RLG00000019446

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000353
Physical Location & Seq
Forward (+)
52106987 .. 52111139
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019446

Sequence Viewer

Length: 2277 bp
ATGGGAATTAGAGGGCAATCAACAGCAGAAAGGAGAGAAAAGACAGTGGTCCTGGCAATAGACAAGGACAAAGGAAGTCAATATGCTATCAAATGGGTTGTTGATCAATTTCTTACCAGGGGCCAAGCTCTCACATTGCTCCATGTTACATGCAAAACAAATCCTGCTCCCAACCAAACTTTGTCTGCAGCTGGGGGGACGGTTTTGATCTGTGACGTGACACACGACTCTCAATCCGAAGAGATGTTTCTTCCATTCCGCGCTTATTGCTCAAAAAGGAATATAATATGCAATGAAGTCACAGTTAAGGATACAGATGTCCCAAAAGCGTTGATCAAATACGTTGTGGCCAATTCAATTGAGATTTTGGTACTTGGTTCACCATCAAGATCCGGCCTTTTCAAATTCAGAACCACAGATGTTCCAAGCATTGTGTTGAAAGGGGCACCGGATTTTTGCAGTGTGTATGTAGTTGGCAAAGGAAAGCTCTCAAATGTCCGAACAGCTACCAGTACACCACGACTAAAAGCTGCTCCCTCATGTAATCAAATGCAGCACCAACCCTGCAAAGTTTTTGAATCAAATGATACACGAACGATGAGCTACCGGCAAGCTAGAGAATCAGAGAGCACACTTTACCACCCCCCTAGCAAGCCAGATGACTTTGAAATCAAGTCACCATTCACAAGAGGAAACTTGGCAATGAACAAATCATATCAGCTCTCACCAGAAACTGATATATCATACGTCGGTAGTGGCAAGCCAAACATGGAGAACATGTTTCCTTCTTTCTATGATGGTTTAGGATCAGGAATTAGCCCTCATTATTCAACCAACTCAGAGTGGGAGAACAGAAGCTCCACGTCGACATCTTACTCAGGAACTAAGTTTATTGACATGAGCTCAGCACAAAATGATTTTTCCTCAGCTTCAATTGAAAGTCGAAAGTCCTGTCACTCACAGAATATGGATGAAGAAATGGAAGCTGAGATGAGGAAGCTGAGGCTAGAGCTCAAGCAAACAATGGACATGTACAATACCGTCTGCAAGGAGGCTCTCTCTCCAGAACACAAGGACATGGAACTTAACCGATGGAAATTGGAAGAAAAAAAGAGATTAGAAGAGGCACAACTAGCTGAGGAAGTTGCATTGGCACTTGTACAGAGAGAAAAAGCGAATCGTAAGGCAGCCATTGAGGCAGCAGAAGCAGCTCAAAGGATTGCCGAATGGGAATCACAAAAAAGGAGGAATGCAGAACTGAAAGCCCTTAAAGAGGCTAAAGAGAGAAAGAAGGCATCAGAAGCAAGGGCCTGTGATATCAGTTATAGAAAATACACAATTGAGGAAATTGAATCAGCAACAAATAATTTTTCGATATCTTGCAAGATTGGAGAAGGAGGTTATGGTCCAGTTTACAAAGGGGAACTGGACCACACACCAGTGGCGATAAAAGCTCTGCGTCCAGATGCAGCTCAAGGACAGTCACAGTTCCAGCAAGAGGTTAAACTATTGAGTGGCATGCGACATCCGAACATGGTTCTCCTTCTTGGAGCCTGTCCAGATTATGGTTGCCTGATTTATGAGTACATGGCTAATGGGAGCTTAGAAGAACGTATCTTCAAGCGAGGTAACACCCCAGTTATTCCTTGGCAACTAAGGTTCAGAATCGCTGCTGAAATCTGCACTGGGCTTTTGTTTCTTCATCAAACCAAGCCAGAGCCCCTAGTACACCGTGACCTCAAACCCGCTAACATTTTGCTAGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTTTGGCCAGACTTGTCCCTCCATCAGTAGCTGATTCTATCACTCAGTATCGCATTACATCAGCAGCCGGAACATCCTGTTATATTGATCCTGAGTATCAGCAAACTGGTATGCTGGGAAGAAAATCTGATGTCTACTCACTAGGGGTTATGCTTTTACAACTAATCACAGCCAAGTCACCAATAGGTTTGACGTATCATGTTGAGAAAGCTATTAAGGAAGGGACTTTCACTGAGATGCTTGACCCTGTTGTTCCTGACTGGCCAGTTGAGGAAACCTTAGAGTTTGCCAGGCTAGCTTTACAGTGTGCTCAAATGAAACGAAAAGACAGACCGGATCTTGGGAAGGTTTTGCTACCTGAGTTTAACAGATTGAGAGCATTTGCCGAAGATAGCATGAATTCTGTCATGCGGGGTGGTGGTAAAGAACTCTTGTCAAGACAAGACCCGATTTCCACAAGACAAGTAAGTTGCATAACATAA

Protein Analysis

759

Amino Acids

84.8

Weight (kDa)

8.27

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 14 - 158 1.4e-10 Universal stress protein family
PK_Tyr_Ser-Thr PF07714 459 - 715 2.8e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 460 - 714 5.1e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 445
AccB7I CCANNNNNTGG 1 cut(s) 1565
AccI GTMKAC 2 cut(s) 866, 1929
AccII CGCG 1 cut(s) 261
AciI CCGC 3 cut(s) 259, 1746, 2206
AclWI GGATC 4 cut(s) 384, 814, 1877, 2139
AcoI YGGCCR 3 cut(s) 348, 1800, 2057
AcsI RAATTY 2 cut(s) 404, 2194
AfaI GTAC 6 cut(s) 372, 514, 1034, 1161, 1586, 1728
AfiI CCNNNNNNNGG 4 cut(s) 1273, 1498, 1565, 2135
AflIII ACRYGT 2 cut(s) 777, 1029
AjiI CACGTC 2 cut(s) 217, 864
AjnI CCWGG 3 cut(s) 51, 116, 2084
AleI CACNNNNGTG 1 cut(s) 1439
AloI GAACNNNNNNTCC 2 cut(s) 842, 874
Alw21I GWGCWC 4 cut(s) 632, 905, 1014, 2107
AlwI GGATC 4 cut(s) 384, 814, 1877, 2139
AlwNI CAGNNNCTG 2 cut(s) 734, 1826
AoxI GGCC 6 cut(s) 121, 348, 394, 1308, 1800, 2057
ApeKI GCWGC 9 cut(s) 188, 530, 553, 1187, 1199, 1208, 1469, 1670, 1859
ApoI RAATTY 2 cut(s) 404, 2194
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 5 cut(s) 49, 121, 1308, 1406, 1429
AsuHPI GGTGA 4 cut(s) 372, 669, 717, 1965
AsuNHI GCTAGC 1 cut(s) 2089
AvaII GGWCC 3 cut(s) 49, 1406, 1429
BaeGI GKGCMC 1 cut(s) 448
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BalI TGGCCA 3 cut(s) 350, 1802, 2059
BanI GGYRCC 1 cut(s) 445
BanII GRGCYC 3 cut(s) 905, 1014, 1722
Bbv12I GWGCWC 4 cut(s) 632, 905, 1014, 2107
BbvCI CCTCAGC 3 cut(s) 925, 1001, 1137
BbvI GCAGC 9 cut(s) 200, 517, 565, 1199, 1211, 1220, 1481, 1657, 1871
BccI CCATC 4 cut(s) 391, 791, 1086, 1825
BciT130I CCWGG 3 cut(s) 53, 118, 2086
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 2 cut(s) 103, 333
BfaI CTAG 8 cut(s) 615, 648, 1007, 1133, 1724, 1760, 1937, 2090
BfmI CTRYAG 1 cut(s) 186
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 1196
BisI GCNGC 9 cut(s) 189, 531, 554, 1188, 1200, 1209, 1470, 1671, 1860
BlpI GCTNAGC 1 cut(s) 904
BlsI GCNGC 9 cut(s) 190, 532, 555, 1189, 1201, 1210, 1471, 1672, 1861
Bme1390I CCNGG 3 cut(s) 53, 118, 2086
Bme18I GGWCC 3 cut(s) 49, 1406, 1429
BmgBI CACGTC 2 cut(s) 217, 864
BmgT120I GGNCC 5 cut(s) 49, 121, 1308, 1406, 1429
BmiI GGNNCC 3 cut(s) 122, 447, 1552
BmrFI CCNGG 3 cut(s) 53, 118, 2086
BmrI ACTGGG 2 cut(s) 1631, 1695
BmsI GCATC 3 cut(s) 1304, 1456, 2022
BmtI GCTAGC 1 cut(s) 2093
BmuI ACTGGG 2 cut(s) 1631, 1695
BoxI GACNNNNGTC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 1 cut(s) 1047
Bpu10I CCTNAGC 3 cut(s) 925, 1001, 1137
Bpu1102I GCTNAGC 1 cut(s) 904
BpuEI CTTGAG 2 cut(s) 998, 1458
BsaJI CCNNGG 2 cut(s) 117, 1646
BsaWI WCCGGW 2 cut(s) 448, 2128
BsaXI ACNNNNNCTCC 4 cut(s) 764, 794, 842, 872
Bsc4I CCNNNNNNNGG 4 cut(s) 1273, 1498, 1565, 2135
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 9 cut(s) 510, 1409, 1431, 1439, 1637, 1690, 1906, 2060, 2060
Bse3DI GCAATG 3 cut(s) 134, 298, 708
BseBI CCWGG 3 cut(s) 53, 118, 2086
BseDI CCNNGG 2 cut(s) 117, 1646
BseGI GGATG 3 cut(s) 976, 1525, 1868
BseLI CCNNNNNNNGG 4 cut(s) 1273, 1498, 1565, 2135
BseMI GCAATG 3 cut(s) 134, 298, 708
BseNI ACTGG 9 cut(s) 510, 1409, 1431, 1439, 1637, 1690, 1906, 2060, 2060
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 9 cut(s) 200, 517, 565, 1199, 1211, 1220, 1481, 1657, 1871
BseYI CCCAGC 2 cut(s) 191, 1909
BsgI GTGCAG 1 cut(s) 1666
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 6 cut(s) 123, 350, 396, 1310, 1802, 2059
BshNI GGYRCC 1 cut(s) 445
BsiHKAI GWGCWC 4 cut(s) 632, 905, 1014, 2107
BsiSI CCGG 5 cut(s) 393, 449, 607, 1863, 2129
BslFI GGGAC 4 cut(s) 211, 305, 1796, 2032
BslI CCNNNNNNNGG 4 cut(s) 1273, 1498, 1565, 2135
BsmFI GGGAC 4 cut(s) 211, 305, 1796, 2032
BsmI GAATGC 1 cut(s) 1255
BsnI GGCC 6 cut(s) 123, 350, 396, 1310, 1802, 2059
Bsp1286I GDGCHC 6 cut(s) 448, 632, 905, 1014, 1722, 2107
Bsp1407I TGTACA 2 cut(s) 1032, 1159
Bsp143I GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
Bsp1720I GCTNAGC 1 cut(s) 904
BspACI CCGC 3 cut(s) 259, 1746, 2206
BspANI GGCC 6 cut(s) 123, 350, 396, 1310, 1802, 2059
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 122, 447, 1552
BspMAI CTGCAG 1 cut(s) 190
BspOI GCTAGC 1 cut(s) 2093
BspPI GGATC 4 cut(s) 384, 814, 1877, 2139
BspT107I GGYRCC 1 cut(s) 445
BsrDI GCAATG 3 cut(s) 134, 298, 708
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 2 cut(s) 1032, 1159
BsrI ACTGG 9 cut(s) 510, 1409, 1431, 1439, 1637, 1690, 1906, 2060, 2060
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 117, 1646
BssMI GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
BssT1I CCWWGG 1 cut(s) 1646
Bst2UI CCWGG 3 cut(s) 53, 118, 2086
Bst4CI ACNGT 8 cut(s) 46, 202, 304, 1042, 1482, 1488, 1733, 2100
Bst6I CTCTTC 2 cut(s) 234, 1116
BstAUI TGTACA 2 cut(s) 1032, 1159
BstC8I GCNNGC 5 cut(s) 612, 653, 761, 1520, 2091
BstENI CCTNNNNNAGG 1 cut(s) 1271
BstF5I GGATG 3 cut(s) 976, 1525, 1868
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 7 cut(s) 106, 210, 336, 392, 809, 1885, 2134
BstMBI GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
BstMWI GCNNNNNNNGC 8 cut(s) 267, 1133, 1196, 1205, 1208, 1301, 1451, 2090
BstNI CCWGG 3 cut(s) 53, 118, 2086
BstNSI RCATGY 4 cut(s) 153, 781, 1033, 1522
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 3 cut(s) 51, 116, 2084
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 9 cut(s) 200, 517, 565, 1199, 1211, 1220, 1481, 1657, 1871
BstX2I RGATCY 2 cut(s) 389, 2131
BstYI RGATCY 2 cut(s) 389, 2131
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 6 cut(s) 123, 350, 396, 1310, 1802, 2059
BtrI CACGTC 2 cut(s) 217, 864
BtsCI GGATG 3 cut(s) 976, 1525, 1868
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 6 cut(s) 51, 466, 1446, 1683, 2025, 2105
Cac8I GCNNGC 5 cut(s) 612, 653, 761, 1520, 2091
CaiI CAGNNNCTG 2 cut(s) 734, 1826
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 5 cut(s) 49, 121, 1308, 1406, 1429
CseI GACGC 1 cut(s) 1448
Csp6I GTAC 6 cut(s) 371, 513, 1033, 1160, 1585, 1727
CviQI GTAC 6 cut(s) 371, 513, 1033, 1160, 1585, 1727
DpnI GATC 7 cut(s) 105, 209, 335, 391, 808, 1884, 2133
DpnII GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
EaeI YGGCCR 3 cut(s) 348, 1800, 2057
Eam1104I CTCTTC 2 cut(s) 234, 1116
EarI CTCTTC 2 cut(s) 234, 1116
Ecl136II GAGCTC 2 cut(s) 903, 1012
Eco130I CCWWGG 1 cut(s) 1646
Eco24I GRGCYC 3 cut(s) 905, 1014, 1722
Eco32I GATATC 2 cut(s) 1318, 1377
Eco47I GGWCC 3 cut(s) 49, 1406, 1429
Eco53kI GAGCTC 2 cut(s) 903, 1012
EcoICRI GAGCTC 2 cut(s) 903, 1012
EcoNI CCTNNNNNAGG 1 cut(s) 1271
EcoO109I RGGNCCY 1 cut(s) 1308
EcoRI GAATTC 1 cut(s) 2194
EcoRII CCWGG 3 cut(s) 51, 116, 2084
EcoRV GATATC 2 cut(s) 1318, 1377
EcoT14I CCWWGG 1 cut(s) 1646
EcoT38I GRGCYC 3 cut(s) 905, 1014, 1722
ErhI CCWWGG 1 cut(s) 1646
FaqI GGGAC 4 cut(s) 211, 305, 1796, 2032
FauI CCCGC 2 cut(s) 1753, 2199
FbaI TGATCA 2 cut(s) 103, 333
FblI GTMKAC 2 cut(s) 866, 1929
Fnu4HI GCNGC 9 cut(s) 189, 531, 554, 1188, 1200, 1209, 1470, 1671, 1860
FokI GGATG 3 cut(s) 983, 1512, 1855
FriOI GRGCYC 3 cut(s) 905, 1014, 1722
Fsp4HI GCNGC 9 cut(s) 189, 531, 554, 1188, 1200, 1209, 1470, 1671, 1860
FspBI CTAG 8 cut(s) 615, 648, 1007, 1133, 1724, 1760, 1937, 2090
GlaI GCGC 1 cut(s) 262
GluI GCNGC 9 cut(s) 189, 531, 554, 1188, 1200, 1209, 1470, 1671, 1860
GsaI CCCAGC 2 cut(s) 195, 1913
GsuI CTGGAG 1 cut(s) 1047
HaeIII GGCC 6 cut(s) 123, 350, 396, 1310, 1802, 2059
HapII CCGG 5 cut(s) 393, 449, 607, 1863, 2129
HgaI GACGC 1 cut(s) 1448
HhaI GCGC 1 cut(s) 263
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HinfI GANTC 8 cut(s) 227, 578, 620, 1177, 1232, 1352, 1665, 1829
HpaII CCGG 5 cut(s) 393, 449, 607, 1863, 2129
HphI GGTGA 4 cut(s) 372, 669, 717, 1965
Hpy166II GTNNAC 6 cut(s) 380, 515, 867, 1414, 1729, 1930
Hpy188I TCNGA 9 cut(s) 238, 410, 500, 625, 841, 1300, 1530, 1664, 1924
Hpy188III TCNNGA 9 cut(s) 387, 810, 879, 1064, 1463, 1559, 1886, 2051, 2232
Hpy8I GTNNAC 6 cut(s) 380, 515, 867, 1414, 1729, 1930
Hpy99I CGWCG 2 cut(s) 752, 868
HpyAV CCTTC 6 cut(s) 795, 1285, 1388, 1553, 2009, 2134
HpyCH4III ACNGT 8 cut(s) 46, 202, 304, 1042, 1482, 1488, 1733, 2100
HpyCH4IV ACGT 6 cut(s) 216, 342, 747, 863, 1612, 1988
HpyF10VI GCNNNNNNNGC 8 cut(s) 267, 1133, 1196, 1205, 1208, 1301, 1451, 2090
HpySE526I ACGT 6 cut(s) 216, 342, 747, 863, 1612, 1988
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 2 cut(s) 103, 333
Kzo9I GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
LmnI GCTCC 6 cut(s) 144, 172, 538, 863, 1550, 1599
Lsp1109I GCAGC 9 cut(s) 200, 517, 565, 1199, 1211, 1220, 1481, 1657, 1871
LweI GCATC 3 cut(s) 1304, 1456, 2022
MaeI CTAG 8 cut(s) 615, 648, 1007, 1133, 1724, 1760, 1937, 2090
MaeII ACGT 6 cut(s) 216, 342, 747, 863, 1612, 1988
MalI GATC 7 cut(s) 105, 209, 335, 391, 808, 1884, 2133
MboI GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
MfeI CAATTG 3 cut(s) 357, 933, 1338
MflI RGATCY 2 cut(s) 389, 2131
MhlI GDGCHC 6 cut(s) 448, 632, 905, 1014, 1722, 2107
MlsI TGGCCA 3 cut(s) 350, 1802, 2059
MluNI TGGCCA 3 cut(s) 350, 1802, 2059
MlyI GAGTC 1 cut(s) 221
Mox20I TGGCCA 3 cut(s) 350, 1802, 2059
MscI TGGCCA 3 cut(s) 350, 1802, 2059
MseI TTAA 6 cut(s) 306, 1086, 1269, 1503, 2010, 2160
MslI CAYNNNNRTG 3 cut(s) 1439, 1770, 2030
Msp20I TGGCCA 3 cut(s) 350, 1802, 2059
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 5 cut(s) 393, 449, 607, 1863, 2129
MspR9I CCNGG 3 cut(s) 53, 118, 2086
MunI CAATTG 3 cut(s) 357, 933, 1338
Mva1269I GAATGC 1 cut(s) 1255
MvaI CCWGG 3 cut(s) 53, 118, 2086
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 8 cut(s) 267, 1133, 1196, 1205, 1208, 1301, 1451, 2090
NdeII GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
NheI GCTAGC 1 cut(s) 2089
NlaIV GGNNCC 3 cut(s) 122, 447, 1552
NmuCI GTSAC 8 cut(s) 212, 217, 298, 675, 953, 1482, 1733, 1971
NspI RCATGY 4 cut(s) 153, 781, 1033, 1522
OliI CACNNNNGTG 1 cut(s) 1439
PaeI GCATGC 1 cut(s) 1522
PciI ACATGT 2 cut(s) 777, 1029
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 1255
PfeI GAWTC 7 cut(s) 578, 620, 1177, 1232, 1352, 1665, 1829
PflMI CCANNNNNTGG 1 cut(s) 1565
PkrI GCNGC 9 cut(s) 190, 532, 555, 1189, 1201, 1210, 1471, 1672, 1861
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PscI ACATGT 2 cut(s) 777, 1029
PshAI GACNNNNGTC 1 cut(s) 47
Psp124BI GAGCTC 2 cut(s) 905, 1014
Psp6I CCWGG 3 cut(s) 51, 116, 2084
PspFI CCCAGC 2 cut(s) 191, 1909
PspGI CCWGG 3 cut(s) 51, 116, 2084
PspN4I GGNNCC 3 cut(s) 122, 447, 1552
PspPI GGNCC 5 cut(s) 49, 121, 1308, 1406, 1429
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 2 cut(s) 734, 1826
PsuI RGATCY 2 cut(s) 389, 2131
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 6 cut(s) 372, 514, 1034, 1161, 1586, 1728
RsaNI GTAC 6 cut(s) 371, 513, 1033, 1160, 1585, 1727
RseI CAYNNNNRTG 3 cut(s) 1439, 1770, 2030
SacI GAGCTC 2 cut(s) 905, 1014
SalI GTCGAC 1 cut(s) 865
SaqAI TTAA 6 cut(s) 306, 1086, 1269, 1503, 2010, 2160
SatI GCNGC 9 cut(s) 189, 531, 554, 1188, 1200, 1209, 1470, 1671, 1860
Sau3AI GATC 7 cut(s) 103, 207, 333, 389, 806, 1882, 2131
Sau96I GGNCC 5 cut(s) 49, 121, 1308, 1406, 1429
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 53, 118, 2086
SduI GDGCHC 6 cut(s) 448, 632, 905, 1014, 1722, 2107
SfaNI GCATC 3 cut(s) 1304, 1456, 2022
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 3 cut(s) 49, 1406, 1429
SmiMI CAYNNNNRTG 3 cut(s) 1439, 1770, 2030
SmlI CTYRAG 2 cut(s) 1013, 1473
SmoI CTYRAG 2 cut(s) 1013, 1473
SphI GCATGC 1 cut(s) 1522
SsiI CCGC 3 cut(s) 259, 1746, 2206
SspMI CTAG 8 cut(s) 615, 648, 1007, 1133, 1724, 1760, 1937, 2090
SstI GAGCTC 2 cut(s) 905, 1014
StyD4I CCNGG 3 cut(s) 51, 116, 2084
StyI CCWWGG 1 cut(s) 1646
TaaI ACNGT 8 cut(s) 46, 202, 304, 1042, 1482, 1488, 1733, 2100
TaiI ACGT 6 cut(s) 219, 345, 750, 866, 1615, 1991
TaqI TCGA 3 cut(s) 866, 943, 1373
TatI WGTACW 5 cut(s) 512, 1032, 1159, 1584, 1726
TfiI GAWTC 7 cut(s) 578, 620, 1177, 1232, 1352, 1665, 1829
Tru1I TTAA 6 cut(s) 306, 1086, 1269, 1503, 2010, 2160
Tru9I TTAA 6 cut(s) 306, 1086, 1269, 1503, 2010, 2160
TscAI CASTG 6 cut(s) 51, 466, 1446, 1690, 2032, 2105
TseFI GTSAC 8 cut(s) 212, 217, 298, 675, 953, 1482, 1733, 1971
TseI GCWGC 9 cut(s) 188, 530, 553, 1187, 1199, 1208, 1469, 1670, 1859
Tsp45I GTSAC 8 cut(s) 212, 217, 298, 675, 953, 1482, 1733, 1971
TspDTI ATGAA 6 cut(s) 309, 719, 987, 1691, 2126, 2207
TspRI CASTG 6 cut(s) 51, 466, 1446, 1690, 2032, 2105
Van91I CCANNNNNTGG 1 cut(s) 1565
VpaK11BI GGWCC 3 cut(s) 49, 1406, 1429
XagI CCTNNNNNAGG 1 cut(s) 1271
XapI RAATTY 2 cut(s) 404, 2194
XceI RCATGY 4 cut(s) 153, 781, 1033, 1522
XcmI CCANNNNNNNNNTGG 1 cut(s) 1644
XmiI GTMKAC 2 cut(s) 866, 1929
XspI CTAG 8 cut(s) 615, 648, 1007, 1133, 1724, 1760, 1937, 2090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.