Prupe.1G064300_v2.0.a1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000353
Physical Location & Seq
Forward (+)
4579786 .. 4584484
4699 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G064300.1

Sequence Viewer

Length: 2343 bp
ATGAGAAGAGGACAAGCACAAAACAGCGTAGATACGGTGGCAGTGGCAATAGACAAGGACAAATCAAGTCAATATGCTATCAAATGGACAGTTGAAAATATTCTCACCAGGGGCCAAGCTCTCACACTGCTCCATGTTAAACACAGAACTGCAGTTTCCAGCCAAACCAGGGGCATGGTTCCTATATCTGAAGTTACAGAGGAGGCTTCAAAAGCGCACAAAAGCCAACTTGAAGCTCAAGCCAACGAGTTGTTTCTACCGTTCCGCTGCTTTTGCGCAAAAAAGGATTTTTCATCTTTGCAACAGCTAAAATGCAATGAAGTCATAATTGAGGATACAAATACAGCAAAAGCACTGGTTAACTATGTTATCAATAATTCAATTGACATTTTGGTACTTGGCGCACCAGCAAGACACGGCCTTCTCAGATTCAGAACTACGGACGTTCCAAGCACCGTGTCAAAAGAGGCACCGGATTTCTGCACTGTGTATGTCATTGGCAAAGGAAAGGTCTCATCTATGCATTGTGCTACCAGAACATTGCCCCGTAAAGCTTCCTCACATAATCAAATACAGGACCAACCCAGCAAAGTTTTTGAATCAAATGATACATTCAATCGCAACCAGCATCCTAGAGCATCAGCGAGGACTAAGTATCCACCCCGTAGTATACTAGATGGCATTGAAATCAGAGCAACAGCTTCAAATAACAAATCATATGAGCTCTCGTCAGAGACCGATATATCATTCGTGAGTAGTGGAAGGCCAAGTGTGGATAAATTGTTTCCTTCTTTCTATGATGGTCTGGAATCAGGACCTCGGAGTTCAATCAGCTCAGATATGGACACCATAAGCTCTACATCATCTTACTCAGGACACAAGTTCATTGACATGAACTCTTTACAACACGAATTCTCATCCTCCTCAATTGAAAGTGAAAATTCATGGTCATCACGGAATATGGATGAAATGGAAGCTGAAATGAGGAGGCTCAGGCTAGAGCTCAAGCAAACGATGGACATGTACAGTACAGCCTGCAAGGAGGCACTCACGGCAAAACAGAAGGCAATGGAACTTCAACAATGGAAATTGGAAGAAGAACGGAGATTACACGAGGCACAAATAGCTGAGGAAGCTGCATTGGCACTTGCAGATGAGCAGAAAATAAAATGTATGGCAGCCATGGAGGCTGCAGAATCAGCTCAAAGGATTGCATTACTGGAAGCACAGAAAAGGAGGAATGCAGAAATGAAAGCCCTTAGAGAAACTGAGAGGAAGAAGGCATTAGAAGCAAAAACATATGATTTCATGTACAGGAAATACACAATTGAGGAGATCGAAGTAGCAACAAATAATTTTTCAAACGTAAACAAGATTGGGGAAGGAGGCTATGGGCCAGTATACAGAGGTGAACTAGACCACACACCAGTGGCAATAAAAGTTCTATGTCCTGACGCAGCTCAAGGACGGTCACAGTTCCAGCAAGAGGTTCAAGTATTGAGTTGCATGCGCCATCCAAACATGGTTCTCCTTCTTGGAGCCTGCCCAGAGTATGGTTGCCTGGTCTATGAGTACATGGCTAATGGAAGCTTAGAAGACCGTCTCTTCCGGCGAGGTAACACCCCAGTTATTCCTTGGCAACTAAGGTTCCATATAGCTGCAGAAATTGGCACCGGCCTTTTGTTCCTTCATCATACCAAGCCAGAACCCCTTGTACACCGTGACCTGAAACCCGGAAACATTTTGCTAGACCATCACTATGTGAGCAAGATCAGTGATGTTGGTTTGGCCAGGCTAGTCCCTCCATCAGTAGCCGATTCAGTCACTCAATATCATATGACATCAACAGCCGGAACATTCTGTTATATAGACCCAGAGTATCAGCAAACTGGGATGCTTGGAATCAAATCTGATATCTACTCACTTGGGGTCATGTTTCTACAACTAATAACAGCCAAGCCACCAATGGGTTTGACTCATCATGTTCAGCGGGCTATGGAGAACGGGACTTTCCAAGAGATGCTTGACCCTGCTGTTCCTGACTGGCCAGTTGAGGAAGCCTTAAAGTTTGCCAAGATAGCTTTACAATGTGCCGAAATGAGGCGTAAAGACAGACCGGATCTTGGAAATGTTGTGCTACCTGAGCTGAACAGATTGAGAAAACTAGCCGAAGATAACAAGACGTGTGGCATCATCGGTGGTGGTGAAGAATTTTCACCAAGTCATGGCCCCATTTCCACAATACAAGATGTTATAAGTGACGTTGAGCTACCACAGTCTGGATACTATAGCTCAAGCTCAAGGAGGAGTTCAAGGGCATCATCTAATTATGGAAGAAACTAA

Protein Analysis

781

Amino Acids

87.27

Weight (kDa)

6.76

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2255
Acc16I TGCGCA 1 cut(s) 277
AccB1I GGYRCC 2 cut(s) 469, 1670
AccB7I CCANNNNNTGG 3 cut(s) 1553, 2225, 2279
AccI GTMKAC 2 cut(s) 670, 1401
AciI CCGC 2 cut(s) 265, 1990
AclWI GGATC 1 cut(s) 2127
AcoI YGGCCR 2 cut(s) 1788, 2045
AcsI RAATTY 3 cut(s) 911, 940, 2210
AcuI CTGAAG 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 1762
AfaI GTAC 6 cut(s) 396, 1025, 1030, 1313, 1574, 1716
AfiI CCNNNNNNNGG 8 cut(s) 169, 1486, 1553, 1967, 2100, 2123, 2225, 2279
AflIII ACRYGT 2 cut(s) 1020, 2183
AjiI CACGTC 1 cut(s) 2184
AjnI CCWGG 4 cut(s) 107, 167, 1560, 1790
AleI CACNNNNGTG 1 cut(s) 1427
Alw21I GWGCWC 2 cut(s) 726, 1005
Alw26I GTCTC 3 cut(s) 517, 728, 1607
AlwI GGATC 1 cut(s) 2127
AoxI GGCC 8 cut(s) 112, 418, 764, 1394, 1675, 1788, 2045, 2227
ApeKI GCWGC 6 cut(s) 267, 1136, 1178, 1190, 1457, 1658
ApoI RAATTY 3 cut(s) 911, 940, 2210
ArsI GACNNNNNNTTYG 2 cut(s) 495, 527
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 4 cut(s) 217, 278, 404, 1512
AspS9I GGNCC 5 cut(s) 112, 577, 815, 1394, 2228
AsuC2I CCSGG 1 cut(s) 1734
AsuHPI GGTGA 4 cut(s) 97, 1421, 2208, 2216
AvaII GGWCC 2 cut(s) 577, 815
BalI TGGCCA 2 cut(s) 1790, 2047
BanI GGYRCC 2 cut(s) 469, 1670
BanII GRGCYC 2 cut(s) 726, 1005
BauI CACGAG 1 cut(s) 1112
BbsI GAAGAC 1 cut(s) 1602
Bbv12I GWGCWC 2 cut(s) 726, 1005
BbvCI CCTCAGC 1 cut(s) 1128
BbvI GCAGC 6 cut(s) 254, 1123, 1177, 1190, 1469, 1645
BccI CCATC 6 cut(s) 671, 794, 1009, 1521, 1761, 1813
BceAI ACGGC 2 cut(s) 433, 1068
BciT130I CCWGG 4 cut(s) 109, 169, 1562, 1792
BciVI GTATCC 3 cut(s) 328, 666, 2276
BcnI CCSGG 1 cut(s) 1734
BcoDI GTCTC 3 cut(s) 517, 728, 1607
BfaI CTAG 7 cut(s) 633, 674, 998, 1415, 1748, 1796, 2165
BfmI CTRYAG 4 cut(s) 150, 1191, 1659, 2287
BfuI GTATCC 3 cut(s) 328, 666, 2276
BglI GCCNNNNNGGC 1 cut(s) 1187
BisI GCNGC 6 cut(s) 268, 1137, 1179, 1191, 1458, 1659
BlsI GCNGC 6 cut(s) 269, 1138, 1180, 1192, 1459, 1660
Bme1390I CCNGG 5 cut(s) 109, 169, 1562, 1734, 1792
Bme18I GGWCC 2 cut(s) 577, 815
BmgBI CACGTC 1 cut(s) 2184
BmgT120I GGNCC 5 cut(s) 112, 577, 815, 1394, 2228
BmiI GGNNCC 7 cut(s) 113, 180, 471, 1540, 1649, 1672, 2230
BmrFI CCNGG 5 cut(s) 109, 169, 1562, 1734, 1792
BmrI ACTGGG 2 cut(s) 1619, 1899
BmsI GCATC 6 cut(s) 637, 647, 1884, 2010, 2199, 2327
BmuI ACTGGG 2 cut(s) 1619, 1899
BpiI GAAGAC 1 cut(s) 1602
Bpu10I CCTNAGC 3 cut(s) 992, 1128, 2142
BpuEI CTTGAG 5 cut(s) 222, 989, 1446, 2278, 2284
BpuMI CCSGG 1 cut(s) 1734
BsaI GGTCTC 2 cut(s) 517, 728
BsaJI CCNNGG 5 cut(s) 108, 168, 818, 1182, 1634
BsaWI WCCGGW 2 cut(s) 472, 2116
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 1486, 1553, 1967, 2100, 2123, 2225, 2279
Bse118I RCCGGY 1 cut(s) 1673
Bse1I ACTGG 8 cut(s) 360, 1224, 1397, 1427, 1625, 1894, 2048, 2048
Bse3DI GCAATG 3 cut(s) 322, 539, 1074
BseBI CCWGG 4 cut(s) 109, 169, 1562, 1792
BseDI CCNNGG 5 cut(s) 108, 168, 818, 1182, 1634
BseGI GGATG 5 cut(s) 628, 917, 970, 1513, 1899
BseLI CCNNNNNNNGG 8 cut(s) 169, 1486, 1553, 1967, 2100, 2123, 2225, 2279
BseMI GCAATG 3 cut(s) 322, 539, 1074
BseMII CTCAG 7 cut(s) 439, 849, 885, 1006, 1119, 1260, 2133
BseNI ACTGG 8 cut(s) 360, 1224, 1397, 1427, 1625, 1894, 2048, 2048
BseRI GAGGAG 5 cut(s) 215, 913, 1000, 1346, 2320
BseXI GCAGC 6 cut(s) 254, 1123, 1177, 1190, 1469, 1645
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 8 cut(s) 114, 420, 766, 1396, 1677, 1790, 2047, 2229
BshNI GGYRCC 2 cut(s) 469, 1670
BsiHKAI GWGCWC 2 cut(s) 726, 1005
BsiSI CCGG 6 cut(s) 473, 1609, 1674, 1734, 1851, 2117
BslFI GGGAC 2 cut(s) 1784, 2020
BslI CCNNNNNNNGG 8 cut(s) 169, 1486, 1553, 1967, 2100, 2123, 2225, 2279
BsmAI GTCTC 3 cut(s) 517, 728, 1607
BsmBI CGTCTC 1 cut(s) 1607
BsmFI GGGAC 2 cut(s) 1784, 2020
BsmI GAATGC 1 cut(s) 1246
BsnI GGCC 8 cut(s) 114, 420, 766, 1396, 1677, 1790, 2047, 2229
Bso31I GGTCTC 2 cut(s) 517, 728
Bsp1286I GDGCHC 2 cut(s) 726, 1005
Bsp1407I TGTACA 3 cut(s) 1023, 1311, 1714
Bsp143I GATC 3 cut(s) 1335, 1770, 2119
Bsp19I CCATGG 1 cut(s) 1182
BspACI CCGC 2 cut(s) 265, 1990
BspANI GGCC 8 cut(s) 114, 420, 766, 1396, 1677, 1790, 2047, 2229
BspCNI CTCAG 7 cut(s) 438, 848, 884, 1005, 1120, 1261, 2134
BspLI GGNNCC 7 cut(s) 113, 180, 471, 1540, 1649, 1672, 2230
BspMAI CTGCAG 3 cut(s) 154, 1195, 1663
BspPI GGATC 1 cut(s) 2127
BspT107I GGYRCC 2 cut(s) 469, 1670
BspTNI GGTCTC 2 cut(s) 517, 728
BsrDI GCAATG 3 cut(s) 322, 539, 1074
BsrFI RCCGGY 1 cut(s) 1673
BsrGI TGTACA 3 cut(s) 1023, 1311, 1714
BsrI ACTGG 8 cut(s) 360, 1224, 1397, 1427, 1625, 1894, 2048, 2048
BssAI RCCGGY 1 cut(s) 1673
BssECI CCNNGG 5 cut(s) 108, 168, 818, 1182, 1634
BssMI GATC 3 cut(s) 1335, 1770, 2119
BssNAI GTATAC 2 cut(s) 671, 1402
BssSI CACGAG 1 cut(s) 1112
BssT1I CCWWGG 2 cut(s) 1182, 1634
Bst1107I GTATAC 2 cut(s) 671, 1402
Bst2BI CACGAG 1 cut(s) 1112
Bst2UI CCWGG 4 cut(s) 109, 169, 1562, 1792
Bst6I CTCTTC 1 cut(s) 1610
BstAUI TGTACA 3 cut(s) 1023, 1311, 1714
BstC8I GCNNGC 4 cut(s) 1036, 1508, 1543, 1992
BstDSI CCRYGG 1 cut(s) 1182
BstF5I GGATG 5 cut(s) 628, 917, 970, 1513, 1899
BstHHI GCGC 4 cut(s) 217, 278, 404, 1512
BstKTI GATC 3 cut(s) 1338, 1773, 2122
BstMAI GTCTC 3 cut(s) 517, 728, 1607
BstMBI GATC 3 cut(s) 1335, 1770, 2119
BstNI CCWGG 4 cut(s) 109, 169, 1562, 1792
BstNSI RCATGY 2 cut(s) 1024, 1510
BstSCI CCNGG 5 cut(s) 107, 167, 1560, 1732, 1790
BstSFI CTRYAG 4 cut(s) 150, 1191, 1659, 2287
BstV1I GCAGC 6 cut(s) 254, 1123, 1177, 1190, 1469, 1645
BstV2I GAAGAC 1 cut(s) 1602
BstX2I RGATCY 1 cut(s) 2119
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 1 cut(s) 2119
BstZ17I GTATAC 2 cut(s) 671, 1402
BsuI GTATCC 3 cut(s) 328, 666, 2276
BsuRI GGCC 8 cut(s) 114, 420, 766, 1396, 1677, 1790, 2047, 2229
BtgI CCRYGG 1 cut(s) 1182
BtrI CACGTC 1 cut(s) 2184
BtsCI GGATG 5 cut(s) 628, 917, 970, 1513, 1899
BtsI GCAGTG 2 cut(s) 48, 125
BtsIMutI CAGTG 6 cut(s) 48, 125, 353, 483, 1434, 1780
Cac8I GCNNGC 4 cut(s) 1036, 1508, 1543, 1992
CfoI GCGC 4 cut(s) 217, 278, 404, 1512
Cfr10I RCCGGY 1 cut(s) 1673
Cfr13I GGNCC 5 cut(s) 112, 577, 815, 1394, 2228
CseI GACGC 1 cut(s) 1463
Csp6I GTAC 6 cut(s) 395, 1024, 1029, 1312, 1573, 1715
CspCI CAANNNNNGTGG 2 cut(s) 2167, 2202
CviQI GTAC 6 cut(s) 395, 1024, 1029, 1312, 1573, 1715
DpnI GATC 3 cut(s) 1337, 1772, 2121
DpnII GATC 3 cut(s) 1335, 1770, 2119
DraIII CACNNNGTG 1 cut(s) 1762
EaeI YGGCCR 2 cut(s) 1788, 2045
Eam1104I CTCTTC 1 cut(s) 1610
EarI CTCTTC 1 cut(s) 1610
Ecl136II GAGCTC 2 cut(s) 724, 1003
Eco130I CCWWGG 2 cut(s) 1182, 1634
Eco24I GRGCYC 2 cut(s) 726, 1005
Eco31I GGTCTC 2 cut(s) 517, 728
Eco32I GATATC 1 cut(s) 1915
Eco47I GGWCC 2 cut(s) 577, 815
Eco53kI GAGCTC 2 cut(s) 724, 1003
Eco57I CTGAAG 1 cut(s) 210
EcoICRI GAGCTC 2 cut(s) 724, 1003
EcoO109I RGGNCCY 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 911
EcoRII CCWGG 4 cut(s) 107, 167, 1560, 1790
EcoRV GATATC 1 cut(s) 1915
EcoT14I CCWWGG 2 cut(s) 1182, 1634
EcoT22I ATGCAT 1 cut(s) 525
EcoT38I GRGCYC 2 cut(s) 726, 1005
ErhI CCWWGG 2 cut(s) 1182, 1634
Esp3I CGTCTC 1 cut(s) 1607
FalI AAGNNNNNCTT 2 cut(s) 2007, 2039
FaqI GGGAC 2 cut(s) 1784, 2020
FauI CCCGC 1 cut(s) 1983
FauNDI CATATG 3 cut(s) 718, 1300, 1836
FblI GTMKAC 2 cut(s) 670, 1401
Fnu4HI GCNGC 6 cut(s) 268, 1137, 1179, 1191, 1458, 1659
FokI GGATG 5 cut(s) 615, 904, 977, 1500, 1906
FriOI GRGCYC 2 cut(s) 726, 1005
Fsp4HI GCNGC 6 cut(s) 268, 1137, 1179, 1191, 1458, 1659
FspBI CTAG 7 cut(s) 633, 674, 998, 1415, 1748, 1796, 2165
FspI TGCGCA 1 cut(s) 277
GlaI GCGC 4 cut(s) 216, 277, 403, 1511
GluI GCNGC 6 cut(s) 268, 1137, 1179, 1191, 1458, 1659
GsaI CCCAGC 1 cut(s) 588
HaeIII GGCC 8 cut(s) 114, 420, 766, 1396, 1677, 1790, 2047, 2229
HapII CCGG 6 cut(s) 473, 1609, 1674, 1734, 1851, 2117
HgaI GACGC 1 cut(s) 1463
HhaI GCGC 4 cut(s) 217, 278, 404, 1512
Hin6I GCGC 4 cut(s) 215, 276, 402, 1510
HinP1I GCGC 4 cut(s) 215, 276, 402, 1510
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 552, 1588
HinfI GANTC 7 cut(s) 429, 599, 809, 1196, 1817, 1902, 1975
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 6 cut(s) 473, 1609, 1674, 1734, 1851, 2117
HphI GGTGA 4 cut(s) 97, 1421, 2208, 2216
Hpy166II GTNNAC 6 cut(s) 361, 671, 1369, 1402, 1412, 1717
Hpy188I TCNGA 8 cut(s) 190, 428, 434, 692, 733, 822, 838, 1912
Hpy188III TCNNGA 7 cut(s) 751, 806, 813, 873, 1451, 2039, 2280
Hpy8I GTNNAC 6 cut(s) 361, 671, 1369, 1402, 1412, 1717
HpyAV CCTTC 8 cut(s) 431, 756, 798, 1057, 1273, 1376, 1541, 1697
HpyCH4IV ACGT 4 cut(s) 444, 1365, 2183, 2262
HpySE526I ACGT 4 cut(s) 444, 1365, 2183, 2262
HspAI GCGC 4 cut(s) 215, 276, 402, 1510
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 1335, 1770, 2119
LmnI GCTCC 2 cut(s) 135, 1538
Lsp1109I GCAGC 6 cut(s) 254, 1123, 1177, 1190, 1469, 1645
LweI GCATC 6 cut(s) 637, 647, 1884, 2010, 2199, 2327
MaeI CTAG 7 cut(s) 633, 674, 998, 1415, 1748, 1796, 2165
MaeII ACGT 4 cut(s) 444, 1365, 2183, 2262
MaeIII GTNAC 6 cut(s) 193, 1470, 1616, 1721, 1822, 2258
MalI GATC 3 cut(s) 1337, 1772, 2121
MboI GATC 3 cut(s) 1335, 1770, 2119
MboII GAAGA 8 cut(s) 18, 1106, 1109, 1288, 1597, 1607, 2183, 2219
MfeI CAATTG 3 cut(s) 381, 927, 1326
MflI RGATCY 1 cut(s) 2119
MhlI GDGCHC 2 cut(s) 726, 1005
MlsI TGGCCA 2 cut(s) 1790, 2047
MluNI TGGCCA 2 cut(s) 1790, 2047
MlyI GAGTC 1 cut(s) 1969
Mox20I TGGCCA 2 cut(s) 1790, 2047
Mph1103I ATGCAT 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 99
MscI TGGCCA 2 cut(s) 1790, 2047
MseI TTAA 3 cut(s) 138, 360, 2063
MslI CAYNNNNRTG 3 cut(s) 890, 1427, 1758
Msp20I TGGCCA 2 cut(s) 1790, 2047
MspA1I CMGCKG 2 cut(s) 267, 1990
MspI CCGG 6 cut(s) 473, 1609, 1674, 1734, 1851, 2117
MspR9I CCNGG 5 cut(s) 109, 169, 1562, 1734, 1792
MunI CAATTG 3 cut(s) 381, 927, 1326
Mva1269I GAATGC 1 cut(s) 1246
MvaI CCWGG 4 cut(s) 109, 169, 1562, 1792
NciI CCSGG 1 cut(s) 1734
NcoI CCATGG 1 cut(s) 1182
NdeI CATATG 3 cut(s) 718, 1300, 1836
NdeII GATC 3 cut(s) 1335, 1770, 2119
NlaIV GGNNCC 7 cut(s) 113, 180, 471, 1540, 1649, 1672, 2230
NmuCI GTSAC 4 cut(s) 1470, 1721, 1822, 2258
NsbI TGCGCA 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 525
NspI RCATGY 2 cut(s) 1024, 1510
OliI CACNNNNGTG 1 cut(s) 1427
PaeI GCATGC 1 cut(s) 1510
PciI ACATGT 1 cut(s) 1020
PctI GAATGC 1 cut(s) 1246
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 6 cut(s) 429, 599, 809, 1196, 1817, 1902
PflMI CCANNNNNTGG 3 cut(s) 1553, 2225, 2279
PkrI GCNGC 6 cut(s) 269, 1138, 1180, 1192, 1459, 1660
PleI GAGTC 1 cut(s) 1969
PpsI GAGTC 1 cut(s) 1969
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 1020
PsiI TTATAA 1 cut(s) 2255
Psp124BI GAGCTC 2 cut(s) 726, 1005
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 4 cut(s) 107, 167, 1560, 1790
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 4 cut(s) 107, 167, 1560, 1790
PspN4I GGNNCC 7 cut(s) 113, 180, 471, 1540, 1649, 1672, 2230
PspPI GGNCC 5 cut(s) 112, 577, 815, 1394, 2228
PspPPI RGGWCCY 1 cut(s) 815
PsrI GAACNNNNNNTAC 4 cut(s) 1092, 1124, 1698, 1730
PstI CTGCAG 3 cut(s) 154, 1195, 1663
PsuI RGATCY 1 cut(s) 2119
RsaI GTAC 6 cut(s) 396, 1025, 1030, 1313, 1574, 1716
RsaNI GTAC 6 cut(s) 395, 1024, 1029, 1312, 1573, 1715
RseI CAYNNNNRTG 3 cut(s) 890, 1427, 1758
SacI GAGCTC 2 cut(s) 726, 1005
SaqAI TTAA 3 cut(s) 138, 360, 2063
SatI GCNGC 6 cut(s) 268, 1137, 1179, 1191, 1458, 1659
Sau3AI GATC 3 cut(s) 1335, 1770, 2119
Sau96I GGNCC 5 cut(s) 112, 577, 815, 1394, 2228
SchI GAGTC 1 cut(s) 1969
ScrFI CCNGG 5 cut(s) 109, 169, 1562, 1734, 1792
SduI GDGCHC 2 cut(s) 726, 1005
SfaNI GCATC 6 cut(s) 637, 647, 1884, 2010, 2199, 2327
SfcI CTRYAG 4 cut(s) 150, 1191, 1659, 2287
SinI GGWCC 2 cut(s) 577, 815
SmiMI CAYNNNNRTG 3 cut(s) 890, 1427, 1758
SmlI CTYRAG 5 cut(s) 237, 1004, 1461, 2293, 2299
SmoI CTYRAG 5 cut(s) 237, 1004, 1461, 2293, 2299
SphI GCATGC 1 cut(s) 1510
SsiI CCGC 2 cut(s) 265, 1990
SspI AATATT 1 cut(s) 100
SspMI CTAG 7 cut(s) 633, 674, 998, 1415, 1748, 1796, 2165
SstI GAGCTC 2 cut(s) 726, 1005
StyD4I CCNGG 5 cut(s) 107, 167, 1560, 1732, 1790
StyI CCWWGG 2 cut(s) 1182, 1634
TaiI ACGT 4 cut(s) 447, 1368, 2186, 2265
TaqI TCGA 1 cut(s) 1338
TaqII GACCGA 1 cut(s) 752
TatI WGTACW 5 cut(s) 1023, 1028, 1311, 1572, 1714
TfiI GAWTC 6 cut(s) 429, 599, 809, 1196, 1817, 1902
Tru1I TTAA 3 cut(s) 138, 360, 2063
Tru9I TTAA 3 cut(s) 138, 360, 2063
TscAI CASTG 6 cut(s) 48, 132, 360, 490, 1434, 1780
TseFI GTSAC 4 cut(s) 1470, 1721, 1822, 2258
TseI GCWGC 6 cut(s) 267, 1136, 1178, 1190, 1457, 1658
Tsp45I GTSAC 4 cut(s) 1470, 1721, 1822, 2258
TspDTI ATGAA 9 cut(s) 282, 333, 874, 908, 933, 981, 1265, 1297, 1679
TspGWI ACGGA 3 cut(s) 455, 970, 1117
TspRI CASTG 6 cut(s) 48, 132, 360, 490, 1434, 1780
Van91I CCANNNNNTGG 3 cut(s) 1553, 2225, 2279
VpaK11BI GGWCC 2 cut(s) 577, 815
XapI RAATTY 3 cut(s) 911, 940, 2210
XceI RCATGY 2 cut(s) 1024, 1510
XcmI CCANNNNNNNNNTGG 2 cut(s) 1632, 1963
XmiI GTMKAC 2 cut(s) 670, 1401
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 7 cut(s) 633, 674, 998, 1415, 1748, 1796, 2165
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.