAAGTGATGAAAAGAGAGAAGAGGTGGTGGCGGTGGCAATAGACAAGGACAAGGGAAGTCAATACGCTCTCAAATGGACTGTCGAGCATCTTCTTGTCAAGGGCCAAAGTCTCACCTTTGTGCATGTTAAACAATCAACACCAGGCCTTCCCAATCAAACTGAGAAATCGGTTTCTGGCTCTGAATTAAAGGAGGAAGGTATAAAATTGTGCAAAAGCCAATCTGATGCTGAATATAGCGAGTTGTTTCTTCCACTTCGTACTTTTTGTAAGAAAAAGCTTGTAAGATACAACGAAGTAATAGTTAAAGGTACTGATGTAGCAAAAGCACTTGTCAATTTTATTTCGACACATTCAATTGAGGTTTTGGTACTTGCGGCACCTTCAAAAAGTGGCTTTTTCAGAGCCTTCAACAAAACTACAGACATTCCAAGCAGAGTGTCGAAAGGGGCACCGGATTTCTGCACTGTGTATGTCATTGGCAAGGGAAAGATCAGTCATGTGCAAACAGCAACCACTAAATTACCCCCAGAAGCTCGTAATAAGCAAATACATAACCAACTCAGCAATGTTTCGGACTCAAGTGTTTCTCAATTCATGCGTAACCAGCAACCCAGAGGTTATATCACTTTTGTAATTCTACCATCATGCATGTTGACACCATTCACCAGAGGACAAGCTTCATCAAACAGATTATATGAGATCTCACCAGACAGCGATGTATCATTCACAAGTAGTGGAGGAAGACCAAGCATTGATCACATGTTTCCTTCTATATGCGGCAGTGTGGAATCAGGAACGCACTCTCAACAATCATTCAACTCAGACTTAGACAACAGAAGATCTACCTCCAAGCAATCCTACTCAAGAACCCAGTTCATTGACATGAACTCCTCATTATCCTCAACCGAGAGTGGAAAATCATGGTCATCCCGAAATATGGAAGAAGTAGAAGATGAGATGATGAGACTCAAGCTAGAGCTCAAGCAGACAATGGAGGTGTACAATACGGCCTGTAAGGAAACCCTAACATCAAAACAGAAGCAACTTGACCAATGGAAATGGGAAGAAGAACAAAGATTGGAAGGGGCACACCTAGGTCAGGAGTTTGCATTGGCACTTGTACAGAAAGAGGAAGCAAAATGTAGGGCAGCAATGGACGCAGAAAGAGCAGCTAAAAGGATTGCTGAATTGGAAGCAGAACAAAGGAGGAATTCAGAAATAAAGGGTCTGAAAGAAGCTGAAAAGAGGACGAACTCACTAGGAGGAAGAAGTTTTGATCTGCGGTACCGGAAATACACAATTGAAGAGATTAAAGCAGCAACGAACAGTTTTTCAAAAGCTCTTAAGATTGGAGAAGGTGGCTATGGGCCTGTGTATGTGGGTGAGCTGGACCATACACAAGTGGCAATAAAAGCTCTACGTCCAGATGCAGCTCAAGGACGTTCACAGTTCCAACAAGAGATTGAAGTTTTGAGCTGCATAAGACATCCAAACATGGTTCTCCTGCTTGGAGCCTGCCCAGAGTATGGTTGTCTGATCTATGAGTACATGGGTAATGGGAGCTTAGAAGACCGTCTGTTTCGACGAGGTAACACCCCAGTTATTCCTTGGCAGCTAAGGTTCCGAATCGCTGCAGAAATTGCCACGGGGCTTTTGTTCCTTCACCAGGCCAAGCCAGAACCCTTTGTACACCGTGACTTAAAGCCAGGAAACATTTTGCTAGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTCTGGCCAGGCTTGTCCCTCCATCAGTAGCTGACTCAGTCACTCAGTATCACATGACATCAGCAGCTGGGACATTCTGTTATATCGACCCAGAGTATCAACAAACTGGCTTGCTTGGAACAAAATCTGATATCTACTCACTTGGGGTAATGCTTCTACAAATAATAACAGCCAAACCAGCAATGGGTTTGACTCATCTTGTTGAGCACGCAATTGAGATAGGGACCTTCGCTGAGATGCTTGACCCCGCTGTTCCTGACTGGCCTGTGGAGGAAGCCTTGAAGTTTGCCAAGTTAGCATTACAATGTGCCGAAATGAGGCGAAAAGACAGACCCGATCTTGGCAAGGTAGTGCTACCTGAGCTTAACAGATTGAGAGAACTCGGCGAAAAGGGTGGCATGCTTAATGGTGGTGGAGCATTCTTACTACAAAAGTGCTCAGTTTCCACAAAGCAAGAAGTGATGAATGACCCCCAATCTGGATATGCCAGTTCAGTGAGCAATTCGAGCACATCATCTTATGGAGGAAAGAATATTATAGTCGGATTCTTGACGTTTTCTTCGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

781

Amino Acids

86.66

Weight (kDa)

8.34

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 453 - 704 3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 456 - 709 4.3e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1305
AccB1I GGYRCC 3 cut(s) 397, 469, 1305
AccB7I CCANNNNNTGG 1 cut(s) 1547
AciI CCGC 5 cut(s) 50, 395, 798, 1303, 2025
AcoI YGGCCR 1 cut(s) 1782
AcsI RAATTY 1 cut(s) 1231
AfaI GTAC 8 cut(s) 280, 331, 390, 1022, 1143, 1307, 1568, 1710
AfiI CCNNNNNNNGG 8 cut(s) 958, 1120, 1547, 1687, 1961, 2094, 2117, 2255
AflII CTTAAG 1 cut(s) 1364
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 8 cut(s) 375, 405, 430, 838, 1325, 1356, 1487, 2059
AjnI CCWGG 4 cut(s) 160, 1686, 1726, 1784
AleI CACNNNNGTG 1 cut(s) 137
Alw21I GWGCWC 4 cut(s) 1002, 1986, 2216, 2288
Alw26I GTCTC 2 cut(s) 134, 979
AlwNI CAGNNNCTG 2 cut(s) 1808, 1844
AoxI GGCC 7 cut(s) 121, 163, 1029, 1388, 1689, 1782, 2039
ApeKI GCWGC 8 cut(s) 1169, 1190, 1337, 1451, 1497, 1633, 1652, 1841
ApoI RAATTY 1 cut(s) 1231
ArsI GACNNNNNNTTYG 3 cut(s) 84, 116, 2320
Asp718I GGTACC 1 cut(s) 1305
AspA2I CCTAGG 1 cut(s) 1114
AspS9I GGNCC 4 cut(s) 121, 1388, 1411, 2001
AsuHPI GGTGA 5 cut(s) 124, 676, 717, 1415, 1676
AvaII GGWCC 2 cut(s) 1411, 2001
AvrII CCTAGG 1 cut(s) 1114
BaeGI GKGCMC 2 cut(s) 472, 1111
BaeI ACNNNNGTAYC 2 cut(s) 321, 354
BalI TGGCCA 1 cut(s) 1784
BanI GGYRCC 3 cut(s) 397, 469, 1305
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 2 cut(s) 769, 1596
Bbv12I GWGCWC 4 cut(s) 1002, 1986, 2216, 2288
BbvI GCAGC 8 cut(s) 1181, 1202, 1349, 1463, 1484, 1639, 1645, 1853
BccI CCATC 2 cut(s) 670, 1807
BceAI ACGGC 1 cut(s) 1044
BciT130I CCWGG 4 cut(s) 162, 1688, 1728, 1786
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 2 cut(s) 134, 979
BfaI CTAG 4 cut(s) 995, 1115, 1280, 1742
BfmI CTRYAG 2 cut(s) 438, 1653
BfrI CTTAAG 1 cut(s) 1364
BglII AGATCT 2 cut(s) 720, 860
BlnI CCTAGG 1 cut(s) 1114
Bme1390I CCNGG 4 cut(s) 162, 1688, 1728, 1786
Bme18I GGWCC 2 cut(s) 1411, 2001
BmgT120I GGNCC 4 cut(s) 121, 1388, 1411, 2001
BmiI GGNNCC 6 cut(s) 399, 471, 1307, 1534, 1643, 2002
BmrFI CCNGG 4 cut(s) 162, 1688, 1728, 1786
BmrI ACTGGG 2 cut(s) 886, 1613
BmsI GCATC 4 cut(s) 115, 235, 1438, 2004
BmuI ACTGGG 2 cut(s) 886, 1613
BpiI GAAGAC 2 cut(s) 769, 1596
Bpu10I CCTNAGC 2 cut(s) 1637, 2136
BpuEI CTTGAG 5 cut(s) 583, 868, 974, 986, 1440
BsaJI CCNNGG 3 cut(s) 1114, 1628, 1665
BsaWI WCCGGW 2 cut(s) 472, 1308
BsaXI ACNNNNNCTCC 2 cut(s) 893, 923
Bsc4I CCNNNNNNNGG 8 cut(s) 958, 1120, 1547, 1687, 1961, 2094, 2117, 2255
Bse1I ACTGG 5 cut(s) 892, 1619, 1888, 2042, 2265
Bse3DI GCAATG 3 cut(s) 592, 1179, 1965
BseBI CCWGG 4 cut(s) 162, 1688, 1728, 1786
BseDI CCNNGG 3 cut(s) 1114, 1628, 1665
BseGI GGATG 2 cut(s) 947, 1507
BseLI CCNNNNNNNGG 8 cut(s) 958, 1120, 1547, 1687, 1961, 2094, 2117, 2255
BseMI GCAATG 3 cut(s) 592, 1179, 1965
BseMII CTCAG 8 cut(s) 171, 595, 855, 1827, 1835, 2001, 2127, 2229
BseNI ACTGG 5 cut(s) 892, 1619, 1888, 2042, 2265
BseRI GAGGAG 1 cut(s) 901
BseSI GKGCMC 2 cut(s) 472, 1111
BseXI GCAGC 8 cut(s) 1181, 1202, 1349, 1463, 1484, 1639, 1645, 1853
BseYI CCCAGC 1 cut(s) 1844
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 7 cut(s) 123, 165, 1031, 1390, 1691, 1784, 2041
BshNI GGYRCC 3 cut(s) 397, 469, 1305
BsiHKAI GWGCWC 4 cut(s) 1002, 1986, 2216, 2288
BsiSI CCGG 2 cut(s) 473, 1309
BslFI GGGAC 3 cut(s) 1778, 1861, 2014
BslI CCNNNNNNNGG 8 cut(s) 958, 1120, 1547, 1687, 1961, 2094, 2117, 2255
BsmAI GTCTC 2 cut(s) 134, 979
BsmFI GGGAC 3 cut(s) 1778, 1861, 2014
BsmI GAATGC 1 cut(s) 2195
BsnI GGCC 7 cut(s) 123, 165, 1031, 1390, 1691, 1784, 2041
Bsp1286I GDGCHC 6 cut(s) 472, 1002, 1111, 1986, 2216, 2288
Bsp1407I TGTACA 3 cut(s) 1020, 1141, 1708
Bsp143I GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
BspACI CCGC 5 cut(s) 50, 395, 798, 1303, 2025
BspANI GGCC 7 cut(s) 123, 165, 1031, 1390, 1691, 1784, 2041
BspCNI CTCAG 8 cut(s) 172, 594, 854, 1826, 1834, 2002, 2128, 2228
BspLI GGNNCC 6 cut(s) 399, 471, 1307, 1534, 1643, 2002
BspMAI CTGCAG 1 cut(s) 1657
BspT107I GGYRCC 3 cut(s) 397, 469, 1305
BspTI CTTAAG 1 cut(s) 1364
BsrDI GCAATG 3 cut(s) 592, 1179, 1965
BsrGI TGTACA 3 cut(s) 1020, 1141, 1708
BsrI ACTGG 5 cut(s) 892, 1619, 1888, 2042, 2265
BssECI CCNNGG 3 cut(s) 1114, 1628, 1665
BssMI GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
BssT1I CCWWGG 2 cut(s) 1114, 1628
Bst2UI CCWGG 4 cut(s) 162, 1688, 1728, 1786
Bst4CI ACNGT 6 cut(s) 100, 487, 1349, 1470, 1595, 1715
Bst6I CTCTTC 2 cut(s) 33, 1320
BstAFI CTTAAG 1 cut(s) 1364
BstAPI GCANNNNNTGC 1 cut(s) 1661
BstAUI TGTACA 3 cut(s) 1020, 1141, 1708
BstC8I GCNNGC 4 cut(s) 1537, 1889, 1986, 2177
BstDSI CCRYGG 1 cut(s) 1665
BstENI CCTNNNNNAGG 2 cut(s) 1118, 1685
BstF5I GGATG 2 cut(s) 947, 1507
BstKTI GATC 8 cut(s) 11, 513, 723, 778, 863, 1300, 1560, 2116
BstMAI GTCTC 2 cut(s) 134, 979
BstMBI GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
BstMWI GCNNNNNNNGC 9 cut(s) 625, 1178, 1187, 1433, 1661, 1955, 2072, 2137, 2283
BstNI CCWGG 4 cut(s) 162, 1688, 1728, 1786
BstNSI RCATGY 4 cut(s) 146, 673, 784, 2179
BstSCI CCNGG 4 cut(s) 160, 1686, 1726, 1784
BstSFI CTRYAG 2 cut(s) 438, 1653
BstSLI GKGCMC 2 cut(s) 472, 1111
BstV1I GCAGC 8 cut(s) 1181, 1202, 1349, 1463, 1484, 1639, 1645, 1853
BstV2I GAAGAC 2 cut(s) 769, 1596
BstX2I RGATCY 2 cut(s) 720, 860
BstYI RGATCY 2 cut(s) 720, 860
BsuRI GGCC 7 cut(s) 123, 165, 1031, 1390, 1691, 1784, 2041
BtgI CCRYGG 1 cut(s) 1665
BtgZI GCGATG 1 cut(s) 750
BtsCI GGATG 2 cut(s) 947, 1507
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 3 cut(s) 483, 808, 2277
Cac8I GCNNGC 4 cut(s) 1537, 1889, 1986, 2177
CaiI CAGNNNCTG 2 cut(s) 1808, 1844
Cfr13I GGNCC 4 cut(s) 121, 1388, 1411, 2001
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 8 cut(s) 279, 330, 389, 1021, 1142, 1306, 1567, 1709
CviQI GTAC 8 cut(s) 279, 330, 389, 1021, 1142, 1306, 1567, 1709
DpnI GATC 8 cut(s) 10, 512, 722, 777, 862, 1299, 1559, 2115
DpnII GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
EaeI YGGCCR 1 cut(s) 1782
Eam1104I CTCTTC 2 cut(s) 33, 1320
EarI CTCTTC 2 cut(s) 33, 1320
Ecl136II GAGCTC 1 cut(s) 1000
Eco130I CCWWGG 2 cut(s) 1114, 1628
Eco147I AGGCCT 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 1002
Eco32I GATATC 1 cut(s) 1909
Eco47I GGWCC 2 cut(s) 1411, 2001
Eco53kI GAGCTC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 1000
EcoNI CCTNNNNNAGG 2 cut(s) 1118, 1685
EcoO109I RGGNCCY 1 cut(s) 2001
EcoRI GAATTC 1 cut(s) 1231
EcoRII CCWGG 4 cut(s) 160, 1686, 1726, 1784
EcoRV GATATC 1 cut(s) 1909
EcoT14I CCWWGG 2 cut(s) 1114, 1628
EcoT22I ATGCAT 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 2 cut(s) 1114, 1628
FaqI GGGAC 3 cut(s) 1778, 1861, 2014
FauI CCCGC 1 cut(s) 2032
FbaI TGATCA 1 cut(s) 775
FokI GGATG 2 cut(s) 934, 1494
FriOI GRGCYC 1 cut(s) 1002
FspBI CTAG 4 cut(s) 995, 1115, 1280, 1742
GsaI CCCAGC 1 cut(s) 1848
HaeIII GGCC 7 cut(s) 123, 165, 1031, 1390, 1691, 1784, 2041
HapII CCGG 2 cut(s) 473, 1309
HgaI GACGC 1 cut(s) 1187
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HindIII AAGCTT 2 cut(s) 296, 696
HinfI GANTC 7 cut(s) 596, 809, 987, 1647, 1811, 1969, 2322
HpaII CCGG 2 cut(s) 473, 1309
HphI GGTGA 5 cut(s) 124, 676, 717, 1415, 1676
Hpy166II GTNNAC 4 cut(s) 675, 1021, 1466, 1711
Hpy188III TCNNGA 8 cut(s) 813, 885, 951, 1121, 1445, 2033, 2256, 2326
Hpy8I GTNNAC 4 cut(s) 675, 1021, 1466, 1711
Hpy99I CGWCG 1 cut(s) 1608
HpyAV CCTTC 9 cut(s) 176, 209, 411, 436, 798, 1097, 1370, 1691, 2014
HpyCH4III ACNGT 6 cut(s) 100, 487, 1349, 1470, 1595, 1715
HpyCH4IV ACGT 3 cut(s) 1441, 1462, 2330
HpyCH4V TGCA 9 cut(s) 142, 231, 483, 523, 669, 1130, 1451, 1500, 1655
HpyF10VI GCNNNNNNNGC 9 cut(s) 625, 1178, 1187, 1433, 1661, 1955, 2072, 2137, 2283
HpySE526I ACGT 3 cut(s) 1441, 1462, 2330
KpnI GGTACC 1 cut(s) 1309
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
LmnI GCTCC 3 cut(s) 1532, 1581, 2192
Lsp1109I GCAGC 8 cut(s) 1181, 1202, 1349, 1463, 1484, 1639, 1645, 1853
LweI GCATC 4 cut(s) 115, 235, 1438, 2004
MaeI CTAG 4 cut(s) 995, 1115, 1280, 1742
MaeII ACGT 3 cut(s) 1441, 1462, 2330
MaeIII GTNAC 4 cut(s) 620, 1610, 1715, 1816
MalI GATC 8 cut(s) 10, 512, 722, 777, 862, 1299, 1559, 2115
MboI GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
MfeI CAATTG 3 cut(s) 375, 1320, 1989
MflI RGATCY 2 cut(s) 720, 860
MhlI GDGCHC 6 cut(s) 472, 1002, 1111, 1986, 2216, 2288
MlsI TGGCCA 1 cut(s) 1784
MluNI TGGCCA 1 cut(s) 1784
MlyI GAGTC 4 cut(s) 590, 981, 1805, 1963
MmeI TCCRAC 2 cut(s) 1498, 2299
Mox20I TGGCCA 1 cut(s) 1784
Mph1103I ATGCAT 1 cut(s) 671
MscI TGGCCA 1 cut(s) 1784
MseI TTAA 8 cut(s) 147, 206, 324, 1332, 1365, 1721, 2142, 2181
MslI CAYNNNNRTG 4 cut(s) 137, 902, 1752, 2080
Msp20I TGGCCA 1 cut(s) 1784
MspA1I CMGCKG 2 cut(s) 1844, 2027
MspCI CTTAAG 1 cut(s) 1364
MspI CCGG 2 cut(s) 473, 1309
MspR9I CCNGG 4 cut(s) 162, 1688, 1728, 1786
MunI CAATTG 3 cut(s) 375, 1320, 1989
Mva1269I GAATGC 1 cut(s) 2195
MvaI CCWGG 4 cut(s) 162, 1688, 1728, 1786
MwoI GCNNNNNNNGC 9 cut(s) 625, 1178, 1187, 1433, 1661, 1955, 2072, 2137, 2283
NdeII GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
NlaIV GGNNCC 6 cut(s) 399, 471, 1307, 1534, 1643, 2002
NmeAIII GCCGAG 1 cut(s) 2139
NmuCI GTSAC 2 cut(s) 1715, 1816
NsiI ATGCAT 1 cut(s) 671
NspI RCATGY 4 cut(s) 146, 673, 784, 2179
OliI CACNNNNGTG 1 cut(s) 137
PaeI GCATGC 1 cut(s) 2179
PceI AGGCCT 1 cut(s) 165
PciI ACATGT 1 cut(s) 780
PctI GAATGC 1 cut(s) 2195
PfeI GAWTC 3 cut(s) 809, 1647, 2322
PflFI GACNNNGTC 1 cut(s) 1814
PflMI CCANNNNNTGG 1 cut(s) 1547
PleI GAGTC 4 cut(s) 590, 981, 1805, 1963
PpsI GAGTC 4 cut(s) 590, 981, 1805, 1963
PpuMI RGGWCCY 1 cut(s) 2001
PscI ACATGT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 2001
Psp6I CCWGG 4 cut(s) 160, 1686, 1726, 1784
PspFI CCCAGC 1 cut(s) 1844
PspGI CCWGG 4 cut(s) 160, 1686, 1726, 1784
PspN4I GGNNCC 6 cut(s) 399, 471, 1307, 1534, 1643, 2002
PspPI GGNCC 4 cut(s) 121, 1388, 1411, 2001
PspPPI RGGWCCY 1 cut(s) 2001
PsrI GAACNNNNNNTAC 2 cut(s) 1692, 1724
PstI CTGCAG 1 cut(s) 1657
PstNI CAGNNNCTG 2 cut(s) 1808, 1844
PsuI RGATCY 2 cut(s) 720, 860
PsyI GACNNNGTC 1 cut(s) 1814
PvuII CAGCTG 1 cut(s) 1844
RsaI GTAC 8 cut(s) 280, 331, 390, 1022, 1143, 1307, 1568, 1710
RsaNI GTAC 8 cut(s) 279, 330, 389, 1021, 1142, 1306, 1567, 1709
RseI CAYNNNNRTG 4 cut(s) 137, 902, 1752, 2080
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 8 cut(s) 147, 206, 324, 1332, 1365, 1721, 2142, 2181
Sau3AI GATC 8 cut(s) 8, 510, 720, 775, 860, 1297, 1557, 2113
Sau96I GGNCC 4 cut(s) 121, 1388, 1411, 2001
SchI GAGTC 4 cut(s) 590, 981, 1805, 1963
ScrFI CCNGG 4 cut(s) 162, 1688, 1728, 1786
SduI GDGCHC 6 cut(s) 472, 1002, 1111, 1986, 2216, 2288
SfaNI GCATC 4 cut(s) 115, 235, 1438, 2004
SfcI CTRYAG 2 cut(s) 438, 1653
SinI GGWCC 2 cut(s) 1411, 2001
SmiMI CAYNNNNRTG 4 cut(s) 137, 902, 1752, 2080
SmlI CTYRAG 6 cut(s) 598, 883, 989, 1001, 1364, 1455
SmoI CTYRAG 6 cut(s) 598, 883, 989, 1001, 1364, 1455
SphI GCATGC 1 cut(s) 2179
SseBI AGGCCT 1 cut(s) 165
SsiI CCGC 5 cut(s) 50, 395, 798, 1303, 2025
SspI AATATT 1 cut(s) 2311
SspMI CTAG 4 cut(s) 995, 1115, 1280, 1742
SstI GAGCTC 1 cut(s) 1002
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 4 cut(s) 160, 1686, 1726, 1784
StyI CCWWGG 2 cut(s) 1114, 1628
TaaI ACNGT 6 cut(s) 100, 487, 1349, 1470, 1595, 1715
TaiI ACGT 3 cut(s) 1444, 1465, 2333
TaqI TCGA 6 cut(s) 102, 365, 461, 1603, 1863, 2282
TatI WGTACW 4 cut(s) 1020, 1141, 1566, 1708
TauI GCSGC 2 cut(s) 398, 801
TfiI GAWTC 3 cut(s) 809, 1647, 2322
Tru1I TTAA 8 cut(s) 147, 206, 324, 1332, 1365, 1721, 2142, 2181
Tru9I TTAA 8 cut(s) 147, 206, 324, 1332, 1365, 1721, 2142, 2181
TscAI CASTG 3 cut(s) 490, 808, 2277
TseFI GTSAC 2 cut(s) 1715, 1816
TseI GCWGC 8 cut(s) 1169, 1190, 1337, 1451, 1497, 1633, 1652, 1841
Tsp45I GTSAC 2 cut(s) 1715, 1816
TspDTI ATGAA 6 cut(s) 42, 604, 690, 886, 920, 2255
TspRI CASTG 3 cut(s) 490, 808, 2277
Tth111I GACNNNGTC 1 cut(s) 1814
Van91I CCANNNNNTGG 1 cut(s) 1547
Vha464I CTTAAG 1 cut(s) 1364
VpaK11BI GGWCC 2 cut(s) 1411, 2001
XagI CCTNNNNNAGG 2 cut(s) 1118, 1685
XapI RAATTY 1 cut(s) 1231
XceI RCATGY 4 cut(s) 146, 673, 784, 2179
XcmI CCANNNNNNNNNTGG 2 cut(s) 1626, 1957
XmaJI CCTAGG 1 cut(s) 1114
XspI CTAG 4 cut(s) 995, 1115, 1280, 1742
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.