U-box domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 211 |
| AccB1I | GGYRCC | 1 cut(s) | 469 |
| AccI | GTMKAC | 1 cut(s) | 1521 |
| AccII | CGCG | 1 cut(s) | 12 |
| AccIII | TCCGGA | 1 cut(s) | 1563 |
| AciI | CCGC | 5 cut(s) | 59, 65, 528, 1220, 2157 |
| AclI | AACGTT | 1 cut(s) | 591 |
| AclWI | GGATC | 3 cut(s) | 519, 1820, 2082 |
| AcoI | YGGCCR | 3 cut(s) | 69, 1386, 2122 |
| AcsI | RAATTY | 1 cut(s) | 1169 |
| AfaI | GTAC | 9 cut(s) | 236, 396, 977, 1268, 1277, 1321, 1508, 1529, 1671 |
| AfeI | AGCGCT | 1 cut(s) | 2118 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1508, 1922, 2078, 2180 |
| AflIII | ACRYGT | 1 cut(s) | 972 |
| AgsI | TTSAA | 7 cut(s) | 257, 603, 723, 1286, 1295, 1435, 2020 |
| AjnI | CCWGG | 1 cut(s) | 2027 |
| Alw21I | GWGCWC | 1 cut(s) | 957 |
| Alw26I | GTCTC | 1 cut(s) | 1562 |
| AlwI | GGATC | 3 cut(s) | 519, 1820, 2082 |
| Ama87I | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| Aor13HI | TCCGGA | 1 cut(s) | 1563 |
| Aor51HI | AGCGCT | 1 cut(s) | 2118 |
| AoxI | GGCC | 9 cut(s) | 69, 136, 663, 1349, 1386, 2000, 2013, 2122, 2182 |
| ApoI | RAATTY | 1 cut(s) | 1169 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 718 |
| AspLEI | GCGC | 1 cut(s) | 2119 |
| AspS9I | GGNCC | 6 cut(s) | 641, 664, 1349, 1480, 1705, 1962 |
| AsuC2I | CCSGG | 1 cut(s) | 1689 |
| AsuHPI | GGTGA | 4 cut(s) | 690, 738, 1376, 2171 |
| AsuNHI | GCTAGC | 2 cut(s) | 584, 2032 |
| AvaI | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| AvaII | GGWCC | 4 cut(s) | 641, 1480, 1705, 1962 |
| BaeGI | GKGCMC | 1 cut(s) | 472 |
| BalI | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| BanI | GGYRCC | 1 cut(s) | 469 |
| BanII | GRGCYC | 2 cut(s) | 851, 957 |
| BbsI | GAAGAC | 1 cut(s) | 1230 |
| Bbv12I | GWGCWC | 1 cut(s) | 957 |
| BccI | CCATC | 7 cut(s) | 80, 415, 565, 911, 1142, 1653, 1768 |
| BciT130I | CCWGG | 1 cut(s) | 2029 |
| BciVI | GTATCC | 1 cut(s) | 328 |
| BclI | TGATCA | 1 cut(s) | 118 |
| BcnI | CCSGG | 1 cut(s) | 1689 |
| BcoDI | GTCTC | 1 cut(s) | 1562 |
| BfaI | CTAG | 4 cut(s) | 585, 950, 1746, 2033 |
| BfmI | CTRYAG | 2 cut(s) | 438, 1101 |
| BfoI | RGCGCY | 1 cut(s) | 2120 |
| BfuI | GTATCC | 1 cut(s) | 328 |
| BglII | AGATCT | 1 cut(s) | 510 |
| Bme1390I | CCNGG | 2 cut(s) | 1689, 2029 |
| Bme18I | GGWCC | 4 cut(s) | 641, 1480, 1705, 1962 |
| BmeT110I | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| BmgT120I | GGNCC | 6 cut(s) | 641, 664, 1349, 1480, 1705, 1962 |
| BmiI | GGNNCC | 5 cut(s) | 471, 643, 829, 1604, 1963 |
| BmrFI | CCNGG | 2 cut(s) | 1689, 2029 |
| BmsI | GCATC | 2 cut(s) | 667, 1399 |
| BmtI | GCTAGC | 2 cut(s) | 588, 2036 |
| BpiI | GAAGAC | 1 cut(s) | 1230 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1598, 2097 |
| BpuEI | CTTGAG | 5 cut(s) | 691, 941, 1310, 1401, 2239 |
| BpuMI | CCSGG | 1 cut(s) | 1689 |
| Bsa29I | ATCGAT | 1 cut(s) | 1824 |
| BsaBI | GATNNNNATC | 1 cut(s) | 433 |
| BsaJI | CCNNGG | 2 cut(s) | 456, 1589 |
| BsaWI | WCCGGW | 3 cut(s) | 1563, 2071, 2195 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1508, 1922, 2078, 2180 |
| Bse118I | RCCGGY | 1 cut(s) | 1844 |
| Bse1I | ACTGG | 1 cut(s) | 2003 |
| Bse3DI | GCAATG | 4 cut(s) | 149, 322, 539, 1098 |
| Bse8I | GATNNNNATC | 1 cut(s) | 433 |
| BseAI | TCCGGA | 1 cut(s) | 1563 |
| BseBI | CCWGG | 1 cut(s) | 2029 |
| BseCI | ATCGAT | 1 cut(s) | 1824 |
| BseDI | CCNNGG | 2 cut(s) | 456, 1589 |
| BseGI | GGATG | 6 cut(s) | 226, 658, 922, 1453, 1468, 2215 |
| BseJI | GATNNNNATC | 1 cut(s) | 433 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1508, 1922, 2078, 2180 |
| BseMI | GCAATG | 4 cut(s) | 149, 322, 539, 1098 |
| BseMII | CTCAG | 4 cut(s) | 142, 207, 1796, 2088 |
| BseNI | ACTGG | 1 cut(s) | 2003 |
| BseRI | GAGGAG | 2 cut(s) | 952, 2024 |
| BseSI | GKGCMC | 1 cut(s) | 472 |
| BseYI | CCCAGC | 3 cut(s) | 623, 672, 1579 |
| BsgI | GTGCAG | 1 cut(s) | 1215 |
| Bsh1236I | CGCG | 1 cut(s) | 12 |
| BshFI | GGCC | 9 cut(s) | 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184 |
| BshNI | GGYRCC | 1 cut(s) | 469 |
| BshVI | ATCGAT | 1 cut(s) | 1824 |
| BsiHKAI | GWGCWC | 1 cut(s) | 957 |
| BsiHKCI | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| BsiSI | CCGG | 6 cut(s) | 662, 1564, 1689, 1845, 2072, 2196 |
| BslFI | GGGAC | 4 cut(s) | 983, 1485, 1739, 1975 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1508, 1922, 2078, 2180 |
| BsmAI | GTCTC | 1 cut(s) | 1562 |
| BsmBI | CGTCTC | 1 cut(s) | 1562 |
| BsmFI | GGGAC | 4 cut(s) | 983, 1485, 1739, 1975 |
| BsmI | GAATGC | 3 cut(s) | 1254, 1423, 2143 |
| BsnI | GGCC | 9 cut(s) | 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184 |
| BsoBI | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| Bsp1286I | GDGCHC | 3 cut(s) | 472, 851, 957 |
| Bsp13I | TCCGGA | 1 cut(s) | 1563 |
| Bsp1407I | TGTACA | 3 cut(s) | 234, 975, 1669 |
| Bsp143I | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| BspACI | CCGC | 5 cut(s) | 59, 65, 528, 1220, 2157 |
| BspANI | GGCC | 9 cut(s) | 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184 |
| BspCNI | CTCAG | 4 cut(s) | 141, 208, 1795, 2089 |
| BspDI | ATCGAT | 1 cut(s) | 1824 |
| BspEI | TCCGGA | 1 cut(s) | 1563 |
| BspFNI | CGCG | 1 cut(s) | 12 |
| BspLI | GGNNCC | 5 cut(s) | 471, 643, 829, 1604, 1963 |
| BspMAI | CTGCAG | 1 cut(s) | 1105 |
| BspOI | GCTAGC | 2 cut(s) | 588, 2036 |
| BspPI | GGATC | 3 cut(s) | 519, 1820, 2082 |
| BspT107I | GGYRCC | 1 cut(s) | 469 |
| BsrDI | GCAATG | 4 cut(s) | 149, 322, 539, 1098 |
| BsrFI | RCCGGY | 1 cut(s) | 1844 |
| BsrGI | TGTACA | 3 cut(s) | 234, 975, 1669 |
| BsrI | ACTGG | 1 cut(s) | 2003 |
| BssAI | RCCGGY | 1 cut(s) | 1844 |
| BssECI | CCNNGG | 2 cut(s) | 456, 1589 |
| BssMI | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| BssT1I | CCWWGG | 1 cut(s) | 1589 |
| Bst2UI | CCWGG | 1 cut(s) | 2029 |
| Bst4CI | ACNGT | 8 cut(s) | 115, 457, 479, 487, 1431, 1511, 1676, 2043 |
| Bst6I | CTCTTC | 6 cut(s) | 41, 48, 1059, 1184, 1281, 1565 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1622 |
| BstAUI | TGTACA | 3 cut(s) | 234, 975, 1669 |
| BstC8I | GCNNGC | 5 cut(s) | 586, 988, 1655, 1850, 2034 |
| BstDEI | CTNAG | 7 cut(s) | 128, 216, 1546, 1598, 1782, 2097, 2142 |
| BstDSI | CCRYGG | 1 cut(s) | 456 |
| BstF5I | GGATG | 6 cut(s) | 226, 658, 922, 1453, 1468, 2215 |
| BstFNI | CGCG | 1 cut(s) | 12 |
| BstH2I | RGCGCY | 1 cut(s) | 2120 |
| BstHHI | GCGC | 1 cut(s) | 2119 |
| BstKTI | GATC | 7 cut(s) | 121, 513, 527, 1555, 1828, 2077, 2230 |
| BstMAI | GTCTC | 1 cut(s) | 1562 |
| BstMBI | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| BstNI | CCWGG | 1 cut(s) | 2029 |
| BstNSI | RCATGY | 4 cut(s) | 976, 1500, 1852, 1978 |
| BstSCI | CCNGG | 2 cut(s) | 1687, 2027 |
| BstSFI | CTRYAG | 2 cut(s) | 438, 1101 |
| BstSLI | GKGCMC | 1 cut(s) | 472 |
| BstUI | CGCG | 1 cut(s) | 12 |
| BstV2I | GAAGAC | 1 cut(s) | 1230 |
| BstX2I | RGATCY | 2 cut(s) | 510, 2074 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1126 |
| BstYI | RGATCY | 2 cut(s) | 510, 2074 |
| Bsu15I | ATCGAT | 1 cut(s) | 1824 |
| BsuI | GTATCC | 1 cut(s) | 328 |
| BsuRI | GGCC | 9 cut(s) | 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184 |
| BsuTUI | ATCGAT | 1 cut(s) | 1824 |
| BtgI | CCRYGG | 1 cut(s) | 456 |
| BtsCI | GGATG | 6 cut(s) | 226, 658, 922, 1453, 1468, 2215 |
| BtsIMutI | CAGTG | 4 cut(s) | 483, 792, 1178, 2048 |
| Cac8I | GCNNGC | 5 cut(s) | 586, 988, 1655, 1850, 2034 |
| CfoI | GCGC | 1 cut(s) | 2119 |
| Cfr10I | RCCGGY | 1 cut(s) | 1844 |
| Cfr13I | GGNCC | 6 cut(s) | 641, 664, 1349, 1480, 1705, 1962 |
| ClaI | ATCGAT | 1 cut(s) | 1824 |
| Csp6I | GTAC | 9 cut(s) | 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 438, 473 |
| CviQI | GTAC | 9 cut(s) | 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670 |
| DdeI | CTNAG | 7 cut(s) | 128, 216, 1546, 1598, 1782, 2097, 2142 |
| DpnI | GATC | 7 cut(s) | 120, 512, 526, 1554, 1827, 2076, 2229 |
| DpnII | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 211 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 211 |
| EaeI | YGGCCR | 3 cut(s) | 69, 1386, 2122 |
| Eam1104I | CTCTTC | 6 cut(s) | 41, 48, 1059, 1184, 1281, 1565 |
| EarI | CTCTTC | 6 cut(s) | 41, 48, 1059, 1184, 1281, 1565 |
| Ecl136II | GAGCTC | 1 cut(s) | 955 |
| Eco130I | CCWWGG | 1 cut(s) | 1589 |
| Eco147I | AGGCCT | 1 cut(s) | 2015 |
| Eco24I | GRGCYC | 2 cut(s) | 851, 957 |
| Eco47I | GGWCC | 4 cut(s) | 641, 1480, 1705, 1962 |
| Eco47III | AGCGCT | 1 cut(s) | 2118 |
| Eco53kI | GAGCTC | 1 cut(s) | 955 |
| Eco88I | CYCGRG | 3 cut(s) | 644, 749, 1502 |
| EcoICRI | GAGCTC | 1 cut(s) | 955 |
| EcoO109I | RGGNCCY | 2 cut(s) | 641, 1962 |
| EcoRI | GAATTC | 1 cut(s) | 1169 |
| EcoRII | CCWGG | 1 cut(s) | 2027 |
| EcoT14I | CCWWGG | 1 cut(s) | 1589 |
| EcoT38I | GRGCYC | 2 cut(s) | 851, 957 |
| ErhI | CCWWGG | 1 cut(s) | 1589 |
| Esp3I | CGTCTC | 1 cut(s) | 1562 |
| FaqI | GGGAC | 4 cut(s) | 983, 1485, 1739, 1975 |
| FauNDI | CATATG | 1 cut(s) | 517 |
| FbaI | TGATCA | 1 cut(s) | 118 |
| FblI | GTMKAC | 1 cut(s) | 1521 |
| FokI | GGATG | 6 cut(s) | 233, 645, 929, 1455, 1460, 2222 |
| FriOI | GRGCYC | 2 cut(s) | 851, 957 |
| FspBI | CTAG | 4 cut(s) | 585, 950, 1746, 2033 |
| GlaI | GCGC | 1 cut(s) | 2118 |
| GsaI | CCCAGC | 3 cut(s) | 627, 676, 1583 |
| HaeII | RGCGCY | 1 cut(s) | 2120 |
| HaeIII | GGCC | 9 cut(s) | 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184 |
| HapII | CCGG | 6 cut(s) | 662, 1564, 1689, 1845, 2072, 2196 |
| HhaI | GCGC | 1 cut(s) | 2119 |
| Hin6I | GCGC | 1 cut(s) | 2117 |
| HinP1I | GCGC | 1 cut(s) | 2117 |
| HindIII | AAGCTT | 1 cut(s) | 717 |
| HinfI | GANTC | 8 cut(s) | 27, 354, 429, 599, 926, 1772, 1884, 2260 |
| HpaII | CCGG | 6 cut(s) | 662, 1564, 1689, 1845, 2072, 2196 |
| HphI | GGTGA | 4 cut(s) | 690, 738, 1376, 2171 |
| Hpy166II | GTNNAC | 6 cut(s) | 8, 235, 1278, 1367, 1522, 1672 |
| Hpy188I | TCNGA | 8 cut(s) | 32, 217, 428, 598, 931, 1607, 1650, 1867 |
| Hpy188III | TCNNGA | 8 cut(s) | 195, 708, 825, 1327, 1406, 1502, 1564, 1829 |
| Hpy8I | GTNNAC | 6 cut(s) | 8, 235, 1278, 1367, 1522, 1672 |
| HpyAV | CCTTC | 7 cut(s) | 777, 1102, 1228, 1238, 1331, 1496, 2077 |
| HpyCH4III | ACNGT | 8 cut(s) | 115, 457, 479, 487, 1431, 1511, 1676, 2043 |
| HpyCH4IV | ACGT | 3 cut(s) | 444, 591, 1402 |
| HpyF3I | CTNAG | 7 cut(s) | 128, 216, 1546, 1598, 1782, 2097, 2142 |
| HpySE526I | ACGT | 3 cut(s) | 444, 591, 1402 |
| HspAI | GCGC | 1 cut(s) | 2117 |
| Kpn2I | TCCGGA | 1 cut(s) | 1563 |
| Ksp22I | TGATCA | 1 cut(s) | 118 |
| Kzo9I | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| LmnI | GCTCC | 2 cut(s) | 559, 1493 |
| LweI | GCATC | 2 cut(s) | 667, 1399 |
| MaeI | CTAG | 4 cut(s) | 585, 950, 1746, 2033 |
| MaeII | ACGT | 3 cut(s) | 444, 591, 1402 |
| MaeIII | GTNAC | 5 cut(s) | 696, 776, 1676, 1777, 2219 |
| MalI | GATC | 7 cut(s) | 120, 512, 526, 1554, 1827, 2076, 2229 |
| MboI | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| MflI | RGATCY | 2 cut(s) | 510, 2074 |
| MhlI | GDGCHC | 3 cut(s) | 472, 851, 957 |
| MlsI | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| MluCI | AATT | 5 cut(s) | 1169, 1608, 1620, 2201, 2237 |
| MluNI | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| MlyI | GAGTC | 3 cut(s) | 1766, 1893, 2269 |
| MmeI | TCCRAC | 1 cut(s) | 473 |
| Mox20I | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| MroI | TCCGGA | 1 cut(s) | 1563 |
| MroXI | GAANNNNTTC | 1 cut(s) | 718 |
| MscI | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| MseI | TTAA | 6 cut(s) | 162, 1212, 1683, 1992, 2103, 2281 |
| MslI | CAYNNNNRTG | 2 cut(s) | 842, 1713 |
| Msp20I | TGGCCA | 3 cut(s) | 71, 1388, 2124 |
| MspA1I | CMGCKG | 1 cut(s) | 1100 |
| MspI | CCGG | 6 cut(s) | 662, 1564, 1689, 1845, 2072, 2196 |
| MspR9I | CCNGG | 2 cut(s) | 1689, 2029 |
| Mva1269I | GAATGC | 3 cut(s) | 1254, 1423, 2143 |
| MvaI | CCWGG | 1 cut(s) | 2029 |
| MvnI | CGCG | 1 cut(s) | 12 |
| NciI | CCSGG | 1 cut(s) | 1689 |
| NdeI | CATATG | 1 cut(s) | 517 |
| NdeII | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| NheI | GCTAGC | 2 cut(s) | 584, 2032 |
| NlaIV | GGNNCC | 5 cut(s) | 471, 643, 829, 1604, 1963 |
| NmuCI | GTSAC | 4 cut(s) | 696, 1676, 1777, 2219 |
| NspI | RCATGY | 4 cut(s) | 976, 1500, 1852, 1978 |
| PaeI | GCATGC | 1 cut(s) | 1852 |
| PceI | AGGCCT | 1 cut(s) | 2015 |
| PciI | ACATGT | 1 cut(s) | 972 |
| PctI | GAATGC | 3 cut(s) | 1254, 1423, 2143 |
| PdmI | GAANNNNTTC | 1 cut(s) | 718 |
| PfeI | GAWTC | 5 cut(s) | 27, 354, 429, 599, 926 |
| PflFI | GACNNNGTC | 1 cut(s) | 1775 |
| PleI | GAGTC | 3 cut(s) | 1766, 1892, 2268 |
| PpsI | GAGTC | 3 cut(s) | 1766, 1892, 2268 |
| PpuMI | RGGWCCY | 2 cut(s) | 641, 1962 |
| PscI | ACATGT | 1 cut(s) | 972 |
| Psp124BI | GAGCTC | 1 cut(s) | 957 |
| Psp1406I | AACGTT | 1 cut(s) | 591 |
| Psp5II | RGGWCCY | 2 cut(s) | 641, 1962 |
| Psp6I | CCWGG | 1 cut(s) | 2027 |
| PspFI | CCCAGC | 3 cut(s) | 623, 672, 1579 |
| PspGI | CCWGG | 1 cut(s) | 2027 |
| PspN4I | GGNNCC | 5 cut(s) | 471, 643, 829, 1604, 1963 |
| PspPI | GGNCC | 6 cut(s) | 641, 664, 1349, 1480, 1705, 1962 |
| PspPPI | RGGWCCY | 2 cut(s) | 641, 1962 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1653, 1685 |
| PstI | CTGCAG | 1 cut(s) | 1105 |
| PsuI | RGATCY | 2 cut(s) | 510, 2074 |
| PsyI | GACNNNGTC | 1 cut(s) | 1775 |
| PvuII | CAGCTG | 1 cut(s) | 1100 |
| RsaI | GTAC | 9 cut(s) | 236, 396, 977, 1268, 1277, 1321, 1508, 1529, 1671 |
| RsaNI | GTAC | 9 cut(s) | 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670 |
| RseI | CAYNNNNRTG | 2 cut(s) | 842, 1713 |
| SacI | GAGCTC | 1 cut(s) | 957 |
| SaqAI | TTAA | 6 cut(s) | 162, 1212, 1683, 1992, 2103, 2281 |
| Sau3AI | GATC | 7 cut(s) | 118, 510, 524, 1552, 1825, 2074, 2227 |
| Sau96I | GGNCC | 6 cut(s) | 641, 664, 1349, 1480, 1705, 1962 |
| SchI | GAGTC | 3 cut(s) | 1766, 1893, 2269 |
| ScrFI | CCNGG | 2 cut(s) | 1689, 2029 |
| SduI | GDGCHC | 3 cut(s) | 472, 851, 957 |
| SfaNI | GCATC | 2 cut(s) | 667, 1399 |
| SfcI | CTRYAG | 2 cut(s) | 438, 1101 |
| SinI | GGWCC | 4 cut(s) | 641, 1480, 1705, 1962 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 842, 1713 |
| SmlI | CTYRAG | 5 cut(s) | 706, 956, 1325, 1416, 2254 |
| SmoI | CTYRAG | 5 cut(s) | 706, 956, 1325, 1416, 2254 |
| SphI | GCATGC | 1 cut(s) | 1852 |
| Sse9I | AATT | 5 cut(s) | 1169, 1608, 1620, 2201, 2237 |
| SseBI | AGGCCT | 1 cut(s) | 2015 |
| SsiI | CCGC | 5 cut(s) | 59, 65, 528, 1220, 2157 |
| SspI | AATATT | 1 cut(s) | 1696 |
| SspMI | CTAG | 4 cut(s) | 585, 950, 1746, 2033 |
| SstI | GAGCTC | 1 cut(s) | 957 |
| StuI | AGGCCT | 1 cut(s) | 2015 |
| StyD4I | CCNGG | 2 cut(s) | 1687, 2027 |
| StyI | CCWWGG | 1 cut(s) | 1589 |
| TaaI | ACNGT | 8 cut(s) | 115, 457, 479, 487, 1431, 1511, 1676, 2043 |
| TaiI | ACGT | 3 cut(s) | 447, 594, 1405 |
| TaqI | TCGA | 5 cut(s) | 75, 560, 1137, 1173, 1824 |
| TasI | AATT | 5 cut(s) | 1169, 1608, 1620, 2201, 2237 |
| TatI | WGTACW | 5 cut(s) | 234, 975, 1275, 1527, 1669 |
| TfiI | GAWTC | 5 cut(s) | 27, 354, 429, 599, 926 |
| Tru1I | TTAA | 6 cut(s) | 162, 1212, 1683, 1992, 2103, 2281 |
| Tru9I | TTAA | 6 cut(s) | 162, 1212, 1683, 1992, 2103, 2281 |
| TscAI | CASTG | 4 cut(s) | 490, 799, 1185, 2048 |
| TseFI | GTSAC | 4 cut(s) | 696, 1676, 1777, 2219 |
| Tsp45I | GTSAC | 4 cut(s) | 696, 1676, 1777, 2219 |
| TspDTI | ATGAA | 7 cut(s) | 146, 333, 421, 826, 1217, 1244, 2150 |
| TspGWI | ACGGA | 1 cut(s) | 272 |
| TspRI | CASTG | 4 cut(s) | 490, 799, 1185, 2048 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1775 |
| VpaK11BI | GGWCC | 4 cut(s) | 641, 1480, 1705, 1962 |
| XapI | RAATTY | 1 cut(s) | 1169 |
| XceI | RCATGY | 4 cut(s) | 976, 1500, 1852, 1978 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1587, 1918 |
| XmiI | GTMKAC | 1 cut(s) | 1521 |
| XmnI | GAANNNNTTC | 1 cut(s) | 718 |
| XspI | CTAG | 4 cut(s) | 585, 950, 1746, 2033 |