RchiOBHm_Chr2g0135201

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000353
Physical Location & Seq
Forward (+)
52246926 .. 52248992
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50615

Sequence Viewer

Length: 1311 bp
ATGGAAGCTGAGCTGAGGAGGCTTCAGTTAGAGCTCAAGCAAACAATGGACATGTATAATATAGCCTGCAAAGAGGCAATCACAGAAAAAAATAAGGCAAAGGAACTTAACCTATGGAAATTGGAAAAAGAATATAAATTAGAAGAGGCACGATTAGCTGAGGAAATGGCATTGAAACTAGTGGAGAAAGAGAAAGCAAAATGTAAGGCAGCTACCAAGGAAGCAGAAGCAGCTCAAAGGGTTGCCAAATTGGAAGCACAAAGAGCAAGGATTGCAGAAATGGAAGCTCTGAAACAATCAGAGGCGAAGAAGGTATCAGAAGCAATGACATATGATCTAAGGTACAGGAAATACACAATTGAGGAGCTTGAAGCAGCAACAAATGGTTTTTCACAAGCTTGCAAAATTGGTGAAGGAGGCTATGGGCCAGTATACCTGGGTGAAATAGACCATACACGAGTGGCAATAAAAGTTCTGCGTCCTGATGCAGCTCAAGGACGGTTGCAGTTCCAGAAAGAGGTTGAAATATTGAGCTGCATACGGCATCCAAACATGGTTCTCCTTCTTGGAGCCTGCCCTGAGTATGGTTGCCTGGTCTACGAGTACATGGCTAATGGTAGCTTAGAGGATTGTCTCTTCCGGCGATGTAACAAACAAGTTATTCCTTGGCAGCTAAGGTTCAGAATTGCTGCTGATATCTGCACTGGGCTTTTGTTTCTTCATCAAACTAAGCCAGAACCCCTAGTACACCGTGATCTAAAACCCGGAAACATTTTGCTAGACCATAACTATGTTGGCAAGATAAGTGATGTAGGTTTGGCCAGGCTTGTCCCTCAATCAGGATCTGATTCGGTCACTCAGTATTGCATGACTGCAACAGCAGGAACATTCTGTTATATCGATCCTGAGTATCAGCAAACAGGCATGCTTGTAACAAAGTCTGATGTCTACTCACTTGGTGTCATTCTTTTACAACTAATAACAGCCAACCCACCAATGGGTCTGACTCTTCAAGTTGAGAAAGCTATTGAGATGGGGAACTTTGCTCAAATTCTTGACCCTGCTGTTCCTGACTGGCCTGTTGAAGAAGCCTTAAAGTTTGCCAAACTAGGTTTACTGTGTGCAGAAATGAGGCGGAAAGACAGACCGGATCTTGGGAAGGTTGTGCTACCGGAGCTCAATAGATTGAGTGAACTTGCCCAAGATAGCATGAATGGTATCATGCGTAGTAGTAGAGGTGCTGGTGGAGTAATCTTACCAAGACAAGGACAACACCCCAGTTTGAAACTACAAGTGAGTATCGCAACCTAA

Protein Analysis

436

Amino Acids

48.85

Weight (kDa)

7.47

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 132 - 351 1e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 133 - 386 1.1e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 432, 597, 948
AciI CCGC 1 cut(s) 1135
AclWI GGATC 3 cut(s) 850, 896, 1158
AcoI YGGCCR 1 cut(s) 819
AcsI RAATTY 1 cut(s) 1050
AcuI CTGAAG 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 959
AfaI GTAC 3 cut(s) 344, 605, 747
AfiI CCNNNNNNNGG 7 cut(s) 517, 584, 839, 997, 998, 1154, 1265
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 6 cut(s) 175, 371, 524, 1013, 1085, 1285
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 3 cut(s) 435, 591, 821
Alw21I GWGCWC 2 cut(s) 36, 1179
Alw26I GTCTC 1 cut(s) 638
AlwI GGATC 3 cut(s) 850, 896, 1158
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 3 cut(s) 425, 819, 1076
ApeKI GCWGC 7 cut(s) 209, 230, 374, 488, 534, 670, 689
ApoI RAATTY 1 cut(s) 1050
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 2 cut(s) 422, 452
BalI TGGCCA 1 cut(s) 821
BanII GRGCYC 2 cut(s) 36, 1179
BauI CACGAG 1 cut(s) 456
Bbv12I GWGCWC 2 cut(s) 36, 1179
BbvCI CCTCAGC 2 cut(s) 14, 159
BbvI GCAGC 7 cut(s) 221, 242, 386, 500, 521, 676, 682
BccI CCATC 1 cut(s) 1027
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 3 cut(s) 437, 593, 823
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 1 cut(s) 638
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 4 cut(s) 179, 743, 779, 1109
BisI GCNGC 7 cut(s) 210, 231, 375, 489, 535, 671, 690
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 7 cut(s) 211, 232, 376, 490, 536, 672, 691
Bme1390I CCNGG 4 cut(s) 437, 593, 765, 823
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 4 cut(s) 437, 593, 765, 823
BmrI ACTGGG 2 cut(s) 714, 1272
BmsI GCATC 2 cut(s) 475, 553
BmuI ACTGGG 2 cut(s) 714, 1272
Bpu10I CCTNAGC 3 cut(s) 14, 159, 674
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 2 cut(s) 20, 477
BpuMI CCSGG 1 cut(s) 765
Bsa29I ATCGAT 1 cut(s) 900
BsaJI CCNNGG 3 cut(s) 216, 436, 665
BsaWI WCCGGW 2 cut(s) 1147, 1171
Bsc4I CCNNNNNNNGG 7 cut(s) 517, 584, 839, 997, 998, 1154, 1265
Bse1I ACTGG 4 cut(s) 428, 709, 1079, 1278
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 3 cut(s) 437, 593, 823
BseCI ATCGAT 1 cut(s) 900
BseDI CCNNGG 3 cut(s) 216, 436, 665
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 7 cut(s) 517, 584, 839, 997, 998, 1154, 1265
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 5 cut(s) 5, 150, 570, 872, 897
BseNI ACTGG 4 cut(s) 428, 709, 1079, 1278
BseRI GAGGAG 2 cut(s) 31, 377
BseXI GCAGC 7 cut(s) 221, 242, 386, 500, 521, 676, 682
BsgI GTGCAG 2 cut(s) 685, 1143
BshFI GGCC 3 cut(s) 427, 821, 1078
BshVI ATCGAT 1 cut(s) 900
BsiHKAI GWGCWC 2 cut(s) 36, 1179
BsiSI CCGG 4 cut(s) 640, 765, 1148, 1172
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 7 cut(s) 517, 584, 839, 997, 998, 1154, 1265
BsmAI GTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 3 cut(s) 427, 821, 1078
Bsp1286I GDGCHC 2 cut(s) 36, 1179
Bsp143I GATC 5 cut(s) 334, 754, 842, 901, 1150
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 1 cut(s) 1135
BspANI GGCC 3 cut(s) 427, 821, 1078
BspCNI CTCAG 5 cut(s) 6, 151, 571, 871, 898
BspDI ATCGAT 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 571
BspPI GGATC 3 cut(s) 850, 896, 1158
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 4 cut(s) 428, 709, 1079, 1278
BssECI CCNNGG 3 cut(s) 216, 436, 665
BssMI GATC 5 cut(s) 334, 754, 842, 901, 1150
BssNAI GTATAC 1 cut(s) 433
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 2 cut(s) 216, 665
Bst1107I GTATAC 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 3 cut(s) 437, 593, 823
Bst4CI ACNGT 3 cut(s) 501, 752, 1119
Bst6I CTCTTC 3 cut(s) 138, 641, 1014
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 4 cut(s) 67, 400, 574, 926
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 5 cut(s) 337, 757, 845, 904, 1153
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 5 cut(s) 334, 754, 842, 901, 1150
BstMWI GCNNNNNNNGC 6 cut(s) 19, 155, 230, 263, 272, 1174
BstNI CCWGG 3 cut(s) 437, 593, 823
BstNSI RCATGY 2 cut(s) 55, 928
BstSCI CCNGG 4 cut(s) 435, 591, 763, 821
BstV1I GCAGC 7 cut(s) 221, 242, 386, 500, 521, 676, 682
BstX2I RGATCY 2 cut(s) 842, 1150
BstYI RGATCY 2 cut(s) 842, 1150
BstZ17I GTATAC 1 cut(s) 433
Bsu15I ATCGAT 1 cut(s) 900
BsuRI GGCC 3 cut(s) 427, 821, 1078
BsuTUI ATCGAT 1 cut(s) 900
BtgZI GCGATG 1 cut(s) 658
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 4 cut(s) 67, 400, 574, 926
CaiI CAGNNNCTG 1 cut(s) 845
Cfr13I GGNCC 1 cut(s) 425
ClaI ATCGAT 1 cut(s) 900
CseI GACGC 1 cut(s) 467
Csp6I GTAC 3 cut(s) 343, 604, 746
CviAII CATG 7 cut(s) 52, 553, 607, 868, 925, 1210, 1222
CviQI GTAC 3 cut(s) 343, 604, 746
DpnI GATC 5 cut(s) 336, 756, 844, 903, 1152
DpnII GATC 5 cut(s) 334, 754, 842, 901, 1150
DraIII CACNNNGTG 1 cut(s) 959
EaeI YGGCCR 1 cut(s) 819
Eam1104I CTCTTC 3 cut(s) 138, 641, 1014
EarI CTCTTC 3 cut(s) 138, 641, 1014
EciI GGCGGA 1 cut(s) 1150
Ecl136II GAGCTC 2 cut(s) 34, 1177
Eco130I CCWWGG 2 cut(s) 216, 665
Eco24I GRGCYC 2 cut(s) 36, 1179
Eco32I GATATC 1 cut(s) 697
Eco53kI GAGCTC 2 cut(s) 34, 1177
Eco57I CTGAAG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 34, 1177
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRII CCWGG 3 cut(s) 435, 591, 821
EcoRV GATATC 1 cut(s) 697
EcoT14I CCWWGG 2 cut(s) 216, 665
EcoT38I GRGCYC 2 cut(s) 36, 1179
ErhI CCWWGG 2 cut(s) 216, 665
FaeI CATG 7 cut(s) 55, 556, 610, 871, 928, 1213, 1225
FaqI GGGAC 1 cut(s) 815
FatI CATG 7 cut(s) 51, 552, 606, 867, 924, 1209, 1221
FauNDI CATATG 1 cut(s) 331
FblI GTMKAC 3 cut(s) 432, 597, 948
Fnu4HI GCNGC 7 cut(s) 210, 231, 375, 489, 535, 671, 690
FokI GGATG 1 cut(s) 531
FriOI GRGCYC 2 cut(s) 36, 1179
Fsp4HI GCNGC 7 cut(s) 210, 231, 375, 489, 535, 671, 690
FspBI CTAG 4 cut(s) 179, 743, 779, 1109
GluI GCNGC 7 cut(s) 210, 231, 375, 489, 535, 671, 690
HaeIII GGCC 3 cut(s) 427, 821, 1078
HapII CCGG 4 cut(s) 640, 765, 1148, 1172
HgaI GACGC 1 cut(s) 467
Hin1II CATG 7 cut(s) 55, 556, 610, 871, 928, 1213, 1225
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 2 cut(s) 848, 1006
HpaII CCGG 4 cut(s) 640, 765, 1148, 1172
HphI GGTGA 2 cut(s) 422, 452
Hpy166II GTNNAC 6 cut(s) 433, 598, 748, 949, 1115, 1193
Hpy188I TCNGA 7 cut(s) 291, 301, 319, 683, 847, 943, 1005
Hpy188III TCNNGA 6 cut(s) 482, 511, 840, 905, 1055, 1070
Hpy8I GTNNAC 6 cut(s) 433, 598, 748, 949, 1115, 1193
HpyAV CCTTC 4 cut(s) 304, 407, 572, 1153
HpyCH4III ACNGT 3 cut(s) 501, 752, 1119
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 155, 230, 263, 272, 1174
Hsp92II CATG 7 cut(s) 55, 556, 610, 871, 928, 1213, 1225
Kzo9I GATC 5 cut(s) 334, 754, 842, 901, 1150
LmnI GCTCC 3 cut(s) 364, 569, 1174
Lsp1109I GCAGC 7 cut(s) 221, 242, 386, 500, 521, 676, 682
LweI GCATC 2 cut(s) 475, 553
MaeI CTAG 4 cut(s) 179, 743, 779, 1109
MaeIII GTNAC 3 cut(s) 647, 853, 931
MalI GATC 5 cut(s) 336, 756, 844, 903, 1152
MboI GATC 5 cut(s) 334, 754, 842, 901, 1150
MboII GAAGA 6 cut(s) 155, 319, 628, 710, 1001, 1097
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 2 cut(s) 842, 1150
MhlI GDGCHC 2 cut(s) 36, 1179
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 7 cut(s) 119, 137, 248, 357, 405, 684, 1050
MluNI TGGCCA 1 cut(s) 821
MlyI GAGTC 1 cut(s) 1000
Mox20I TGGCCA 1 cut(s) 821
MscI TGGCCA 1 cut(s) 821
MseI TTAA 2 cut(s) 108, 1094
MslI CAYNNNNRTG 1 cut(s) 789
Msp20I TGGCCA 1 cut(s) 821
MspI CCGG 4 cut(s) 640, 765, 1148, 1172
MspR9I CCNGG 4 cut(s) 437, 593, 765, 823
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 3 cut(s) 437, 593, 823
MwoI GCNNNNNNNGC 6 cut(s) 19, 155, 230, 263, 272, 1174
NciI CCSGG 1 cut(s) 765
NdeI CATATG 1 cut(s) 331
NdeII GATC 5 cut(s) 334, 754, 842, 901, 1150
NlaIII CATG 7 cut(s) 55, 556, 610, 871, 928, 1213, 1225
NlaIV GGNNCC 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 2 cut(s) 55, 928
PaeI GCATGC 1 cut(s) 928
PciI ACATGT 1 cut(s) 51
PfeI GAWTC 1 cut(s) 848
PkrI GCNGC 7 cut(s) 211, 232, 376, 490, 536, 672, 691
PleI GAGTC 1 cut(s) 1000
PpsI GAGTC 1 cut(s) 1000
PscI ACATGT 1 cut(s) 51
Psp124BI GAGCTC 2 cut(s) 36, 1179
Psp6I CCWGG 3 cut(s) 435, 591, 821
PspGI CCWGG 3 cut(s) 435, 591, 821
PspN4I GGNNCC 1 cut(s) 571
PspPI GGNCC 1 cut(s) 425
PsrI GAACNNNNNNTAC 2 cut(s) 729, 761
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 2 cut(s) 842, 1150
RsaI GTAC 3 cut(s) 344, 605, 747
RsaNI GTAC 3 cut(s) 343, 604, 746
RseI CAYNNNNRTG 1 cut(s) 789
SacI GAGCTC 2 cut(s) 36, 1179
SaqAI TTAA 2 cut(s) 108, 1094
SatI GCNGC 7 cut(s) 210, 231, 375, 489, 535, 671, 690
Sau3AI GATC 5 cut(s) 334, 754, 842, 901, 1150
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 1000
ScrFI CCNGG 4 cut(s) 437, 593, 765, 823
SduI GDGCHC 2 cut(s) 36, 1179
SfaNI GCATC 2 cut(s) 475, 553
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 2 cut(s) 35, 492
SmoI CTYRAG 2 cut(s) 35, 492
SpeI ACTAGT 1 cut(s) 178
SphI GCATGC 1 cut(s) 928
Sse9I AATT 7 cut(s) 119, 137, 248, 357, 405, 684, 1050
SsiI CCGC 1 cut(s) 1135
SspI AATATT 1 cut(s) 528
SspMI CTAG 4 cut(s) 179, 743, 779, 1109
SstI GAGCTC 2 cut(s) 36, 1179
StyD4I CCNGG 4 cut(s) 435, 591, 763, 821
StyI CCWWGG 2 cut(s) 216, 665
TaaI ACNGT 3 cut(s) 501, 752, 1119
TaqI TCGA 1 cut(s) 900
TaqII GACCGA 1 cut(s) 841
TasI AATT 7 cut(s) 119, 137, 248, 357, 405, 684, 1050
TatI WGTACW 2 cut(s) 603, 745
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 2 cut(s) 108, 1094
Tru9I TTAA 2 cut(s) 108, 1094
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 7 cut(s) 209, 230, 374, 488, 534, 670, 689
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 2 cut(s) 710, 1226
TspRI CASTG 1 cut(s) 709
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 1 cut(s) 1050
XceI RCATGY 2 cut(s) 55, 928
XcmI CCANNNNNNNNNTGG 2 cut(s) 791, 994
XmiI GTMKAC 3 cut(s) 432, 597, 948
XspI CTAG 4 cut(s) 179, 743, 779, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.