Prupe.3G091100_v2.0.a1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000353
Physical Location & Seq
Forward (+)
6785974 .. 6789801
3828 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G091100.1

Sequence Viewer

Length: 2373 bp
ATGGGACTTAGAGGGAAACCTGGAAAGAAGGAAGAGTCTGTGGCTGTGGCAGTAGACAAGGACAAAGGAAGTCAGCATGCTATCAAATGGACTGTTGATAATCTTCTCAGCAGGGGCCAAAATGTCACATTGCTCCATGTCAAAGAAACTGCATCTCACCACCATAACCACCATCACCATACCGGGCATGGGACAGAGGAGGCCGGAAAGGTGCACCAAAGCCAAGCTGATGCTCAAGCCAAAGAATTGTTTCTTCCATTCCGCGGCTATTGCACCAAAAAGGCTATAAAATGCCATGAGATCATAATTGAGGACAATGATATATCTAAAGCCCTCGTCAACTATGTTACCACTAATTTAATTGAGATTTTGGTGCTTGGCACACCATCAAAGAATGGTCTTTTCAGGTTCATGACCACGGATGTTCCAACCATTGTGTCGAAAAAGGCACCAGACTTTTGCACGGTGTATGTCATTGGCCGGGGAAAAATCTCAATTGTGCGATCCGCTACCGGTTCAGTACCTGAAAGAGTCTCACCGCCACCTCAAGAAATAGAGCATCACTCCAGCAAAGCTTCTGAATCTTATCCACAAATGAGGATGAGCAATCATAAACCCAGAGCACCAGAAAGGACACAGTTTTCAGCCCGTTGGCATCCAAACGAAAACGAAATCATCAGAGGAAACTCTGCACTGAACAAATCATATGAGCTTCCACCGGACAGTGATATATCTTATGTAAGCAGCGGAACGCCAGGCAGGGATCACATGTTTCCTTCATTCTATGACAGCATGTCATCGGGAATGACCTCGCGTTTTTCAATCAACTCCGATTTGGACAGCAGAAGCTCCACCTCTACGGCATCCTACTCTGGAACCAAGTTTATCGACATGAGCTCTCTGCAACATGATTTCTCATCACCACTTCGTAATTTATCATCACCGCATCATGATTTCTCATCACCGCAGCATGATTTCTCATCAAGCTCATTTGAAAGTGGAAACTCGTGGTCATCGTCACAGAACGCGGATGATGTGGACGCCGAGATGAGGAGGCTCAGGCTAGAGCTCAAACACACAAGGGATATGTACAATACAGCCTGCAAGGAGGGAGTCACAGAAAAACATAAGGGAAAGGAAGTCAACCAATGGAAATTGGAAGAAGAACTGAGATTAGAAGAGGCACGAACAGTTGAGGAAGCTGCATTGTCACTTGTAGGGAAGGAGAAAGCAAAGTGTAGGGCTGCCATGGAGGCAGCAGAAGCAGCTCAAAGGATTGCTGAATTGGAAACACAAAAAAGGAGAAATGTAGAATTGAAAGCCCTTAGAGAAACTGAACACAGGAAGACGGCGCTAGAATCAAACGCATATGACCTCAGGTACAGGAAATACACAATTGAGGAGATTGAAACAGCAACAAATGATTTCTCAGCGGGTTATAAGATTGGGGAAGGAGGCTATGGGCCTGTATATAGAGGTGAGCTCGACCACACACCAGTGGCAATAAAAGTTCTACGCCCCGATGCAGCTCATGGAGAGGAGCAGTTCAAGAAAGAGGTTGAAGTATTGAGTTGCATCAGACATCCAAACATGGTTCTCCTTCTTGGAGCATGTCCAGAATTTGGTTGTCTGGTCTATGAGTACATGGCTAATGGGAGCTTAGAGGACCGTCTCTTCCGGCGAGGTAACACCCCAGTTATTCCTTGGCAGTTAAGGTTCAGAATTGCTGCAGAAATTGCGACAGGGCTTTTGTTCCTTCATCAGACCAAGCCAGAACCCCTTGTCCACCGTGACCTAAAACCTGGAAACATTTTGCTAGACCATAACTATGTGAGCAAGATCAGTGATGTAGGTTTGGCCAGGCTTGTTCCTGCATCGGTTGCCAATTCTGTGACTCAGTATCACATGACATCAACAGCCGGAACATTCTGTTATATCGACCCAGAGTATCAGCAAACAGGACTGCTTGGAGTAAAATCTGATGTCTATTCACTTGGGGTCCTGCTTCTACAAATAGTGACTGCCAGACCACCAATGGGTTTGACTCATCTTGTTGAGGAAGCTATTGACACAGGGGCTTTTGCTGAGGTGCTTGACCCTGCTATTTCTGACTGGCCTATTGAGGAAGCCTTGAAGTTTGCCAAGCTTTCTTTGCAGTGTGCTGAAATGAGGCGAAAAGACAGACCAGATCTCGGGAAGGTTCTTCTACCGGAGCTTAATAGATTCAGAGAATTTGGTCAAGATAGCATGAATCGGCTCATGTTTGGCGGTAGCCAAGTGTTCTCAGAAAAACGAGGCGCGATTTCCACAAGACAAGATGTGATAAGTGACACCAATCTAACACATTCAGGATATGATAGCTCAAGGAGTTAG

Protein Analysis

791

Amino Acids

88.31

Weight (kDa)

6.57

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
AccB1I GGYRCC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 1622
AccI GTMKAC 1 cut(s) 54
AccII CGCG 4 cut(s) 264, 814, 1028, 2300
AclWI GGATC 2 cut(s) 498, 771
AcoI YGGCCR 2 cut(s) 478, 1857
AcsI RAATTY 2 cut(s) 1619, 2231
AcyI GRCGYC 1 cut(s) 1041
AfaI GTAC 4 cut(s) 522, 1091, 1382, 1643
AfiI CCNNNNNNNGG 6 cut(s) 189, 263, 1050, 1622, 2036, 2192
AflIII ACRYGT 1 cut(s) 768
AgeI ACCGGT 1 cut(s) 512
AgsI TTSAA 7 cut(s) 822, 995, 1318, 1409, 1549, 1562, 2134
AhdI GACNNNNNGTC 1 cut(s) 793
AjnI CCWGG 4 cut(s) 19, 754, 1801, 1859
AleI CACNNNNGTG 1 cut(s) 1496
AloI GAACNNNNNNTCC 2 cut(s) 1767, 1799
Alw21I GWGCWC 5 cut(s) 216, 625, 899, 1071, 1485
Alw26I GTCTC 2 cut(s) 538, 1676
Alw44I GTGCAC 1 cut(s) 212
AlwI GGATC 2 cut(s) 498, 771
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 2192
AoxI GGCC 6 cut(s) 115, 201, 478, 1463, 1857, 2114
ApaLI GTGCAC 1 cut(s) 212
ApeKI GCWGC 8 cut(s) 744, 967, 1202, 1244, 1256, 1265, 1526, 1727
ApoI RAATTY 2 cut(s) 1619, 2231
ArsI GACNNNNNNTTYG 2 cut(s) 1732, 1764
AsiGI ACCGGT 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 2 cut(s) 1354, 2300
AspS9I GGNCC 4 cut(s) 115, 1463, 1666, 1999
AsuC2I CCSGG 2 cut(s) 184, 482
AsuHPI GGTGA 7 cut(s) 149, 167, 528, 912, 933, 954, 1490
AvaI CYCGRG 1 cut(s) 2192
AvaII GGWCC 2 cut(s) 1666, 1999
AxyI CCTNAGG 1 cut(s) 1376
BaeGI GKGCMC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 1859
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 3 cut(s) 899, 1071, 1485
BauI CACGAG 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 1352
Bbv12I GWGCWC 5 cut(s) 216, 625, 899, 1071, 1485
BbvCI CCTCAGC 1 cut(s) 2085
BbvI GCAGC 8 cut(s) 756, 979, 1189, 1231, 1268, 1277, 1538, 1714
BccI CCATC 2 cut(s) 180, 394
BceAI ACGGC 2 cut(s) 876, 1365
BciT130I CCWGG 4 cut(s) 21, 756, 1803, 1861
BcnI CCSGG 2 cut(s) 184, 482
BcoDI GTCTC 2 cut(s) 538, 1676
BfaI CTAG 3 cut(s) 1064, 1355, 1817
BfmI CTRYAG 1 cut(s) 1728
BfoI RGCGCY 1 cut(s) 1355
BglI GCCNNNNNGGC 1 cut(s) 1253
BglII AGATCT 1 cut(s) 2188
BisI GCNGC 9 cut(s) 265, 745, 968, 1203, 1245, 1257, 1266, 1527, 1728
BlsI GCNGC 9 cut(s) 266, 746, 969, 1204, 1246, 1258, 1267, 1528, 1729
Bme1390I CCNGG 6 cut(s) 21, 184, 482, 756, 1803, 1861
Bme18I GGWCC 2 cut(s) 1666, 1999
BmeRI GACNNNNNGTC 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 2192
BmgT120I GGNCC 4 cut(s) 115, 1463, 1666, 1999
BmiI GGNNCC 4 cut(s) 116, 450, 877, 2000
BmrFI CCNGG 6 cut(s) 21, 184, 482, 756, 1803, 1861
BmrI ACTGGG 1 cut(s) 1688
BmsI GCATC 9 cut(s) 161, 220, 568, 664, 872, 955, 1513, 1584, 1883
BmuI ACTGGG 1 cut(s) 1688
BpiI GAAGAC 1 cut(s) 1352
BplI GAGNNNNNCTC 4 cut(s) 548, 580, 1467, 1499
BpmI CTGGAG 1 cut(s) 550
Bpu10I CCTNAGC 2 cut(s) 1058, 2085
BpuEI CTTGAG 3 cut(s) 219, 531, 2347
BpuMI CCSGG 2 cut(s) 184, 482
BsaHI GRCGYC 1 cut(s) 1041
BsaJI CCNNGG 5 cut(s) 262, 417, 481, 1248, 1703
BsaWI WCCGGW 3 cut(s) 512, 718, 2209
Bsc4I CCNNNNNNNGG 6 cut(s) 189, 263, 1050, 1622, 2036, 2192
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 1496, 1694, 2117
Bse21I CCTNAGG 1 cut(s) 1376
Bse3DI GCAATG 1 cut(s) 128
BseBI CCWGG 4 cut(s) 21, 756, 1803, 1861
BseDI CCNNGG 5 cut(s) 262, 417, 481, 1248, 1703
BseGI GGATG 6 cut(s) 427, 606, 655, 863, 1036, 1582
BseLI CCNNNNNNNGG 6 cut(s) 189, 263, 1050, 1622, 2036, 2192
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 8 cut(s) 121, 1072, 1160, 1390, 1443, 1910, 2076, 2298
BseNI ACTGG 3 cut(s) 1496, 1694, 2117
BseRI GAGGAG 4 cut(s) 212, 1066, 1415, 1553
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 8 cut(s) 756, 979, 1189, 1231, 1268, 1277, 1538, 1714
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 4 cut(s) 264, 814, 1028, 2300
BshFI GGCC 6 cut(s) 117, 203, 480, 1465, 1859, 2116
BshNI GGYRCC 1 cut(s) 448
BshTI ACCGGT 1 cut(s) 512
BsiHKAI GWGCWC 5 cut(s) 216, 625, 899, 1071, 1485
BsiHKCI CYCGRG 1 cut(s) 2192
BsiSI CCGG 8 cut(s) 183, 204, 481, 513, 719, 1678, 1920, 2210
BslFI GGGAC 2 cut(s) 18, 205
BslI CCNNNNNNNGG 6 cut(s) 189, 263, 1050, 1622, 2036, 2192
BsmAI GTCTC 2 cut(s) 538, 1676
BsmBI CGTCTC 1 cut(s) 1676
BsmFI GGGAC 2 cut(s) 18, 205
BsnI GGCC 6 cut(s) 117, 203, 480, 1465, 1859, 2116
BsoBI CYCGRG 1 cut(s) 2192
Bsp1286I GDGCHC 5 cut(s) 216, 625, 899, 1071, 1485
Bsp1407I TGTACA 1 cut(s) 1089
Bsp143I GATC 5 cut(s) 300, 503, 763, 1839, 2188
Bsp19I CCATGG 1 cut(s) 1248
BspANI GGCC 6 cut(s) 117, 203, 480, 1465, 1859, 2116
BspCNI CTCAG 8 cut(s) 120, 1071, 1161, 1389, 1442, 1909, 2077, 2297
BspFNI CGCG 4 cut(s) 264, 814, 1028, 2300
BspHI TCATGA 2 cut(s) 411, 949
BspLI GGNNCC 4 cut(s) 116, 450, 877, 2000
BspMAI CTGCAG 1 cut(s) 1732
BspPI GGATC 2 cut(s) 498, 771
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 1089
BsrI ACTGG 3 cut(s) 1496, 1694, 2117
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 5 cut(s) 262, 417, 481, 1248, 1703
BssMI GATC 5 cut(s) 300, 503, 763, 1839, 2188
BssNI GRCGYC 1 cut(s) 1041
BssSI CACGAG 1 cut(s) 1006
BssT1I CCWWGG 2 cut(s) 1248, 1703
Bst2BI CACGAG 1 cut(s) 1006
Bst2UI CCWGG 4 cut(s) 21, 756, 1803, 1861
Bst4CI ACNGT 7 cut(s) 94, 466, 639, 725, 1192, 1670, 1790
Bst6I CTCTTC 3 cut(s) 27, 1173, 1679
BstACI GRCGYC 1 cut(s) 1041
BstAPI GCANNNNNTGC 2 cut(s) 1736, 1880
BstAUI TGTACA 1 cut(s) 1089
BstC8I GCNNGC 2 cut(s) 78, 1102
BstDSI CCRYGG 3 cut(s) 262, 417, 1248
BstF5I GGATG 6 cut(s) 427, 606, 655, 863, 1036, 1582
BstFNI CGCG 4 cut(s) 264, 814, 1028, 2300
BstH2I RGCGCY 1 cut(s) 1355
BstHHI GCGC 2 cut(s) 1354, 2300
BstKTI GATC 5 cut(s) 303, 506, 766, 1842, 2191
BstMAI GTCTC 2 cut(s) 538, 1676
BstMBI GATC 5 cut(s) 300, 503, 763, 1839, 2188
BstMWI GCNNNNNNNGC 7 cut(s) 270, 1253, 1262, 1265, 1736, 1880, 2152
BstNI CCWGG 4 cut(s) 21, 756, 1803, 1861
BstNSI RCATGY 4 cut(s) 80, 772, 796, 1614
BstSCI CCNGG 6 cut(s) 19, 182, 480, 754, 1801, 1859
BstSFI CTRYAG 1 cut(s) 1728
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 4 cut(s) 264, 814, 1028, 2300
BstV1I GCAGC 8 cut(s) 756, 979, 1189, 1231, 1268, 1277, 1538, 1714
BstV2I GAAGAC 1 cut(s) 1352
BstX2I RGATCY 1 cut(s) 2188
BstYI RGATCY 1 cut(s) 2188
Bsu36I CCTNAGG 1 cut(s) 1376
BsuRI GGCC 6 cut(s) 117, 203, 480, 1465, 1859, 2116
BtgI CCRYGG 3 cut(s) 262, 417, 1248
BtsCI GGATG 6 cut(s) 427, 606, 655, 863, 1036, 1582
BtsI GCAGTG 1 cut(s) 2162
BtsIMutI CAGTG 5 cut(s) 692, 730, 1503, 1849, 2162
Cac8I GCNNGC 2 cut(s) 78, 1102
CaiI CAGNNNCTG 1 cut(s) 524
CciI TCATGA 2 cut(s) 411, 949
CfoI GCGC 2 cut(s) 1354, 2300
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 4 cut(s) 115, 1463, 1666, 1999
Cfr42I CCGCGG 1 cut(s) 265
CseI GACGC 1 cut(s) 1049
Csp6I GTAC 4 cut(s) 521, 1090, 1381, 1642
CspAI ACCGGT 1 cut(s) 512
CviQI GTAC 4 cut(s) 521, 1090, 1381, 1642
DpnI GATC 5 cut(s) 302, 505, 765, 1841, 2190
DpnII GATC 5 cut(s) 300, 503, 763, 1839, 2188
DriI GACNNNNNGTC 1 cut(s) 793
EaeI YGGCCR 2 cut(s) 478, 1857
Eam1104I CTCTTC 3 cut(s) 27, 1173, 1679
Eam1105I GACNNNNNGTC 1 cut(s) 793
EarI CTCTTC 3 cut(s) 27, 1173, 1679
Ecl136II GAGCTC 3 cut(s) 897, 1069, 1483
Eco130I CCWWGG 2 cut(s) 1248, 1703
Eco24I GRGCYC 3 cut(s) 899, 1071, 1485
Eco47I GGWCC 2 cut(s) 1666, 1999
Eco53kI GAGCTC 3 cut(s) 897, 1069, 1483
Eco81I CCTNAGG 1 cut(s) 1376
Eco88I CYCGRG 1 cut(s) 2192
EcoICRI GAGCTC 3 cut(s) 897, 1069, 1483
EcoO109I RGGNCCY 1 cut(s) 1999
EcoRII CCWGG 4 cut(s) 19, 754, 1801, 1859
EcoT14I CCWWGG 2 cut(s) 1248, 1703
EcoT38I GRGCYC 3 cut(s) 899, 1071, 1485
ErhI CCWWGG 2 cut(s) 1248, 1703
Esp3I CGTCTC 1 cut(s) 1676
FaqI GGGAC 2 cut(s) 18, 205
FauI CCCGC 1 cut(s) 1426
FauNDI CATATG 2 cut(s) 706, 1369
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 9 cut(s) 265, 745, 968, 1203, 1245, 1257, 1266, 1527, 1728
FokI GGATG 6 cut(s) 434, 613, 642, 850, 1043, 1569
FriOI GRGCYC 3 cut(s) 899, 1071, 1485
Fsp4HI GCNGC 9 cut(s) 265, 745, 968, 1203, 1245, 1257, 1266, 1527, 1728
FspBI CTAG 3 cut(s) 1064, 1355, 1817
GlaI GCGC 2 cut(s) 1353, 2299
GluI GCNGC 9 cut(s) 265, 745, 968, 1203, 1245, 1257, 1266, 1527, 1728
GsuI CTGGAG 1 cut(s) 550
HaeII RGCGCY 1 cut(s) 1355
HaeIII GGCC 6 cut(s) 117, 203, 480, 1465, 1859, 2116
HapII CCGG 8 cut(s) 183, 204, 481, 513, 719, 1678, 1920, 2210
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 2 cut(s) 1354, 2300
Hin1I GRCGYC 1 cut(s) 1041
Hin6I GCGC 2 cut(s) 1352, 2298
HinP1I GCGC 2 cut(s) 1352, 2298
HincII GTYRAC 2 cut(s) 340, 1144
HindII GTYRAC 2 cut(s) 340, 1144
HindIII AAGCTT 2 cut(s) 573, 2144
HinfI GANTC 9 cut(s) 35, 531, 581, 1113, 1358, 1894, 2044, 2223, 2251
HpaII CCGG 8 cut(s) 183, 204, 481, 513, 719, 1678, 1920, 2210
HphI GGTGA 7 cut(s) 149, 167, 528, 912, 933, 954, 1490
Hpy166II GTNNAC 6 cut(s) 55, 214, 340, 1039, 1144, 1786
Hpy8I GTNNAC 6 cut(s) 55, 214, 340, 1039, 1144, 1786
HpyAV CCTTC 7 cut(s) 22, 786, 1216, 1445, 1610, 1766, 2191
HpyCH4III ACNGT 7 cut(s) 94, 466, 639, 725, 1192, 1670, 1790
HpyF10VI GCNNNNNNNGC 7 cut(s) 270, 1253, 1262, 1265, 1736, 1880, 2152
Hsp92I GRCGYC 1 cut(s) 1041
HspAI GCGC 2 cut(s) 1352, 2298
KspI CCGCGG 1 cut(s) 265
Kzo9I GATC 5 cut(s) 300, 503, 763, 1839, 2188
LmnI GCTCC 6 cut(s) 138, 854, 1540, 1607, 1656, 2212
Lsp1109I GCAGC 8 cut(s) 756, 979, 1189, 1231, 1268, 1277, 1538, 1714
LweI GCATC 9 cut(s) 161, 220, 568, 664, 872, 955, 1513, 1584, 1883
MaeI CTAG 3 cut(s) 1064, 1355, 1817
MalI GATC 5 cut(s) 302, 505, 765, 1841, 2190
MboI GATC 5 cut(s) 300, 503, 763, 1839, 2188
MboII GAAGA 9 cut(s) 44, 95, 245, 1172, 1175, 1190, 1357, 1666, 2195
MfeI CAATTG 2 cut(s) 495, 1395
MflI RGATCY 1 cut(s) 2188
MhlI GDGCHC 5 cut(s) 216, 625, 899, 1071, 1485
MlsI TGGCCA 1 cut(s) 1859
MluNI TGGCCA 1 cut(s) 1859
MlyI GAGTC 5 cut(s) 44, 540, 1122, 1888, 2038
MmeI TCCRAC 1 cut(s) 452
Mox20I TGGCCA 1 cut(s) 1859
MroXI GAANNNNTTC 1 cut(s) 249
MscI TGGCCA 1 cut(s) 1859
MseI TTAA 3 cut(s) 359, 1712, 2217
MslI CAYNNNNRTG 2 cut(s) 1496, 1827
Msp20I TGGCCA 1 cut(s) 1859
MspA1I CMGCKG 3 cut(s) 264, 747, 1433
MspI CCGG 8 cut(s) 183, 204, 481, 513, 719, 1678, 1920, 2210
MspR9I CCNGG 6 cut(s) 21, 184, 482, 756, 1803, 1861
MunI CAATTG 2 cut(s) 495, 1395
MvaI CCWGG 4 cut(s) 21, 756, 1803, 1861
MvnI CGCG 4 cut(s) 264, 814, 1028, 2300
MwoI GCNNNNNNNGC 7 cut(s) 270, 1253, 1262, 1265, 1736, 1880, 2152
NciI CCSGG 2 cut(s) 184, 482
NcoI CCATGG 1 cut(s) 1248
NdeI CATATG 2 cut(s) 706, 1369
NdeII GATC 5 cut(s) 300, 503, 763, 1839, 2188
NlaIV GGNNCC 4 cut(s) 116, 450, 877, 2000
NmeAIII GCCGAG 1 cut(s) 1069
NmuCI GTSAC 8 cut(s) 124, 1017, 1114, 1209, 1790, 1891, 2017, 2327
NspI RCATGY 4 cut(s) 80, 772, 796, 1614
OliI CACNNNNGTG 1 cut(s) 1496
PaeI GCATGC 1 cut(s) 80
PagI TCATGA 2 cut(s) 411, 949
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 1013
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 4 cut(s) 581, 1358, 2223, 2251
PflMI CCANNNNNTGG 1 cut(s) 1622
PinAI ACCGGT 1 cut(s) 512
PkrI GCNGC 9 cut(s) 266, 746, 969, 1204, 1246, 1258, 1267, 1528, 1729
PleI GAGTC 5 cut(s) 43, 539, 1121, 1888, 2038
PpsI GAGTC 5 cut(s) 43, 539, 1121, 1888, 2038
PpuMI RGGWCCY 1 cut(s) 1999
PscI ACATGT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 1440
Psp124BI GAGCTC 3 cut(s) 899, 1071, 1485
Psp5II RGGWCCY 1 cut(s) 1999
Psp6I CCWGG 4 cut(s) 19, 754, 1801, 1859
PspGI CCWGG 4 cut(s) 19, 754, 1801, 1859
PspN4I GGNNCC 4 cut(s) 116, 450, 877, 2000
PspPI GGNCC 4 cut(s) 115, 1463, 1666, 1999
PspPPI RGGWCCY 1 cut(s) 1999
PstI CTGCAG 1 cut(s) 1732
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 1 cut(s) 2188
RsaI GTAC 4 cut(s) 522, 1091, 1382, 1643
RsaNI GTAC 4 cut(s) 521, 1090, 1381, 1642
RseI CAYNNNNRTG 2 cut(s) 1496, 1827
SacI GAGCTC 3 cut(s) 899, 1071, 1485
SacII CCGCGG 1 cut(s) 265
SaqAI TTAA 3 cut(s) 359, 1712, 2217
SatI GCNGC 9 cut(s) 265, 745, 968, 1203, 1245, 1257, 1266, 1527, 1728
Sau3AI GATC 5 cut(s) 300, 503, 763, 1839, 2188
Sau96I GGNCC 4 cut(s) 115, 1463, 1666, 1999
SchI GAGTC 5 cut(s) 44, 540, 1122, 1888, 2038
ScrFI CCNGG 6 cut(s) 21, 184, 482, 756, 1803, 1861
SduI GDGCHC 5 cut(s) 216, 625, 899, 1071, 1485
SfaNI GCATC 9 cut(s) 161, 220, 568, 664, 872, 955, 1513, 1584, 1883
SfcI CTRYAG 1 cut(s) 1728
Sfr303I CCGCGG 1 cut(s) 265
SgrBI CCGCGG 1 cut(s) 265
SinI GGWCC 2 cut(s) 1666, 1999
SmiMI CAYNNNNRTG 2 cut(s) 1496, 1827
SmlI CTYRAG 3 cut(s) 234, 546, 2362
SmoI CTYRAG 3 cut(s) 234, 546, 2362
SphI GCATGC 1 cut(s) 80
SspMI CTAG 3 cut(s) 1064, 1355, 1817
SstI GAGCTC 3 cut(s) 899, 1071, 1485
StyD4I CCNGG 6 cut(s) 19, 182, 480, 754, 1801, 1859
StyI CCWWGG 2 cut(s) 1248, 1703
TaaI ACNGT 7 cut(s) 94, 466, 639, 725, 1192, 1670, 1790
TaqI TCGA 4 cut(s) 440, 888, 1485, 1938
TatI WGTACW 2 cut(s) 1089, 1641
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 4 cut(s) 581, 1358, 2223, 2251
Tru1I TTAA 3 cut(s) 359, 1712, 2217
Tru9I TTAA 3 cut(s) 359, 1712, 2217
TscAI CASTG 5 cut(s) 699, 730, 1503, 1849, 2162
TseFI GTSAC 8 cut(s) 124, 1017, 1114, 1209, 1790, 1891, 2017, 2327
TseI GCWGC 8 cut(s) 744, 967, 1202, 1244, 1256, 1265, 1526, 1727
Tsp45I GTSAC 8 cut(s) 124, 1017, 1114, 1209, 1790, 1891, 2017, 2327
TspDTI ATGAA 4 cut(s) 400, 768, 1748, 2264
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 5 cut(s) 699, 730, 1503, 1849, 2162
Van91I CCANNNNNTGG 1 cut(s) 1622
VneI GTGCAC 1 cut(s) 212
VpaK11BI GGWCC 2 cut(s) 1666, 1999
XapI RAATTY 2 cut(s) 1619, 2231
XceI RCATGY 4 cut(s) 80, 772, 796, 1614
XcmI CCANNNNNNNNNTGG 3 cut(s) 185, 1701, 2032
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 3 cut(s) 1064, 1355, 1817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.