Rroxscaffold_2G00109420

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000353
Physical Location & Seq
Reverse (-)
33611369 .. 33615777
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109420.1

Sequence Viewer

Length: 2136 bp
ATGGGAATTAGAGGGCAATCAACAGCAGAAAGGAGAGAAAAGACAGTGGCCCTGGCAATAGACAAGGACAAAGGAAGTCAATATGCTATCAAATGGGTTGTTGATCAATTTCTTACCAAGGGCCAAGCTCTCACATTGCTCCATGTTACACGCAAAACAAATCCTGCTCCCTACCAAACCTTGTCTGCAGCTGGGGAGATGGTTTTGATCTGTGACATGACACACGACTCTCAATCCGAAGAGATGTTTCTTCCATTCCGCGCTTATTGCTCAAAAAGGAATATAATATGCAATGAAGTCACAGTTGAGGATACAGATGTCCCAAAAGCATTGATCAAATACGTTGTGGCCAATTCAATTGAGATTTTGGTACTTGGTTCACCATCAAGATCCGGCCTTTTCAAAAGATTCAGAACCACAGATGTTCCAAGCATTGTGTTGAAAGGGGCACCGGATTTTTGCAGTGTTTATGTAGTTGGCAAAGGAAAGCTCTCAAATGTCCGAACAGCTACCAGTACACCACGACTAAAAGCTGCTCCCTCATGTAATCAAATGCAGCACCAACCCTGCAAAGTTTCTGAATCAAATGATACACGAACGATGAGCTACCGGCAAGCTAGAGAATCAGAGAGCACACGTTACCACCGCCCTAGCAAGCCAGATGACTTTGAAATCAAGTCACCATTCACAAGAGGAAATTTGGCAATGAACAAATCATATCAGCTCTCACCAGAAACTGATATATCATACGTCGGTAGTGGCAAGCCAAACATGGATAACATGTTTCCTTCTTTCTATGATGGTTTAGAGTCAGGAATTAGCCCTCAATATTCAACCAACTCAGAGTGGGAGAACAGAAGCTCCACGTCGACATCTTACTCAGGAACTAAGTTTATTGACATGAGCTCAGCACAAAATGAATTTTCCTCAGCTTCAATTGAAAGTCGAAAGTCCTGTCACTCACAGAATGTGGATGAAGAAATGGAGGCTGAGATGAGGAAGCTGAGGCTAGAGCTCAAGCAAACAATGGACAAGTACAATACCGTCTGCAAGGAGGCTCTCTCTCCAGAACACAAGAGAGAAAAAGCGAATCGTAAGGCAGCCATTGAGGCAGCAGAAGCAGCTCAAAAGATTGCTGAATGGGAATCACAAAAAAGGAGGAATGCAGAACTGAACGCCCTTAAAGAATCTAAAGAGAGAAAGAAAGCATCAGAAGCAAGGGCCTGTGATATCAGTTATAGAAAATACACAATTGAGGAAATTGAATCAGCAACAAATAATTTTTCGATATCTTGCAAGATCGGAGAAGGAGGTTATGGTCCAGTTTACAAAGGGGAACTGGACCATACACCAGTGGCGATAAAAGCTCTGCGTCCAGATGCAGCTCAAGGACAGTCACAGTTCCAGCAAGAGTACATGGCTAATGGGAGCTTAGAAGAACGTATCTTCAAGAGAGGTAACACCCTAGTTATTCCTTGGCAACTAAGGTTCAGAATCGCTGCTGAAATCTGCACTGGGCTTTTGTTTCTTCATCAAACCAAGCCAGAGCCCCTAGTACACCGTGACCTCAAACCTGCTAACATTTTGCTAGACCATAACTATGTTAGCAAGATTAGTGATGTAGGTTTGGCCAGACTTGTCCCTCCATCAGTAGCTGATTCTATCACTCAGTATCGCATTACATCAGCAGCCGGAACATTCTGTTATATTGATCCAGAGTATCAGCAAACTGGTATGCTGGGAAGAAAATCTGATGTCTACTCACTTGGGGTTATGCTTTTACAACTAATCACAGCCAAGTCACCAATGGGTTTGACTTATCATGTTGAGAAAGCTATTAAGGAAGGGAATTTCACTGAGATGCTTGACCCTGCTGTCCCTGACTGGCCAGTTGAGGAAACCTTAGAGTTTGCCAAGCTAGCTTTACAGTGTGCTCAAATGAAACGAAAAGACAGACCGGATCTTGGGAAGGTTGTGCTACCTGAGTTTAACAGATTGAGAGCATTTGCCGAAGATAGCATGAATTCTGTCATGCCGGGTGGTGGTAAAGAACTCCTATCAAGACAAGACCCGATTTCCACAAGACAAGATGTGATCAGTGATCCCAATCTACCGCATTCTGGATATGATAGCTAG

Protein Analysis

711

Amino Acids

79.6

Weight (kDa)

8.23

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 14 - 159 8.7e-09 Universal stress protein family
Pkinase PF00069 468 - 655 3.1e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 470 - 658 2.9e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1874
Acc36I ACCTGC 1 cut(s) 1582
AccB1I GGYRCC 1 cut(s) 448
AccI GTMKAC 2 cut(s) 869, 1758
AccII CGCG 1 cut(s) 261
AciI CCGC 3 cut(s) 259, 646, 2114
AclWI GGATC 4 cut(s) 384, 1706, 1968, 2096
AcoI YGGCCR 3 cut(s) 348, 1629, 1886
AcsI RAATTY 4 cut(s) 697, 920, 1849, 2023
AfaI GTAC 5 cut(s) 372, 517, 1037, 1415, 1557
AfiI CCNNNNNNNGG 3 cut(s) 1964, 2042, 2120
AflIII ACRYGT 2 cut(s) 635, 780
AgsI TTSAA 9 cut(s) 357, 403, 442, 671, 834, 936, 941, 1265, 1450
AjiI CACGTC 1 cut(s) 867
AjnI CCWGG 1 cut(s) 51
AloI GAACNNNNNNTCC 2 cut(s) 845, 877
Alw21I GWGCWC 4 cut(s) 635, 908, 1017, 1936
AlwI GGATC 4 cut(s) 384, 1706, 1968, 2096
AlwNI CAGNNNCTG 2 cut(s) 737, 1655
AoxI GGCC 7 cut(s) 48, 121, 348, 394, 1221, 1629, 1886
ApeKI GCWGC 9 cut(s) 188, 533, 556, 1100, 1112, 1121, 1382, 1499, 1688
ApoI RAATTY 4 cut(s) 697, 920, 1849, 2023
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 5 cut(s) 49, 121, 1221, 1319, 1342
AsuC2I CCSGG 1 cut(s) 2037
AsuHPI GGTGA 4 cut(s) 372, 672, 720, 1794
AsuNHI GCTAGC 1 cut(s) 1918
AvaII GGWCC 2 cut(s) 1319, 1342
BaeGI GKGCMC 1 cut(s) 451
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BalI TGGCCA 3 cut(s) 350, 1631, 1888
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 3 cut(s) 908, 1017, 1551
Bbv12I GWGCWC 4 cut(s) 635, 908, 1017, 1936
BbvCI CCTCAGC 2 cut(s) 928, 1004
BbvI GCAGC 9 cut(s) 200, 520, 568, 1112, 1124, 1133, 1394, 1486, 1700
BccI CCATC 4 cut(s) 193, 391, 794, 1654
BciT130I CCWGG 1 cut(s) 53
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 3 cut(s) 103, 333, 2094
BcnI CCSGG 1 cut(s) 2037
BfaI CTAG 8 cut(s) 618, 651, 1010, 1466, 1553, 1589, 1919, 2134
BfmI CTRYAG 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 1582
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 1109
BisI GCNGC 9 cut(s) 189, 534, 557, 1101, 1113, 1122, 1383, 1500, 1689
BlpI GCTNAGC 1 cut(s) 907
BlsI GCNGC 9 cut(s) 190, 535, 558, 1102, 1114, 1123, 1384, 1501, 1690
Bme1390I CCNGG 2 cut(s) 53, 2037
Bme18I GGWCC 2 cut(s) 1319, 1342
BmgBI CACGTC 1 cut(s) 867
BmgT120I GGNCC 5 cut(s) 49, 121, 1221, 1319, 1342
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 2 cut(s) 53, 2037
BmrI ACTGGG 1 cut(s) 1524
BmsI GCATC 3 cut(s) 1217, 1369, 1851
BmtI GCTAGC 1 cut(s) 1922
BmuI ACTGGG 1 cut(s) 1524
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
BpmI CTGGAG 1 cut(s) 1050
Bpu10I CCTNAGC 2 cut(s) 928, 1004
Bpu1102I GCTNAGC 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 1001, 1371
BpuMI CCSGG 1 cut(s) 2037
BsaBI GATNNNNATC 1 cut(s) 2106
BsaJI CCNNGG 3 cut(s) 51, 117, 1475
BsaWI WCCGGW 2 cut(s) 451, 1957
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 3 cut(s) 1964, 2042, 2120
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 8 cut(s) 513, 1322, 1344, 1352, 1519, 1735, 1889, 1889
Bse3DI GCAATG 3 cut(s) 134, 298, 711
Bse8I GATNNNNATC 1 cut(s) 2106
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 3 cut(s) 51, 117, 1475
BseGI GGATG 1 cut(s) 979
BseJI GATNNNNATC 1 cut(s) 2106
BseLI CCNNNNNNNGG 3 cut(s) 1964, 2042, 2120
BseMI GCAATG 3 cut(s) 134, 298, 711
BseMII CTCAG 9 cut(s) 855, 894, 921, 942, 981, 995, 1682, 1848, 1974
BseNI ACTGG 8 cut(s) 513, 1322, 1344, 1352, 1519, 1735, 1889, 1889
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 9 cut(s) 200, 520, 568, 1112, 1124, 1133, 1394, 1486, 1700
BseYI CCCAGC 2 cut(s) 191, 1738
BsgI GTGCAG 1 cut(s) 1495
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 7 cut(s) 50, 123, 350, 396, 1223, 1631, 1888
BshNI GGYRCC 1 cut(s) 448
BsiHKAI GWGCWC 4 cut(s) 635, 908, 1017, 1936
BsiSI CCGG 6 cut(s) 393, 452, 610, 1692, 1958, 2036
BslFI GGGAC 3 cut(s) 305, 1625, 1862
BslI CCNNNNNNNGG 3 cut(s) 1964, 2042, 2120
BsmFI GGGAC 3 cut(s) 305, 1625, 1862
BsmI GAATGC 2 cut(s) 1168, 2116
BsnI GGCC 7 cut(s) 50, 123, 350, 396, 1223, 1631, 1888
Bsp1286I GDGCHC 6 cut(s) 451, 635, 908, 1017, 1551, 1936
Bsp143I GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
Bsp1720I GCTNAGC 1 cut(s) 907
BspACI CCGC 3 cut(s) 259, 646, 2114
BspANI GGCC 7 cut(s) 50, 123, 350, 396, 1223, 1631, 1888
BspCNI CTCAG 9 cut(s) 854, 893, 920, 941, 982, 996, 1681, 1849, 1975
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 450
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 1582
BspOI GCTAGC 1 cut(s) 1922
BspPI GGATC 4 cut(s) 384, 1706, 1968, 2096
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 3 cut(s) 134, 298, 711
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 8 cut(s) 513, 1322, 1344, 1352, 1519, 1735, 1889, 1889
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 3 cut(s) 51, 117, 1475
BssMI GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
BssT1I CCWWGG 2 cut(s) 117, 1475
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 7 cut(s) 46, 304, 1045, 1395, 1401, 1562, 1929
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 4 cut(s) 615, 656, 764, 1920
BstF5I GGATG 1 cut(s) 979
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 9 cut(s) 106, 210, 336, 392, 1302, 1714, 1963, 2097, 2104
BstMBI GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
BstMWI GCNNNNNNNGC 7 cut(s) 267, 1109, 1118, 1121, 1214, 1364, 1919
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 51, 2035
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 9 cut(s) 200, 520, 568, 1112, 1124, 1133, 1394, 1486, 1700
BstX2I RGATCY 2 cut(s) 389, 1960
BstYI RGATCY 2 cut(s) 389, 1960
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 7 cut(s) 50, 123, 350, 396, 1223, 1631, 1888
BtrI CACGTC 1 cut(s) 867
BtsCI GGATG 1 cut(s) 979
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 7 cut(s) 51, 469, 1359, 1512, 1854, 1934, 2104
BveI ACCTGC 1 cut(s) 1582
Cac8I GCNNGC 4 cut(s) 615, 656, 764, 1920
CaiI CAGNNNCTG 2 cut(s) 737, 1655
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 5 cut(s) 49, 121, 1221, 1319, 1342
CseI GACGC 1 cut(s) 1361
Csp6I GTAC 5 cut(s) 371, 516, 1036, 1414, 1556
CviQI GTAC 5 cut(s) 371, 516, 1036, 1414, 1556
DpnI GATC 9 cut(s) 105, 209, 335, 391, 1301, 1713, 1962, 2096, 2103
DpnII GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
DrdI GACNNNNNNGTC 1 cut(s) 1874
DseDI GACNNNNNNGTC 1 cut(s) 1874
EaeI YGGCCR 3 cut(s) 348, 1629, 1886
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 2 cut(s) 906, 1015
Eco130I CCWWGG 2 cut(s) 117, 1475
Eco24I GRGCYC 3 cut(s) 908, 1017, 1551
Eco32I GATATC 2 cut(s) 1231, 1290
Eco47I GGWCC 2 cut(s) 1319, 1342
Eco53kI GAGCTC 2 cut(s) 906, 1015
EcoICRI GAGCTC 2 cut(s) 906, 1015
EcoO109I RGGNCCY 1 cut(s) 1221
EcoRI GAATTC 1 cut(s) 2023
EcoRII CCWGG 1 cut(s) 51
EcoRV GATATC 2 cut(s) 1231, 1290
EcoT14I CCWWGG 2 cut(s) 117, 1475
EcoT38I GRGCYC 3 cut(s) 908, 1017, 1551
ErhI CCWWGG 2 cut(s) 117, 1475
FaqI GGGAC 3 cut(s) 305, 1625, 1862
FbaI TGATCA 3 cut(s) 103, 333, 2094
FblI GTMKAC 2 cut(s) 869, 1758
Fnu4HI GCNGC 9 cut(s) 189, 534, 557, 1101, 1113, 1122, 1383, 1500, 1689
FokI GGATG 1 cut(s) 986
FriOI GRGCYC 3 cut(s) 908, 1017, 1551
Fsp4HI GCNGC 9 cut(s) 189, 534, 557, 1101, 1113, 1122, 1383, 1500, 1689
FspBI CTAG 8 cut(s) 618, 651, 1010, 1466, 1553, 1589, 1919, 2134
GlaI GCGC 1 cut(s) 262
GluI GCNGC 9 cut(s) 189, 534, 557, 1101, 1113, 1122, 1383, 1500, 1689
GsaI CCCAGC 2 cut(s) 195, 1742
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 7 cut(s) 50, 123, 350, 396, 1223, 1631, 1888
HapII CCGG 6 cut(s) 393, 452, 610, 1692, 1958, 2036
HgaI GACGC 1 cut(s) 1361
HhaI GCGC 1 cut(s) 263
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HpaII CCGG 6 cut(s) 393, 452, 610, 1692, 1958, 2036
HphI GGTGA 4 cut(s) 372, 672, 720, 1794
Hpy166II GTNNAC 6 cut(s) 380, 518, 870, 1327, 1558, 1759
Hpy188III TCNNGA 9 cut(s) 387, 813, 882, 1067, 1376, 1450, 1715, 2061, 2121
Hpy8I GTNNAC 6 cut(s) 380, 518, 870, 1327, 1558, 1759
Hpy99I CGWCG 2 cut(s) 755, 871
HpyAV CCTTC 4 cut(s) 798, 1301, 1838, 1963
HpyCH4III ACNGT 7 cut(s) 46, 304, 1045, 1395, 1401, 1562, 1929
HpyCH4IV ACGT 5 cut(s) 342, 637, 750, 866, 1441
HpyF10VI GCNNNNNNNGC 7 cut(s) 267, 1109, 1118, 1121, 1214, 1364, 1919
HpySE526I ACGT 5 cut(s) 342, 637, 750, 866, 1441
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 3 cut(s) 103, 333, 2094
Kzo9I GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
LmnI GCTCC 5 cut(s) 144, 172, 541, 866, 1428
Lsp1109I GCAGC 9 cut(s) 200, 520, 568, 1112, 1124, 1133, 1394, 1486, 1700
LweI GCATC 3 cut(s) 1217, 1369, 1851
MaeI CTAG 8 cut(s) 618, 651, 1010, 1466, 1553, 1589, 1919, 2134
MaeII ACGT 5 cut(s) 342, 637, 750, 866, 1441
MalI GATC 9 cut(s) 105, 209, 335, 391, 1301, 1713, 1962, 2096, 2103
MboI GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
MboII GAAGA 8 cut(s) 242, 251, 989, 1438, 1448, 1520, 1755, 2024
MfeI CAATTG 3 cut(s) 357, 936, 1251
MflI RGATCY 2 cut(s) 389, 1960
MhlI GDGCHC 6 cut(s) 451, 635, 908, 1017, 1551, 1936
MlsI TGGCCA 3 cut(s) 350, 1631, 1888
MluNI TGGCCA 3 cut(s) 350, 1631, 1888
MlyI GAGTC 2 cut(s) 221, 818
Mox20I TGGCCA 3 cut(s) 350, 1631, 1888
MscI TGGCCA 3 cut(s) 350, 1631, 1888
MseI TTAA 3 cut(s) 1182, 1839, 1989
MslI CAYNNNNRTG 2 cut(s) 1599, 1859
Msp20I TGGCCA 3 cut(s) 350, 1631, 1888
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 6 cut(s) 393, 452, 610, 1692, 1958, 2036
MspR9I CCNGG 2 cut(s) 53, 2037
MunI CAATTG 3 cut(s) 357, 936, 1251
Mva1269I GAATGC 2 cut(s) 1168, 2116
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 7 cut(s) 267, 1109, 1118, 1121, 1214, 1364, 1919
NciI CCSGG 1 cut(s) 2037
NdeII GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
NheI GCTAGC 1 cut(s) 1918
NlaIV GGNNCC 1 cut(s) 450
NmuCI GTSAC 7 cut(s) 212, 298, 678, 956, 1395, 1562, 1800
NspI RCATGY 1 cut(s) 784
PciI ACATGT 1 cut(s) 780
PctI GAATGC 2 cut(s) 1168, 2116
PfeI GAWTC 9 cut(s) 408, 581, 623, 1090, 1145, 1187, 1265, 1494, 1658
PkrI GCNGC 9 cut(s) 190, 535, 558, 1102, 1114, 1123, 1384, 1501, 1690
PleI GAGTC 2 cut(s) 221, 817
PpsI GAGTC 2 cut(s) 221, 817
PscI ACATGT 1 cut(s) 780
Psp124BI GAGCTC 2 cut(s) 908, 1017
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 2 cut(s) 191, 1738
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 5 cut(s) 49, 121, 1221, 1319, 1342
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 2 cut(s) 737, 1655
PsuI RGATCY 2 cut(s) 389, 1960
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 5 cut(s) 372, 517, 1037, 1415, 1557
RsaNI GTAC 5 cut(s) 371, 516, 1036, 1414, 1556
RseI CAYNNNNRTG 2 cut(s) 1599, 1859
SacI GAGCTC 2 cut(s) 908, 1017
SalI GTCGAC 1 cut(s) 868
SaqAI TTAA 3 cut(s) 1182, 1839, 1989
SatI GCNGC 9 cut(s) 189, 534, 557, 1101, 1113, 1122, 1383, 1500, 1689
Sau3AI GATC 9 cut(s) 103, 207, 333, 389, 1299, 1711, 1960, 2094, 2101
Sau96I GGNCC 5 cut(s) 49, 121, 1221, 1319, 1342
SchI GAGTC 2 cut(s) 221, 818
ScrFI CCNGG 2 cut(s) 53, 2037
SduI GDGCHC 6 cut(s) 451, 635, 908, 1017, 1551, 1936
SfaNI GCATC 3 cut(s) 1217, 1369, 1851
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 1319, 1342
SmiMI CAYNNNNRTG 2 cut(s) 1599, 1859
SmlI CTYRAG 2 cut(s) 1016, 1386
SmoI CTYRAG 2 cut(s) 1016, 1386
SsiI CCGC 3 cut(s) 259, 646, 2114
SspI AATATT 1 cut(s) 830
SspMI CTAG 8 cut(s) 618, 651, 1010, 1466, 1553, 1589, 1919, 2134
SstI GAGCTC 2 cut(s) 908, 1017
StyD4I CCNGG 2 cut(s) 51, 2035
StyI CCWWGG 2 cut(s) 117, 1475
TaaI ACNGT 7 cut(s) 46, 304, 1045, 1395, 1401, 1562, 1929
TaiI ACGT 5 cut(s) 345, 640, 753, 869, 1444
TaqI TCGA 3 cut(s) 869, 946, 1286
TatI WGTACW 4 cut(s) 515, 1035, 1413, 1555
TfiI GAWTC 9 cut(s) 408, 581, 623, 1090, 1145, 1187, 1265, 1494, 1658
Tru1I TTAA 3 cut(s) 1182, 1839, 1989
Tru9I TTAA 3 cut(s) 1182, 1839, 1989
TscAI CASTG 7 cut(s) 51, 469, 1359, 1519, 1861, 1934, 2104
TseFI GTSAC 7 cut(s) 212, 298, 678, 956, 1395, 1562, 1800
TseI GCWGC 9 cut(s) 188, 533, 556, 1100, 1112, 1121, 1382, 1499, 1688
Tsp45I GTSAC 7 cut(s) 212, 298, 678, 956, 1395, 1562, 1800
TspDTI ATGAA 7 cut(s) 309, 722, 933, 990, 1520, 1955, 2036
TspRI CASTG 7 cut(s) 51, 469, 1359, 1519, 1861, 1934, 2104
VpaK11BI GGWCC 2 cut(s) 1319, 1342
XapI RAATTY 4 cut(s) 697, 920, 1849, 2023
XceI RCATGY 1 cut(s) 784
XcmI CCANNNNNNNNNTGG 1 cut(s) 1804
XmiI GTMKAC 2 cut(s) 869, 1758
XspI CTAG 8 cut(s) 618, 651, 1010, 1466, 1553, 1589, 1919, 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.