Rh2DG396700

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000353
Physical Location & Seq
Forward (+)
57729910 .. 57737774
7865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG396700.1

Sequence Viewer

Length: 534 bp
ATGGGACTAAGAAGGCAAGCATCCAGAAACAGAGGAGATGCAGTAGCAGTGGCAATAGACAAGGACAAAGGAAGTCAATATGCTTTCCAATGGGTTGTTAATCATCTCGTCAAGCCAGGCCAAACTCTCACATTGCTTCATGTTAAGCAACCTGCAGTTCCCAACCAAAGTTCTTTGATGAATGCACCATCAGAAAGGACACAGTCTCCGCAGTGTAGCCTGCCAAATGACACTAAACTCGAGTCAGCAACTATATTACCGGTGAGCAGCAACAAGCAAAGCTTGGGTCGCACCTTTGCTTTCTCTGATAGTATGACAGCAGGAATGCCCCCTAGGCATCCAACTAGCTCAGAGTCAGACAGAAGCTCTACATCGACATCTTACTCCGGAAGGACATCTTGCTCCGGAACCGAGTTTATTGACATGACCTATCCACAGAATGAATTTTCATCTTCCTCAACAGAAAGTGGAAGGTTATCAACGTCACGAACCTACAAGTTTCAAGGCATCCATCCAACTGTTAACATGTCATAA

Protein Analysis

177

Amino Acids

19.25

Weight (kDa)

9.46

Isoelectric Point (pI)

67.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 15 - 70 6e-06 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccIII TCCGGA 2 cut(s) 386, 404
AciI CCGC 1 cut(s) 209
AcsI RAATTY 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 525
AgeI ACCGGT 1 cut(s) 259
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 3 cut(s) 282, 348, 366
AluI AGCT 3 cut(s) 282, 348, 366
Alw26I GTCTC 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 239
Aor13HI TCCGGA 2 cut(s) 386, 404
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 1 cut(s) 267
ApoI RAATTY 1 cut(s) 443
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
AsiGI ACCGGT 1 cut(s) 259
AspA2I CCTAGG 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 239
AvrII CCTAGG 1 cut(s) 332
BbvI GCAGC 1 cut(s) 279
BccI CCATC 2 cut(s) 196, 519
BciT130I CCWGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 333, 345
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 334
BisI GCNGC 1 cut(s) 268
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 1 cut(s) 269
Bme1390I CCNGG 1 cut(s) 117
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 4 cut(s) 28, 29, 346, 516
BsaJI CCNNGG 1 cut(s) 332
BsaWI WCCGGW 3 cut(s) 259, 386, 404
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 190, 220
Bse118I RCCGGY 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 131
BseAI TCCGGA 2 cut(s) 386, 404
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 332
BseGI GGATG 4 cut(s) 20, 337, 507, 511
BseMI GCAATG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 279
BshFI GGCC 1 cut(s) 120
BshTI ACCGGT 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 3 cut(s) 260, 387, 405
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 187, 330
BsnI GGCC 1 cut(s) 120
BsoBI CYCGRG 1 cut(s) 239
Bsp13I TCCGGA 2 cut(s) 386, 404
BspACI CCGC 1 cut(s) 209
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 362
BspEI TCCGGA 2 cut(s) 386, 404
BspLI GGNNCC 1 cut(s) 409
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 204, 520
BstC8I GCNNGC 2 cut(s) 18, 221
BstDEI CTNAG 2 cut(s) 8, 349
BstF5I GGATG 4 cut(s) 20, 337, 507, 511
BstMAI GTCTC 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 288, 334
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 279
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 4 cut(s) 20, 337, 507, 511
BtsI GCAGTG 2 cut(s) 54, 218
BtsIMutI CAGTG 2 cut(s) 54, 218
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 2 cut(s) 18, 221
Cfr10I RCCGGY 1 cut(s) 259
CspAI ACCGGT 1 cut(s) 259
CviAII CATG 3 cut(s) 140, 424, 526
CviJI RGCY 6 cut(s) 115, 120, 219, 282, 348, 366
CviKI_1 RGCY 6 cut(s) 115, 120, 219, 282, 348, 366
DdeI CTNAG 2 cut(s) 8, 349
Eco130I CCWWGG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 332
FaeI CATG 3 cut(s) 143, 427, 529
FaiI YATR 7 cut(s) 81, 141, 254, 314, 425, 527, 532
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 382, 414
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 139, 423, 525
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 4 cut(s) 7, 324, 494, 498
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 2 cut(s) 333, 345
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 1 cut(s) 120
HapII CCGG 3 cut(s) 260, 387, 405
Hin1II CATG 3 cut(s) 143, 427, 529
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 2 cut(s) 242, 353
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 3 cut(s) 260, 387, 405
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 4 cut(s) 193, 307, 352, 358
Hpy188III TCNNGA 4 cut(s) 24, 387, 405, 486
Hpy8I GTNNAC 1 cut(s) 523
HpyAV CCTTC 3 cut(s) 6, 384, 465
HpyCH4III ACNGT 2 cut(s) 204, 520
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 3 cut(s) 41, 155, 185
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 334
HpyF3I CTNAG 2 cut(s) 8, 349
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 3 cut(s) 143, 427, 529
Kpn2I TCCGGA 2 cut(s) 386, 404
KspAI GTTAAC 1 cut(s) 523
LmnI GCTCC 1 cut(s) 407
LpnPI CCDG 9 cut(s) 37, 102, 129, 165, 233, 273, 306, 400, 418
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 4 cut(s) 28, 29, 346, 516
MaeI CTAG 2 cut(s) 333, 345
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 1 cut(s) 483
MboII GAAGA 1 cut(s) 444
MluCI AATT 1 cut(s) 443
MlyI GAGTC 2 cut(s) 251, 362
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 2 cut(s) 26, 466
MroI TCCGGA 2 cut(s) 386, 404
MseI TTAA 3 cut(s) 99, 144, 522
MspI CCGG 3 cut(s) 260, 387, 405
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 2 cut(s) 187, 330
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 288, 334
NlaIII CATG 3 cut(s) 143, 427, 529
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 483
NspI RCATGY 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 239
PciI ACATGT 1 cut(s) 525
PctI GAATGC 2 cut(s) 187, 330
PflFI GACNNNGTC 1 cut(s) 202
PinAI ACCGGT 1 cut(s) 259
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 2 cut(s) 250, 361
PpsI GAGTC 2 cut(s) 250, 361
PscI ACATGT 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 409
PspXI VCTCGAGB 1 cut(s) 239
PstI CTGCAG 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 202
SaqAI TTAA 3 cut(s) 99, 144, 522
SatI GCNGC 1 cut(s) 268
SchI GAGTC 2 cut(s) 251, 362
ScrFI CCNGG 1 cut(s) 117
SetI ASST 9 cut(s) 154, 284, 296, 350, 368, 431, 476, 485, 494
SfaNI GCATC 4 cut(s) 28, 29, 346, 516
SfcI CTRYAG 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 239
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 443
SsiI CCGC 1 cut(s) 209
SspMI CTAG 2 cut(s) 333, 345
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 332
TaaI ACNGT 2 cut(s) 204, 520
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 240, 374
TasI AATT 1 cut(s) 443
Tru1I TTAA 3 cut(s) 99, 144, 522
Tru9I TTAA 3 cut(s) 99, 144, 522
TscAI CASTG 2 cut(s) 54, 218
TseFI GTSAC 1 cut(s) 483
TseI GCWGC 1 cut(s) 267
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 4 cut(s) 128, 194, 438, 456
TspRI CASTG 2 cut(s) 54, 218
Tth111I GACNNNGTC 1 cut(s) 202
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 239
XmaJI CCTAGG 1 cut(s) 332
XspI CTAG 2 cut(s) 333, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.