Rw4G006520

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000353
Physical Location & Seq
Forward (+)
13584588 .. 13590389
5802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006520.1

Sequence Viewer

Length: 1950 bp
ATGTGGACGGTTGAAAATCTCCTCACCAAAGGCCAATCTCTTACACTAGTTCATGTTAAACAAATAGCATCTGCAAATTCCACACAATCCAGGGGCATTCCCACGTCTGGAGAGAAGGAGGAGTTCAAGATAGTCAACAAAAACCAATTTGATGCTAAGATCAACGAGTTGTTTCTTCCATTCCGCTGGTTTTGCGTAAAAACAGCTATAAAATGCAATGAAGTTGTAATTGAGAACCCTGATGTACCAAAAGCACTGATTAACTATGTTTCCGCTAATTTAATTGACATTTTGGCACTTGGGGCACCATCGACAAGATATGGCCTTCTGCGGTTCAAAAGTACAAATGTTTCAAGCACAGTGTCAAAGGCAGCACCAGAGTTCTGCACTGTGTATGCCGTCGGAAAAGGAAAGTTACCATATGTCCATCTAGATAACATTGAAATCAAGTCACCATTCACAAAAGGAAATGCTTCAATGAATAAATCATATGAACTCTCGACAGAAACTGATATATCGTTTGTCAGTAGTGGAAGGCCAAGCACAGACTGCATGTTTCCTGCTTTCTCTGAGAGTCCAGAATCAGGACCTCGACATTCAATCAGTTCATATATGGACTACAGAAGACCATCTTATTCAGGACGCAAGTTTATTGACATGAGCTCTTCACAATGTGAAGTCTCATCATCCTCAATTAAAAGTGGAAATTCATGGTTATCACAGAATATGGATGGAATGGAAGCTGAGATGAGGAGGCTCAGGCTAGAGCTTAAGCGAACAATGGACATGTACAGTACGGCCTGCAAGGAAGCACTCGCTGAAAAACAAAAGGCAATGCAACTTCACCGATTGAAAATGGAAGAAAAGCAGAGATTAGAAGAGGCACGAGAAGCTGAGGAAGCTGCATTGAAACTTGCAGAGAAGGAGAAAGCAAAATGTCAGGCAGCCATGGATGCTGCAGAAGCAGCTAAAAGGATTGCAGAAGCAGAAGCACAACAGAGGAGGAATGCTGAAATGAAAACCCTTAAAGAAGCTGAAGAGAGGATGAAGGCACTAGGAACAACGACGTACGATCCCAGATACAGGAGATACACAATTAAGGAGATTGAAGTAGCAACAAATAATTTTTCACACGCTCTAGAGGTTGGGGAAGGAGGCTATGGGCCAGTATATAAAGGTGAACTAGACCACACACCAGTGGCAATAAAAGTTCTACGTTCAGATGCAGCTCAAGGATGGTCCCAATTCCAGCAAGAGGTTCATATATTGAGTTGCATACGTCATCCCAATATAATTCTTCGTCTTGGAGCCTGCCCAGAGAATGGATGCCTGGTTTATGAGTACATGGATAATGGGAGCTTAGAAGACCGTCTCTTTCAGCAAGGTAACACCCCAGTTATTCCTTGGCAGCTAAGGTTCCAAATCGCTGCAGATATTGGCACAGGACTGTTGTTCCTTCATCAGACCAAGCCAGAACCACTTGTACACCGTGACCTAAAACCCGGCAACATTTTACTAGACTATATGCTTGTCCCTCCATCAGTAGCTGATTCTATCACTCAATATCATATGACATGTGCTGCCGGAACCTTCTGTTATATTGATCCAGAGTATCAGCAAACTGGCATGCTTGGGATAAAATCTGATATATACTCACTTGGGGTCATGTTCCTACAACTAATAACAGCCAAACCACCTATGGGTTTGACTCATCACGTTGAGCGGGCTATTGAGAAAGGGACTTTCTCTGAGATGCTTGACCCTGCCGTTACTGACTGGCCAGTTGAGGAAGCATTGTGTTTTGCAAAGCTAGCTCTACAGTGTGCTGAGATGAGGCAAAAAGATAGACCGGATCTTGGGAAGGTTGTTCTACCTGAGCTTAACAGATTGAGAAAATTAGCCTGCATAAGATCTATGAGATCAGGAAAACATTATGATTATCTCGAATGA

Protein Analysis

649

Amino Acids

72.98

Weight (kDa)

7.19

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 381 - 617 1.9e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 381 - 622 2.1e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 304
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccBSI CCGCTC 1 cut(s) 1723
AciI CCGC 4 cut(s) 184, 273, 331, 1723
AclWI GGATC 3 cut(s) 1067, 1598, 1860
AcoI YGGCCR 1 cut(s) 1778
AcsI RAATTY 2 cut(s) 76, 706
AcuI CTGAAG 1 cut(s) 1056
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 7 cut(s) 246, 343, 791, 796, 1070, 1343, 1485
AfiI CCNNNNNNNGG 7 cut(s) 107, 584, 1083, 1255, 1322, 1700, 1856
AflII CTTAAG 1 cut(s) 770
AflIII ACRYGT 2 cut(s) 786, 1574
AhlI ACTAGT 1 cut(s) 46
AjiI CACGTC 1 cut(s) 105
AjnI CCWGG 2 cut(s) 89, 1329
AleI CACNNNNGTG 1 cut(s) 1196
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 2 cut(s) 685, 1376
AlwI GGATC 3 cut(s) 1067, 1598, 1860
AlwNI CAGNNNCTG 2 cut(s) 509, 1547
AoxI GGCC 6 cut(s) 31, 322, 536, 798, 1163, 1778
ApeKI GCWGC 9 cut(s) 371, 902, 944, 956, 965, 1226, 1408, 1427, 1580
ApoI RAATTY 2 cut(s) 76, 706
Asp700I GAANNNNTTC 1 cut(s) 472
AspS9I GGNCC 3 cut(s) 587, 1163, 1239
AsuC2I CCSGG 1 cut(s) 1503
AsuHPI GGTGA 4 cut(s) 16, 444, 836, 1190
AsuNHI GCTAGC 1 cut(s) 1810
AvaII GGWCC 2 cut(s) 587, 1239
BaeGI GKGCMC 1 cut(s) 307
BalI TGGCCA 1 cut(s) 1780
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 665
BauI CACGAG 1 cut(s) 885
BbsI GAAGAC 2 cut(s) 631, 1371
Bbv12I GWGCWC 1 cut(s) 665
BbvCI CCTCAGC 1 cut(s) 894
BbvI GCAGC 9 cut(s) 383, 889, 943, 956, 977, 1238, 1414, 1420, 1567
BccI CCATC 6 cut(s) 316, 435, 637, 725, 1230, 1546
BceAI ACGGC 3 cut(s) 383, 813, 1751
BciT130I CCWGG 2 cut(s) 91, 1331
BcnI CCSGG 1 cut(s) 1503
BcoDI GTCTC 2 cut(s) 685, 1376
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 8 cut(s) 47, 431, 764, 1055, 1139, 1184, 1517, 1811
BfmI CTRYAG 4 cut(s) 619, 957, 1428, 1817
BfrI CTTAAG 1 cut(s) 770
BglII AGATCT 1 cut(s) 1910
BisI GCNGC 9 cut(s) 372, 903, 945, 957, 966, 1227, 1409, 1428, 1581
BlsI GCNGC 9 cut(s) 373, 904, 946, 958, 967, 1228, 1410, 1429, 1582
Bme1390I CCNGG 3 cut(s) 91, 1331, 1503
Bme18I GGWCC 2 cut(s) 587, 1239
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 3 cut(s) 587, 1163, 1239
BmiI GGNNCC 5 cut(s) 306, 1241, 1309, 1418, 1588
BmrFI CCNGG 3 cut(s) 91, 1331, 1503
BmrI ACTGGG 1 cut(s) 1388
BmsI GCATC 6 cut(s) 77, 142, 943, 1213, 1316, 1743
BmtI GCTAGC 1 cut(s) 1814
BmuI ACTGGG 1 cut(s) 1388
BpiI GAAGAC 2 cut(s) 631, 1371
BpmI CTGGAG 1 cut(s) 129
Bpu10I CCTNAGC 4 cut(s) 758, 894, 1412, 1875
BpuEI CTTGAG 1 cut(s) 1215
BpuMI CCSGG 1 cut(s) 1503
BsaJI CCNNGG 3 cut(s) 90, 948, 1403
BsaWI WCCGGW 1 cut(s) 1849
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 584, 1083, 1255, 1322, 1700, 1856
Bse1I ACTGG 6 cut(s) 1166, 1196, 1394, 1627, 1781, 1781
Bse3DI GCAATG 2 cut(s) 223, 840
BseBI CCWGG 2 cut(s) 91, 1331
BseDI CCNNGG 3 cut(s) 90, 948, 1403
BseGI GGATG 7 cut(s) 686, 736, 958, 1050, 1241, 1282, 1331
BseLI CCNNNNNNNGG 7 cut(s) 107, 584, 1083, 1255, 1322, 1700, 1856
BseMI GCAATG 2 cut(s) 223, 840
BseMII CTCAG 7 cut(s) 561, 735, 772, 885, 1740, 1818, 1866
BseNI ACTGG 6 cut(s) 1166, 1196, 1394, 1627, 1781, 1781
BseRI GAGGAG 4 cut(s) 11, 134, 766, 1015
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 9 cut(s) 383, 889, 943, 956, 977, 1238, 1414, 1420, 1567
BsgI GTGCAG 1 cut(s) 370
BshFI GGCC 6 cut(s) 33, 324, 538, 800, 1165, 1780
BshNI GGYRCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 665
BsiSI CCGG 3 cut(s) 1503, 1584, 1850
BsiWI CGTACG 1 cut(s) 1068
BslFI GGGAC 3 cut(s) 1225, 1517, 1753
BslI CCNNNNNNNGG 7 cut(s) 107, 584, 1083, 1255, 1322, 1700, 1856
BsmAI GTCTC 2 cut(s) 685, 1376
BsmBI CGTCTC 1 cut(s) 1376
BsmFI GGGAC 3 cut(s) 1225, 1517, 1753
BsmI GAATGC 2 cut(s) 96, 1012
BsnI GGCC 6 cut(s) 33, 324, 538, 800, 1165, 1780
Bsp1286I GDGCHC 2 cut(s) 307, 665
Bsp1407I TGTACA 2 cut(s) 789, 1483
Bsp143I GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
Bsp19I CCATGG 1 cut(s) 948
BspACI CCGC 4 cut(s) 184, 273, 331, 1723
BspANI GGCC 6 cut(s) 33, 324, 538, 800, 1165, 1780
BspCNI CTCAG 7 cut(s) 562, 736, 771, 886, 1741, 1819, 1867
BspLI GGNNCC 5 cut(s) 306, 1241, 1309, 1418, 1588
BspMAI CTGCAG 2 cut(s) 961, 1432
BspOI GCTAGC 1 cut(s) 1814
BspPI GGATC 3 cut(s) 1067, 1598, 1860
BspQI GCTCTTC 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 304
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 1723
BsrDI GCAATG 2 cut(s) 223, 840
BsrGI TGTACA 2 cut(s) 789, 1483
BsrI ACTGG 6 cut(s) 1166, 1196, 1394, 1627, 1781, 1781
BssECI CCNNGG 3 cut(s) 90, 948, 1403
BssMI GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
BssSI CACGAG 1 cut(s) 885
BssT1I CCWWGG 2 cut(s) 948, 1403
Bst2BI CACGAG 1 cut(s) 885
Bst2UI CCWGG 2 cut(s) 91, 1331
Bst4CI ACNGT 8 cut(s) 10, 361, 391, 794, 1370, 1449, 1490, 1821
Bst6I CTCTTC 3 cut(s) 670, 873, 1032
BstAFI CTTAAG 1 cut(s) 770
BstAPI GCANNNNNTGC 1 cut(s) 549
BstAUI TGTACA 2 cut(s) 789, 1483
BstC8I GCNNGC 6 cut(s) 802, 1312, 1628, 1725, 1812, 1903
BstDSI CCRYGG 1 cut(s) 948
BstF5I GGATG 7 cut(s) 686, 736, 958, 1050, 1241, 1282, 1331
BstKTI GATC 6 cut(s) 162, 1075, 1606, 1855, 1913, 1922
BstMAI GTCTC 2 cut(s) 685, 1376
BstMBI GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
BstMWI GCNNNNNNNGC 9 cut(s) 192, 302, 549, 890, 899, 953, 962, 965, 1811
BstNI CCWGG 2 cut(s) 91, 1331
BstNSI RCATGY 4 cut(s) 556, 790, 1578, 1630
BstSCI CCNGG 3 cut(s) 89, 1329, 1501
BstSFI CTRYAG 4 cut(s) 619, 957, 1428, 1817
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 9 cut(s) 383, 889, 943, 956, 977, 1238, 1414, 1420, 1567
BstV2I GAAGAC 2 cut(s) 631, 1371
BstX2I RGATCY 2 cut(s) 1852, 1910
BstXI CCANNNNNNTGG 1 cut(s) 186
BstYI RGATCY 2 cut(s) 1852, 1910
BsuRI GGCC 6 cut(s) 33, 324, 538, 800, 1165, 1780
BtgI CCRYGG 1 cut(s) 948
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 7 cut(s) 686, 736, 958, 1050, 1241, 1282, 1331
BtsIMutI CAGTG 5 cut(s) 254, 366, 387, 1203, 1826
Cac8I GCNNGC 6 cut(s) 802, 1312, 1628, 1725, 1812, 1903
CaiI CAGNNNCTG 2 cut(s) 509, 1547
Cfr13I GGNCC 3 cut(s) 587, 1163, 1239
CseI GACGC 1 cut(s) 651
Csp6I GTAC 7 cut(s) 245, 342, 790, 795, 1069, 1342, 1484
CviQI GTAC 7 cut(s) 245, 342, 790, 795, 1069, 1342, 1484
DpnI GATC 6 cut(s) 161, 1074, 1605, 1854, 1912, 1921
DpnII GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 1778
Eam1104I CTCTTC 3 cut(s) 670, 873, 1032
EarI CTCTTC 3 cut(s) 670, 873, 1032
Ecl136II GAGCTC 1 cut(s) 663
Eco130I CCWWGG 2 cut(s) 948, 1403
Eco24I GRGCYC 1 cut(s) 665
Eco47I GGWCC 2 cut(s) 587, 1239
Eco53kI GAGCTC 1 cut(s) 663
Eco57I CTGAAG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 2 cut(s) 89, 1329
EcoT14I CCWWGG 2 cut(s) 948, 1403
EcoT38I GRGCYC 1 cut(s) 665
ErhI CCWWGG 2 cut(s) 948, 1403
Esp3I CGTCTC 1 cut(s) 1376
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FaqI GGGAC 3 cut(s) 1225, 1517, 1753
FauI CCCGC 1 cut(s) 1716
FauNDI CATATG 3 cut(s) 421, 490, 1569
Fnu4HI GCNGC 9 cut(s) 372, 903, 945, 957, 966, 1227, 1409, 1428, 1581
FokI GGATG 7 cut(s) 673, 743, 965, 1057, 1248, 1269, 1338
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 9 cut(s) 372, 903, 945, 957, 966, 1227, 1409, 1428, 1581
FspBI CTAG 8 cut(s) 47, 431, 764, 1055, 1139, 1184, 1517, 1811
GluI GCNGC 9 cut(s) 372, 903, 945, 957, 966, 1227, 1409, 1428, 1581
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 6 cut(s) 33, 324, 538, 800, 1165, 1780
HapII CCGG 3 cut(s) 1503, 1584, 1850
HgaI GACGC 1 cut(s) 651
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 574, 581, 1550, 1708
HpaII CCGG 3 cut(s) 1503, 1584, 1850
HphI GGTGA 4 cut(s) 16, 444, 836, 1190
Hpy166II GTNNAC 4 cut(s) 6, 136, 1181, 1486
Hpy188I TCNGA 6 cut(s) 404, 571, 1222, 1464, 1645, 1750
Hpy8I GTNNAC 4 cut(s) 6, 136, 1181, 1486
Hpy99I CGWCG 2 cut(s) 404, 1069
HpyAV CCTTC 9 cut(s) 109, 335, 528, 916, 1042, 1145, 1466, 1600, 1855
HpyCH4III ACNGT 8 cut(s) 10, 361, 391, 794, 1370, 1449, 1490, 1821
HpyCH4IV ACGT 5 cut(s) 104, 1067, 1216, 1279, 1716
HpyF10VI GCNNNNNNNGC 9 cut(s) 192, 302, 549, 890, 899, 953, 962, 965, 1811
HpySE526I ACGT 5 cut(s) 104, 1067, 1216, 1279, 1716
Kzo9I GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
LguI GCTCTTC 1 cut(s) 670
LmnI GCTCC 2 cut(s) 1307, 1356
Lsp1109I GCAGC 9 cut(s) 383, 889, 943, 956, 977, 1238, 1414, 1420, 1567
LweI GCATC 6 cut(s) 77, 142, 943, 1213, 1316, 1743
MaeI CTAG 8 cut(s) 47, 431, 764, 1055, 1139, 1184, 1517, 1811
MaeII ACGT 5 cut(s) 104, 1067, 1216, 1279, 1716
MaeIII GTNAC 5 cut(s) 414, 450, 1385, 1490, 1768
MalI GATC 6 cut(s) 161, 1074, 1605, 1854, 1912, 1921
MbiI CCGCTC 1 cut(s) 1723
MboI GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
MboII GAAGA 8 cut(s) 167, 636, 657, 872, 890, 1049, 1289, 1376
MflI RGATCY 2 cut(s) 1852, 1910
MhlI GDGCHC 2 cut(s) 307, 665
MlsI TGGCCA 1 cut(s) 1780
MluNI TGGCCA 1 cut(s) 1780
MlyI GAGTC 2 cut(s) 583, 1702
MmeI TCCRAC 1 cut(s) 382
Mox20I TGGCCA 1 cut(s) 1780
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 1780
MseI TTAA 8 cut(s) 57, 261, 281, 696, 771, 1026, 1098, 1881
MslI CAYNNNNRTG 1 cut(s) 1196
Msp20I TGGCCA 1 cut(s) 1780
MspA1I CMGCKG 1 cut(s) 186
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 3 cut(s) 1503, 1584, 1850
MspR9I CCNGG 3 cut(s) 91, 1331, 1503
Mva1269I GAATGC 2 cut(s) 96, 1012
MvaI CCWGG 2 cut(s) 91, 1331
MwoI GCNNNNNNNGC 9 cut(s) 192, 302, 549, 890, 899, 953, 962, 965, 1811
NciI CCSGG 1 cut(s) 1503
NcoI CCATGG 1 cut(s) 948
NdeI CATATG 3 cut(s) 421, 490, 1569
NdeII GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
NheI GCTAGC 1 cut(s) 1810
NlaIV GGNNCC 5 cut(s) 306, 1241, 1309, 1418, 1588
NmuCI GTSAC 2 cut(s) 450, 1490
NspI RCATGY 4 cut(s) 556, 790, 1578, 1630
OliI CACNNNNGTG 1 cut(s) 1196
PaeI GCATGC 1 cut(s) 1630
PciI ACATGT 2 cut(s) 786, 1574
PciSI GCTCTTC 1 cut(s) 670
PctI GAATGC 2 cut(s) 96, 1012
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 581, 1550
Pfl23II CGTACG 1 cut(s) 1068
PflMI CCANNNNNTGG 1 cut(s) 1322
PkrI GCNGC 9 cut(s) 373, 904, 946, 958, 967, 1228, 1410, 1429, 1582
PleI GAGTC 2 cut(s) 582, 1702
PpsI GAGTC 2 cut(s) 582, 1702
PpuMI RGGWCCY 1 cut(s) 587
PscI ACATGT 2 cut(s) 786, 1574
Psp124BI GAGCTC 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 89, 1329
PspGI CCWGG 2 cut(s) 89, 1329
PspLI CGTACG 1 cut(s) 1068
PspN4I GGNNCC 5 cut(s) 306, 1241, 1309, 1418, 1588
PspPI GGNCC 3 cut(s) 587, 1163, 1239
PspPPI RGGWCCY 1 cut(s) 587
PsrI GAACNNNNNNTAC 2 cut(s) 1467, 1499
PstI CTGCAG 2 cut(s) 961, 1432
PstNI CAGNNNCTG 2 cut(s) 509, 1547
PsuI RGATCY 2 cut(s) 1852, 1910
RsaI GTAC 7 cut(s) 246, 343, 791, 796, 1070, 1343, 1485
RsaNI GTAC 7 cut(s) 245, 342, 790, 795, 1069, 1342, 1484
RseI CAYNNNNRTG 1 cut(s) 1196
SacI GAGCTC 1 cut(s) 665
SapI GCTCTTC 1 cut(s) 670
SaqAI TTAA 8 cut(s) 57, 261, 281, 696, 771, 1026, 1098, 1881
SatI GCNGC 9 cut(s) 372, 903, 945, 957, 966, 1227, 1409, 1428, 1581
Sau3AI GATC 6 cut(s) 159, 1072, 1603, 1852, 1910, 1919
Sau96I GGNCC 3 cut(s) 587, 1163, 1239
SchI GAGTC 2 cut(s) 583, 1702
ScrFI CCNGG 3 cut(s) 91, 1331, 1503
SduI GDGCHC 2 cut(s) 307, 665
SfaNI GCATC 6 cut(s) 77, 142, 943, 1213, 1316, 1743
SfcI CTRYAG 4 cut(s) 619, 957, 1428, 1817
SinI GGWCC 2 cut(s) 587, 1239
SmiMI CAYNNNNRTG 1 cut(s) 1196
SmlI CTYRAG 2 cut(s) 770, 1230
SmoI CTYRAG 2 cut(s) 770, 1230
SpeI ACTAGT 1 cut(s) 46
SphI GCATGC 1 cut(s) 1630
SsiI CCGC 4 cut(s) 184, 273, 331, 1723
SspMI CTAG 8 cut(s) 47, 431, 764, 1055, 1139, 1184, 1517, 1811
SstI GAGCTC 1 cut(s) 665
StyD4I CCNGG 3 cut(s) 89, 1329, 1501
StyI CCWWGG 2 cut(s) 948, 1403
TaaI ACNGT 8 cut(s) 10, 361, 391, 794, 1370, 1449, 1490, 1821
TaiI ACGT 5 cut(s) 107, 1070, 1219, 1282, 1719
TaqI TCGA 4 cut(s) 311, 500, 592, 1944
TatI WGTACW 4 cut(s) 341, 789, 1341, 1483
TfiI GAWTC 2 cut(s) 581, 1550
Tru1I TTAA 8 cut(s) 57, 261, 281, 696, 771, 1026, 1098, 1881
Tru9I TTAA 8 cut(s) 57, 261, 281, 696, 771, 1026, 1098, 1881
TscAI CASTG 5 cut(s) 261, 366, 394, 1203, 1826
TseFI GTSAC 2 cut(s) 450, 1490
TseI GCWGC 9 cut(s) 371, 902, 944, 956, 965, 1226, 1408, 1427, 1580
Tsp45I GTSAC 2 cut(s) 450, 1490
TspRI CASTG 5 cut(s) 261, 366, 394, 1203, 1826
Van91I CCANNNNNTGG 1 cut(s) 1322
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 587, 1239
XapI RAATTY 2 cut(s) 76, 706
XbaI TCTAGA 2 cut(s) 430, 1138
XceI RCATGY 4 cut(s) 556, 790, 1578, 1630
XcmI CCANNNNNNNNNTGG 2 cut(s) 1401, 1696
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 8 cut(s) 47, 431, 764, 1055, 1139, 1184, 1517, 1811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.