Rh2BG365600

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000353
Physical Location & Seq
Forward (+)
50820934 .. 50835831
14898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG365600.1

Sequence Viewer

Length: 3426 bp
ATGAGAGGTAGAGGGCACGACGCAGAACAACAATTAAAAGAACAGGCAGTGGCAGTGGCCATAGACAAGGACAAAGGAAGTGAATATGCAATCAAATGGGCTATTGATAGTCTCTTAACCAAGGGCCAACCTCTCACATTGCTCCATGTTAGACATCCATATCCTGGCCGGACTGCGTCAACGGAGAATCAAAAAGATCCCCAATCACATGAGTTATTTCTTTCGTTTCGTTCCTTTTGCGCGAAAAAAAATATAAAATGCACTGAAGTCATACTCGAGGACAATGACATACCAAACGCACTGATCAAGTATGTTAATGCAAATTCAATTGAGATTTTGGTACTTGGTGTTCCAACAAGAGGTGGCATTTTCAGATTCAAATTTACAGATGTACCGAGCAGTGTGATAAAAATGACGCCAGAATTTTGCTCTGTTTATGTCATCGCTAAAGGAAAAATCTCATCTATGCGATGCTCCACCGTAGCACCGCCACAGCCTCCTCCACGTAGTAATCATCAAATGCAACACCATCCCAGCAATGTTTCTGAAGCAAGTGATTCGCATTTCATCTGCACCCAGAAGCCTAACAGCAGAGCATCAGAGAGGTTGCATTATCAATCGGGTAGCCAGCAGTTTGATGAAATCAAGTCACCATTCACTAGAGGAAGACCTTCGATGGACAGATCATTTGAGCTCCCACCAGATAGTGACATATCATTTGTGAGTACTGGAAGACCAAGTGTGGATCGCATGTATCCTTCTTTCTATGATAGTATGGATTCAGGGAGGAACTCTCGACGTTTGATTGGCTCAGAAACGGAAAACAGAAGCTCTGTGTCATCCAACCCGAGAAGCAAGCATTTTATGGAAATCAACTCTCCACAACTTGAATTTTCACCATCCTCAGTTGATAGTGGAAACTCTTGGTTGTCACAAAATAATAATAATAATTTGGAAGAGGTGGAGCTTGAGATGAGGAGGCTCAGGCTAGAGCTCAAGCAAACAATGGAGATGTACAATACAGCGTGCAAAGAAGCACTGACAGCAAAACATAAGGAAAATGAATTTAACCGGTGTAAAATGGAAGAAGGACAAAGATTAGAACAGGCGCGACTAGCTGAGGAAGCTGAATTGGCACTTGTAGAGAGGGAGAAAGCAAAATGCAAGGCAGCGATTGAAGCAGCTGCAGCAGCTCAAAGGCTTGCTGAACTGGAAGCGCAAAAGAGGAGGAACGCAGAAATGAAAGCCCTTAAAGAATCTGAAGAGAGGAAGAAGACACTTGAAGCAAAGTCATATGATGTTAGGTACAGGAAGTACACAATTGAGGAGATTGAAGTGGCAACAGATAAATTTTCACTAGCTCGCAAGGTTGGGGAAGGAGGCTATGGGCCAGTATACAGAGGTGAACTGCATCATACACCAGTTGCTATAAAAGTTCTACGTCCGGATGCAGCTCAAGGGCGATCGCAGTTCCAGCTAGAGGTTGAAGTATTGAGCTGCATACGACATCCAAACATGGTTCTCCTTCTTGGAGCTTGTCCAGAGTACGGTTGTTTAGTCTATGAGTACATGGCTAATGGGAGCTTGGAAAATCGTCTATTTCACCGAGGTAACACCCCGGCTATTCCTTGGCAACTAAGGTTTCGAATTGCTGCAGAAATTGCAACGGGGCTGTTATTCCTTCATCAGGCTAAGCCAGAACCCCTTGTGCACCGTGACTTAAAACCTGGCAACATATTGCTAGATAATAACTATGTGAGCAAGATTAGTGATGTAGGTTTGGCTAGACTTGTGCCTCCATCAATAGCTGATGCGGTCACACAGTATCATATGACATCAACAGCTGGAACATTTTGCTACATTGATCCAGAGTATCAGCAAACAGGCATGCTTGGAACAAAATCTGATATCTACTCACTAGGTGTGATGCTTCTACAAATCATTACAGCCAAACCACCAATGGGTTTGACTCATCATGTAGAGCGGGCTATTGAGACGGGAAGCTTTAGTGAGATGCTTGATCCTGCAGTTCCTGATTGGCCAGTTGAGGAAGCCTTGAAGTTTGCCAAACTTTCTTTACAGTGTGCTGAAATGAGAAAAAAAGACCGACCCGATCTTGGGAAGGTTATACTGCCTGAGCTTAACAGATTGAGAGCCCTCGCCGACGATAGCTTCCAATGTGTTATGTTTGGCGGAAGTGGAGTACTCTCACCAAATCAAGACTCAAATTCTACATCACAAGATGTCATAAGTCGACCACAATCTGGATATGATAGCTTGAGGAGTCATCTCTCACTCTCGCGCTTCCTACGATCAACCCAATCACTGGAGATGGGAATATATCTGAGCGCTCCGAAAACCGATAAGGTGTCAGAAGATGGTGAGAATGAGAGAGTGCGATTTGGGGCTTCCTCCATGCAGGGGTGGCGTTCAACCATGGAAGATGCTCATGCTGCTTTTCCAGATCTCGACAATTCAACATCTTTCTTTGGTGTTTATGATGGTCATGGAGGTAAAGCGGTGGCAAATTTCTGTGCCAAGTATCTCCACTTACAAGTGCTCAACCAGGAAGCATATCTAGCTGGTGATTTAGGCACTTCCCTGCAGAAAGCTTTTCTCAGGATGGATGAGATGATGCGTGGACAGAGAGGATGGAGAGAACTAGCTGTATTGGGAGATAAGATAGACAAAGTTTCAGGCCTTATTGAAGGGTTCATCTTTTCTCCTAAGAGTGTTGAAGCTAAGAGAAGTGCATTCAATGATCAAATTGATCAGTGGTCTTCTGAGGAGGGGCCTCACTCTGATTTTGATGGACCAAACTCTGGAAGCACAGCTTGTGTTGCTATCATCCGAAACAATCAACTTGTTGTTGCCAATGCTGGTGATTCGCGTTGTGTAATATCGAGGAAGGGTCAGGCATATCCTTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAGGATAGAATTCTGAAGGCCGGTGGATTTATCCAAGTTGGACGGGTCAATGGAAGTTTGAACTTGGCAAGAGCGATTGGAGATGTGGAATTCAAGCAAAACAAACAATTGCCTGTTGAAAAGCAGATTGTAACTGCCAATCCAGATGTAACAAGTGTTGAGATTTGTGATGATGATGAGTTTCTTGTCATCGCTTGTGATGGGATATGGGACTGCATGTCAAACCAACAACTTGTGGATTATGTTGGGGAACAATTGAAACATGAACGTAAACTCTCGGTGGTTTGTGAGAGAGTGTTTGAAAGGTGCTTGGCACCCTCGACAGGTGGTGAAGGCTGTGACAACATGACAATGATCTTGGTTCAGTTTAAGAAGCCTATTATTTCAACTGCTTCTACTGGGAACAAGCCTCTTTCGTCTAATCCATCACTTGAGAAGGACAAGAGCTCGGGAAAAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

1141

Amino Acids

127.1

Weight (kDa)

6.43

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 17 - 140 6.3e-11 Universal stress protein family
Pkinase PF00069 452 - 704 2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 453 - 709 1.3e-37 Protein tyrosine and serine/threonine kinase
PP2C PF00481 800 - 881 2e-11 Protein phosphatase 2C
PP2C PF00481 913 - 1095 1.9e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 3274
AccB7I CCANNNNNTGG 4 cut(s) 164, 2264, 2326, 2822
AccBSI CCGCTC 1 cut(s) 1984
AccI GTMKAC 2 cut(s) 1395, 2254
AccII CGCG 4 cut(s) 242, 1111, 2302, 2890
AccIII TCCGGA 1 cut(s) 1444
AciI CCGC 5 cut(s) 488, 1814, 1984, 2193, 2519
AclWI GGATC 4 cut(s) 191, 753, 1859, 2015
AcoI YGGCCR 3 cut(s) 57, 166, 2039
AcuI CTGAAG 4 cut(s) 285, 567, 1281, 2996
AcyI GRCGYC 1 cut(s) 416
AdeI CACNNNGTG 1 cut(s) 1922
AfaI GTAC 9 cut(s) 342, 393, 727, 1016, 1307, 1316, 1547, 1568, 2205
AfeI AGCGCT 1 cut(s) 2350
AgeI ACCGGT 1 cut(s) 1071
AhdI GACNNNNNGTC 1 cut(s) 3178
AjnI CCWGG 3 cut(s) 163, 1726, 2565
Alw21I GWGCWC 5 cut(s) 696, 996, 1713, 2562, 3410
Alw26I GTCTC 2 cut(s) 116, 1988
Alw44I GTGCAC 1 cut(s) 1709
AlwI GGATC 4 cut(s) 191, 753, 1859, 2015
AlwNI CAGNNNCTG 1 cut(s) 2033
Ama87I CYCGRG 3 cut(s) 275, 847, 3409
Aor13HI TCCGGA 1 cut(s) 1444
Aor51HI AGCGCT 1 cut(s) 2350
AoxI GGCC 8 cut(s) 57, 124, 166, 1388, 2039, 2698, 2792, 2979
ApaLI GTGCAC 1 cut(s) 1709
ApeKI GCWGC 9 cut(s) 1169, 1181, 1184, 1187, 1190, 1451, 1497, 1652, 2453
ArsI GACNNNNNNTTYG 4 cut(s) 671, 701, 703, 733
AsiGI ACCGGT 1 cut(s) 1071
AsiSI GCGATCGC 1 cut(s) 1466
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 5 cut(s) 242, 1111, 1219, 2304, 2351
AspS9I GGNCC 4 cut(s) 124, 1388, 2792, 2813
AsuC2I CCSGG 1 cut(s) 1619
AsuHPI GGTGA 9 cut(s) 642, 888, 1415, 1595, 2202, 2393, 2597, 2894, 3302
AsuII TTCGAA 1 cut(s) 1645
AvaI CYCGRG 3 cut(s) 275, 847, 3409
AvaII GGWCC 1 cut(s) 2813
BaeGI GKGCMC 2 cut(s) 18, 1713
BaeI ACNNNNGTAYC 2 cut(s) 375, 408
BalI TGGCCA 2 cut(s) 59, 2041
BanI GGYRCC 1 cut(s) 3274
BanII GRGCYC 4 cut(s) 696, 996, 2158, 3410
BbsI GAAGAC 4 cut(s) 673, 739, 1280, 2772
Bbv12I GWGCWC 5 cut(s) 696, 996, 1713, 2562, 3410
BbvCI CCTCAGC 1 cut(s) 1119
BbvI GCAGC 9 cut(s) 1171, 1181, 1193, 1199, 1202, 1463, 1484, 1639, 2440
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 3 cut(s) 165, 1728, 2567
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 3 cut(s) 303, 2761, 2770
BcnI CCSGG 1 cut(s) 1619
BcoDI GTCTC 2 cut(s) 116, 1988
BfmI CTRYAG 4 cut(s) 1185, 1653, 2025, 2603
BfoI RGCGCY 1 cut(s) 2352
BfuI GTATCC 1 cut(s) 765
BglII AGATCT 1 cut(s) 2464
BisI GCNGC 9 cut(s) 1170, 1182, 1185, 1188, 1191, 1452, 1498, 1653, 2454
BlpI GCTNAGC 1 cut(s) 1692
BlsI GCNGC 9 cut(s) 1171, 1183, 1186, 1189, 1192, 1453, 1499, 1654, 2455
BmcAI AGTACT 2 cut(s) 727, 2205
Bme1390I CCNGG 4 cut(s) 165, 1619, 1728, 2567
Bme18I GGWCC 1 cut(s) 2813
BmeRI GACNNNNNGTC 1 cut(s) 3178
BmeT110I CYCGRG 3 cut(s) 275, 847, 3409
BmgT120I GGNCC 4 cut(s) 124, 1388, 2792, 2813
BmiI GGNNCC 2 cut(s) 2793, 3276
BmrFI CCNGG 4 cut(s) 165, 1619, 1728, 2567
BmrI ACTGGG 1 cut(s) 3369
BmsI GCATC 9 cut(s) 461, 605, 1420, 1438, 1801, 1917, 2004, 2434, 2625
BmuI ACTGGG 1 cut(s) 3369
BpiI GAAGAC 4 cut(s) 673, 739, 1280, 2772
BplI GAGNNNNNCTC 2 cut(s) 778, 810
BpmI CTGGAG 1 cut(s) 2348
Bpu10I CCTNAGC 3 cut(s) 983, 1119, 2136
Bpu1102I GCTNAGC 1 cut(s) 1692
Bpu14I TTCGAA 1 cut(s) 1645
BpuEI CTTGAG 5 cut(s) 980, 989, 1440, 2299, 3413
BpuMI CCSGG 1 cut(s) 1619
BsaAI YACGTR 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 5 cut(s) 120, 1606, 1617, 1628, 2436
BsaWI WCCGGW 2 cut(s) 1071, 1444
Bse118I RCCGGY 2 cut(s) 1071, 2981
Bse1I ACTGG 7 cut(s) 733, 1215, 1391, 1421, 2042, 2331, 3364
Bse3DI GCAATG 2 cut(s) 137, 544
BseAI TCCGGA 1 cut(s) 1444
BseBI CCWGG 3 cut(s) 165, 1728, 2567
BseDI CCNNGG 5 cut(s) 120, 1606, 1617, 1628, 2436
BseMI GCAATG 2 cut(s) 137, 544
BseMII CTCAG 8 cut(s) 825, 918, 997, 1110, 2127, 2336, 2632, 2775
BseNI ACTGG 7 cut(s) 733, 1215, 1391, 1421, 2042, 2331, 3364
BseRI GAGGAG 6 cut(s) 489, 991, 1240, 1340, 2296, 2801
BseSI GKGCMC 2 cut(s) 18, 1713
BseXI GCAGC 9 cut(s) 1171, 1181, 1193, 1199, 1202, 1463, 1484, 1639, 2440
BseYI CCCAGC 1 cut(s) 533
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 4 cut(s) 242, 1111, 2302, 2890
Bsh1285I CGRYCG 1 cut(s) 1466
BshFI GGCC 8 cut(s) 59, 126, 168, 1390, 2041, 2700, 2794, 2981
BshNI GGYRCC 1 cut(s) 3274
BshTI ACCGGT 1 cut(s) 1071
BsiEI CGRYCG 1 cut(s) 1466
BsiHKAI GWGCWC 5 cut(s) 696, 996, 1713, 2562, 3410
BsiHKCI CYCGRG 3 cut(s) 275, 847, 3409
BsiSI CCGG 5 cut(s) 169, 1072, 1445, 1619, 2982
BslFI GGGAC 1 cut(s) 3185
BsmAI GTCTC 2 cut(s) 116, 1988
BsmBI CGTCTC 1 cut(s) 1988
BsmFI GGGAC 1 cut(s) 3185
BsmI GAATGC 1 cut(s) 2753
BsnI GGCC 8 cut(s) 59, 126, 168, 1390, 2041, 2700, 2794, 2981
BsoBI CYCGRG 3 cut(s) 275, 847, 3409
Bsp119I TTCGAA 1 cut(s) 1645
Bsp1286I GDGCHC 7 cut(s) 18, 696, 996, 1713, 2158, 2562, 3410
Bsp13I TCCGGA 1 cut(s) 1444
Bsp1407I TGTACA 1 cut(s) 1014
Bsp1720I GCTNAGC 1 cut(s) 1692
Bsp19I CCATGG 1 cut(s) 2436
BspACI CCGC 5 cut(s) 488, 1814, 1984, 2193, 2519
BspANI GGCC 8 cut(s) 59, 126, 168, 1390, 2041, 2700, 2794, 2981
BspCNI CTCAG 8 cut(s) 824, 917, 996, 1111, 2128, 2337, 2631, 2776
BspEI TCCGGA 1 cut(s) 1444
BspFNI CGCG 4 cut(s) 242, 1111, 2302, 2890
BspLI GGNNCC 2 cut(s) 2793, 3276
BspMAI CTGCAG 4 cut(s) 1189, 1657, 2029, 2607
BspPI GGATC 4 cut(s) 191, 753, 1859, 2015
BspT104I TTCGAA 1 cut(s) 1645
BspT107I GGYRCC 1 cut(s) 3274
BsrBI CCGCTC 1 cut(s) 1984
BsrDI GCAATG 2 cut(s) 137, 544
BsrFI RCCGGY 2 cut(s) 1071, 2981
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 7 cut(s) 733, 1215, 1391, 1421, 2042, 2331, 3364
BssAI RCCGGY 2 cut(s) 1071, 2981
BssECI CCNNGG 5 cut(s) 120, 1606, 1617, 1628, 2436
BssNAI GTATAC 1 cut(s) 1396
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 3 cut(s) 120, 1628, 2436
Bst1107I GTATAC 1 cut(s) 1396
Bst2UI CCWGG 3 cut(s) 165, 1728, 2567
Bst4CI ACNGT 5 cut(s) 481, 1550, 1715, 1824, 2082
Bst6I CTCTTC 2 cut(s) 951, 1257
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 1661
BstAUI TGTACA 1 cut(s) 1014
BstBAI YACGTR 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 1645
BstC8I GCNNGC 7 cut(s) 629, 857, 1027, 1203, 1363, 1889, 1986
BstDSI CCRYGG 1 cut(s) 2436
BstENI CCTNNNNNAGG 1 cut(s) 1685
BstFNI CGCG 4 cut(s) 242, 1111, 2302, 2890
BstH2I RGCGCY 1 cut(s) 2352
BstHHI GCGC 5 cut(s) 242, 1111, 1219, 2304, 2351
BstMAI GTCTC 2 cut(s) 116, 1988
BstMCI CGRYCG 1 cut(s) 1466
BstNI CCWGG 3 cut(s) 165, 1728, 2567
BstNSI RCATGY 3 cut(s) 754, 1891, 3181
BstSCI CCNGG 4 cut(s) 163, 1617, 1726, 2565
BstSFI CTRYAG 4 cut(s) 1185, 1653, 2025, 2603
BstSLI GKGCMC 2 cut(s) 18, 1713
BstUI CGCG 4 cut(s) 242, 1111, 2302, 2890
BstV1I GCAGC 9 cut(s) 1171, 1181, 1193, 1199, 1202, 1463, 1484, 1639, 2440
BstV2I GAAGAC 4 cut(s) 673, 739, 1280, 2772
BstX2I RGATCY 2 cut(s) 196, 2464
BstYI RGATCY 2 cut(s) 196, 2464
BstZ17I GTATAC 1 cut(s) 1396
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 8 cut(s) 59, 126, 168, 1390, 2041, 2700, 2794, 2981
BtgI CCRYGG 1 cut(s) 2436
BtgZI GCGATG 3 cut(s) 427, 484, 3136
BtsI GCAGTG 3 cut(s) 54, 60, 406
BtsIMutI CAGTG 9 cut(s) 54, 60, 261, 299, 406, 1037, 2087, 2324, 2780
Cac8I GCNNGC 7 cut(s) 629, 857, 1027, 1203, 1363, 1889, 1986
CaiI CAGNNNCTG 1 cut(s) 2033
CfoI GCGC 5 cut(s) 242, 1111, 1219, 2304, 2351
Cfr10I RCCGGY 2 cut(s) 1071, 2981
Cfr13I GGNCC 4 cut(s) 124, 1388, 2792, 2813
CseI GACGC 3 cut(s) 29, 165, 424
Csp6I GTAC 9 cut(s) 341, 392, 726, 1015, 1306, 1315, 1546, 1567, 2204
CspAI ACCGGT 1 cut(s) 1071
CviQI GTAC 9 cut(s) 341, 392, 726, 1015, 1306, 1315, 1546, 1567, 2204
DraIII CACNNNGTG 1 cut(s) 1922
DriI GACNNNNNGTC 1 cut(s) 3178
EaeI YGGCCR 3 cut(s) 57, 166, 2039
Eam1104I CTCTTC 2 cut(s) 951, 1257
Eam1105I GACNNNNNGTC 1 cut(s) 3178
EarI CTCTTC 2 cut(s) 951, 1257
EciI GGCGGA 1 cut(s) 2208
Ecl136II GAGCTC 3 cut(s) 694, 994, 3408
Eco130I CCWWGG 3 cut(s) 120, 1628, 2436
Eco147I AGGCCT 1 cut(s) 2700
Eco24I GRGCYC 4 cut(s) 696, 996, 2158, 3410
Eco32I GATATC 1 cut(s) 1909
Eco47I GGWCC 1 cut(s) 2813
Eco47III AGCGCT 1 cut(s) 2350
Eco53kI GAGCTC 3 cut(s) 694, 994, 3408
Eco57I CTGAAG 4 cut(s) 285, 567, 1281, 2996
Eco88I CYCGRG 3 cut(s) 275, 847, 3409
EcoICRI GAGCTC 3 cut(s) 694, 994, 3408
EcoNI CCTNNNNNAGG 1 cut(s) 1685
EcoO109I RGGNCCY 1 cut(s) 2792
EcoRI GAATTC 2 cut(s) 2970, 3050
EcoRII CCWGG 3 cut(s) 163, 1726, 2565
EcoRV GATATC 1 cut(s) 1909
EcoT14I CCWWGG 3 cut(s) 120, 1628, 2436
EcoT38I GRGCYC 4 cut(s) 696, 996, 2158, 3410
ErhI CCWWGG 3 cut(s) 120, 1628, 2436
Esp3I CGTCTC 1 cut(s) 1988
FalI AAGNNNNNCTT 2 cut(s) 2818, 2850
FaqI GGGAC 1 cut(s) 3185
FauI CCCGC 1 cut(s) 1977
FauNDI CATATG 2 cut(s) 1294, 1830
FbaI TGATCA 3 cut(s) 303, 2761, 2770
FblI GTMKAC 2 cut(s) 1395, 2254
Fnu4HI GCNGC 9 cut(s) 1170, 1182, 1185, 1188, 1191, 1452, 1498, 1653, 2454
FriOI GRGCYC 4 cut(s) 696, 996, 2158, 3410
Fsp4HI GCNGC 9 cut(s) 1170, 1182, 1185, 1188, 1191, 1452, 1498, 1653, 2454
GlaI GCGC 5 cut(s) 241, 1110, 1218, 2303, 2350
GluI GCNGC 9 cut(s) 1170, 1182, 1185, 1188, 1191, 1452, 1498, 1653, 2454
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 2348
HaeII RGCGCY 1 cut(s) 2352
HaeIII GGCC 8 cut(s) 59, 126, 168, 1390, 2041, 2700, 2794, 2981
HapII CCGG 5 cut(s) 169, 1072, 1445, 1619, 2982
HgaI GACGC 3 cut(s) 29, 165, 424
HhaI GCGC 5 cut(s) 242, 1111, 1219, 2304, 2351
Hin1I GRCGYC 1 cut(s) 416
Hin6I GCGC 5 cut(s) 240, 1109, 1217, 2302, 2349
HinP1I GCGC 5 cut(s) 240, 1109, 1217, 2302, 2349
HincII GTYRAC 2 cut(s) 180, 2255
HindII GTYRAC 2 cut(s) 180, 2255
HindIII AAGCTT 2 cut(s) 2002, 2610
HinfI GANTC 9 cut(s) 187, 375, 557, 779, 1256, 1969, 2222, 2284, 2885
HpaII CCGG 5 cut(s) 169, 1072, 1445, 1619, 2982
HphI GGTGA 9 cut(s) 642, 888, 1415, 1595, 2202, 2393, 2597, 2894, 3302
Hpy166II GTNNAC 8 cut(s) 180, 1317, 1396, 1406, 1711, 2255, 2642, 3233
Hpy8I GTNNAC 8 cut(s) 180, 1317, 1396, 1406, 1711, 2255, 2642, 3233
Hpy99I CGWCG 3 cut(s) 23, 801, 2168
HpyCH4III ACNGT 5 cut(s) 481, 1550, 1715, 1824, 2082
HpyCH4IV ACGT 4 cut(s) 505, 799, 1441, 3229
HpySE526I ACGT 4 cut(s) 505, 799, 1441, 3229
Hsp92I GRCGYC 1 cut(s) 416
HspAI GCGC 5 cut(s) 240, 1109, 1217, 2302, 2349
Kpn2I TCCGGA 1 cut(s) 1444
Ksp22I TGATCA 3 cut(s) 303, 2761, 2770
LmnI GCTCC 7 cut(s) 147, 479, 699, 964, 1532, 1581, 2356
Lsp1109I GCAGC 9 cut(s) 1171, 1181, 1193, 1199, 1202, 1463, 1484, 1639, 2440
LweI GCATC 9 cut(s) 461, 605, 1420, 1438, 1801, 1917, 2004, 2434, 2625
MaeII ACGT 4 cut(s) 505, 799, 1441, 3229
MaeIII GTNAC 9 cut(s) 648, 707, 930, 1610, 1715, 1816, 3091, 3109, 3299
MbiI CCGCTC 1 cut(s) 1984
MfeI CAATTG 4 cut(s) 327, 1320, 3068, 3215
MflI RGATCY 2 cut(s) 196, 2464
MhlI GDGCHC 7 cut(s) 18, 696, 996, 1713, 2158, 2562, 3410
MlsI TGGCCA 2 cut(s) 59, 2041
MluNI TGGCCA 2 cut(s) 59, 2041
MlyI GAGTC 3 cut(s) 1963, 2216, 2293
MmeI TCCRAC 3 cut(s) 377, 867, 2980
Mox20I TGGCCA 2 cut(s) 59, 2041
MroI TCCGGA 1 cut(s) 1444
MroXI GAANNNNTTC 1 cut(s) 670
MscI TGGCCA 2 cut(s) 59, 2041
MseI TTAA 8 cut(s) 35, 116, 315, 1068, 1251, 1721, 2142, 3330
MslI CAYNNNNRTG 1 cut(s) 3311
Msp20I TGGCCA 2 cut(s) 59, 2041
MspA1I CMGCKG 2 cut(s) 1184, 1844
MspI CCGG 5 cut(s) 169, 1072, 1445, 1619, 2982
MspR9I CCNGG 4 cut(s) 165, 1619, 1728, 2567
MunI CAATTG 4 cut(s) 327, 1320, 3068, 3215
Mva1269I GAATGC 1 cut(s) 2753
MvaI CCWGG 3 cut(s) 165, 1728, 2567
MvnI CGCG 4 cut(s) 242, 1111, 2302, 2890
NciI CCSGG 1 cut(s) 1619
NcoI CCATGG 1 cut(s) 2436
NdeI CATATG 2 cut(s) 1294, 1830
NlaIV GGNNCC 2 cut(s) 2793, 3276
NmuCI GTSAC 6 cut(s) 648, 707, 930, 1715, 1816, 3299
NspI RCATGY 3 cut(s) 754, 1891, 3181
NspV TTCGAA 1 cut(s) 1645
PaeI GCATGC 1 cut(s) 1891
PaeR7I CTCGAG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 2700
PctI GAATGC 1 cut(s) 2753
PdmI GAANNNNTTC 1 cut(s) 670
PfeI GAWTC 6 cut(s) 187, 375, 557, 779, 1256, 2885
PflFI GACNNNGTC 1 cut(s) 175
PflMI CCANNNNNTGG 4 cut(s) 164, 2264, 2326, 2822
PinAI ACCGGT 1 cut(s) 1071
PkrI GCNGC 9 cut(s) 1171, 1183, 1186, 1189, 1192, 1453, 1499, 1654, 2455
Ple19I CGATCG 1 cut(s) 1466
PleI GAGTC 3 cut(s) 1963, 2216, 2292
PpsI GAGTC 3 cut(s) 1963, 2216, 2292
Ppu21I YACGTR 1 cut(s) 506
Psp124BI GAGCTC 3 cut(s) 696, 996, 3410
Psp6I CCWGG 3 cut(s) 163, 1726, 2565
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 3 cut(s) 163, 1726, 2565
PspN4I GGNNCC 2 cut(s) 2793, 3276
PspPI GGNCC 4 cut(s) 124, 1388, 2792, 2813
PspXI VCTCGAGB 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 333, 365
PstI CTGCAG 4 cut(s) 1189, 1657, 2029, 2607
PstNI CAGNNNCTG 1 cut(s) 2033
PsuI RGATCY 2 cut(s) 196, 2464
PsyI GACNNNGTC 1 cut(s) 175
PvuI CGATCG 1 cut(s) 1466
PvuII CAGCTG 2 cut(s) 1184, 1844
RgaI GCGATCGC 1 cut(s) 1466
RsaI GTAC 9 cut(s) 342, 393, 727, 1016, 1307, 1316, 1547, 1568, 2205
RsaNI GTAC 9 cut(s) 341, 392, 726, 1015, 1306, 1315, 1546, 1567, 2204
RseI CAYNNNNRTG 1 cut(s) 3311
SacI GAGCTC 3 cut(s) 696, 996, 3410
SalI GTCGAC 1 cut(s) 2253
SaqAI TTAA 8 cut(s) 35, 116, 315, 1068, 1251, 1721, 2142, 3330
SatI GCNGC 9 cut(s) 1170, 1182, 1185, 1188, 1191, 1452, 1498, 1653, 2454
Sau96I GGNCC 4 cut(s) 124, 1388, 2792, 2813
ScaI AGTACT 2 cut(s) 727, 2205
SchI GAGTC 3 cut(s) 1963, 2216, 2293
ScrFI CCNGG 4 cut(s) 165, 1619, 1728, 2567
SduI GDGCHC 7 cut(s) 18, 696, 996, 1713, 2158, 2562, 3410
SfaAI GCGATCGC 1 cut(s) 1466
SfaNI GCATC 9 cut(s) 461, 605, 1420, 1438, 1801, 1917, 2004, 2434, 2625
SfcI CTRYAG 4 cut(s) 1185, 1653, 2025, 2603
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 1645
SgfI GCGATCGC 1 cut(s) 1466
SinI GGWCC 1 cut(s) 2813
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 3311
SmlI CTYRAG 6 cut(s) 275, 968, 995, 1455, 2278, 3392
SmoI CTYRAG 6 cut(s) 275, 968, 995, 1455, 2278, 3392
SphI GCATGC 1 cut(s) 1891
SseBI AGGCCT 1 cut(s) 2700
SsiI CCGC 5 cut(s) 488, 1814, 1984, 2193, 2519
SstI GAGCTC 3 cut(s) 696, 996, 3410
StuI AGGCCT 1 cut(s) 2700
StyD4I CCNGG 4 cut(s) 163, 1617, 1726, 2565
StyI CCWWGG 3 cut(s) 120, 1628, 2436
TaaI ACNGT 5 cut(s) 481, 1550, 1715, 1824, 2082
TaiI ACGT 4 cut(s) 508, 802, 1444, 3232
TaqI TCGA 8 cut(s) 276, 674, 796, 1645, 2254, 2469, 2903, 3281
TaqII GACCGA 1 cut(s) 2121
TatI WGTACW 5 cut(s) 725, 1014, 1314, 1566, 2203
TfiI GAWTC 6 cut(s) 187, 375, 557, 779, 1256, 2885
Tru1I TTAA 8 cut(s) 35, 116, 315, 1068, 1251, 1721, 2142, 3330
Tru9I TTAA 8 cut(s) 35, 116, 315, 1068, 1251, 1721, 2142, 3330
TscAI CASTG 9 cut(s) 54, 60, 268, 306, 406, 1044, 2087, 2331, 2780
TseFI GTSAC 6 cut(s) 648, 707, 930, 1715, 1816, 3299
TseI GCWGC 9 cut(s) 1169, 1181, 1184, 1187, 1190, 1451, 1497, 1652, 2453
Tsp45I GTSAC 6 cut(s) 648, 707, 930, 1715, 1816, 3299
TspDTI ATGAA 7 cut(s) 556, 654, 1077, 1256, 1673, 2704, 3240
TspGWI ACGGA 2 cut(s) 197, 833
TspRI CASTG 9 cut(s) 54, 60, 268, 306, 406, 1044, 2087, 2331, 2780
Tth111I GACNNNGTC 1 cut(s) 175
Van91I CCANNNNNTGG 4 cut(s) 164, 2264, 2326, 2822
VneI GTGCAC 1 cut(s) 1709
VpaK11BI GGWCC 1 cut(s) 2813
XagI CCTNNNNNAGG 1 cut(s) 1685
XceI RCATGY 3 cut(s) 754, 1891, 3181
XcmI CCANNNNNNNNNTGG 1 cut(s) 1957
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 2 cut(s) 1395, 2254
XmnI GAANNNNTTC 1 cut(s) 670
ZrmI AGTACT 2 cut(s) 727, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.