MD17G1212900.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000353
Physical Location & Seq
Reverse (-)
26008786 .. 26013080
4295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1212900.v1.1.491

Sequence Viewer

Length: 1908 bp
ATGGGGATGAGAACATCGAGAAGGGAAGGTTCAGAGAAGAGAGAGGAGGCAGTGGCATTGGCAATAGACAAGGATAAAGCAAGTGAGTATGCAATCAAATGGATTGCTGATCATCTTTTTACCAAGCCTGGACAACCTCTCACTTTGCTCCATGTTCATGTTAAACAACCTTCATCTCCCCTAATTTCCACCCAAAGAGGGGAATTGACAGAGGAGAACAATTACCAAGTTGAAGATCAAGCCAAAGATTTGTTCCTCCCATTCCGTTGCTTCTGCACTAAAAAAGCTATCAAAAGCAACGAAGTTATAGTGGAGGATACTGATATAGCAAAAGCACTTATCAACTATGTCGCTGCTAATTCTATTCGGGTTTTGGTGCTTGGCGCACCATCCAAAAACAGCTTTGGCTTTTTCAGATTCAAAGCGAAAGACATCCCAAGCACCGTGTCCAAAGGGGCACCAGATTTTTGCACTCTGTATGTCATTGGCAAAGGAAAGATCACGCATTGCCGACCAGCTACTGCTGAACTACCCTCAAATGATTCGAGAAACTCGCAACACATGAGGCTCCACCAGCACTCCAGAGGTTTGTTTCTAACCGGAGAGCCCGCTGAAGAACAAGTTCAGGATGACATGGAAGCTGAGATGAGGAGGCTCAGGCTAGAGCTCAAGCAAACAATGGAGATGTACAGCATGGCTTGCAAGGAGGCAATCTCAGCAAAACATAGGGAAAAGGAACTTCACCAGTGGAAATTGAACGGAGAGCACAGATTAGAAGAAGCACGACTCGCTGAGGGAGCTGCATTTGCACTTGCACAGAAGGAGAAAGCGAAAAGTAAGGCAGCAATTGAGGCAGCACAAGCGGCTGAAAGGGTTGCAGAACTGGAAGCAGAAAGACGGAAAAACTTAGAAATGAAAGCTCTTGAAAAAGCTAAAGGAAAGAAGTCGCTAGCATCAAATGGGTACGATCTCAGGTACAGGAGATACACAATTGAAGATATTGAAGCGGCGACGGAGTATTTTTCACAAGCCTATAAGATTGGAGAAGGAGGATACGGTCCAGTCTACAGAGGTGAACTAGATCACACACCGGTAGCAATAAAAGTTCTGCGTCCGGATGCAGCCCAAGGGCGGTCGCAATTCCAGCAAGAGGTTGAAGTATTGAGCTGCATTAGGCATCCAAACATGGTTCTCCTACTTGGAGCCTGCCCAGAATTTGGTTGTCTGGTCTATGAGTACATGGCCAATGGTAGCTTAGAAGACCGTCTCTTCCGGAAAGGCAACACCCCGGTGATTCCTTGGCAGCTAAGGTTCAGAATTGCTGCAGAAATTGCCACCGGACTTTTGTTTCTTCATCAGACCAAGCCAGAACCCGTTGTACACCGTGACCTAAAACCCGGCAACATCTTGCTAGACCATAACTATGTGAGTAAGATTAGTGACGTAGGTTTGGCCAGGCTTGTCCCTCCATCAGTAGCCAATGCTGTCACTCAATACCACGTGACATCAATGGCCGGAACATTCTGTTATATCGACCCAGAGTACCAGCAGACCGGAATGCTTGGGGTGAAATCTGACGTTTACTCACTCGGGGTCATGCTTCTACAACTAATAACTGCCCAGCCGCCAATGGGTTTGACTTTCAAAGTTGAAGATGCTATTGAGAGTGGGACTTTCGCGGAGATGCTTGACCCTGATGTTCCCGACTGGCCTGTTGAGGAAGCATTAAAGTTTGCGCAGCTAGCCTTACAGTGCGCTGAAATGAGGAGAAAAGACAGACCGGATCTTGGTAAGGTTGTGCTACCGGAGCTAAACAGATTGAGAGTACTCGCGGACGAGAGAATGGATCCTTTCAGGCTAGGTCGTAGTGCACCATACTCACCAAGACAAGTAAGTTACATAACATAA

Protein Analysis

636

Amino Acids

71.4

Weight (kDa)

7.59

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 18 - 136 3.6e-09 Universal stress protein family
Pkinase PF00069 343 - 595 2.3e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 345 - 599 4.3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1737
AccB1I GGYRCC 1 cut(s) 457
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 2 cut(s) 1679, 1832
AccIII TCCGGA 2 cut(s) 1114, 1272
AciI CCGC 7 cut(s) 609, 863, 1007, 1132, 1625, 1679, 1832
AclWI GGATC 3 cut(s) 1791, 1841, 1854
AcoI YGGCCR 3 cut(s) 1242, 1452, 1512
AcsI RAATTY 1 cut(s) 1214
AcuI CTGAAG 1 cut(s) 633
AcvI CACGTG 1 cut(s) 1501
AfaI GTAC 7 cut(s) 689, 965, 977, 1238, 1380, 1544, 1827
AfiI CCNNNNNNNGG 7 cut(s) 198, 199, 1131, 1150, 1217, 1631, 1787
AgeI ACCGGT 1 cut(s) 1090
AgsI TTSAA 9 cut(s) 233, 421, 757, 926, 995, 1004, 1157, 1645, 1652
AjnI CCWGG 2 cut(s) 127, 1454
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AleI CACNNNNGTG 1 cut(s) 1289
Alw21I GWGCWC 3 cut(s) 669, 768, 1873
Alw26I GTCTC 1 cut(s) 1271
Alw44I GTGCAC 1 cut(s) 1869
AlwI GGATC 3 cut(s) 1791, 1841, 1854
AlwNI CAGNNNCTG 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 1589
Aor13HI TCCGGA 2 cut(s) 1114, 1272
AoxI GGCC 4 cut(s) 1242, 1452, 1512, 1709
ApaLI GTGCAC 1 cut(s) 1869
ApeKI GCWGC 9 cut(s) 353, 800, 842, 854, 1121, 1167, 1303, 1322, 1738
ApoI RAATTY 1 cut(s) 1214
AsiGI ACCGGT 1 cut(s) 1090
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 3 cut(s) 386, 1738, 1757
AspS9I GGNCC 1 cut(s) 1058
AsuC2I CCSGG 2 cut(s) 1289, 1398
AsuHPI GGTGA 5 cut(s) 734, 1085, 1303, 1579, 1872
AsuNHI GCTAGC 2 cut(s) 949, 1741
AvaI CYCGRG 1 cut(s) 1589
AvaII GGWCC 1 cut(s) 1058
BaeGI GKGCMC 2 cut(s) 460, 1873
BalI TGGCCA 2 cut(s) 1244, 1454
BamHI GGATCC 1 cut(s) 1846
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 2 cut(s) 609, 669
BbrPI CACGTG 1 cut(s) 1501
BbsI GAAGAC 1 cut(s) 1266
Bbv12I GWGCWC 3 cut(s) 669, 768, 1873
BbvCI CCTCAGC 1 cut(s) 792
BbvI GCAGC 9 cut(s) 340, 787, 854, 866, 1133, 1154, 1309, 1315, 1750
BccI CCATC 2 cut(s) 397, 1477
BciT130I CCWGG 2 cut(s) 129, 1456
BciVI GTATCC 2 cut(s) 310, 1046
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 2 cut(s) 1289, 1398
BcoDI GTCTC 1 cut(s) 1271
BfaI CTAG 6 cut(s) 662, 950, 1079, 1412, 1742, 1859
BfmI CTRYAG 2 cut(s) 1066, 1323
BfuI GTATCC 2 cut(s) 310, 1046
BmcAI AGTACT 1 cut(s) 1827
Bme1390I CCNGG 4 cut(s) 129, 1289, 1398, 1456
Bme18I GGWCC 1 cut(s) 1058
BmeT110I CYCGRG 1 cut(s) 1589
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 4 cut(s) 459, 569, 1204, 1848
BmrFI CCNGG 4 cut(s) 129, 1289, 1398, 1456
BmsI GCATC 5 cut(s) 962, 1108, 1186, 1645, 1674
BmtI GCTAGC 2 cut(s) 953, 1745
BpiI GAAGAC 1 cut(s) 1266
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 1 cut(s) 565
Bpu10I CCTNAGC 3 cut(s) 656, 792, 1307
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 1289, 1398
BsaAI YACGTR 1 cut(s) 1501
BsaJI CCNNGG 3 cut(s) 1126, 1287, 1298
BsaWI WCCGGW 8 cut(s) 599, 1090, 1114, 1272, 1337, 1553, 1780, 1804
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 7 cut(s) 198, 199, 1131, 1150, 1217, 1631, 1787
Bse118I RCCGGY 1 cut(s) 1090
Bse1I ACTGG 4 cut(s) 745, 888, 1061, 1712
Bse3DI GCAATG 1 cut(s) 505
BseAI TCCGGA 2 cut(s) 1114, 1272
BseBI CCWGG 2 cut(s) 129, 1456
BseDI CCNNGG 3 cut(s) 1126, 1287, 1298
BseGI GGATG 6 cut(s) 12, 389, 432, 634, 1123, 1177
BseLI CCNNNNNNNGG 7 cut(s) 198, 199, 1131, 1150, 1217, 1631, 1787
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 5 cut(s) 633, 670, 729, 783, 985
BseNI ACTGG 4 cut(s) 745, 888, 1061, 1712
BseRI GAGGAG 4 cut(s) 59, 227, 664, 1780
BseSI GKGCMC 2 cut(s) 460, 1873
BseXI GCAGC 9 cut(s) 340, 787, 854, 866, 1133, 1154, 1309, 1315, 1750
BseYI CCCAGC 1 cut(s) 1620
BsgI GTGCAG 1 cut(s) 259
Bsh1236I CGCG 2 cut(s) 1679, 1832
Bsh1285I CGRYCG 1 cut(s) 1136
BshFI GGCC 4 cut(s) 1244, 1454, 1514, 1711
BshNI GGYRCC 1 cut(s) 457
BshTI ACCGGT 1 cut(s) 1090
BsiEI CGRYCG 1 cut(s) 1136
BsiHKAI GWGCWC 3 cut(s) 669, 768, 1873
BsiHKCI CYCGRG 1 cut(s) 1589
BslFI GGGAC 2 cut(s) 1448, 1684
BslI CCNNNNNNNGG 7 cut(s) 198, 199, 1131, 1150, 1217, 1631, 1787
BsmAI GTCTC 1 cut(s) 1271
BsmBI CGTCTC 1 cut(s) 1271
BsmFI GGGAC 2 cut(s) 1448, 1684
BsmI GAATGC 1 cut(s) 1563
BsnI GGCC 4 cut(s) 1244, 1454, 1514, 1711
BsoBI CYCGRG 1 cut(s) 1589
Bsp1286I GDGCHC 5 cut(s) 460, 609, 669, 768, 1873
Bsp13I TCCGGA 2 cut(s) 1114, 1272
Bsp1407I TGTACA 2 cut(s) 687, 1378
Bsp143I GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
BspACI CCGC 7 cut(s) 609, 863, 1007, 1132, 1625, 1679, 1832
BspANI GGCC 4 cut(s) 1244, 1454, 1514, 1711
BspCNI CTCAG 5 cut(s) 634, 669, 728, 784, 984
BspEI TCCGGA 2 cut(s) 1114, 1272
BspFNI CGCG 2 cut(s) 1679, 1832
BspLI GGNNCC 4 cut(s) 459, 569, 1204, 1848
BspMAI CTGCAG 1 cut(s) 1327
BspOI GCTAGC 2 cut(s) 953, 1745
BspPI GGATC 3 cut(s) 1791, 1841, 1854
BspT107I GGYRCC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 1090
BsrGI TGTACA 2 cut(s) 687, 1378
BsrI ACTGG 4 cut(s) 745, 888, 1061, 1712
BssAI RCCGGY 1 cut(s) 1090
BssECI CCNNGG 3 cut(s) 1126, 1287, 1298
BssMI GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
BssT1I CCWWGG 2 cut(s) 1126, 1298
Bst2UI CCWGG 2 cut(s) 129, 1456
Bst4CI ACNGT 5 cut(s) 445, 1058, 1265, 1385, 1752
Bst6I CTCTTC 2 cut(s) 32, 1274
BstAPI GCANNNNNTGC 2 cut(s) 699, 1331
BstAUI TGTACA 2 cut(s) 687, 1378
BstBAI YACGTR 1 cut(s) 1501
BstC8I GCNNGC 5 cut(s) 609, 700, 951, 1207, 1743
BstDEI CTNAG 8 cut(s) 642, 656, 715, 792, 907, 971, 1255, 1307
BstF5I GGATG 6 cut(s) 12, 389, 432, 634, 1123, 1177
BstFNI CGCG 2 cut(s) 1679, 1832
BstHHI GCGC 3 cut(s) 386, 1738, 1757
BstKTI GATC 7 cut(s) 112, 238, 501, 970, 1084, 1786, 1849
BstMAI GTCTC 1 cut(s) 1271
BstMBI GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
BstMCI CGRYCG 1 cut(s) 1136
BstNI CCWGG 2 cut(s) 129, 1456
BstSCI CCNGG 4 cut(s) 127, 1287, 1396, 1454
BstSFI CTRYAG 2 cut(s) 1066, 1323
BstSLI GKGCMC 2 cut(s) 460, 1873
BstUI CGCG 2 cut(s) 1679, 1832
BstV1I GCAGC 9 cut(s) 340, 787, 854, 866, 1133, 1154, 1309, 1315, 1750
BstV2I GAAGAC 1 cut(s) 1266
BstX2I RGATCY 2 cut(s) 1783, 1846
BstYI RGATCY 2 cut(s) 1783, 1846
BsuI GTATCC 2 cut(s) 310, 1046
BsuRI GGCC 4 cut(s) 1244, 1454, 1514, 1711
BtsCI GGATG 6 cut(s) 12, 389, 432, 634, 1123, 1177
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 3 cut(s) 57, 752, 1757
Cac8I GCNNGC 5 cut(s) 609, 700, 951, 1207, 1743
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 3 cut(s) 386, 1738, 1757
Cfr10I RCCGGY 1 cut(s) 1090
Cfr13I GGNCC 1 cut(s) 1058
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 7 cut(s) 688, 964, 976, 1237, 1379, 1543, 1826
CspAI ACCGGT 1 cut(s) 1090
CviAII CATG 8 cut(s) 152, 158, 562, 634, 694, 1186, 1240, 1597
CviQI GTAC 7 cut(s) 688, 964, 976, 1237, 1379, 1543, 1826
DdeI CTNAG 8 cut(s) 642, 656, 715, 792, 907, 971, 1255, 1307
DpnI GATC 7 cut(s) 111, 237, 500, 969, 1083, 1785, 1848
DpnII GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
EaeI YGGCCR 3 cut(s) 1242, 1452, 1512
Eam1104I CTCTTC 2 cut(s) 32, 1274
EarI CTCTTC 2 cut(s) 32, 1274
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 2 cut(s) 1126, 1298
Eco24I GRGCYC 2 cut(s) 609, 669
Eco47I GGWCC 1 cut(s) 1058
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 633
Eco72I CACGTG 1 cut(s) 1501
Eco88I CYCGRG 1 cut(s) 1589
EcoICRI GAGCTC 1 cut(s) 667
EcoRII CCWGG 2 cut(s) 127, 1454
EcoT14I CCWWGG 2 cut(s) 1126, 1298
EcoT38I GRGCYC 2 cut(s) 609, 669
ErhI CCWWGG 2 cut(s) 1126, 1298
Esp3I CGTCTC 1 cut(s) 1271
FaeI CATG 8 cut(s) 155, 161, 565, 637, 697, 1189, 1243, 1600
FaqI GGGAC 2 cut(s) 1448, 1684
FatI CATG 8 cut(s) 151, 157, 561, 633, 693, 1185, 1239, 1596
FauI CCCGC 1 cut(s) 616
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 1 cut(s) 1065
FokI GGATG 6 cut(s) 19, 376, 419, 641, 1130, 1164
FriOI GRGCYC 2 cut(s) 609, 669
FspBI CTAG 6 cut(s) 662, 950, 1079, 1412, 1742, 1859
FspI TGCGCA 1 cut(s) 1737
GlaI GCGC 3 cut(s) 385, 1737, 1756
GsaI CCCAGC 1 cut(s) 1624
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 4 cut(s) 1244, 1454, 1514, 1711
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 3 cut(s) 386, 1738, 1757
Hin1II CATG 8 cut(s) 155, 161, 565, 637, 697, 1189, 1243, 1600
Hin6I GCGC 3 cut(s) 384, 1736, 1755
HinP1I GCGC 3 cut(s) 384, 1736, 1755
HinfI GANTC 4 cut(s) 417, 542, 786, 1294
HphI GGTGA 5 cut(s) 734, 1085, 1303, 1579, 1872
Hpy166II GTNNAC 5 cut(s) 1066, 1076, 1381, 1582, 1871
Hpy188I TCNGA 5 cut(s) 34, 416, 1316, 1359, 1576
Hpy188III TCNNGA 8 cut(s) 18, 546, 582, 626, 923, 1115, 1273, 1703
Hpy8I GTNNAC 5 cut(s) 1066, 1076, 1381, 1582, 1871
Hpy99I CGWCG 1 cut(s) 1015
HpyAV CCTTC 5 cut(s) 15, 20, 180, 814, 1040
HpyCH4III ACNGT 5 cut(s) 445, 1058, 1265, 1385, 1752
HpyCH4IV ACGT 3 cut(s) 1443, 1500, 1578
HpyF3I CTNAG 8 cut(s) 642, 656, 715, 792, 907, 971, 1255, 1307
HpySE526I ACGT 3 cut(s) 1443, 1500, 1578
Hsp92II CATG 8 cut(s) 155, 161, 565, 637, 697, 1189, 1243, 1600
HspAI GCGC 3 cut(s) 384, 1736, 1755
Kpn2I TCCGGA 2 cut(s) 1114, 1272
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
LmnI GCTCC 5 cut(s) 153, 573, 797, 1202, 1807
Lsp1109I GCAGC 9 cut(s) 340, 787, 854, 866, 1133, 1154, 1309, 1315, 1750
LweI GCATC 5 cut(s) 962, 1108, 1186, 1645, 1674
MaeI CTAG 6 cut(s) 662, 950, 1079, 1412, 1742, 1859
MaeII ACGT 3 cut(s) 1443, 1500, 1578
MaeIII GTNAC 5 cut(s) 1385, 1439, 1486, 1501, 1895
MalI GATC 7 cut(s) 111, 237, 500, 969, 1083, 1785, 1848
MboI GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
MboII GAAGA 9 cut(s) 49, 245, 626, 788, 1007, 1261, 1271, 1343, 1664
MfeI CAATTG 2 cut(s) 846, 990
MflI RGATCY 2 cut(s) 1783, 1846
MhlI GDGCHC 5 cut(s) 460, 609, 669, 768, 1873
MlsI TGGCCA 2 cut(s) 1244, 1454
MluNI TGGCCA 2 cut(s) 1244, 1454
MlyI GAGTC 1 cut(s) 780
Mox20I TGGCCA 2 cut(s) 1244, 1454
MroI TCCGGA 2 cut(s) 1114, 1272
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 2 cut(s) 1244, 1454
MseI TTAA 2 cut(s) 162, 1727
MslI CAYNNNNRTG 3 cut(s) 156, 1289, 1422
Msp20I TGGCCA 2 cut(s) 1244, 1454
MspA1I CMGCKG 1 cut(s) 611
MspR9I CCNGG 4 cut(s) 129, 1289, 1398, 1456
MunI CAATTG 2 cut(s) 846, 990
Mva1269I GAATGC 1 cut(s) 1563
MvaI CCWGG 2 cut(s) 129, 1456
MvnI CGCG 2 cut(s) 1679, 1832
NciI CCSGG 2 cut(s) 1289, 1398
NdeII GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
NheI GCTAGC 2 cut(s) 949, 1741
NlaIII CATG 8 cut(s) 155, 161, 565, 637, 697, 1189, 1243, 1600
NlaIV GGNNCC 4 cut(s) 459, 569, 1204, 1848
NmuCI GTSAC 4 cut(s) 1385, 1439, 1486, 1501
NsbI TGCGCA 1 cut(s) 1737
OliI CACNNNNGTG 1 cut(s) 1289
PctI GAATGC 1 cut(s) 1563
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 3 cut(s) 417, 542, 1294
PflMI CCANNNNNTGG 1 cut(s) 1217
PinAI ACCGGT 1 cut(s) 1090
PleI GAGTC 1 cut(s) 780
PmaCI CACGTG 1 cut(s) 1501
PmlI CACGTG 1 cut(s) 1501
PpsI GAGTC 1 cut(s) 780
Ppu21I YACGTR 1 cut(s) 1501
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 127, 1454
PspCI CACGTG 1 cut(s) 1501
PspFI CCCAGC 1 cut(s) 1620
PspGI CCWGG 2 cut(s) 127, 1454
PspN4I GGNNCC 4 cut(s) 459, 569, 1204, 1848
PspPI GGNCC 1 cut(s) 1058
PsrI GAACNNNNNNTAC 2 cut(s) 1362, 1394
PstI CTGCAG 1 cut(s) 1327
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 1783, 1846
RsaI GTAC 7 cut(s) 689, 965, 977, 1238, 1380, 1544, 1827
RsaNI GTAC 7 cut(s) 688, 964, 976, 1237, 1379, 1543, 1826
RseI CAYNNNNRTG 3 cut(s) 156, 1289, 1422
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 2 cut(s) 162, 1727
Sau3AI GATC 7 cut(s) 109, 235, 498, 967, 1081, 1783, 1846
Sau96I GGNCC 1 cut(s) 1058
ScaI AGTACT 1 cut(s) 1827
SchI GAGTC 1 cut(s) 780
ScrFI CCNGG 4 cut(s) 129, 1289, 1398, 1456
SduI GDGCHC 5 cut(s) 460, 609, 669, 768, 1873
SfaNI GCATC 5 cut(s) 962, 1108, 1186, 1645, 1674
SfcI CTRYAG 2 cut(s) 1066, 1323
SinI GGWCC 1 cut(s) 1058
SmiMI CAYNNNNRTG 3 cut(s) 156, 1289, 1422
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SsiI CCGC 7 cut(s) 609, 863, 1007, 1132, 1625, 1679, 1832
SspMI CTAG 6 cut(s) 662, 950, 1079, 1412, 1742, 1859
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 4 cut(s) 127, 1287, 1396, 1454
StyI CCWWGG 2 cut(s) 1126, 1298
TaaI ACNGT 5 cut(s) 445, 1058, 1265, 1385, 1752
TaiI ACGT 3 cut(s) 1446, 1503, 1581
TaqI TCGA 3 cut(s) 17, 545, 1533
TatI WGTACW 4 cut(s) 687, 1236, 1378, 1825
TauI GCSGC 3 cut(s) 866, 1010, 1627
TfiI GAWTC 3 cut(s) 417, 542, 1294
Tru1I TTAA 2 cut(s) 162, 1727
Tru9I TTAA 2 cut(s) 162, 1727
TscAI CASTG 3 cut(s) 57, 752, 1757
TseFI GTSAC 4 cut(s) 1385, 1439, 1486, 1501
TseI GCWGC 9 cut(s) 353, 800, 842, 854, 1121, 1167, 1303, 1322, 1738
Tsp45I GTSAC 4 cut(s) 1385, 1439, 1486, 1501
TspDTI ATGAA 4 cut(s) 146, 162, 929, 1343
TspGWI ACGGA 4 cut(s) 254, 774, 913, 1028
TspRI CASTG 3 cut(s) 57, 752, 1757
Van91I CCANNNNNTGG 1 cut(s) 1217
VneI GTGCAC 1 cut(s) 1869
VpaK11BI GGWCC 1 cut(s) 1058
XapI RAATTY 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 6 cut(s) 662, 950, 1079, 1412, 1742, 1859
ZrmI AGTACT 1 cut(s) 1827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.