U-box domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1022 |
| AccI | GTMKAC | 1 cut(s) | 317 |
| AciI | CCGC | 2 cut(s) | 1203, 1742 |
| AclWI | GGATC | 3 cut(s) | 1334, 1596, 1724 |
| AcoI | YGGCCR | 2 cut(s) | 1257, 1514 |
| AcsI | RAATTY | 2 cut(s) | 368, 1651 |
| AfaI | GTAC | 4 cut(s) | 485, 618, 1043, 1185 |
| AfeI | AGCGCT | 1 cut(s) | 271 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 955, 1022, 1592, 1748 |
| AflIII | ACRYGT | 3 cut(s) | 83, 228, 480 |
| AgsI | TTSAA | 6 cut(s) | 119, 282, 384, 389, 809, 1078 |
| AjiI | CACGTC | 1 cut(s) | 315 |
| AjnI | CCWGG | 1 cut(s) | 1029 |
| AleI | CACNNNNGTG | 1 cut(s) | 896 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 293, 325 |
| Alw21I | GWGCWC | 4 cut(s) | 83, 465, 524, 1564 |
| Alw26I | GTCTC | 1 cut(s) | 1481 |
| AlwI | GGATC | 3 cut(s) | 1334, 1596, 1724 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 185, 1283 |
| Aor51HI | AGCGCT | 1 cut(s) | 271 |
| AoxI | GGCC | 3 cut(s) | 765, 1257, 1514 |
| ApeKI | GCWGC | 7 cut(s) | 4, 644, 656, 665, 926, 1127, 1316 |
| ApoI | RAATTY | 2 cut(s) | 368, 1651 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 388, 420 |
| AspLEI | GCGC | 2 cut(s) | 272, 508 |
| AspS9I | GGNCC | 3 cut(s) | 765, 863, 886 |
| AsuC2I | CCSGG | 1 cut(s) | 1665 |
| AsuHPI | GGTGA | 3 cut(s) | 120, 168, 1422 |
| AsuNHI | GCTAGC | 1 cut(s) | 1546 |
| AvaII | GGWCC | 2 cut(s) | 863, 886 |
| BalI | TGGCCA | 2 cut(s) | 1259, 1516 |
| BanII | GRGCYC | 2 cut(s) | 465, 1179 |
| Bbv12I | GWGCWC | 4 cut(s) | 83, 465, 524, 1564 |
| BbvCI | CCTCAGC | 3 cut(s) | 376, 452, 594 |
| BbvI | GCAGC | 7 cut(s) | 16, 656, 668, 677, 938, 1114, 1328 |
| BccI | CCATC | 1 cut(s) | 242 |
| BciT130I | CCWGG | 1 cut(s) | 1031 |
| BclI | TGATCA | 1 cut(s) | 1722 |
| BcnI | CCSGG | 1 cut(s) | 1665 |
| BcoDI | GTCTC | 1 cut(s) | 1481 |
| BfaI | CTAG | 9 cut(s) | 66, 99, 458, 590, 1181, 1217, 1394, 1547, 1762 |
| BfoI | RGCGCY | 2 cut(s) | 273, 509 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 653 |
| BisI | GCNGC | 7 cut(s) | 5, 645, 657, 666, 927, 1128, 1317 |
| BlpI | GCTNAGC | 1 cut(s) | 355 |
| BlsI | GCNGC | 7 cut(s) | 6, 646, 658, 667, 928, 1129, 1318 |
| Bme1390I | CCNGG | 2 cut(s) | 1031, 1665 |
| Bme18I | GGWCC | 2 cut(s) | 863, 886 |
| BmgBI | CACGTC | 1 cut(s) | 315 |
| BmgT120I | GGNCC | 3 cut(s) | 765, 863, 886 |
| BmiI | GGNNCC | 2 cut(s) | 882, 1009 |
| BmrFI | CCNGG | 2 cut(s) | 1031, 1665 |
| BmrI | ACTGGG | 2 cut(s) | 1088, 1152 |
| BmsI | GCATC | 3 cut(s) | 761, 913, 1080 |
| BmtI | GCTAGC | 1 cut(s) | 1550 |
| BmuI | ACTGGG | 2 cut(s) | 1088, 1152 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 494, 526 |
| BpmI | CTGGAG | 1 cut(s) | 500 |
| Bpu10I | CCTNAGC | 3 cut(s) | 376, 452, 594 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 355 |
| BpuEI | CTTGAG | 2 cut(s) | 449, 915 |
| BpuMI | CCSGG | 1 cut(s) | 1665 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1734 |
| BsaJI | CCNNGG | 1 cut(s) | 1103 |
| BsaWI | WCCGGW | 2 cut(s) | 883, 1585 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 293, 323 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 955, 1022, 1592, 1748 |
| Bse118I | RCCGGY | 1 cut(s) | 57 |
| Bse1I | ACTGG | 7 cut(s) | 866, 896, 1094, 1147, 1363, 1517, 1517 |
| Bse3DI | GCAATG | 1 cut(s) | 159 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1734 |
| BseBI | CCWGG | 1 cut(s) | 1031 |
| BseDI | CCNNGG | 1 cut(s) | 1103 |
| BseGI | GGATG | 2 cut(s) | 427, 982 |
| BseJI | GATNNNNATC | 1 cut(s) | 1734 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 955, 1022, 1592, 1748 |
| BseMI | GCAATG | 1 cut(s) | 159 |
| BseNI | ACTGG | 7 cut(s) | 866, 896, 1094, 1147, 1363, 1517, 1517 |
| BseXI | GCAGC | 7 cut(s) | 16, 656, 668, 677, 938, 1114, 1328 |
| BseYI | CCCAGC | 1 cut(s) | 1366 |
| BsgI | GTGCAG | 1 cut(s) | 1123 |
| BshFI | GGCC | 3 cut(s) | 767, 1259, 1516 |
| BsiHKAI | GWGCWC | 4 cut(s) | 83, 465, 524, 1564 |
| BsiSI | CCGG | 5 cut(s) | 58, 884, 1320, 1586, 1664 |
| BslFI | GGGAC | 2 cut(s) | 1253, 1489 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 955, 1022, 1592, 1748 |
| BsmAI | GTCTC | 1 cut(s) | 1481 |
| BsmBI | CGTCTC | 1 cut(s) | 1481 |
| BsmFI | GGGAC | 2 cut(s) | 1253, 1489 |
| BsmI | GAATGC | 1 cut(s) | 1744 |
| BsnI | GGCC | 3 cut(s) | 767, 1259, 1516 |
| Bsp1286I | GDGCHC | 5 cut(s) | 83, 465, 524, 1179, 1564 |
| Bsp1407I | TGTACA | 2 cut(s) | 483, 616 |
| Bsp143I | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 355 |
| BspACI | CCGC | 2 cut(s) | 1203, 1742 |
| BspANI | GGCC | 3 cut(s) | 767, 1259, 1516 |
| BspLI | GGNNCC | 2 cut(s) | 882, 1009 |
| BspOI | GCTAGC | 1 cut(s) | 1550 |
| BspPI | GGATC | 3 cut(s) | 1334, 1596, 1724 |
| BsrDI | GCAATG | 1 cut(s) | 159 |
| BsrFI | RCCGGY | 1 cut(s) | 57 |
| BsrGI | TGTACA | 2 cut(s) | 483, 616 |
| BsrI | ACTGG | 7 cut(s) | 866, 896, 1094, 1147, 1363, 1517, 1517 |
| BssAI | RCCGGY | 1 cut(s) | 57 |
| BssECI | CCNNGG | 1 cut(s) | 1103 |
| BssMI | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| BssT1I | CCWWGG | 1 cut(s) | 1103 |
| Bst2UI | CCWGG | 1 cut(s) | 1031 |
| Bst4CI | ACNGT | 4 cut(s) | 493, 939, 1190, 1557 |
| Bst6I | CTCTTC | 1 cut(s) | 573 |
| BstAUI | TGTACA | 2 cut(s) | 483, 616 |
| BstC8I | GCNNGC | 5 cut(s) | 63, 104, 212, 977, 1548 |
| BstF5I | GGATG | 2 cut(s) | 427, 982 |
| BstH2I | RGCGCY | 2 cut(s) | 273, 509 |
| BstHHI | GCGC | 2 cut(s) | 272, 508 |
| BstKTI | GATC | 5 cut(s) | 1069, 1342, 1591, 1725, 1732 |
| BstMAI | GTCTC | 1 cut(s) | 1481 |
| BstMBI | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 590, 653, 662, 665, 758, 908, 949, 1547 |
| BstNI | CCWGG | 1 cut(s) | 1031 |
| BstNSI | RCATGY | 3 cut(s) | 232, 484, 979 |
| BstSCI | CCNGG | 2 cut(s) | 1029, 1663 |
| BstV1I | GCAGC | 7 cut(s) | 16, 656, 668, 677, 938, 1114, 1328 |
| BstX2I | RGATCY | 1 cut(s) | 1588 |
| BstYI | RGATCY | 1 cut(s) | 1588 |
| BsuRI | GGCC | 3 cut(s) | 767, 1259, 1516 |
| BtrI | CACGTC | 1 cut(s) | 315 |
| BtsCI | GGATG | 2 cut(s) | 427, 982 |
| BtsIMutI | CAGTG | 5 cut(s) | 903, 1140, 1482, 1562, 1732 |
| Cac8I | GCNNGC | 5 cut(s) | 63, 104, 212, 977, 1548 |
| CaiI | CAGNNNCTG | 2 cut(s) | 185, 1283 |
| CfoI | GCGC | 2 cut(s) | 272, 508 |
| Cfr10I | RCCGGY | 1 cut(s) | 57 |
| Cfr13I | GGNCC | 3 cut(s) | 765, 863, 886 |
| CseI | GACGC | 2 cut(s) | 905, 1498 |
| Csp6I | GTAC | 4 cut(s) | 484, 617, 1042, 1184 |
| CviQI | GTAC | 4 cut(s) | 484, 617, 1042, 1184 |
| DpnI | GATC | 5 cut(s) | 1068, 1341, 1590, 1724, 1731 |
| DpnII | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| EaeI | YGGCCR | 2 cut(s) | 1257, 1514 |
| Eam1104I | CTCTTC | 1 cut(s) | 573 |
| EarI | CTCTTC | 1 cut(s) | 573 |
| Ecl136II | GAGCTC | 1 cut(s) | 463 |
| Eco130I | CCWWGG | 1 cut(s) | 1103 |
| Eco24I | GRGCYC | 2 cut(s) | 465, 1179 |
| Eco32I | GATATC | 2 cut(s) | 775, 834 |
| Eco47I | GGWCC | 2 cut(s) | 863, 886 |
| Eco47III | AGCGCT | 1 cut(s) | 271 |
| Eco53kI | GAGCTC | 1 cut(s) | 463 |
| EcoICRI | GAGCTC | 1 cut(s) | 463 |
| EcoO109I | RGGNCCY | 1 cut(s) | 765 |
| EcoRI | GAATTC | 1 cut(s) | 1651 |
| EcoRII | CCWGG | 1 cut(s) | 1029 |
| EcoRV | GATATC | 2 cut(s) | 775, 834 |
| EcoT14I | CCWWGG | 1 cut(s) | 1103 |
| EcoT38I | GRGCYC | 2 cut(s) | 465, 1179 |
| ErhI | CCWWGG | 1 cut(s) | 1103 |
| Esp3I | CGTCTC | 1 cut(s) | 1481 |
| FaqI | GGGAC | 2 cut(s) | 1253, 1489 |
| FauI | CCCGC | 1 cut(s) | 1210 |
| FbaI | TGATCA | 1 cut(s) | 1722 |
| FblI | GTMKAC | 1 cut(s) | 317 |
| Fnu4HI | GCNGC | 7 cut(s) | 5, 645, 657, 666, 927, 1128, 1317 |
| FokI | GGATG | 2 cut(s) | 434, 969 |
| FriOI | GRGCYC | 2 cut(s) | 465, 1179 |
| Fsp4HI | GCNGC | 7 cut(s) | 5, 645, 657, 666, 927, 1128, 1317 |
| FspBI | CTAG | 9 cut(s) | 66, 99, 458, 590, 1181, 1217, 1394, 1547, 1762 |
| GlaI | GCGC | 2 cut(s) | 271, 507 |
| GluI | GCNGC | 7 cut(s) | 5, 645, 657, 666, 927, 1128, 1317 |
| GsaI | CCCAGC | 1 cut(s) | 1370 |
| GsuI | CTGGAG | 1 cut(s) | 500 |
| HaeII | RGCGCY | 2 cut(s) | 273, 509 |
| HaeIII | GGCC | 3 cut(s) | 767, 1259, 1516 |
| HapII | CCGG | 5 cut(s) | 58, 884, 1320, 1586, 1664 |
| HgaI | GACGC | 2 cut(s) | 905, 1498 |
| HhaI | GCGC | 2 cut(s) | 272, 508 |
| Hin6I | GCGC | 2 cut(s) | 270, 506 |
| HinP1I | GCGC | 2 cut(s) | 270, 506 |
| HincII | GTYRAC | 1 cut(s) | 318 |
| HindII | GTYRAC | 1 cut(s) | 318 |
| HinfI | GANTC | 8 cut(s) | 29, 71, 257, 634, 689, 809, 1122, 1286 |
| HpaII | CCGG | 5 cut(s) | 58, 884, 1320, 1586, 1664 |
| HphI | GGTGA | 3 cut(s) | 120, 168, 1422 |
| Hpy166II | GTNNAC | 4 cut(s) | 318, 871, 1186, 1387 |
| Hpy188I | TCNGA | 6 cut(s) | 28, 76, 292, 757, 1121, 1381 |
| Hpy188III | TCNNGA | 9 cut(s) | 261, 330, 517, 920, 1016, 1343, 1508, 1689, 1749 |
| Hpy8I | GTNNAC | 4 cut(s) | 318, 871, 1186, 1387 |
| Hpy99I | CGWCG | 2 cut(s) | 203, 319 |
| HpyAV | CCTTC | 6 cut(s) | 742, 845, 1010, 1281, 1466, 1591 |
| HpyCH4III | ACNGT | 4 cut(s) | 493, 939, 1190, 1557 |
| HpyCH4IV | ACGT | 3 cut(s) | 85, 198, 314 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 18, 498, 605, 840, 926, 1140 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 590, 653, 662, 665, 758, 908, 949, 1547 |
| HpySE526I | ACGT | 3 cut(s) | 85, 198, 314 |
| HspAI | GCGC | 2 cut(s) | 270, 506 |
| Ksp22I | TGATCA | 1 cut(s) | 1722 |
| Kzo9I | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| LmnI | GCTCC | 3 cut(s) | 314, 1007, 1056 |
| Lsp1109I | GCAGC | 7 cut(s) | 16, 656, 668, 677, 938, 1114, 1328 |
| LweI | GCATC | 3 cut(s) | 761, 913, 1080 |
| MaeI | CTAG | 9 cut(s) | 66, 99, 458, 590, 1181, 1217, 1394, 1547, 1762 |
| MaeII | ACGT | 3 cut(s) | 85, 198, 314 |
| MaeIII | GTNAC | 6 cut(s) | 86, 126, 404, 1085, 1190, 1428 |
| MalI | GATC | 5 cut(s) | 1068, 1341, 1590, 1724, 1731 |
| MboI | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| MboII | GAAGA | 7 cut(s) | 437, 572, 590, 1066, 1076, 1148, 1383 |
| MfeI | CAATTG | 2 cut(s) | 384, 795 |
| MflI | RGATCY | 1 cut(s) | 1588 |
| MhlI | GDGCHC | 5 cut(s) | 83, 465, 524, 1179, 1564 |
| MlsI | TGGCCA | 2 cut(s) | 1259, 1516 |
| MluCI | AATT | 9 cut(s) | 264, 368, 384, 554, 795, 804, 823, 944, 1651 |
| MluNI | TGGCCA | 2 cut(s) | 1259, 1516 |
| Mox20I | TGGCCA | 2 cut(s) | 1259, 1516 |
| MscI | TGGCCA | 2 cut(s) | 1259, 1516 |
| MseI | TTAA | 5 cut(s) | 543, 726, 960, 1467, 1617 |
| MslI | CAYNNNNRTG | 2 cut(s) | 896, 1227 |
| Msp20I | TGGCCA | 2 cut(s) | 1259, 1516 |
| MspI | CCGG | 5 cut(s) | 58, 884, 1320, 1586, 1664 |
| MspR9I | CCNGG | 2 cut(s) | 1031, 1665 |
| MunI | CAATTG | 2 cut(s) | 384, 795 |
| Mva1269I | GAATGC | 1 cut(s) | 1744 |
| MvaI | CCWGG | 1 cut(s) | 1031 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 590, 653, 662, 665, 758, 908, 949, 1547 |
| NciI | CCSGG | 1 cut(s) | 1665 |
| NdeII | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| NheI | GCTAGC | 1 cut(s) | 1546 |
| NlaIV | GGNNCC | 2 cut(s) | 882, 1009 |
| NmuCI | GTSAC | 4 cut(s) | 126, 404, 1190, 1428 |
| NspI | RCATGY | 3 cut(s) | 232, 484, 979 |
| OliI | CACNNNNGTG | 1 cut(s) | 896 |
| PaeI | GCATGC | 1 cut(s) | 979 |
| PciI | ACATGT | 2 cut(s) | 228, 480 |
| PctI | GAATGC | 1 cut(s) | 1744 |
| PfeI | GAWTC | 8 cut(s) | 29, 71, 257, 634, 689, 809, 1122, 1286 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1022 |
| PkrI | GCNGC | 7 cut(s) | 6, 646, 658, 667, 928, 1129, 1318 |
| PscI | ACATGT | 2 cut(s) | 228, 480 |
| Psp124BI | GAGCTC | 1 cut(s) | 465 |
| Psp6I | CCWGG | 1 cut(s) | 1029 |
| PspFI | CCCAGC | 1 cut(s) | 1366 |
| PspGI | CCWGG | 1 cut(s) | 1029 |
| PspN4I | GGNNCC | 2 cut(s) | 882, 1009 |
| PspPI | GGNCC | 3 cut(s) | 765, 863, 886 |
| PstNI | CAGNNNCTG | 2 cut(s) | 185, 1283 |
| PsuI | RGATCY | 1 cut(s) | 1588 |
| RsaI | GTAC | 4 cut(s) | 485, 618, 1043, 1185 |
| RsaNI | GTAC | 4 cut(s) | 484, 617, 1042, 1184 |
| RseI | CAYNNNNRTG | 2 cut(s) | 896, 1227 |
| SacI | GAGCTC | 1 cut(s) | 465 |
| SalI | GTCGAC | 1 cut(s) | 316 |
| SaqAI | TTAA | 5 cut(s) | 543, 726, 960, 1467, 1617 |
| SatI | GCNGC | 7 cut(s) | 5, 645, 657, 666, 927, 1128, 1317 |
| Sau3AI | GATC | 5 cut(s) | 1066, 1339, 1588, 1722, 1729 |
| Sau96I | GGNCC | 3 cut(s) | 765, 863, 886 |
| ScrFI | CCNGG | 2 cut(s) | 1031, 1665 |
| SduI | GDGCHC | 5 cut(s) | 83, 465, 524, 1179, 1564 |
| SfaNI | GCATC | 3 cut(s) | 761, 913, 1080 |
| SinI | GGWCC | 2 cut(s) | 863, 886 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 896, 1227 |
| SmlI | CTYRAG | 2 cut(s) | 464, 930 |
| SmoI | CTYRAG | 2 cut(s) | 464, 930 |
| SphI | GCATGC | 1 cut(s) | 979 |
| Sse9I | AATT | 9 cut(s) | 264, 368, 384, 554, 795, 804, 823, 944, 1651 |
| SsiI | CCGC | 2 cut(s) | 1203, 1742 |
| SspI | AATATT | 1 cut(s) | 278 |
| SspMI | CTAG | 9 cut(s) | 66, 99, 458, 590, 1181, 1217, 1394, 1547, 1762 |
| SstI | GAGCTC | 1 cut(s) | 465 |
| StyD4I | CCNGG | 2 cut(s) | 1029, 1663 |
| StyI | CCWWGG | 1 cut(s) | 1103 |
| TaaI | ACNGT | 4 cut(s) | 493, 939, 1190, 1557 |
| TaiI | ACGT | 3 cut(s) | 88, 201, 317 |
| TaqI | TCGA | 4 cut(s) | 317, 345, 394, 830 |
| TasI | AATT | 9 cut(s) | 264, 368, 384, 554, 795, 804, 823, 944, 1651 |
| TatI | WGTACW | 4 cut(s) | 483, 616, 1041, 1183 |
| TfiI | GAWTC | 8 cut(s) | 29, 71, 257, 634, 689, 809, 1122, 1286 |
| Tru1I | TTAA | 5 cut(s) | 543, 726, 960, 1467, 1617 |
| Tru9I | TTAA | 5 cut(s) | 543, 726, 960, 1467, 1617 |
| TscAI | CASTG | 5 cut(s) | 903, 1147, 1489, 1562, 1732 |
| TseFI | GTSAC | 4 cut(s) | 126, 404, 1190, 1428 |
| TseI | GCWGC | 7 cut(s) | 4, 644, 656, 665, 926, 1127, 1316 |
| Tsp45I | GTSAC | 4 cut(s) | 126, 404, 1190, 1428 |
| TspDTI | ATGAA | 7 cut(s) | 170, 381, 438, 1148, 1263, 1583, 1664 |
| TspRI | CASTG | 5 cut(s) | 903, 1147, 1489, 1562, 1732 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1022 |
| VpaK11BI | GGWCC | 2 cut(s) | 863, 886 |
| XapI | RAATTY | 2 cut(s) | 368, 1651 |
| XceI | RCATGY | 3 cut(s) | 232, 484, 979 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1101, 1432 |
| XmiI | GTMKAC | 1 cut(s) | 317 |
| XspI | CTAG | 9 cut(s) | 66, 99, 458, 590, 1181, 1217, 1394, 1547, 1762 |