Rh4BG077700

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000353
Physical Location & Seq
Forward (+)
13720323 .. 13725116
4794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG077700.1

Sequence Viewer

Length: 2268 bp
ATGGTAAGAGAAATGAGAAGAGAGAAAGCAGAAAACAGTACCATAGAGGCGGTGGCAGTCGCAATCGACAAGGACAGAGGAAGTCACCATGCTTTCAAATGGACAGTTGAAAATCTCCTCACCAAAGGCCAATCTATTACACTTGTTCATGTTAAACATATAACATCTGCAAATTCCACACAATCCAGGGGCATTCCCACGTCTGGAGAGAAGGAGGAGTTCAAGATAGTCAACAAAAACCAATTTGATGCTAAGATCAACGAGTTGTTTCTTCCATTCCGCTGGTTTTGCGTAAAAAGAGCTATAAAATGCAATGAAGTTGTAATTGAGAACCCTGATGTACCAAAAGCACTGATTAACTATGTTTCCGCTAATTTAGTTGACATTTTGGCACTTGGGGCACCATCGACAAGATACGGCCTTCTGCGGTTCAAAAGTACAAATGTTTCAACCACAGTGTCAAAGGCAGCACCAGAGTTCTGCACTGTGTATGTCGTCGGAAAAGGAAAGTTATCATATGTGCGATCTGCTAACTGTTGTCCACACCCAAAAGCTCCTTCATGTAAACAAATACAGCACCAATACAGCAATGTTTCTGAAGCAAATAAAAGCAACCAGCACCCCAGAGAATCACAGAGGACACAGTATCTAGCCAATAGCCATCTAGATAACATTGAAATCAAGTCACCATTCACAAAAGGAAACGCTTCAATGAACAAATCATATGAACTCTCGACAGAAACTGATATATCGTTTGTCAGTAGTGGAAGGCCAAGCACAGACTGCATGTTTCCTGCTTTCTCTGAGAGTCCAGAATCAGGACCTCGACATTCAGTCAGTTCATATATGGACTACAGAAGACCATCTTATTCAGGAGGCAAGTTTATTGACATGAGCTCTTCACAATGTGAAGTCTCATCATCCTCAATTAAAAGTGGAAACTCATGGTTATCACAGAATATGGATGAAATGGAAGCTGAGATGAGGAGGCTCAGGCTAGAGCTTAAGCGAACAATGGACATGTACAGTACGGCCTGCAAGGAAGCACTCACTGAAAAACAAAAGGCAATGCAACTTCACCGATTGAAAATGGAAGAAAAGCAGAGATTAGAAGAGGCACGAGAAGCTGAGGAAGCTGCATTGAAACTTGCAGGGAAGGAGAAAGCAAAATGTCAGGCAGCAATGGATGCTGCAGAAGCAGCTAAAAGGATTGCAGAAGCAGAAGCACAACAGAGGAGGAATGCTGAAATGAAAGCCCTTAAAGAAGCTGAAGAGAGGATGAAGGCACTAGGAACAACGACGTACGATCCCAGATACAGGAGATACACAATTGAGGAGATTGAAGTAGCAACAAATAATTTTTCACACACTCTAGAGGTTGGGGAAGGAGGCTATGGGCCAGTATACAAAGGTGAACTAGACCACACACCAGTGGCAATAAAAGTTCTACGTTCAGATGCAGCTGAAGGATGGTCCCAATTCCAGCAAGAGGTTCATATATTGAGTTGCATACGCCATCCCAACATAATTCTTCTTCTTGGAGCCTGCCCAGAGAATGGATGCCTGGTTTATGAGTACATGGATAATGGGAGCTTAGAAGACCGTCTCTTTCGGCGAGGTAACACCCCAGTTATTCCTTGGCAGCTAAGGTTCCAAATCGCTGCAGATATTGGCACAGGACTGTTGTTCCTTCATCAGACCAAGCCAGAACCACTTGTACACCGTGACCTAAAACCCAGCAACATTTTACTAGACTATAACTATGTCAGCAAGATTTGTGATGTAGGTTTGGCCAGGCTTGTCCCTCCATCAGTAGCTGATTCTATCACTCAATATCATATGACATCTGCCGCCGGAACCTTCTGTTATATTGATCCAGAGTATCAGCAAACTGGCATGCTTGGGATAAAATCTGATATATACTCACTTGGGGTCATGTTCCTACAACTAATAACAGCCAAACCACCTATGGGTTTGACTCATCACGTTGAGCGGGCTATTGAGAAAGGGACTTTCTCTGAGATGCTTGACCCTGCCGTTCCTGACTGGCCAGTTGAGGAAGCATTGTGTTTTGCAAAGCTAGCTCTACAGTGTGCTGAAATGAGGCAAAAAGATCGACCGGATCTTGGGAAGATTGTTCTTCTTGAGCTTAACAGATTGAGAAAATTAGGCGAGGATAGCATGAATGGCGTTACGGTTGGTAATGATGAAGTATCTACAGCTAGACAAGTGAGTAGCATAGCAGACCTAGCATTGCACTTGAAGTAA

Protein Analysis

755

Amino Acids

84.57

Weight (kDa)

7.27

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 17 - 165 5.6e-06 Universal stress protein family
Pkinase PF00069 459 - 708 2.1e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 459 - 713 1.1e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 1556
AccBSI CCGCTC 1 cut(s) 1993
AccI GTMKAC 1 cut(s) 1404
AciI CCGC 6 cut(s) 50, 280, 369, 427, 1851, 1993
AclWI GGATC 3 cut(s) 1301, 1868, 2130
AcoI YGGCCR 2 cut(s) 1791, 2048
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 3 cut(s) 618, 1290, 1485
AdeI CACNNNGTG 1 cut(s) 908
AfaI GTAC 8 cut(s) 40, 342, 439, 1025, 1030, 1304, 1577, 1719
AfiI CCNNNNNNNGG 7 cut(s) 203, 818, 1317, 1489, 1556, 1970, 2126
AflII CTTAAG 1 cut(s) 1004
AflIII ACRYGT 1 cut(s) 1020
AhdI GACNNNNNGTC 1 cut(s) 833
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 3 cut(s) 185, 1563, 1793
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
AleI CACNNNNGTG 1 cut(s) 1430
Alw21I GWGCWC 1 cut(s) 899
Alw26I GTCTC 2 cut(s) 919, 1610
AlwI GGATC 3 cut(s) 1301, 1868, 2130
AlwNI CAGNNNCTG 2 cut(s) 743, 1817
AoxI GGCC 7 cut(s) 127, 418, 770, 1032, 1397, 1791, 2048
ApeKI GCWGC 8 cut(s) 467, 1136, 1178, 1190, 1199, 1460, 1642, 1661
ApoI RAATTY 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 706
AspS9I GGNCC 3 cut(s) 821, 1397, 1473
AsuHPI GGTGA 5 cut(s) 77, 112, 678, 1070, 1424
AsuNHI GCTAGC 1 cut(s) 2080
AvaII GGWCC 2 cut(s) 821, 1473
BaeGI GKGCMC 1 cut(s) 403
BalI TGGCCA 2 cut(s) 1793, 2050
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 899
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 2 cut(s) 865, 1605
Bbv12I GWGCWC 1 cut(s) 899
BbvCI CCTCAGC 1 cut(s) 1128
BbvI GCAGC 8 cut(s) 479, 1123, 1177, 1190, 1211, 1472, 1648, 1654
BccI CCATC 6 cut(s) 412, 669, 871, 1464, 1524, 1816
BceAI ACGGC 3 cut(s) 433, 1047, 2021
BcgI CGANNNNNNTGC 2 cut(s) 2096, 2130
BciT130I CCWGG 3 cut(s) 187, 1565, 1795
BcoDI GTCTC 2 cut(s) 919, 1610
BfmI CTRYAG 5 cut(s) 853, 1191, 1662, 2087, 2217
BfrI CTTAAG 1 cut(s) 1004
BisI GCNGC 9 cut(s) 468, 1137, 1179, 1191, 1200, 1461, 1643, 1662, 1851
BlsI GCNGC 9 cut(s) 469, 1138, 1180, 1192, 1201, 1462, 1644, 1663, 1852
Bme1390I CCNGG 3 cut(s) 187, 1565, 1795
Bme18I GGWCC 2 cut(s) 821, 1473
BmeRI GACNNNNNGTC 1 cut(s) 833
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 3 cut(s) 821, 1397, 1473
BmiI GGNNCC 5 cut(s) 402, 1475, 1543, 1652, 1858
BmrFI CCNGG 3 cut(s) 187, 1565, 1795
BmrI ACTGGG 1 cut(s) 1622
BmsI GCATC 5 cut(s) 238, 1177, 1447, 1550, 2013
BmtI GCTAGC 1 cut(s) 2084
BmuI ACTGGG 1 cut(s) 1622
BpiI GAAGAC 2 cut(s) 865, 1605
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 3 cut(s) 992, 1128, 1646
BpuEI CTTGAG 1 cut(s) 2165
BsaJI CCNNGG 2 cut(s) 186, 1637
BsaWI WCCGGW 1 cut(s) 2119
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 818, 1317, 1489, 1556, 1970, 2126
Bse1I ACTGG 6 cut(s) 1400, 1430, 1628, 1897, 2051, 2051
Bse3DI GCAATG 5 cut(s) 319, 595, 1074, 1188, 2252
BseBI CCWGG 3 cut(s) 187, 1565, 1795
BseDI CCNNGG 2 cut(s) 186, 1637
BseGI GGATG 7 cut(s) 920, 970, 1192, 1284, 1475, 1516, 1565
BseLI CCNNNNNNNGG 7 cut(s) 203, 818, 1317, 1489, 1556, 1970, 2126
BseMI GCAATG 5 cut(s) 319, 595, 1074, 1188, 2252
BseMII CTCAG 5 cut(s) 795, 969, 1006, 1119, 2010
BseNI ACTGG 6 cut(s) 1400, 1430, 1628, 1897, 2051, 2051
BseRI GAGGAG 5 cut(s) 107, 230, 1000, 1249, 1349
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 8 cut(s) 479, 1123, 1177, 1190, 1211, 1472, 1648, 1654
BseYI CCCAGC 1 cut(s) 1736
BsgI GTGCAG 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 2120
BshFI GGCC 7 cut(s) 129, 420, 772, 1034, 1399, 1793, 2050
BshNI GGYRCC 1 cut(s) 400
BsiEI CGRYCG 1 cut(s) 2120
BsiHKAI GWGCWC 1 cut(s) 899
BsiSI CCGG 2 cut(s) 1854, 2120
BsiWI CGTACG 1 cut(s) 1302
BslFI GGGAC 3 cut(s) 1459, 1787, 2023
BslI CCNNNNNNNGG 7 cut(s) 203, 818, 1317, 1489, 1556, 1970, 2126
BsmAI GTCTC 2 cut(s) 919, 1610
BsmBI CGTCTC 1 cut(s) 1610
BsmFI GGGAC 3 cut(s) 1459, 1787, 2023
BsmI GAATGC 2 cut(s) 192, 1246
BsnI GGCC 7 cut(s) 129, 420, 772, 1034, 1399, 1793, 2050
Bsp1286I GDGCHC 2 cut(s) 403, 899
Bsp1407I TGTACA 2 cut(s) 1023, 1717
Bsp143I GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
BspACI CCGC 6 cut(s) 50, 280, 369, 427, 1851, 1993
BspANI GGCC 7 cut(s) 129, 420, 772, 1034, 1399, 1793, 2050
BspCNI CTCAG 5 cut(s) 796, 970, 1005, 1120, 2011
BspLI GGNNCC 5 cut(s) 402, 1475, 1543, 1652, 1858
BspMAI CTGCAG 2 cut(s) 1195, 1666
BspOI GCTAGC 1 cut(s) 2084
BspPI GGATC 3 cut(s) 1301, 1868, 2130
BspQI GCTCTTC 1 cut(s) 904
BspT107I GGYRCC 1 cut(s) 400
BspTI CTTAAG 1 cut(s) 1004
BsrBI CCGCTC 1 cut(s) 1993
BsrDI GCAATG 5 cut(s) 319, 595, 1074, 1188, 2252
BsrGI TGTACA 2 cut(s) 1023, 1717
BsrI ACTGG 6 cut(s) 1400, 1430, 1628, 1897, 2051, 2051
BssECI CCNNGG 2 cut(s) 186, 1637
BssMI GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
BssNAI GTATAC 1 cut(s) 1405
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 1637
Bst1107I GTATAC 1 cut(s) 1405
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 3 cut(s) 187, 1565, 1795
Bst6I CTCTTC 4 cut(s) 13, 904, 1107, 1266
BstAFI CTTAAG 1 cut(s) 1004
BstAPI GCANNNNNTGC 2 cut(s) 783, 1187
BstAUI TGTACA 2 cut(s) 1023, 1717
BstC8I GCNNGC 5 cut(s) 1036, 1546, 1898, 1995, 2082
BstDEI CTNAG 8 cut(s) 252, 804, 978, 992, 1128, 1594, 1646, 2019
BstF5I GGATG 7 cut(s) 920, 970, 1192, 1284, 1475, 1516, 1565
BstKTI GATC 6 cut(s) 258, 527, 1309, 1876, 2116, 2125
BstMAI GTCTC 2 cut(s) 919, 1610
BstMBI GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
BstMCI CGRYCG 1 cut(s) 2120
BstNI CCWGG 3 cut(s) 187, 1565, 1795
BstNSI RCATGY 3 cut(s) 790, 1024, 1900
BstSCI CCNGG 3 cut(s) 185, 1563, 1793
BstSFI CTRYAG 5 cut(s) 853, 1191, 1662, 2087, 2217
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 8 cut(s) 479, 1123, 1177, 1190, 1211, 1472, 1648, 1654
BstV2I GAAGAC 2 cut(s) 865, 1605
BstX2I RGATCY 1 cut(s) 2122
BstXI CCANNNNNNTGG 1 cut(s) 282
BstYI RGATCY 1 cut(s) 2122
BstZ17I GTATAC 1 cut(s) 1405
BsuRI GGCC 7 cut(s) 129, 420, 772, 1034, 1399, 1793, 2050
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 7 cut(s) 920, 970, 1192, 1284, 1475, 1516, 1565
BtsIMutI CAGTG 6 cut(s) 350, 462, 483, 1050, 1437, 2096
Cac8I GCNNGC 5 cut(s) 1036, 1546, 1898, 1995, 2082
CaiI CAGNNNCTG 2 cut(s) 743, 1817
Cfr13I GGNCC 3 cut(s) 821, 1397, 1473
Csp6I GTAC 8 cut(s) 39, 341, 438, 1024, 1029, 1303, 1576, 1718
CviQI GTAC 8 cut(s) 39, 341, 438, 1024, 1029, 1303, 1576, 1718
DdeI CTNAG 8 cut(s) 252, 804, 978, 992, 1128, 1594, 1646, 2019
DpnI GATC 6 cut(s) 257, 526, 1308, 1875, 2115, 2124
DpnII GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
DraIII CACNNNGTG 1 cut(s) 908
DriI GACNNNNNGTC 1 cut(s) 833
EaeI YGGCCR 2 cut(s) 1791, 2048
Eam1104I CTCTTC 4 cut(s) 13, 904, 1107, 1266
Eam1105I GACNNNNNGTC 1 cut(s) 833
EarI CTCTTC 4 cut(s) 13, 904, 1107, 1266
Ecl136II GAGCTC 1 cut(s) 897
Eco130I CCWWGG 1 cut(s) 1637
Eco24I GRGCYC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 821, 1473
Eco53kI GAGCTC 1 cut(s) 897
Eco57I CTGAAG 3 cut(s) 618, 1290, 1485
EcoICRI GAGCTC 1 cut(s) 897
EcoO109I RGGNCCY 1 cut(s) 821
EcoRII CCWGG 3 cut(s) 185, 1563, 1793
EcoT14I CCWWGG 1 cut(s) 1637
EcoT38I GRGCYC 1 cut(s) 899
ErhI CCWWGG 1 cut(s) 1637
Esp3I CGTCTC 1 cut(s) 1610
FalI AAGNNNNNCTT 2 cut(s) 850, 882
FaqI GGGAC 3 cut(s) 1459, 1787, 2023
FauI CCCGC 1 cut(s) 1986
FauNDI CATATG 3 cut(s) 517, 724, 1839
FblI GTMKAC 1 cut(s) 1404
Fnu4HI GCNGC 9 cut(s) 468, 1137, 1179, 1191, 1200, 1461, 1643, 1662, 1851
FokI GGATG 7 cut(s) 907, 977, 1199, 1291, 1482, 1503, 1572
FriOI GRGCYC 1 cut(s) 899
Fsp4HI GCNGC 9 cut(s) 468, 1137, 1179, 1191, 1200, 1461, 1643, 1662, 1851
GluI GCNGC 9 cut(s) 468, 1137, 1179, 1191, 1200, 1461, 1643, 1662, 1851
GsaI CCCAGC 1 cut(s) 1740
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 7 cut(s) 129, 420, 772, 1034, 1399, 1793, 2050
HapII CCGG 2 cut(s) 1854, 2120
HincII GTYRAC 2 cut(s) 232, 382
HindII GTYRAC 2 cut(s) 232, 382
HinfI GANTC 5 cut(s) 629, 808, 815, 1820, 1978
HpaII CCGG 2 cut(s) 1854, 2120
HphI GGTGA 5 cut(s) 77, 112, 678, 1070, 1424
Hpy166II GTNNAC 7 cut(s) 232, 382, 542, 566, 1405, 1415, 1720
Hpy188I TCNGA 7 cut(s) 500, 598, 805, 1456, 1698, 1915, 2020
Hpy8I GTNNAC 7 cut(s) 232, 382, 542, 566, 1405, 1415, 1720
Hpy99I CGWCG 2 cut(s) 500, 1303
HpyCH4IV ACGT 4 cut(s) 200, 1301, 1450, 1986
HpyF3I CTNAG 8 cut(s) 252, 804, 978, 992, 1128, 1594, 1646, 2019
HpySE526I ACGT 4 cut(s) 200, 1301, 1450, 1986
Kzo9I GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
LguI GCTCTTC 1 cut(s) 904
LmnI GCTCC 3 cut(s) 559, 1541, 1590
Lsp1109I GCAGC 8 cut(s) 479, 1123, 1177, 1190, 1211, 1472, 1648, 1654
LweI GCATC 5 cut(s) 238, 1177, 1447, 1550, 2013
MaeII ACGT 4 cut(s) 200, 1301, 1450, 1986
MaeIII GTNAC 5 cut(s) 83, 684, 1619, 1724, 2191
MalI GATC 6 cut(s) 257, 526, 1308, 1875, 2115, 2124
MbiI CCGCTC 1 cut(s) 1993
MboI GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 1 cut(s) 2122
MhlI GDGCHC 2 cut(s) 403, 899
MlsI TGGCCA 2 cut(s) 1793, 2050
MluNI TGGCCA 2 cut(s) 1793, 2050
MlyI GAGTC 2 cut(s) 817, 1972
MmeI TCCRAC 1 cut(s) 478
Mox20I TGGCCA 2 cut(s) 1793, 2050
MroXI GAANNNNTTC 1 cut(s) 706
MscI TGGCCA 2 cut(s) 1793, 2050
MseI TTAA 6 cut(s) 153, 357, 930, 1005, 1260, 2151
MslI CAYNNNNRTG 1 cut(s) 1430
Msp20I TGGCCA 2 cut(s) 1793, 2050
MspA1I CMGCKG 2 cut(s) 282, 1463
MspCI CTTAAG 1 cut(s) 1004
MspI CCGG 2 cut(s) 1854, 2120
MspR9I CCNGG 3 cut(s) 187, 1565, 1795
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 2 cut(s) 192, 1246
MvaI CCWGG 3 cut(s) 187, 1565, 1795
NdeI CATATG 3 cut(s) 517, 724, 1839
NdeII GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
NheI GCTAGC 1 cut(s) 2080
NlaIV GGNNCC 5 cut(s) 402, 1475, 1543, 1652, 1858
NmuCI GTSAC 3 cut(s) 83, 684, 1724
NspI RCATGY 3 cut(s) 790, 1024, 1900
OliI CACNNNNGTG 1 cut(s) 1430
PaeI GCATGC 1 cut(s) 1900
PciI ACATGT 1 cut(s) 1020
PciSI GCTCTTC 1 cut(s) 904
PctI GAATGC 2 cut(s) 192, 1246
PdmI GAANNNNTTC 1 cut(s) 706
PfeI GAWTC 3 cut(s) 629, 815, 1820
Pfl23II CGTACG 1 cut(s) 1302
PflMI CCANNNNNTGG 1 cut(s) 1556
PkrI GCNGC 9 cut(s) 469, 1138, 1180, 1192, 1201, 1462, 1644, 1663, 1852
PleI GAGTC 2 cut(s) 816, 1972
PpsI GAGTC 2 cut(s) 816, 1972
PpuMI RGGWCCY 1 cut(s) 821
PscI ACATGT 1 cut(s) 1020
Psp124BI GAGCTC 1 cut(s) 899
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 3 cut(s) 185, 1563, 1793
PspFI CCCAGC 1 cut(s) 1736
PspGI CCWGG 3 cut(s) 185, 1563, 1793
PspLI CGTACG 1 cut(s) 1302
PspN4I GGNNCC 5 cut(s) 402, 1475, 1543, 1652, 1858
PspPI GGNCC 3 cut(s) 821, 1397, 1473
PspPPI RGGWCCY 1 cut(s) 821
PsrI GAACNNNNNNTAC 2 cut(s) 1701, 1733
PstI CTGCAG 2 cut(s) 1195, 1666
PstNI CAGNNNCTG 2 cut(s) 743, 1817
PsuI RGATCY 1 cut(s) 2122
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 8 cut(s) 40, 342, 439, 1025, 1030, 1304, 1577, 1719
RsaNI GTAC 8 cut(s) 39, 341, 438, 1024, 1029, 1303, 1576, 1718
RseI CAYNNNNRTG 1 cut(s) 1430
SacI GAGCTC 1 cut(s) 899
SapI GCTCTTC 1 cut(s) 904
SaqAI TTAA 6 cut(s) 153, 357, 930, 1005, 1260, 2151
SatI GCNGC 9 cut(s) 468, 1137, 1179, 1191, 1200, 1461, 1643, 1662, 1851
Sau3AI GATC 6 cut(s) 255, 524, 1306, 1873, 2113, 2122
Sau96I GGNCC 3 cut(s) 821, 1397, 1473
SchI GAGTC 2 cut(s) 817, 1972
ScrFI CCNGG 3 cut(s) 187, 1565, 1795
SduI GDGCHC 2 cut(s) 403, 899
SfaNI GCATC 5 cut(s) 238, 1177, 1447, 1550, 2013
SfcI CTRYAG 5 cut(s) 853, 1191, 1662, 2087, 2217
SinI GGWCC 2 cut(s) 821, 1473
SmiMI CAYNNNNRTG 1 cut(s) 1430
SmlI CTYRAG 2 cut(s) 1004, 2144
SmoI CTYRAG 2 cut(s) 1004, 2144
SphI GCATGC 1 cut(s) 1900
SsiI CCGC 6 cut(s) 50, 280, 369, 427, 1851, 1993
SstI GAGCTC 1 cut(s) 899
StyD4I CCNGG 3 cut(s) 185, 1563, 1793
StyI CCWWGG 1 cut(s) 1637
TaiI ACGT 4 cut(s) 203, 1304, 1453, 1989
TaqI TCGA 5 cut(s) 66, 407, 734, 826, 2116
TatI WGTACW 4 cut(s) 437, 1023, 1575, 1717
TauI GCSGC 1 cut(s) 1853
TfiI GAWTC 3 cut(s) 629, 815, 1820
Tru1I TTAA 6 cut(s) 153, 357, 930, 1005, 1260, 2151
Tru9I TTAA 6 cut(s) 153, 357, 930, 1005, 1260, 2151
TscAI CASTG 6 cut(s) 357, 462, 490, 1057, 1437, 2096
TseFI GTSAC 3 cut(s) 83, 684, 1724
TseI GCWGC 8 cut(s) 467, 1136, 1178, 1190, 1199, 1460, 1642, 1661
Tsp45I GTSAC 3 cut(s) 83, 684, 1724
TspRI CASTG 6 cut(s) 357, 462, 490, 1057, 1437, 2096
Van91I CCANNNNNTGG 1 cut(s) 1556
Vha464I CTTAAG 1 cut(s) 1004
VpaK11BI GGWCC 2 cut(s) 821, 1473
XapI RAATTY 1 cut(s) 172
XbaI TCTAGA 2 cut(s) 664, 1372
XceI RCATGY 3 cut(s) 790, 1024, 1900
XcmI CCANNNNNNNNNTGG 3 cut(s) 49, 1635, 1966
XmiI GTMKAC 1 cut(s) 1404
XmnI GAANNNNTTC 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.